Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

23987870 .. 23991968

Gene Structure 4099 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g31211.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGAAAGCTAGAGAAGCTTCAAAAGGTGGCGAGTTAAGCATGTCGGAGAAGCAGTCTCCGAACGGAGGTGAAGTTATAGTGGAATTTCAAAGAGAAGAGACCAATGAAGCAAAGTGGTCTTCTTCAAAGGGCTCAGAATCTTCAGCTCCCAAGCAAAGCAAGGTGACCACTGGGTCTAGTGGTACTGGTTTCTCTAAGCCAGTTTCACCGGATATTTCAAGAGCCAATCCCTACTCAGGTAAACCTCCAAGAGCCCCCAACCGCAATGAGAGCATCAATAGGAGAAAGTCATTTAACAGGTCAGTTACTAAACCCAAGTCAAGATTTGGTGAACCATCGCCTGTTATGTATGAGGATGGTGGTTTAGATCAGGTTGGTGCAAGTTCCCCTTATAGGAGTTCATTTAGTAAGGGTTCCCCCTATAACCTATCGGGAGCTAAGGCTACATCCATCTCTTCGGCTAAGAATGCGCCGGCATCTCCGGGCAGGACTAATGAAAAGGAGGATGAAGAGATTTACAAGAAGGTTAAATTGAGTAGAGATAAGCGTGGGAAAGTGAACAAGAAACTCTTGGTTGAGTTGATTGTATTTCTATGCATTTTGAGTTGTTTACTGGCTAGCTTGATTGTCCATGAGCTAAGGCATTCTATGGTTTGGGGATTGGAGATTTGGAAATGGTGTGTGCTTGTCATGGTGATATTTAGTGGTATGTTGGTTACCAATTGGGTTATGCATTTCATAGTTTTTTTGATCGAGTGCAACTTTTTGCTTCGGAAGAAGGTGTTGTATTTTGTTCATGGAATGAAGAAGAGTGTTCAGGTTTTCTTGTGGCTGTCTCTGGTTCTTCTCACATGGCTGCTGTTGTTCAACCGTGGGGTTGAGAGGTCAGAGGCTTCCACCAAGATATTGAAATATGTTACATGGACTATTGTTTCAGTTCTCATTGGCGCATTTTTATGGTTGTTGAAAACATTGTTGCTCAAGATCTTGGCATCTAGTTTCCATGTTAACACCTTCTTTGATAGAATTCAAGAATCAATCTTCCACCAGTACGTGCTTCAGACACTGTCGGGGCATCCACTTATGGAGGAGGCTGATGGGAGTGGTGGGAGATCACCAAGCACTAGCAAACTGAGTTACAGAGCGACTAAGAAAGCAAAAGCAGGGAAGGAGAAAGAGGTCATTGACATGACAAAGCTTCAAAAGATGAAGCAAGAGAAGGTTTCATCTTGGACCATGAAGGTATTGGTGGATGCAGTTACTAGTTCAGGGCTGTCCACCATCTCTCATACATTGGATGGGGTGGACGGTCTAAATGCACAAAGAGATATTGAGATTACCAGTGAGATGGAAGCAACTGCTGCTGCCTATGACATATTCCTTCATGTCGCACCGCCTGGTGCCAAGTACATAGAGGAGTGGGATCTCTTAAGGTTCATGATTGAGGAAGAGGTTGATCTTGTGTGGCCGCTGATTGACGTAGCAAAGACTGGACAAGTTGACAGAAAAACTCTAACAGAGTGGGTGATAAAGGTGTTCAATGGTCGTAAAGCACTAGCACATTCCCTAACAGATACCAAAACAGCCGTGAAGCAATTGAACAAACTTGTCACTACCGTCTTGGTTATTATCACCACTGTAGTGTGGCTTCTGTTGGTGGAAATTGCAAATACAAAAGTACTCGTCTTCCTCTCATCACAGCTCGTACTGGCAGCTTTTATGTTTGGGAACACTTGCAAGACTATATTTGAAGCTATTATCTTTGTATTTGTAATGCATCCATTTGATGTTGGCGATCGCTGTGTTGTTGAGGGTGAGGAGTTGATGGTTGAGGAGATGAACATCTTAACTACAGTCTTCTTGAAACTCAATCAAGAGAAGGTATACTATCCGAATTCAGTTCTATCTACGAAACCCATCAGCAATTATTACAGAAGTTCAAACATGGGTGATATTGTGGAGTTCTCAATTGCTTTTACTACACCAGTGAAGAAGATAGGCCAGCTGAAAGAGAAAGTACAAGAGTATGTGGAGGGGAACCCAACACTTTGGCATCCAAACCATGCGGTGGTGGTGGTTGAGATCGAAAATATGAATAAGCTAAAGATGGCCCTCTATTTTTGTCACACTATGAACTTTCAAGAATGGGTTGAAAAGCAAAAGCGCAGAAGTGAAATGGTCATGGCGTTGAAGAGATGTTTCGATGAGCTACAGATTACGTACTATCTACTGCCCCTTGAAGTTCGTCTCACTGAAACAAATGTCACCAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

759

Amino Acids

85.61

Weight (kDa)

9.21

Isoelectric Point (pI)

41.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	587 - 644	7.3e-08	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	172
AccB1I	GGYRCC	1 cut(s)	1400
AccB7I	CCANNNNNTGG	1 cut(s)	2068
AccI	GTMKAC	1 cut(s)	1884
AciI	CCGC	4 cut(s)	262, 1394, 1469, 2066
AclWI	GGATC	1 cut(s)	1431
AcoI	YGGCCR	1 cut(s)	1466
AcsI	RAATTY	3 cut(s)	83, 1026, 1894
AcuI	CTGAAG	2 cut(s)	126, 1043
AfaI	GTAC	7 cut(s)	184, 1052, 1409, 1680, 1707, 2019, 2222
AfiI	CCNNNNNNNGG	4 cut(s)	65, 236, 393, 2068
AflII	CTTAAG	1 cut(s)	1429
AhlI	ACTAGT	1 cut(s)	1262
AjnI	CCWGG	1 cut(s)	1396
AleI	CACNNNNGTG	1 cut(s)	1640
Alw26I	GTCTC	4 cut(s)	60, 92, 840, 2252
AlwI	GGATC	1 cut(s)	1431
AlwNI	CAGNNNCTG	1 cut(s)	1066
AoxI	GGCC	3 cut(s)	1466, 1999, 2109
ApeKI	GCWGC	4 cut(s)	856, 1361, 1364, 1712
ApoI	RAATTY	3 cut(s)	83, 1026, 1894
AsiSI	GCGATCGC	1 cut(s)	1798
AspLEI	GCGC	3 cut(s)	472, 950, 2166
AspS9I	GGNCC	2 cut(s)	1233, 2110
AsuC2I	CCSGG	1 cut(s)	483
AsuNHI	GCTAGC	1 cut(s)	617
AvaII	GGWCC	1 cut(s)	1233
BanI	GGYRCC	1 cut(s)	1400
BanII	GRGCYC	2 cut(s)	134, 256
BarI	GAAGNNNNNNTAC	2 cut(s)	1632, 1664
BbsI	GAAGAC	3 cut(s)	111, 1678, 1849
BbvI	GCAGC	4 cut(s)	843, 1348, 1351, 1724
BceAI	ACGGC	1 cut(s)	1571
BciT130I	CCWGG	1 cut(s)	1398
BcnI	CCSGG	1 cut(s)	483
BcoDI	GTCTC	4 cut(s)	60, 92, 840, 2252
BcuI	ACTAGT	1 cut(s)	1262
BfaI	CTAG	7 cut(s)	9, 177, 618, 996, 1125, 1263, 1556
BfmI	CTRYAG	3 cut(s)	1638, 1851, 2210
BfrI	CTTAAG	1 cut(s)	1429
BglII	AGATCT	1 cut(s)	984
BisI	GCNGC	5 cut(s)	857, 1362, 1365, 1469, 1713
BlsI	GCNGC	5 cut(s)	858, 1363, 1366, 1470, 1714
BmcAI	AGTACT	1 cut(s)	1680
Bme1390I	CCNGG	2 cut(s)	483, 1398
Bme18I	GGWCC	1 cut(s)	1233
BmgT120I	GGNCC	2 cut(s)	1233, 2110
BmiI	GGNNCC	3 cut(s)	415, 1402, 2039
BmrFI	CCNGG	2 cut(s)	483, 1398
BmrI	ACTGGG	1 cut(s)	180
BmsI	GCATC	7 cut(s)	282, 485, 1001, 1084, 1243, 1786, 2062
BmtI	GCTAGC	1 cut(s)	621
BmuI	ACTGGG	1 cut(s)	180
BpiI	GAAGAC	3 cut(s)	111, 1678, 1849
Bpu10I	CCTNAGC	2 cut(s)	438, 638
BpuEI	CTTGAG	1 cut(s)	965
BpuMI	CCSGG	1 cut(s)	483
BsaAI	YACGTR	2 cut(s)	1054, 2220
BsaBI	GATNNNNATC	1 cut(s)	1841
BsaI	GGTCTC	1 cut(s)	92
BsaJI	CCNNGG	1 cut(s)	871
BsaWI	WCCGGW	1 cut(s)	208
BsaXI	ACNNNNNCTCC	4 cut(s)	38, 57, 68, 87
Bsc4I	CCNNNNNNNGG	4 cut(s)	65, 236, 393, 2068
Bse118I	RCCGGY	1 cut(s)	472
Bse1I	ACTGG	9 cut(s)	175, 190, 200, 618, 1048, 1341, 1495, 1713, 1985
Bse3DI	GCAATG	1 cut(s)	271
Bse8I	GATNNNNATC	1 cut(s)	1841
BseBI	CCWGG	1 cut(s)	1398
BseDI	CCNNGG	1 cut(s)	871
BseGI	GGATG	8 cut(s)	361, 446, 511, 1075, 1258, 1303, 1777, 2053
BseJI	GATNNNNATC	1 cut(s)	1841
BseLI	CCNNNNNNNGG	4 cut(s)	65, 236, 393, 2068
BseMI	GCAATG	1 cut(s)	271
BseMII	CTCAG	3 cut(s)	147, 249, 1124
BseNI	ACTGG	9 cut(s)	175, 190, 200, 618, 1048, 1341, 1495, 1713, 1985
BseRI	GAGGAG	4 cut(s)	1103, 1430, 1832, 1847
BseXI	GCAGC	4 cut(s)	843, 1348, 1351, 1724
Bsh1285I	CGRYCG	1 cut(s)	1798
BshFI	GGCC	3 cut(s)	1468, 2001, 2111
BshNI	GGYRCC	1 cut(s)	1400
BsiEI	CGRYCG	1 cut(s)	1798
BsiSI	CCGG	3 cut(s)	209, 473, 482
BslI	CCNNNNNNNGG	4 cut(s)	65, 236, 393, 2068
BsmAI	GTCTC	4 cut(s)	60, 92, 840, 2252
BsmBI	CGTCTC	1 cut(s)	2252
BsmI	GAATGC	2 cut(s)	472, 643
BsnI	GGCC	3 cut(s)	1468, 2001, 2111
Bso31I	GGTCTC	1 cut(s)	92
Bsp1286I	GDGCHC	2 cut(s)	134, 256
Bsp143I	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
BspACI	CCGC	4 cut(s)	262, 1394, 1469, 2066
BspANI	GGCC	3 cut(s)	1468, 2001, 2111
BspCNI	CTCAG	3 cut(s)	146, 248, 1125
BspHI	TCATGA	1 cut(s)	1437
BspLI	GGNNCC	3 cut(s)	415, 1402, 2039
BspOI	GCTAGC	1 cut(s)	621
BspPI	GGATC	1 cut(s)	1431
BspT107I	GGYRCC	1 cut(s)	1400
BspTI	CTTAAG	1 cut(s)	1429
BspTNI	GGTCTC	1 cut(s)	92
BsrDI	GCAATG	1 cut(s)	271
BsrFI	RCCGGY	1 cut(s)	472
BsrI	ACTGG	9 cut(s)	175, 190, 200, 618, 1048, 1341, 1495, 1713, 1985
BssAI	RCCGGY	1 cut(s)	472
BssECI	CCNNGG	1 cut(s)	871
BssMI	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
BssNAI	GTATAC	1 cut(s)	1885
Bst1107I	GTATAC	1 cut(s)	1885
Bst2UI	CCWGG	1 cut(s)	1398
Bst4CI	ACNGT	6 cut(s)	872, 1068, 1310, 1618, 1639, 1855
Bst6I	CTCTTC	6 cut(s)	90, 460, 504, 803, 1443, 2186
BstAFI	CTTAAG	1 cut(s)	1429
BstAPI	GCANNNNNTGC	1 cut(s)	1361
BstBAI	YACGTR	2 cut(s)	1054, 2220
BstC8I	GCNNGC	3 cut(s)	474, 619, 2003
BstDEI	CTNAG	8 cut(s)	133, 195, 235, 438, 462, 638, 1133, 1149
BstDSI	CCRYGG	1 cut(s)	871
BstEII	GGTNACC	2 cut(s)	163, 715
BstF5I	GGATG	8 cut(s)	361, 446, 511, 1075, 1258, 1303, 1777, 2053
BstHHI	GCGC	3 cut(s)	472, 950, 2166
BstKTI	GATC	8 cut(s)	370, 753, 987, 1115, 1426, 1459, 1798, 2085
BstMAI	GTCTC	4 cut(s)	60, 92, 840, 2252
BstMBI	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
BstMCI	CGRYCG	1 cut(s)	1798
BstMWI	GCNNNNNNNGC	5 cut(s)	14, 36, 270, 467, 1361
BstNI	CCWGG	1 cut(s)	1398
BstNSI	RCATGY	1 cut(s)	43
BstPI	GGTNACC	2 cut(s)	163, 715
BstSCI	CCNGG	2 cut(s)	481, 1396
BstSFI	CTRYAG	3 cut(s)	1638, 1851, 2210
BstSNI	TACGTA	1 cut(s)	2220
BstV1I	GCAGC	4 cut(s)	843, 1348, 1351, 1724
BstV2I	GAAGAC	3 cut(s)	111, 1678, 1849
BstX2I	RGATCY	2 cut(s)	984, 1423
BstXI	CCANNNNNNTGG	2 cut(s)	1348, 2049
BstYI	RGATCY	2 cut(s)	984, 1423
BstZ17I	GTATAC	1 cut(s)	1885
BsuRI	GGCC	3 cut(s)	1468, 2001, 2111
BtgI	CCRYGG	1 cut(s)	871
BtgZI	GCGATG	1 cut(s)	321
BtsCI	GGATG	8 cut(s)	361, 446, 511, 1075, 1258, 1303, 1777, 2053
BtsIMutI	CAGTG	6 cut(s)	168, 1064, 1348, 1635, 1992, 2250
Cac8I	GCNNGC	3 cut(s)	474, 619, 2003
CaiI	CAGNNNCTG	1 cut(s)	1066
CciI	TCATGA	1 cut(s)	1437
CfoI	GCGC	3 cut(s)	472, 950, 2166
Cfr10I	RCCGGY	1 cut(s)	472
Cfr13I	GGNCC	2 cut(s)	1233, 2110
Csp6I	GTAC	7 cut(s)	183, 1051, 1408, 1679, 1706, 2018, 2221
CviQI	GTAC	7 cut(s)	183, 1051, 1408, 1679, 1706, 2018, 2221
DdeI	CTNAG	8 cut(s)	133, 195, 235, 438, 462, 638, 1133, 1149
DpnI	GATC	8 cut(s)	369, 752, 986, 1114, 1425, 1458, 1797, 2084
DpnII	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
DrdI	GACNNNNNNGTC	1 cut(s)	172
DseDI	GACNNNNNNGTC	1 cut(s)	172
EaeI	YGGCCR	1 cut(s)	1466
Eam1104I	CTCTTC	6 cut(s)	90, 460, 504, 803, 1443, 2186
EarI	CTCTTC	6 cut(s)	90, 460, 504, 803, 1443, 2186
Eco105I	TACGTA	1 cut(s)	2220
Eco24I	GRGCYC	2 cut(s)	134, 256
Eco31I	GGTCTC	1 cut(s)	92
Eco47I	GGWCC	1 cut(s)	1233
Eco57I	CTGAAG	2 cut(s)	126, 1043
Eco91I	GGTNACC	2 cut(s)	163, 715
EcoO65I	GGTNACC	2 cut(s)	163, 715
EcoRI	GAATTC	2 cut(s)	1026, 1894
EcoRII	CCWGG	1 cut(s)	1396
EcoT22I	ATGCAT	3 cut(s)	599, 735, 1779
EcoT38I	GRGCYC	2 cut(s)	134, 256
Esp3I	CGTCTC	1 cut(s)	2252
FalI	AAGNNNNNCTT	4 cut(s)	373, 405, 554, 586
FblI	GTMKAC	1 cut(s)	1884
Fnu4HI	GCNGC	5 cut(s)	857, 1362, 1365, 1469, 1713
FokI	GGATG	8 cut(s)	368, 433, 518, 1062, 1265, 1310, 1764, 2040
FriOI	GRGCYC	2 cut(s)	134, 256
Fsp4HI	GCNGC	5 cut(s)	857, 1362, 1365, 1469, 1713
FspBI	CTAG	7 cut(s)	9, 177, 618, 996, 1125, 1263, 1556
GlaI	GCGC	3 cut(s)	471, 949, 2165
GluI	GCNGC	5 cut(s)	857, 1362, 1365, 1469, 1713
HaeIII	GGCC	3 cut(s)	1468, 2001, 2111
HapII	CCGG	3 cut(s)	209, 473, 482
HhaI	GCGC	3 cut(s)	472, 950, 2166
Hin6I	GCGC	3 cut(s)	470, 948, 2164
HinP1I	GCGC	3 cut(s)	470, 948, 2164
HincII	GTYRAC	2 cut(s)	1009, 1501
HindII	GTYRAC	2 cut(s)	1009, 1501
HindIII	AAGCTT	2 cut(s)	15, 1196
HinfI	GANTC	2 cut(s)	137, 1034
HpaI	GTTAAC	1 cut(s)	1009
HpaII	CCGG	3 cut(s)	209, 473, 482
Hpy166II	GTNNAC	9 cut(s)	242, 332, 559, 611, 1009, 1278, 1306, 1501, 1885
Hpy188I	TCNGA	7 cut(s)	46, 60, 136, 774, 889, 1062, 1893
Hpy188III	TCNNGA	9 cut(s)	219, 321, 432, 982, 1031, 1438, 1861, 1874, 2141
Hpy8I	GTNNAC	9 cut(s)	242, 332, 559, 611, 1009, 1278, 1306, 1501, 1885
HpyAV	CCTTC	8 cut(s)	517, 772, 1024, 1162, 1213, 1234, 1391, 1873
HpyCH4III	ACNGT	6 cut(s)	872, 1068, 1310, 1618, 1639, 1855
HpyCH4IV	ACGT	3 cut(s)	1053, 1479, 2219
HpyCH4V	TGCA	9 cut(s)	380, 597, 733, 759, 1256, 1319, 1667, 1737, 1777
HpyF10VI	GCNNNNNNNGC	5 cut(s)	14, 36, 270, 467, 1361
HpyF3I	CTNAG	8 cut(s)	133, 195, 235, 438, 462, 638, 1133, 1149
HpySE526I	ACGT	3 cut(s)	1053, 1479, 2219
HspAI	GCGC	3 cut(s)	470, 948, 2164
KroI	GCCGGC	1 cut(s)	472
KroNI	GCCGGC	1 cut(s)	474
KspAI	GTTAAC	1 cut(s)	1009
Kzo9I	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
LmnI	GCTCC	2 cut(s)	151, 434
Lsp1109I	GCAGC	4 cut(s)	843, 1348, 1351, 1724
LweI	GCATC	7 cut(s)	282, 485, 1001, 1084, 1243, 1786, 2062
MaeI	CTAG	7 cut(s)	9, 177, 618, 996, 1125, 1263, 1556
MaeII	ACGT	3 cut(s)	1053, 1479, 2219
MaeIII	GTNAC	9 cut(s)	163, 304, 715, 916, 1136, 1258, 1609, 2123, 2263
MalI	GATC	8 cut(s)	369, 752, 986, 1114, 1425, 1458, 1797, 2084
MboI	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
MfeI	CAATTG	3 cut(s)	721, 1595, 1968
MflI	RGATCY	2 cut(s)	984, 1423
MhlI	GDGCHC	2 cut(s)	134, 256
MluCI	AATT	9 cut(s)	83, 530, 721, 1026, 1595, 1662, 1894, 1924, 1968
MmeI	TCCRAC	1 cut(s)	24
Mph1103I	ATGCAT	3 cut(s)	599, 735, 1779
MroNI	GCCGGC	1 cut(s)	472
MseI	TTAA	6 cut(s)	35, 294, 528, 1008, 1430, 1847
MslI	CAYNNNNRTG	3 cut(s)	955, 1187, 1640
MspA1I	CMGCKG	2 cut(s)	1471, 2005
MspCI	CTTAAG	1 cut(s)	1429
MspI	CCGG	3 cut(s)	209, 473, 482
MspR9I	CCNGG	2 cut(s)	483, 1398
MunI	CAATTG	3 cut(s)	721, 1595, 1968
Mva1269I	GAATGC	2 cut(s)	472, 643
MvaI	CCWGG	1 cut(s)	1398
MwoI	GCNNNNNNNGC	5 cut(s)	14, 36, 270, 467, 1361
NaeI	GCCGGC	1 cut(s)	474
NciI	CCSGG	1 cut(s)	483
NdeII	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
NgoMIV	GCCGGC	1 cut(s)	472
NheI	GCTAGC	1 cut(s)	617
NlaIV	GGNNCC	3 cut(s)	415, 1402, 2039
NmuCI	GTSAC	4 cut(s)	163, 1609, 2123, 2263
NsiI	ATGCAT	3 cut(s)	599, 735, 1779
NspI	RCATGY	1 cut(s)	43
OliI	CACNNNNGTG	1 cut(s)	1640
PagI	TCATGA	1 cut(s)	1437
PctI	GAATGC	2 cut(s)	472, 643
PdiI	GCCGGC	1 cut(s)	474
PfeI	GAWTC	2 cut(s)	137, 1034
PflFI	GACNNNGTC	1 cut(s)	1066
PflMI	CCANNNNNTGG	1 cut(s)	2068
PkrI	GCNGC	5 cut(s)	858, 1363, 1366, 1470, 1714
Ple19I	CGATCG	1 cut(s)	1798
Ppu21I	YACGTR	2 cut(s)	1054, 2220
Psp6I	CCWGG	1 cut(s)	1396
PspEI	GGTNACC	2 cut(s)	163, 715
PspGI	CCWGG	1 cut(s)	1396
PspN4I	GGNNCC	3 cut(s)	415, 1402, 2039
PspPI	GGNCC	2 cut(s)	1233, 2110
PstNI	CAGNNNCTG	1 cut(s)	1066
PsuI	RGATCY	2 cut(s)	984, 1423
PsyI	GACNNNGTC	1 cut(s)	1066
PvuI	CGATCG	1 cut(s)	1798
PvuII	CAGCTG	1 cut(s)	2005
RgaI	GCGATCGC	1 cut(s)	1798
RsaI	GTAC	7 cut(s)	184, 1052, 1409, 1680, 1707, 2019, 2222
RsaNI	GTAC	7 cut(s)	183, 1051, 1408, 1679, 1706, 2018, 2221
RseI	CAYNNNNRTG	3 cut(s)	955, 1187, 1640
SaqAI	TTAA	6 cut(s)	35, 294, 528, 1008, 1430, 1847
SatI	GCNGC	5 cut(s)	857, 1362, 1365, 1469, 1713
Sau3AI	GATC	8 cut(s)	367, 750, 984, 1112, 1423, 1456, 1795, 2082
Sau96I	GGNCC	2 cut(s)	1233, 2110
ScaI	AGTACT	1 cut(s)	1680
ScrFI	CCNGG	2 cut(s)	483, 1398
SduI	GDGCHC	2 cut(s)	134, 256
SfaAI	GCGATCGC	1 cut(s)	1798
SfaNI	GCATC	7 cut(s)	282, 485, 1001, 1084, 1243, 1786, 2062
SfcI	CTRYAG	3 cut(s)	1638, 1851, 2210
SgfI	GCGATCGC	1 cut(s)	1798
SinI	GGWCC	1 cut(s)	1233
SmiMI	CAYNNNNRTG	3 cut(s)	955, 1187, 1640
SmlI	CTYRAG	2 cut(s)	980, 1429
SmoI	CTYRAG	2 cut(s)	980, 1429
SnaBI	TACGTA	1 cut(s)	2220
SpeI	ACTAGT	1 cut(s)	1262
Sse9I	AATT	9 cut(s)	83, 530, 721, 1026, 1595, 1662, 1894, 1924, 1968
SsiI	CCGC	4 cut(s)	262, 1394, 1469, 2066
SspMI	CTAG	7 cut(s)	9, 177, 618, 996, 1125, 1263, 1556
StyD4I	CCNGG	2 cut(s)	481, 1396
TaaI	ACNGT	6 cut(s)	872, 1068, 1310, 1618, 1639, 1855
TaiI	ACGT	3 cut(s)	1056, 1482, 2222
TaqI	TCGA	3 cut(s)	753, 2085, 2202
TasI	AATT	9 cut(s)	83, 530, 721, 1026, 1595, 1662, 1894, 1924, 1968
TatI	WGTACW	3 cut(s)	1407, 1678, 2017
TauI	GCSGC	1 cut(s)	1471
TfiI	GAWTC	2 cut(s)	137, 1034
Tru1I	TTAA	6 cut(s)	35, 294, 528, 1008, 1430, 1847
Tru9I	TTAA	6 cut(s)	35, 294, 528, 1008, 1430, 1847
TscAI	CASTG	6 cut(s)	175, 1071, 1348, 1642, 1992, 2257
TseFI	GTSAC	4 cut(s)	163, 1609, 2123, 2263
TseI	GCWGC	4 cut(s)	856, 1361, 1364, 1712
Tsp45I	GTSAC	4 cut(s)	163, 1609, 2123, 2263
TspGWI	ACGGA	1 cut(s)	78
TspRI	CASTG	6 cut(s)	175, 1071, 1348, 1642, 1992, 2257
Tth111I	GACNNNGTC	1 cut(s)	1066
Van91I	CCANNNNNTGG	1 cut(s)	2068
Vha464I	CTTAAG	1 cut(s)	1429
VpaK11BI	GGWCC	1 cut(s)	1233
XapI	RAATTY	3 cut(s)	83, 1026, 1894
XceI	RCATGY	1 cut(s)	43
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1243
XmiI	GTMKAC	1 cut(s)	1884
XspI	CTAG	7 cut(s)	9, 177, 618, 996, 1125, 1263, 1556
ZrmI	AGTACT	1 cut(s)	1680
Zsp2I	ATGCAT	3 cut(s)	599, 735, 1779

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.