Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

54247397 .. 54250069

Gene Structure 2673 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00323490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGGACTCTCCCAACAGATTGCCAGCTGAAATTTCCAATGAGACTCAAACTACCAGAAAGCCATTCAATCGGTCACTTATCTCCAAACCCAAGTCCAGAAAGTCGTTCAACAGGTCAGTTCTCTCCAAGCCCAAGTCCAGATTTGGGGAACCGTCAGTGCCCATTGACTCTGATTTGTTTGAAGAGAGCAATAGTGTGGAAACTTCACCTCCTAATAGCAATATGGGTTCATTCAATAGGGCCAATAATAATTCCCCTGATTTTAGAACTGTCTCCATCAGCTCTGCATGGAACCCAGTAATGGCGTCTCCAGGCAGGGCCAAAGCCGAAGAGGAGGACAAAAAGTTTTACAAGGTGATCAGGGTATTGATTGAGTGGACTGTTTTCCTTTGCATTTTGGGTGTTTTAGTGGCTAGCTTGACTGTCGAGAAACTCGAGAAGCTTAAGGTTTGGGGTTTGGAGTTTTGGAAATGGTGTGTGCTTCTGATGGCAGTATTCGGTGGCACGCTGCTCACATATTGGTTCACGCATTTCGTAGTCCTTTTCACCAAGAGGAACATACTGCTCAGGAAAAAGCTGTTGTACTATGTTTATGGAATGAAGAGGAGTGTTCAGGTTTTCATGTGGTTGGCTTTGATTCTTCTAACGTGGTTACTATTGTTCAATCCTAGTCGTCATGAGATTAAGCGATCAAAGACTCGTACCAAGATTTTGAAATATGTTACTTGGACTCTTGCTTCGGCTGTTATTGGGGCATTTCTGTGGTTGTTGAAAACTCTGTTGTTTAAAATCGTAGCACTTAAGTTCCATCTGCGCCACACCTACTTTGATAGGGTTCAAGAGTCGATCTTCCATCAGTACATTCTTCAGACACTTTCAGGGCATCCAGTTATAAGAGGAAGCCAAGAGGATCGGGAGATCACCGAGCACTGGCCAACTGAGTATCATAAGCACCAGGAAGGGCTATCCACTTTCTCTTATACGTTGGATCGAGACAGAGGACTTAAACAGAAAGAAATGCAGATTGTCAATGAGATTCAAGCAATTGCTGCTGCCTCCCATATTTTTACCAATGTTGCTCAGCGTGATTACGAGTACATTGAGGAGGACGACCTCAGGAGGTTCATGATTAATGAAGACGTGGAACTTGTGTTTCGATTGATTGATGTCGGAGAGACGGGAAAAATTGACAAAAGAGCTCTAACAGATTGGGTGGTCAAGGTCTACAATGGTCGCAAAGCATTATATCATGCTCTAACCGACACCAAAGCAGCTGTAAAGCAATTGAACGAAATTGTGACCGGCATCCTGATTGTTATTATCATTGTTGTGTGGCTTATATTAATGGAAGTTGCAACCACGAAAGTACTTGTTTTCATCTCATCACAGCTCGTATTAGCAGCCTTTATCTTTGGAAACACTTGCAAGAACATATTTGAAGCTATCGTATTTGTCTTTGTAATACATCCATTTGATGTTGGTGACCGCTGTCTTGTTGATGGCGTTCCGCTGAGGGTTGAGGAGATGAACATCCTTACTACAGTTTTCTTGAAACTAAATAATGAGAGGGTATACTATCCAAACTCAGTTTTATCAACAAAGACCATTAGCAATTATTATAGAAGTTCAGACATGGGAGATACCGTGGATTTCTCAATCGATTTAATTCCAGTTGACAGGATTAACCTGCTGAAAGCGAAAATAAAGAGTTATTTGGAGAGTAACCCGCAGTATTGGCACCCAGATCACAATGTGGTGGTGATTGAGATTGAAAATGTGAATAAGTTAAAGATGGCACTCTATTTTAATCACACTATGAACTTTCAAGAATTTAGGGAAATAAACAAACGGAGATCTGAGCTGGTGATTGAATTGATGAGAATATTTGAAGAGCTCAACATCGCATACAATCTGCTTCCTCACGATGTCAGCGTGGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

646

Amino Acids

75.06

Weight (kDa)

9.12

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	115 - 269	9.8e-07	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	475 - 540	9.7e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	893
Acc36I	ACCTGC	1 cut(s)	1695
AccB1I	GGYRCC	1 cut(s)	1737
AccI	GTMKAC	2 cut(s)	1224, 1572
AciI	CCGC	3 cut(s)	1486, 1508, 1727
AclWI	GGATC	2 cut(s)	918, 996
AcoI	YGGCCR	1 cut(s)	932
AcsI	RAATTY	2 cut(s)	30, 1829
AcuI	CTGAAG	1 cut(s)	851
AcyI	GRCGYC	1 cut(s)	305
AdeI	CACNNNGTG	1 cut(s)	1753
AfaI	GTAC	5 cut(s)	584, 703, 860, 1097, 1368
AfiI	CCNNNNNNNGG	3 cut(s)	144, 301, 930
AflII	CTTAAG	2 cut(s)	443, 800
AjiI	CACGTC	1 cut(s)	1141
AjnI	CCWGG	2 cut(s)	310, 954
Alw21I	GWGCWC	3 cut(s)	930, 1201, 1896
Alw26I	GTCTC	5 cut(s)	35, 277, 312, 987, 1169
AlwI	GGATC	2 cut(s)	918, 996
Ama87I	CYCGRG	1 cut(s)	434
AoxI	GGCC	4 cut(s)	240, 318, 932, 1935
ApeKI	GCWGC	5 cut(s)	508, 1049, 1052, 1271, 1400
ApoI	RAATTY	2 cut(s)	30, 1829
ArsI	GACNNNNNNTTYG	2 cut(s)	721, 753
AseI	ATTAAT	2 cut(s)	1131, 1343
AspLEI	GCGC	1 cut(s)	816
AspS9I	GGNCC	2 cut(s)	240, 318
AsuHPI	GGTGA	7 cut(s)	198, 367, 538, 913, 1493, 1771, 1876
AsuNHI	GCTAGC	1 cut(s)	413
AvaI	CYCGRG	1 cut(s)	434
AxyI	CCTNAGG	1 cut(s)	1115
BaeGI	GKGCMC	1 cut(s)	162
BalI	TGGCCA	1 cut(s)	934
BanI	GGYRCC	1 cut(s)	1737
BanII	GRGCYC	2 cut(s)	1201, 1896
BbsI	GAAGAC	1 cut(s)	1143
Bbv12I	GWGCWC	3 cut(s)	930, 1201, 1896
BbvCI	CCTCAGC	1 cut(s)	1511
BbvI	GCAGC	5 cut(s)	495, 1036, 1039, 1283, 1412
BccI	CCATC	6 cut(s)	284, 481, 816, 861, 1493, 1786
BciT130I	CCWGG	2 cut(s)	312, 956
BclI	TGATCA	1 cut(s)	357
BcoDI	GTCTC	5 cut(s)	35, 277, 312, 987, 1169
BfaI	CTAG	2 cut(s)	414, 669
BfmI	CTRYAG	1 cut(s)	1539
BfrI	CTTAAG	2 cut(s)	443, 800
BfuAI	ACCTGC	1 cut(s)	1695
BglII	AGATCT	1 cut(s)	1853
BisI	GCNGC	5 cut(s)	509, 1050, 1053, 1272, 1401
BlpI	GCTNAGC	1 cut(s)	1080
BlsI	GCNGC	5 cut(s)	510, 1051, 1054, 1273, 1402
BmcAI	AGTACT	1 cut(s)	1368
Bme1390I	CCNGG	2 cut(s)	312, 956
BmeT110I	CYCGRG	1 cut(s)	434
BmgBI	CACGTC	1 cut(s)	1141
BmgT120I	GGNCC	2 cut(s)	240, 318
BmiI	GGNNCC	3 cut(s)	150, 293, 1739
BmrFI	CCNGG	2 cut(s)	312, 956
BmrI	ACTGGG	1 cut(s)	290
BmsI	GCATC	2 cut(s)	892, 1314
BmtI	GCTAGC	1 cut(s)	417
BmuI	ACTGGG	1 cut(s)	290
BoxI	GACNNNNGTC	1 cut(s)	1488
BpiI	GAAGAC	1 cut(s)	1143
BpmI	CTGGAG	1 cut(s)	294
Bpu10I	CCTNAGC	2 cut(s)	566, 1511
Bpu1102I	GCTNAGC	1 cut(s)	1080
Bsa29I	ATCGAT	1 cut(s)	1659
BsaBI	GATNNNNATC	1 cut(s)	1529
BsaHI	GRCGYC	1 cut(s)	305
BsaJI	CCNNGG	1 cut(s)	1644
BsaXI	ACNNNNNCTCC	4 cut(s)	193, 223, 1629, 1659
Bsc4I	CCNNNNNNNGG	3 cut(s)	144, 301, 930
Bse118I	RCCGGY	1 cut(s)	1301
Bse1I	ACTGG	4 cut(s)	296, 887, 935, 1670
Bse21I	CCTNAGG	1 cut(s)	1115
Bse8I	GATNNNNATC	1 cut(s)	1529
BseBI	CCWGG	2 cut(s)	312, 956
BseCI	ATCGAT	1 cut(s)	1659
BseDI	CCNNGG	1 cut(s)	1644
BseGI	GGATG	4 cut(s)	883, 1305, 1465, 1530
BseJI	GATNNNNATC	1 cut(s)	1529
BseLI	CCNNNNNNNGG	3 cut(s)	144, 301, 930
BseMII	CTCAG	7 cut(s)	580, 930, 1094, 1129, 1502, 1599, 1848
BseNI	ACTGG	4 cut(s)	296, 887, 935, 1670
BseRI	GAGGAG	4 cut(s)	347, 619, 1118, 1535
BseSI	GKGCMC	1 cut(s)	162
BseXI	GCAGC	5 cut(s)	495, 1036, 1039, 1283, 1412
BshFI	GGCC	4 cut(s)	242, 320, 934, 1937
BshNI	GGYRCC	1 cut(s)	1737
BshVI	ATCGAT	1 cut(s)	1659
BsiHKAI	GWGCWC	3 cut(s)	930, 1201, 1896
BsiHKCI	CYCGRG	1 cut(s)	434
BsiSI	CCGG	1 cut(s)	1302
BslI	CCNNNNNNNGG	3 cut(s)	144, 301, 930
BsmAI	GTCTC	5 cut(s)	35, 277, 312, 987, 1169
BsmBI	CGTCTC	2 cut(s)	312, 1169
BsnI	GGCC	4 cut(s)	242, 320, 934, 1937
BsoBI	CYCGRG	1 cut(s)	434
Bsp1286I	GDGCHC	4 cut(s)	162, 930, 1201, 1896
Bsp143I	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
Bsp1720I	GCTNAGC	1 cut(s)	1080
BspACI	CCGC	3 cut(s)	1486, 1508, 1727
BspANI	GGCC	4 cut(s)	242, 320, 934, 1937
BspCNI	CTCAG	7 cut(s)	579, 931, 1093, 1128, 1503, 1598, 1849
BspDI	ATCGAT	1 cut(s)	1659
BspHI	TCATGA	2 cut(s)	676, 1125
BspLI	GGNNCC	3 cut(s)	150, 293, 1739
BspMI	ACCTGC	1 cut(s)	1695
BspOI	GCTAGC	1 cut(s)	417
BspPI	GGATC	2 cut(s)	918, 996
BspQI	GCTCTTC	1 cut(s)	1884
BspT107I	GGYRCC	1 cut(s)	1737
BspTI	CTTAAG	2 cut(s)	443, 800
BsrFI	RCCGGY	1 cut(s)	1301
BsrI	ACTGG	4 cut(s)	296, 887, 935, 1670
BssAI	RCCGGY	1 cut(s)	1301
BssECI	CCNNGG	1 cut(s)	1644
BssMI	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
BssNAI	GTATAC	1 cut(s)	1573
BssNI	GRCGYC	1 cut(s)	305
Bst1107I	GTATAC	1 cut(s)	1573
Bst2UI	CCWGG	2 cut(s)	312, 956
Bst4CI	ACNGT	6 cut(s)	153, 271, 382, 424, 1543, 1645
Bst6I	CTCTTC	4 cut(s)	177, 324, 596, 1884
BstACI	GRCGYC	1 cut(s)	305
BstAFI	CTTAAG	2 cut(s)	443, 800
BstAPI	GCANNNNNTGC	1 cut(s)	1049
BstC8I	GCNNGC	3 cut(s)	24, 415, 506
BstDEI	CTNAG	7 cut(s)	566, 939, 1080, 1115, 1511, 1585, 1857
BstDSI	CCRYGG	1 cut(s)	1644
BstEII	GGTNACC	1 cut(s)	1481
BstF5I	GGATG	4 cut(s)	883, 1305, 1465, 1530
BstHHI	GCGC	1 cut(s)	816
BstKTI	GATC	8 cut(s)	360, 692, 849, 913, 921, 991, 1747, 1856
BstMAI	GTCTC	5 cut(s)	35, 277, 312, 987, 1169
BstMBI	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
BstMWI	GCNNNNNNNGC	2 cut(s)	1049, 1735
BstNI	CCWGG	2 cut(s)	312, 956
BstPAI	GACNNNNGTC	1 cut(s)	1488
BstPI	GGTNACC	1 cut(s)	1481
BstSCI	CCNGG	2 cut(s)	310, 954
BstSFI	CTRYAG	1 cut(s)	1539
BstSLI	GKGCMC	1 cut(s)	162
BstV1I	GCAGC	5 cut(s)	495, 1036, 1039, 1283, 1412
BstV2I	GAAGAC	1 cut(s)	1143
BstX2I	RGATCY	1 cut(s)	1853
BstYI	RGATCY	1 cut(s)	1853
BstZ17I	GTATAC	1 cut(s)	1573
Bsu15I	ATCGAT	1 cut(s)	1659
Bsu36I	CCTNAGG	1 cut(s)	1115
BsuRI	GGCC	4 cut(s)	242, 320, 934, 1937
BsuTUI	ATCGAT	1 cut(s)	1659
BtgI	CCRYGG	1 cut(s)	1644
BtgZI	GCGATG	1 cut(s)	1885
BtrI	CACGTC	1 cut(s)	1141
BtsCI	GGATG	4 cut(s)	883, 1305, 1465, 1530
BtsIMutI	CAGTG	2 cut(s)	162, 928
BveI	ACCTGC	1 cut(s)	1695
Cac8I	GCNNGC	3 cut(s)	24, 415, 506
CciI	TCATGA	2 cut(s)	676, 1125
CfoI	GCGC	1 cut(s)	816
Cfr10I	RCCGGY	1 cut(s)	1301
Cfr13I	GGNCC	2 cut(s)	240, 318
ClaI	ATCGAT	1 cut(s)	1659
CseI	GACGC	1 cut(s)	294
Csp6I	GTAC	5 cut(s)	583, 702, 859, 1096, 1367
CviAII	CATG	6 cut(s)	288, 622, 677, 1126, 1250, 1633
CviQI	GTAC	5 cut(s)	583, 702, 859, 1096, 1367
DdeI	CTNAG	7 cut(s)	566, 939, 1080, 1115, 1511, 1585, 1857
DpnI	GATC	8 cut(s)	359, 691, 848, 912, 920, 990, 1746, 1855
DpnII	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
DraI	TTTAAA	1 cut(s)	787
DraIII	CACNNNGTG	1 cut(s)	1753
EaeI	YGGCCR	1 cut(s)	932
Eam1104I	CTCTTC	4 cut(s)	177, 324, 596, 1884
EarI	CTCTTC	4 cut(s)	177, 324, 596, 1884
Ecl136II	GAGCTC	2 cut(s)	1199, 1894
Eco24I	GRGCYC	2 cut(s)	1201, 1896
Eco53kI	GAGCTC	2 cut(s)	1199, 1894
Eco57I	CTGAAG	1 cut(s)	851
Eco81I	CCTNAGG	1 cut(s)	1115
Eco88I	CYCGRG	1 cut(s)	434
Eco91I	GGTNACC	1 cut(s)	1481
EcoICRI	GAGCTC	2 cut(s)	1199, 1894
EcoO65I	GGTNACC	1 cut(s)	1481
EcoRII	CCWGG	2 cut(s)	310, 954
EcoT38I	GRGCYC	2 cut(s)	1201, 1896
Esp3I	CGTCTC	2 cut(s)	312, 1169
FaeI	CATG	6 cut(s)	291, 625, 680, 1129, 1253, 1636
FatI	CATG	6 cut(s)	287, 621, 676, 1125, 1249, 1632
FauI	CCCGC	1 cut(s)	1734
FbaI	TGATCA	1 cut(s)	357
FblI	GTMKAC	2 cut(s)	1224, 1572
Fnu4HI	GCNGC	5 cut(s)	509, 1050, 1053, 1272, 1401
FokI	GGATG	4 cut(s)	870, 1292, 1452, 1517
FriOI	GRGCYC	2 cut(s)	1201, 1896
Fsp4HI	GCNGC	5 cut(s)	509, 1050, 1053, 1272, 1401
FspBI	CTAG	2 cut(s)	414, 669
GlaI	GCGC	1 cut(s)	815
GluI	GCNGC	5 cut(s)	509, 1050, 1053, 1272, 1401
GsuI	CTGGAG	1 cut(s)	294
HaeIII	GGCC	4 cut(s)	242, 320, 934, 1937
HapII	CCGG	1 cut(s)	1302
HgaI	GACGC	1 cut(s)	294
HhaI	GCGC	1 cut(s)	816
Hin1I	GRCGYC	1 cut(s)	305
Hin1II	CATG	6 cut(s)	291, 625, 680, 1129, 1253, 1636
Hin6I	GCGC	1 cut(s)	814
HinP1I	GCGC	1 cut(s)	814
HincII	GTYRAC	1 cut(s)	1675
HindII	GTYRAC	1 cut(s)	1675
HindIII	AAGCTT	1 cut(s)	440
HinfI	GANTC	8 cut(s)	5, 43, 167, 637, 697, 730, 842, 1036
HpaII	CCGG	1 cut(s)	1302
HphI	GGTGA	7 cut(s)	198, 367, 538, 913, 1493, 1771, 1876
Hpy166II	GTNNAC	5 cut(s)	378, 525, 1225, 1573, 1675
Hpy188I	TCNGA	6 cut(s)	172, 486, 870, 1172, 1630, 1858
Hpy8I	GTNNAC	5 cut(s)	378, 525, 1225, 1573, 1675
HpyAV	CCTTC	1 cut(s)	953
HpyCH4III	ACNGT	6 cut(s)	153, 271, 382, 424, 1543, 1645
HpyCH4IV	ACGT	3 cut(s)	647, 983, 1140
HpyCH4V	TGCA	5 cut(s)	287, 393, 1021, 1355, 1425
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1049, 1735
HpyF3I	CTNAG	7 cut(s)	566, 939, 1080, 1115, 1511, 1585, 1857
HpySE526I	ACGT	3 cut(s)	647, 983, 1140
Hsp92I	GRCGYC	1 cut(s)	305
Hsp92II	CATG	6 cut(s)	291, 625, 680, 1129, 1253, 1636
HspAI	GCGC	1 cut(s)	814
Ksp22I	TGATCA	1 cut(s)	357
Kzo9I	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
LguI	GCTCTTC	1 cut(s)	1884
Lsp1109I	GCAGC	5 cut(s)	495, 1036, 1039, 1283, 1412
LweI	GCATC	2 cut(s)	892, 1314
MaeI	CTAG	2 cut(s)	414, 669
MaeII	ACGT	3 cut(s)	647, 983, 1140
MaeIII	GTNAC	6 cut(s)	72, 651, 721, 1297, 1481, 1721
MalI	GATC	8 cut(s)	359, 691, 848, 912, 920, 990, 1746, 1855
MboI	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
MboII	GAAGA	8 cut(s)	194, 341, 613, 632, 841, 857, 1148, 1901
MfeI	CAATTG	2 cut(s)	1044, 1283
MflI	RGATCY	1 cut(s)	1853
MhlI	GDGCHC	4 cut(s)	162, 930, 1201, 1896
MlsI	TGGCCA	1 cut(s)	934
MluNI	TGGCCA	1 cut(s)	934
MlyI	GAGTC	5 cut(s)	37, 161, 691, 724, 851
MmeI	TCCRAC	2 cut(s)	966, 1150
Mox20I	TGGCCA	1 cut(s)	934
MscI	TGGCCA	1 cut(s)	934
MslI	CAYNNNNRTG	2 cut(s)	760, 1328
Msp20I	TGGCCA	1 cut(s)	934
MspA1I	CMGCKG	4 cut(s)	26, 1274, 1488, 1510
MspCI	CTTAAG	2 cut(s)	443, 800
MspI	CCGG	1 cut(s)	1302
MspR9I	CCNGG	2 cut(s)	312, 956
MunI	CAATTG	2 cut(s)	1044, 1283
MvaI	CCWGG	2 cut(s)	312, 956
MwoI	GCNNNNNNNGC	2 cut(s)	1049, 1735
NdeII	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
NheI	GCTAGC	1 cut(s)	413
NlaIII	CATG	6 cut(s)	291, 625, 680, 1129, 1253, 1636
NlaIV	GGNNCC	3 cut(s)	150, 293, 1739
NmuCI	GTSAC	3 cut(s)	72, 1297, 1481
PaeR7I	CTCGAG	1 cut(s)	434
PagI	TCATGA	2 cut(s)	676, 1125
PciSI	GCTCTTC	1 cut(s)	1884
PcsI	WCGNNNNNNNCGW	2 cut(s)	432, 1929
PfeI	GAWTC	2 cut(s)	637, 1036
PkrI	GCNGC	5 cut(s)	510, 1051, 1054, 1273, 1402
PleI	GAGTC	5 cut(s)	37, 161, 691, 724, 850
PpsI	GAGTC	5 cut(s)	37, 161, 691, 724, 850
PshAI	GACNNNNGTC	1 cut(s)	1488
PshBI	ATTAAT	2 cut(s)	1131, 1343
PsiI	TTATAA	1 cut(s)	893
Psp124BI	GAGCTC	2 cut(s)	1201, 1896
Psp6I	CCWGG	2 cut(s)	310, 954
PspEI	GGTNACC	1 cut(s)	1481
PspGI	CCWGG	2 cut(s)	310, 954
PspN4I	GGNNCC	3 cut(s)	150, 293, 1739
PspPI	GGNCC	2 cut(s)	240, 318
PsuI	RGATCY	1 cut(s)	1853
PvuII	CAGCTG	2 cut(s)	26, 1274
RsaI	GTAC	5 cut(s)	584, 703, 860, 1097, 1368
RsaNI	GTAC	5 cut(s)	583, 702, 859, 1096, 1367
RseI	CAYNNNNRTG	2 cut(s)	760, 1328
SacI	GAGCTC	2 cut(s)	1201, 1896
SapI	GCTCTTC	1 cut(s)	1884
SatI	GCNGC	5 cut(s)	509, 1050, 1053, 1272, 1401
Sau3AI	GATC	8 cut(s)	357, 689, 846, 910, 918, 988, 1744, 1853
Sau96I	GGNCC	2 cut(s)	240, 318
ScaI	AGTACT	1 cut(s)	1368
SchI	GAGTC	5 cut(s)	37, 161, 691, 724, 851
ScrFI	CCNGG	2 cut(s)	312, 956
SduI	GDGCHC	4 cut(s)	162, 930, 1201, 1896
SfaNI	GCATC	2 cut(s)	892, 1314
SfcI	CTRYAG	1 cut(s)	1539
Sfr274I	CTCGAG	1 cut(s)	434
SlaI	CTCGAG	1 cut(s)	434
SmiMI	CAYNNNNRTG	2 cut(s)	760, 1328
SmlI	CTYRAG	3 cut(s)	434, 443, 800
SmoI	CTYRAG	3 cut(s)	434, 443, 800
SsiI	CCGC	3 cut(s)	1486, 1508, 1727
SspI	AATATT	1 cut(s)	1884
SspMI	CTAG	2 cut(s)	414, 669
SstI	GAGCTC	2 cut(s)	1201, 1896
StyD4I	CCNGG	2 cut(s)	310, 954
TaaI	ACNGT	6 cut(s)	153, 271, 382, 424, 1543, 1645
TaiI	ACGT	3 cut(s)	650, 986, 1143
TaqI	TCGA	6 cut(s)	426, 435, 845, 991, 1156, 1659
TaqII	GACCGA	1 cut(s)	60
TatI	WGTACW	4 cut(s)	582, 858, 1095, 1366
TfiI	GAWTC	2 cut(s)	637, 1036
TscAI	CASTG	2 cut(s)	162, 935
TseFI	GTSAC	3 cut(s)	72, 1297, 1481
TseI	GCWGC	5 cut(s)	508, 1049, 1052, 1271, 1400
Tsp45I	GTSAC	3 cut(s)	72, 1297, 1481
TspDTI	ATGAA	8 cut(s)	219, 610, 614, 1114, 1149, 1366, 1541, 1832
TspGWI	ACGGA	1 cut(s)	1864
TspRI	CASTG	2 cut(s)	162, 935
Vha464I	CTTAAG	2 cut(s)	443, 800
VspI	ATTAAT	2 cut(s)	1131, 1343
XapI	RAATTY	2 cut(s)	30, 1829
XhoI	CTCGAG	1 cut(s)	434
XmiI	GTMKAC	2 cut(s)	1224, 1572
XspI	CTAG	2 cut(s)	414, 669
ZrmI	AGTACT	1 cut(s)	1368

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.