Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Forward (+)

68443545 .. 68446612

Gene Structure 3068 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ28022

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGCAAGCTTTCCTTCGCTTATTAAGTGACAGGTTCTTGATGGAAGCCGCAGAGAAAGGCTTAAAAGGAAGCAATGTAAGCATGTCGAAAAATCGATCTGGGAATGATGGAGGTGATGAAGTTGTAGTGGTGGTTCAAAGCGAAGAGATGGAAGATGAAGCAAAAGGGTCTTGTTCGAAAGGCTTAGAATCTTCAGCTCCCAATCAAATTAGCAAAGTGGAATTGCAGGTTGGCTCTCATTCCCCTGAGATTACAGGATCCAGCAACGCCCCCATCCCCAATGAGACCCTCACTAGGAGAAAGTCATCTATTAGTCTTGGTCAACAGTCGATGCGCATTGATTCTGATATTCTTGAAGAGGACACTAATTTAGAGCAGGATGGTTCCCCCGCAAATAAATCAGCATTCAAAGAGGAGGAGGAGGAGGAGGAGGAGATTTACGAGAAGGTTAACTCTAGCAGGAAAGTGAACAAGAAAATGCTGATGGAGTATATTGTTTTCGCGTGCAGTTTGGGTGTTATAGTAGCTAGCTTTACTGTCGAGAAACTAGAGAATCTTAAGGTGTGGGGATTGGAGATGTGGAAATTGAACTTTCTGTTTACGAGGAAGAAGGTGGTGTTGTATTTGGTTTATGGAATGAAAAAGAGTGTTCAGGTTTTCCTGTGGTTCGGTATGGTGCTTCTCACATGGCTATTCTTTTTCAAATTCAATCGTGGCAATGAGAGATCAAAGACTTCCACCAAGATTTTGGATCATATTACGTGGACTCTTGTTTCGCTGCTTATTGGTGCCTTTTTGTGGATGTTATCAACTTTGTTGTTTAAGACCCTAGCATCTAAATTCATGGCCGTCGCCTATTTTGATAGGATTCAAGAATCTTTGTTCCATCAGTACGTTATTGAGACACTTTCGGGGCCTCCGTTAGTAGAGGAGGATGCCAAGATTAGGAGATCACTCAGCACAGGGTTTGTGAGTCTCAGGTCGATAAACAATGTGGAAAAGAAGGTCACTGGTAAGGTGAATCCTGCAAGGAAGGTTTCAGTTGAGACCATGGATACGTTGGTGGGTGCAGTTTCTACTAGAGATACAGAGATTACAAGTGAGTGGGAAGCCAAAGCTGCTGCCTTCAAAATATTTCGAAACGTTGCTCAGCATGGTAACAAGTACATCGAGGAGGAGGACCTCATGAGGTTCATGACTCGGGCAGAGGTGGGTAATGTATTTCCACTGTTTGACGGAGCAATGGAAAACGGCAAAATTAAAAGAAAAGATCTACGTAGCTGGATGGTAAATGTCTACCGAGAACGCAACGCATTAGCTTGTTCTATGACTGACACCAAAACAGTTCTAAAGCAATTTGAGAAATTTTTTACTAGCTTGGTGATTGTTATTACCATCGTAGTGTGGCTACTGTTGGTGGGAATTGCAAGTACAAACCTAGTTGTTTTCCTCTCAACACAGTTCTTATTGGCAGCTTTTATTTTTGGAAACACTTGCAAGAATGTATTTGAAGCTATCGTGTTCATGTTTGTGACACATCCATTTGATGTTGGTGATCGCATTGTTGTTGATGATGTTCTGTTGGTCGTTGAAGAGATGAACGTCTTAACTACCACCTTCTTGAAACCAAATAATGAAAAGGTGTACTATCCAAATTCAGTTTTGTGCACCAAAATCATCAGCAATTATTACAGAAGTCCAGAAATGGGAGATACAGTGGAATTCTCAATTATTTTGACACCAGTGGAGAAGATAGACATGTTGAGAAAGAGAATAAAGGAGTACTTGGAGAGCAAACCGCAGCATTGGCGTCCAGGCCACAGTGTGGTGGTGATTGATATCGAAGATGTGAATAAGTTAAAGATGGCACTCAATATTTGTCACACTATGAACTTTCAAGAAATTGGTGAAAGGAATAAACGGCGAACTGAGCTAGTCATCGAATTGATGAGAACTTTGGAAGACCTAAACATCACATACAGTCTGATGCCCCAAGAAGTTCACCTCACTCAAAATAAAGCAGATGCAACCGTTTCCTTCAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

682

Amino Acids

77.81

Weight (kDa)

6.58

Isoelectric Point (pI)

42.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	500 - 564	1.4e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	335
Acc36I	ACCTGC	1 cut(s)	217
AccB1I	GGYRCC	1 cut(s)	788
AccB7I	CCANNNNNTGG	1 cut(s)	1825
AccI	GTMKAC	1 cut(s)	1296
AccII	CGCG	1 cut(s)	503
AciI	CCGC	3 cut(s)	48, 390, 1799
AclI	AACGTT	1 cut(s)	1143
AclWI	GGATC	3 cut(s)	252, 265, 759
AcoI	YGGCCR	1 cut(s)	846
AcsI	RAATTY	5 cut(s)	704, 839, 1364, 1654, 1721
AcuI	CTGAAG	1 cut(s)	177
AcyI	GRCGYC	1 cut(s)	1810
AdeI	CACNNNGTG	1 cut(s)	1825
AfaI	GTAC	5 cut(s)	893, 1166, 1432, 1646, 1784
AfiI	CCNNNNNNNGG	5 cut(s)	294, 798, 945, 1706, 1825
AflII	CTTAAG	1 cut(s)	557
AflIII	ACRYGT	1 cut(s)	1758
AjnI	CCWGG	1 cut(s)	1813
AjuI	GAANNNNNNNTTGG	2 cut(s)	213, 245
Alw21I	GWGCWC	1 cut(s)	1670
Alw26I	GTCTC	4 cut(s)	278, 896, 980, 1040
Alw44I	GTGCAC	1 cut(s)	1666
AlwI	GGATC	3 cut(s)	252, 265, 759
Ama87I	CYCGRG	1 cut(s)	1200
AoxI	GGCC	3 cut(s)	846, 914, 1816
ApaLI	GTGCAC	1 cut(s)	1666
ApeKI	GCWGC	5 cut(s)	778, 1118, 1121, 1472, 1801
ApoI	RAATTY	5 cut(s)	704, 839, 1364, 1654, 1721
ArsI	GACNNNNNNTTYG	2 cut(s)	757, 789
AspLEI	GCGC	1 cut(s)	336
AspS9I	GGNCC	2 cut(s)	914, 1180
AsuHPI	GGTGA	7 cut(s)	125, 1030, 1393, 1565, 1843, 1919, 1994
AsuII	TTCGAA	2 cut(s)	176, 1138
AsuNHI	GCTAGC	1 cut(s)	527
AvaI	CYCGRG	1 cut(s)	1200
AvaII	GGWCC	1 cut(s)	1180
BaeGI	GKGCMC	1 cut(s)	1670
BaeI	ACNNNNGTAYC	2 cut(s)	1047, 1080
BamHI	GGATCC	1 cut(s)	257
BanI	GGYRCC	1 cut(s)	788
BbsI	GAAGAC	1 cut(s)	1968
Bbv12I	GWGCWC	1 cut(s)	1670
BbvI	GCAGC	5 cut(s)	765, 1105, 1108, 1484, 1813
BceAI	ACGGC	3 cut(s)	833, 1267, 1937
BciT130I	CCWGG	1 cut(s)	1815
BciVI	GTATCC	1 cut(s)	1048
BcoDI	GTCTC	4 cut(s)	278, 896, 980, 1040
BfaI	CTAG	9 cut(s)	294, 456, 528, 548, 830, 1080, 1374, 1439, 1934
BfrI	CTTAAG	1 cut(s)	557
BfuAI	ACCTGC	1 cut(s)	217
BfuI	GTATCC	1 cut(s)	1048
BglII	AGATCT	1 cut(s)	1270
BisI	GCNGC	6 cut(s)	48, 779, 1119, 1122, 1473, 1802
BlpI	GCTNAGC	1 cut(s)	1149
BlsI	GCNGC	6 cut(s)	49, 780, 1120, 1123, 1474, 1803
BmcAI	AGTACT	1 cut(s)	1784
Bme1390I	CCNGG	1 cut(s)	1815
Bme18I	GGWCC	1 cut(s)	1180
BmeT110I	CYCGRG	1 cut(s)	1200
BmgT120I	GGNCC	2 cut(s)	914, 1180
BmiI	GGNNCC	4 cut(s)	259, 385, 790, 915
BmrFI	CCNGG	1 cut(s)	1815
BmsI	GCATC	5 cut(s)	321, 842, 925, 1977, 2014
BmtI	GCTAGC	1 cut(s)	531
BpiI	GAAGAC	1 cut(s)	1968
Bpu1102I	GCTNAGC	1 cut(s)	1149
Bpu14I	TTCGAA	2 cut(s)	176, 1138
Bsa29I	ATCGAT	1 cut(s)	94
BsaAI	YACGTR	2 cut(s)	762, 1277
BsaBI	GATNNNNATC	1 cut(s)	1838
BsaHI	GRCGYC	1 cut(s)	1810
BsaI	GGTCTC	2 cut(s)	278, 1040
BsaJI	CCNNGG	1 cut(s)	1050
BsaXI	ACNNNNNCTCC	2 cut(s)	1701, 1731
Bsc4I	CCNNNNNNNGG	5 cut(s)	294, 798, 945, 1706, 1825
Bse1I	ACTGG	2 cut(s)	1015, 1742
Bse3DI	GCAATG	3 cut(s)	79, 724, 1248
Bse8I	GATNNNNATC	1 cut(s)	1838
BseBI	CCWGG	1 cut(s)	1815
BseCI	ATCGAT	1 cut(s)	94
BseDI	CCNNGG	1 cut(s)	1050
BseGI	GGATG	6 cut(s)	273, 385, 807, 940, 1290, 1537
BseJI	GATNNNNATC	1 cut(s)	1838
BseLI	CCNNNNNNNGG	5 cut(s)	294, 798, 945, 1706, 1825
BseMI	GCAATG	3 cut(s)	79, 724, 1248
BseMII	CTCAG	5 cut(s)	237, 970, 991, 1163, 1920
BseNI	ACTGG	2 cut(s)	1015, 1742
BseSI	GKGCMC	1 cut(s)	1670
BseXI	GCAGC	5 cut(s)	765, 1105, 1108, 1484, 1813
BsgI	GTGCAG	2 cut(s)	526, 1089
Bsh1236I	CGCG	1 cut(s)	503
BshFI	GGCC	3 cut(s)	848, 916, 1818
BshNI	GGYRCC	1 cut(s)	788
BshVI	ATCGAT	1 cut(s)	94
BsiHKAI	GWGCWC	1 cut(s)	1670
BsiHKCI	CYCGRG	1 cut(s)	1200
BslI	CCNNNNNNNGG	5 cut(s)	294, 798, 945, 1706, 1825
BsmAI	GTCTC	4 cut(s)	278, 896, 980, 1040
BsmI	GAATGC	1 cut(s)	404
BsnI	GGCC	3 cut(s)	848, 916, 1818
Bso31I	GGTCTC	2 cut(s)	278, 1040
BsoBI	CYCGRG	1 cut(s)	1200
Bsp119I	TTCGAA	2 cut(s)	176, 1138
Bsp1286I	GDGCHC	1 cut(s)	1670
Bsp143I	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
Bsp1720I	GCTNAGC	1 cut(s)	1149
Bsp19I	CCATGG	1 cut(s)	1050
BspACI	CCGC	3 cut(s)	48, 390, 1799
BspANI	GGCC	3 cut(s)	848, 916, 1818
BspCNI	CTCAG	5 cut(s)	238, 969, 990, 1162, 1921
BspDI	ATCGAT	1 cut(s)	94
BspFNI	CGCG	1 cut(s)	503
BspHI	TCATGA	2 cut(s)	1185, 1194
BspLI	GGNNCC	4 cut(s)	259, 385, 790, 915
BspMI	ACCTGC	1 cut(s)	217
BspOI	GCTAGC	1 cut(s)	531
BspPI	GGATC	3 cut(s)	252, 265, 759
BspT104I	TTCGAA	2 cut(s)	176, 1138
BspT107I	GGYRCC	1 cut(s)	788
BspTI	CTTAAG	1 cut(s)	557
BspTNI	GGTCTC	2 cut(s)	278, 1040
BsrDI	GCAATG	3 cut(s)	79, 724, 1248
BsrI	ACTGG	2 cut(s)	1015, 1742
BssECI	CCNNGG	1 cut(s)	1050
BssMI	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
BssNI	GRCGYC	1 cut(s)	1810
BssT1I	CCWWGG	1 cut(s)	1050
Bst2UI	CCWGG	1 cut(s)	1815
Bst6I	CTCTTC	3 cut(s)	138, 351, 1587
BstACI	GRCGYC	1 cut(s)	1810
BstAFI	CTTAAG	1 cut(s)	557
BstBAI	YACGTR	2 cut(s)	762, 1277
BstBI	TTCGAA	2 cut(s)	176, 1138
BstC8I	GCNNGC	3 cut(s)	6, 505, 529
BstDEI	CTNAG	6 cut(s)	184, 246, 956, 977, 1149, 1929
BstDSI	CCRYGG	1 cut(s)	1050
BstF5I	GGATG	6 cut(s)	273, 385, 807, 940, 1290, 1537
BstFNI	CGCG	1 cut(s)	503
BstHHI	GCGC	1 cut(s)	336
BstKTI	GATC	7 cut(s)	98, 260, 728, 754, 953, 1273, 1558
BstMAI	GTCTC	4 cut(s)	278, 896, 980, 1040
BstMBI	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
BstMWI	GCNNNNNNNGC	4 cut(s)	78, 1118, 1807, 1930
BstNI	CCWGG	1 cut(s)	1815
BstNSI	RCATGY	2 cut(s)	85, 1762
BstSCI	CCNGG	1 cut(s)	1813
BstSLI	GKGCMC	1 cut(s)	1670
BstSNI	TACGTA	1 cut(s)	1277
BstUI	CGCG	1 cut(s)	503
BstV1I	GCAGC	5 cut(s)	765, 1105, 1108, 1484, 1813
BstV2I	GAAGAC	1 cut(s)	1968
BstX2I	RGATCY	2 cut(s)	257, 1270
BstXI	CCANNNNNNTGG	1 cut(s)	748
BstYI	RGATCY	2 cut(s)	257, 1270
Bsu15I	ATCGAT	1 cut(s)	94
BsuI	GTATCC	1 cut(s)	1048
BsuRI	GGCC	3 cut(s)	848, 916, 1818
BsuTUI	ATCGAT	1 cut(s)	94
BtgI	CCRYGG	1 cut(s)	1050
BtsCI	GGATG	6 cut(s)	273, 385, 807, 940, 1290, 1537
BtsIMutI	CAGTG	5 cut(s)	1008, 1226, 1722, 1749, 1828
BveI	ACCTGC	1 cut(s)	217
Cac8I	GCNNGC	3 cut(s)	6, 505, 529
CciI	TCATGA	2 cut(s)	1185, 1194
CfoI	GCGC	1 cut(s)	336
Cfr13I	GGNCC	2 cut(s)	914, 1180
ClaI	ATCGAT	1 cut(s)	94
CseI	GACGC	1 cut(s)	1799
Csp6I	GTAC	5 cut(s)	892, 1165, 1431, 1645, 1783
CviAII	CATG	9 cut(s)	82, 687, 844, 1051, 1154, 1186, 1195, 1525, 1759
CviQI	GTAC	5 cut(s)	892, 1165, 1431, 1645, 1783
DdeI	CTNAG	6 cut(s)	184, 246, 956, 977, 1149, 1929
DpnI	GATC	7 cut(s)	97, 259, 727, 753, 952, 1272, 1557
DpnII	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
DraIII	CACNNNGTG	1 cut(s)	1825
EaeI	YGGCCR	1 cut(s)	846
Eam1104I	CTCTTC	3 cut(s)	138, 351, 1587
EarI	CTCTTC	3 cut(s)	138, 351, 1587
Eco105I	TACGTA	1 cut(s)	1277
Eco130I	CCWWGG	1 cut(s)	1050
Eco31I	GGTCTC	2 cut(s)	278, 1040
Eco32I	GATATC	1 cut(s)	1840
Eco47I	GGWCC	1 cut(s)	1180
Eco57I	CTGAAG	1 cut(s)	177
Eco88I	CYCGRG	1 cut(s)	1200
EcoO109I	RGGNCCY	2 cut(s)	914, 1180
EcoRI	GAATTC	1 cut(s)	1721
EcoRII	CCWGG	1 cut(s)	1813
EcoRV	GATATC	1 cut(s)	1840
EcoT14I	CCWWGG	1 cut(s)	1050
ErhI	CCWWGG	1 cut(s)	1050
FaeI	CATG	9 cut(s)	85, 690, 847, 1054, 1157, 1189, 1198, 1528, 1762
FalI	AAGNNNNNCTT	3 cut(s)	29, 1769, 1801
FatI	CATG	9 cut(s)	81, 686, 843, 1050, 1153, 1185, 1194, 1524, 1758
FauI	CCCGC	1 cut(s)	397
FblI	GTMKAC	1 cut(s)	1296
Fnu4HI	GCNGC	6 cut(s)	48, 779, 1119, 1122, 1473, 1802
FokI	GGATG	6 cut(s)	260, 392, 814, 947, 1297, 1524
Fsp4HI	GCNGC	6 cut(s)	48, 779, 1119, 1122, 1473, 1802
FspAI	RTGCGCAY	1 cut(s)	335
FspBI	CTAG	9 cut(s)	294, 456, 528, 548, 830, 1080, 1374, 1439, 1934
FspI	TGCGCA	1 cut(s)	335
GlaI	GCGC	1 cut(s)	335
GluI	GCNGC	6 cut(s)	48, 779, 1119, 1122, 1473, 1802
HaeIII	GGCC	3 cut(s)	848, 916, 1818
HgaI	GACGC	1 cut(s)	1799
HhaI	GCGC	1 cut(s)	336
Hin1I	GRCGYC	1 cut(s)	1810
Hin1II	CATG	9 cut(s)	85, 690, 847, 1054, 1157, 1189, 1198, 1528, 1762
Hin6I	GCGC	1 cut(s)	334
HinP1I	GCGC	1 cut(s)	334
HincII	GTYRAC	2 cut(s)	323, 451
HindII	GTYRAC	2 cut(s)	323, 451
HindIII	AAGCTT	1 cut(s)	6
HinfI	GANTC	9 cut(s)	188, 341, 553, 766, 868, 875, 973, 1021, 1198
HpaI	GTTAAC	1 cut(s)	451
HphI	GGTGA	7 cut(s)	125, 1030, 1393, 1565, 1843, 1919, 1994
Hpy166II	GTNNAC	9 cut(s)	323, 451, 469, 600, 765, 1297, 1645, 1668, 2002
Hpy188I	TCNGA	2 cut(s)	346, 1986
Hpy8I	GTNNAC	9 cut(s)	323, 451, 469, 600, 765, 1297, 1645, 1668, 2002
Hpy99I	CGWCG	1 cut(s)	854
HpyAV	CCTTC	8 cut(s)	23, 439, 604, 997, 1027, 1135, 1627, 2047
HpyCH4IV	ACGT	6 cut(s)	761, 894, 1058, 1143, 1276, 1602
HpyCH4V	TGCA	9 cut(s)	4, 226, 507, 1028, 1070, 1427, 1497, 1668, 2027
HpyF10VI	GCNNNNNNNGC	4 cut(s)	78, 1118, 1807, 1930
HpyF3I	CTNAG	6 cut(s)	184, 246, 956, 977, 1149, 1929
HpySE526I	ACGT	6 cut(s)	761, 894, 1058, 1143, 1276, 1602
Hsp92I	GRCGYC	1 cut(s)	1810
Hsp92II	CATG	9 cut(s)	85, 690, 847, 1054, 1157, 1189, 1198, 1528, 1762
HspAI	GCGC	1 cut(s)	334
KspAI	GTTAAC	1 cut(s)	451
Kzo9I	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
LmnI	GCTCC	2 cut(s)	202, 1238
Lsp1109I	GCAGC	5 cut(s)	765, 1105, 1108, 1484, 1813
LweI	GCATC	5 cut(s)	321, 842, 925, 1977, 2014
MaeI	CTAG	9 cut(s)	294, 456, 528, 548, 830, 1080, 1374, 1439, 1934
MaeII	ACGT	6 cut(s)	761, 894, 1058, 1143, 1276, 1602
MaeIII	GTNAC	5 cut(s)	26, 1006, 1157, 1531, 1880
MalI	GATC	7 cut(s)	97, 259, 727, 753, 952, 1272, 1557
MboI	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
MboII	GAAGA	9 cut(s)	155, 164, 183, 368, 619, 1604, 1762, 1856, 1973
MflI	RGATCY	2 cut(s)	257, 1270
MhlI	GDGCHC	1 cut(s)	1670
MlyI	GAGTC	3 cut(s)	760, 982, 1192
MseI	TTAA	8 cut(s)	23, 62, 450, 558, 822, 1260, 1607, 1859
MslI	CAYNNNNRTG	2 cut(s)	1400, 1529
MspCI	CTTAAG	1 cut(s)	557
MspR9I	CCNGG	1 cut(s)	1815
Mva1269I	GAATGC	1 cut(s)	404
MvaI	CCWGG	1 cut(s)	1815
MvnI	CGCG	1 cut(s)	503
MwoI	GCNNNNNNNGC	4 cut(s)	78, 1118, 1807, 1930
NcoI	CCATGG	1 cut(s)	1050
NdeII	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
NheI	GCTAGC	1 cut(s)	527
NlaIII	CATG	9 cut(s)	85, 690, 847, 1054, 1157, 1189, 1198, 1528, 1762
NlaIV	GGNNCC	4 cut(s)	259, 385, 790, 915
NmuCI	GTSAC	4 cut(s)	26, 1006, 1531, 1880
NsbI	TGCGCA	1 cut(s)	335
NspI	RCATGY	2 cut(s)	85, 1762
NspV	TTCGAA	2 cut(s)	176, 1138
PagI	TCATGA	2 cut(s)	1185, 1194
PciI	ACATGT	1 cut(s)	1758
PcsI	WCGNNNNNNNCGW	1 cut(s)	917
PctI	GAATGC	1 cut(s)	404
PfeI	GAWTC	6 cut(s)	188, 341, 553, 868, 875, 1021
PflMI	CCANNNNNTGG	1 cut(s)	1825
PkrI	GCNGC	6 cut(s)	49, 780, 1120, 1123, 1474, 1803
PleI	GAGTC	3 cut(s)	760, 981, 1192
PpsI	GAGTC	3 cut(s)	760, 981, 1192
Ppu21I	YACGTR	2 cut(s)	762, 1277
PpuMI	RGGWCCY	1 cut(s)	1180
PscI	ACATGT	1 cut(s)	1758
Psp1406I	AACGTT	1 cut(s)	1143
Psp5II	RGGWCCY	1 cut(s)	1180
Psp6I	CCWGG	1 cut(s)	1813
PspGI	CCWGG	1 cut(s)	1813
PspN4I	GGNNCC	4 cut(s)	259, 385, 790, 915
PspPI	GGNCC	2 cut(s)	914, 1180
PspPPI	RGGWCCY	1 cut(s)	1180
PsrI	GAACNNNNNNTAC	2 cut(s)	117, 149
PsuI	RGATCY	2 cut(s)	257, 1270
RsaI	GTAC	5 cut(s)	893, 1166, 1432, 1646, 1784
RsaNI	GTAC	5 cut(s)	892, 1165, 1431, 1645, 1783
RseI	CAYNNNNRTG	2 cut(s)	1400, 1529
SaqAI	TTAA	8 cut(s)	23, 62, 450, 558, 822, 1260, 1607, 1859
SatI	GCNGC	6 cut(s)	48, 779, 1119, 1122, 1473, 1802
Sau3AI	GATC	7 cut(s)	95, 257, 725, 751, 950, 1270, 1555
Sau96I	GGNCC	2 cut(s)	914, 1180
ScaI	AGTACT	1 cut(s)	1784
SchI	GAGTC	3 cut(s)	760, 982, 1192
ScrFI	CCNGG	1 cut(s)	1815
SduI	GDGCHC	1 cut(s)	1670
SfaNI	GCATC	5 cut(s)	321, 842, 925, 1977, 2014
SfuI	TTCGAA	2 cut(s)	176, 1138
SinI	GGWCC	1 cut(s)	1180
SmiMI	CAYNNNNRTG	2 cut(s)	1400, 1529
SmlI	CTYRAG	1 cut(s)	557
SmoI	CTYRAG	1 cut(s)	557
SnaBI	TACGTA	1 cut(s)	1277
SsiI	CCGC	3 cut(s)	48, 390, 1799
SspI	AATATT	2 cut(s)	1134, 1876
SspMI	CTAG	9 cut(s)	294, 456, 528, 548, 830, 1080, 1374, 1439, 1934
StyD4I	CCNGG	1 cut(s)	1813
StyI	CCWWGG	1 cut(s)	1050
TaiI	ACGT	6 cut(s)	764, 897, 1061, 1146, 1279, 1605
TatI	WGTACW	4 cut(s)	1164, 1430, 1644, 1782
TauI	GCSGC	1 cut(s)	50
TfiI	GAWTC	6 cut(s)	188, 341, 553, 868, 875, 1021
Tru1I	TTAA	8 cut(s)	23, 62, 450, 558, 822, 1260, 1607, 1859
Tru9I	TTAA	8 cut(s)	23, 62, 450, 558, 822, 1260, 1607, 1859
TscAI	CASTG	5 cut(s)	1015, 1233, 1722, 1749, 1828
TseFI	GTSAC	4 cut(s)	26, 1006, 1531, 1880
TseI	GCWGC	5 cut(s)	778, 1118, 1121, 1472, 1801
Tsp45I	GTSAC	4 cut(s)	26, 1006, 1531, 1880
TspDTI	ATGAA	9 cut(s)	132, 171, 653, 832, 1183, 1513, 1613, 1650, 1904
TspGWI	ACGGA	2 cut(s)	909, 1251
TspRI	CASTG	5 cut(s)	1015, 1233, 1722, 1749, 1828
Van91I	CCANNNNNTGG	1 cut(s)	1825
Vha464I	CTTAAG	1 cut(s)	557
VneI	GTGCAC	1 cut(s)	1666
VpaK11BI	GGWCC	1 cut(s)	1180
XapI	RAATTY	5 cut(s)	704, 839, 1364, 1654, 1721
XceI	RCATGY	2 cut(s)	85, 1762
XcmI	CCANNNNNNNNNTGG	2 cut(s)	745, 1057
XmiI	GTMKAC	1 cut(s)	1296
XspI	CTAG	9 cut(s)	294, 456, 528, 548, 830, 1080, 1374, 1439, 1934
ZrmI	AGTACT	1 cut(s)	1784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.