Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Reverse (-)

54083321 .. 54085778

Gene Structure 2458 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030050

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGGGTTCATTCAATAGGGCCACTAATAATTCCCCTAAACCTGATTTTAAAACTGTCTCCATCAGCTCTCCATGGAACCCAGTAATGGCGTCTCCAGGCAGGGCCAAAGCCAAAGAGGAGGACGAAAAGTTTTACAAGGTGATCAGGGTATTGATTGAGTGGACTGTTTTCCTGTGCATTTTGGGTGTTTTAGTGGCTAGCTTGACTGTCGAGAAACTCGAGAAGCTTAAGGTTTGGGGGCTGGAGTTTTGGAAATGGTGTGTGCTTCTGATGGTAGTATTCGGTGGCACGCTGCTCACATATTGGTTCACGCATTTCATAGTCCTTTTCACCAAGAGGAACATACTGCTCAGGAAAAAGCTGTTGTACTATGTTTATGGAGTGAAGAAGAGTGTTCAGACACTTTCAGGGCCTCCAGTTATAGAGGAAGCCAAGAGGATCGGGAGATCACCGAGCACTGGCCAACTGAGTTTCCTAAGCACCAGGAAGGTCATTGATGTGGGAAAGCTTCAAAAGATGAAGCAGGTTTCGGCTTCTACCATGGAGGTGTACATTGAGGAGGACGACCTCAGCAGGTTCATGACTAATGAAGACATGGAACTTGTGTTTCGATTGATTGATGTCGGAGAGACGGGAAAAATTGATAAAAGAGCTCTAACAGACTGGGTGGTCAAGGTCTACAATGGTCGCAAAGCATTAGATCATGCTCTAACCGACACCAAAGCAGCTGTAAAGCAATTGAACGAAATTGTGACCGGCATCCTTATTGTTATTATCATTGTTGTGTGGCTTATATTAATGGAAGTTGCAACCACGAAAGTACTTGTTTTCATCTCATCACAGCTCGTATTGGCAGCCTTTATCTTTGGAAACACTTGCAAGAACATATTTGAAGCTATCGTATTTGTCTTTGTAATACATCCATTTGATGTTGGTGACCGCTGTCTTGTTGATGGCGTTCCGCTGAGGGTTGAGGAGATGAACATCCTTACTACAGTTTTCTTGAAACTAAATAATGAGAGGGTATATTATCCAAACTCAGTTTTATCAACAAAGACCATTAGCAATTATTATAGAAGTTCAGACATGGGAGATACCGTGGATTTCTCAATCGATGTAATTCCAGTTGACAGGATTAACCTGCTGAAAGCGAAAATAAAGAGTTATTTGGAGAGTAACCCGCAGTATTGGCACCCAGATCACAATGTGGTGGTGATTGAGATTGAAAATGTGAATAAGTTAAAGATGGCACTCTATTTTAATCACACTATGAACTTTCAAGAATTTAGGGAAATAAATAAGCGGAGATCTGAGCTGGTGATTGAATTGATGAGAATATTTGAAGAGCTAAACATCGTATACAATCTGCTTCCTCACGATGTCAGCGTGGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

465

Amino Acids

53.3

Weight (kDa)

8.85

Isoelectric Point (pI)

39.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	293 - 358	6.1e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	668
Acc36I	ACCTGC	3 cut(s)	514, 564, 1149
AccB1I	GGYRCC	1 cut(s)	1191
AccI	GTMKAC	2 cut(s)	678, 1359
AciI	CCGC	4 cut(s)	940, 962, 1181, 1303
AclWI	GGATC	1 cut(s)	446
AcoI	YGGCCR	1 cut(s)	460
AcsI	RAATTY	1 cut(s)	1283
AcyI	GRCGYC	1 cut(s)	89
AdeI	CACNNNGTG	1 cut(s)	1207
AfaI	GTAC	3 cut(s)	368, 551, 822
AfiI	CCNNNNNNNGG	2 cut(s)	85, 458
AflII	CTTAAG	1 cut(s)	227
AgsI	TTSAA	9 cut(s)	13, 512, 742, 893, 1006, 1226, 1280, 1325, 1343
AjnI	CCWGG	2 cut(s)	94, 482
Alw21I	GWGCWC	2 cut(s)	458, 655
Alw26I	GTCTC	3 cut(s)	61, 96, 623
AlwI	GGATC	1 cut(s)	446
Ama87I	CYCGRG	1 cut(s)	218
AoxI	GGCC	5 cut(s)	18, 102, 410, 460, 1389
ApeKI	GCWGC	3 cut(s)	292, 725, 854
ApoI	RAATTY	1 cut(s)	1283
AseI	ATTAAT	1 cut(s)	797
AspS9I	GGNCC	3 cut(s)	18, 102, 410
AsuHPI	GGTGA	6 cut(s)	151, 322, 441, 947, 1225, 1330
AsuNHI	GCTAGC	1 cut(s)	197
AvaI	CYCGRG	1 cut(s)	218
BalI	TGGCCA	1 cut(s)	462
BanI	GGYRCC	1 cut(s)	1191
BanII	GRGCYC	1 cut(s)	655
BbsI	GAAGAC	1 cut(s)	597
Bbv12I	GWGCWC	2 cut(s)	458, 655
BbvCI	CCTCAGC	2 cut(s)	569, 965
BbvI	GCAGC	3 cut(s)	279, 737, 866
BccI	CCATC	4 cut(s)	68, 265, 947, 1240
BciT130I	CCWGG	2 cut(s)	96, 484
BclI	TGATCA	1 cut(s)	141
BcoDI	GTCTC	3 cut(s)	61, 96, 623
BfaI	CTAG	1 cut(s)	198
BfmI	CTRYAG	1 cut(s)	993
BfrI	CTTAAG	1 cut(s)	227
BfuAI	ACCTGC	3 cut(s)	514, 564, 1149
BglII	AGATCT	1 cut(s)	1307
BisI	GCNGC	3 cut(s)	293, 726, 855
BlsI	GCNGC	3 cut(s)	294, 727, 856
BmcAI	AGTACT	1 cut(s)	822
Bme1390I	CCNGG	2 cut(s)	96, 484
BmeT110I	CYCGRG	1 cut(s)	218
BmgT120I	GGNCC	3 cut(s)	18, 102, 410
BmiI	GGNNCC	2 cut(s)	77, 1193
BmrFI	CCNGG	2 cut(s)	96, 484
BmrI	ACTGGG	2 cut(s)	74, 673
BmsI	GCATC	1 cut(s)	768
BmtI	GCTAGC	1 cut(s)	201
BmuI	ACTGGG	2 cut(s)	74, 673
BoxI	GACNNNNGTC	1 cut(s)	942
BpiI	GAAGAC	1 cut(s)	597
BpmI	CTGGAG	3 cut(s)	78, 263, 399
Bpu10I	CCTNAGC	4 cut(s)	350, 476, 569, 965
Bsa29I	ATCGAT	1 cut(s)	1113
BsaBI	GATNNNNATC	1 cut(s)	983
BsaHI	GRCGYC	1 cut(s)	89
BsaJI	CCNNGG	3 cut(s)	71, 540, 1098
BsaXI	ACNNNNNCTCC	2 cut(s)	1083, 1113
Bsc4I	CCNNNNNNNGG	2 cut(s)	85, 458
Bse118I	RCCGGY	1 cut(s)	755
Bse1I	ACTGG	5 cut(s)	80, 416, 463, 668, 1124
Bse8I	GATNNNNATC	1 cut(s)	983
BseBI	CCWGG	2 cut(s)	96, 484
BseCI	ATCGAT	1 cut(s)	1113
BseDI	CCNNGG	3 cut(s)	71, 540, 1098
BseGI	GGATG	3 cut(s)	759, 919, 984
BseJI	GATNNNNATC	1 cut(s)	983
BseLI	CCNNNNNNNGG	2 cut(s)	85, 458
BseMII	CTCAG	6 cut(s)	364, 458, 583, 956, 1053, 1302
BseNI	ACTGG	5 cut(s)	80, 416, 463, 668, 1124
BseRI	GAGGAG	3 cut(s)	131, 572, 989
BseXI	GCAGC	3 cut(s)	279, 737, 866
BshFI	GGCC	5 cut(s)	20, 104, 412, 462, 1391
BshNI	GGYRCC	1 cut(s)	1191
BshVI	ATCGAT	1 cut(s)	1113
BsiHKAI	GWGCWC	2 cut(s)	458, 655
BsiHKCI	CYCGRG	1 cut(s)	218
BsiSI	CCGG	1 cut(s)	756
BslI	CCNNNNNNNGG	2 cut(s)	85, 458
BsmAI	GTCTC	3 cut(s)	61, 96, 623
BsmBI	CGTCTC	2 cut(s)	96, 623
BsnI	GGCC	5 cut(s)	20, 104, 412, 462, 1391
BsoBI	CYCGRG	1 cut(s)	218
Bsp1286I	GDGCHC	2 cut(s)	458, 655
Bsp1407I	TGTACA	1 cut(s)	549
Bsp143I	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
Bsp19I	CCATGG	2 cut(s)	71, 540
BspACI	CCGC	4 cut(s)	940, 962, 1181, 1303
BspANI	GGCC	5 cut(s)	20, 104, 412, 462, 1391
BspCNI	CTCAG	6 cut(s)	363, 459, 582, 957, 1052, 1303
BspDI	ATCGAT	1 cut(s)	1113
BspHI	TCATGA	1 cut(s)	579
BspLI	GGNNCC	2 cut(s)	77, 1193
BspMI	ACCTGC	3 cut(s)	514, 564, 1149
BspOI	GCTAGC	1 cut(s)	201
BspPI	GGATC	1 cut(s)	446
BspQI	GCTCTTC	1 cut(s)	1338
BspT107I	GGYRCC	1 cut(s)	1191
BspTI	CTTAAG	1 cut(s)	227
BsrFI	RCCGGY	1 cut(s)	755
BsrGI	TGTACA	1 cut(s)	549
BsrI	ACTGG	5 cut(s)	80, 416, 463, 668, 1124
BssAI	RCCGGY	1 cut(s)	755
BssECI	CCNNGG	3 cut(s)	71, 540, 1098
BssMI	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
BssNAI	GTATAC	1 cut(s)	1360
BssNI	GRCGYC	1 cut(s)	89
BssT1I	CCWWGG	2 cut(s)	71, 540
Bst1107I	GTATAC	1 cut(s)	1360
Bst2UI	CCWGG	2 cut(s)	96, 484
Bst4CI	ACNGT	5 cut(s)	55, 166, 208, 997, 1099
Bst6I	CTCTTC	2 cut(s)	383, 1338
BstACI	GRCGYC	1 cut(s)	89
BstAFI	CTTAAG	1 cut(s)	227
BstAUI	TGTACA	1 cut(s)	549
BstC8I	GCNNGC	2 cut(s)	199, 290
BstDEI	CTNAG	7 cut(s)	350, 467, 476, 569, 965, 1039, 1311
BstDSI	CCRYGG	3 cut(s)	71, 540, 1098
BstEII	GGTNACC	1 cut(s)	935
BstF5I	GGATG	3 cut(s)	759, 919, 984
BstKTI	GATC	6 cut(s)	144, 441, 449, 703, 1201, 1310
BstMAI	GTCTC	3 cut(s)	61, 96, 623
BstMBI	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
BstMWI	GCNNNNNNNGC	1 cut(s)	1189
BstNI	CCWGG	2 cut(s)	96, 484
BstPAI	GACNNNNGTC	1 cut(s)	942
BstPI	GGTNACC	1 cut(s)	935
BstSCI	CCNGG	2 cut(s)	94, 482
BstSFI	CTRYAG	1 cut(s)	993
BstV1I	GCAGC	3 cut(s)	279, 737, 866
BstV2I	GAAGAC	1 cut(s)	597
BstX2I	RGATCY	1 cut(s)	1307
BstYI	RGATCY	1 cut(s)	1307
BstZ17I	GTATAC	1 cut(s)	1360
Bsu15I	ATCGAT	1 cut(s)	1113
BsuRI	GGCC	5 cut(s)	20, 104, 412, 462, 1391
BsuTUI	ATCGAT	1 cut(s)	1113
BtgI	CCRYGG	3 cut(s)	71, 540, 1098
BtsCI	GGATG	3 cut(s)	759, 919, 984
BtsIMutI	CAGTG	1 cut(s)	456
BveI	ACCTGC	3 cut(s)	514, 564, 1149
Cac8I	GCNNGC	2 cut(s)	199, 290
CciI	TCATGA	1 cut(s)	579
Cfr10I	RCCGGY	1 cut(s)	755
Cfr13I	GGNCC	3 cut(s)	18, 102, 410
ClaI	ATCGAT	1 cut(s)	1113
CseI	GACGC	1 cut(s)	78
Csp6I	GTAC	3 cut(s)	367, 550, 821
CviAII	CATG	6 cut(s)	72, 541, 580, 595, 704, 1087
CviQI	GTAC	3 cut(s)	367, 550, 821
DdeI	CTNAG	7 cut(s)	350, 467, 476, 569, 965, 1039, 1311
DpnI	GATC	6 cut(s)	143, 440, 448, 702, 1200, 1309
DpnII	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
DraI	TTTAAA	1 cut(s)	49
DraIII	CACNNNGTG	1 cut(s)	1207
DrdI	GACNNNNNNGTC	1 cut(s)	668
DseDI	GACNNNNNNGTC	1 cut(s)	668
EaeI	YGGCCR	1 cut(s)	460
Eam1104I	CTCTTC	2 cut(s)	383, 1338
EarI	CTCTTC	2 cut(s)	383, 1338
Ecl136II	GAGCTC	1 cut(s)	653
Eco130I	CCWWGG	2 cut(s)	71, 540
Eco24I	GRGCYC	1 cut(s)	655
Eco53kI	GAGCTC	1 cut(s)	653
Eco88I	CYCGRG	1 cut(s)	218
Eco91I	GGTNACC	1 cut(s)	935
EcoICRI	GAGCTC	1 cut(s)	653
EcoO109I	RGGNCCY	1 cut(s)	410
EcoO65I	GGTNACC	1 cut(s)	935
EcoRII	CCWGG	2 cut(s)	94, 482
EcoT14I	CCWWGG	2 cut(s)	71, 540
EcoT38I	GRGCYC	1 cut(s)	655
ErhI	CCWWGG	2 cut(s)	71, 540
Esp3I	CGTCTC	2 cut(s)	96, 623
FaeI	CATG	6 cut(s)	75, 544, 583, 598, 707, 1090
FatI	CATG	6 cut(s)	71, 540, 579, 594, 703, 1086
FauI	CCCGC	1 cut(s)	1188
FbaI	TGATCA	1 cut(s)	141
FblI	GTMKAC	2 cut(s)	678, 1359
Fnu4HI	GCNGC	3 cut(s)	293, 726, 855
FokI	GGATG	3 cut(s)	746, 906, 971
FriOI	GRGCYC	1 cut(s)	655
Fsp4HI	GCNGC	3 cut(s)	293, 726, 855
FspBI	CTAG	1 cut(s)	198
GluI	GCNGC	3 cut(s)	293, 726, 855
GsuI	CTGGAG	3 cut(s)	78, 263, 399
HaeIII	GGCC	5 cut(s)	20, 104, 412, 462, 1391
HapII	CCGG	1 cut(s)	756
HgaI	GACGC	1 cut(s)	78
Hin1I	GRCGYC	1 cut(s)	89
Hin1II	CATG	6 cut(s)	75, 544, 583, 598, 707, 1090
HincII	GTYRAC	1 cut(s)	1129
HindII	GTYRAC	1 cut(s)	1129
HindIII	AAGCTT	2 cut(s)	224, 506
HpaII	CCGG	1 cut(s)	756
HphI	GGTGA	6 cut(s)	151, 322, 441, 947, 1225, 1330
Hpy166II	GTNNAC	6 cut(s)	162, 309, 550, 679, 1129, 1360
Hpy188I	TCNGA	5 cut(s)	270, 399, 626, 1084, 1312
Hpy188III	TCNNGA	8 cut(s)	211, 220, 352, 442, 580, 1003, 1280, 1376
Hpy8I	GTNNAC	6 cut(s)	162, 309, 550, 679, 1129, 1360
HpyAV	CCTTC	1 cut(s)	481
HpyCH4III	ACNGT	5 cut(s)	55, 166, 208, 997, 1099
HpyCH4V	TGCA	3 cut(s)	177, 809, 879
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1189
HpyF3I	CTNAG	7 cut(s)	350, 467, 476, 569, 965, 1039, 1311
Hsp92I	GRCGYC	1 cut(s)	89
Hsp92II	CATG	6 cut(s)	75, 544, 583, 598, 707, 1090
Ksp22I	TGATCA	1 cut(s)	141
Kzo9I	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
LguI	GCTCTTC	1 cut(s)	1338
Lsp1109I	GCAGC	3 cut(s)	279, 737, 866
LweI	GCATC	1 cut(s)	768
MaeI	CTAG	1 cut(s)	198
MaeIII	GTNAC	3 cut(s)	751, 935, 1175
MalI	GATC	6 cut(s)	143, 440, 448, 702, 1200, 1309
MboI	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
MboII	GAAGA	4 cut(s)	397, 400, 602, 1355
MfeI	CAATTG	1 cut(s)	737
MflI	RGATCY	1 cut(s)	1307
MhlI	GDGCHC	2 cut(s)	458, 655
MlsI	TGGCCA	1 cut(s)	462
MluCI	AATT	8 cut(s)	28, 639, 737, 747, 1066, 1119, 1283, 1325
MluNI	TGGCCA	1 cut(s)	462
MmeI	TCCRAC	1 cut(s)	604
Mox20I	TGGCCA	1 cut(s)	462
MscI	TGGCCA	1 cut(s)	462
MseI	TTAA	6 cut(s)	48, 228, 797, 1137, 1241, 1260
MslI	CAYNNNNRTG	3 cut(s)	497, 545, 782
Msp20I	TGGCCA	1 cut(s)	462
MspA1I	CMGCKG	3 cut(s)	728, 942, 964
MspCI	CTTAAG	1 cut(s)	227
MspI	CCGG	1 cut(s)	756
MspR9I	CCNGG	2 cut(s)	96, 484
MunI	CAATTG	1 cut(s)	737
MvaI	CCWGG	2 cut(s)	96, 484
MwoI	GCNNNNNNNGC	1 cut(s)	1189
NcoI	CCATGG	2 cut(s)	71, 540
NdeII	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
NheI	GCTAGC	1 cut(s)	197
NlaIII	CATG	6 cut(s)	75, 544, 583, 598, 707, 1090
NlaIV	GGNNCC	2 cut(s)	77, 1193
NmuCI	GTSAC	2 cut(s)	751, 935
PaeR7I	CTCGAG	1 cut(s)	218
PagI	TCATGA	1 cut(s)	579
PciSI	GCTCTTC	1 cut(s)	1338
PcsI	WCGNNNNNNNCGW	2 cut(s)	216, 1383
PkrI	GCNGC	3 cut(s)	294, 727, 856
PshAI	GACNNNNGTC	1 cut(s)	942
PshBI	ATTAAT	1 cut(s)	797
Psp124BI	GAGCTC	1 cut(s)	655
Psp6I	CCWGG	2 cut(s)	94, 482
PspEI	GGTNACC	1 cut(s)	935
PspGI	CCWGG	2 cut(s)	94, 482
PspN4I	GGNNCC	2 cut(s)	77, 1193
PspPI	GGNCC	3 cut(s)	18, 102, 410
PsuI	RGATCY	1 cut(s)	1307
PvuII	CAGCTG	1 cut(s)	728
RsaI	GTAC	3 cut(s)	368, 551, 822
RsaNI	GTAC	3 cut(s)	367, 550, 821
RseI	CAYNNNNRTG	3 cut(s)	497, 545, 782
SacI	GAGCTC	1 cut(s)	655
SapI	GCTCTTC	1 cut(s)	1338
SaqAI	TTAA	6 cut(s)	48, 228, 797, 1137, 1241, 1260
SatI	GCNGC	3 cut(s)	293, 726, 855
Sau3AI	GATC	6 cut(s)	141, 438, 446, 700, 1198, 1307
Sau96I	GGNCC	3 cut(s)	18, 102, 410
ScaI	AGTACT	1 cut(s)	822
ScrFI	CCNGG	2 cut(s)	96, 484
SduI	GDGCHC	2 cut(s)	458, 655
SfaNI	GCATC	1 cut(s)	768
SfcI	CTRYAG	1 cut(s)	993
Sfr274I	CTCGAG	1 cut(s)	218
SlaI	CTCGAG	1 cut(s)	218
SmiMI	CAYNNNNRTG	3 cut(s)	497, 545, 782
SmlI	CTYRAG	2 cut(s)	218, 227
SmoI	CTYRAG	2 cut(s)	218, 227
Sse9I	AATT	8 cut(s)	28, 639, 737, 747, 1066, 1119, 1283, 1325
SsiI	CCGC	4 cut(s)	940, 962, 1181, 1303
SspI	AATATT	1 cut(s)	1338
SspMI	CTAG	1 cut(s)	198
SstI	GAGCTC	1 cut(s)	655
StyD4I	CCNGG	2 cut(s)	94, 482
StyI	CCWWGG	2 cut(s)	71, 540
TaaI	ACNGT	5 cut(s)	55, 166, 208, 997, 1099
TaqI	TCGA	4 cut(s)	210, 219, 610, 1113
TasI	AATT	8 cut(s)	28, 639, 737, 747, 1066, 1119, 1283, 1325
TatI	WGTACW	3 cut(s)	366, 549, 820
Tru1I	TTAA	6 cut(s)	48, 228, 797, 1137, 1241, 1260
Tru9I	TTAA	6 cut(s)	48, 228, 797, 1137, 1241, 1260
TscAI	CASTG	1 cut(s)	463
TseFI	GTSAC	2 cut(s)	751, 935
TseI	GCWGC	3 cut(s)	292, 725, 854
Tsp45I	GTSAC	2 cut(s)	751, 935
TspDTI	ATGAA	7 cut(s)	307, 533, 568, 603, 820, 995, 1286
TspRI	CASTG	1 cut(s)	463
Vha464I	CTTAAG	1 cut(s)	227
VspI	ATTAAT	1 cut(s)	797
XapI	RAATTY	1 cut(s)	1283
XhoI	CTCGAG	1 cut(s)	218
XmiI	GTMKAC	2 cut(s)	678, 1359
XspI	CTAG	1 cut(s)	198
ZrmI	AGTACT	1 cut(s)	822

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.