Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

23997800 .. 24000846

Gene Structure 3047 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g31230.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGGAGAAGCAACAATCTGATAATGGAGGTGAAGTTGTATTGGAAGTTGGAAAGGAAAAGAGCAACAGACCAAGAAGCTCAGAATCTTCAGCTAGCCAGACCATCACCAACAGAAACTCATTCAGTAGGTCAGTTCTCTCCATACCCAAGTCAAGATTCGGTGAACAACTGCCCATTATGCATGAAGAGACTAGTTCAGTTCAGGCAACTGCTGAAAATTCCGGCAATGCTTCATCCAATAGGGAGTCCCCGAATGAAATCTCATTGGAGAGGAGTAAATCCTCCTCTGCAAAGAAGGCACCGCCGTCTCCGGGCAGAGCAGAAGATGATGATGAGGATGAAGAGGTTTACAAGGTGCCTGATTTGAGGGTGAGCAAGAAGATCTTGATTGAGTGGTTTGTTTTCCTGTGCATTTTGGGTTGTTTAGTGGCTAGCTTGACTATGAAGAAACTACAGCATTGTATGGTTTGGGGATTGGAGATTTGGAAATGGTGTGTGCTTGTTATGGTGGTATTTAGTGGCATGTTTGTTACCAATTGGGTTATGCATTTGATGCTTTTTGTGATTGAGGGAAACTTTTTGCTTAGGAAGAAGGTGTTGTATTTTGTTCATGGATTGAAGAAGAGTATTCAGGTTCTCATGTGGGTGAGTCTGGTTCTTCTCACATGGCTACTCTTGATCAAACGCGGGTTTCAGCAATCACAAGGTTCCAGTAAGATTGTGGCTTACATCACGCGAAGTATTGTTGCAGGCGTTATTGGCGCATTTTTGTGGTTGTTGAAGACTTTGTTGGTTCTGATCTTAGCATCTAAATTTGAGGCGGTCACCTATTTTGATAGAATTCAAGAATCGATACTCTTTCAATATATTCTTCAGGCAATTTCAGGAACTCCGTTTATGGAAGAGGATTATGAACGATCACGAGGCAGTAGCAAACTGAGTTACAGAGAGACTAAGAAGGCACAGGCAGAAGGGGTCTCCAGGACAAAGATCAGGGCAGAAAGGGAGGTCAGTGACAAGTCAAAGATTCAAGGATCAAATGTTTCTTCTTGGACCTTGAATGGATTGGTGAATGTAGTAACCAGTTCAGGGCTGACCATCTCTCTAAAAGGACATAGTGGTAAAGAGATTACCAGTGAGATGGAAGCAACTGCTGCTGCAGACGACATTTTCCTTCATGTCGCTCCTCCTGGTGCTAAGTACATTGAGGAGTGGGATCTCATGAGGTTCATGACGGAGGAAGAAGTAGACCTTGTATGGCCGCTGATTGACGTAGCAATGACCGGAAAAGTTGACAGAAAAACTCTAACAGACTGGGTGCTGAAGGTCTACACTGATCGCAAAGCATTAGCTCATGCATTGTCTGATACCAAAGCAGTTACAGCGCAAGTCGACAATCTTGTGACTAGCATTGTGTTCATTATCATCATCGTCGTGAGCCTTCTAGTTGTGGAAATTGCAACCACAAGCTTTTTTGTGTTCCTATCATCACAGCTGGTATTAGCAGCTTTTGCTTTCGGAAACACTTGCAAGAATGTATTTGAATCTATCATGTTTCTATTTGTGGCACATCCTTATGATGTTGGAGACTGCATTGTTTTTGATGGCGTTCCGATGATAGTTGAAGAGATGAGCATCTTTACTACGGTTTGCTTGAAACTCAGTAGTAACGAGAAGGTGTACTATCCAAATTCCGTGTTATCTGCAACATCAATAAGCAATTACACCAGAAGTCCAGATATGAGAGAAGCTGTGGAGTTCTCAATTGCTTTAACACCAGTGGAGAATATAGTCAAGTTGAAAGAGAAAATAAAGGAATACATCGAGGGGAAGCCTCAATATTGGCATCGAAAACACAATGTGGCGGTGATTAACATTGAAGATGTTAACAAGTTAAAGATGGCCGTATATTGTCATCACAAATTGAACTTTAGAGATTTCGAGGAAAATAATTTGCGTAGAGCTGAGCTAAACTTCGAATTGCTGAAGATTCTTGAAGAGCTAAACATCACAGTGATGTCAGATGTATCCTCCCCAAAACTGAATCACACTCCAACAGATAAGATATGCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

692

Amino Acids

77.93

Weight (kDa)

6.54

Isoelectric Point (pI)

39.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	509 - 577	1.5e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	298, 355
AccI	GTMKAC	3 cut(s)	1248, 1329, 1392
AccII	CGCG	2 cut(s)	687, 736
AciI	CCGC	5 cut(s)	302, 687, 821, 1262, 1865
AclWI	GGATC	2 cut(s)	1042, 1224
AcoI	YGGCCR	2 cut(s)	1259, 1902
AcsI	RAATTY	4 cut(s)	217, 812, 840, 1690
AcuI	CTGAAG	4 cut(s)	72, 857, 1343, 2006
AdeI	CACNNNGTG	1 cut(s)	1861
AfaI	GTAC	2 cut(s)	1202, 1682
AfiI	CCNNNNNNNGG	2 cut(s)	311, 1089
AhlI	ACTAGT	1 cut(s)	191
AjnI	CCWGG	2 cut(s)	980, 1189
AjuI	GAANNNNNNNTTGG	4 cut(s)	140, 172, 773, 805
Alw26I	GTCTC	5 cut(s)	182, 312, 944, 982, 1581
AlwI	GGATC	2 cut(s)	1042, 1224
AoxI	GGCC	2 cut(s)	1259, 1902
ApeKI	GCWGC	3 cut(s)	1154, 1157, 1505
ApoI	RAATTY	4 cut(s)	217, 812, 840, 1690
AspLEI	GCGC	2 cut(s)	764, 1387
AspS9I	GGNCC	1 cut(s)	1053
AsuC2I	CCSGG	1 cut(s)	312
AsuHPI	GGTGA	8 cut(s)	41, 97, 173, 382, 658, 817, 1081, 1879
AsuII	TTCGAA	1 cut(s)	1977
AsuNHI	GCTAGC	2 cut(s)	92, 431
AvaII	GGWCC	1 cut(s)	1053
BanI	GGYRCC	2 cut(s)	298, 355
BauI	CACGAG	1 cut(s)	921
BbsI	GAAGAC	1 cut(s)	788
BbvI	GCAGC	3 cut(s)	1141, 1144, 1517
BccI	CCATC	5 cut(s)	110, 1106, 1135, 1598, 1894
BceAI	ACGGC	2 cut(s)	289, 1889
BciT130I	CCWGG	2 cut(s)	982, 1191
BciVI	GTATCC	1 cut(s)	2038
BclI	TGATCA	1 cut(s)	678
BcnI	CCSGG	1 cut(s)	312
BcoDI	GTCTC	5 cut(s)	182, 312, 944, 982, 1581
BcuI	ACTAGT	1 cut(s)	191
BfaI	CTAG	5 cut(s)	93, 192, 432, 1407, 1445
BfmI	CTRYAG	2 cut(s)	452, 1158
BfuI	GTATCC	1 cut(s)	2038
BglII	AGATCT	1 cut(s)	381
BisI	GCNGC	4 cut(s)	1155, 1158, 1262, 1506
BlpI	GCTNAGC	1 cut(s)	1965
BlsI	GCNGC	4 cut(s)	1156, 1159, 1263, 1507
Bme1390I	CCNGG	3 cut(s)	312, 982, 1191
Bme18I	GGWCC	1 cut(s)	1053
BmgT120I	GGNCC	1 cut(s)	1053
BmiI	GGNNCC	3 cut(s)	300, 357, 709
BmrFI	CCNGG	3 cut(s)	312, 982, 1191
BmrI	ACTGGG	1 cut(s)	1324
BmsI	GCATC	4 cut(s)	543, 815, 1644, 1855
BmtI	GCTAGC	2 cut(s)	96, 435
BmuI	ACTGGG	1 cut(s)	1324
BpiI	GAAGAC	1 cut(s)	788
BpmI	CTGGAG	1 cut(s)	964
Bpu10I	CCTNAGC	1 cut(s)	584
Bpu1102I	GCTNAGC	1 cut(s)	1965
Bpu14I	TTCGAA	1 cut(s)	1977
BpuMI	CCSGG	1 cut(s)	312
Bsa29I	ATCGAT	1 cut(s)	851
BsaBI	GATNNNNATC	1 cut(s)	1634
BsaI	GGTCTC	1 cut(s)	982
BsaWI	WCCGGW	1 cut(s)	1283
BsaXI	ACNNNNNCTCC	2 cut(s)	18, 48
Bsc4I	CCNNNNNNNGG	2 cut(s)	311, 1089
Bse1I	ACTGG	5 cut(s)	711, 1083, 1134, 1319, 1778
Bse3DI	GCAATG	2 cut(s)	232, 1284
Bse8I	GATNNNNATC	1 cut(s)	1634
BseBI	CCWGG	2 cut(s)	982, 1191
BseCI	ATCGAT	1 cut(s)	851
BseGI	GGATG	3 cut(s)	233, 343, 1570
BseJI	GATNNNNATC	1 cut(s)	1634
BseLI	CCNNNNNNNGG	2 cut(s)	311, 1089
BseMI	GCAATG	2 cut(s)	232, 1284
BseMII	CTCAG	4 cut(s)	93, 929, 1675, 1956
BseNI	ACTGG	5 cut(s)	711, 1083, 1134, 1319, 1778
BseRI	GAGGAG	4 cut(s)	274, 286, 1176, 1223
BseXI	GCAGC	3 cut(s)	1141, 1144, 1517
Bsh1236I	CGCG	2 cut(s)	687, 736
BshFI	GGCC	2 cut(s)	1261, 1904
BshNI	GGYRCC	2 cut(s)	298, 355
BshVI	ATCGAT	1 cut(s)	851
BsiSI	CCGG	3 cut(s)	222, 311, 1284
BslFI	GGGAC	1 cut(s)	232
BslI	CCNNNNNNNGG	2 cut(s)	311, 1089
BsmAI	GTCTC	5 cut(s)	182, 312, 944, 982, 1581
BsmBI	CGTCTC	1 cut(s)	312
BsmFI	GGGAC	1 cut(s)	232
BsnI	GGCC	2 cut(s)	1261, 1904
Bso31I	GGTCTC	1 cut(s)	982
Bsp119I	TTCGAA	1 cut(s)	1977
Bsp143I	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
Bsp1720I	GCTNAGC	1 cut(s)	1965
BspACI	CCGC	5 cut(s)	302, 687, 821, 1262, 1865
BspANI	GGCC	2 cut(s)	1261, 1904
BspCNI	CTCAG	4 cut(s)	92, 930, 1674, 1957
BspDI	ATCGAT	1 cut(s)	851
BspFNI	CGCG	2 cut(s)	687, 736
BspHI	TCATGA	2 cut(s)	1221, 1230
BspLI	GGNNCC	3 cut(s)	300, 357, 709
BspMAI	CTGCAG	1 cut(s)	1162
BspOI	GCTAGC	2 cut(s)	96, 435
BspPI	GGATC	2 cut(s)	1042, 1224
BspQI	GCTCTTC	1 cut(s)	1992
BspT104I	TTCGAA	1 cut(s)	1977
BspT107I	GGYRCC	2 cut(s)	298, 355
BspTNI	GGTCTC	1 cut(s)	982
BsrDI	GCAATG	2 cut(s)	232, 1284
BsrI	ACTGG	5 cut(s)	711, 1083, 1134, 1319, 1778
BssMI	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
BssSI	CACGAG	1 cut(s)	921
Bst2BI	CACGAG	1 cut(s)	921
Bst2UI	CCWGG	2 cut(s)	982, 1191
Bst4CI	ACNGT	2 cut(s)	1648, 2014
Bst6I	CTCTTC	6 cut(s)	180, 336, 617, 897, 1620, 1992
BstAPI	GCANNNNNTGC	3 cut(s)	553, 1154, 1511
BstBI	TTCGAA	1 cut(s)	1977
BstC8I	GCNNGC	3 cut(s)	94, 433, 751
BstDEI	CTNAG	8 cut(s)	79, 584, 802, 938, 954, 1197, 1661, 1965
BstEII	GGTNACC	1 cut(s)	823
BstF5I	GGATG	3 cut(s)	233, 343, 1570
BstFNI	CGCG	2 cut(s)	687, 736
BstHHI	GCGC	2 cut(s)	764, 1387
BstKTI	GATC	8 cut(s)	384, 681, 801, 920, 993, 1037, 1219, 1339
BstMAI	GTCTC	5 cut(s)	182, 312, 944, 982, 1581
BstMBI	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
BstMWI	GCNNNNNNNGC	7 cut(s)	178, 296, 553, 759, 1154, 1382, 1511
BstNI	CCWGG	2 cut(s)	982, 1191
BstNSI	RCATGY	1 cut(s)	526
BstPI	GGTNACC	1 cut(s)	823
BstSCI	CCNGG	3 cut(s)	310, 980, 1189
BstSFI	CTRYAG	2 cut(s)	452, 1158
BstUI	CGCG	2 cut(s)	687, 736
BstV1I	GCAGC	3 cut(s)	1141, 1144, 1517
BstV2I	GAAGAC	1 cut(s)	788
BstX2I	RGATCY	2 cut(s)	381, 1216
BstXI	CCANNNNNNTGG	1 cut(s)	1141
BstYI	RGATCY	2 cut(s)	381, 1216
Bsu15I	ATCGAT	1 cut(s)	851
BsuI	GTATCC	1 cut(s)	2038
BsuRI	GGCC	2 cut(s)	1261, 1904
BsuTUI	ATCGAT	1 cut(s)	851
BtsCI	GGATG	3 cut(s)	233, 343, 1570
BtsIMutI	CAGTG	5 cut(s)	1018, 1141, 1332, 1785, 2019
Cac8I	GCNNGC	3 cut(s)	94, 433, 751
CciI	TCATGA	2 cut(s)	1221, 1230
CfoI	GCGC	2 cut(s)	764, 1387
Cfr13I	GGNCC	1 cut(s)	1053
ClaI	ATCGAT	1 cut(s)	851
Csp6I	GTAC	2 cut(s)	1201, 1681
CviQI	GTAC	2 cut(s)	1201, 1681
DdeI	CTNAG	8 cut(s)	79, 584, 802, 938, 954, 1197, 1661, 1965
DpnI	GATC	8 cut(s)	383, 680, 800, 919, 992, 1036, 1218, 1338
DpnII	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
DraIII	CACNNNGTG	1 cut(s)	1861
EaeI	YGGCCR	2 cut(s)	1259, 1902
Eam1104I	CTCTTC	6 cut(s)	180, 336, 617, 897, 1620, 1992
EarI	CTCTTC	6 cut(s)	180, 336, 617, 897, 1620, 1992
Eco31I	GGTCTC	1 cut(s)	982
Eco47I	GGWCC	1 cut(s)	1053
Eco57I	CTGAAG	4 cut(s)	72, 857, 1343, 2006
Eco91I	GGTNACC	1 cut(s)	823
EcoO65I	GGTNACC	1 cut(s)	823
EcoRI	GAATTC	1 cut(s)	840
EcoRII	CCWGG	2 cut(s)	980, 1189
EcoT22I	ATGCAT	4 cut(s)	183, 549, 1360, 2072
Esp3I	CGTCTC	1 cut(s)	312
FalI	AAGNNNNNCTT	4 cut(s)	368, 400, 1236, 1268
FaqI	GGGAC	1 cut(s)	232
FauI	CCCGC	1 cut(s)	680
FbaI	TGATCA	1 cut(s)	678
FblI	GTMKAC	3 cut(s)	1248, 1329, 1392
Fnu4HI	GCNGC	4 cut(s)	1155, 1158, 1262, 1506
FokI	GGATG	3 cut(s)	220, 350, 1557
Fsp4HI	GCNGC	4 cut(s)	1155, 1158, 1262, 1506
FspBI	CTAG	5 cut(s)	93, 192, 432, 1407, 1445
GlaI	GCGC	2 cut(s)	763, 1386
GluI	GCNGC	4 cut(s)	1155, 1158, 1262, 1506
GsuI	CTGGAG	1 cut(s)	964
HaeIII	GGCC	2 cut(s)	1261, 1904
HapII	CCGG	3 cut(s)	222, 311, 1284
HhaI	GCGC	2 cut(s)	764, 1387
Hin6I	GCGC	2 cut(s)	762, 1385
HinP1I	GCGC	2 cut(s)	762, 1385
HincII	GTYRAC	3 cut(s)	1294, 1393, 1888
HindII	GTYRAC	3 cut(s)	1294, 1393, 1888
HindIII	AAGCTT	1 cut(s)	1468
HinfI	GANTC	9 cut(s)	83, 156, 245, 649, 848, 1027, 1544, 1990, 2044
HpaI	GTTAAC	1 cut(s)	1888
HpaII	CCGG	3 cut(s)	222, 311, 1284
HphI	GGTGA	8 cut(s)	41, 97, 173, 382, 658, 817, 1081, 1879
Hpy166II	GTNNAC	8 cut(s)	164, 349, 1249, 1294, 1330, 1393, 1681, 1888
Hpy188I	TCNGA	7 cut(s)	19, 82, 798, 1366, 1520, 1614, 2023
Hpy8I	GTNNAC	8 cut(s)	164, 349, 1249, 1294, 1330, 1393, 1681, 1888
Hpy99I	CGWCG	1 cut(s)	1436
HpyAV	CCTTC	8 cut(s)	289, 586, 952, 965, 1184, 1318, 1451, 1669
HpyCH4III	ACNGT	2 cut(s)	1648, 2014
HpyCH4IV	ACGT	1 cut(s)	1272
HpyF10VI	GCNNNNNNNGC	7 cut(s)	178, 296, 553, 759, 1154, 1382, 1511
HpyF3I	CTNAG	8 cut(s)	79, 584, 802, 938, 954, 1197, 1661, 1965
HpySE526I	ACGT	1 cut(s)	1272
HspAI	GCGC	2 cut(s)	762, 1385
Ksp22I	TGATCA	1 cut(s)	678
KspAI	GTTAAC	1 cut(s)	1888
Kzo9I	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
LguI	GCTCTTC	1 cut(s)	1992
LmnI	GCTCC	1 cut(s)	1189
Lsp1109I	GCAGC	3 cut(s)	1141, 1144, 1517
LweI	GCATC	4 cut(s)	543, 815, 1644, 1855
MaeI	CTAG	5 cut(s)	93, 192, 432, 1407, 1445
MaeII	ACGT	1 cut(s)	1272
MaeIII	GTNAC	8 cut(s)	529, 823, 941, 1013, 1078, 1378, 1402, 1667
MalI	GATC	8 cut(s)	383, 680, 800, 919, 992, 1036, 1218, 1338
MboI	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
MfeI	CAATTG	2 cut(s)	535, 1764
MflI	RGATCY	2 cut(s)	381, 1216
MlyI	GAGTC	2 cut(s)	254, 658
MmeI	TCCRAC	2 cut(s)	28, 1564
Mph1103I	ATGCAT	4 cut(s)	183, 549, 1360, 2072
MseI	TTAA	5 cut(s)	1772, 1872, 1887, 1895, 2074
MslI	CAYNNNNRTG	6 cut(s)	644, 769, 1433, 1575, 2012, 2015
MspA1I	CMGCKG	2 cut(s)	1264, 1495
MspI	CCGG	3 cut(s)	222, 311, 1284
MspR9I	CCNGG	3 cut(s)	312, 982, 1191
MunI	CAATTG	2 cut(s)	535, 1764
MvaI	CCWGG	2 cut(s)	982, 1191
MvnI	CGCG	2 cut(s)	687, 736
MwoI	GCNNNNNNNGC	7 cut(s)	178, 296, 553, 759, 1154, 1382, 1511
NciI	CCSGG	1 cut(s)	312
NdeII	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
NheI	GCTAGC	2 cut(s)	92, 431
NlaIV	GGNNCC	3 cut(s)	300, 357, 709
NmuCI	GTSAC	3 cut(s)	823, 1013, 1402
NsiI	ATGCAT	4 cut(s)	183, 549, 1360, 2072
NspI	RCATGY	1 cut(s)	526
NspV	TTCGAA	1 cut(s)	1977
PagI	TCATGA	2 cut(s)	1221, 1230
PciSI	GCTCTTC	1 cut(s)	1992
PfeI	GAWTC	7 cut(s)	83, 156, 848, 1027, 1544, 1990, 2044
PfoI	TCCNGGA	1 cut(s)	980
PkrI	GCNGC	4 cut(s)	1156, 1159, 1263, 1507
PleI	GAGTC	2 cut(s)	253, 657
PpsI	GAGTC	2 cut(s)	253, 657
Psp6I	CCWGG	2 cut(s)	980, 1189
PspEI	GGTNACC	1 cut(s)	823
PspGI	CCWGG	2 cut(s)	980, 1189
PspN4I	GGNNCC	3 cut(s)	300, 357, 709
PspPI	GGNCC	1 cut(s)	1053
PstI	CTGCAG	1 cut(s)	1162
PsuI	RGATCY	2 cut(s)	381, 1216
PvuII	CAGCTG	1 cut(s)	1495
RsaI	GTAC	2 cut(s)	1202, 1682
RsaNI	GTAC	2 cut(s)	1201, 1681
RseI	CAYNNNNRTG	6 cut(s)	644, 769, 1433, 1575, 2012, 2015
SalI	GTCGAC	1 cut(s)	1391
SapI	GCTCTTC	1 cut(s)	1992
SaqAI	TTAA	5 cut(s)	1772, 1872, 1887, 1895, 2074
SatI	GCNGC	4 cut(s)	1155, 1158, 1262, 1506
Sau3AI	GATC	8 cut(s)	381, 678, 798, 917, 990, 1034, 1216, 1336
Sau96I	GGNCC	1 cut(s)	1053
SchI	GAGTC	2 cut(s)	254, 658
ScrFI	CCNGG	3 cut(s)	312, 982, 1191
SfaNI	GCATC	4 cut(s)	543, 815, 1644, 1855
SfcI	CTRYAG	2 cut(s)	452, 1158
SfuI	TTCGAA	1 cut(s)	1977
SinI	GGWCC	1 cut(s)	1053
SmiMI	CAYNNNNRTG	6 cut(s)	644, 769, 1433, 1575, 2012, 2015
SpeI	ACTAGT	1 cut(s)	191
SsiI	CCGC	5 cut(s)	302, 687, 821, 1262, 1865
SspI	AATATT	1 cut(s)	1841
SspMI	CTAG	5 cut(s)	93, 192, 432, 1407, 1445
StyD4I	CCNGG	3 cut(s)	310, 980, 1189
TaaI	ACNGT	2 cut(s)	1648, 2014
TaiI	ACGT	1 cut(s)	1275
TaqI	TCGA	6 cut(s)	851, 1392, 1824, 1849, 1941, 1977
TatI	WGTACW	2 cut(s)	1200, 1680
TauI	GCSGC	1 cut(s)	1264
TfiI	GAWTC	7 cut(s)	83, 156, 848, 1027, 1544, 1990, 2044
Tru1I	TTAA	5 cut(s)	1772, 1872, 1887, 1895, 2074
Tru9I	TTAA	5 cut(s)	1772, 1872, 1887, 1895, 2074
TscAI	CASTG	5 cut(s)	1018, 1141, 1339, 1785, 2019
TseFI	GTSAC	3 cut(s)	823, 1013, 1402
TseI	GCWGC	3 cut(s)	1154, 1157, 1505
Tsp45I	GTSAC	3 cut(s)	823, 1013, 1402
TspGWI	ACGGA	3 cut(s)	882, 1250, 1684
TspRI	CASTG	5 cut(s)	1018, 1141, 1339, 1785, 2019
VpaK11BI	GGWCC	1 cut(s)	1053
XapI	RAATTY	4 cut(s)	217, 812, 840, 1690
XceI	RCATGY	1 cut(s)	526
XcmI	CCANNNNNNNNNTGG	1 cut(s)	718
XmiI	GTMKAC	3 cut(s)	1248, 1329, 1392
XspI	CTAG	5 cut(s)	93, 192, 432, 1407, 1445
Zsp2I	ATGCAT	4 cut(s)	183, 549, 1360, 2072

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.