Rroxscaffold_3G00252610

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000731
Physical Location & Seq
Reverse (-)
46643100 .. 46647206
4107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00252610.1

Sequence Viewer

Length: 1554 bp
ATGGTATCAGAAATTAGTGAGTCCAAGGAGAACACACAGGAAACCCAGGAAGAGCAGGATGCAAAGTGGAAAGCTAAATTAATGAAGGTGATTAGTGCTAAACTCGCAACAGTGAAGGATAAACAGTCCTTGGCCATCACCTTACGCAATAGAAGAATAACAGCGGAGCTGTGCATCTACAGAGTTCCGGAGAAACTGCGAAAAGTGAGGGAGGATGCCTACAAACCCCTTGTTGTCTCAATTGGACCTTTCCATAGAGACACAGTTAGCCTTGCACCAATGTTACAACACAAATTATGTTACATGCTGTCCTTTCTTAATCAGTGTCCTGAAGTTGTAAACGATGCTCAAATATGCCTGGAGAAATGCATAGATAGTATACATGGTTTTGAAAAAATGATTCGTCAATGCTACATCGAAGAGATCAATTGCGAAGATGATGTGCTTGCAGAAATGATGTTAGTTGATGGCTGCTTCATACTGGAACTTTTCCTCAGGTTTCACAAAACGCTAAATTACAAGAAGCTGGAAGAGAAAAAATCTGATCCGGTACTAAAAAGTGCTTGGAATATGACAGCTCTCAAGCATGATCTAGCATTGCTTGAAAACCAGATTCCCTTCTTTATTCTTGAGATTCTGTATGATGCTATCAGGCCTCGTATCATGAAAAAGTGTACAGCACCAGAGTCGGTCAGTAGTCTTGCTCTAAGTTTCTTCAAACCTATGAGCAAAAAGGTGAAGAAGGAAGAGCACACGAGTAAAAAAGAGACTAAGGGCACAGAATGCAAGCATTTACTCGATCTCTTGCACAAATTCTACTTCCTCCCAGCTCCTTCCATGCCGGACTTCTCAAAGGATGTTATTGATTTGGAATCAAATAATGATCCGGAAAATAAACATTGTTCCACAGCGCATCCAGAATCCAGTCTGGAAAAGAAAGATTCACGCCCTGCTGAAGAAAAATGGGGATTCAACTATTGCGCTTCGAAACTTTTGGAGTCTGGAATCGAGTTCCAAGTGTCGTCGAGCACTGAGGAGGAAAGTTTGTTGAACATAACTTTCAATCCGAAGAGTGGGAAAATCCATATACCCAAGCTGGTCATTCATGACGCAACAAGTTTTTCACTCTTCAGGAACTTGATTGCTTTTGAGCAATGCAGTCTTAACAGTACGAACAGCGTCACATCATATGCCTTTCTCATGAGGAGTCTCATCAGTTCCTCACTGGACAGCAAACTGCTTGAGAAAAGAGGGATCATTGAGCACAGTTCTAATGGAGACGAAGAGTATTTGGCTAATAAATTCAAAAGTATACTAGATGGAGTTGATTTGATGGACGACTTTCGTTTTCGGAAGAAGTGCGACGAAGTGAATGAATACCACAAGTCCTGGTACAATCTGAAAAGACTAATAGTATTTTTTCAAAAACAGAGAAGGATCCTGTGGGCCAATTATCTCTCTAGTGCATGGAAGGTTATGTCAATCCTGGCCGCCGTGATCATTCTCATTTTCACTTCCTTACAGACATACTATACAATTCACCCTCGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

517

Amino Acids

59.8

Weight (kDa)

8.06

Isoelectric Point (pI)

51.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 59 - 504 1.4e-101 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 379, 1312
AccIII TCCGGA 2 cut(s) 187, 886
AciI CCGC 2 cut(s) 164, 1491
AclWI GGATC 5 cut(s) 539, 878, 1262, 1432, 1445
AcoI YGGCCR 2 cut(s) 132, 1488
AcsI RAATTY 2 cut(s) 812, 1301
AcuI CTGAAG 3 cut(s) 351, 975, 1114
AfaI GTAC 4 cut(s) 552, 676, 1171, 1394
AfiI CCNNNNNNNGG 1 cut(s) 1073
AgsI TTSAA 8 cut(s) 392, 605, 718, 973, 1051, 1063, 1306, 1424
AjnI CCWGG 4 cut(s) 45, 357, 1388, 1485
AluBI AGCT 6 cut(s) 74, 169, 526, 578, 830, 1096
AluI AGCT 6 cut(s) 74, 169, 526, 578, 830, 1096
Alw21I GWGCWC 3 cut(s) 753, 1031, 1266
Alw26I GTCTC 5 cut(s) 241, 252, 761, 1214, 1272
AlwI GGATC 5 cut(s) 539, 878, 1262, 1432, 1445
Aor13HI TCCGGA 2 cut(s) 187, 886
AoxI GGCC 4 cut(s) 132, 653, 1446, 1488
ApeKI GCWGC 1 cut(s) 471
ApoI RAATTY 2 cut(s) 812, 1301
AseI ATTAAT 1 cut(s) 80
AspLEI GCGC 2 cut(s) 913, 983
AspS9I GGNCC 2 cut(s) 245, 1446
AsuHPI GGTGA 4 cut(s) 100, 130, 748, 1532
AsuII TTCGAA 1 cut(s) 986
AvaII GGWCC 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 779
BalI TGGCCA 1 cut(s) 134
BamHI GGATCC 1 cut(s) 1437
BauI CACGAG 1 cut(s) 754
Bbv12I GWGCWC 3 cut(s) 753, 1031, 1266
BbvI GCAGC 1 cut(s) 458
BccI CCATC 4 cut(s) 143, 461, 1313, 1327
BceAI ACGGC 1 cut(s) 1478
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 4 cut(s) 47, 359, 1390, 1487
BclI TGATCA 1 cut(s) 1497
BcoDI GTCTC 5 cut(s) 241, 252, 761, 1214, 1272
BfaI CTAG 3 cut(s) 593, 1316, 1461
BfmI CTRYAG 1 cut(s) 178
BisI GCNGC 2 cut(s) 472, 1491
BlsI GCNGC 2 cut(s) 473, 1492
Bme1390I CCNGG 4 cut(s) 47, 359, 1390, 1487
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 245, 1446
BmiI GGNNCC 1 cut(s) 1439
BmrFI CCNGG 4 cut(s) 47, 359, 1390, 1487
BmsI GCATC 6 cut(s) 49, 183, 205, 334, 634, 922
BpmI CTGGAG 1 cut(s) 380
Bpu14I TTCGAA 1 cut(s) 986
BpuEI CTTGAG 3 cut(s) 566, 650, 1262
BsaJI CCNNGG 3 cut(s) 24, 45, 129
BsaWI WCCGGW 3 cut(s) 187, 547, 886
Bsc4I CCNNNNNNNGG 1 cut(s) 1073
Bse1I ACTGG 3 cut(s) 486, 924, 1230
Bse21I CCTNAGG 1 cut(s) 494
Bse3DI GCAATG 2 cut(s) 596, 1160
BseAI TCCGGA 2 cut(s) 187, 886
BseBI CCWGG 4 cut(s) 47, 359, 1390, 1487
BseDI CCNNGG 3 cut(s) 24, 45, 129
BseGI GGATG 4 cut(s) 64, 220, 862, 913
BseLI CCNNNNNNNGG 1 cut(s) 1073
BseMI GCAATG 2 cut(s) 596, 1160
BseMII CTCAG 2 cut(s) 508, 1023
BseNI ACTGG 3 cut(s) 486, 924, 1230
BseRI GAGGAG 2 cut(s) 1049, 1219
BseSI GKGCMC 1 cut(s) 779
BseXI GCAGC 1 cut(s) 458
BseYI CCCAGC 1 cut(s) 826
BshFI GGCC 4 cut(s) 134, 655, 1448, 1490
BsiHKAI GWGCWC 3 cut(s) 753, 1031, 1266
BsiSI CCGG 4 cut(s) 188, 548, 842, 887
BslI CCNNNNNNNGG 1 cut(s) 1073
BsmAI GTCTC 5 cut(s) 241, 252, 761, 1214, 1272
BsmBI CGTCTC 1 cut(s) 1272
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 4 cut(s) 134, 655, 1448, 1490
Bsp119I TTCGAA 1 cut(s) 986
Bsp1286I GDGCHC 4 cut(s) 753, 779, 1031, 1266
Bsp13I TCCGGA 2 cut(s) 187, 886
Bsp1407I TGTACA 1 cut(s) 674
Bsp143I GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
BspACI CCGC 2 cut(s) 164, 1491
BspANI GGCC 4 cut(s) 134, 655, 1448, 1490
BspCNI CTCAG 2 cut(s) 507, 1024
BspEI TCCGGA 2 cut(s) 187, 886
BspHI TCATGA 3 cut(s) 663, 1105, 1200
BspLI GGNNCC 1 cut(s) 1439
BspPI GGATC 5 cut(s) 539, 878, 1262, 1432, 1445
BspQI GCTCTTC 2 cut(s) 45, 741
BspT104I TTCGAA 1 cut(s) 986
BsrDI GCAATG 2 cut(s) 596, 1160
BsrGI TGTACA 1 cut(s) 674
BsrI ACTGG 3 cut(s) 486, 924, 1230
BssECI CCNNGG 3 cut(s) 24, 45, 129
BssMI GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
BssNAI GTATAC 2 cut(s) 380, 1313
BssSI CACGAG 1 cut(s) 754
BssT1I CCWWGG 2 cut(s) 24, 129
Bst1107I GTATAC 2 cut(s) 380, 1313
Bst2BI CACGAG 1 cut(s) 754
Bst2UI CCWGG 4 cut(s) 47, 359, 1390, 1487
Bst4CI ACNGT 5 cut(s) 112, 126, 265, 1169, 1268
Bst6I CTCTTC 7 cut(s) 45, 414, 525, 741, 1064, 1133, 1278
BstAPI GCANNNNNTGC 1 cut(s) 783
BstAUI TGTACA 1 cut(s) 674
BstBI TTCGAA 1 cut(s) 986
BstC8I GCNNGC 2 cut(s) 447, 788
BstDEI CTNAG 4 cut(s) 494, 707, 771, 1032
BstF5I GGATG 4 cut(s) 64, 220, 862, 913
BstHHI GCGC 2 cut(s) 913, 983
BstKTI GATC 8 cut(s) 426, 547, 592, 802, 886, 1257, 1440, 1500
BstMAI GTCTC 5 cut(s) 241, 252, 761, 1214, 1272
BstMBI GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
BstMWI GCNNNNNNNGC 2 cut(s) 104, 783
BstNI CCWGG 4 cut(s) 47, 359, 1390, 1487
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 4 cut(s) 45, 357, 1388, 1485
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 779
BstV1I GCAGC 1 cut(s) 458
BstX2I RGATCY 1 cut(s) 1437
BstYI RGATCY 1 cut(s) 1437
BstZ17I GTATAC 2 cut(s) 380, 1313
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 4 cut(s) 134, 655, 1448, 1490
BtsCI GGATG 4 cut(s) 64, 220, 862, 913
BtsIMutI CAGTG 4 cut(s) 117, 329, 1029, 1223
Cac8I GCNNGC 2 cut(s) 447, 788
CciI TCATGA 3 cut(s) 663, 1105, 1200
CfoI GCGC 2 cut(s) 913, 983
Cfr13I GGNCC 2 cut(s) 245, 1446
CseI GACGC 2 cut(s) 1118, 1168
Csp6I GTAC 4 cut(s) 551, 675, 1170, 1393
CviAII CATG 8 cut(s) 304, 383, 587, 664, 838, 1106, 1201, 1467
CviQI GTAC 4 cut(s) 551, 675, 1170, 1393
DdeI CTNAG 4 cut(s) 494, 707, 771, 1032
DpnI GATC 8 cut(s) 425, 546, 591, 801, 885, 1256, 1439, 1499
DpnII GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
EaeI YGGCCR 2 cut(s) 132, 1488
Eam1104I CTCTTC 7 cut(s) 45, 414, 525, 741, 1064, 1133, 1278
EarI CTCTTC 7 cut(s) 45, 414, 525, 741, 1064, 1133, 1278
Eco130I CCWWGG 2 cut(s) 24, 129
Eco147I AGGCCT 1 cut(s) 655
Eco47I GGWCC 1 cut(s) 245
Eco57I CTGAAG 3 cut(s) 351, 975, 1114
Eco81I CCTNAGG 1 cut(s) 494
EcoRII CCWGG 4 cut(s) 45, 357, 1388, 1485
EcoT14I CCWWGG 2 cut(s) 24, 129
EcoT22I ATGCAT 1 cut(s) 371
ErhI CCWWGG 2 cut(s) 24, 129
Esp3I CGTCTC 1 cut(s) 1272
FaeI CATG 8 cut(s) 307, 386, 590, 667, 841, 1109, 1204, 1470
FatI CATG 8 cut(s) 303, 382, 586, 663, 837, 1105, 1200, 1466
FauNDI CATATG 1 cut(s) 1189
FbaI TGATCA 1 cut(s) 1497
FblI GTMKAC 2 cut(s) 379, 1312
Fnu4HI GCNGC 2 cut(s) 472, 1491
FokI GGATG 4 cut(s) 71, 227, 869, 900
Fsp4HI GCNGC 2 cut(s) 472, 1491
FspBI CTAG 3 cut(s) 593, 1316, 1461
GlaI GCGC 2 cut(s) 912, 982
GluI GCNGC 2 cut(s) 472, 1491
GsaI CCCAGC 1 cut(s) 830
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 4 cut(s) 134, 655, 1448, 1490
HapII CCGG 4 cut(s) 188, 548, 842, 887
HgaI GACGC 2 cut(s) 1118, 1168
HhaI GCGC 2 cut(s) 913, 983
Hin1II CATG 8 cut(s) 307, 386, 590, 667, 841, 1109, 1204, 1470
Hin6I GCGC 2 cut(s) 911, 981
HinP1I GCGC 2 cut(s) 911, 981
HpaII CCGG 4 cut(s) 188, 548, 842, 887
HphI GGTGA 4 cut(s) 100, 130, 748, 1532
Hpy166II GTNNAC 4 cut(s) 340, 380, 675, 1313
Hpy188I TCNGA 5 cut(s) 10, 544, 1068, 1353, 1401
Hpy8I GTNNAC 4 cut(s) 340, 380, 675, 1313
Hpy99I CGWCG 2 cut(s) 1027, 1367
HpyAV CCTTC 7 cut(s) 79, 109, 628, 736, 843, 1428, 1465
HpyCH4III ACNGT 5 cut(s) 112, 126, 265, 1169, 1268
HpyCH4V TGCA 9 cut(s) 62, 174, 275, 369, 449, 786, 808, 1158, 1466
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 783
HpyF3I CTNAG 4 cut(s) 494, 707, 771, 1032
Hsp92II CATG 8 cut(s) 307, 386, 590, 667, 841, 1109, 1204, 1470
HspAI GCGC 2 cut(s) 911, 981
Kpn2I TCCGGA 2 cut(s) 187, 886
Ksp22I TGATCA 1 cut(s) 1497
Kzo9I GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
LguI GCTCTTC 2 cut(s) 45, 741
LmnI GCTCC 2 cut(s) 166, 835
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 6 cut(s) 49, 183, 205, 334, 634, 922
MaeI CTAG 3 cut(s) 593, 1316, 1461
MaeIII GTNAC 3 cut(s) 282, 299, 1180
MalI GATC 8 cut(s) 425, 546, 591, 801, 885, 1256, 1439, 1499
MboI GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
MfeI CAATTG 2 cut(s) 240, 427
MflI RGATCY 1 cut(s) 1437
MhlI GDGCHC 4 cut(s) 753, 779, 1031, 1266
MlsI TGGCCA 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 134
MlyI GAGTC 4 cut(s) 29, 695, 1007, 1216
Mox20I TGGCCA 1 cut(s) 134
Mph1103I ATGCAT 1 cut(s) 371
MroI TCCGGA 2 cut(s) 187, 886
MscI TGGCCA 1 cut(s) 134
MseI TTAA 3 cut(s) 80, 318, 1164
Msp20I TGGCCA 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 4 cut(s) 188, 548, 842, 887
MspR9I CCNGG 4 cut(s) 47, 359, 1390, 1487
MunI CAATTG 2 cut(s) 240, 427
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 4 cut(s) 47, 359, 1390, 1487
MwoI GCNNNNNNNGC 2 cut(s) 104, 783
NdeI CATATG 1 cut(s) 1189
NdeII GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
NlaIII CATG 8 cut(s) 307, 386, 590, 667, 841, 1109, 1204, 1470
NlaIV GGNNCC 1 cut(s) 1439
NmuCI GTSAC 1 cut(s) 1180
NsiI ATGCAT 1 cut(s) 371
NspI RCATGY 1 cut(s) 307
NspV TTCGAA 1 cut(s) 986
PagI TCATGA 3 cut(s) 663, 1105, 1200
PceI AGGCCT 1 cut(s) 655
PciSI GCTCTTC 2 cut(s) 45, 741
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 8 cut(s) 400, 613, 634, 872, 920, 941, 969, 1005
PkrI GCNGC 2 cut(s) 473, 1492
PleI GAGTC 4 cut(s) 28, 694, 1006, 1215
PpsI GAGTC 4 cut(s) 28, 694, 1006, 1215
PshBI ATTAAT 1 cut(s) 80
Psp6I CCWGG 4 cut(s) 45, 357, 1388, 1485
PspFI CCCAGC 1 cut(s) 826
PspGI CCWGG 4 cut(s) 45, 357, 1388, 1485
PspN4I GGNNCC 1 cut(s) 1439
PspPI GGNCC 2 cut(s) 245, 1446
PsuI RGATCY 1 cut(s) 1437
RsaI GTAC 4 cut(s) 552, 676, 1171, 1394
RsaNI GTAC 4 cut(s) 551, 675, 1170, 1393
SapI GCTCTTC 2 cut(s) 45, 741
SaqAI TTAA 3 cut(s) 80, 318, 1164
SatI GCNGC 2 cut(s) 472, 1491
Sau3AI GATC 8 cut(s) 423, 544, 589, 799, 883, 1254, 1437, 1497
Sau96I GGNCC 2 cut(s) 245, 1446
SchI GAGTC 4 cut(s) 29, 695, 1007, 1216
ScrFI CCNGG 4 cut(s) 47, 359, 1390, 1487
SduI GDGCHC 4 cut(s) 753, 779, 1031, 1266
SfaNI GCATC 6 cut(s) 49, 183, 205, 334, 634, 922
SfcI CTRYAG 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 986
SinI GGWCC 1 cut(s) 245
SmlI CTYRAG 3 cut(s) 581, 629, 1241
SmoI CTYRAG 3 cut(s) 581, 629, 1241
SseBI AGGCCT 1 cut(s) 655
SsiI CCGC 2 cut(s) 164, 1491
SspMI CTAG 3 cut(s) 593, 1316, 1461
StuI AGGCCT 1 cut(s) 655
StyD4I CCNGG 4 cut(s) 45, 357, 1388, 1485
StyI CCWWGG 2 cut(s) 24, 129
TaaI ACNGT 5 cut(s) 112, 126, 265, 1169, 1268
TaqI TCGA 5 cut(s) 417, 798, 986, 1008, 1025
TaqII GACCGA 1 cut(s) 679
TatI WGTACW 1 cut(s) 674
TauI GCSGC 1 cut(s) 1493
TfiI GAWTC 8 cut(s) 400, 613, 634, 872, 920, 941, 969, 1005
Tru1I TTAA 3 cut(s) 80, 318, 1164
Tru9I TTAA 3 cut(s) 80, 318, 1164
TscAI CASTG 4 cut(s) 117, 329, 1036, 1230
TseFI GTSAC 1 cut(s) 1180
TseI GCWGC 1 cut(s) 471
Tsp45I GTSAC 1 cut(s) 1180
TspDTI ATGAA 5 cut(s) 98, 466, 680, 1094, 1389
TspRI CASTG 4 cut(s) 117, 329, 1036, 1230
VpaK11BI GGWCC 1 cut(s) 245
VspI ATTAAT 1 cut(s) 80
XapI RAATTY 2 cut(s) 812, 1301
XceI RCATGY 1 cut(s) 307
XmiI GTMKAC 2 cut(s) 379, 1312
XspI CTAG 3 cut(s) 593, 1316, 1461
Zsp2I ATGCAT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.