Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

1311763 .. 1315153

Gene Structure 3391 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00157480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGCAAGCTTTCCTTCGCTTATTAAGTGACAGGTTCTTGATGGAAGCCGCAGAGAAAGGCTTAAAAGGAAGCAGAGTAAGCATGTCGAAAAATCGATCTTGGAATGATGGAGGTGATGAAGTTGTAGTGGTGGTTCAAAGCGAAGAGATGGAAGATGAAGCAAAAGGGTCTTGTTCGAAAGGCTTAGAATCTTCAGCTCCCAATCAAATTAGCAAGGTGGAATTGCAGGTTGGTTCTCATTCCCCTGAGATTACAGGATCCAGCAACGACCCCATCCCCAATGAGACCCTCACTAGGAGAAAGTCATCTATCTGTCTTGGTCAACAGTCGATGCGCATTGATTCTGATATTCTTGAAGAGGACACTAATTTAGAGCAGGATGGTTCCCCCGCAAATAAATCAGCATATAAAGAGGAGGAGGAGGAGGAGGAGGAGGAGATTTACGAGAAGGTTAATTCTTGCAGGAAAGTGAACAAGAAAATGCTGATGGAGTATATTGTTTTCGCGTGCAGTTTGGGTGTTATAGTAGCTAGCTTTACTGTCGAGAAACTAGAGAATCTTAAGGTGTGGGGATTGGAGATGTGGAAATTGTGTGTGCTTGTTATGCTCATATTCTGTGGAATGCTGGTTACGAATTGTGTTTTGACTCTGTTAGTTTTTGTGATTGAGAGGAACTTTCTGCTTACGAGGAAGAAGGAGGTGTTGTATTTGGTTTATGGAATGAAAAAGAGTGTTCAGGTTTTCCTGTGGTTCGGTATGGTGCTTCTCACATGGCTATTCTTTTTCAAATTCAATCGTGGCAATGCGAAATCAAAGACTTCCACCAAGATTTTGGATCATATTACGTGGACTCTTGTTTCGCTGCTTATTGGTGCCTTTTTGTGGATGTTATCAACTTTGTTGTTTAAGACCTTAGCATCTAAATTCATGGCCGTCGCCTATTTTGATAGGATTCAAGAATCTTTGTTCCATCAGTACGTTATTGAGACACTTTCGGGGCCTCCGTTAGTAGAGGAGGATGCCAAGATTAGGAGATCACTCAGCACAGGGTTTGTGAGTCTCAGGACGATAAACAATGTGGAAAAGAAGGTCACTGGTAAGGTGAATTCTGCAAGGAAGGTTTCAGTTGAGACCATGGATACGTTGGTGGGTGCAGTTTCTACTAGAGGTACAGAGATTACAAGTGAGTGGGAAGCCAAAGCTGCTGCCTTCAAAATATTTCGAAACGTTGCTAAGCATGGTAACAAGTACATCGAGGAGGAGGACCTCATGAGGTTCATGACTCGGGAAGAGGTGGCTAATGTATTTCCACTGTTTGACGGAGCAATGGAAAACGGCAAAATTAAAAGAAAAGATCTACAAAGTTGGATGGTAAATGTCTACCATGAACGCAAAGCATTAGCTTTTTCTATGTCTGACACCAAAACAGTTCTAAAGCAATTTGAGAATTTTTTTACTAGCTTGGTGATTGTTATTACCATCGTAGTGTGGCTACTGTTGGTGGGAATTGCAAGTACAAACCTAGTTGTTTTCCTCTCAACACAGTTCTTATTGGCAGCTTTTATTTTTGGAAACACTTGCAAGAATGTATTTGAAGCTATCGTGTTCATGTTTGTGACACATCCATTTGATGTTGGTGATCGCATTGTTGTTGATGATGTTCCGTTGATCGTTGAAGAGATGAACGTCTTAACTACCACCTTCTTGAAACCAAATAATGAAAAGGTGTACTATCCAAATTCAGTTTTGTGCACCAAAATCATCAGCAATTATTACAGAAGTCCAGAAATGGGAGATACAGTGGAATTCTCAATTATTTTGACACCAGTGGAGAAGATCGACACGCTGAGAAAGAGAATAAAGGAGTACTTGGAGAGCAAACCGCAGCATTGGCGTCCAGGCCACAGTGTGGTGGTGATTGATATCGAAGATGTGAATAAGTTAAAGATGGCACTCAATATTTGTCACACTATGAACTTTCAAGAAATTGGTGAAAGGAATAAACGGCGAACTGAGCTAGTCATCGAATTGATGAGAACTTTGGAAGACCTAAACATCACATACAGTCTGCTGCCCCAAGAAGTTCATCTCACTCAAAATAAAGCAGATGCAAACGTTTCCTTCAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

710

Amino Acids

81.1

Weight (kDa)

6.31

Isoelectric Point (pI)

41.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	528 - 592	4.3e-11	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	335
Acc36I	ACCTGC	1 cut(s)	217
AccB1I	GGYRCC	1 cut(s)	872
AccB7I	CCANNNNNTGG	1 cut(s)	1909
AccI	GTMKAC	1 cut(s)	1380
AccII	CGCG	1 cut(s)	506
AciI	CCGC	3 cut(s)	48, 390, 1883
AclI	AACGTT	2 cut(s)	1227, 2115
AclWI	GGATC	3 cut(s)	252, 265, 843
AcoI	YGGCCR	1 cut(s)	930
AcsI	RAATTY	6 cut(s)	788, 923, 1105, 1447, 1738, 1805
AcuI	CTGAAG	1 cut(s)	177
AcyI	GRCGYC	1 cut(s)	1894
AdeI	CACNNNGTG	1 cut(s)	1909
AfaI	GTAC	6 cut(s)	977, 1171, 1250, 1516, 1730, 1868
AfiI	CCNNNNNNNGG	5 cut(s)	294, 882, 1029, 1790, 1909
AflII	CTTAAG	1 cut(s)	560
AjnI	CCWGG	1 cut(s)	1897
AjuI	GAANNNNNNNTTGG	2 cut(s)	213, 245
Alw21I	GWGCWC	1 cut(s)	1754
Alw26I	GTCTC	4 cut(s)	278, 980, 1064, 1124
Alw44I	GTGCAC	1 cut(s)	1750
AlwI	GGATC	3 cut(s)	252, 265, 843
Ama87I	CYCGRG	1 cut(s)	1284
AoxI	GGCC	3 cut(s)	930, 998, 1900
ApaLI	GTGCAC	1 cut(s)	1750
ApeKI	GCWGC	6 cut(s)	862, 1202, 1205, 1556, 1885, 2071
ApoI	RAATTY	6 cut(s)	788, 923, 1105, 1447, 1738, 1805
ArsI	GACNNNNNNTTYG	2 cut(s)	841, 873
AspLEI	GCGC	1 cut(s)	336
AspS9I	GGNCC	2 cut(s)	998, 1264
AsuHPI	GGTGA	6 cut(s)	125, 1114, 1477, 1649, 1927, 2003
AsuII	TTCGAA	2 cut(s)	176, 1222
AsuNHI	GCTAGC	1 cut(s)	530
AvaI	CYCGRG	1 cut(s)	1284
AvaII	GGWCC	1 cut(s)	1264
BaeGI	GKGCMC	1 cut(s)	1754
BaeI	ACNNNNGTAYC	2 cut(s)	1131, 1164
BamHI	GGATCC	1 cut(s)	257
BanI	GGYRCC	1 cut(s)	872
BbsI	GAAGAC	1 cut(s)	2052
Bbv12I	GWGCWC	1 cut(s)	1754
BbvI	GCAGC	6 cut(s)	849, 1189, 1192, 1568, 1897, 2058
BceAI	ACGGC	3 cut(s)	917, 1351, 2021
BciT130I	CCWGG	1 cut(s)	1899
BciVI	GTATCC	1 cut(s)	1132
BcoDI	GTCTC	4 cut(s)	278, 980, 1064, 1124
BfaI	CTAG	7 cut(s)	294, 531, 551, 1164, 1458, 1523, 2018
BfrI	CTTAAG	1 cut(s)	560
BfuAI	ACCTGC	1 cut(s)	217
BfuI	GTATCC	1 cut(s)	1132
BglII	AGATCT	1 cut(s)	1354
BisI	GCNGC	7 cut(s)	48, 863, 1203, 1206, 1557, 1886, 2072
BlpI	GCTNAGC	1 cut(s)	1233
BlsI	GCNGC	7 cut(s)	49, 864, 1204, 1207, 1558, 1887, 2073
BmcAI	AGTACT	1 cut(s)	1868
Bme1390I	CCNGG	1 cut(s)	1899
Bme18I	GGWCC	1 cut(s)	1264
BmeT110I	CYCGRG	1 cut(s)	1284
BmgT120I	GGNCC	2 cut(s)	998, 1264
BmiI	GGNNCC	4 cut(s)	259, 385, 874, 999
BmrFI	CCNGG	1 cut(s)	1899
BmsI	GCATC	4 cut(s)	321, 926, 1009, 2098
BmtI	GCTAGC	1 cut(s)	534
BpiI	GAAGAC	1 cut(s)	2052
Bpu10I	CCTNAGC	1 cut(s)	913
Bpu1102I	GCTNAGC	1 cut(s)	1233
Bpu14I	TTCGAA	2 cut(s)	176, 1222
Bsa29I	ATCGAT	1 cut(s)	94
BsaAI	YACGTR	1 cut(s)	846
BsaBI	GATNNNNATC	1 cut(s)	1922
BsaHI	GRCGYC	1 cut(s)	1894
BsaI	GGTCTC	2 cut(s)	278, 1124
BsaJI	CCNNGG	1 cut(s)	1134
BsaXI	ACNNNNNCTCC	4 cut(s)	689, 719, 1785, 1815
Bsc4I	CCNNNNNNNGG	5 cut(s)	294, 882, 1029, 1790, 1909
Bse1I	ACTGG	2 cut(s)	1099, 1826
Bse3DI	GCAATG	2 cut(s)	808, 1332
Bse8I	GATNNNNATC	1 cut(s)	1922
BseBI	CCWGG	1 cut(s)	1899
BseCI	ATCGAT	1 cut(s)	94
BseDI	CCNNGG	1 cut(s)	1134
BseGI	GGATG	6 cut(s)	273, 385, 891, 1024, 1374, 1621
BseJI	GATNNNNATC	1 cut(s)	1922
BseLI	CCNNNNNNNGG	5 cut(s)	294, 882, 1029, 1790, 1909
BseMI	GCAATG	2 cut(s)	808, 1332
BseMII	CTCAG	5 cut(s)	237, 1054, 1075, 1838, 2004
BseNI	ACTGG	2 cut(s)	1099, 1826
BseSI	GKGCMC	1 cut(s)	1754
BseXI	GCAGC	6 cut(s)	849, 1189, 1192, 1568, 1897, 2058
BsgI	GTGCAG	2 cut(s)	529, 1173
Bsh1236I	CGCG	1 cut(s)	506
BshFI	GGCC	3 cut(s)	932, 1000, 1902
BshNI	GGYRCC	1 cut(s)	872
BshVI	ATCGAT	1 cut(s)	94
BsiHKAI	GWGCWC	1 cut(s)	1754
BsiHKCI	CYCGRG	1 cut(s)	1284
BslI	CCNNNNNNNGG	5 cut(s)	294, 882, 1029, 1790, 1909
BsmAI	GTCTC	4 cut(s)	278, 980, 1064, 1124
BsmI	GAATGC	1 cut(s)	627
BsnI	GGCC	3 cut(s)	932, 1000, 1902
Bso31I	GGTCTC	2 cut(s)	278, 1124
BsoBI	CYCGRG	1 cut(s)	1284
Bsp119I	TTCGAA	2 cut(s)	176, 1222
Bsp1286I	GDGCHC	1 cut(s)	1754
Bsp143I	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
Bsp1720I	GCTNAGC	1 cut(s)	1233
Bsp19I	CCATGG	1 cut(s)	1134
BspACI	CCGC	3 cut(s)	48, 390, 1883
BspANI	GGCC	3 cut(s)	932, 1000, 1902
BspCNI	CTCAG	5 cut(s)	238, 1053, 1074, 1839, 2005
BspDI	ATCGAT	1 cut(s)	94
BspFNI	CGCG	1 cut(s)	506
BspHI	TCATGA	2 cut(s)	1269, 1278
BspLI	GGNNCC	4 cut(s)	259, 385, 874, 999
BspMI	ACCTGC	1 cut(s)	217
BspOI	GCTAGC	1 cut(s)	534
BspPI	GGATC	3 cut(s)	252, 265, 843
BspT104I	TTCGAA	2 cut(s)	176, 1222
BspT107I	GGYRCC	1 cut(s)	872
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	2 cut(s)	278, 1124
BsrDI	GCAATG	2 cut(s)	808, 1332
BsrI	ACTGG	2 cut(s)	1099, 1826
BssECI	CCNNGG	1 cut(s)	1134
BssMI	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
BssNI	GRCGYC	1 cut(s)	1894
BssT1I	CCWWGG	1 cut(s)	1134
Bst2UI	CCWGG	1 cut(s)	1899
Bst4CI	ACNGT	9 cut(s)	327, 541, 1314, 1429, 1497, 1545, 1801, 1907, 2066
Bst6I	CTCTTC	4 cut(s)	138, 351, 1284, 1671
BstACI	GRCGYC	1 cut(s)	1894
BstAFI	CTTAAG	1 cut(s)	560
BstBAI	YACGTR	1 cut(s)	846
BstBI	TTCGAA	2 cut(s)	176, 1222
BstC8I	GCNNGC	3 cut(s)	6, 508, 532
BstDEI	CTNAG	8 cut(s)	184, 246, 913, 1040, 1061, 1233, 1847, 2013
BstDSI	CCRYGG	1 cut(s)	1134
BstF5I	GGATG	6 cut(s)	273, 385, 891, 1024, 1374, 1621
BstFNI	CGCG	1 cut(s)	506
BstHHI	GCGC	1 cut(s)	336
BstKTI	GATC	8 cut(s)	98, 260, 838, 1037, 1357, 1642, 1671, 1839
BstMAI	GTCTC	4 cut(s)	278, 980, 1064, 1124
BstMBI	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
BstMWI	GCNNNNNNNGC	5 cut(s)	78, 604, 1202, 1891, 2014
BstNI	CCWGG	1 cut(s)	1899
BstNSI	RCATGY	1 cut(s)	85
BstSCI	CCNGG	1 cut(s)	1897
BstSLI	GKGCMC	1 cut(s)	1754
BstUI	CGCG	1 cut(s)	506
BstV1I	GCAGC	6 cut(s)	849, 1189, 1192, 1568, 1897, 2058
BstV2I	GAAGAC	1 cut(s)	2052
BstX2I	RGATCY	2 cut(s)	257, 1354
BstXI	CCANNNNNNTGG	1 cut(s)	832
BstYI	RGATCY	2 cut(s)	257, 1354
Bsu15I	ATCGAT	1 cut(s)	94
BsuI	GTATCC	1 cut(s)	1132
BsuRI	GGCC	3 cut(s)	932, 1000, 1902
BsuTUI	ATCGAT	1 cut(s)	94
BtgI	CCRYGG	1 cut(s)	1134
BtsCI	GGATG	6 cut(s)	273, 385, 891, 1024, 1374, 1621
BtsIMutI	CAGTG	5 cut(s)	1092, 1310, 1806, 1833, 1912
BveI	ACCTGC	1 cut(s)	217
Cac8I	GCNNGC	3 cut(s)	6, 508, 532
CciI	TCATGA	2 cut(s)	1269, 1278
CfoI	GCGC	1 cut(s)	336
Cfr13I	GGNCC	2 cut(s)	998, 1264
ClaI	ATCGAT	1 cut(s)	94
CseI	GACGC	1 cut(s)	1883
Csp6I	GTAC	6 cut(s)	976, 1170, 1249, 1515, 1729, 1867
CviAII	CATG	9 cut(s)	82, 771, 928, 1135, 1238, 1270, 1279, 1385, 1609
CviQI	GTAC	6 cut(s)	976, 1170, 1249, 1515, 1729, 1867
DdeI	CTNAG	8 cut(s)	184, 246, 913, 1040, 1061, 1233, 1847, 2013
DpnI	GATC	8 cut(s)	97, 259, 837, 1036, 1356, 1641, 1670, 1838
DpnII	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
DraIII	CACNNNGTG	1 cut(s)	1909
EaeI	YGGCCR	1 cut(s)	930
Eam1104I	CTCTTC	4 cut(s)	138, 351, 1284, 1671
EarI	CTCTTC	4 cut(s)	138, 351, 1284, 1671
Eco130I	CCWWGG	1 cut(s)	1134
Eco31I	GGTCTC	2 cut(s)	278, 1124
Eco32I	GATATC	1 cut(s)	1924
Eco47I	GGWCC	1 cut(s)	1264
Eco57I	CTGAAG	1 cut(s)	177
Eco88I	CYCGRG	1 cut(s)	1284
EcoO109I	RGGNCCY	2 cut(s)	998, 1264
EcoRI	GAATTC	2 cut(s)	1105, 1805
EcoRII	CCWGG	1 cut(s)	1897
EcoRV	GATATC	1 cut(s)	1924
EcoT14I	CCWWGG	1 cut(s)	1134
ErhI	CCWWGG	1 cut(s)	1134
FaeI	CATG	9 cut(s)	85, 774, 931, 1138, 1241, 1273, 1282, 1388, 1612
FalI	AAGNNNNNCTT	3 cut(s)	29, 1853, 1885
FatI	CATG	9 cut(s)	81, 770, 927, 1134, 1237, 1269, 1278, 1384, 1608
FauI	CCCGC	1 cut(s)	397
FblI	GTMKAC	1 cut(s)	1380
Fnu4HI	GCNGC	7 cut(s)	48, 863, 1203, 1206, 1557, 1886, 2072
FokI	GGATG	6 cut(s)	260, 392, 898, 1031, 1381, 1608
Fsp4HI	GCNGC	7 cut(s)	48, 863, 1203, 1206, 1557, 1886, 2072
FspAI	RTGCGCAY	1 cut(s)	335
FspBI	CTAG	7 cut(s)	294, 531, 551, 1164, 1458, 1523, 2018
FspI	TGCGCA	1 cut(s)	335
GlaI	GCGC	1 cut(s)	335
GluI	GCNGC	7 cut(s)	48, 863, 1203, 1206, 1557, 1886, 2072
HaeIII	GGCC	3 cut(s)	932, 1000, 1902
HgaI	GACGC	1 cut(s)	1883
HhaI	GCGC	1 cut(s)	336
Hin1I	GRCGYC	1 cut(s)	1894
Hin1II	CATG	9 cut(s)	85, 774, 931, 1138, 1241, 1273, 1282, 1388, 1612
Hin6I	GCGC	1 cut(s)	334
HinP1I	GCGC	1 cut(s)	334
HincII	GTYRAC	1 cut(s)	323
HindII	GTYRAC	1 cut(s)	323
HindIII	AAGCTT	1 cut(s)	6
HinfI	GANTC	9 cut(s)	188, 341, 556, 646, 850, 952, 959, 1057, 1282
HphI	GGTGA	6 cut(s)	125, 1114, 1477, 1649, 1927, 2003
Hpy166II	GTNNAC	6 cut(s)	323, 472, 849, 1381, 1729, 1752
Hpy188I	TCNGA	2 cut(s)	346, 1417
Hpy8I	GTNNAC	6 cut(s)	323, 472, 849, 1381, 1729, 1752
Hpy99I	CGWCG	1 cut(s)	938
HpyAV	CCTTC	8 cut(s)	23, 442, 688, 1081, 1111, 1219, 1711, 2131
HpyCH4III	ACNGT	9 cut(s)	327, 541, 1314, 1429, 1497, 1545, 1801, 1907, 2066
HpyCH4IV	ACGT	6 cut(s)	845, 978, 1142, 1227, 1686, 2115
HpyF10VI	GCNNNNNNNGC	5 cut(s)	78, 604, 1202, 1891, 2014
HpyF3I	CTNAG	8 cut(s)	184, 246, 913, 1040, 1061, 1233, 1847, 2013
HpySE526I	ACGT	6 cut(s)	845, 978, 1142, 1227, 1686, 2115
Hsp92I	GRCGYC	1 cut(s)	1894
Hsp92II	CATG	9 cut(s)	85, 774, 931, 1138, 1241, 1273, 1282, 1388, 1612
HspAI	GCGC	1 cut(s)	334
Kzo9I	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
LmnI	GCTCC	2 cut(s)	202, 1322
Lsp1109I	GCAGC	6 cut(s)	849, 1189, 1192, 1568, 1897, 2058
LweI	GCATC	4 cut(s)	321, 926, 1009, 2098
MaeI	CTAG	7 cut(s)	294, 531, 551, 1164, 1458, 1523, 2018
MaeII	ACGT	6 cut(s)	845, 978, 1142, 1227, 1686, 2115
MaeIII	GTNAC	6 cut(s)	26, 628, 1090, 1241, 1615, 1964
MalI	GATC	8 cut(s)	97, 259, 837, 1036, 1356, 1641, 1670, 1838
MboI	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
MflI	RGATCY	2 cut(s)	257, 1354
MhlI	GDGCHC	1 cut(s)	1754
MlyI	GAGTC	4 cut(s)	640, 844, 1066, 1276
MmeI	TCCRAC	1 cut(s)	1346
MseI	TTAA	8 cut(s)	23, 62, 453, 561, 906, 1344, 1691, 1943
MslI	CAYNNNNRTG	2 cut(s)	1484, 1613
MspCI	CTTAAG	1 cut(s)	560
MspR9I	CCNGG	1 cut(s)	1899
Mva1269I	GAATGC	1 cut(s)	627
MvaI	CCWGG	1 cut(s)	1899
MvnI	CGCG	1 cut(s)	506
MwoI	GCNNNNNNNGC	5 cut(s)	78, 604, 1202, 1891, 2014
NcoI	CCATGG	1 cut(s)	1134
NdeII	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
NheI	GCTAGC	1 cut(s)	530
NlaIII	CATG	9 cut(s)	85, 774, 931, 1138, 1241, 1273, 1282, 1388, 1612
NlaIV	GGNNCC	4 cut(s)	259, 385, 874, 999
NmuCI	GTSAC	4 cut(s)	26, 1090, 1615, 1964
NsbI	TGCGCA	1 cut(s)	335
NspI	RCATGY	1 cut(s)	85
NspV	TTCGAA	2 cut(s)	176, 1222
PagI	TCATGA	2 cut(s)	1269, 1278
PcsI	WCGNNNNNNNCGW	1 cut(s)	1001
PctI	GAATGC	1 cut(s)	627
PfeI	GAWTC	5 cut(s)	188, 341, 556, 952, 959
PflMI	CCANNNNNTGG	1 cut(s)	1909
PkrI	GCNGC	7 cut(s)	49, 864, 1204, 1207, 1558, 1887, 2073
PleI	GAGTC	4 cut(s)	640, 844, 1065, 1276
PpsI	GAGTC	4 cut(s)	640, 844, 1065, 1276
Ppu21I	YACGTR	1 cut(s)	846
PpuMI	RGGWCCY	1 cut(s)	1264
Psp1406I	AACGTT	2 cut(s)	1227, 2115
Psp5II	RGGWCCY	1 cut(s)	1264
Psp6I	CCWGG	1 cut(s)	1897
PspGI	CCWGG	1 cut(s)	1897
PspN4I	GGNNCC	4 cut(s)	259, 385, 874, 999
PspPI	GGNCC	2 cut(s)	998, 1264
PspPPI	RGGWCCY	1 cut(s)	1264
PsrI	GAACNNNNNNTAC	2 cut(s)	117, 149
PsuI	RGATCY	2 cut(s)	257, 1354
RsaI	GTAC	6 cut(s)	977, 1171, 1250, 1516, 1730, 1868
RsaNI	GTAC	6 cut(s)	976, 1170, 1249, 1515, 1729, 1867
RseI	CAYNNNNRTG	2 cut(s)	1484, 1613
SaqAI	TTAA	8 cut(s)	23, 62, 453, 561, 906, 1344, 1691, 1943
SatI	GCNGC	7 cut(s)	48, 863, 1203, 1206, 1557, 1886, 2072
Sau3AI	GATC	8 cut(s)	95, 257, 835, 1034, 1354, 1639, 1668, 1836
Sau96I	GGNCC	2 cut(s)	998, 1264
ScaI	AGTACT	1 cut(s)	1868
SchI	GAGTC	4 cut(s)	640, 844, 1066, 1276
ScrFI	CCNGG	1 cut(s)	1899
SduI	GDGCHC	1 cut(s)	1754
SfaNI	GCATC	4 cut(s)	321, 926, 1009, 2098
SfuI	TTCGAA	2 cut(s)	176, 1222
SinI	GGWCC	1 cut(s)	1264
SmiMI	CAYNNNNRTG	2 cut(s)	1484, 1613
SmlI	CTYRAG	1 cut(s)	560
SmoI	CTYRAG	1 cut(s)	560
SsiI	CCGC	3 cut(s)	48, 390, 1883
SspI	AATATT	2 cut(s)	1218, 1960
SspMI	CTAG	7 cut(s)	294, 531, 551, 1164, 1458, 1523, 2018
StyD4I	CCNGG	1 cut(s)	1897
StyI	CCWWGG	1 cut(s)	1134
TaaI	ACNGT	9 cut(s)	327, 541, 1314, 1429, 1497, 1545, 1801, 1907, 2066
TaiI	ACGT	6 cut(s)	848, 981, 1145, 1230, 1689, 2118
TatI	WGTACW	4 cut(s)	1248, 1514, 1728, 1866
TauI	GCSGC	1 cut(s)	50
TfiI	GAWTC	5 cut(s)	188, 341, 556, 952, 959
Tru1I	TTAA	8 cut(s)	23, 62, 453, 561, 906, 1344, 1691, 1943
Tru9I	TTAA	8 cut(s)	23, 62, 453, 561, 906, 1344, 1691, 1943
TscAI	CASTG	5 cut(s)	1099, 1317, 1806, 1833, 1912
TseFI	GTSAC	4 cut(s)	26, 1090, 1615, 1964
TseI	GCWGC	6 cut(s)	862, 1202, 1205, 1556, 1885, 2071
Tsp45I	GTSAC	4 cut(s)	26, 1090, 1615, 1964
TspGWI	ACGGA	3 cut(s)	993, 1335, 1653
TspRI	CASTG	5 cut(s)	1099, 1317, 1806, 1833, 1912
Van91I	CCANNNNNTGG	1 cut(s)	1909
Vha464I	CTTAAG	1 cut(s)	560
VneI	GTGCAC	1 cut(s)	1750
VpaK11BI	GGWCC	1 cut(s)	1264
XapI	RAATTY	6 cut(s)	788, 923, 1105, 1447, 1738, 1805
XceI	RCATGY	1 cut(s)	85
XcmI	CCANNNNNNNNNTGG	2 cut(s)	829, 1141
XmiI	GTMKAC	1 cut(s)	1380
XspI	CTAG	7 cut(s)	294, 531, 551, 1164, 1458, 1523, 2018
ZrmI	AGTACT	1 cut(s)	1868

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.