Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Forward (+)

36431466 .. 36433935

Gene Structure 2470 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G018310.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1965 bp

ATGTATGAAGAGACTAGTTCAGATCAGGCAGCTGGGGCAAATTCCCCCAATGCTTCATTCAATAGGGAGTCCCCAAATGAAATCTCATTGGATAGGACTGAATCCTCCTCTGCGAAGAATGCACCGCCTTCTCCGGGCAGAACACAAGATGAGGAAGCTGAAGAGGTTTACAAGACGGCTAATGCGAAGAATGCACCACCAACTCCAGTTATAGCAGAAGATGAGGAGGATGAAGAGATATACAACAAGGTTAACTTGAGTATAGATAAGCGTGGGAAAGTGAATAAGAAGCTCTCGGGGGAGTTGATTGTTTTTCTGTCCATTTTGGGTTGTTTAGTGGCTAGCTTGACTATCCATAAGCTAAAGGATCTTATGGTCTGGGACTTGGAGATTTGGAAATGGTGTGTGCTTGTAATGGTGGTATCATGTGGCATGTTGATGACAAATTGGGTAATGCATTTCATAGTTTTCCTGATTGAGCGCAGCTTTTTGTTTAGGAAGAATGTGTTGTATTTTGTTTACGGATTGAAGAAGAATGCTCAGATTCTCATGTGGGTGGGTCTTGTTCTTCTCACATGGCTACTCTTGATCAAACGCGGGCTTCAGCAATCAAAAGTTTCCACTAAGATTCTGGCTTACATCACGCGAAGTATTGTTGCAGTTGTTATTGGGGCATCTTTGTGGTTGTTAAAGACTTTGTTGGTTAAGATCTTAGCATCTAAATTCCAGGTGGTAACCTATTTTAGTAGAATTCAAGAATCGATATTCCATCAGTATGCTCTTCGGACACTTTCAGGACCTCCGCTTATGGAGGATGCAGAAGGCAGTATCACACTGAGTTTTACTAACAAGGCACATGCAGCAGGGAAGGAGATAGAGGTCACTGACAGGGCAAAGCTTCAAAAGATAAAGCAAGAGAAGGTTTCTTCTTTTACCATGAAGGGACTGGTGGATGTAGTAACTACTTTCGGGCTGTCCACGATGTCCCAGACATTGGACGAGAAGGAAGATCTGAATGAACAGAGAGATAAGGAGATTACCAGTGAGACGGAAGCAACTGCTGCTGGCAATGACATATTCCTTAATGTAGCTCCACATGGTGCCAAGTACATTGAGGAGCGGTATCTATTGAGGTTCATGATGAAGGAAGAGGTAGACCTTGTATGGCCACTGATTGACGTAGCAAAGACTGGACAAGTTGACAGAAAAACTCTAACAGAGTGGGTGGTGAAGGTCTACACTGCTCGCAAAGCATTAGCTCATGCATTGTCTGATACCAAAGCAGTTACAGCGCAAGTCGACAATCTTGTGAGTAGCATTGTGTTGATTATCATCATCGTCGTGGGTCTTCTAGTGGTGGAAATTGCAACCACAAGCTTTTTTGTCTTCCTATCATCACAGCTCGTATTAGCGGCTTTTGCTTTTGGAAACACTTGCAAGAATGTATTTGAATCTATCATGTTTCTATTTGTGGCGCATCCATATGATGTTGGTGACTGCATTGTTTTTGAAGGCGTACCGATGATAGTTGAAGAGATGAACATCTTTACTACGGTTTTCTTGAAACTCAGTAATAACGAGAAGGTGTACTATCCAAATTCAGTTTTATCTGCAACATCGATCAGCAATTACTCCAGAAGTCCAGATATGAGAGATGTTGTAGAGTTCTCAATTGCATTAACACCTGTGGAGAAGATAGTCAATTTGAAAGAGAAAATAAAGGAATACATCGAGGGGAAGCCTCAATATTGGCATCGAAAACACAATGTGGCGGTGATTGACATAGAAGATGTTGATAAGTTAAAGATGGCCGTCTATTGTCAACACAAAATGAACTTTCAAGATTTTGAGGAAAAGAATTTGCGTAGAGCTGAGCTAAACTTCGAATTGCTGAAAATTCTTGAAGAGCTAAACATCACAGTAATATCAGCTGTCCTCCCAAAAAGTGAATCTCACCCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

654

Amino Acids

73.86

Weight (kDa)

5.83

Isoelectric Point (pI)

38.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	478 - 546	6.4e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1100
AccB7I	CCANNNNNTGG	1 cut(s)	994
AccBSI	CCGCTC	1 cut(s)	1120
AccI	GTMKAC	3 cut(s)	1155, 1236, 1299
AccII	CGCG	2 cut(s)	597, 646
AciI	CCGC	6 cut(s)	125, 597, 803, 1120, 1412, 1772
AclWI	GGATC	1 cut(s)	375
AcoI	YGGCCR	2 cut(s)	1166, 1809
AcsI	RAATTY	6 cut(s)	40, 722, 750, 1597, 1858, 1896
AcuI	CTGAAG	2 cut(s)	180, 587
AdeI	CACNNNGTG	2 cut(s)	1100, 1768
AfaI	GTAC	3 cut(s)	1109, 1518, 1589
AfiI	CCNNNNNNNGG	2 cut(s)	134, 994
AhlI	ACTAGT	1 cut(s)	14
AjnI	CCWGG	1 cut(s)	726
AjuI	GAANNNNNNNTTGG	2 cut(s)	41, 73
Alw26I	GTCTC	2 cut(s)	5, 1040
AlwI	GGATC	1 cut(s)	375
Ama87I	CYCGRG	1 cut(s)	295
AoxI	GGCC	2 cut(s)	1166, 1809
ApeKI	GCWGC	4 cut(s)	29, 483, 860, 1061
ApoI	RAATTY	6 cut(s)	40, 722, 750, 1597, 1858, 1896
AspLEI	GCGC	3 cut(s)	483, 1294, 1477
AspS9I	GGNCC	1 cut(s)	797
AsuC2I	CCSGG	1 cut(s)	135
AsuHPI	GGTGA	4 cut(s)	1240, 1505, 1786, 1946
AsuII	TTCGAA	1 cut(s)	1884
AsuNHI	GCTAGC	1 cut(s)	341
AvaI	CYCGRG	1 cut(s)	295
AvaII	GGWCC	1 cut(s)	797
BalI	TGGCCA	1 cut(s)	1168
BanI	GGYRCC	1 cut(s)	1100
BbsI	GAAGAC	2 cut(s)	1340, 1378
BbvI	GCAGC	4 cut(s)	41, 495, 872, 1048
BccI	CCATC	2 cut(s)	777, 1801
BceAI	ACGGC	2 cut(s)	192, 1796
BciT130I	CCWGG	1 cut(s)	728
BclI	TGATCA	1 cut(s)	588
BcnI	CCSGG	1 cut(s)	135
BcoDI	GTCTC	2 cut(s)	5, 1040
BcuI	ACTAGT	1 cut(s)	14
BfaI	CTAG	3 cut(s)	15, 342, 1352
BglII	AGATCT	2 cut(s)	708, 1009
BisI	GCNGC	5 cut(s)	30, 484, 861, 1062, 1413
BlpI	GCTNAGC	1 cut(s)	1872
BlsI	GCNGC	5 cut(s)	31, 485, 862, 1063, 1414
Bme1390I	CCNGG	2 cut(s)	135, 728
Bme18I	GGWCC	1 cut(s)	797
BmeT110I	CYCGRG	1 cut(s)	295
BmgT120I	GGNCC	1 cut(s)	797
BmiI	GGNNCC	1 cut(s)	1102
BmrFI	CCNGG	2 cut(s)	135, 728
BmsI	GCATC	5 cut(s)	683, 725, 805, 1486, 1762
BmtI	GCTAGC	1 cut(s)	345
BpiI	GAAGAC	2 cut(s)	1340, 1378
BpmI	CTGGAG	2 cut(s)	189, 1618
Bpu1102I	GCTNAGC	1 cut(s)	1872
Bpu14I	TTCGAA	1 cut(s)	1884
BpuEI	CTTGAG	1 cut(s)	277
BpuMI	CCSGG	1 cut(s)	135
Bsa29I	ATCGAT	2 cut(s)	761, 1619
BsaBI	GATNNNNATC	2 cut(s)	1331, 1541
Bsc4I	CCNNNNNNNGG	2 cut(s)	134, 994
Bse1I	ACTGG	4 cut(s)	206, 951, 1041, 1195
Bse3DI	GCAATG	1 cut(s)	1075
Bse8I	GATNNNNATC	2 cut(s)	1331, 1541
BseBI	CCWGG	1 cut(s)	728
BseCI	ATCGAT	2 cut(s)	761, 1619
BseGI	GGATG	4 cut(s)	235, 820, 958, 1477
BseJI	GATNNNNATC	2 cut(s)	1331, 1541
BseLI	CCNNNNNNNGG	2 cut(s)	134, 994
BseMI	GCAATG	1 cut(s)	1075
BseMII	CTCAG	4 cut(s)	554, 827, 1582, 1863
BseNI	ACTGG	4 cut(s)	206, 951, 1041, 1195
BseRI	GAGGAG	3 cut(s)	97, 239, 1130
BseXI	GCAGC	4 cut(s)	41, 495, 872, 1048
BseYI	CCCAGC	1 cut(s)	32
Bsh1236I	CGCG	2 cut(s)	597, 646
BshFI	GGCC	2 cut(s)	1168, 1811
BshNI	GGYRCC	1 cut(s)	1100
BshVI	ATCGAT	2 cut(s)	761, 1619
BsiHKCI	CYCGRG	1 cut(s)	295
BsiSI	CCGG	1 cut(s)	134
BslFI	GGGAC	4 cut(s)	55, 395, 957, 970
BslI	CCNNNNNNNGG	2 cut(s)	134, 994
BsmAI	GTCTC	2 cut(s)	5, 1040
BsmBI	CGTCTC	1 cut(s)	1040
BsmFI	GGGAC	4 cut(s)	55, 395, 957, 970
BsmI	GAATGC	3 cut(s)	124, 196, 541
BsnI	GGCC	2 cut(s)	1168, 1811
BsoBI	CYCGRG	1 cut(s)	295
Bsp119I	TTCGAA	1 cut(s)	1884
Bsp143I	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
Bsp1720I	GCTNAGC	1 cut(s)	1872
BspACI	CCGC	6 cut(s)	125, 597, 803, 1120, 1412, 1772
BspANI	GGCC	2 cut(s)	1168, 1811
BspCNI	CTCAG	4 cut(s)	553, 828, 1581, 1864
BspDI	ATCGAT	2 cut(s)	761, 1619
BspFNI	CGCG	2 cut(s)	597, 646
BspHI	TCATGA	1 cut(s)	1137
BspLI	GGNNCC	1 cut(s)	1102
BspOI	GCTAGC	1 cut(s)	345
BspPI	GGATC	1 cut(s)	375
BspQI	GCTCTTC	2 cut(s)	786, 1899
BspT104I	TTCGAA	1 cut(s)	1884
BspT107I	GGYRCC	1 cut(s)	1100
BsrBI	CCGCTC	1 cut(s)	1120
BsrDI	GCAATG	1 cut(s)	1075
BsrI	ACTGG	4 cut(s)	206, 951, 1041, 1195
BssMI	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
Bst2UI	CCWGG	1 cut(s)	728
Bst4CI	ACNGT	2 cut(s)	1555, 1921
Bst6I	CTCTTC	7 cut(s)	3, 156, 228, 786, 1143, 1527, 1899
BstAPI	GCANNNNNTGC	1 cut(s)	1061
BstBI	TTCGAA	1 cut(s)	1884
BstC8I	GCNNGC	4 cut(s)	343, 599, 1066, 1246
BstDEI	CTNAG	6 cut(s)	540, 624, 712, 836, 1568, 1872
BstEII	GGTNACC	1 cut(s)	733
BstF5I	GGATG	4 cut(s)	235, 820, 958, 1477
BstFNI	CGCG	2 cut(s)	597, 646
BstHHI	GCGC	3 cut(s)	483, 1294, 1477
BstKTI	GATC	6 cut(s)	25, 370, 591, 711, 1012, 1623
BstMAI	GTCTC	2 cut(s)	5, 1040
BstMBI	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
BstMWI	GCNNNNNNNGC	8 cut(s)	35, 119, 191, 860, 1061, 1250, 1289, 1418
BstNI	CCWGG	1 cut(s)	728
BstNSI	RCATGY	2 cut(s)	436, 860
BstPI	GGTNACC	1 cut(s)	733
BstSCI	CCNGG	2 cut(s)	133, 726
BstUI	CGCG	2 cut(s)	597, 646
BstV1I	GCAGC	4 cut(s)	41, 495, 872, 1048
BstV2I	GAAGAC	2 cut(s)	1340, 1378
BstX2I	RGATCY	3 cut(s)	367, 708, 1009
BstYI	RGATCY	3 cut(s)	367, 708, 1009
Bsu15I	ATCGAT	2 cut(s)	761, 1619
BsuRI	GGCC	2 cut(s)	1168, 1811
BsuTUI	ATCGAT	2 cut(s)	761, 1619
BtsCI	GGATG	4 cut(s)	235, 820, 958, 1477
BtsI	GCAGTG	1 cut(s)	1239
BtsIMutI	CAGTG	5 cut(s)	833, 882, 1048, 1169, 1239
Cac8I	GCNNGC	4 cut(s)	343, 599, 1066, 1246
CciI	TCATGA	1 cut(s)	1137
CfoI	GCGC	3 cut(s)	483, 1294, 1477
Cfr13I	GGNCC	1 cut(s)	797
ClaI	ATCGAT	2 cut(s)	761, 1619
Csp6I	GTAC	3 cut(s)	1108, 1517, 1588
CviQI	GTAC	3 cut(s)	1108, 1517, 1588
DdeI	CTNAG	6 cut(s)	540, 624, 712, 836, 1568, 1872
DpnI	GATC	6 cut(s)	24, 369, 590, 710, 1011, 1622
DpnII	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
DraIII	CACNNNGTG	2 cut(s)	1100, 1768
EaeI	YGGCCR	2 cut(s)	1166, 1809
Eam1104I	CTCTTC	7 cut(s)	3, 156, 228, 786, 1143, 1527, 1899
EarI	CTCTTC	7 cut(s)	3, 156, 228, 786, 1143, 1527, 1899
Eco47I	GGWCC	1 cut(s)	797
Eco57I	CTGAAG	2 cut(s)	180, 587
Eco88I	CYCGRG	1 cut(s)	295
Eco91I	GGTNACC	1 cut(s)	733
EcoO109I	RGGNCCY	1 cut(s)	797
EcoO65I	GGTNACC	1 cut(s)	733
EcoRI	GAATTC	1 cut(s)	750
EcoRII	CCWGG	1 cut(s)	726
EcoT22I	ATGCAT	2 cut(s)	459, 1267
Esp3I	CGTCTC	1 cut(s)	1040
FalI	AAGNNNNNCTT	2 cut(s)	239, 271
FaqI	GGGAC	4 cut(s)	55, 395, 957, 970
FauI	CCCGC	1 cut(s)	590
FauNDI	CATATG	1 cut(s)	1483
FbaI	TGATCA	1 cut(s)	588
FblI	GTMKAC	3 cut(s)	1155, 1236, 1299
Fnu4HI	GCNGC	5 cut(s)	30, 484, 861, 1062, 1413
FokI	GGATG	4 cut(s)	242, 827, 965, 1464
Fsp4HI	GCNGC	5 cut(s)	30, 484, 861, 1062, 1413
FspBI	CTAG	3 cut(s)	15, 342, 1352
GlaI	GCGC	3 cut(s)	482, 1293, 1476
GluI	GCNGC	5 cut(s)	30, 484, 861, 1062, 1413
GsaI	CCCAGC	1 cut(s)	36
GsuI	CTGGAG	2 cut(s)	189, 1618
HaeIII	GGCC	2 cut(s)	1168, 1811
HapII	CCGG	1 cut(s)	134
HhaI	GCGC	3 cut(s)	483, 1294, 1477
Hin6I	GCGC	3 cut(s)	481, 1292, 1475
HinP1I	GCGC	3 cut(s)	481, 1292, 1475
HincII	GTYRAC	4 cut(s)	253, 1201, 1300, 1823
HindII	GTYRAC	4 cut(s)	253, 1201, 1300, 1823
HindIII	AAGCTT	2 cut(s)	896, 1375
HinfI	GANTC	7 cut(s)	68, 101, 544, 628, 758, 1451, 1949
HpaI	GTTAAC	1 cut(s)	253
HpaII	CCGG	1 cut(s)	134
HphI	GGTGA	4 cut(s)	1240, 1505, 1786, 1946
Hpy188I	TCNGA	5 cut(s)	22, 543, 786, 1014, 1273
Hpy99I	CGWCG	1 cut(s)	1343
HpyCH4III	ACNGT	2 cut(s)	1555, 1921
HpyCH4IV	ACGT	1 cut(s)	1179
HpyF10VI	GCNNNNNNNGC	8 cut(s)	35, 119, 191, 860, 1061, 1250, 1289, 1418
HpyF3I	CTNAG	6 cut(s)	540, 624, 712, 836, 1568, 1872
HpySE526I	ACGT	1 cut(s)	1179
HspAI	GCGC	3 cut(s)	481, 1292, 1475
Ksp22I	TGATCA	1 cut(s)	588
KspAI	GTTAAC	1 cut(s)	253
Kzo9I	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
LguI	GCTCTTC	2 cut(s)	786, 1899
LmnI	GCTCC	2 cut(s)	1096, 1117
Lsp1109I	GCAGC	4 cut(s)	41, 495, 872, 1048
LweI	GCATC	5 cut(s)	683, 725, 805, 1486, 1762
MaeI	CTAG	3 cut(s)	15, 342, 1352
MaeII	ACGT	1 cut(s)	1179
MaeIII	GTNAC	5 cut(s)	733, 880, 958, 1285, 1493
MalI	GATC	6 cut(s)	24, 369, 590, 710, 1011, 1622
MbiI	CCGCTC	1 cut(s)	1120
MboI	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
MfeI	CAATTG	1 cut(s)	1671
MflI	RGATCY	3 cut(s)	367, 708, 1009
MlsI	TGGCCA	1 cut(s)	1168
MluNI	TGGCCA	1 cut(s)	1168
MlyI	GAGTC	1 cut(s)	77
Mox20I	TGGCCA	1 cut(s)	1168
Mph1103I	ATGCAT	2 cut(s)	459, 1267
MscI	TGGCCA	1 cut(s)	1168
MseI	TTAA	6 cut(s)	252, 689, 705, 1083, 1679, 1802
MslI	CAYNNNNRTG	6 cut(s)	437, 554, 679, 774, 1340, 1482
Msp20I	TGGCCA	1 cut(s)	1168
MspA1I	CMGCKG	2 cut(s)	32, 1931
MspI	CCGG	1 cut(s)	134
MspR9I	CCNGG	2 cut(s)	135, 728
MunI	CAATTG	1 cut(s)	1671
Mva1269I	GAATGC	3 cut(s)	124, 196, 541
MvaI	CCWGG	1 cut(s)	728
MvnI	CGCG	2 cut(s)	597, 646
MwoI	GCNNNNNNNGC	8 cut(s)	35, 119, 191, 860, 1061, 1250, 1289, 1418
NciI	CCSGG	1 cut(s)	135
NdeI	CATATG	1 cut(s)	1483
NdeII	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
NheI	GCTAGC	1 cut(s)	341
NlaIV	GGNNCC	1 cut(s)	1102
NmuCI	GTSAC	2 cut(s)	880, 1493
NsiI	ATGCAT	2 cut(s)	459, 1267
NspI	RCATGY	2 cut(s)	436, 860
NspV	TTCGAA	1 cut(s)	1884
PagI	TCATGA	1 cut(s)	1137
PciSI	GCTCTTC	2 cut(s)	786, 1899
PcsI	WCGNNNNNNNCGW	1 cut(s)	182
PctI	GAATGC	3 cut(s)	124, 196, 541
PfeI	GAWTC	6 cut(s)	101, 544, 628, 758, 1451, 1949
PflMI	CCANNNNNTGG	1 cut(s)	994
PkrI	GCNGC	5 cut(s)	31, 485, 862, 1063, 1414
PleI	GAGTC	1 cut(s)	76
PpsI	GAGTC	1 cut(s)	76
PpuMI	RGGWCCY	1 cut(s)	797
Psp5II	RGGWCCY	1 cut(s)	797
Psp6I	CCWGG	1 cut(s)	726
PspEI	GGTNACC	1 cut(s)	733
PspFI	CCCAGC	1 cut(s)	32
PspGI	CCWGG	1 cut(s)	726
PspN4I	GGNNCC	1 cut(s)	1102
PspPI	GGNCC	1 cut(s)	797
PspPPI	RGGWCCY	1 cut(s)	797
PsuI	RGATCY	3 cut(s)	367, 708, 1009
PvuII	CAGCTG	2 cut(s)	32, 1931
RsaI	GTAC	3 cut(s)	1109, 1518, 1589
RsaNI	GTAC	3 cut(s)	1108, 1517, 1588
RseI	CAYNNNNRTG	6 cut(s)	437, 554, 679, 774, 1340, 1482
SalI	GTCGAC	1 cut(s)	1298
SapI	GCTCTTC	2 cut(s)	786, 1899
SaqAI	TTAA	6 cut(s)	252, 689, 705, 1083, 1679, 1802
SatI	GCNGC	5 cut(s)	30, 484, 861, 1062, 1413
Sau3AI	GATC	6 cut(s)	22, 367, 588, 708, 1009, 1620
Sau96I	GGNCC	1 cut(s)	797
SchI	GAGTC	1 cut(s)	77
ScrFI	CCNGG	2 cut(s)	135, 728
SfaNI	GCATC	5 cut(s)	683, 725, 805, 1486, 1762
SfuI	TTCGAA	1 cut(s)	1884
SinI	GGWCC	1 cut(s)	797
SmiMI	CAYNNNNRTG	6 cut(s)	437, 554, 679, 774, 1340, 1482
SmlI	CTYRAG	1 cut(s)	256
SmoI	CTYRAG	1 cut(s)	256
SpeI	ACTAGT	1 cut(s)	14
SsiI	CCGC	6 cut(s)	125, 597, 803, 1120, 1412, 1772
SspI	AATATT	1 cut(s)	1748
SspMI	CTAG	3 cut(s)	15, 342, 1352
StyD4I	CCNGG	2 cut(s)	133, 726
TaaI	ACNGT	2 cut(s)	1555, 1921
TaiI	ACGT	1 cut(s)	1182
TaqI	TCGA	6 cut(s)	761, 1299, 1619, 1731, 1756, 1884
TatI	WGTACW	2 cut(s)	1107, 1587
TauI	GCSGC	1 cut(s)	1415
TfiI	GAWTC	6 cut(s)	101, 544, 628, 758, 1451, 1949
Tru1I	TTAA	6 cut(s)	252, 689, 705, 1083, 1679, 1802
Tru9I	TTAA	6 cut(s)	252, 689, 705, 1083, 1679, 1802
TscAI	CASTG	5 cut(s)	840, 889, 1048, 1176, 1246
TseFI	GTSAC	2 cut(s)	880, 1493
TseI	GCWGC	4 cut(s)	29, 483, 860, 1061
Tsp45I	GTSAC	2 cut(s)	880, 1493
TspGWI	ACGGA	2 cut(s)	537, 1064
TspRI	CASTG	5 cut(s)	840, 889, 1048, 1176, 1246
Van91I	CCANNNNNTGG	1 cut(s)	994
VpaK11BI	GGWCC	1 cut(s)	797
XapI	RAATTY	6 cut(s)	40, 722, 750, 1597, 1858, 1896
XceI	RCATGY	2 cut(s)	436, 860
XcmI	CCANNNNNNNNNTGG	2 cut(s)	628, 943
XmiI	GTMKAC	3 cut(s)	1155, 1236, 1299
XspI	CTAG	3 cut(s)	15, 342, 1352
Zsp2I	ATGCAT	2 cut(s)	459, 1267

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.