Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

23992336 .. 23996220

Gene Structure 3885 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g31220.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGAAAGCCGCAGAGAAAGGTTCAAAGGGCGGCGAGATAAGCATGTCGGAGAAGCAATCTGCAAATGGAGGTGAAGTTATAGTGGAAGTCCAAAATGCTGGGACTTATGAAACTAGAGGGTCTCCTTCAAACAAGCAAAGCAAAGTGGACTTAAGACTCCCCACTGAGTCCAGTAGTAAGCCAGTTTCGAGTGCGTTTCCTTCACCGGATGTTTTAAGAGCTAGTCCCAACCCCGGTAAGCCTCCTAGGATCCCCGCCCGCAATGAGAGCATCAATAGGAGAAAGTCATTTAATAGGTCAGTTAGTAAACCCAAGTCAAGGTTTGGTGAACCATCGCCTGTTGTGAATGAAGATAGTTCAGATCAGGTTGGTTCGCCTTATAGGGGGGCTTCATTCAGTAGGGCTTCCCCAAATAACATATCAGGAGCTAGGGCCATATCCATTACTTCTAATAGGACTGTACCGCCGTCCCCAGGCAGGACCAAAGATAAGGAGGATGAAGAGATTTACAAGAAGGTTAAGTTGAGTAGAGATAAGCATGGGAAAATGAAAAAGACAGTCTTGACTGAGTTGGTTGTTTTCCTGTGCATTTTGGGTTGTTTAGTAGCTAGCTTGACTGTGAAGAGACTAGAGCATTATATGGTTTGGGGATTGGAGATTTGGAAATGGTGTGTGCTTGTCATGGTGATATTCAGTGGCATGTTGGTTACGAATTGGGTTATGCATTTGATAGTTTTTTTGATTGAGTGCAACTTTTTGCTTCGGAAGAAGGTGTTGTATTTTGTTCATGGAATGAAGAAGAGTGTTCAGGTTTTCTTGTGGCTGTCTCTGGTTCTTCTCACTTGGCTGCTGTTGTTCAACCGTGGGGTTGAGAGGTCAGAGACTTCCACCAAGATTTTAAAATATGTTACATGGACTATTGTTTCGGTTCTCATTGGGGCGTTCTTATGGATGTTAAAAACATTGTTGCTTAAGATCTTAGCATCTAGTTTCCATGTGAACACCTTCTTTGATAGAATTCAAGAATCAATCTTCCATCAGTATGTTCTTCAGACACTTTCAGGGCATCCGCTTATGGAGGAGGCTGATGGGAATGGTGGCAAATCACCGAGCACTAGCAAATTGAGCTTCAGAGCAACCAAGAAGGCAAAAGAAGGGAAGGAAAAACAGGTCATTGACATGACAAAGCTTCAAAAGATAAAGCAAGAGAAGGTTTCTTCTTGGACCATGAAGGTTTTGGTGGATGCAGTAACTAGTTCAGGGTTGTCCACGATCTCCCATACATTGGATGAGATGGAAGGTCTGAATGAACAGAGAGATAAGGAAATTACCAGTGAGATGGAAGCAACTGCTGCTGCCTATGACATATTCCTTAACGTCGCTCCGCTCGGTGCCAAGTACATTGAGGAGTGGGATCTATTGAGGTTCATGATCGAGGAAGAGGTGGAGCTTGTGTGGCCACTAATTGATGTGGCAAAGACCGGACAAGTTGATAGAAAAACTCTAACAGAGTGGGTGTTGAAGGTCTACAATGGTCGCAAAGCATTAGCTCATTCATTAACGGACACCAAAACAGCCGTGAAGCAATTGGACAAACTTGTGACTACTGTCCTGGTTATTATTACCATTGTAGTATGGCTTCTGTTGATGGAAATTGCAACTACAAAAGTACTTGTCTTACTCTCATCACAGCTTGTAGTGGCAGCTTTTATATTTGGAAACACTTGCAAGACTATATTTGAAGCTATTATCTTCGTATTTGTGATGCATCCATTTGATGTTGGGGACCGCTGTGTTGTCGATGGCGTTCAGTTAATGGTTGAGGAGATGAACATCTTAACCACAGTCTTCCTGAAGCTCAATCAAGAAAAGGTATATTATCCCAATTCAGTTTTATCCACGAAACCCATCAGCAATTATTACAGAAGTTCAAATATGGGCGACAGTGTGGAGTTCTCAATTGCTTTCACTACACCGGTGAAAAAGATAGGCGAGCTGAGAGGCAAAGTAAAGGAGTATGTGGAGGGGAATCTGACTCTTTGGCATCCAAACCACCAAATTGTGGTGCTTGAGATCGAAAATGTAAATAAGTTAAAGATGGCACTCTTTTTTAGTCACACGATGAACTTTCAAGAATGGGGAGAAAAGCAAAGGCGGAGAAGTGAAATGGTCATGGCATTGAAGAAATGTTTGGAGGACCTGCACATTACATACTATCTGCTGCCCCTAGAAGTTCGTCTCACTGAAACTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

752

Amino Acids

84.9

Weight (kDa)

9.28

Isoelectric Point (pI)

42.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	584 - 641	7.7e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2208
AccB1I	GGYRCC	1 cut(s)	1391
AccB7I	CCANNNNNTGG	2 cut(s)	1285, 2062
AccBSI	CCGCTC	1 cut(s)	1387
AccI	GTMKAC	1 cut(s)	1527
AciI	CCGC	9 cut(s)	9, 30, 255, 259, 464, 1070, 1385, 1789, 2155
AclWI	GGATC	3 cut(s)	244, 257, 1422
AcoI	YGGCCR	1 cut(s)	1457
AcsI	RAATTY	1 cut(s)	1017
AcuI	CTGAAG	3 cut(s)	1034, 1114, 1874
AfaI	GTAC	3 cut(s)	462, 1400, 1671
AfiI	CCNNNNNNNGG	7 cut(s)	233, 318, 383, 473, 477, 1285, 2062
AflII	CTTAAG	2 cut(s)	151, 971
AgeI	ACCGGT	1 cut(s)	1975
AhlI	ACTAGT	1 cut(s)	1253
AjnI	CCWGG	2 cut(s)	472, 1611
AjuI	GAANNNNNNNTTGG	2 cut(s)	2174, 2206
Alw21I	GWGCWC	1 cut(s)	1115
Alw26I	GTCTC	5 cut(s)	126, 619, 831, 875, 2243
AlwI	GGATC	3 cut(s)	244, 257, 1422
AoxI	GGCC	2 cut(s)	432, 1457
ApeKI	GCWGC	5 cut(s)	847, 1352, 1355, 1703, 2221
ApoI	RAATTY	1 cut(s)	1017
ArsI	GACNNNNNNTTYG	2 cut(s)	907, 939
AsiGI	ACCGGT	1 cut(s)	1975
Asp700I	GAANNNNTTC	1 cut(s)	1004
AspA2I	CCTAGG	1 cut(s)	245
AspS9I	GGNCC	5 cut(s)	432, 480, 1224, 1786, 2197
AsuC2I	CCSGG	1 cut(s)	234
AsuHPI	GGTGA	6 cut(s)	83, 195, 338, 697, 1098, 1990
AsuNHI	GCTAGC	1 cut(s)	608
AvaII	GGWCC	4 cut(s)	480, 1224, 1786, 2197
AvrII	CCTAGG	1 cut(s)	245
BalI	TGGCCA	1 cut(s)	1459
BamHI	GGATCC	1 cut(s)	249
BanI	GGYRCC	1 cut(s)	1391
BarI	GAAGNNNNNNTAC	2 cut(s)	1623, 1655
BbsI	GAAGAC	1 cut(s)	1840
Bbv12I	GWGCWC	1 cut(s)	1115
BbvI	GCAGC	5 cut(s)	834, 1339, 1342, 1715, 2208
BccI	CCATC	9 cut(s)	340, 1044, 1082, 1288, 1333, 1642, 1796, 1916, 2092
BceAI	ACGGC	2 cut(s)	451, 1562
BciT130I	CCWGG	2 cut(s)	474, 1613
BcnI	CCSGG	1 cut(s)	234
BcoDI	GTCTC	5 cut(s)	126, 619, 831, 875, 2243
BcuI	ACTAGT	1 cut(s)	1253
BfrI	CTTAAG	2 cut(s)	151, 971
BfuAI	ACCTGC	1 cut(s)	2208
BglII	AGATCT	1 cut(s)	975
BisI	GCNGC	7 cut(s)	9, 31, 848, 1353, 1356, 1704, 2222
BlnI	CCTAGG	1 cut(s)	245
BlsI	GCNGC	7 cut(s)	10, 32, 849, 1354, 1357, 1705, 2223
BmcAI	AGTACT	1 cut(s)	1671
Bme1390I	CCNGG	3 cut(s)	234, 474, 1613
Bme18I	GGWCC	4 cut(s)	480, 1224, 1786, 2197
BmgT120I	GGNCC	5 cut(s)	432, 480, 1224, 1786, 2197
BmiI	GGNNCC	3 cut(s)	251, 1393, 1787
BmrFI	CCNGG	3 cut(s)	234, 474, 1613
BmsI	GCATC	7 cut(s)	279, 992, 1075, 1234, 1755, 1777, 2053
BmtI	GCTAGC	1 cut(s)	612
BoxI	GACNNNNGTC	1 cut(s)	1607
BpiI	GAAGAC	1 cut(s)	1840
BpuEI	CTTGAG	1 cut(s)	2090
BpuMI	CCSGG	1 cut(s)	234
BsaBI	GATNNNNATC	1 cut(s)	1832
BsaI	GGTCTC	1 cut(s)	126
BsaJI	CCNNGG	4 cut(s)	232, 245, 472, 862
BsaWI	WCCGGW	3 cut(s)	205, 1481, 1975
BsaXI	ACNNNNNCTCC	4 cut(s)	60, 90, 1439, 1469
Bsc4I	CCNNNNNNNGG	7 cut(s)	233, 318, 383, 473, 477, 1285, 2062
Bse118I	RCCGGY	1 cut(s)	1975
Bse1I	ACTGG	3 cut(s)	171, 182, 1332
Bse3DI	GCAATG	1 cut(s)	268
Bse8I	GATNNNNATC	1 cut(s)	1832
BseBI	CCWGG	2 cut(s)	474, 1613
BseDI	CCNNGG	4 cut(s)	232, 245, 472, 862
BseGI	GGATG	8 cut(s)	214, 502, 957, 1066, 1249, 1294, 1768, 2044
BseJI	GATNNNNATC	1 cut(s)	1832
BseLI	CCNNNNNNNGG	7 cut(s)	233, 318, 383, 473, 477, 1285, 2062
BseMI	GCAATG	1 cut(s)	268
BseMII	CTCAG	3 cut(s)	156, 558, 1988
BseNI	ACTGG	3 cut(s)	171, 182, 1332
BseRI	GAGGAG	3 cut(s)	1094, 1421, 1838
BseXI	GCAGC	5 cut(s)	834, 1339, 1342, 1715, 2208
BseYI	CCCAGC	1 cut(s)	98
BsgI	GTGCAG	1 cut(s)	2186
BshFI	GGCC	2 cut(s)	434, 1459
BshNI	GGYRCC	1 cut(s)	1391
BshTI	ACCGGT	1 cut(s)	1975
BsiHKAI	GWGCWC	1 cut(s)	1115
BsiSI	CCGG	4 cut(s)	206, 234, 1482, 1976
BslFI	GGGAC	4 cut(s)	115, 210, 454, 1799
BslI	CCNNNNNNNGG	7 cut(s)	233, 318, 383, 473, 477, 1285, 2062
BsmAI	GTCTC	5 cut(s)	126, 619, 831, 875, 2243
BsmBI	CGTCTC	1 cut(s)	2243
BsmFI	GGGAC	4 cut(s)	115, 210, 454, 1799
BsnI	GGCC	2 cut(s)	434, 1459
Bso31I	GGTCTC	1 cut(s)	126
Bsp1286I	GDGCHC	1 cut(s)	1115
Bsp143I	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
BspACI	CCGC	9 cut(s)	9, 30, 255, 259, 464, 1070, 1385, 1789, 2155
BspANI	GGCC	2 cut(s)	434, 1459
BspCNI	CTCAG	3 cut(s)	157, 559, 1989
BspHI	TCATGA	1 cut(s)	1428
BspLI	GGNNCC	3 cut(s)	251, 1393, 1787
BspMI	ACCTGC	1 cut(s)	2208
BspOI	GCTAGC	1 cut(s)	612
BspPI	GGATC	3 cut(s)	244, 257, 1422
BspT107I	GGYRCC	1 cut(s)	1391
BspTI	CTTAAG	2 cut(s)	151, 971
BspTNI	GGTCTC	1 cut(s)	126
BsrBI	CCGCTC	1 cut(s)	1387
BsrDI	GCAATG	1 cut(s)	268
BsrFI	RCCGGY	1 cut(s)	1975
BsrI	ACTGG	3 cut(s)	171, 182, 1332
BssAI	RCCGGY	1 cut(s)	1975
BssECI	CCNNGG	4 cut(s)	232, 245, 472, 862
BssMI	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
BssT1I	CCWWGG	1 cut(s)	245
Bst2UI	CCWGG	2 cut(s)	474, 1613
Bst4CI	ACNGT	7 cut(s)	460, 559, 619, 863, 1609, 1846, 1946
Bst6I	CTCTTC	4 cut(s)	495, 617, 794, 1434
BstAFI	CTTAAG	2 cut(s)	151, 971
BstAPI	GCANNNNNTGC	1 cut(s)	1352
BstC8I	GCNNGC	3 cut(s)	259, 610, 1994
BstDEI	CTNAG	4 cut(s)	165, 567, 979, 1997
BstDSI	CCRYGG	1 cut(s)	862
BstF5I	GGATG	8 cut(s)	214, 502, 957, 1066, 1249, 1294, 1768, 2044
BstKTI	GATC	7 cut(s)	252, 364, 978, 1275, 1417, 1434, 2076
BstMAI	GTCTC	5 cut(s)	126, 619, 831, 875, 2243
BstMBI	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
BstMWI	GCNNNNNNNGC	5 cut(s)	39, 267, 1125, 1352, 1456
BstNI	CCWGG	2 cut(s)	474, 1613
BstNSI	RCATGY	2 cut(s)	46, 703
BstPAI	GACNNNNGTC	1 cut(s)	1607
BstSCI	CCNGG	3 cut(s)	232, 472, 1611
BstV1I	GCAGC	5 cut(s)	834, 1339, 1342, 1715, 2208
BstV2I	GAAGAC	1 cut(s)	1840
BstX2I	RGATCY	3 cut(s)	249, 975, 1414
BstXI	CCANNNNNNTGG	2 cut(s)	98, 1339
BstYI	RGATCY	3 cut(s)	249, 975, 1414
BsuRI	GGCC	2 cut(s)	434, 1459
BtgI	CCRYGG	1 cut(s)	862
BtgZI	GCGATG	1 cut(s)	318
BtsCI	GGATG	8 cut(s)	214, 502, 957, 1066, 1249, 1294, 1768, 2044
BtsIMutI	CAGTG	5 cut(s)	162, 700, 1339, 1951, 2241
BveI	ACCTGC	1 cut(s)	2208
Cac8I	GCNNGC	3 cut(s)	259, 610, 1994
CciI	TCATGA	1 cut(s)	1428
Cfr10I	RCCGGY	1 cut(s)	1975
Cfr13I	GGNCC	5 cut(s)	432, 480, 1224, 1786, 2197
Csp6I	GTAC	3 cut(s)	461, 1399, 1670
CspAI	ACCGGT	1 cut(s)	1975
CviQI	GTAC	3 cut(s)	461, 1399, 1670
DdeI	CTNAG	4 cut(s)	165, 567, 979, 1997
DpnI	GATC	7 cut(s)	251, 363, 977, 1274, 1416, 1433, 2075
DpnII	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
DraI	TTTAAA	1 cut(s)	900
EaeI	YGGCCR	1 cut(s)	1457
Eam1104I	CTCTTC	4 cut(s)	495, 617, 794, 1434
EarI	CTCTTC	4 cut(s)	495, 617, 794, 1434
EciI	GGCGGA	1 cut(s)	2170
Eco130I	CCWWGG	1 cut(s)	245
Eco31I	GGTCTC	1 cut(s)	126
Eco47I	GGWCC	4 cut(s)	480, 1224, 1786, 2197
Eco57I	CTGAAG	3 cut(s)	1034, 1114, 1874
EcoO109I	RGGNCCY	1 cut(s)	2197
EcoRI	GAATTC	1 cut(s)	1017
EcoRII	CCWGG	2 cut(s)	472, 1611
EcoT14I	CCWWGG	1 cut(s)	245
EcoT22I	ATGCAT	2 cut(s)	726, 1770
ErhI	CCWWGG	1 cut(s)	245
Esp3I	CGTCTC	1 cut(s)	2243
FalI	AAGNNNNNCTT	2 cut(s)	545, 577
FaqI	GGGAC	4 cut(s)	115, 210, 454, 1799
FauI	CCCGC	2 cut(s)	262, 266
FblI	GTMKAC	1 cut(s)	1527
Fnu4HI	GCNGC	7 cut(s)	9, 31, 848, 1353, 1356, 1704, 2222
FokI	GGATG	8 cut(s)	221, 509, 964, 1053, 1256, 1301, 1755, 2031
Fsp4HI	GCNGC	7 cut(s)	9, 31, 848, 1353, 1356, 1704, 2222
GluI	GCNGC	7 cut(s)	9, 31, 848, 1353, 1356, 1704, 2222
GsaI	CCCAGC	1 cut(s)	102
HaeIII	GGCC	2 cut(s)	434, 1459
HapII	CCGG	4 cut(s)	206, 234, 1482, 1976
HindIII	AAGCTT	1 cut(s)	1187
HinfI	GANTC	5 cut(s)	156, 167, 1025, 2029, 2035
HpaII	CCGG	4 cut(s)	206, 234, 1482, 1976
HphI	GGTGA	6 cut(s)	83, 195, 338, 697, 1098, 1990
Hpy166II	GTNNAC	6 cut(s)	148, 308, 329, 1000, 1269, 1528
Hpy188I	TCNGA	8 cut(s)	49, 361, 765, 880, 1053, 1133, 1305, 2034
Hpy188III	TCNNGA	7 cut(s)	423, 562, 1022, 1429, 1852, 1865, 2132
Hpy8I	GTNNAC	6 cut(s)	148, 308, 329, 1000, 1269, 1528
Hpy99I	CGWCG	1 cut(s)	1382
HpyCH4III	ACNGT	7 cut(s)	460, 559, 619, 863, 1609, 1846, 1946
HpyCH4IV	ACGT	1 cut(s)	1377
HpyCH4V	TGCA	9 cut(s)	62, 588, 724, 750, 1247, 1658, 1728, 1768, 2203
HpyF10VI	GCNNNNNNNGC	5 cut(s)	39, 267, 1125, 1352, 1456
HpyF3I	CTNAG	4 cut(s)	165, 567, 979, 1997
HpySE526I	ACGT	1 cut(s)	1377
Kzo9I	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
LmnI	GCTCC	3 cut(s)	425, 1387, 1447
Lsp1109I	GCAGC	5 cut(s)	834, 1339, 1342, 1715, 2208
LweI	GCATC	7 cut(s)	279, 992, 1075, 1234, 1755, 1777, 2053
MaeII	ACGT	1 cut(s)	1377
MaeIII	GTNAC	5 cut(s)	706, 907, 1249, 1600, 2114
MalI	GATC	7 cut(s)	251, 363, 977, 1274, 1416, 1433, 2075
MbiI	CCGCTC	1 cut(s)	1387
MboI	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
MfeI	CAATTG	2 cut(s)	1586, 1959
MflI	RGATCY	3 cut(s)	249, 975, 1414
MhlI	GDGCHC	1 cut(s)	1115
MlsI	TGGCCA	1 cut(s)	1459
MluNI	TGGCCA	1 cut(s)	1459
MlyI	GAGTC	3 cut(s)	150, 176, 2029
MmeI	TCCRAC	1 cut(s)	27
Mox20I	TGGCCA	1 cut(s)	1459
Mph1103I	ATGCAT	2 cut(s)	726, 1770
MroXI	GAANNNNTTC	1 cut(s)	1004
MscI	TGGCCA	1 cut(s)	1459
MslI	CAYNNNNRTG	2 cut(s)	1041, 1178
Msp20I	TGGCCA	1 cut(s)	1459
MspA1I	CMGCKG	1 cut(s)	1791
MspCI	CTTAAG	2 cut(s)	151, 971
MspI	CCGG	4 cut(s)	206, 234, 1482, 1976
MspR9I	CCNGG	3 cut(s)	234, 474, 1613
MunI	CAATTG	2 cut(s)	1586, 1959
MvaI	CCWGG	2 cut(s)	474, 1613
MwoI	GCNNNNNNNGC	5 cut(s)	39, 267, 1125, 1352, 1456
NciI	CCSGG	1 cut(s)	234
NdeII	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
NheI	GCTAGC	1 cut(s)	608
NlaIV	GGNNCC	3 cut(s)	251, 1393, 1787
NmuCI	GTSAC	2 cut(s)	1600, 2114
NsiI	ATGCAT	2 cut(s)	726, 1770
NspI	RCATGY	2 cut(s)	46, 703
PagI	TCATGA	1 cut(s)	1428
PdmI	GAANNNNTTC	1 cut(s)	1004
PfeI	GAWTC	2 cut(s)	1025, 2029
PflMI	CCANNNNNTGG	2 cut(s)	1285, 2062
PinAI	ACCGGT	1 cut(s)	1975
PkrI	GCNGC	7 cut(s)	10, 32, 849, 1354, 1357, 1705, 2223
PleI	GAGTC	3 cut(s)	150, 175, 2029
PpsI	GAGTC	3 cut(s)	150, 175, 2029
PpuMI	RGGWCCY	1 cut(s)	2197
PshAI	GACNNNNGTC	1 cut(s)	1607
Psp5II	RGGWCCY	1 cut(s)	2197
Psp6I	CCWGG	2 cut(s)	472, 1611
PspFI	CCCAGC	1 cut(s)	98
PspGI	CCWGG	2 cut(s)	472, 1611
PspN4I	GGNNCC	3 cut(s)	251, 1393, 1787
PspPI	GGNCC	5 cut(s)	432, 480, 1224, 1786, 2197
PspPPI	RGGWCCY	1 cut(s)	2197
PsuI	RGATCY	3 cut(s)	249, 975, 1414
RsaI	GTAC	3 cut(s)	462, 1400, 1671
RsaNI	GTAC	3 cut(s)	461, 1399, 1670
RseI	CAYNNNNRTG	2 cut(s)	1041, 1178
SatI	GCNGC	7 cut(s)	9, 31, 848, 1353, 1356, 1704, 2222
Sau3AI	GATC	7 cut(s)	249, 361, 975, 1272, 1414, 1431, 2073
Sau96I	GGNCC	5 cut(s)	432, 480, 1224, 1786, 2197
ScaI	AGTACT	1 cut(s)	1671
SchI	GAGTC	3 cut(s)	150, 176, 2029
ScrFI	CCNGG	3 cut(s)	234, 474, 1613
SduI	GDGCHC	1 cut(s)	1115
SfaNI	GCATC	7 cut(s)	279, 992, 1075, 1234, 1755, 1777, 2053
SgrAI	CRCCGGYG	1 cut(s)	1975
SinI	GGWCC	4 cut(s)	480, 1224, 1786, 2197
SmiMI	CAYNNNNRTG	2 cut(s)	1041, 1178
SmlI	CTYRAG	3 cut(s)	151, 971, 2069
SmoI	CTYRAG	3 cut(s)	151, 971, 2069
SpeI	ACTAGT	1 cut(s)	1253
SsiI	CCGC	9 cut(s)	9, 30, 255, 259, 464, 1070, 1385, 1789, 2155
StyD4I	CCNGG	3 cut(s)	232, 472, 1611
StyI	CCWWGG	1 cut(s)	245
TaaI	ACNGT	7 cut(s)	460, 559, 619, 863, 1609, 1846, 1946
TaiI	ACGT	1 cut(s)	1380
TaqI	TCGA	4 cut(s)	188, 1434, 1800, 2076
TatI	WGTACW	2 cut(s)	1398, 1669
TauI	GCSGC	2 cut(s)	11, 33
TfiI	GAWTC	2 cut(s)	1025, 2029
TscAI	CASTG	5 cut(s)	169, 700, 1339, 1951, 2248
TseFI	GTSAC	2 cut(s)	1600, 2114
TseI	GCWGC	5 cut(s)	847, 1352, 1355, 1703, 2221
Tsp45I	GTSAC	2 cut(s)	1600, 2114
TspGWI	ACGGA	1 cut(s)	1577
TspRI	CASTG	5 cut(s)	169, 700, 1339, 1951, 2248
Van91I	CCANNNNNTGG	2 cut(s)	1285, 2062
Vha464I	CTTAAG	2 cut(s)	151, 971
VpaK11BI	GGWCC	4 cut(s)	480, 1224, 1786, 2197
XapI	RAATTY	1 cut(s)	1017
XceI	RCATGY	2 cut(s)	46, 703
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1234
XmaJI	CCTAGG	1 cut(s)	245
XmiI	GTMKAC	1 cut(s)	1527
XmnI	GAANNNNTTC	1 cut(s)	1004
ZrmI	AGTACT	1 cut(s)	1671
Zsp2I	ATGCAT	2 cut(s)	726, 1770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.