Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Forward (+)

68449148 .. 68453444

Gene Structure 4297 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ28023

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2253 bp

ATGAAAGCCGCAGAGAAAGCTTCAAAGGGCGGCGAGATAAGCATGTCGGAGAAGCAATCTGCCAATGGAGGTGAAGTTATAGTGGAACTTCAAAATGTTGGGACGTATGAAGGTAAAGGGTCTCCTTCAACCAAGCAAAGCAAAGTGAACTTGGGAGTGACCACTGAGTCTAGTAGTACCAGGTTCGGTGGGTTTCCTTCACTGGATGTTTCAAGAGCCAGTCCCAACCCCGGTAAGCCTCCGAGGCCCCCCGCCCGCAATGAGAGCACCAATAGGAGAAAGTCATTTAATAGGTCAGTTATCTCCAAACCCAAGTCAAGATTTGGTGAACCATCGCCTGTTCTGAATGAAGATGGTAGTTCAGATCATGTTGGTTCCCCATATAGGGGGGCTTCATTCAGTAGGGCTTCCCCAAATAACATATCAGGAGCTAGGGCCATATCCATCTCTTCTAATAGGAATGCGTCTCCAGGCAGGACCAAAGATAAGGAGGATGAAGAGATTTACAAGAAGGTTAAATTGAGTAGAGATAAGCACGGGAAAATGAACAAGATACTCTTGGCTGAGTTATTTGTTTTCCTGTGCATTTTGGGTTGTTTAGTGGCTAGCTTGACTGTGGAGAAACTAGAGAATTATATGGTTTGGGGCTTGGAGATTTGGAAATGGTGTGTGCTTGTCATGGTGATATTCAGTGGCATGTTGGTTACCAATTGGGTTATGCATTTGATAGTTTTTCTGATCGAGTGCAATTTTTTGCTTCGGAAGAAGGTTTTGTATTTTGTTCATGGAATGAAGAAGAGTGTTCAGGTTTTCTTGTGGTTGTCTCTGGTTCTTCTCACATGGCTACTGTTGTTCAATCGTGGGGTTAAGCGGTCAAAGACTACCACCAAGATTTTAAATTATGTTACATGGACTATTGTCTCACTTCTCATTGGGGCATTTTTATGGTTGTTAAAAACATTGTTGCTTAAGATCTTAGCATCTAGTTTCCATGTGAATACCTTCTTTGATAGAATTCAAGAATCAATCTTCCATCAGTATGTGCTTCAGACACTTTCGGGGCATCCGCTTATGGAGGAGGCTGATAGGAACGGTGGGAAATCACCGAGCACTAGCAAACTGAGTTACAGAGCGACAAAGAAGGCAAAGGCTGGAAAGGAAAAAGAGGTCATTGACATGACAAAGCTTCAACAGATAAAGCAAGAGAAGGTTTCTTCTTGGACCATGAAGGTCTTGGTGGATGCAGTAACTAGTTCAGGGCTGTCCACGATCTCCCATACATTGGATGAGATGGAGGGTCTGAATGAACAGAGAGATAAGGAGATTACCAGTGAGATGGAAGCAACTGCTGCTGCCTATGACATATTCCTTAACGTTGCTCCGCTGGGTGCTAATTACATTGAGGAGTGGGACCTCTTGAGGTTCATGATCGAGGAAGAAGTGGAGCTTGTGTGGCCGCTAATTGACGTAAATAAGACTGGACAAGTTGATAGAAAAACTCTAACAGAGTGGGTGGTGAAGGTCTACAATGGTCGCAAAGCATTAGCTCATTCATTAACCGACACCAAAACAGCCGTAAAACAATTGAACAAACTTGTGACTAGTGTCCTGGTTATTATTACCATTATAGTGTGGCTTCTGTTGATGGAAATTGCAACTACAAAAGTACTTGTCTTCCTCTCATCACAGCTCGTAGTGGCAGCTTTTATGTTTGGAAACACTTGCAAGACTATATTCGAAGCTATTATCTTCGTTTTTGTAATGCATCCATTTGACGTTGGTGACCGCTGCGTTATTGATGGCGTTCAGTTAATGGTTGAGGAGATGAACATCTTAACTACAGTCTTCTTAAAACTCAATCAAGAAAAGGTATACTATCCCAATTCAGTTTTATCCACAAAACCGATCAGCAATTATTACAGAAGTTCAAATATGGGTGATAATGTGGAGTTCTCAATTGCTTTTACTACACCAGTGAAGAAGATAGGCGAGCTGAGAGAGAAAGTAAAGGAGTATGTGGAGGGGAATCCGACGCTTTGGCATCCAAACCACCAAATTGTGGTGCTTGAGATCGAAAATGTGAATAAGTTAAAGCTGGCACTCTACTTCAGTCACACGATGAACTTTCAAGAATGGGGAGAAAAGCAAAAGCGGAGAAGTGAAATGGTCATGGCATTGAAGAAATGTTTGGAGGACCTACACATTACATACTATCTGCTGCCCCTAGAAGTTCGTCTCACTGAAACTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

750

Amino Acids

84.81

Weight (kDa)

9.24

Isoelectric Point (pI)

40.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Msy1	PF25886	188 - 330	5.4e-06	Mechanosensitive ion channel protein Msy1-like, transmembrane domain
MS_channel_2nd	PF00924	583 - 640	4.8e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	166
AccB7I	CCANNNNNTGG	2 cut(s)	1282, 2059
AccI	GTMKAC	2 cut(s)	1524, 1872
AclI	AACGTT	1 cut(s)	1374
AcoI	YGGCCR	1 cut(s)	1454
AcsI	RAATTY	1 cut(s)	1014
AcuI	CTGAAG	2 cut(s)	1031, 2092
AfaI	GTAC	2 cut(s)	178, 1668
AfiI	CCNNNNNNNGG	6 cut(s)	230, 384, 385, 386, 1282, 2059
AflII	CTTAAG	1 cut(s)	968
AhlI	ACTAGT	2 cut(s)	1250, 1601
AjnI	CCWGG	3 cut(s)	179, 469, 1608
AjuI	GAANNNNNNNTTGG	2 cut(s)	2171, 2203
Alw21I	GWGCWC	2 cut(s)	269, 1112
Alw26I	GTCTC	5 cut(s)	126, 471, 828, 925, 2240
AoxI	GGCC	3 cut(s)	245, 435, 1454
ApeKI	GCWGC	5 cut(s)	1349, 1352, 1700, 1788, 2218
ApoI	RAATTY	1 cut(s)	1014
Asp700I	GAANNNNTTC	1 cut(s)	1001
AspS9I	GGNCC	6 cut(s)	246, 435, 477, 1221, 1411, 2194
AsuC2I	CCSGG	1 cut(s)	231
AsuHPI	GGTGA	7 cut(s)	83, 338, 694, 1095, 1528, 1793, 1949
AsuII	TTCGAA	1 cut(s)	1737
AsuNHI	GCTAGC	1 cut(s)	605
AvaII	GGWCC	4 cut(s)	477, 1221, 1411, 2194
BbsI	GAAGAC	2 cut(s)	1666, 1837
Bbv12I	GWGCWC	2 cut(s)	269, 1112
BbvI	GCAGC	5 cut(s)	1336, 1339, 1712, 1775, 2205
BccI	CCATC	8 cut(s)	340, 347, 452, 1041, 1285, 1330, 1639, 1793
BceAI	ACGGC	1 cut(s)	1559
BciT130I	CCWGG	3 cut(s)	181, 471, 1610
BcnI	CCSGG	1 cut(s)	231
BcoDI	GTCTC	5 cut(s)	126, 471, 828, 925, 2240
BcuI	ACTAGT	2 cut(s)	1250, 1601
BfaI	CTAG	9 cut(s)	171, 432, 606, 626, 984, 1113, 1251, 1602, 2225
BfmI	CTRYAG	1 cut(s)	1839
BfrI	CTTAAG	1 cut(s)	968
BglI	GCCNNNNNGGC	1 cut(s)	244
BglII	AGATCT	1 cut(s)	972
BisI	GCNGC	8 cut(s)	9, 31, 1350, 1353, 1457, 1701, 1789, 2219
BlsI	GCNGC	8 cut(s)	10, 32, 1351, 1354, 1458, 1702, 1790, 2220
BmcAI	AGTACT	1 cut(s)	1668
Bme1390I	CCNGG	4 cut(s)	181, 231, 471, 1610
Bme18I	GGWCC	4 cut(s)	477, 1221, 1411, 2194
BmgT120I	GGNCC	6 cut(s)	246, 435, 477, 1221, 1411, 2194
BmiI	GGNNCC	3 cut(s)	248, 376, 1412
BmrFI	CCNGG	4 cut(s)	181, 231, 471, 1610
BmsI	GCATC	5 cut(s)	989, 1072, 1231, 1774, 2050
BmtI	GCTAGC	1 cut(s)	609
BoxI	GACNNNNGTC	2 cut(s)	917, 1604
BpiI	GAAGAC	2 cut(s)	1666, 1837
BpmI	CTGGAG	1 cut(s)	453
Bpu14I	TTCGAA	1 cut(s)	1737
BpuEI	CTTGAG	2 cut(s)	1438, 2087
BpuMI	CCSGG	1 cut(s)	231
BsaBI	GATNNNNATC	1 cut(s)	1829
BsaI	GGTCTC	1 cut(s)	126
BsaJI	CCNNGG	2 cut(s)	229, 242
BsaXI	ACNNNNNCTCC	4 cut(s)	60, 90, 1436, 1466
Bsc4I	CCNNNNNNNGG	6 cut(s)	230, 384, 385, 386, 1282, 2059
Bse1I	ACTGG	5 cut(s)	207, 219, 1329, 1483, 1973
Bse3DI	GCAATG	1 cut(s)	265
Bse8I	GATNNNNATC	1 cut(s)	1829
BseBI	CCWGG	3 cut(s)	181, 471, 1610
BseDI	CCNNGG	2 cut(s)	229, 242
BseGI	GGATG	7 cut(s)	211, 499, 1063, 1246, 1291, 1765, 2041
BseJI	GATNNNNATC	1 cut(s)	1829
BseLI	CCNNNNNNNGG	6 cut(s)	230, 384, 385, 386, 1282, 2059
BseMI	GCAATG	1 cut(s)	265
BseMII	CTCAG	4 cut(s)	156, 555, 1112, 1985
BseNI	ACTGG	5 cut(s)	207, 219, 1329, 1483, 1973
BseRI	GAGGAG	3 cut(s)	1091, 1418, 1835
BseXI	GCAGC	5 cut(s)	1336, 1339, 1712, 1775, 2205
BseYI	CCCAGC	1 cut(s)	1384
BshFI	GGCC	3 cut(s)	247, 437, 1456
BsiHKAI	GWGCWC	2 cut(s)	269, 1112
BsiSI	CCGG	1 cut(s)	231
BslFI	GGGAC	3 cut(s)	115, 207, 1424
BslI	CCNNNNNNNGG	6 cut(s)	230, 384, 385, 386, 1282, 2059
BsmAI	GTCTC	5 cut(s)	126, 471, 828, 925, 2240
BsmBI	CGTCTC	2 cut(s)	471, 2240
BsmFI	GGGAC	3 cut(s)	115, 207, 1424
BsmI	GAATGC	1 cut(s)	466
BsnI	GGCC	3 cut(s)	247, 437, 1456
Bso31I	GGTCTC	1 cut(s)	126
Bsp119I	TTCGAA	1 cut(s)	1737
Bsp1286I	GDGCHC	2 cut(s)	269, 1112
Bsp143I	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
BspANI	GGCC	3 cut(s)	247, 437, 1456
BspCNI	CTCAG	4 cut(s)	157, 556, 1113, 1986
BspHI	TCATGA	1 cut(s)	1425
BspLI	GGNNCC	3 cut(s)	248, 376, 1412
BspOI	GCTAGC	1 cut(s)	609
BspT104I	TTCGAA	1 cut(s)	1737
BspTI	CTTAAG	1 cut(s)	968
BspTNI	GGTCTC	1 cut(s)	126
BsrDI	GCAATG	1 cut(s)	265
BsrI	ACTGG	5 cut(s)	207, 219, 1329, 1483, 1973
BssECI	CCNNGG	2 cut(s)	229, 242
BssMI	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
BssNAI	GTATAC	1 cut(s)	1873
Bst1107I	GTATAC	1 cut(s)	1873
Bst2UI	CCWGG	3 cut(s)	181, 471, 1610
Bst4CI	ACNGT	4 cut(s)	616, 849, 1094, 1843
Bst6I	CTCTTC	3 cut(s)	454, 492, 791
BstAFI	CTTAAG	1 cut(s)	968
BstAPI	GCANNNNNTGC	1 cut(s)	1349
BstBI	TTCGAA	1 cut(s)	1737
BstC8I	GCNNGC	4 cut(s)	256, 607, 1991, 2097
BstDEI	CTNAG	5 cut(s)	165, 564, 976, 1121, 1994
BstEII	GGTNACC	2 cut(s)	703, 1781
BstF5I	GGATG	7 cut(s)	211, 499, 1063, 1246, 1291, 1765, 2041
BstKTI	GATC	7 cut(s)	367, 741, 975, 1272, 1431, 1908, 2073
BstMAI	GTCTC	5 cut(s)	126, 471, 828, 925, 2240
BstMBI	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
BstMWI	GCNNNNNNNGC	6 cut(s)	17, 39, 244, 264, 1349, 1453
BstNI	CCWGG	3 cut(s)	181, 471, 1610
BstNSI	RCATGY	2 cut(s)	46, 700
BstPAI	GACNNNNGTC	2 cut(s)	917, 1604
BstPI	GGTNACC	2 cut(s)	703, 1781
BstSCI	CCNGG	4 cut(s)	179, 229, 469, 1608
BstSFI	CTRYAG	1 cut(s)	1839
BstV1I	GCAGC	5 cut(s)	1336, 1339, 1712, 1775, 2205
BstV2I	GAAGAC	2 cut(s)	1666, 1837
BstX2I	RGATCY	1 cut(s)	972
BstXI	CCANNNNNNTGG	1 cut(s)	1336
BstYI	RGATCY	1 cut(s)	972
BstZ17I	GTATAC	1 cut(s)	1873
BsuRI	GGCC	3 cut(s)	247, 437, 1456
BtgZI	GCGATG	1 cut(s)	318
BtsCI	GGATG	7 cut(s)	211, 499, 1063, 1246, 1291, 1765, 2041
BtsIMutI	CAGTG	6 cut(s)	162, 200, 697, 1336, 1980, 2238
Cac8I	GCNNGC	4 cut(s)	256, 607, 1991, 2097
CciI	TCATGA	1 cut(s)	1425
Cfr13I	GGNCC	6 cut(s)	246, 435, 477, 1221, 1411, 2194
CseI	GACGC	2 cut(s)	453, 2041
CsiI	ACCWGGT	1 cut(s)	179
Csp6I	GTAC	2 cut(s)	177, 1667
CviQI	GTAC	2 cut(s)	177, 1667
DdeI	CTNAG	5 cut(s)	165, 564, 976, 1121, 1994
DpnI	GATC	7 cut(s)	366, 740, 974, 1271, 1430, 1907, 2072
DpnII	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
DraI	TTTAAA	1 cut(s)	897
DrdI	GACNNNNNNGTC	1 cut(s)	166
DseDI	GACNNNNNNGTC	1 cut(s)	166
EaeI	YGGCCR	1 cut(s)	1454
Eam1104I	CTCTTC	3 cut(s)	454, 492, 791
EarI	CTCTTC	3 cut(s)	454, 492, 791
Eco31I	GGTCTC	1 cut(s)	126
Eco47I	GGWCC	4 cut(s)	477, 1221, 1411, 2194
Eco57I	CTGAAG	2 cut(s)	1031, 2092
Eco91I	GGTNACC	2 cut(s)	703, 1781
EcoO109I	RGGNCCY	3 cut(s)	246, 1411, 2194
EcoO65I	GGTNACC	2 cut(s)	703, 1781
EcoRI	GAATTC	1 cut(s)	1014
EcoRII	CCWGG	3 cut(s)	179, 469, 1608
EcoT22I	ATGCAT	2 cut(s)	723, 1767
Esp3I	CGTCTC	2 cut(s)	471, 2240
FalI	AAGNNNNNCTT	4 cut(s)	542, 574, 1431, 1463
FaqI	GGGAC	3 cut(s)	115, 207, 1424
FauI	CCCGC	2 cut(s)	259, 263
FblI	GTMKAC	2 cut(s)	1524, 1872
Fnu4HI	GCNGC	8 cut(s)	9, 31, 1350, 1353, 1457, 1701, 1789, 2219
FokI	GGATG	7 cut(s)	218, 506, 1050, 1253, 1298, 1752, 2028
Fsp4HI	GCNGC	8 cut(s)	9, 31, 1350, 1353, 1457, 1701, 1789, 2219
FspBI	CTAG	9 cut(s)	171, 432, 606, 626, 984, 1113, 1251, 1602, 2225
GluI	GCNGC	8 cut(s)	9, 31, 1350, 1353, 1457, 1701, 1789, 2219
GsaI	CCCAGC	1 cut(s)	1388
GsuI	CTGGAG	1 cut(s)	453
HaeIII	GGCC	3 cut(s)	247, 437, 1456
HapII	CCGG	1 cut(s)	231
HgaI	GACGC	2 cut(s)	453, 2041
HindIII	AAGCTT	2 cut(s)	18, 1184
HinfI	GANTC	3 cut(s)	167, 1022, 2026
HpaII	CCGG	1 cut(s)	231
HphI	GGTGA	7 cut(s)	83, 338, 694, 1095, 1528, 1793, 1949
Hpy166II	GTNNAC	5 cut(s)	148, 329, 1266, 1525, 1873
Hpy188I	TCNGA	9 cut(s)	49, 243, 345, 364, 738, 762, 1050, 1302, 2031
Hpy188III	TCNNGA	8 cut(s)	213, 318, 426, 1019, 1417, 1426, 1862, 2129
Hpy8I	GTNNAC	5 cut(s)	148, 329, 1266, 1525, 1873
Hpy99I	CGWCG	1 cut(s)	2035
HpyCH4III	ACNGT	4 cut(s)	616, 849, 1094, 1843
HpyCH4IV	ACGT	4 cut(s)	104, 1374, 1467, 1776
HpyCH4V	TGCA	7 cut(s)	585, 721, 747, 1244, 1655, 1725, 1765
HpyF10VI	GCNNNNNNNGC	6 cut(s)	17, 39, 244, 264, 1349, 1453
HpyF3I	CTNAG	5 cut(s)	165, 564, 976, 1121, 1994
HpySE526I	ACGT	4 cut(s)	104, 1374, 1467, 1776
Kzo9I	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
LmnI	GCTCC	3 cut(s)	428, 1384, 1444
Lsp1109I	GCAGC	5 cut(s)	1336, 1339, 1712, 1775, 2205
LweI	GCATC	5 cut(s)	989, 1072, 1231, 1774, 2050
MabI	ACCWGGT	1 cut(s)	179
MaeI	CTAG	9 cut(s)	171, 432, 606, 626, 984, 1113, 1251, 1602, 2225
MaeII	ACGT	4 cut(s)	104, 1374, 1467, 1776
MaeIII	GTNAC	8 cut(s)	157, 703, 904, 1124, 1246, 1597, 1781, 2111
MalI	GATC	7 cut(s)	366, 740, 974, 1271, 1430, 1907, 2072
MboI	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
MfeI	CAATTG	3 cut(s)	709, 1583, 1956
MflI	RGATCY	1 cut(s)	972
MhlI	GDGCHC	2 cut(s)	269, 1112
MlyI	GAGTC	1 cut(s)	176
MmeI	TCCRAC	2 cut(s)	27, 2054
Mph1103I	ATGCAT	2 cut(s)	723, 1767
MroXI	GAANNNNTTC	1 cut(s)	1001
MslI	CAYNNNNRTG	4 cut(s)	943, 1038, 1175, 1628
MspA1I	CMGCKG	2 cut(s)	1384, 1788
MspCI	CTTAAG	1 cut(s)	968
MspI	CCGG	1 cut(s)	231
MspR9I	CCNGG	4 cut(s)	181, 231, 471, 1610
MunI	CAATTG	3 cut(s)	709, 1583, 1956
Mva1269I	GAATGC	1 cut(s)	466
MvaI	CCWGG	3 cut(s)	181, 471, 1610
MwoI	GCNNNNNNNGC	6 cut(s)	17, 39, 244, 264, 1349, 1453
NciI	CCSGG	1 cut(s)	231
NdeII	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
NheI	GCTAGC	1 cut(s)	605
NlaIV	GGNNCC	3 cut(s)	248, 376, 1412
NmuCI	GTSAC	4 cut(s)	157, 1597, 1781, 2111
NsiI	ATGCAT	2 cut(s)	723, 1767
NspI	RCATGY	2 cut(s)	46, 700
NspV	TTCGAA	1 cut(s)	1737
PagI	TCATGA	1 cut(s)	1425
PctI	GAATGC	1 cut(s)	466
PdmI	GAANNNNTTC	1 cut(s)	1001
PfeI	GAWTC	2 cut(s)	1022, 2026
PflMI	CCANNNNNTGG	2 cut(s)	1282, 2059
PkrI	GCNGC	8 cut(s)	10, 32, 1351, 1354, 1458, 1702, 1790, 2220
PleI	GAGTC	1 cut(s)	175
PpsI	GAGTC	1 cut(s)	175
PpuMI	RGGWCCY	2 cut(s)	1411, 2194
PshAI	GACNNNNGTC	2 cut(s)	917, 1604
Psp1406I	AACGTT	1 cut(s)	1374
Psp5II	RGGWCCY	2 cut(s)	1411, 2194
Psp6I	CCWGG	3 cut(s)	179, 469, 1608
PspEI	GGTNACC	2 cut(s)	703, 1781
PspFI	CCCAGC	1 cut(s)	1384
PspGI	CCWGG	3 cut(s)	179, 469, 1608
PspN4I	GGNNCC	3 cut(s)	248, 376, 1412
PspPI	GGNCC	6 cut(s)	246, 435, 477, 1221, 1411, 2194
PspPPI	RGGWCCY	2 cut(s)	1411, 2194
PsuI	RGATCY	1 cut(s)	972
RsaI	GTAC	2 cut(s)	178, 1668
RsaNI	GTAC	2 cut(s)	177, 1667
RseI	CAYNNNNRTG	4 cut(s)	943, 1038, 1175, 1628
SatI	GCNGC	8 cut(s)	9, 31, 1350, 1353, 1457, 1701, 1789, 2219
Sau3AI	GATC	7 cut(s)	364, 738, 972, 1269, 1428, 1905, 2070
Sau96I	GGNCC	6 cut(s)	246, 435, 477, 1221, 1411, 2194
ScaI	AGTACT	1 cut(s)	1668
SchI	GAGTC	1 cut(s)	176
ScrFI	CCNGG	4 cut(s)	181, 231, 471, 1610
SduI	GDGCHC	2 cut(s)	269, 1112
SexAI	ACCWGGT	1 cut(s)	179
SfaNI	GCATC	5 cut(s)	989, 1072, 1231, 1774, 2050
SfcI	CTRYAG	1 cut(s)	1839
SfuI	TTCGAA	1 cut(s)	1737
SinI	GGWCC	4 cut(s)	477, 1221, 1411, 2194
SmiMI	CAYNNNNRTG	4 cut(s)	943, 1038, 1175, 1628
SmlI	CTYRAG	3 cut(s)	968, 1417, 2066
SmoI	CTYRAG	3 cut(s)	968, 1417, 2066
SpeI	ACTAGT	2 cut(s)	1250, 1601
SspMI	CTAG	9 cut(s)	171, 432, 606, 626, 984, 1113, 1251, 1602, 2225
StyD4I	CCNGG	4 cut(s)	179, 229, 469, 1608
TaaI	ACNGT	4 cut(s)	616, 849, 1094, 1843
TaiI	ACGT	4 cut(s)	107, 1377, 1470, 1779
TaqI	TCGA	4 cut(s)	741, 1431, 1737, 2073
TatI	WGTACW	1 cut(s)	1666
TauI	GCSGC	3 cut(s)	11, 33, 1459
TfiI	GAWTC	2 cut(s)	1022, 2026
TscAI	CASTG	6 cut(s)	169, 207, 697, 1336, 1980, 2245
TseFI	GTSAC	4 cut(s)	157, 1597, 1781, 2111
TseI	GCWGC	5 cut(s)	1349, 1352, 1700, 1788, 2218
Tsp45I	GTSAC	4 cut(s)	157, 1597, 1781, 2111
TspRI	CASTG	6 cut(s)	169, 207, 697, 1336, 1980, 2245
Van91I	CCANNNNNTGG	2 cut(s)	1282, 2059
Vha464I	CTTAAG	1 cut(s)	968
VpaK11BI	GGWCC	4 cut(s)	477, 1221, 1411, 2194
XapI	RAATTY	1 cut(s)	1014
XceI	RCATGY	2 cut(s)	46, 700
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1231
XmiI	GTMKAC	2 cut(s)	1524, 1872
XmnI	GAANNNNTTC	1 cut(s)	1001
XspI	CTAG	9 cut(s)	171, 432, 606, 626, 984, 1113, 1251, 1602, 2225
ZrmI	AGTACT	1 cut(s)	1668
Zsp2I	ATGCAT	2 cut(s)	723, 1767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.