Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Forward (+)

6585355 .. 6588966

Gene Structure 3612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G19520.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2229 bp

ATGGCTGAGAGGAGAGTCAGTAACGGAGAAGAAGTTGTTATTAATGTTTCAGATAAAGAAGATTCAAAAGATCCAAGAGCATCTCCTTCTTTCAATCCATTGGCTTCACCAGACTCCGATGCTGGGATTGAGAAATCGAAACCAGTGCCGCCCATAAGCATTCCTACTCCTGAAATCTATAAGTTCTCTGGTAGCGTTCATAAGCCACCTAAAATTCCAAGTCCTGAAGGTCTAGTTCGGAGGAAGTCTCTTTCGAGGTCAATTTACTCCAAGCCTAAGTCAAGGTTTGGGGAGCAACAGTCTTTTCGATACGATAGCACTAGAGAGGAAAATGGTGGAAGGTCCCTTAGGGAACAGTTTGGTGCTGGTTCATTTGCAAGGGGTTCTTTTGACAGGGCTTCTCCTAATAACAAATCCAATAGGAGCGTTGCGTCAGCAGCACTGAGTAAAGTTGCTGAGGAGGAACCGGATGAAAATGAAGAAATCTACAAAAAGGTTAAGCTACACCGAGTGAAGCGTAGCGGAATGAAACCTCTGGCCTTTCTTGAGTTGGTAGTGTTTATGGCCATTTTGGGGGCTTTAATTGTGAGTTTAACCATTGATGTGGTGAACAAGCATACCATTTGGGGGTTGGAATTTTGGAAATGGTGTGTGCTTGTGATGGTTACACTCAGCGGCATGTTGGTGACAAATTGGTTCATGCATTTTGTGGTGTTCATCATAGAAAAGAACTATCTTCTTCGGAAAAAAGTTTTGTACTTTGTGCATGGGTTGAAGAAGAATGTTCAAGTCTTCATTTGGTTTAGTCTGGTTCTGATTGCTTGGATATGTCTGTTCGATGGTGATGTTAAACGTACTCGTAAGACCAAGAGATTTCTAGACTTCATCACTTGGACTATTGTCTCTCTTCTCGTAGGGTCAATCCTTTTCTTGGTGAAGACGTTTGCCTTGAAAGTTCTTGCTTCAAAGTTCAACGTCAGAAACTTCTTTGAGAGGATTCAAGAGTCTGTTTTCCACCAATACGTTCTTCAGACTCTCTCGGGACCTCCGCTTATCGAAGAGGCAGAGAACGTTGGGCGCGTGCCAAGCACAGGTCACCTAAGTTTCACAAGAACCAAAGATGGAAAGGTGAAAGACAAGAAAGTGATCGATATGGGAAAGGTTCACAGGATGAAACAAGAGAAGGTCTCTGCTTGGACAATGCGTGTTTTGATCGAAGCCGTGGGAACTTCAGGTATATCAACCATATCCAGCACTCTAGACGAGGTCAACAATAAGAAAGAGAGAACAGATAAAGAGATAACAAATGAGATGGAGGCTGTTGCTGCTGCTTATGACGTCTTCAACAATGTCGCTAAGCCAAACCACAATTACATAGAAGAAGATGACTTATTGAGGTTCATGATAAAGGAAGAAGTAGACCTTGTACTCCCATTAATAGAAGATGCCGACACCGGAAAAATCACACGCAAGACTTTTACAGAATGGGTGGTTAATGTATACACAAGTCGGAAAACTATAGGGCATTCACTGAACGACACAAAAACAGCGGTTAAGCAGTTGGACAAACTTATAACTGGAATCTTGACCGTTATCACATTCATTGTTTGGATGGTACTTCTGGATATAGCATCGACCAAGCTTTTATTGGTCTTCTCCTCACAATTTTTGGGTCTTGCTTTCATGATCGGAAGTACTTGTAAGAATATCTTCGAATCCTTTATGTTCGTCTTTGTGATGCATCCTTATGACGTCGGAGATCGTTGCGTTGTCGACGGTGTTATGTTGTTGGTTGAAGAAATAGATCTTTTAACAACCGTGTTCCTCAAGATTGACAACGAGAAAGTGTTCTACCCAAATTCGGTTTTAATATCGAAACCGATAAGCAACTTCTACAGAAGTCCAGATATGGGAGATTATGTCGACTTCGGCATCGCATTCTCAACACCGGCCGAGAAAATCGGCTGTCTCAAGGGAAAAATCGGAGAATACTTGGTGGCAAACTCACAACATTGGTATCCAGAAGCACAGGTGATGGTGAGGGCAATTGAGAATATGAACAAGCTGGTATTGAATATACTGGTCCAACACACGATAAATTTCCAAGTATATGTAGAGAAGAGTTTAAGAAGAACCGCGCTGATCATCGCAATCAAGCGAATTCTCGAGGATCTTGAGATCGATTACACTCTCCTTCCTCAAGATGTCAATCTCACTGGTCACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0080167 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

742

Amino Acids

84.25

Weight (kDa)

9.02

Isoelectric Point (pI)

38.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	185 - 324	1.4e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	567 - 631	4.5e-08	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1574
AatII	GACGTC	2 cut(s)	1341, 1755
AccI	GTMKAC	4 cut(s)	1419, 1500, 1773, 1923
AccII	CGCG	2 cut(s)	1080, 2138
AciI	CCGC	6 cut(s)	149, 522, 675, 1049, 1550, 2136
AclI	AACGTT	1 cut(s)	1071
AclWI	GGATC	2 cut(s)	65, 2178
AcoI	YGGCCR	2 cut(s)	564, 1950
AcsI	RAATTY	5 cut(s)	213, 635, 1858, 2098, 2160
AcuI	CTGAAG	3 cut(s)	246, 1013, 1215
AcyI	GRCGYC	2 cut(s)	1338, 1752
AdeI	CACNNNGTG	1 cut(s)	511
AfaI	GTAC	5 cut(s)	758, 856, 1428, 1617, 1696
AfiI	CCNNNNNNNGG	7 cut(s)	123, 573, 627, 931, 1091, 1861, 1910
AjuI	GAANNNNNNNTTGG	2 cut(s)	1011, 1043
AluBI	AGCT	3 cut(s)	502, 1642, 2065
AluI	AGCT	3 cut(s)	502, 1642, 2065
Alw26I	GTCTC	4 cut(s)	252, 907, 1192, 1973
AlwI	GGATC	2 cut(s)	65, 2178
Ama87I	CYCGRG	2 cut(s)	1039, 2165
AoxI	GGCC	3 cut(s)	537, 564, 1950
ApeKI	GCWGC	3 cut(s)	437, 1325, 1328
ApoI	RAATTY	5 cut(s)	213, 635, 1858, 2098, 2160
AseI	ATTAAT	2 cut(s)	42, 1436
Asp700I	GAANNNNTTC	2 cut(s)	1709, 1847
AspLEI	GCGC	2 cut(s)	1080, 2140
AspS9I	GGNCC	3 cut(s)	342, 1043, 2083
AsuHPI	GGTGA	9 cut(s)	99, 619, 697, 854, 946, 1088, 1141, 2044, 2050
AsuII	TTCGAA	1 cut(s)	1713
AvaI	CYCGRG	2 cut(s)	1039, 2165
AvaII	GGWCC	3 cut(s)	342, 1043, 2083
AxyI	CCTNAGG	1 cut(s)	347
BaeI	ACNNNNGTAYC	2 cut(s)	2000, 2033
BalI	TGGCCA	1 cut(s)	566
BbsI	GAAGAC	4 cut(s)	784, 944, 1333, 1645
BbvCI	CCTCAGC	1 cut(s)	456
BbvI	GCAGC	3 cut(s)	449, 1312, 1315
BccI	CCATC	6 cut(s)	655, 833, 1115, 1306, 1606, 2029
BceAI	ACGGC	1 cut(s)	1205
BcgI	CGANNNNNNTGC	2 cut(s)	127, 161
BciVI	GTATCC	1 cut(s)	2028
BclI	TGATCA	1 cut(s)	2142
BcoDI	GTCTC	4 cut(s)	252, 907, 1192, 1973
BfaI	CTAG	4 cut(s)	233, 321, 878, 1259
BfmI	CTRYAG	2 cut(s)	1518, 1894
BfuI	GTATCC	1 cut(s)	2028
BglII	AGATCT	1 cut(s)	1804
BisI	GCNGC	5 cut(s)	149, 438, 676, 1326, 1329
BlpI	GCTNAGC	1 cut(s)	1356
BlsI	GCNGC	5 cut(s)	150, 439, 677, 1327, 1330
BmcAI	AGTACT	1 cut(s)	1696
Bme18I	GGWCC	3 cut(s)	342, 1043, 2083
BmeT110I	CYCGRG	2 cut(s)	1039, 2165
BmgT120I	GGNCC	3 cut(s)	342, 1043, 2083
BmiI	GGNNCC	3 cut(s)	344, 465, 1044
BmsI	GCATC	7 cut(s)	89, 109, 1435, 1640, 1728, 1750, 1941
BoxI	GACNNNNGTC	1 cut(s)	899
BpiI	GAAGAC	4 cut(s)	784, 944, 1333, 1645
BplI	GAGNNNNNCTC	4 cut(s)	232, 264, 1172, 1204
Bpu10I	CCTNAGC	1 cut(s)	456
Bpu1102I	GCTNAGC	1 cut(s)	1356
Bpu14I	TTCGAA	1 cut(s)	1713
BpuEI	CTTGAG	5 cut(s)	566, 1811, 1955, 2184, 2195
Bsa29I	ATCGAT	2 cut(s)	1149, 2181
BsaBI	GATNNNNATC	1 cut(s)	2208
BsaHI	GRCGYC	2 cut(s)	1338, 1752
BsaI	GGTCTC	1 cut(s)	1192
BsaJI	CCNNGG	1 cut(s)	1221
BsaWI	WCCGGW	2 cut(s)	466, 1454
BsaXI	ACNNNNNCTCC	7 cut(s)	18, 34, 48, 284, 314, 1905, 1935
Bsc4I	CCNNNNNNNGG	7 cut(s)	123, 573, 627, 931, 1091, 1861, 1910
Bse118I	RCCGGY	1 cut(s)	1948
Bse1I	ACTGG	4 cut(s)	143, 1582, 2085, 2221
Bse21I	CCTNAGG	1 cut(s)	347
Bse8I	GATNNNNATC	1 cut(s)	2208
BseCI	ATCGAT	2 cut(s)	1149, 2181
BseDI	CCNNGG	1 cut(s)	1221
BseGI	GGATG	4 cut(s)	475, 1176, 1617, 1741
BseJI	GATNNNNATC	1 cut(s)	2208
BseLI	CCNNNNNNNGG	7 cut(s)	123, 573, 627, 931, 1091, 1861, 1910
BseMII	CTCAG	3 cut(s)	434, 447, 685
BseNI	ACTGG	4 cut(s)	143, 1582, 2085, 2221
BseRI	GAGGAG	3 cut(s)	25, 473, 1648
BseX3I	CGGCCG	1 cut(s)	1950
BseXI	GCAGC	3 cut(s)	449, 1312, 1315
BseYI	CCCAGC	1 cut(s)	122
Bsh1236I	CGCG	2 cut(s)	1080, 2138
Bsh1285I	CGRYCG	1 cut(s)	1953
BshFI	GGCC	3 cut(s)	539, 566, 1952
BshVI	ATCGAT	2 cut(s)	1149, 2181
BsiEI	CGRYCG	1 cut(s)	1953
BsiHKCI	CYCGRG	2 cut(s)	1039, 2165
BsiSI	CCGG	3 cut(s)	467, 1455, 1949
BslFI	GGGAC	2 cut(s)	328, 1056
BslI	CCNNNNNNNGG	7 cut(s)	123, 573, 627, 931, 1091, 1861, 1910
BsmAI	GTCTC	4 cut(s)	252, 907, 1192, 1973
BsmFI	GGGAC	2 cut(s)	328, 1056
BsmI	GAATGC	3 cut(s)	159, 1525, 1937
BsnI	GGCC	3 cut(s)	539, 566, 1952
Bso31I	GGTCTC	1 cut(s)	1192
BsoBI	CYCGRG	2 cut(s)	1039, 2165
Bsp119I	TTCGAA	1 cut(s)	1713
Bsp143I	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
Bsp1720I	GCTNAGC	1 cut(s)	1356
BspACI	CCGC	6 cut(s)	149, 522, 675, 1049, 1550, 2136
BspANI	GGCC	3 cut(s)	539, 566, 1952
BspCNI	CTCAG	3 cut(s)	435, 448, 684
BspDI	ATCGAT	2 cut(s)	1149, 2181
BspFNI	CGCG	2 cut(s)	1080, 2138
BspHI	TCATGA	2 cut(s)	1401, 1683
BspLI	GGNNCC	3 cut(s)	344, 465, 1044
BspPI	GGATC	2 cut(s)	65, 2178
BspT104I	TTCGAA	1 cut(s)	1713
BspTNI	GGTCTC	1 cut(s)	1192
BsrFI	RCCGGY	1 cut(s)	1948
BsrI	ACTGG	4 cut(s)	143, 1582, 2085, 2221
BssAI	RCCGGY	1 cut(s)	1948
BssECI	CCNNGG	1 cut(s)	1221
BssMI	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
BssNAI	GTATAC	1 cut(s)	1501
BssNI	GRCGYC	2 cut(s)	1338, 1752
Bst1107I	GTATAC	1 cut(s)	1501
Bst4CI	ACNGT	5 cut(s)	300, 357, 1591, 1778, 1819
Bst6I	CTCTTC	3 cut(s)	912, 1053, 2114
BstACI	GRCGYC	2 cut(s)	1338, 1752
BstBI	TTCGAA	1 cut(s)	1713
BstC8I	GCNNGC	1 cut(s)	1082
BstDEI	CTNAG	8 cut(s)	6, 276, 347, 443, 456, 671, 1100, 1356
BstDSI	CCRYGG	1 cut(s)	1221
BstEII	GGTNACC	1 cut(s)	1094
BstF5I	GGATG	4 cut(s)	475, 1176, 1617, 1741
BstFNI	CGCG	2 cut(s)	1080, 2138
BstHHI	GCGC	2 cut(s)	1080, 2140
BstKTI	GATC	9 cut(s)	73, 1149, 1215, 1689, 1762, 1807, 2145, 2173, 2181
BstMAI	GTCTC	4 cut(s)	252, 907, 1192, 1973
BstMBI	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
BstMCI	CGRYCG	1 cut(s)	1953
BstMWI	GCNNNNNNNGC	3 cut(s)	437, 1086, 1325
BstNSI	RCATGY	1 cut(s)	682
BstPAI	GACNNNNGTC	1 cut(s)	899
BstPI	GGTNACC	1 cut(s)	1094
BstSFI	CTRYAG	2 cut(s)	1518, 1894
BstUI	CGCG	2 cut(s)	1080, 2138
BstV1I	GCAGC	3 cut(s)	449, 1312, 1315
BstV2I	GAAGAC	4 cut(s)	784, 944, 1333, 1645
BstX2I	RGATCY	3 cut(s)	70, 1804, 2170
BstXI	CCANNNNNNTGG	1 cut(s)	604
BstYI	RGATCY	3 cut(s)	70, 1804, 2170
BstZ17I	GTATAC	1 cut(s)	1501
BstZI	CGGCCG	1 cut(s)	1950
Bsu15I	ATCGAT	2 cut(s)	1149, 2181
Bsu36I	CCTNAGG	1 cut(s)	347
BsuI	GTATCC	1 cut(s)	2028
BsuRI	GGCC	3 cut(s)	539, 566, 1952
BsuTUI	ATCGAT	2 cut(s)	1149, 2181
BtgI	CCRYGG	1 cut(s)	1221
BtgZI	GCGATG	2 cut(s)	1918, 2131
BtsCI	GGATG	4 cut(s)	475, 1176, 1617, 1741
BtsIMutI	CAGTG	4 cut(s)	150, 440, 1529, 2214
Cac8I	GCNNGC	1 cut(s)	1082
CciI	TCATGA	2 cut(s)	1401, 1683
CfoI	GCGC	2 cut(s)	1080, 2140
Cfr10I	RCCGGY	1 cut(s)	1948
Cfr13I	GGNCC	3 cut(s)	342, 1043, 2083
ClaI	ATCGAT	2 cut(s)	1149, 2181
CseI	GACGC	1 cut(s)	420
Csp6I	GTAC	5 cut(s)	757, 855, 1427, 1616, 1695
CviAII	CATG	5 cut(s)	679, 700, 767, 1402, 1684
CviQI	GTAC	5 cut(s)	757, 855, 1427, 1616, 1695
DdeI	CTNAG	8 cut(s)	6, 276, 347, 443, 456, 671, 1100, 1356
DpnI	GATC	9 cut(s)	72, 1148, 1214, 1688, 1761, 1806, 2144, 2172, 2180
DpnII	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
DraIII	CACNNNGTG	1 cut(s)	511
EaeI	YGGCCR	2 cut(s)	564, 1950
EagI	CGGCCG	1 cut(s)	1950
Eam1104I	CTCTTC	3 cut(s)	912, 1053, 2114
EarI	CTCTTC	3 cut(s)	912, 1053, 2114
EclXI	CGGCCG	1 cut(s)	1950
Eco31I	GGTCTC	1 cut(s)	1192
Eco47I	GGWCC	3 cut(s)	342, 1043, 2083
Eco52I	CGGCCG	1 cut(s)	1950
Eco57I	CTGAAG	3 cut(s)	246, 1013, 1215
Eco81I	CCTNAGG	1 cut(s)	347
Eco88I	CYCGRG	2 cut(s)	1039, 2165
Eco91I	GGTNACC	1 cut(s)	1094
EcoO109I	RGGNCCY	2 cut(s)	342, 1043
EcoO65I	GGTNACC	1 cut(s)	1094
EcoRI	GAATTC	1 cut(s)	2160
EcoT22I	ATGCAT	2 cut(s)	705, 1743
FaeI	CATG	5 cut(s)	682, 703, 770, 1405, 1687
FalI	AAGNNNNNCTT	6 cut(s)	370, 402, 1407, 1439, 1694, 1726
FaqI	GGGAC	2 cut(s)	328, 1056
FatI	CATG	5 cut(s)	678, 699, 766, 1401, 1683
FbaI	TGATCA	1 cut(s)	2142
FblI	GTMKAC	4 cut(s)	1419, 1500, 1773, 1923
Fnu4HI	GCNGC	5 cut(s)	149, 438, 676, 1326, 1329
FokI	GGATG	4 cut(s)	482, 1183, 1624, 1728
Fsp4HI	GCNGC	5 cut(s)	149, 438, 676, 1326, 1329
FspBI	CTAG	4 cut(s)	233, 321, 878, 1259
GlaI	GCGC	2 cut(s)	1079, 2139
GluI	GCNGC	5 cut(s)	149, 438, 676, 1326, 1329
GsaI	CCCAGC	1 cut(s)	126
HaeIII	GGCC	3 cut(s)	539, 566, 1952
HapII	CCGG	3 cut(s)	467, 1455, 1949
HgaI	GACGC	1 cut(s)	420
HhaI	GCGC	2 cut(s)	1080, 2140
Hin1I	GRCGYC	2 cut(s)	1338, 1752
Hin1II	CATG	5 cut(s)	682, 703, 770, 1405, 1687
Hin6I	GCGC	2 cut(s)	1078, 2138
HinP1I	GCGC	2 cut(s)	1078, 2138
HincII	GTYRAC	3 cut(s)	1270, 1774, 1924
HindII	GTYRAC	3 cut(s)	1270, 1774, 1924
HindIII	AAGCTT	1 cut(s)	1640
HinfI	GANTC	8 cut(s)	15, 62, 113, 997, 1004, 1033, 1581, 1715
HpaII	CCGG	3 cut(s)	467, 1455, 1949
HphI	GGTGA	9 cut(s)	99, 619, 697, 854, 946, 1088, 1141, 2044, 2050
Hpy166II	GTNNAC	7 cut(s)	610, 1165, 1270, 1420, 1501, 1774, 1924
Hpy8I	GTNNAC	7 cut(s)	610, 1165, 1270, 1420, 1501, 1774, 1924
Hpy99I	CGWCG	2 cut(s)	1757, 1778
HpyAV	CCTTC	5 cut(s)	96, 221, 333, 1177, 2204
HpyCH4III	ACNGT	5 cut(s)	300, 357, 1591, 1778, 1819
HpyCH4IV	ACGT	7 cut(s)	853, 941, 975, 1023, 1071, 1338, 1752
HpyCH4V	TGCA	4 cut(s)	377, 703, 766, 1741
HpyF10VI	GCNNNNNNNGC	3 cut(s)	437, 1086, 1325
HpyF3I	CTNAG	8 cut(s)	6, 276, 347, 443, 456, 671, 1100, 1356
HpySE526I	ACGT	7 cut(s)	853, 941, 975, 1023, 1071, 1338, 1752
Hsp92I	GRCGYC	2 cut(s)	1338, 1752
Hsp92II	CATG	5 cut(s)	682, 703, 770, 1405, 1687
HspAI	GCGC	2 cut(s)	1078, 2138
Ksp22I	TGATCA	1 cut(s)	2142
Kzo9I	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
LmnI	GCTCC	2 cut(s)	292, 423
Lsp1109I	GCAGC	3 cut(s)	449, 1312, 1315
LweI	GCATC	7 cut(s)	89, 109, 1435, 1640, 1728, 1750, 1941
MaeI	CTAG	4 cut(s)	233, 321, 878, 1259
MaeII	ACGT	7 cut(s)	853, 941, 975, 1023, 1071, 1338, 1752
MaeIII	GTNAC	5 cut(s)	20, 664, 685, 1094, 2219
MalI	GATC	9 cut(s)	72, 1148, 1214, 1688, 1761, 1806, 2144, 2172, 2180
MboI	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
MfeI	CAATTG	1 cut(s)	2046
MflI	RGATCY	3 cut(s)	70, 1804, 2170
MlsI	TGGCCA	1 cut(s)	566
MluNI	TGGCCA	1 cut(s)	566
MlyI	GAGTC	4 cut(s)	24, 107, 1013, 1027
MmeI	TCCRAC	5 cut(s)	612, 1490, 1542, 1735, 2110
Mox20I	TGGCCA	1 cut(s)	566
Mph1103I	ATGCAT	2 cut(s)	705, 1743
MroXI	GAANNNNTTC	2 cut(s)	1709, 1847
MscI	TGGCCA	1 cut(s)	566
MslI	CAYNNNNRTG	3 cut(s)	602, 683, 1746
Msp20I	TGGCCA	1 cut(s)	566
MspA1I	CMGCKG	2 cut(s)	675, 1550
MspI	CCGG	3 cut(s)	467, 1455, 1949
MunI	CAATTG	1 cut(s)	2046
Mva1269I	GAATGC	3 cut(s)	159, 1525, 1937
MvnI	CGCG	2 cut(s)	1080, 2138
MwoI	GCNNNNNNNGC	3 cut(s)	437, 1086, 1325
NdeII	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
NlaIII	CATG	5 cut(s)	682, 703, 770, 1405, 1687
NlaIV	GGNNCC	3 cut(s)	344, 465, 1044
NmeAIII	GCCGAG	1 cut(s)	1978
NmuCI	GTSAC	3 cut(s)	685, 1094, 2219
NsiI	ATGCAT	2 cut(s)	705, 1743
NspI	RCATGY	1 cut(s)	682
NspV	TTCGAA	1 cut(s)	1713
PaeR7I	CTCGAG	1 cut(s)	2165
PagI	TCATGA	2 cut(s)	1401, 1683
PcsI	WCGNNNNNNNCGW	1 cut(s)	1077
PctI	GAATGC	3 cut(s)	159, 1525, 1937
PdmI	GAANNNNTTC	2 cut(s)	1709, 1847
PfeI	GAWTC	4 cut(s)	62, 997, 1581, 1715
PflFI	GACNNNGTC	1 cut(s)	1265
PkrI	GCNGC	5 cut(s)	150, 439, 677, 1327, 1330
PleI	GAGTC	4 cut(s)	23, 107, 1012, 1027
PpsI	GAGTC	4 cut(s)	23, 107, 1012, 1027
PpuMI	RGGWCCY	2 cut(s)	342, 1043
PshAI	GACNNNNGTC	1 cut(s)	899
PshBI	ATTAAT	2 cut(s)	42, 1436
PsiI	TTATAA	1 cut(s)	1574
Psp1406I	AACGTT	1 cut(s)	1071
Psp5II	RGGWCCY	2 cut(s)	342, 1043
PspEI	GGTNACC	1 cut(s)	1094
PspFI	CCCAGC	1 cut(s)	122
PspN4I	GGNNCC	3 cut(s)	344, 465, 1044
PspPI	GGNCC	3 cut(s)	342, 1043, 2083
PspPPI	RGGWCCY	2 cut(s)	342, 1043
PsuI	RGATCY	3 cut(s)	70, 1804, 2170
PsyI	GACNNNGTC	1 cut(s)	1265
RsaI	GTAC	5 cut(s)	758, 856, 1428, 1617, 1696
RsaNI	GTAC	5 cut(s)	757, 855, 1427, 1616, 1695
RseI	CAYNNNNRTG	3 cut(s)	602, 683, 1746
SalI	GTCGAC	2 cut(s)	1772, 1922
SatI	GCNGC	5 cut(s)	149, 438, 676, 1326, 1329
Sau3AI	GATC	9 cut(s)	70, 1146, 1212, 1686, 1759, 1804, 2142, 2170, 2178
Sau96I	GGNCC	3 cut(s)	342, 1043, 2083
ScaI	AGTACT	1 cut(s)	1696
SchI	GAGTC	4 cut(s)	24, 107, 1013, 1027
SfaNI	GCATC	7 cut(s)	89, 109, 1435, 1640, 1728, 1750, 1941
SfcI	CTRYAG	2 cut(s)	1518, 1894
Sfr274I	CTCGAG	1 cut(s)	2165
SfuI	TTCGAA	1 cut(s)	1713
SinI	GGWCC	3 cut(s)	342, 1043, 2083
SlaI	CTCGAG	1 cut(s)	2165
SmiMI	CAYNNNNRTG	3 cut(s)	602, 683, 1746
SmlI	CTYRAG	6 cut(s)	545, 1826, 1970, 2165, 2174, 2199
SmoI	CTYRAG	6 cut(s)	545, 1826, 1970, 2165, 2174, 2199
SsiI	CCGC	6 cut(s)	149, 522, 675, 1049, 1550, 2136
SspMI	CTAG	4 cut(s)	233, 321, 878, 1259
TaaI	ACNGT	5 cut(s)	300, 357, 1591, 1778, 1819
TaiI	ACGT	7 cut(s)	856, 944, 978, 1026, 1074, 1341, 1755
TatI	WGTACW	3 cut(s)	756, 1426, 1694
TauI	GCSGC	2 cut(s)	151, 678
TfiI	GAWTC	4 cut(s)	62, 997, 1581, 1715
TscAI	CASTG	4 cut(s)	150, 447, 1536, 2221
TseFI	GTSAC	3 cut(s)	685, 1094, 2219
TseI	GCWGC	3 cut(s)	437, 1325, 1328
Tsp45I	GTSAC	3 cut(s)	685, 1094, 2219
TspGWI	ACGGA	1 cut(s)	39
TspRI	CASTG	4 cut(s)	150, 447, 1536, 2221
Tth111I	GACNNNGTC	1 cut(s)	1265
VpaK11BI	GGWCC	3 cut(s)	342, 1043, 2083
VspI	ATTAAT	2 cut(s)	42, 1436
XapI	RAATTY	5 cut(s)	213, 635, 1858, 2098, 2160
XbaI	TCTAGA	2 cut(s)	877, 1258
XceI	RCATGY	1 cut(s)	682
XcmI	CCANNNNNNNNNTGG	2 cut(s)	628, 1645
XhoI	CTCGAG	1 cut(s)	2165
XmiI	GTMKAC	4 cut(s)	1419, 1500, 1773, 1923
XmnI	GAANNNNTTC	2 cut(s)	1709, 1847
XspI	CTAG	4 cut(s)	233, 321, 878, 1259
ZraI	GACGTC	2 cut(s)	1339, 1753
ZrmI	AGTACT	1 cut(s)	1696
Zsp2I	ATGCAT	2 cut(s)	705, 1743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.