FvH4_1g04610

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000731
Physical Location & Seq
Reverse (-)
2444902 .. 2449014
4113 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04610.t2

Sequence Viewer

Length: 1566 bp
ATGGCGGACGGTATCGAGGACCCTGCTGCTGACTCCGAAAGTCTGCACGAAACCGAGATCAGCATTGGATCTCATATCGATAAACTTTCAGAAGGGCCAGAAAGCGAGATAAGCTTTACCCAAAAGATGTATAGAAAGAAACTTAAGTGGACATCCGAAATCGAAGAAAAGCTCCACAATGTGCGGCCATGGAACTTGAGCGTCTACAGAGTGCCAAATAGACTTCATCAAGTCAGTGAGGATGCCTACACCCCTCATGTTGTCTCCATTGGTCCTTTCCATAGACACAAGCCTCATCTAGTAGCTATGAGAGAGCATAAGTTGCGTTATGCGCTATACTTTTTTAAGCGAGCACAAGAAACAGCACAGAAACGAGCTCTAAATTTCAGCTTTGGATCACGAGAATCTTCCGAATGCATGAGAGAATGGGCAACTGCTATCTATGATTCGGATGCATTGGTTCGTGCAAGTTACGCTGAAGACATGAGCAACATTAATCAGGAGGAACTAGCAGAAATAATGTTGGTCGATGGTTGCTTCATATTGGAACTTCTCATCAGGTTTTACCTATACATGGACAGGAAGTTAGAAGGCCATGAACCTGATCCAATTCTAGAAAGTGCTTGGATGATCGCAGATATACGTCATGATCTGGCATTGCTTGAAAACCAAATCCCCTTGTGTATTATACTTGATTTGTATGATGCTATTAGTGGTCATCAGACACTTATCGACAATGATTTGCCTCCGCCATATTGTCTTGCTCTTCACTTCTTTCAACCAGTGAGCCTCAAGGGAAGCATAGTAACAGAGCGGAGACATGACTCCCAAGATTACAAGCACTTGCTGGATATCTTGCACAAGTTCTATTTCCTCCCAACCAAAACTACTGACTTGTCTATGAGGCTCAATGTGGATTTGATCCCTGAGGAGCAGCGTCTTAGGGCAATCCAAAAGAATAAAGTATGGGGATTCAATTATAGTGCTTCTGAACTTTTGGAGTCTGGAGTCGAGTTTGACATTGGCTCCAAGCAGGGCCAGTTGCTGGACATAACTTTCAATGAGGGAAAAATCAGAATTCCACCACTGATTATTCAAGAAACAACGAGTTCATTGTTGAGGAACCTGATTGCTTATGAGCAATGCAGTCTCAATAGTACGCAAGCGGTTACATCATATGCCTGCCTCATGAAGAGTCTCATCGCTTCCTCACGAGATAGCAATCTGCTTCTAAGGAAGGGGATAACGAAATATCACCACTGGATTGGAGGTGAAGAGGAATATTTGGCTCAAATATTCGAAGGTATACTAGATGAAGTTGTTGTCAAGGAATTCCATTTTAGAACCTTGTGCGATGAGGTGAATGAATATTGTACCTCCTGGTTTCATCTGAGAAAACTCAAAGTATTTCTGAGGGTGCGGGTTCAAAGATATACAACGCTCCTATTCAGCACTTATTTTGCTTCCCCGTGGACTTTCATATCATTCATGGCCGCTCTTGCCATGCTCATTCTCACATCTTTGCAGACATACTATACCATACGCACAGCTCATACTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

522

Amino Acids

60.45

Weight (kDa)

5.85

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 69 - 506 2.1e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1045
AccBSI CCGCTC 2 cut(s) 814, 1496
AccI GTMKAC 2 cut(s) 204, 1306
AciI CCGC 7 cut(s) 5, 184, 749, 814, 1166, 1420, 1494
AclWI GGATC 4 cut(s) 76, 403, 599, 916
AcoI YGGCCR 2 cut(s) 185, 1491
AcsI RAATTY 3 cut(s) 382, 1077, 1331
AcuI CTGAAG 1 cut(s) 498
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 2 cut(s) 1159, 1375
AfiI CCNNNNNNNGG 2 cut(s) 574, 1045
AflII CTTAAG 1 cut(s) 143
AgsI TTSAA 6 cut(s) 665, 779, 976, 1060, 1097, 1427
AjnI CCWGG 1 cut(s) 1379
AluBI AGCT 6 cut(s) 114, 172, 305, 377, 390, 1550
AluI AGCT 6 cut(s) 114, 172, 305, 377, 390, 1550
Alw21I GWGCWC 2 cut(s) 355, 379
Alw26I GTCTC 4 cut(s) 268, 811, 1154, 1202
AlwI GGATC 4 cut(s) 76, 403, 599, 916
AlwNI CAGNNNCTG 1 cut(s) 1045
AoxI GGCC 5 cut(s) 95, 185, 592, 1036, 1491
ApeKI GCWGC 2 cut(s) 26, 934
ApoI RAATTY 3 cut(s) 382, 1077, 1331
AseI ATTAAT 2 cut(s) 495, 1560
AspLEI GCGC 1 cut(s) 334
AspS9I GGNCC 4 cut(s) 19, 95, 272, 1036
AsuHPI GGTGA 3 cut(s) 1247, 1283, 1372
AsuII TTCGAA 1 cut(s) 1299
AvaII GGWCC 2 cut(s) 19, 272
AxyI CCTNAGG 1 cut(s) 927
BanII GRGCYC 1 cut(s) 379
BauI CACGAG 2 cut(s) 399, 1212
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 2 cut(s) 355, 379
BbvI GCAGC 2 cut(s) 13, 946
BccI CCATC 1 cut(s) 524
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 1381
BcoDI GTCTC 4 cut(s) 268, 811, 1154, 1202
BfaI CTAG 4 cut(s) 299, 509, 614, 1310
BfmI CTRYAG 1 cut(s) 205
BfrI CTTAAG 1 cut(s) 143
BisI GCNGC 4 cut(s) 27, 185, 935, 1494
BlsI GCNGC 4 cut(s) 28, 186, 936, 1495
Bme1390I CCNGG 1 cut(s) 1381
Bme18I GGWCC 2 cut(s) 19, 272
BmgT120I GGNCC 4 cut(s) 19, 95, 272, 1036
BmiI GGNNCC 3 cut(s) 21, 1027, 1124
BmrFI CCNGG 1 cut(s) 1381
BmsI GCATC 3 cut(s) 232, 442, 694
BpiI GAAGAC 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 1026
Bpu14I TTCGAA 1 cut(s) 1299
BpuEI CTTGAG 2 cut(s) 217, 776
Bsa29I ATCGAT 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 1221
BsaJI CCNNGG 2 cut(s) 188, 1469
BsaXI ACNNNNNCTCC 2 cut(s) 1010, 1040
Bsc4I CCNNNNNNNGG 2 cut(s) 574, 1045
Bse1I ACTGG 3 cut(s) 782, 1039, 1265
Bse21I CCTNAGG 1 cut(s) 927
Bse3DI GCAATG 2 cut(s) 656, 1148
Bse8I GATNNNNATC 1 cut(s) 1221
BseBI CCWGG 1 cut(s) 1381
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 188, 1469
BseGI GGATG 4 cut(s) 152, 247, 457, 633
BseJI GATNNNNATC 1 cut(s) 1221
BseLI CCNNNNNNNGG 2 cut(s) 574, 1045
BseMI GCAATG 2 cut(s) 656, 1148
BseMII CTCAG 3 cut(s) 918, 1382, 1403
BseNI ACTGG 3 cut(s) 782, 1039, 1265
BseRI GAGGAG 1 cut(s) 944
BseXI GCAGC 2 cut(s) 13, 946
BsgI GTGCAG 1 cut(s) 29
BshFI GGCC 5 cut(s) 97, 187, 594, 1038, 1493
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 355, 379
BslI CCNNNNNNNGG 2 cut(s) 574, 1045
BsmAI GTCTC 4 cut(s) 268, 811, 1154, 1202
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 5 cut(s) 97, 187, 594, 1038, 1493
Bsp119I TTCGAA 1 cut(s) 1299
Bsp1286I GDGCHC 2 cut(s) 355, 379
Bsp143I GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 7 cut(s) 5, 184, 749, 814, 1166, 1420, 1494
BspANI GGCC 5 cut(s) 97, 187, 594, 1038, 1493
BspCNI CTCAG 3 cut(s) 919, 1383, 1404
BspDI ATCGAT 1 cut(s) 78
BspHI TCATGA 2 cut(s) 646, 1188
BspLI GGNNCC 3 cut(s) 21, 1027, 1124
BspPI GGATC 4 cut(s) 76, 403, 599, 916
BspQI GCTCTTC 1 cut(s) 771
BspT104I TTCGAA 1 cut(s) 1299
BspTI CTTAAG 1 cut(s) 143
BsrBI CCGCTC 2 cut(s) 814, 1496
BsrDI GCAATG 2 cut(s) 656, 1148
BsrI ACTGG 3 cut(s) 782, 1039, 1265
BssECI CCNNGG 2 cut(s) 188, 1469
BssMI GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
BssNAI GTATAC 1 cut(s) 1307
BssSI CACGAG 2 cut(s) 399, 1212
BssT1I CCWWGG 1 cut(s) 188
Bst1107I GTATAC 1 cut(s) 1307
Bst2BI CACGAG 2 cut(s) 399, 1212
Bst2UI CCWGG 1 cut(s) 1381
Bst4CI ACNGT 1 cut(s) 11
Bst6I CTCTTC 3 cut(s) 771, 1187, 1269
BstAFI CTTAAG 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 1299
BstC8I GCNNGC 3 cut(s) 351, 1164, 1183
BstDEI CTNAG 5 cut(s) 927, 941, 1232, 1391, 1412
BstDSI CCRYGG 2 cut(s) 188, 1469
BstF5I GGATG 4 cut(s) 152, 247, 457, 633
BstHHI GCGC 1 cut(s) 334
BstKTI GATC 7 cut(s) 60, 71, 398, 607, 633, 652, 924
BstMAI GTCTC 4 cut(s) 268, 811, 1154, 1202
BstMBI GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
BstMWI GCNNNNNNNGC 5 cut(s) 111, 322, 331, 473, 1499
BstNI CCWGG 1 cut(s) 1381
BstSCI CCNGG 1 cut(s) 1379
BstSFI CTRYAG 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 13, 946
BstV2I GAAGAC 1 cut(s) 486
BstX2I RGATCY 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 1265
BstYI RGATCY 1 cut(s) 68
BstZ17I GTATAC 1 cut(s) 1307
Bsu15I ATCGAT 1 cut(s) 78
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 5 cut(s) 97, 187, 594, 1038, 1493
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 2 cut(s) 188, 1469
BtgZI GCGATG 2 cut(s) 1186, 1368
BtsCI GGATG 4 cut(s) 152, 247, 457, 633
BtsIMutI CAGTG 4 cut(s) 241, 789, 1085, 1258
Cac8I GCNNGC 3 cut(s) 351, 1164, 1183
CaiI CAGNNNCTG 1 cut(s) 1045
CciI TCATGA 2 cut(s) 646, 1188
CfoI GCGC 1 cut(s) 334
Cfr13I GGNCC 4 cut(s) 19, 95, 272, 1036
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 2 cut(s) 190, 926
Csp6I GTAC 2 cut(s) 1158, 1374
CviQI GTAC 2 cut(s) 1158, 1374
DdeI CTNAG 5 cut(s) 927, 941, 1232, 1391, 1412
DpnI GATC 7 cut(s) 59, 70, 397, 606, 632, 651, 923
DpnII GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
DraIII CACNNNGTG 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 185, 1491
Eam1104I CTCTTC 3 cut(s) 771, 1187, 1269
EarI CTCTTC 3 cut(s) 771, 1187, 1269
EciI GGCGGA 2 cut(s) 20, 738
Ecl136II GAGCTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 379
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 2 cut(s) 19, 272
Eco53kI GAGCTC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 498
Eco81I CCTNAGG 1 cut(s) 927
EcoICRI GAGCTC 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 19
EcoRI GAATTC 2 cut(s) 1077, 1331
EcoRII CCWGG 1 cut(s) 1379
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 188
EcoT22I ATGCAT 2 cut(s) 419, 457
EcoT38I GRGCYC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 188
FauI CCCGC 1 cut(s) 1413
FauNDI CATATG 1 cut(s) 1177
FblI GTMKAC 2 cut(s) 204, 1306
Fnu4HI GCNGC 4 cut(s) 27, 185, 935, 1494
FokI GGATG 4 cut(s) 139, 254, 464, 640
FriOI GRGCYC 1 cut(s) 379
Fsp4HI GCNGC 4 cut(s) 27, 185, 935, 1494
FspBI CTAG 4 cut(s) 299, 509, 614, 1310
GlaI GCGC 1 cut(s) 333
GluI GCNGC 4 cut(s) 27, 185, 935, 1494
GsuI CTGGAG 1 cut(s) 1026
HaeIII GGCC 5 cut(s) 97, 187, 594, 1038, 1493
HgaI GACGC 2 cut(s) 190, 926
HhaI GCGC 1 cut(s) 334
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
HindIII AAGCTT 1 cut(s) 112
HinfI GANTC 8 cut(s) 32, 404, 446, 824, 972, 1001, 1008, 1195
HphI GGTGA 3 cut(s) 1247, 1283, 1372
Hpy166II GTNNAC 4 cut(s) 150, 205, 1307, 1473
Hpy188III TCNNGA 8 cut(s) 399, 500, 614, 647, 1005, 1097, 1189, 1212
Hpy8I GTNNAC 4 cut(s) 150, 205, 1307, 1473
HpyAV CCTTC 4 cut(s) 86, 584, 1231, 1295
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 7 cut(s) 46, 417, 455, 467, 859, 1146, 1525
HpyF10VI GCNNNNNNNGC 5 cut(s) 111, 322, 331, 473, 1499
HpyF3I CTNAG 5 cut(s) 927, 941, 1232, 1391, 1412
HpySE526I ACGT 1 cut(s) 643
HspAI GCGC 1 cut(s) 332
Kzo9I GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 4 cut(s) 177, 931, 1031, 1446
Lsp1109I GCAGC 2 cut(s) 13, 946
LweI GCATC 3 cut(s) 232, 442, 694
MaeI CTAG 4 cut(s) 299, 509, 614, 1310
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 3 cut(s) 470, 805, 1168
MalI GATC 7 cut(s) 59, 70, 397, 606, 632, 651, 923
MbiI CCGCTC 2 cut(s) 814, 1496
MboI GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
MboII GAAGA 6 cut(s) 176, 399, 491, 758, 1204, 1286
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 2 cut(s) 355, 379
MluCI AATT 6 cut(s) 382, 609, 976, 1077, 1331, 1561
MlyI GAGTC 5 cut(s) 26, 818, 1010, 1017, 1204
Mph1103I ATGCAT 2 cut(s) 419, 457
MseI TTAA 5 cut(s) 144, 345, 495, 1560, 1564
MspCI CTTAAG 1 cut(s) 143
MspR9I CCNGG 1 cut(s) 1381
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 1 cut(s) 1381
MwoI GCNNNNNNNGC 5 cut(s) 111, 322, 331, 473, 1499
NcoI CCATGG 1 cut(s) 188
NdeI CATATG 1 cut(s) 1177
NdeII GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
NlaIV GGNNCC 3 cut(s) 21, 1027, 1124
NsiI ATGCAT 2 cut(s) 419, 457
NspV TTCGAA 1 cut(s) 1299
PacI TTAATTAA 1 cut(s) 1564
PagI TCATGA 2 cut(s) 646, 1188
PciSI GCTCTTC 1 cut(s) 771
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 3 cut(s) 404, 446, 972
PflMI CCANNNNNTGG 1 cut(s) 1045
PkrI GCNGC 4 cut(s) 28, 186, 936, 1495
PleI GAGTC 5 cut(s) 26, 818, 1009, 1016, 1203
PpsI GAGTC 5 cut(s) 26, 818, 1009, 1016, 1203
PpuMI RGGWCCY 1 cut(s) 19
PshBI ATTAAT 2 cut(s) 495, 1560
Psp124BI GAGCTC 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 1379
PspGI CCWGG 1 cut(s) 1379
PspN4I GGNNCC 3 cut(s) 21, 1027, 1124
PspPI GGNCC 4 cut(s) 19, 95, 272, 1036
PspPPI RGGWCCY 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 1045
PsuI RGATCY 1 cut(s) 68
RsaI GTAC 2 cut(s) 1159, 1375
RsaNI GTAC 2 cut(s) 1158, 1374
SacI GAGCTC 1 cut(s) 379
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 5 cut(s) 144, 345, 495, 1560, 1564
SatI GCNGC 4 cut(s) 27, 185, 935, 1494
Sau3AI GATC 7 cut(s) 57, 68, 395, 604, 630, 649, 921
Sau96I GGNCC 4 cut(s) 19, 95, 272, 1036
SchI GAGTC 5 cut(s) 26, 818, 1010, 1017, 1204
ScrFI CCNGG 1 cut(s) 1381
SduI GDGCHC 2 cut(s) 355, 379
SfaNI GCATC 3 cut(s) 232, 442, 694
SfcI CTRYAG 1 cut(s) 205
SfuI TTCGAA 1 cut(s) 1299
SinI GGWCC 2 cut(s) 19, 272
SmlI CTYRAG 3 cut(s) 143, 196, 791
SmoI CTYRAG 3 cut(s) 143, 196, 791
Sse9I AATT 6 cut(s) 382, 609, 976, 1077, 1331, 1561
SsiI CCGC 7 cut(s) 5, 184, 749, 814, 1166, 1420, 1494
SspI AATATT 3 cut(s) 1283, 1296, 1370
SspMI CTAG 4 cut(s) 299, 509, 614, 1310
SstI GAGCTC 1 cut(s) 379
StyD4I CCNGG 1 cut(s) 1379
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 1 cut(s) 646
TaqI TCGA 7 cut(s) 15, 78, 162, 528, 732, 1011, 1299
TasI AATT 6 cut(s) 382, 609, 976, 1077, 1331, 1561
TauI GCSGC 2 cut(s) 187, 1496
TfiI GAWTC 3 cut(s) 404, 446, 972
Tru1I TTAA 5 cut(s) 144, 345, 495, 1560, 1564
Tru9I TTAA 5 cut(s) 144, 345, 495, 1560, 1564
TscAI CASTG 4 cut(s) 241, 789, 1092, 1265
TseI GCWGC 2 cut(s) 26, 934
TspRI CASTG 4 cut(s) 241, 789, 1092, 1265
Van91I CCANNNNNTGG 1 cut(s) 1045
Vha464I CTTAAG 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 19, 272
VspI ATTAAT 2 cut(s) 495, 1560
XapI RAATTY 3 cut(s) 382, 1077, 1331
XbaI TCTAGA 1 cut(s) 613
XmiI GTMKAC 2 cut(s) 204, 1306
XspI CTAG 4 cut(s) 299, 509, 614, 1310
Zsp2I ATGCAT 2 cut(s) 419, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.