Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Forward (+)

68436759 .. 68439871

Gene Structure 3113 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ28021

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGAACCAAAAGGCTCAGACTCTTCAGCTAGCCAGACCCTCACTAAGAGAAAGTCATTCAGTAGGTCAGTTCTCTCCAAACCCAAGTCGAGATTCGGTGAACAATTTCCCATTATGTATGAAGACTGAAGAGACTAGTTTAGAACAGGCAGCTGATGCAAATTCCCCCAATGCTTCATTCAATAGGGAGTCCCCGAATGAAATCTCATTGGTTAGGACTGAGGAGAATGCACCGCCATCTCCAGGCAAAACAAAAGATGAGGAGGATGTGGAGATTTACAAGAAGGTTAAATTGAGTAGAAGAGTGAGCTGCAAAAAAATCTCGATTGAGTGGATTGTTTTTCTATCCATTTTGGGTTTTTTAGTGGCTAGCTTGACTGTCCATAAGCTAAAGAATCTTATGGTTTGGGGATTGGAGGTTTGGAAATGGTGTGCGCTTATAATGGTGGTATTATGTGGCATGCTGATGACAAATTGGGTTATCGGTTTCATATTTTTGGTGATCGAGCGCAACTTCTTGCTTAGGAAGAAGGTGTTGTATTTTGTTTATGGAATGAAGACGGGTATTCAGTTTCTCATGTGGGGGAGTCTGATTCTTCTCACATGGCTACTCTTGATCAAACGCGGGCTTCAGCAATCAAAAGTTTCCACTAAGATTCTGGCTTACATCACGCGAAGTATCGTTGCAGTTGTTATTGGGGCATTTTTGTGGTTGTTAAAGACTTTGTTGGTTCTGATCTTAGCATCTAAATTCGAGGCGGTCACCTATTTTGATAGAATTCAAGAATCGATATTCAATCAATATGTTCTTCAGACACTTTCAGGACCTCCGTTGATGGAGGAGGCTTATGAAATATCACGAGGCAGTAGCAAACAAAGTTACAGAGAGACTAGGAAGGCACAGGCCGAACGGGTGGAGAAAAGGGTCGTCATTGACATGACAAAGCTTCAAAGGATAAAGCAAGAGAAGGTTTCTTCTTGGACCTTGAAGGTATTGATGGATGCAGTAACTAGTTCAGGGCTATCCACCATCTCTCATACATTGGACGGGATAGAGGATCTAAATGAACAGAGAGATAAAGAGATCACCAGTGAGATGGAAGCAACTGCTGCTGCACATGACATTTTCCTTAACGTCGCTCCGCCTGGTTCTAAGTACATTGAGGAGTGGGATCTCTTGAGGTTCATGATCCAGGAAGAGGTAGAGTTTGTATGGCCGCTGATTGACGTAGCAATGACCGGACAAGTTGACAGAAAAACTCTGACAGACTGGGTGGTGAAGCTCTACACTGATCGCAAAGCATTAGCTCATGCATTGTCTGATACCAAAGCAGTTACAGCGCAAGTCAACAATCTTGTGACTAGCATTGTGTTTATTATCATCATCGTTGTGAGCCTTCTAGTGGTGGAAATTGCAACCACAAGCTTTTTTGTCTTCCTATCATCACAGCTCGTATTAGCAGCTTTTGCTTTCGGAAACACTTGCAAGAATGTATTTGAATCTATCATGTTTCTATTTGTGGCACATCCATATGATGTTGGTGACTGCATTGTTTTTGAAGGCGTTCCGATGATAGTTGAAGAGATGAACATCTTTACTACGGTTTTCTTGAAACTCAGTAATAACGAGAAGGTGTACTATCCGAATTCAGTTTTATCTGCAACATTGATCAGCAATTACTCCAGAAGTCCAGATATGAGAGATGCTGTAGAATTCTCAATTGCTTTAACACCAGTGGAGAAGATAGTCAAGTTGAAAGAAAAAATAAAGGAATACATCGAGGGGAAGCCTCAATATTGGCATCGAAAACACAATGTGGTGGTGATTAACATCGAAGATGTTGTTAAGTTAAAGATGGCAGTCTATTGTCATCACAAAATGAACTATAAAGATTTTGAGGAAAAGAATTTGCGCAGAGCTGAGCTAAACTTCGAATTGCTGAAAATTCTTGAAGAGCTAAACATCACAGTAATATCTGCTGACCTCCCAAAAAGTGAATGTCACTCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

670

Amino Acids

76.5

Weight (kDa)

7.57

Isoelectric Point (pI)

40.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	494 - 562	2.5e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	440
Acc16I	TGCGCA	1 cut(s)	1913
AccII	CGCG	2 cut(s)	624, 673
AciI	CCGC	5 cut(s)	233, 624, 758, 1142, 1217
AclWI	GGATC	3 cut(s)	1065, 1179, 1183
AcoI	YGGCCR	1 cut(s)	1214
AcsI	RAATTY	7 cut(s)	160, 749, 777, 1645, 1712, 1906, 1944
AcuI	CTGAAG	4 cut(s)	8, 147, 614, 794
AdeI	CACNNNGTG	1 cut(s)	1816
AfaI	GTAC	2 cut(s)	1157, 1637
AfiI	CCNNNNNNNGG	3 cut(s)	242, 1198, 1402
AhlI	ACTAGT	2 cut(s)	134, 1010
AjnI	CCWGG	3 cut(s)	241, 1144, 1191
AjuI	GAANNNNNNNTTGG	4 cut(s)	76, 108, 161, 193
Alw26I	GTCTC	2 cut(s)	125, 881
AlwI	GGATC	3 cut(s)	1065, 1179, 1183
AoxI	GGCC	2 cut(s)	903, 1214
ApeKI	GCWGC	5 cut(s)	149, 309, 1109, 1112, 1460
ApoI	RAATTY	7 cut(s)	160, 749, 777, 1645, 1712, 1906, 1944
Asp700I	GAANNNNTTC	2 cut(s)	104, 1563
AspLEI	GCGC	4 cut(s)	436, 510, 1342, 1914
AspS9I	GGNCC	2 cut(s)	824, 981
AsuHPI	GGTGA	7 cut(s)	109, 511, 754, 1078, 1288, 1553, 1834
AsuII	TTCGAA	1 cut(s)	1932
AsuNHI	GCTAGC	2 cut(s)	28, 368
AvaII	GGWCC	2 cut(s)	824, 981
BauI	CACGAG	1 cut(s)	858
BbsI	GAAGAC	3 cut(s)	128, 563, 1426
BbvI	GCAGC	5 cut(s)	161, 296, 1096, 1099, 1472
BccI	CCATC	6 cut(s)	244, 829, 991, 1037, 1090, 1849
BciT130I	CCWGG	3 cut(s)	243, 1146, 1193
BclI	TGATCA	2 cut(s)	615, 1668
BcoDI	GTCTC	2 cut(s)	125, 881
BcuI	ACTAGT	2 cut(s)	134, 1010
BfaI	CTAG	7 cut(s)	29, 135, 369, 891, 1011, 1362, 1400
BfmI	CTRYAG	1 cut(s)	1707
BisI	GCNGC	6 cut(s)	150, 310, 1110, 1113, 1217, 1461
BlpI	GCTNAGC	1 cut(s)	1920
BlsI	GCNGC	6 cut(s)	151, 311, 1111, 1114, 1218, 1462
Bme1390I	CCNGG	3 cut(s)	243, 1146, 1193
Bme18I	GGWCC	2 cut(s)	824, 981
BmgT120I	GGNCC	2 cut(s)	824, 981
BmrFI	CCNGG	3 cut(s)	243, 1146, 1193
BmrI	ACTGGG	1 cut(s)	1279
BmsI	GCATC	5 cut(s)	145, 752, 991, 1693, 1810
BmtI	GCTAGC	2 cut(s)	32, 372
BmuI	ACTGGG	1 cut(s)	1279
BpiI	GAAGAC	3 cut(s)	128, 563, 1426
BpmI	CTGGAG	2 cut(s)	225, 1666
Bpu10I	CCTNAGC	1 cut(s)	521
Bpu1102I	GCTNAGC	1 cut(s)	1920
Bpu14I	TTCGAA	1 cut(s)	1932
BpuEI	CTTGAG	1 cut(s)	1198
Bsa29I	ATCGAT	1 cut(s)	788
BsaBI	GATNNNNATC	2 cut(s)	1589, 1829
BsaWI	WCCGGW	1 cut(s)	1238
Bsc4I	CCNNNNNNNGG	3 cut(s)	242, 1198, 1402
Bse1I	ACTGG	3 cut(s)	1089, 1274, 1733
Bse3DI	GCAATG	1 cut(s)	1239
Bse8I	GATNNNNATC	2 cut(s)	1589, 1829
BseBI	CCWGG	3 cut(s)	243, 1146, 1193
BseCI	ATCGAT	1 cut(s)	788
BseGI	GGATG	3 cut(s)	271, 1006, 1525
BseJI	GATNNNNATC	2 cut(s)	1589, 1829
BseLI	CCNNNNNNNGG	3 cut(s)	242, 1198, 1402
BseMI	GCAATG	1 cut(s)	1239
BseMII	CTCAG	4 cut(s)	29, 210, 1630, 1911
BseNI	ACTGG	3 cut(s)	1089, 1274, 1733
BseRI	GAGGAG	4 cut(s)	236, 275, 854, 1178
BseXI	GCAGC	5 cut(s)	161, 296, 1096, 1099, 1472
BsgI	GTGCAG	1 cut(s)	1098
Bsh1236I	CGCG	2 cut(s)	624, 673
BshFI	GGCC	2 cut(s)	905, 1216
BshVI	ATCGAT	1 cut(s)	788
BsiSI	CCGG	1 cut(s)	1239
BslFI	GGGAC	1 cut(s)	175
BslI	CCNNNNNNNGG	3 cut(s)	242, 1198, 1402
BsmAI	GTCTC	2 cut(s)	125, 881
BsmFI	GGGAC	1 cut(s)	175
BsmI	GAATGC	1 cut(s)	232
BsnI	GGCC	2 cut(s)	905, 1216
Bsp119I	TTCGAA	1 cut(s)	1932
Bsp143I	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
Bsp1720I	GCTNAGC	1 cut(s)	1920
BspACI	CCGC	5 cut(s)	233, 624, 758, 1142, 1217
BspANI	GGCC	2 cut(s)	905, 1216
BspCNI	CTCAG	4 cut(s)	28, 211, 1629, 1912
BspDI	ATCGAT	1 cut(s)	788
BspFNI	CGCG	2 cut(s)	624, 673
BspHI	TCATGA	1 cut(s)	1185
BspOI	GCTAGC	2 cut(s)	32, 372
BspPI	GGATC	3 cut(s)	1065, 1179, 1183
BspQI	GCTCTTC	1 cut(s)	1947
BspT104I	TTCGAA	1 cut(s)	1932
BsrDI	GCAATG	1 cut(s)	1239
BsrI	ACTGG	3 cut(s)	1089, 1274, 1733
BssMI	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
BssSI	CACGAG	1 cut(s)	858
Bst2BI	CACGAG	1 cut(s)	858
Bst2UI	CCWGG	3 cut(s)	243, 1146, 1193
Bst4CI	ACNGT	3 cut(s)	379, 1603, 1969
Bst6I	CTCTTC	6 cut(s)	27, 123, 295, 1191, 1575, 1947
BstAPI	GCANNNNNTGC	3 cut(s)	155, 1109, 1466
BstBI	TTCGAA	1 cut(s)	1932
BstC8I	GCNNGC	4 cut(s)	30, 370, 461, 626
BstDEI	CTNAG	9 cut(s)	15, 44, 219, 521, 651, 739, 1152, 1616, 1920
BstEII	GGTNACC	1 cut(s)	760
BstF5I	GGATG	3 cut(s)	271, 1006, 1525
BstFNI	CGCG	2 cut(s)	624, 673
BstHHI	GCGC	4 cut(s)	436, 510, 1342, 1914
BstKTI	GATC	9 cut(s)	504, 618, 738, 1060, 1086, 1174, 1191, 1294, 1671
BstMAI	GTCTC	2 cut(s)	125, 881
BstMBI	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
BstMWI	GCNNNNNNNGC	4 cut(s)	155, 1109, 1337, 1466
BstNI	CCWGG	3 cut(s)	243, 1146, 1193
BstNSI	RCATGY	1 cut(s)	463
BstPI	GGTNACC	1 cut(s)	760
BstSCI	CCNGG	3 cut(s)	241, 1144, 1191
BstSFI	CTRYAG	1 cut(s)	1707
BstUI	CGCG	2 cut(s)	624, 673
BstV1I	GCAGC	5 cut(s)	161, 296, 1096, 1099, 1472
BstV2I	GAAGAC	3 cut(s)	128, 563, 1426
BstX2I	RGATCY	2 cut(s)	1057, 1171
BstXI	CCANNNNNNTGG	1 cut(s)	1096
BstYI	RGATCY	2 cut(s)	1057, 1171
Bsu15I	ATCGAT	1 cut(s)	788
BsuRI	GGCC	2 cut(s)	905, 1216
BsuTUI	ATCGAT	1 cut(s)	788
BtsCI	GGATG	3 cut(s)	271, 1006, 1525
BtsIMutI	CAGTG	3 cut(s)	1096, 1287, 1740
Cac8I	GCNNGC	4 cut(s)	30, 370, 461, 626
CciI	TCATGA	1 cut(s)	1185
CfoI	GCGC	4 cut(s)	436, 510, 1342, 1914
Cfr13I	GGNCC	2 cut(s)	824, 981
ClaI	ATCGAT	1 cut(s)	788
Csp6I	GTAC	2 cut(s)	1156, 1636
CviAII	CATG	8 cut(s)	460, 577, 603, 937, 1118, 1186, 1310, 1507
CviQI	GTAC	2 cut(s)	1156, 1636
DdeI	CTNAG	9 cut(s)	15, 44, 219, 521, 651, 739, 1152, 1616, 1920
DpnI	GATC	9 cut(s)	503, 617, 737, 1059, 1085, 1173, 1190, 1293, 1670
DpnII	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
DraIII	CACNNNGTG	1 cut(s)	1816
EaeI	YGGCCR	1 cut(s)	1214
Eam1104I	CTCTTC	6 cut(s)	27, 123, 295, 1191, 1575, 1947
EarI	CTCTTC	6 cut(s)	27, 123, 295, 1191, 1575, 1947
EciI	GGCGGA	1 cut(s)	1131
Eco47I	GGWCC	2 cut(s)	824, 981
Eco57I	CTGAAG	4 cut(s)	8, 147, 614, 794
Eco91I	GGTNACC	1 cut(s)	760
EcoO109I	RGGNCCY	1 cut(s)	824
EcoO65I	GGTNACC	1 cut(s)	760
EcoRI	GAATTC	3 cut(s)	777, 1645, 1712
EcoRII	CCWGG	3 cut(s)	241, 1144, 1191
EcoT22I	ATGCAT	1 cut(s)	1315
FaeI	CATG	8 cut(s)	463, 580, 606, 940, 1121, 1189, 1313, 1510
FaqI	GGGAC	1 cut(s)	175
FatI	CATG	8 cut(s)	459, 576, 602, 936, 1117, 1185, 1309, 1506
FauI	CCCGC	1 cut(s)	617
FauNDI	CATATG	1 cut(s)	1531
FbaI	TGATCA	2 cut(s)	615, 1668
Fnu4HI	GCNGC	6 cut(s)	150, 310, 1110, 1113, 1217, 1461
FokI	GGATG	3 cut(s)	278, 1013, 1512
Fsp4HI	GCNGC	6 cut(s)	150, 310, 1110, 1113, 1217, 1461
FspBI	CTAG	7 cut(s)	29, 135, 369, 891, 1011, 1362, 1400
FspI	TGCGCA	1 cut(s)	1913
GlaI	GCGC	4 cut(s)	435, 509, 1341, 1913
GluI	GCNGC	6 cut(s)	150, 310, 1110, 1113, 1217, 1461
GsuI	CTGGAG	2 cut(s)	225, 1666
HaeIII	GGCC	2 cut(s)	905, 1216
HapII	CCGG	1 cut(s)	1239
HhaI	GCGC	4 cut(s)	436, 510, 1342, 1914
Hin1II	CATG	8 cut(s)	463, 580, 606, 940, 1121, 1189, 1313, 1510
Hin6I	GCGC	4 cut(s)	434, 508, 1340, 1912
HinP1I	GCGC	4 cut(s)	434, 508, 1340, 1912
HincII	GTYRAC	2 cut(s)	1249, 1348
HindII	GTYRAC	2 cut(s)	1249, 1348
HindIII	AAGCTT	2 cut(s)	944, 1423
HinfI	GANTC	9 cut(s)	19, 92, 188, 394, 586, 592, 655, 785, 1499
HpaII	CCGG	1 cut(s)	1239
HphI	GGTGA	7 cut(s)	109, 511, 754, 1078, 1288, 1553, 1834
Hpy166II	GTNNAC	4 cut(s)	100, 1249, 1348, 1636
Hpy188I	TCNGA	9 cut(s)	18, 591, 735, 813, 1263, 1321, 1475, 1569, 1644
Hpy8I	GTNNAC	4 cut(s)	100, 1249, 1348, 1636
Hpy99I	CGWCG	1 cut(s)	1139
HpyAV	CCTTC	8 cut(s)	277, 523, 889, 961, 982, 1406, 1553, 1624
HpyCH4III	ACNGT	3 cut(s)	379, 1603, 1969
HpyCH4IV	ACGT	2 cut(s)	1134, 1227
HpyF10VI	GCNNNNNNNGC	4 cut(s)	155, 1109, 1337, 1466
HpyF3I	CTNAG	9 cut(s)	15, 44, 219, 521, 651, 739, 1152, 1616, 1920
HpySE526I	ACGT	2 cut(s)	1134, 1227
Hsp92II	CATG	8 cut(s)	463, 580, 606, 940, 1121, 1189, 1313, 1510
HspAI	GCGC	4 cut(s)	434, 508, 1340, 1912
Ksp22I	TGATCA	2 cut(s)	615, 1668
Kzo9I	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
LguI	GCTCTTC	1 cut(s)	1947
LmnI	GCTCC	1 cut(s)	1144
Lsp1109I	GCAGC	5 cut(s)	161, 296, 1096, 1099, 1472
LweI	GCATC	5 cut(s)	145, 752, 991, 1693, 1810
MaeI	CTAG	7 cut(s)	29, 135, 369, 891, 1011, 1362, 1400
MaeII	ACGT	2 cut(s)	1134, 1227
MaeIII	GTNAC	7 cut(s)	760, 878, 1006, 1333, 1357, 1541, 2000
MalI	GATC	9 cut(s)	503, 617, 737, 1059, 1085, 1173, 1190, 1293, 1670
MboI	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
MfeI	CAATTG	1 cut(s)	1719
MflI	RGATCY	2 cut(s)	1057, 1171
MlyI	GAGTC	3 cut(s)	13, 197, 595
Mph1103I	ATGCAT	1 cut(s)	1315
MroXI	GAANNNNTTC	2 cut(s)	104, 1563
MseI	TTAA	7 cut(s)	288, 716, 1131, 1727, 1827, 1845, 1850
MslI	CAYNNNNRTG	5 cut(s)	464, 706, 935, 1388, 1530
MspA1I	CMGCKG	2 cut(s)	152, 1219
MspI	CCGG	1 cut(s)	1239
MspR9I	CCNGG	3 cut(s)	243, 1146, 1193
MunI	CAATTG	1 cut(s)	1719
Mva1269I	GAATGC	1 cut(s)	232
MvaI	CCWGG	3 cut(s)	243, 1146, 1193
MvnI	CGCG	2 cut(s)	624, 673
MwoI	GCNNNNNNNGC	4 cut(s)	155, 1109, 1337, 1466
NdeI	CATATG	1 cut(s)	1531
NdeII	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
NheI	GCTAGC	2 cut(s)	28, 368
NlaIII	CATG	8 cut(s)	463, 580, 606, 940, 1121, 1189, 1313, 1510
NmuCI	GTSAC	4 cut(s)	760, 1357, 1541, 2000
NsbI	TGCGCA	1 cut(s)	1913
NsiI	ATGCAT	1 cut(s)	1315
NspI	RCATGY	1 cut(s)	463
NspV	TTCGAA	1 cut(s)	1932
PaeI	GCATGC	1 cut(s)	463
PagI	TCATGA	1 cut(s)	1185
PciSI	GCTCTTC	1 cut(s)	1947
PctI	GAATGC	1 cut(s)	232
PdmI	GAANNNNTTC	2 cut(s)	104, 1563
PfeI	GAWTC	6 cut(s)	92, 394, 592, 655, 785, 1499
PfoI	TCCNGGA	1 cut(s)	1191
PkrI	GCNGC	6 cut(s)	151, 311, 1111, 1114, 1218, 1462
PleI	GAGTC	3 cut(s)	13, 196, 594
PpsI	GAGTC	3 cut(s)	13, 196, 594
PpuMI	RGGWCCY	1 cut(s)	824
PsiI	TTATAA	1 cut(s)	440
Psp5II	RGGWCCY	1 cut(s)	824
Psp6I	CCWGG	3 cut(s)	241, 1144, 1191
PspEI	GGTNACC	1 cut(s)	760
PspGI	CCWGG	3 cut(s)	241, 1144, 1191
PspPI	GGNCC	2 cut(s)	824, 981
PspPPI	RGGWCCY	1 cut(s)	824
PsuI	RGATCY	2 cut(s)	1057, 1171
PvuII	CAGCTG	1 cut(s)	152
RsaI	GTAC	2 cut(s)	1157, 1637
RsaNI	GTAC	2 cut(s)	1156, 1636
RseI	CAYNNNNRTG	5 cut(s)	464, 706, 935, 1388, 1530
SapI	GCTCTTC	1 cut(s)	1947
SaqAI	TTAA	7 cut(s)	288, 716, 1131, 1727, 1827, 1845, 1850
SatI	GCNGC	6 cut(s)	150, 310, 1110, 1113, 1217, 1461
Sau3AI	GATC	9 cut(s)	501, 615, 735, 1057, 1083, 1171, 1188, 1291, 1668
Sau96I	GGNCC	2 cut(s)	824, 981
SchI	GAGTC	3 cut(s)	13, 197, 595
ScrFI	CCNGG	3 cut(s)	243, 1146, 1193
SfaNI	GCATC	5 cut(s)	145, 752, 991, 1693, 1810
SfcI	CTRYAG	1 cut(s)	1707
SfuI	TTCGAA	1 cut(s)	1932
SinI	GGWCC	2 cut(s)	824, 981
SmiMI	CAYNNNNRTG	5 cut(s)	464, 706, 935, 1388, 1530
SmlI	CTYRAG	1 cut(s)	1177
SmoI	CTYRAG	1 cut(s)	1177
SpeI	ACTAGT	2 cut(s)	134, 1010
SphI	GCATGC	1 cut(s)	463
SsiI	CCGC	5 cut(s)	233, 624, 758, 1142, 1217
SspI	AATATT	1 cut(s)	1796
SspMI	CTAG	7 cut(s)	29, 135, 369, 891, 1011, 1362, 1400
StyD4I	CCNGG	3 cut(s)	241, 1144, 1191
TaaI	ACNGT	3 cut(s)	379, 1603, 1969
TaiI	ACGT	2 cut(s)	1137, 1230
TaqI	TCGA	9 cut(s)	88, 323, 504, 753, 788, 1779, 1804, 1833, 1932
TatI	WGTACW	2 cut(s)	1155, 1635
TauI	GCSGC	1 cut(s)	1219
TfiI	GAWTC	6 cut(s)	92, 394, 592, 655, 785, 1499
Tru1I	TTAA	7 cut(s)	288, 716, 1131, 1727, 1827, 1845, 1850
Tru9I	TTAA	7 cut(s)	288, 716, 1131, 1727, 1827, 1845, 1850
TscAI	CASTG	3 cut(s)	1096, 1294, 1740
TseFI	GTSAC	4 cut(s)	760, 1357, 1541, 2000
TseI	GCWGC	5 cut(s)	149, 309, 1109, 1112, 1460
Tsp45I	GTSAC	4 cut(s)	760, 1357, 1541, 2000
TspGWI	ACGGA	1 cut(s)	819
TspRI	CASTG	3 cut(s)	1096, 1294, 1740
VpaK11BI	GGWCC	2 cut(s)	824, 981
XapI	RAATTY	7 cut(s)	160, 749, 777, 1645, 1712, 1906, 1944
XceI	RCATGY	1 cut(s)	463
XcmI	CCANNNNNNNNNTGG	1 cut(s)	655
XmnI	GAANNNNTTC	2 cut(s)	104, 1563
XspI	CTAG	7 cut(s)	29, 135, 369, 891, 1011, 1362, 1400
Zsp2I	ATGCAT	1 cut(s)	1315

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.