Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

3577319 .. 3581665

Gene Structure 4347 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1052100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGAAGGCCATAGAGAGAGAAAAGCTTTTGAAAGGCGGGGAAGTAAGCATGTCGGAGAAGCTTTCTTCCAATGGAGGTGAAGTTGTACTGGAAGTTCGAAGCGAAGAGATCAATGCAGCAAAAGGATCTCCCTCAAAAGGCTCGGGATATTCAGCTCCCAAGCAAAGCAAAGTGGGCTCACCCGCCAAAGCATCGGCTGAGTCCAGCAGTGAGTTCTCTAAGTCGGTCCCAGTGAGCTCCCCTTCACCTGAATTTTCGAGAGCCAGTCCTGGTAAGCCTCCCAAGATTCCCACCCGCAATGAGAGTCTTGCTAGGAGAAAGTCACTAAATAGGTCAACTAACTCGAAACCCAAGTCAAGATTTGGTGAACCCTCAGTAGGCATTGATCACAATATGTTTGAAGAAGATCAAGTTGGGGCAAATTCCCCTTATAGGGGTTCATTCAATAGGCGTTCCCCAAATGATAAATCAGGTATCAGGAGTGTTTCAATCTCCGGGAGTGGGAGGATACCGCCGTCTCCAGGCAGGATCAAAGAGAAGGAGGATGAAGAGATTTACAAGAAGGTTAAATTGAGTAGAGATAACCATAGGAAAGTGAAAATGACAACTAAGGTCTTGCTTGAGTGGGGTACTTTTCTGGTACTTCTGGCTTGTTTAGTGTCTAGCTTGACTATCGAGAAAGTAAAGAATGGTATGTTTTGGGGCTTGGAAATTTGGAAGTGGTGTGTGCTTGTGATGGTGATATTCTGTGGAATGTTGGTTACAAACTGGTTTATGCATTTCATAGTTTTTGTGATTGAGAGGAACTTCTTGCTGAGGAAAAAGGTTTTGTATTTTGTTCATGGCATGAAAAAGAGCGTTCAGGTTTCGATTTGGTTGGCTCTGGTTCTTCTCACATGGCTATTGGTGTTCAATCGGGGGGTCGAGCGATCAAAGACTTACACCAAGATTTTGAATTATGTCACATGGACTCTTGTTTCCATTCTTGTTGGGGCATTTTTGTGGTTGTTAAAAACTTTGTTCCTTAAGATTTTAGCATCTAGTTTCCATGTGAACACCTTTTTTGATAGAATCCAAGAATCAATCTTCCATCAGTATGTTCTTCAGACACTTTCAGGGCCGGCACTTATAGAGGAGGCTGTGAGAGATGGGAAATGTCCGAGCACTGGGCAAGTGAGTTTCAGGACGACAAAGAAGGCTAAGGCTGGGAAGGAGAAAGAGGTGATTGATATGGGAAAGCTTCAGAAGATGAAGCAAGAGAAGGTTTCAGCTTGGACCATGAAGGTATTGGTGGACGCAGTTTCAAGTTCAGGGCTGTCCACCATCTCCCATACATTGGATGAGATGGAAGCGGGAGGAGCTGAACAGACGGATGGGGAGATTACTAATGAGATGGAAGCAACCGCCGCTGCCTATCACATTTTTCGTAACGTCGCACAACCGGATTCCAAGTACATTGAGGTGGAGGACCTCATGAGGTTCATGATTAAAGAAGAGGTGGAACTTGTGTTTCCGCTGTTTGACACAGATGAGGCTGGACATATTGACCGAAAATCTCTAACGGATTGGGTGGTGAAGGTCTACAATGGTCGCAAAGCATTAGCTCATTCTTTAAACGACACCAAAACAGCTGTAAGGCAATTGAACAAACTTGTGACTGGCATCCTGGTTGTTATTACCATTGTAGTGTGGCTTTTATTGATGGAAATTGCAACCACGAAAGTGCTTGTTTTCCTCTCGTCACAGCTCGTAGTTGCAGCTTTTATGTTTGGAAACACTTGCAAGACTGTATTTGAAGCTATAATTTTTGTATTTGTAATGCACCCATTTGATGTTGGTGACCGCTGTGTTGTTGATGGTGTTCCGTTGATGGTTGAGGAGATGAACATCTTAAATACAGTCTTCTTGAAACTCAATATGGAGAAGGTATACTATCCGAATTCAGTTTTGTCCACAAAAGCCATCAGCAATTACTATAGAAGTCCAAACATGGGTGACATCGTCGAATTCCACATTGCTTTTTCGACAACAGTGGAGAAGATAGGCATGCTGAACGAGAAAATACAACATCATTTGGAGAGTAACTCGCAGCATTGGCATCCTAACCACAATCTGGTAGTGGCTGAAATCGAAAATCTCAATAAATTAAAGATGGCCCTGTATGTCACTCACACAATGAACTTTCAAGAGTTCGGAGAAAAGAATAAGCGGAGAACTGAACTAGTCATCGAATTGAAGAAAATGTTGGAAGAGCTCCAAATCAAGTACAATCTGCTGCCCCAAGAAGTTCGTCTCACTCAAGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

769

Amino Acids

86.72

Weight (kDa)

9.27

Isoelectric Point (pI)

44.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	202 - 349	4.2e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	602 - 659	5.7e-07	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	1336, 2113
AccI	GTMKAC	2 cut(s)	1581, 1929
AclWI	GGATC	2 cut(s)	133, 536
AcsI	RAATTY	5 cut(s)	251, 421, 711, 1939, 2006
AcuI	CTGAAG	2 cut(s)	1088, 1226
AfaI	GTAC	5 cut(s)	87, 631, 642, 1454, 2267
AflII	CTTAAG	1 cut(s)	1025
AhlI	ACTAGT	1 cut(s)	2221
AjnI	CCWGG	3 cut(s)	268, 520, 1665
AjuI	GAANNNNNNNTTGG	4 cut(s)	396, 428, 2228, 2260
AleI	CACNNNNGTG	1 cut(s)	1721
Alw21I	GWGCWC	3 cut(s)	239, 1166, 2256
Alw26I	GTCTC	2 cut(s)	522, 2297
AlwI	GGATC	2 cut(s)	133, 536
Ama87I	CYCGRG	1 cut(s)	142
AoxI	GGCC	3 cut(s)	6, 1118, 2154
ApeKI	GCWGC	5 cut(s)	116, 1409, 1757, 2089, 2275
ApoI	RAATTY	5 cut(s)	251, 421, 711, 1939, 2006
ArsI	GACNNNNNNTTYG	2 cut(s)	338, 370
AspS9I	GGNCC	5 cut(s)	226, 1118, 1275, 1468, 2155
AsuC2I	CCSGG	1 cut(s)	496
AsuHPI	GGTGA	9 cut(s)	89, 171, 237, 377, 751, 1234, 1585, 1850, 2006
AsuII	TTCGAA	1 cut(s)	97
AvaI	CYCGRG	1 cut(s)	142
AvaII	GGWCC	3 cut(s)	226, 1275, 1468
BaeI	ACNNNNGTAYC	2 cut(s)	500, 533
BanII	GRGCYC	3 cut(s)	179, 239, 2256
BbsI	GAAGAC	1 cut(s)	1894
Bbv12I	GWGCWC	3 cut(s)	239, 1166, 2256
BbvCI	CCTCAGC	1 cut(s)	815
BbvI	GCAGC	5 cut(s)	128, 1396, 1769, 2101, 2262
BceAI	ACGGC	1 cut(s)	499
BciT130I	CCWGG	3 cut(s)	270, 522, 1667
BciVI	GTATCC	1 cut(s)	501
BclI	TGATCA	1 cut(s)	385
BcnI	CCSGG	1 cut(s)	496
BcoDI	GTCTC	2 cut(s)	522, 2297
BcuI	ACTAGT	1 cut(s)	2221
BfaI	CTAG	4 cut(s)	312, 663, 1041, 2222
BfmI	CTRYAG	1 cut(s)	1975
BfrI	CTTAAG	1 cut(s)	1025
BfuI	GTATCC	1 cut(s)	501
BisI	GCNGC	6 cut(s)	117, 1407, 1410, 1758, 2090, 2276
BlsI	GCNGC	6 cut(s)	118, 1408, 1411, 1759, 2091, 2277
Bme1390I	CCNGG	4 cut(s)	270, 496, 522, 1667
Bme18I	GGWCC	3 cut(s)	226, 1275, 1468
BmeT110I	CYCGRG	1 cut(s)	142
BmgT120I	GGNCC	5 cut(s)	226, 1118, 1275, 1468, 2155
BmiI	GGNNCC	1 cut(s)	228
BmrFI	CCNGG	4 cut(s)	270, 496, 522, 1667
BmrI	ACTGGG	2 cut(s)	224, 1176
BmsI	GCATC	4 cut(s)	200, 1046, 1671, 2107
BmuI	ACTGGG	2 cut(s)	224, 1176
BpiI	GAAGAC	1 cut(s)	1894
BplI	GAGNNNNNCTC	2 cut(s)	2069, 2101
BpmI	CTGGAG	1 cut(s)	504
Bpu10I	CCTNAGC	2 cut(s)	815, 1200
Bpu14I	TTCGAA	1 cut(s)	97
BpuEI	CTTGAG	2 cut(s)	641, 2283
BpuMI	CCSGG	1 cut(s)	496
BsaBI	GATNNNNATC	1 cut(s)	1886
BsaWI	WCCGGW	1 cut(s)	1441
BsaXI	ACNNNNNCTCC	2 cut(s)	66, 96
Bse118I	RCCGGY	1 cut(s)	1120
Bse1I	ACTGG	6 cut(s)	93, 230, 264, 773, 1171, 1663
Bse3DI	GCAATG	2 cut(s)	304, 2013
Bse8I	GATNNNNATC	1 cut(s)	1886
BseBI	CCWGG	3 cut(s)	270, 522, 1667
BseGI	GGATG	5 cut(s)	550, 1345, 1378, 1662, 2098
BseJI	GATNNNNATC	1 cut(s)	1886
BseMI	GCAATG	2 cut(s)	304, 2013
BseMII	CTCAG	3 cut(s)	189, 387, 806
BseNI	ACTGG	6 cut(s)	93, 230, 264, 773, 1171, 1663
BseRI	GAGGAG	3 cut(s)	1148, 1371, 1892
BseXI	GCAGC	5 cut(s)	128, 1396, 1769, 2101, 2262
BseYI	CCCAGC	1 cut(s)	1205
BshFI	GGCC	3 cut(s)	8, 1120, 2156
BsiHKAI	GWGCWC	3 cut(s)	239, 1166, 2256
BsiHKCI	CYCGRG	1 cut(s)	142
BsiSI	CCGG	3 cut(s)	495, 1121, 1442
BslFI	GGGAC	1 cut(s)	212
BsmAI	GTCTC	2 cut(s)	522, 2297
BsmBI	CGTCTC	2 cut(s)	522, 2297
BsmFI	GGGAC	1 cut(s)	212
BsnI	GGCC	3 cut(s)	8, 1120, 2156
BsoBI	CYCGRG	1 cut(s)	142
Bsp119I	TTCGAA	1 cut(s)	97
Bsp1286I	GDGCHC	4 cut(s)	179, 239, 1166, 2256
Bsp143I	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
BspANI	GGCC	3 cut(s)	8, 1120, 2156
BspCNI	CTCAG	3 cut(s)	190, 386, 807
BspHI	TCATGA	2 cut(s)	1473, 1482
BspLI	GGNNCC	1 cut(s)	228
BspPI	GGATC	2 cut(s)	133, 536
BspQI	GCTCTTC	1 cut(s)	2244
BspT104I	TTCGAA	1 cut(s)	97
BspTI	CTTAAG	1 cut(s)	1025
BsrDI	GCAATG	2 cut(s)	304, 2013
BsrFI	RCCGGY	1 cut(s)	1120
BsrI	ACTGG	6 cut(s)	93, 230, 264, 773, 1171, 1663
BssAI	RCCGGY	1 cut(s)	1120
BssMI	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
BssNAI	GTATAC	1 cut(s)	1930
Bst1107I	GTATAC	1 cut(s)	1930
Bst2UI	CCWGG	3 cut(s)	270, 522, 1667
Bst4CI	ACNGT	3 cut(s)	1789, 1900, 2032
Bst6I	CTCTTC	4 cut(s)	99, 543, 1488, 2244
BstAFI	CTTAAG	1 cut(s)	1025
BstBI	TTCGAA	1 cut(s)	97
BstC8I	GCNNGC	2 cut(s)	1122, 2048
BstDEI	CTNAG	6 cut(s)	198, 219, 373, 609, 815, 1200
BstEII	GGTNACC	1 cut(s)	1838
BstF5I	GGATG	5 cut(s)	550, 1345, 1378, 1662, 2098
BstKTI	GATC	6 cut(s)	111, 128, 388, 409, 531, 932
BstMAI	GTCTC	2 cut(s)	522, 2297
BstMBI	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
BstMWI	GCNNNNNNNGC	4 cut(s)	174, 1358, 1406, 2095
BstNI	CCWGG	3 cut(s)	270, 522, 1667
BstNSI	RCATGY	2 cut(s)	52, 2050
BstPI	GGTNACC	1 cut(s)	1838
BstSCI	CCNGG	4 cut(s)	268, 494, 520, 1665
BstSFI	CTRYAG	1 cut(s)	1975
BstV1I	GCAGC	5 cut(s)	128, 1396, 1769, 2101, 2262
BstV2I	GAAGAC	1 cut(s)	1894
BstX2I	RGATCY	1 cut(s)	125
BstYI	RGATCY	1 cut(s)	125
BstZ17I	GTATAC	1 cut(s)	1930
BsuI	GTATCC	1 cut(s)	501
BsuRI	GGCC	3 cut(s)	8, 1120, 2156
BtsCI	GGATG	5 cut(s)	550, 1345, 1378, 1662, 2098
BtsI	GCAGTG	1 cut(s)	214
BtsIMutI	CAGTG	4 cut(s)	214, 237, 1164, 2037
Cac8I	GCNNGC	2 cut(s)	1122, 2048
CciI	TCATGA	2 cut(s)	1473, 1482
Cfr10I	RCCGGY	1 cut(s)	1120
Cfr13I	GGNCC	5 cut(s)	226, 1118, 1275, 1468, 2155
CseI	GACGC	1 cut(s)	1304
Csp6I	GTAC	5 cut(s)	86, 630, 641, 1453, 2266
CviQI	GTAC	5 cut(s)	86, 630, 641, 1453, 2266
DdeI	CTNAG	6 cut(s)	198, 219, 373, 609, 815, 1200
DpnI	GATC	6 cut(s)	110, 127, 387, 408, 530, 931
DpnII	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
DraI	TTTAAA	1 cut(s)	1614
Eam1104I	CTCTTC	4 cut(s)	99, 543, 1488, 2244
EarI	CTCTTC	4 cut(s)	99, 543, 1488, 2244
Ecl136II	GAGCTC	2 cut(s)	237, 2254
Eco24I	GRGCYC	3 cut(s)	179, 239, 2256
Eco47I	GGWCC	3 cut(s)	226, 1275, 1468
Eco53kI	GAGCTC	2 cut(s)	237, 2254
Eco57I	CTGAAG	2 cut(s)	1088, 1226
Eco88I	CYCGRG	1 cut(s)	142
Eco91I	GGTNACC	1 cut(s)	1838
EcoICRI	GAGCTC	2 cut(s)	237, 2254
EcoO109I	RGGNCCY	1 cut(s)	1468
EcoO65I	GGTNACC	1 cut(s)	1838
EcoRI	GAATTC	2 cut(s)	1939, 2006
EcoRII	CCWGG	3 cut(s)	268, 520, 1665
EcoT22I	ATGCAT	1 cut(s)	780
EcoT38I	GRGCYC	3 cut(s)	179, 239, 2256
Esp3I	CGTCTC	2 cut(s)	522, 2297
FaqI	GGGAC	1 cut(s)	212
FauI	CCCGC	4 cut(s)	29, 190, 302, 1345
FbaI	TGATCA	1 cut(s)	385
FblI	GTMKAC	2 cut(s)	1581, 1929
Fnu4HI	GCNGC	6 cut(s)	117, 1407, 1410, 1758, 2090, 2276
FokI	GGATG	5 cut(s)	557, 1352, 1385, 1649, 2085
FriOI	GRGCYC	3 cut(s)	179, 239, 2256
Fsp4HI	GCNGC	6 cut(s)	117, 1407, 1410, 1758, 2090, 2276
FspBI	CTAG	4 cut(s)	312, 663, 1041, 2222
GluI	GCNGC	6 cut(s)	117, 1407, 1410, 1758, 2090, 2276
GsaI	CCCAGC	1 cut(s)	1209
GsuI	CTGGAG	1 cut(s)	504
HaeIII	GGCC	3 cut(s)	8, 1120, 2156
HapII	CCGG	3 cut(s)	495, 1121, 1442
HgaI	GACGC	1 cut(s)	1304
HincII	GTYRAC	1 cut(s)	336
HindII	GTYRAC	1 cut(s)	336
HindIII	AAGCTT	4 cut(s)	23, 59, 1238, 2301
HinfI	GANTC	7 cut(s)	200, 286, 304, 970, 1071, 1079, 1445
HpaII	CCGG	3 cut(s)	495, 1121, 1442
HphI	GGTGA	9 cut(s)	89, 171, 237, 377, 751, 1234, 1585, 1850, 2006
Hpy166II	GTNNAC	8 cut(s)	336, 368, 1054, 1294, 1320, 1582, 1930, 1953
Hpy188I	TCNGA	6 cut(s)	55, 1107, 1161, 1245, 1938, 2195
Hpy8I	GTNNAC	8 cut(s)	336, 368, 1054, 1294, 1320, 1582, 1930, 1953
Hpy99I	CGWCG	2 cut(s)	1436, 2006
HpyAV	CCTTC	9 cut(s)	252, 532, 556, 1189, 1204, 1255, 1276, 1570, 1918
HpyCH4III	ACNGT	3 cut(s)	1789, 1900, 2032
HpyCH4IV	ACGT	1 cut(s)	1431
HpyCH4V	TGCA	6 cut(s)	116, 778, 1712, 1757, 1782, 1822
HpyF10VI	GCNNNNNNNGC	4 cut(s)	174, 1358, 1406, 2095
HpyF3I	CTNAG	6 cut(s)	198, 219, 373, 609, 815, 1200
HpySE526I	ACGT	1 cut(s)	1431
KroI	GCCGGC	1 cut(s)	1120
KroNI	GCCGGC	1 cut(s)	1122
Ksp22I	TGATCA	1 cut(s)	385
Kzo9I	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
LguI	GCTCTTC	1 cut(s)	2244
LmnI	GCTCC	4 cut(s)	160, 242, 1358, 2259
Lsp1109I	GCAGC	5 cut(s)	128, 1396, 1769, 2101, 2262
LweI	GCATC	4 cut(s)	200, 1046, 1671, 2107
MaeI	CTAG	4 cut(s)	312, 663, 1041, 2222
MaeII	ACGT	1 cut(s)	1431
MalI	GATC	6 cut(s)	110, 127, 387, 408, 530, 931
MboI	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
MfeI	CAATTG	1 cut(s)	1640
MflI	RGATCY	1 cut(s)	125
MhlI	GDGCHC	4 cut(s)	179, 239, 1166, 2256
MlyI	GAGTC	3 cut(s)	209, 313, 964
MmeI	TCCRAC	2 cut(s)	33, 2226
Mph1103I	ATGCAT	1 cut(s)	780
MroNI	GCCGGC	1 cut(s)	1120
MseI	TTAA	8 cut(s)	567, 1010, 1026, 1488, 1613, 1892, 2147, 2305
MslI	CAYNNNNRTG	5 cut(s)	1000, 1095, 1460, 1685, 1721
MspA1I	CMGCKG	4 cut(s)	1409, 1516, 1631, 1845
MspCI	CTTAAG	1 cut(s)	1025
MspI	CCGG	3 cut(s)	495, 1121, 1442
MspR9I	CCNGG	4 cut(s)	270, 496, 522, 1667
MunI	CAATTG	1 cut(s)	1640
MvaI	CCWGG	3 cut(s)	270, 522, 1667
MwoI	GCNNNNNNNGC	4 cut(s)	174, 1358, 1406, 2095
NaeI	GCCGGC	1 cut(s)	1122
NciI	CCSGG	1 cut(s)	496
NdeII	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
NgoMIV	GCCGGC	1 cut(s)	1120
NlaIV	GGNNCC	1 cut(s)	228
NmuCI	GTSAC	7 cut(s)	321, 961, 1654, 1740, 1838, 1994, 2164
NsiI	ATGCAT	1 cut(s)	780
NspI	RCATGY	2 cut(s)	52, 2050
NspV	TTCGAA	1 cut(s)	97
OliI	CACNNNNGTG	1 cut(s)	1721
PaeI	GCATGC	1 cut(s)	2050
PagI	TCATGA	2 cut(s)	1473, 1482
PciSI	GCTCTTC	1 cut(s)	2244
PdiI	GCCGGC	1 cut(s)	1122
PfeI	GAWTC	4 cut(s)	286, 1071, 1079, 1445
PflFI	GACNNNGTC	1 cut(s)	2000
PflMI	CCANNNNNTGG	2 cut(s)	1336, 2113
PfoI	TCCNGGA	1 cut(s)	494
PkrI	GCNGC	6 cut(s)	118, 1408, 1411, 1759, 2091, 2277
PleI	GAGTC	3 cut(s)	208, 312, 964
PpsI	GAGTC	3 cut(s)	208, 312, 964
PpuMI	RGGWCCY	1 cut(s)	1468
Psp124BI	GAGCTC	2 cut(s)	239, 2256
Psp5II	RGGWCCY	1 cut(s)	1468
Psp6I	CCWGG	3 cut(s)	268, 520, 1665
PspEI	GGTNACC	1 cut(s)	1838
PspFI	CCCAGC	1 cut(s)	1205
PspGI	CCWGG	3 cut(s)	268, 520, 1665
PspN4I	GGNNCC	1 cut(s)	228
PspPI	GGNCC	5 cut(s)	226, 1118, 1275, 1468, 2155
PspPPI	RGGWCCY	1 cut(s)	1468
PsrI	GAACNNNNNNTAC	2 cut(s)	78, 110
PsuI	RGATCY	1 cut(s)	125
PsyI	GACNNNGTC	1 cut(s)	2000
PvuII	CAGCTG	1 cut(s)	1631
RsaI	GTAC	5 cut(s)	87, 631, 642, 1454, 2267
RsaNI	GTAC	5 cut(s)	86, 630, 641, 1453, 2266
RseI	CAYNNNNRTG	5 cut(s)	1000, 1095, 1460, 1685, 1721
SacI	GAGCTC	2 cut(s)	239, 2256
SapI	GCTCTTC	1 cut(s)	2244
SaqAI	TTAA	8 cut(s)	567, 1010, 1026, 1488, 1613, 1892, 2147, 2305
SatI	GCNGC	6 cut(s)	117, 1407, 1410, 1758, 2090, 2276
Sau3AI	GATC	6 cut(s)	108, 125, 385, 406, 528, 929
Sau96I	GGNCC	5 cut(s)	226, 1118, 1275, 1468, 2155
SchI	GAGTC	3 cut(s)	209, 313, 964
ScrFI	CCNGG	4 cut(s)	270, 496, 522, 1667
SduI	GDGCHC	4 cut(s)	179, 239, 1166, 2256
SfaNI	GCATC	4 cut(s)	200, 1046, 1671, 2107
SfcI	CTRYAG	1 cut(s)	1975
SfuI	TTCGAA	1 cut(s)	97
SinI	GGWCC	3 cut(s)	226, 1275, 1468
SmiMI	CAYNNNNRTG	5 cut(s)	1000, 1095, 1460, 1685, 1721
SmlI	CTYRAG	3 cut(s)	620, 1025, 2298
SmoI	CTYRAG	3 cut(s)	620, 1025, 2298
SpeI	ACTAGT	1 cut(s)	2221
SphI	GCATGC	1 cut(s)	2050
SspMI	CTAG	4 cut(s)	312, 663, 1041, 2222
SstI	GAGCTC	2 cut(s)	239, 2256
StyD4I	CCNGG	4 cut(s)	268, 494, 520, 1665
TaaI	ACNGT	3 cut(s)	1789, 1900, 2032
TaiI	ACGT	1 cut(s)	1434
TaqII	GACCGA	2 cut(s)	214, 1563
TatI	WGTACW	3 cut(s)	85, 1452, 2265
TauI	GCSGC	1 cut(s)	1409
TfiI	GAWTC	4 cut(s)	286, 1071, 1079, 1445
Tru1I	TTAA	8 cut(s)	567, 1010, 1026, 1488, 1613, 1892, 2147, 2305
Tru9I	TTAA	8 cut(s)	567, 1010, 1026, 1488, 1613, 1892, 2147, 2305
TscAI	CASTG	4 cut(s)	214, 237, 1171, 2037
TseFI	GTSAC	7 cut(s)	321, 961, 1654, 1740, 1838, 1994, 2164
TseI	GCWGC	5 cut(s)	116, 1409, 1757, 2089, 2275
Tsp45I	GTSAC	7 cut(s)	321, 961, 1654, 1740, 1838, 1994, 2164
TspGWI	ACGGA	3 cut(s)	1385, 1577, 1854
TspRI	CASTG	4 cut(s)	214, 237, 1171, 2037
Tth111I	GACNNNGTC	1 cut(s)	2000
Van91I	CCANNNNNTGG	2 cut(s)	1336, 2113
Vha464I	CTTAAG	1 cut(s)	1025
VpaK11BI	GGWCC	3 cut(s)	226, 1275, 1468
XapI	RAATTY	5 cut(s)	251, 421, 711, 1939, 2006
XceI	RCATGY	2 cut(s)	52, 2050
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1285
XmiI	GTMKAC	2 cut(s)	1581, 1929
XspI	CTAG	4 cut(s)	312, 663, 1041, 2222
Zsp2I	ATGCAT	1 cut(s)	780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.