RLG00000023555

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000731
Physical Location & Seq
Forward (+)
25173935 .. 25174534
600 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023555

Sequence Viewer

Length: 600 bp
ATGGGGATTCAATTATTGTGCCTCCGAACTTTTGGAATCTGGAGTCGAGTTTGGCATTGGCTCCAGCCAGGACCAGTTACGTTGCTGGACATAACTTTCCGTGAGGGAGTAATCAGAATTCCACCACTGTTTATTGACGAGACGACGAGTTCATTGTTGAGGAACCTGATTGCTTATGAGCAATGCAGTCTTAGTAGTACGCATGGCGTTACATCATATGCCTGCCTCATGAAGAGTCTCATCGATTCCTCGCGGGATAGCAATCTGCTTCAGGAGAAGGGGATAACAAAATATCACCACTGGATTGGAGGTGAAGATGAATATTTGACTCAAATATTCAGAAGTATACTAGATGAAGTTGTTGTCAAGGAATTCCATTTTAGAACCTTGTGCGATGAAGCGAATGAGTACTGTACATCCTGGTTTCATCTGAGAAAACTCAAAGTATTTCTGAGGGTGCGGATTCAAAGATATAGGAGGCTCCTTTTCAGCACTTATTTTTCTTCTCCGTGGAGTTTCATATCATTCATGGCGGCGCTCACTATTCTCATTCTCACATTTTCAGACATACTATTCCATGCACACAGCTCGTACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

200

Amino Acids

23.43

Weight (kDa)

8.28

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 25 - 185 3.2e-40 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 346
AccII CGCG 1 cut(s) 253
AciI CCGC 3 cut(s) 253, 460, 533
AcsI RAATTY 2 cut(s) 117, 371
AcuI CTGAAG 1 cut(s) 254
AfaI GTAC 4 cut(s) 199, 410, 415, 593
AgsI TTSAA 2 cut(s) 11, 467
AjnI CCWGG 2 cut(s) 67, 419
AluBI AGCT 1 cut(s) 588
AluI AGCT 1 cut(s) 588
Alw26I GTCTC 2 cut(s) 134, 242
ApoI RAATTY 2 cut(s) 117, 371
AspLEI GCGC 1 cut(s) 538
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 287, 323
AvaII GGWCC 1 cut(s) 71
BcgI CGANNNNNNTGC 1 cut(s) 570
BciT130I CCWGG 2 cut(s) 69, 421
BcoDI GTCTC 2 cut(s) 134, 242
BfaI CTAG 1 cut(s) 350
BfoI RGCGCY 1 cut(s) 539
BisI GCNGC 1 cut(s) 534
BlsI GCNGC 1 cut(s) 535
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 2 cut(s) 69, 421
Bme18I GGWCC 1 cut(s) 71
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 3 cut(s) 62, 164, 482
BmrFI CCNGG 2 cut(s) 69, 421
BpmI CTGGAG 2 cut(s) 47, 61
Bsa29I ATCGAT 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 509
Bse1I ACTGG 2 cut(s) 74, 305
Bse3DI GCAATG 1 cut(s) 188
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 2 cut(s) 69, 421
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 509
BseGI GGATG 1 cut(s) 416
BseJI GATNNNNATC 1 cut(s) 261
BseMI GCAATG 1 cut(s) 188
BseMII CTCAG 2 cut(s) 422, 443
BseNI ACTGG 2 cut(s) 74, 305
Bsh1236I CGCG 1 cut(s) 253
BshVI ATCGAT 1 cut(s) 243
BsmAI GTCTC 2 cut(s) 134, 242
BsmBI CGTCTC 1 cut(s) 134
Bsp1407I TGTACA 1 cut(s) 413
BspACI CCGC 3 cut(s) 253, 460, 533
BspCNI CTCAG 2 cut(s) 423, 444
BspDI ATCGAT 1 cut(s) 243
BspFNI CGCG 1 cut(s) 253
BspHI TCATGA 1 cut(s) 228
BspLI GGNNCC 3 cut(s) 62, 164, 482
BsrDI GCAATG 1 cut(s) 188
BsrGI TGTACA 1 cut(s) 413
BsrI ACTGG 2 cut(s) 74, 305
BssECI CCNNGG 1 cut(s) 509
BssNAI GTATAC 1 cut(s) 347
Bst1107I GTATAC 1 cut(s) 347
Bst2UI CCWGG 2 cut(s) 69, 421
Bst4CI ACNGT 2 cut(s) 129, 413
Bst6I CTCTTC 1 cut(s) 227
BstAUI TGTACA 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 3 cut(s) 191, 431, 452
BstDSI CCRYGG 1 cut(s) 509
BstF5I GGATG 1 cut(s) 416
BstFNI CGCG 1 cut(s) 253
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 1 cut(s) 538
BstMAI GTCTC 2 cut(s) 134, 242
BstNI CCWGG 2 cut(s) 69, 421
BstSCI CCNGG 2 cut(s) 67, 419
BstUI CGCG 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 305
BstZ17I GTATAC 1 cut(s) 347
Bsu15I ATCGAT 1 cut(s) 243
BsuTUI ATCGAT 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 509
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 1 cut(s) 416
BtsIMutI CAGTG 2 cut(s) 125, 298
Cac8I GCNNGC 1 cut(s) 223
CciI TCATGA 1 cut(s) 228
CfoI GCGC 1 cut(s) 538
Cfr13I GGNCC 1 cut(s) 71
ClaI ATCGAT 1 cut(s) 243
Csp6I GTAC 4 cut(s) 198, 409, 414, 592
CviAII CATG 4 cut(s) 203, 229, 529, 578
CviJI RGCY 4 cut(s) 61, 67, 481, 588
CviKI_1 RGCY 4 cut(s) 61, 67, 481, 588
CviQI GTAC 4 cut(s) 198, 409, 414, 592
DdeI CTNAG 3 cut(s) 191, 431, 452
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 254
EcoRI GAATTC 2 cut(s) 117, 371
EcoRII CCWGG 2 cut(s) 67, 419
Esp3I CGTCTC 1 cut(s) 134
FaeI CATG 4 cut(s) 206, 232, 532, 581
FatI CATG 4 cut(s) 202, 228, 528, 577
FauI CCCGC 1 cut(s) 246
FauNDI CATATG 1 cut(s) 217
FblI GTMKAC 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 534
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 1 cut(s) 534
FspBI CTAG 1 cut(s) 350
GlaI GCGC 1 cut(s) 537
GluI GCNGC 1 cut(s) 534
GsuI CTGGAG 2 cut(s) 47, 61
HaeII RGCGCY 1 cut(s) 539
HhaI GCGC 1 cut(s) 538
Hin1II CATG 4 cut(s) 206, 232, 532, 581
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HinfI GANTC 7 cut(s) 7, 36, 43, 235, 245, 328, 463
HphI GGTGA 2 cut(s) 287, 323
Hpy166II GTNNAC 1 cut(s) 347
Hpy188I TCNGA 6 cut(s) 26, 116, 341, 432, 453, 565
Hpy188III TCNNGA 3 cut(s) 40, 229, 272
Hpy8I GTNNAC 1 cut(s) 347
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 2 cut(s) 129, 413
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 186, 581
HpyF3I CTNAG 3 cut(s) 191, 431, 452
HpySE526I ACGT 1 cut(s) 80
Hsp92II CATG 4 cut(s) 206, 232, 532, 581
HspAI GCGC 1 cut(s) 536
LmnI GCTCC 2 cut(s) 66, 486
MaeI CTAG 1 cut(s) 350
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 2 cut(s) 76, 208
MboII GAAGA 3 cut(s) 244, 326, 495
MluCI AATT 3 cut(s) 11, 117, 371
MlyI GAGTC 3 cut(s) 52, 244, 322
MnlI CCTC 8 cut(s) 32, 97, 153, 236, 259, 302, 447, 471
MseI TTAA 1 cut(s) 598
MspR9I CCNGG 2 cut(s) 69, 421
MvaI CCWGG 2 cut(s) 69, 421
MvnI CGCG 1 cut(s) 253
NdeI CATATG 1 cut(s) 217
NlaIII CATG 4 cut(s) 206, 232, 532, 581
NlaIV GGNNCC 3 cut(s) 62, 164, 482
PagI TCATGA 1 cut(s) 228
PfeI GAWTC 4 cut(s) 7, 36, 245, 463
PkrI GCNGC 1 cut(s) 535
PleI GAGTC 3 cut(s) 51, 243, 322
PpsI GAGTC 3 cut(s) 51, 243, 322
Psp6I CCWGG 2 cut(s) 67, 419
PspGI CCWGG 2 cut(s) 67, 419
PspN4I GGNNCC 3 cut(s) 62, 164, 482
PspPI GGNCC 1 cut(s) 71
RsaI GTAC 4 cut(s) 199, 410, 415, 593
RsaNI GTAC 4 cut(s) 198, 409, 414, 592
SaqAI TTAA 1 cut(s) 598
SatI GCNGC 1 cut(s) 534
Sau96I GGNCC 1 cut(s) 71
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 3 cut(s) 52, 244, 322
ScrFI CCNGG 2 cut(s) 69, 421
SetI ASST 5 cut(s) 83, 168, 313, 389, 590
SinI GGWCC 1 cut(s) 71
Sse9I AATT 3 cut(s) 11, 117, 371
SsiI CCGC 3 cut(s) 253, 460, 533
SspI AATATT 2 cut(s) 323, 336
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 2 cut(s) 67, 419
TaaI ACNGT 2 cut(s) 129, 413
TaiI ACGT 1 cut(s) 83
TaqI TCGA 2 cut(s) 46, 243
TasI AATT 3 cut(s) 11, 117, 371
TatI WGTACW 2 cut(s) 408, 413
TauI GCSGC 1 cut(s) 536
TfiI GAWTC 4 cut(s) 7, 36, 245, 463
Tru1I TTAA 1 cut(s) 598
Tru9I TTAA 1 cut(s) 598
TscAI CASTG 2 cut(s) 132, 305
TspDTI ATGAA 8 cut(s) 141, 245, 333, 369, 411, 416, 508, 517
TspGWI ACGGA 2 cut(s) 89, 498
TspRI CASTG 2 cut(s) 132, 305
VpaK11BI GGWCC 1 cut(s) 71
XapI RAATTY 2 cut(s) 117, 371
XmiI GTMKAC 1 cut(s) 346
XspI CTAG 1 cut(s) 350
ZrmI AGTACT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.