Rh5DG131200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000731
Physical Location & Seq
Forward (+)
12821287 .. 12821913
627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG131200.1

Sequence Viewer

Length: 627 bp
ATGGAAAAAGACTTGGAAGAGAAGCTGAAACAGCTCATTGACCAGGGGATTAAAGTTGAGATCGTCAAAGATGGCAACCAGCCAAGAGGCACGTACACTATAGAAGTACATCCCCACACTTTGGAGGTGATGGAAGTTTGGGGCAAGCAGATGAAGATTTTAGAGATGCTGAGTGATCTATACATTCTTGCTGAAGTCAAGTTGACTTTGAATGAGTCCGGTTTGTTGGCATCCTTGTACCAGCACGGCCCAATTGCCACCTGGAGAGCAGTAGCTCAGAGAATGAAAGGGAAAGCTATTGATTCTGCTCATGAGCTAAAGATAACAGAGACATGGCTAGATAGGATTGGACCATTGGCCAAAGAAGTCTCTACTGTTCTCATCGTTCATCAGAAACAAAAGAAGACAGCCTCACAACCTGAAGAAGGTAGCTTAGAGGTCCTCCCCCATGACTTTGGATCCATTTTGATCAAACTTTCCCAACTTCGTTTGAAATGGAAGGTCATTGCTTCTTTGGAGAGTGTAACACTAATGGATGACTCTGAGATGGAAGACATGAATGAGATAGAATCGATAATAAGAGATGTACACAAGGTTGTGGTTGTCCAACAACTGTTGCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.67

Weight (kDa)

5.69

Isoelectric Point (pI)

28.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 121
AclWI GGATC 2 cut(s) 453, 466
AcoI YGGCCR 1 cut(s) 357
AcuI CTGAAG 2 cut(s) 213, 441
AfaI GTAC 4 cut(s) 95, 108, 239, 588
AfiI CCNNNNNNNGG 2 cut(s) 121, 425
AgsI TTSAA 2 cut(s) 211, 493
AjnI CCWGG 2 cut(s) 42, 260
AluBI AGCT 6 cut(s) 25, 34, 275, 296, 316, 432
AluI AGCT 6 cut(s) 25, 34, 275, 296, 316, 432
Alw26I GTCTC 2 cut(s) 323, 373
AlwI GGATC 2 cut(s) 453, 466
AoxI GGCC 2 cut(s) 247, 357
AspS9I GGNCC 3 cut(s) 248, 350, 439
AsuHPI GGTGA 1 cut(s) 139
AvaII GGWCC 2 cut(s) 350, 439
BalI TGGCCA 1 cut(s) 359
BamHI GGATCC 1 cut(s) 458
BbsI GAAGAC 2 cut(s) 410, 558
BccI CCATC 3 cut(s) 65, 124, 541
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 2 cut(s) 44, 262
BclI TGATCA 1 cut(s) 468
BcoDI GTCTC 2 cut(s) 323, 373
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 44, 262
Bme18I GGWCC 2 cut(s) 350, 439
BmgT120I GGNCC 3 cut(s) 248, 350, 439
BmiI GGNNCC 1 cut(s) 460
BmrFI CCNGG 2 cut(s) 44, 262
BmsI GCATC 2 cut(s) 156, 239
BpiI GAAGAC 2 cut(s) 410, 558
BpmI CTGGAG 1 cut(s) 283
Bsa29I ATCGAT 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 218
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 425
Bse3DI GCAATG 1 cut(s) 504
BseBI CCWGG 2 cut(s) 44, 262
BseCI ATCGAT 1 cut(s) 572
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 3 cut(s) 109, 230, 541
BseLI CCNNNNNNNGG 2 cut(s) 121, 425
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 3 cut(s) 161, 290, 534
BshFI GGCC 2 cut(s) 249, 359
BshVI ATCGAT 1 cut(s) 572
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 2 cut(s) 121, 425
BsmAI GTCTC 2 cut(s) 323, 373
BsnI GGCC 2 cut(s) 249, 359
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 4 cut(s) 60, 175, 458, 468
BspANI GGCC 2 cut(s) 249, 359
BspCNI CTCAG 3 cut(s) 162, 289, 535
BspDI ATCGAT 1 cut(s) 572
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 460
BspPI GGATC 2 cut(s) 453, 466
BsrDI GCAATG 1 cut(s) 504
BsrGI TGTACA 1 cut(s) 586
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 4 cut(s) 60, 175, 458, 468
Bst2UI CCWGG 2 cut(s) 44, 262
Bst4CI ACNGT 2 cut(s) 376, 615
Bst6I CTCTTC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 586
BstBAI YACGTR 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 146
BstDEI CTNAG 4 cut(s) 170, 276, 433, 543
BstENI CCTNNNNNAGG 1 cut(s) 423
BstF5I GGATG 3 cut(s) 109, 230, 541
BstKTI GATC 4 cut(s) 63, 178, 461, 471
BstMAI GTCTC 2 cut(s) 323, 373
BstMBI GATC 4 cut(s) 60, 175, 458, 468
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 2 cut(s) 44, 262
BstSCI CCNGG 2 cut(s) 42, 260
BstSFI CTRYAG 1 cut(s) 99
BstV2I GAAGAC 2 cut(s) 410, 558
BstX2I RGATCY 1 cut(s) 458
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 1 cut(s) 458
Bsu15I ATCGAT 1 cut(s) 572
BsuRI GGCC 2 cut(s) 249, 359
BsuTUI ATCGAT 1 cut(s) 572
BtsCI GGATG 3 cut(s) 109, 230, 541
Cac8I GCNNGC 1 cut(s) 146
CciI TCATGA 1 cut(s) 310
Cfr13I GGNCC 3 cut(s) 248, 350, 439
ClaI ATCGAT 1 cut(s) 572
Csp6I GTAC 4 cut(s) 94, 107, 238, 587
CviAII CATG 4 cut(s) 311, 333, 449, 556
CviQI GTAC 4 cut(s) 94, 107, 238, 587
DdeI CTNAG 4 cut(s) 170, 276, 433, 543
DpnI GATC 4 cut(s) 62, 177, 460, 470
DpnII GATC 4 cut(s) 60, 175, 458, 468
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco47I GGWCC 2 cut(s) 350, 439
Eco57I CTGAAG 2 cut(s) 213, 441
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoO109I RGGNCCY 1 cut(s) 439
EcoRII CCWGG 2 cut(s) 42, 260
FaeI CATG 4 cut(s) 314, 336, 452, 559
FaiI YATR 6 cut(s) 101, 181, 312, 334, 450, 557
FatI CATG 4 cut(s) 310, 332, 448, 555
FbaI TGATCA 1 cut(s) 468
FokI GGATG 3 cut(s) 96, 217, 548
FspBI CTAG 1 cut(s) 338
GsuI CTGGAG 1 cut(s) 283
HaeIII GGCC 2 cut(s) 249, 359
HapII CCGG 1 cut(s) 219
Hin1II CATG 4 cut(s) 314, 336, 452, 559
HincII GTYRAC 1 cut(s) 204
HindII GTYRAC 1 cut(s) 204
HinfI GANTC 4 cut(s) 215, 302, 539, 569
HpaII CCGG 1 cut(s) 219
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 3 cut(s) 96, 204, 589
Hpy188I TCNGA 3 cut(s) 279, 393, 544
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 3 cut(s) 96, 204, 589
HpyAV CCTTC 2 cut(s) 419, 493
HpyCH4III ACNGT 2 cut(s) 376, 615
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 1 cut(s) 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 4 cut(s) 170, 276, 433, 543
HpySE526I ACGT 1 cut(s) 92
Hsp92II CATG 4 cut(s) 314, 336, 452, 559
Ksp22I TGATCA 1 cut(s) 468
Kzo9I GATC 4 cut(s) 60, 175, 458, 468
LpnPI CCDG 8 cut(s) 29, 56, 92, 232, 247, 254, 274, 432
LweI GCATC 2 cut(s) 156, 239
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 523
MalI GATC 4 cut(s) 62, 177, 460, 470
MboI GATC 4 cut(s) 60, 175, 458, 468
MboII GAAGA 5 cut(s) 29, 166, 415, 434, 563
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 1 cut(s) 458
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 1 cut(s) 252
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 2 cut(s) 224, 533
MnlI CCTC 5 cut(s) 80, 118, 421, 430, 452
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
MseI TTAA 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 359
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 44, 262
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 2 cut(s) 44, 262
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeII GATC 4 cut(s) 60, 175, 458, 468
NlaIII CATG 4 cut(s) 314, 336, 452, 559
NlaIV GGNNCC 1 cut(s) 460
PagI TCATGA 1 cut(s) 310
PfeI GAWTC 2 cut(s) 302, 569
PflMI CCANNNNNTGG 1 cut(s) 121
PleI GAGTC 2 cut(s) 223, 533
PpsI GAGTC 2 cut(s) 223, 533
Ppu21I YACGTR 1 cut(s) 93
PpuMI RGGWCCY 1 cut(s) 439
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 2 cut(s) 42, 260
PspGI CCWGG 2 cut(s) 42, 260
PspN4I GGNNCC 1 cut(s) 460
PspPI GGNCC 3 cut(s) 248, 350, 439
PspPPI RGGWCCY 1 cut(s) 439
PsuI RGATCY 1 cut(s) 458
RsaI GTAC 4 cut(s) 95, 108, 239, 588
RsaNI GTAC 4 cut(s) 94, 107, 238, 587
SaqAI TTAA 1 cut(s) 51
Sau3AI GATC 4 cut(s) 60, 175, 458, 468
Sau96I GGNCC 3 cut(s) 248, 350, 439
SchI GAGTC 2 cut(s) 224, 533
ScrFI CCNGG 2 cut(s) 44, 262
SfaNI GCATC 2 cut(s) 156, 239
SfcI CTRYAG 1 cut(s) 99
SinI GGWCC 2 cut(s) 350, 439
Sse9I AATT 1 cut(s) 252
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 2 cut(s) 42, 260
TaaI ACNGT 2 cut(s) 376, 615
TaiI ACGT 1 cut(s) 95
TaqI TCGA 1 cut(s) 572
TasI AATT 1 cut(s) 252
TatI WGTACW 2 cut(s) 106, 586
TfiI GAWTC 2 cut(s) 302, 569
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TspDTI ATGAA 4 cut(s) 167, 299, 377, 572
Van91I CCANNNNNTGG 1 cut(s) 121
VpaK11BI GGWCC 2 cut(s) 350, 439
XagI CCTNNNNNAGG 1 cut(s) 423
XcmI CCANNNNNNNNNTGG 1 cut(s) 258
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.