Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000828

Physical Location & Seq

Genomic Coordinates Forward (+)

5543453 .. 5547836

Gene Structure 4384 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1070000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGTCGGAGAAGCTTTCTTCCCATGGAGGTGAAGTTGTACTGGAAGTTCGAAGCGAGGAGAGCAATGCAGCAAAAGGATCTCCCTCAGAAGGCTCAGAATATTCAGCTCCCAGGCAAAGCAAAATTGGCTCACCTGCCAAAGCATCCGCTGAGTCCAGCAGTGGGTTCTCTAAGTCAGTCCCAGTGAGTTACGCTTCGCCTGAATTTTCGAGAGCCAGTCCCGGTAAGCCTCCCAAGATTCCCAACCGCAATGAGAGTCTTGCTAGGAGAAAGTCACTAAATAGGTCAATGTACTCGAAACCAAAGTCAAGATTTGGTGAACCATCAGTAGGCATTGATCACAATATGTTTGAAGAAGATCAAGTTGGTGCAAATTCCCCTTATAGGGTTTCATTCAATAGGGCTTCCCCAATTGATAAATCAGGTATTAGGAGTGTTTCAATCGCAGGGAGTGGGAGGATACCGCCTTCTCCAGGCAGGATTAAAGAGCAGGAGGATGAAGAGATTTACAAGAAGGTTAAATTGAGTAGAGATAATAATAGGAAATTGAAAATGAAAACTGTGGTCTTGCTTGAGTGGGTTGCTTTTCTGGTACTTCTGGCTTGTTTAGTGTCTAGCTTGACTGTCGAGAAATTGGAGAATTATATGCTTTGGGGCTTGGAAATATGGAAATGGTGTGTGCTTGTGATGGTGATTTTCTGTGGAATGTTGGTTACGAATTGGTTTATGCATTTCATAGTGTTTGTGATCGAGAGGAATTTTTTGCTGAGGAAGAAGGTTTTGTATTTTGTTCATGGCATGAAAAAGAGCGTTCAGGTTTCGATTTGGTTGTCTCTGGTTCTTCTCACATGGCTACTGGTGTTCAATCGTGGGGTTGAGCGATCAGAGACTTCCACGAAGATTTTGAATTATGTCACATGGACTCTTGTTTCCATTCTTGTTGGGGCTATTTTGTGGTTGTTAAAAACTTTGTTCCTTAAGATTTTAGCATCTACCTTCCATGTCAACACCTTTTTCGATAGAATCCAAGAATCGATATTCCATCAGTATGTTCTTCAGACACTTTCAGGGCCGGCACTTATAGAGGAGGCTGAGAGGGTTGGGATATGTCCGAGCACTGGGCATCTGAGTTTCAGGACAACAAAGAAGGCTAAAGCTGGGAAGGAGAAAGGGGTGATTGATATGGCAAAGCTTCAGAAGATTAAGCAAGAGAAGGTTTCAGCTTGGACCATGAAGGTATTGGTGGATGCAGTTTCGAGTTCAGGGCTGTCCACCATCTCCCATACATTGGATGAGATGGAAGGGGAAGGAGCTGAACAGATGGATACAGAGATTACTAATGAGATGCAAGCAACTGCCGCTGCCTATCACATTTTTTTGAACGTCGCACCACAGGGTTCCACGTACATTGAGGAGGAGGACCTCATGAGGTTCATGATTAAGGAAGAGGTGGATTATGTGTTTCCGCTGTTTGATGGAGCAGAGACCAGACGTATTGACAGAAAATCTCTAACAGATTGGGTGGTAAAGGTCTACAATGGTCGCAAAGCATTGGCTCATTCTTTAAACGACACCAAAACAGCTGTAAGGCAGTTGAACAAACTTGTGACTGGCATCCTGGTCGTTATTACCATTGTAGTGTGGCTTCTATTGATGGAAATTGCGACCACAAAAGTACTTGTCCTCCTCTCGTCACAGCTCGTAGTTGCAGCTTTTATGTTTGGAAACACTTGCAAGAATATATTTGAAGCTATAATTTTTGTGTTTGTAATGCATCCATTTGATGTTGGTGACCGCTGTGTTGTTGATGGTGTTCCGTTGATGGTTGAGGAGATGAACATCTTAAATACAGTCTTCTTGAAACTCAATATGGAGAAGGTATACTATCCGAATTCAGTTTTGTCCACGAAACCCATCAGCAATTACTTCAGAAGTCCAGACATGGGTGACGTTGTCGAATTCCAAATTGCTTTTTCGACAACAGTGGAGAGGATAGGCCTGCTGAAAGAGAAAATAAAGAAGCATTTGGAGAGGAACTCGCAGCATTGGCATCCTAACCACAAAGTGGTAGTGATTGAAATCGAAAATCTCAACAAGTTAAAGATGGGCCTGTATGTCACTCACACGATGAACTTTCAAGACTTCGGAGAAAAGAATACGCGGAGAACTGAACTAGTCATCGAACTGAAGAAAATGTTGGAAGAGCTCCAAATCAAGTACAATCTGCCGCCCCAAGAAGTTCAACTCACTCAAACTAAATCAGATGCAGCTGTTGCCAACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

762

Amino Acids

86.06

Weight (kDa)

9.09

Isoelectric Point (pI)

44.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	577 - 642	1.2e-07	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12080 AT5G12080 AT5G12080 AT5G19520 AT5G19520
fragaria_vesca	FvH4_2g31211 FvH4_2g31220 FvH4_2g31230 FvH4_2g31240
malus_domestica	MD08G1070000.v1.1 MD15G1052100.v1.1
prunus_persica	Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1 Prupe.1G406100_v2.0.a1
pyrus_communis	pycom08g05800 pycom15g04990 pycom16g12570
rosa_chinensis	RchiOBHm_Chr1g0326931 RchiOBHm_Chr1g0326941 RchiOBHm_Chr6g0276141 RchiOBHm_Chr6g0283901 RchiOBHm_Chr6g0311561 RchiOBHm_Chr6g0311571 RchiOBHm_Chr6g0311581 RchiOBHm_Chr6g0311591
rosa_laevigata	RLG00000010389 RLG00000010390 RLG00000010391 RLG00000010393 RLG00000012788 RLG00000030050
rosa_multiflora	Rmu_sc0003315.1_g000003 Rmu_sc0006781.1_g000009 Rmu_sc0007556.1_g000006 Rmu_sc0018059.1_g000002 Rmu_sc0018059.1_g000003
rosa_roxburghii	Rroxscaffold_4G00323490 Rroxscaffold_7G00157470 Rroxscaffold_7G00157480 Rroxscaffold_7G00157490 Rroxscaffold_7G00185080
rosa_rugosa	Rorug01G0062100 Rorug01G0062300 Rorug06G0158200 Rorug06G0158300 Rorug06G0389800.1 Rorug06G0389900
rosa_samantha	Rh1AG079400 Rh1BG063600 Rh1CG077000 Rh1DG083500 Rh6AG504700 Rh6AG504800 Rh6AG504900 Rh6BG272400 Rh6BG515600 Rh6BG515700 Rh6BG515800 Rh6CG521400 Rh6CG521600 Rh6DG266500 Rh6DG507300 Rh6DG507400
rosa_wichuraiana	Rw1G006300 Rw6G018310 Rw6G023400 Rw6G043960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	142
Acc36I	ACCTGC	1 cut(s)	142
AccB7I	CCANNNNNTGG	2 cut(s)	1288, 2065
AccI	GTMKAC	2 cut(s)	1533, 1881
AccII	CGCG	1 cut(s)	2161
AciI	CCGC	8 cut(s)	147, 247, 464, 1359, 1466, 1795, 2161, 2228
AclWI	GGATC	1 cut(s)	85
AcsI	RAATTY	5 cut(s)	203, 373, 757, 1891, 1958
AcuI	CTGAAG	4 cut(s)	1040, 1178, 1912, 2207
AdeI	CACNNNGTG	1 cut(s)	2065
AfaI	GTAC	6 cut(s)	39, 293, 594, 1406, 1677, 2219
AflII	CTTAAG	1 cut(s)	977
AhlI	ACTAGT	1 cut(s)	2173
AjnI	CCWGG	3 cut(s)	110, 472, 1617
AjuI	GAANNNNNNNTTGG	4 cut(s)	348, 380, 2180, 2212
Alw21I	GWGCWC	2 cut(s)	1118, 2208
Alw26I	GTCTC	3 cut(s)	837, 881, 1478
AlwI	GGATC	1 cut(s)	85
AoxI	GGCC	3 cut(s)	1070, 1996, 2107
ApeKI	GCWGC	5 cut(s)	68, 1361, 1709, 2041, 2267
ApoI	RAATTY	5 cut(s)	203, 373, 757, 1891, 1958
ArsI	GACNNNNNNTTYG	2 cut(s)	290, 322
AspS9I	GGNCC	4 cut(s)	1070, 1227, 1420, 2107
AsuC2I	CCSGG	1 cut(s)	222
AsuHPI	GGTGA	7 cut(s)	41, 123, 329, 703, 1186, 1802, 1958
AsuII	TTCGAA	1 cut(s)	49
AvaII	GGWCC	2 cut(s)	1227, 1420
BanII	GRGCYC	1 cut(s)	2208
BarI	GAAGNNNNNNTAC	2 cut(s)	1629, 1661
BbsI	GAAGAC	1 cut(s)	1846
Bbv12I	GWGCWC	2 cut(s)	1118, 2208
BbvCI	CCTCAGC	1 cut(s)	767
BbvI	GCAGC	5 cut(s)	80, 1348, 1721, 2053, 2279
BcgI	CGANNNNNNTGC	2 cut(s)	1603, 1637
BciT130I	CCWGG	3 cut(s)	112, 474, 1619
BciVI	GTATCC	2 cut(s)	453, 1318
BclI	TGATCA	1 cut(s)	337
BcnI	CCSGG	1 cut(s)	222
BcoDI	GTCTC	3 cut(s)	837, 881, 1478
BcuI	ACTAGT	1 cut(s)	2173
BfaI	CTAG	3 cut(s)	264, 615, 2174
BfrI	CTTAAG	1 cut(s)	977
BfuAI	ACCTGC	1 cut(s)	142
BfuI	GTATCC	2 cut(s)	453, 1318
BisI	GCNGC	7 cut(s)	69, 1359, 1362, 1710, 2042, 2228, 2268
BlsI	GCNGC	7 cut(s)	70, 1360, 1363, 1711, 2043, 2229, 2269
BmcAI	AGTACT	1 cut(s)	1677
Bme1390I	CCNGG	4 cut(s)	112, 222, 474, 1619
Bme18I	GGWCC	2 cut(s)	1227, 1420
BmgT120I	GGNCC	4 cut(s)	1070, 1227, 1420, 2107
BmiI	GGNNCC	1 cut(s)	1399
BmrFI	CCNGG	4 cut(s)	112, 222, 474, 1619
BmrI	ACTGGG	2 cut(s)	176, 1128
BmsI	GCATC	9 cut(s)	152, 998, 1132, 1237, 1335, 1623, 1783, 2059, 2254
BmuI	ACTGGG	2 cut(s)	176, 1128
BpiI	GAAGAC	1 cut(s)	1846
BplI	GAGNNNNNCTC	2 cut(s)	2021, 2053
BpmI	CTGGAG	1 cut(s)	456
Bpu10I	CCTNAGC	1 cut(s)	767
Bpu14I	TTCGAA	1 cut(s)	49
BpuEI	CTTGAG	1 cut(s)	593
BpuMI	CCSGG	1 cut(s)	222
Bsa29I	ATCGAT	1 cut(s)	1034
BsaAI	YACGTR	1 cut(s)	1404
BsaBI	GATNNNNATC	2 cut(s)	1838, 2078
BsaI	GGTCTC	1 cut(s)	1478
BsaJI	CCNNGG	2 cut(s)	22, 110
BsaXI	ACNNNNNCTCC	4 cut(s)	18, 48, 1668, 1698
Bse118I	RCCGGY	1 cut(s)	1072
Bse1I	ACTGG	6 cut(s)	45, 182, 216, 861, 1123, 1615
Bse3DI	GCAATG	2 cut(s)	70, 256
Bse8I	GATNNNNATC	2 cut(s)	1838, 2078
BseBI	CCWGG	3 cut(s)	112, 474, 1619
BseCI	ATCGAT	1 cut(s)	1034
BseDI	CCNNGG	2 cut(s)	22, 110
BseGI	GGATG	7 cut(s)	143, 502, 1252, 1297, 1614, 1774, 2050
BseJI	GATNNNNATC	2 cut(s)	1838, 2078
BseMI	GCAATG	2 cut(s)	70, 256
BseMII	CTCAG	6 cut(s)	99, 108, 141, 758, 1083, 1118
BseNI	ACTGG	6 cut(s)	45, 182, 216, 861, 1123, 1615
BseRI	GAGGAG	6 cut(s)	71, 1100, 1427, 1430, 1676, 1844
BseXI	GCAGC	5 cut(s)	80, 1348, 1721, 2053, 2279
BseYI	CCCAGC	1 cut(s)	1157
Bsh1236I	CGCG	1 cut(s)	2161
BshFI	GGCC	3 cut(s)	1072, 1998, 2109
BshVI	ATCGAT	1 cut(s)	1034
BsiHKAI	GWGCWC	2 cut(s)	1118, 2208
BsiSI	CCGG	2 cut(s)	222, 1073
BslFI	GGGAC	2 cut(s)	164, 204
BsmAI	GTCTC	3 cut(s)	837, 881, 1478
BsmFI	GGGAC	2 cut(s)	164, 204
BsnI	GGCC	3 cut(s)	1072, 1998, 2109
Bso31I	GGTCTC	1 cut(s)	1478
Bsp119I	TTCGAA	1 cut(s)	49
Bsp1286I	GDGCHC	2 cut(s)	1118, 2208
Bsp143I	GATC	5 cut(s)	77, 337, 358, 747, 881
Bsp19I	CCATGG	1 cut(s)	22
BspACI	CCGC	8 cut(s)	147, 247, 464, 1359, 1466, 1795, 2161, 2228
BspANI	GGCC	3 cut(s)	1072, 1998, 2109
BspCNI	CTCAG	6 cut(s)	98, 107, 142, 759, 1084, 1119
BspDI	ATCGAT	1 cut(s)	1034
BspFNI	CGCG	1 cut(s)	2161
BspHI	TCATGA	2 cut(s)	1425, 1434
BspLI	GGNNCC	1 cut(s)	1399
BspMI	ACCTGC	1 cut(s)	142
BspPI	GGATC	1 cut(s)	85
BspQI	GCTCTTC	1 cut(s)	2196
BspT104I	TTCGAA	1 cut(s)	49
BspTI	CTTAAG	1 cut(s)	977
BspTNI	GGTCTC	1 cut(s)	1478
BsrDI	GCAATG	2 cut(s)	70, 256
BsrFI	RCCGGY	1 cut(s)	1072
BsrI	ACTGG	6 cut(s)	45, 182, 216, 861, 1123, 1615
BssAI	RCCGGY	1 cut(s)	1072
BssECI	CCNNGG	2 cut(s)	22, 110
BssMI	GATC	5 cut(s)	77, 337, 358, 747, 881
BssNAI	GTATAC	1 cut(s)	1882
BssT1I	CCWWGG	1 cut(s)	22
Bst1107I	GTATAC	1 cut(s)	1882
Bst2UI	CCWGG	3 cut(s)	112, 474, 1619
Bst4CI	ACNGT	4 cut(s)	562, 625, 1852, 1984
Bst6I	CTCTTC	3 cut(s)	495, 1440, 2196
BstAFI	CTTAAG	1 cut(s)	977
BstAPI	GCANNNNNTGC	1 cut(s)	2273
BstBAI	YACGTR	1 cut(s)	1404
BstBI	TTCGAA	1 cut(s)	49
BstC8I	GCNNGC	3 cut(s)	1074, 1350, 2000
BstDEI	CTNAG	7 cut(s)	85, 94, 150, 171, 767, 1092, 1127
BstDSI	CCRYGG	1 cut(s)	22
BstEII	GGTNACC	1 cut(s)	1790
BstENI	CCTNNNNNAGG	1 cut(s)	471
BstF5I	GGATG	7 cut(s)	143, 502, 1252, 1297, 1614, 1774, 2050
BstFNI	CGCG	1 cut(s)	2161
BstKTI	GATC	5 cut(s)	80, 340, 361, 750, 884
BstMAI	GTCTC	3 cut(s)	837, 881, 1478
BstMBI	GATC	5 cut(s)	77, 337, 358, 747, 881
BstMWI	GCNNNNNNNGC	5 cut(s)	60, 126, 1358, 2047, 2273
BstNI	CCWGG	3 cut(s)	112, 474, 1619
BstPI	GGTNACC	1 cut(s)	1790
BstSCI	CCNGG	4 cut(s)	110, 220, 472, 1617
BstUI	CGCG	1 cut(s)	2161
BstV1I	GCAGC	5 cut(s)	80, 1348, 1721, 2053, 2279
BstV2I	GAAGAC	1 cut(s)	1846
BstX2I	RGATCY	1 cut(s)	77
BstYI	RGATCY	1 cut(s)	77
BstZ17I	GTATAC	1 cut(s)	1882
Bsu15I	ATCGAT	1 cut(s)	1034
BsuI	GTATCC	2 cut(s)	453, 1318
BsuRI	GGCC	3 cut(s)	1072, 1998, 2109
BsuTUI	ATCGAT	1 cut(s)	1034
BtgI	CCRYGG	1 cut(s)	22
BtsCI	GGATG	7 cut(s)	143, 502, 1252, 1297, 1614, 1774, 2050
BtsI	GCAGTG	1 cut(s)	166
BtsIMutI	CAGTG	4 cut(s)	166, 189, 1116, 1989
BveI	ACCTGC	1 cut(s)	142
Cac8I	GCNNGC	3 cut(s)	1074, 1350, 2000
CciI	TCATGA	2 cut(s)	1425, 1434
Cfr10I	RCCGGY	1 cut(s)	1072
Cfr13I	GGNCC	4 cut(s)	1070, 1227, 1420, 2107
ClaI	ATCGAT	1 cut(s)	1034
Csp6I	GTAC	6 cut(s)	38, 292, 593, 1405, 1676, 2218
CspCI	CAANNNNNGTGG	2 cut(s)	1390, 1425
CviQI	GTAC	6 cut(s)	38, 292, 593, 1405, 1676, 2218
DdeI	CTNAG	7 cut(s)	85, 94, 150, 171, 767, 1092, 1127
DpnI	GATC	5 cut(s)	79, 339, 360, 749, 883
DpnII	GATC	5 cut(s)	77, 337, 358, 747, 881
DraI	TTTAAA	1 cut(s)	1566
DraIII	CACNNNGTG	1 cut(s)	2065
Eam1104I	CTCTTC	3 cut(s)	495, 1440, 2196
EarI	CTCTTC	3 cut(s)	495, 1440, 2196
Ecl136II	GAGCTC	1 cut(s)	2206
Eco130I	CCWWGG	1 cut(s)	22
Eco147I	AGGCCT	1 cut(s)	1998
Eco24I	GRGCYC	1 cut(s)	2208
Eco31I	GGTCTC	1 cut(s)	1478
Eco47I	GGWCC	2 cut(s)	1227, 1420
Eco53kI	GAGCTC	1 cut(s)	2206
Eco57I	CTGAAG	4 cut(s)	1040, 1178, 1912, 2207
Eco91I	GGTNACC	1 cut(s)	1790
EcoICRI	GAGCTC	1 cut(s)	2206
EcoNI	CCTNNNNNAGG	1 cut(s)	471
EcoO109I	RGGNCCY	1 cut(s)	1420
EcoO65I	GGTNACC	1 cut(s)	1790
EcoRI	GAATTC	2 cut(s)	1891, 1958
EcoRII	CCWGG	3 cut(s)	110, 472, 1617
EcoT14I	CCWWGG	1 cut(s)	22
EcoT22I	ATGCAT	2 cut(s)	732, 1776
EcoT38I	GRGCYC	1 cut(s)	2208
ErhI	CCWWGG	1 cut(s)	22
FaqI	GGGAC	2 cut(s)	164, 204
FbaI	TGATCA	1 cut(s)	337
FblI	GTMKAC	2 cut(s)	1533, 1881
Fnu4HI	GCNGC	7 cut(s)	69, 1359, 1362, 1710, 2042, 2228, 2268
FokI	GGATG	7 cut(s)	130, 509, 1259, 1304, 1601, 1761, 2037
FriOI	GRGCYC	1 cut(s)	2208
Fsp4HI	GCNGC	7 cut(s)	69, 1359, 1362, 1710, 2042, 2228, 2268
FspBI	CTAG	3 cut(s)	264, 615, 2174
GluI	GCNGC	7 cut(s)	69, 1359, 1362, 1710, 2042, 2228, 2268
GsaI	CCCAGC	1 cut(s)	1161
GsuI	CTGGAG	1 cut(s)	456
HaeIII	GGCC	3 cut(s)	1072, 1998, 2109
HapII	CCGG	2 cut(s)	222, 1073
HincII	GTYRAC	1 cut(s)	1006
HindII	GTYRAC	1 cut(s)	1006
HindIII	AAGCTT	2 cut(s)	11, 1190
HinfI	GANTC	6 cut(s)	152, 238, 256, 922, 1023, 1031
HpaII	CCGG	2 cut(s)	222, 1073
HphI	GGTGA	7 cut(s)	41, 123, 329, 703, 1186, 1802, 1958
Hpy166II	GTNNAC	6 cut(s)	320, 1006, 1272, 1534, 1882, 1905
Hpy8I	GTNNAC	6 cut(s)	320, 1006, 1272, 1534, 1882, 1905
Hpy99I	CGWCG	1 cut(s)	1388
HpyCH4III	ACNGT	4 cut(s)	562, 625, 1852, 1984
HpyCH4IV	ACGT	4 cut(s)	1383, 1403, 1492, 1950
HpyCH4V	TGCA	9 cut(s)	68, 371, 730, 1250, 1348, 1709, 1734, 1774, 2267
HpyF10VI	GCNNNNNNNGC	5 cut(s)	60, 126, 1358, 2047, 2273
HpyF3I	CTNAG	7 cut(s)	85, 94, 150, 171, 767, 1092, 1127
HpySE526I	ACGT	4 cut(s)	1383, 1403, 1492, 1950
KroI	GCCGGC	1 cut(s)	1072
KroNI	GCCGGC	1 cut(s)	1074
Ksp22I	TGATCA	1 cut(s)	337
Kzo9I	GATC	5 cut(s)	77, 337, 358, 747, 881
LguI	GCTCTTC	1 cut(s)	2196
LmnI	GCTCC	4 cut(s)	112, 1310, 1478, 2211
Lsp1109I	GCAGC	5 cut(s)	80, 1348, 1721, 2053, 2279
LweI	GCATC	9 cut(s)	152, 998, 1132, 1237, 1335, 1623, 1783, 2059, 2254
MaeI	CTAG	3 cut(s)	264, 615, 2174
MaeII	ACGT	4 cut(s)	1383, 1403, 1492, 1950
MaeIII	GTNAC	9 cut(s)	188, 273, 712, 913, 1606, 1692, 1790, 1946, 2116
MalI	GATC	5 cut(s)	79, 339, 360, 749, 883
MboI	GATC	5 cut(s)	77, 337, 358, 747, 881
MfeI	CAATTG	1 cut(s)	411
MflI	RGATCY	1 cut(s)	77
MhlI	GDGCHC	2 cut(s)	1118, 2208
MlyI	GAGTC	3 cut(s)	161, 265, 916
MmeI	TCCRAC	1 cut(s)	2178
Mph1103I	ATGCAT	2 cut(s)	732, 1776
MroNI	GCCGGC	1 cut(s)	1072
MseI	TTAA	9 cut(s)	483, 519, 962, 978, 1203, 1440, 1565, 1844, 2099
MslI	CAYNNNNRTG	3 cut(s)	27, 1047, 1637
MspA1I	CMGCKG	6 cut(s)	149, 1361, 1468, 1583, 1797, 2270
MspCI	CTTAAG	1 cut(s)	977
MspI	CCGG	2 cut(s)	222, 1073
MspR9I	CCNGG	4 cut(s)	112, 222, 474, 1619
MunI	CAATTG	1 cut(s)	411
MvaI	CCWGG	3 cut(s)	112, 474, 1619
MvnI	CGCG	1 cut(s)	2161
MwoI	GCNNNNNNNGC	5 cut(s)	60, 126, 1358, 2047, 2273
NaeI	GCCGGC	1 cut(s)	1074
NciI	CCSGG	1 cut(s)	222
NcoI	CCATGG	1 cut(s)	22
NdeII	GATC	5 cut(s)	77, 337, 358, 747, 881
NgoMIV	GCCGGC	1 cut(s)	1072
NlaIV	GGNNCC	1 cut(s)	1399
NmuCI	GTSAC	7 cut(s)	273, 913, 1606, 1692, 1790, 1946, 2116
NsiI	ATGCAT	2 cut(s)	732, 1776
NspV	TTCGAA	1 cut(s)	49
PagI	TCATGA	2 cut(s)	1425, 1434
PaqCI	CACCTGC	1 cut(s)	142
PceI	AGGCCT	1 cut(s)	1998
PciSI	GCTCTTC	1 cut(s)	2196
PdiI	GCCGGC	1 cut(s)	1074
PfeI	GAWTC	3 cut(s)	238, 1023, 1031
PflFI	GACNNNGTC	1 cut(s)	1952
PflMI	CCANNNNNTGG	2 cut(s)	1288, 2065
PkrI	GCNGC	7 cut(s)	70, 1360, 1363, 1711, 2043, 2229, 2269
PleI	GAGTC	3 cut(s)	160, 264, 916
PpsI	GAGTC	3 cut(s)	160, 264, 916
Ppu21I	YACGTR	1 cut(s)	1404
PpuMI	RGGWCCY	1 cut(s)	1420
Psp124BI	GAGCTC	1 cut(s)	2208
Psp5II	RGGWCCY	1 cut(s)	1420
Psp6I	CCWGG	3 cut(s)	110, 472, 1617
PspEI	GGTNACC	1 cut(s)	1790
PspFI	CCCAGC	1 cut(s)	1157
PspGI	CCWGG	3 cut(s)	110, 472, 1617
PspN4I	GGNNCC	1 cut(s)	1399
PspPI	GGNCC	4 cut(s)	1070, 1227, 1420, 2107
PspPPI	RGGWCCY	1 cut(s)	1420
PsrI	GAACNNNNNNTAC	2 cut(s)	30, 62
PsuI	RGATCY	1 cut(s)	77
PsyI	GACNNNGTC	1 cut(s)	1952
PvuII	CAGCTG	2 cut(s)	1583, 2270
RsaI	GTAC	6 cut(s)	39, 293, 594, 1406, 1677, 2219
RsaNI	GTAC	6 cut(s)	38, 292, 593, 1405, 1676, 2218
RseI	CAYNNNNRTG	3 cut(s)	27, 1047, 1637
SacI	GAGCTC	1 cut(s)	2208
SapI	GCTCTTC	1 cut(s)	2196
SaqAI	TTAA	9 cut(s)	483, 519, 962, 978, 1203, 1440, 1565, 1844, 2099
SatI	GCNGC	7 cut(s)	69, 1359, 1362, 1710, 2042, 2228, 2268
Sau3AI	GATC	5 cut(s)	77, 337, 358, 747, 881
Sau96I	GGNCC	4 cut(s)	1070, 1227, 1420, 2107
ScaI	AGTACT	1 cut(s)	1677
SchI	GAGTC	3 cut(s)	161, 265, 916
ScrFI	CCNGG	4 cut(s)	112, 222, 474, 1619
SduI	GDGCHC	2 cut(s)	1118, 2208
SfaNI	GCATC	9 cut(s)	152, 998, 1132, 1237, 1335, 1623, 1783, 2059, 2254
SfuI	TTCGAA	1 cut(s)	49
SinI	GGWCC	2 cut(s)	1227, 1420
SmiMI	CAYNNNNRTG	3 cut(s)	27, 1047, 1637
SmlI	CTYRAG	2 cut(s)	572, 977
SmoI	CTYRAG	2 cut(s)	572, 977
SpeI	ACTAGT	1 cut(s)	2173
SseBI	AGGCCT	1 cut(s)	1998
SsiI	CCGC	8 cut(s)	147, 247, 464, 1359, 1466, 1795, 2161, 2228
SspI	AATATT	1 cut(s)	101
SspMI	CTAG	3 cut(s)	264, 615, 2174
SstI	GAGCTC	1 cut(s)	2208
StuI	AGGCCT	1 cut(s)	1998
StyD4I	CCNGG	4 cut(s)	110, 220, 472, 1617
StyI	CCWWGG	1 cut(s)	22
TaaI	ACNGT	4 cut(s)	562, 625, 1852, 1984
TaiI	ACGT	4 cut(s)	1386, 1406, 1495, 1953
TatI	WGTACW	4 cut(s)	37, 291, 1675, 2217
TauI	GCSGC	2 cut(s)	1361, 2230
TfiI	GAWTC	3 cut(s)	238, 1023, 1031
Tru1I	TTAA	9 cut(s)	483, 519, 962, 978, 1203, 1440, 1565, 1844, 2099
Tru9I	TTAA	9 cut(s)	483, 519, 962, 978, 1203, 1440, 1565, 1844, 2099
TscAI	CASTG	4 cut(s)	166, 189, 1123, 1989
TseFI	GTSAC	7 cut(s)	273, 913, 1606, 1692, 1790, 1946, 2116
TseI	GCWGC	5 cut(s)	68, 1361, 1709, 2041, 2267
Tsp45I	GTSAC	7 cut(s)	273, 913, 1606, 1692, 1790, 1946, 2116
TspGWI	ACGGA	1 cut(s)	1806
TspRI	CASTG	4 cut(s)	166, 189, 1123, 1989
Tth111I	GACNNNGTC	1 cut(s)	1952
Van91I	CCANNNNNTGG	2 cut(s)	1288, 2065
Vha464I	CTTAAG	1 cut(s)	977
VpaK11BI	GGWCC	2 cut(s)	1227, 1420
XagI	CCTNNNNNAGG	1 cut(s)	471
XapI	RAATTY	5 cut(s)	203, 373, 757, 1891, 1958
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1237
XmiI	GTMKAC	2 cut(s)	1533, 1881
XspI	CTAG	3 cut(s)	264, 615, 2174
ZrmI	AGTACT	1 cut(s)	1677
Zsp2I	ATGCAT	2 cut(s)	732, 1776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.