RchiOBHm_Chr5g0017731

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000731
Physical Location & Seq
Forward (+)
12336742 .. 12338001
1260 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29798

Sequence Viewer

Length: 1260 bp
ATGAAACAATATAAATGGTCTTACATGGTATCCCTTTTCCAGCAATCAAAAGATCAAGAAGACACTGAAGCTTCTTGGCGGTGTTTAGAAGCGTGCATCAATGTTATCTATGGATTAGACGAAGTGATTCGTCAATGCTATGCTGAGAAGATCAACTGCACAGAGGATGAGCTTGCAGAAATAATGTTGGTCGATGGTTGCTTCATTCTGGAACTTTTCCTCAGGTTTGACCGAAGCTTAAATTATATGAAGCTTGAAGATTTTGGAATTGATCCAGTACTAAAAAGTCCTTGGATGATCACAGCTCTTCAGCACGATCTTGGATTACTCGAAAACCAAATCCCATTTTTCATTCTTGAACTTCTGTATTATACTATCATGCCTCATGTCATCAATGTCATCAAGTGTGAACCACCGAAATCGGTGACTAGCCTTGCTCTTAAATTCTTTCCACCTATGAGCCAAAAGTCGATAGAAAAAGAGCAGGTGGCGGCAGAGTTTAAGCATTTGCTTGATCTTTTGCATCAATTCTACTACCTCCCAGCTGCAGCTACTGGAGCTCGTGACCTGTCTATCGAGTTCAACGATAGAGCGTCTCTGACTTCTGAGAACGAGCATCGTTCTGCTTCTGCACTATGCAGTCGTGAACTTGATGATCAAGCAACCAATAAAAGTTCGGGATTCAGTTGTTGCGCTTCAGAGCTACTCGAGTCTGGAATTGAGTTCCAAGTCAGGTCAAGTACCGAGGACCAACTGCTGGAAATAACTTTCAGTGAGGGAGTTATCAGAATTCCCCAACTGTACATTCATGAGACCTCCCGTTCACTCTTCAGGAATCTCATTGCTTTCGAGCAATGCAGTATTAGCAGTACCCATGGCGTTACAGCATTCGCCTTCCTTATGGAGAGTCTCATCCGTTCCACGCAAGACATAAATCTGTTCACGAGGAAAAAGATCTTTAGGCACAACTGGATCGGCAGTCAAGAATATTCTACTGCACTGAGAGACACTCTGAAGAAAGTCGTTACCAAGAACGATTCATATTTTGCTACATTATACAAGGAAGTCAATGACTACCGTAAGTCGTACCAGTGGAGCAGACTTCAAGTCTTTTTGAGGGTGCAATATCAAAGACAAAGAGGGATCCTTTGTACTACTTATTTCTCTACTACATGGAAAGTCATATCACTCCTGGTTGCGGTGACCTTTCTCCTTCTCAGTGCCATACAGACGTACTATACAATTAACCCTCACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

419

Amino Acids

48.64

Weight (kDa)

5.76

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 1 - 406 6.5e-81 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 475
Acc36I ACCTGC 1 cut(s) 475
AccB7I CCANNNNNTGG 1 cut(s) 757
AciI CCGC 3 cut(s) 79, 491, 1199
AclWI GGATC 4 cut(s) 266, 980, 1138, 1151
AcsI RAATTY 2 cut(s) 443, 789
AcuI CTGAAG 5 cut(s) 87, 293, 681, 814, 1034
AfaI GTAC 7 cut(s) 279, 742, 803, 871, 1088, 1153, 1235
AfiI CCNNNNNNNGG 2 cut(s) 757, 1198
AgsI TTSAA 4 cut(s) 257, 359, 583, 1106
AhdI GACNNNNNGTC 1 cut(s) 1106
AjnI CCWGG 1 cut(s) 1191
AjuI GAANNNNNNNTTGG 2 cut(s) 789, 821
AluBI AGCT 9 cut(s) 71, 172, 237, 253, 305, 545, 551, 560, 703
AluI AGCT 9 cut(s) 71, 172, 237, 253, 305, 545, 551, 560, 703
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 4 cut(s) 600, 806, 914, 999
AlwI GGATC 4 cut(s) 266, 980, 1138, 1151
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 707
ApeKI GCWGC 2 cut(s) 545, 548
ApoI RAATTY 2 cut(s) 443, 789
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 695
AspS9I GGNCC 1 cut(s) 748
AsuHPI GGTGA 3 cut(s) 436, 1213, 1244
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 1 cut(s) 748
AxyI CCTNAGG 1 cut(s) 221
BamHI GGATCC 1 cut(s) 1143
BanII GRGCYC 1 cut(s) 562
BauI CACGAG 2 cut(s) 561, 943
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 2 cut(s) 532, 560
BccI CCATC 1 cut(s) 188
BciT130I CCWGG 1 cut(s) 1193
BciVI GTATCC 1 cut(s) 40
BclI TGATCA 2 cut(s) 297, 655
BcoDI GTCTC 4 cut(s) 600, 806, 914, 999
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 475
BfuI GTATCC 1 cut(s) 40
BglII AGATCT 1 cut(s) 954
BisI GCNGC 3 cut(s) 492, 546, 549
BlsI GCNGC 3 cut(s) 493, 547, 550
BmcAI AGTACT 1 cut(s) 279
Bme1390I CCNGG 1 cut(s) 1193
Bme18I GGWCC 1 cut(s) 748
BmeRI GACNNNNNGTC 1 cut(s) 1106
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 748
BmiI GGNNCC 1 cut(s) 1145
BmrFI CCNGG 1 cut(s) 1193
BmsI GCATC 3 cut(s) 105, 532, 625
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 2 cut(s) 994, 1026
BpmI CTGGAG 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 806
BsaJI CCNNGG 3 cut(s) 290, 744, 874
Bsc4I CCNNNNNNNGG 2 cut(s) 757, 1198
Bse1I ACTGG 4 cut(s) 275, 559, 974, 1090
Bse21I CCTNAGG 1 cut(s) 221
Bse3DI GCAATG 2 cut(s) 840, 860
BseBI CCWGG 1 cut(s) 1193
BseDI CCNNGG 3 cut(s) 290, 744, 874
BseGI GGATG 3 cut(s) 172, 300, 912
BseLI CCNNNNNNNGG 2 cut(s) 757, 1198
BseMI GCAATG 2 cut(s) 840, 860
BseMII CTCAG 5 cut(s) 135, 235, 597, 992, 1231
BseNI ACTGG 4 cut(s) 275, 559, 974, 1090
BseXI GCAGC 2 cut(s) 532, 560
BseYI CCCAGC 1 cut(s) 541
BsgI GTGCAG 3 cut(s) 142, 615, 981
BsiHKAI GWGCWC 1 cut(s) 562
BsiHKCI CYCGRG 1 cut(s) 707
BslI CCNNNNNNNGG 2 cut(s) 757, 1198
BsmAI GTCTC 4 cut(s) 600, 806, 914, 999
BsmBI CGTCTC 1 cut(s) 600
BsmI GAATGC 1 cut(s) 887
Bso31I GGTCTC 1 cut(s) 806
BsoBI CYCGRG 1 cut(s) 707
Bsp1286I GDGCHC 1 cut(s) 562
Bsp1407I TGTACA 1 cut(s) 801
Bsp19I CCATGG 1 cut(s) 874
BspACI CCGC 3 cut(s) 79, 491, 1199
BspCNI CTCAG 5 cut(s) 136, 234, 598, 993, 1230
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 1 cut(s) 1145
BspMAI CTGCAG 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 4 cut(s) 266, 980, 1138, 1151
BspQI GCTCTTC 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 806
BsrDI GCAATG 2 cut(s) 840, 860
BsrGI TGTACA 1 cut(s) 801
BsrI ACTGG 4 cut(s) 275, 559, 974, 1090
BssECI CCNNGG 3 cut(s) 290, 744, 874
BssSI CACGAG 2 cut(s) 561, 943
BssT1I CCWWGG 2 cut(s) 290, 874
Bst2BI CACGAG 2 cut(s) 561, 943
Bst2UI CCWGG 1 cut(s) 1193
Bst4CI ACNGT 2 cut(s) 801, 1079
Bst6I CTCTTC 2 cut(s) 312, 833
BstAUI TGTACA 1 cut(s) 801
BstC8I GCNNGC 2 cut(s) 94, 174
BstDEI CTNAG 5 cut(s) 144, 221, 606, 1001, 1217
BstDSI CCRYGG 1 cut(s) 874
BstEII GGTNACC 1 cut(s) 1201
BstF5I GGATG 3 cut(s) 172, 300, 912
BstHHI GCGC 1 cut(s) 695
BstMAI GTCTC 4 cut(s) 600, 806, 914, 999
BstMWI GCNNNNNNNGC 2 cut(s) 557, 864
BstNI CCWGG 1 cut(s) 1193
BstPI GGTNACC 1 cut(s) 1201
BstSCI CCNGG 1 cut(s) 1191
BstSFI CTRYAG 1 cut(s) 546
BstV1I GCAGC 2 cut(s) 532, 560
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 2 cut(s) 954, 1143
BstYI RGATCY 2 cut(s) 954, 1143
Bsu36I CCTNAGG 1 cut(s) 221
BsuI GTATCC 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 874
BtsCI GGATG 3 cut(s) 172, 300, 912
BtsIMutI CAGTG 6 cut(s) 63, 778, 998, 1097, 1225, 1255
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 2 cut(s) 94, 174
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 1 cut(s) 808
CfoI GCGC 1 cut(s) 695
Cfr13I GGNCC 1 cut(s) 748
CseI GACGC 1 cut(s) 582
Csp6I GTAC 7 cut(s) 278, 741, 802, 870, 1087, 1152, 1234
CviAII CATG 6 cut(s) 25, 379, 386, 809, 875, 1173
CviQI GTAC 7 cut(s) 278, 741, 802, 870, 1087, 1152, 1234
DdeI CTNAG 5 cut(s) 144, 221, 606, 1001, 1217
DriI GACNNNNNGTC 1 cut(s) 1106
Eam1104I CTCTTC 2 cut(s) 312, 833
Eam1105I GACNNNNNGTC 1 cut(s) 1106
EarI CTCTTC 2 cut(s) 312, 833
Ecl136II GAGCTC 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 290, 874
Eco24I GRGCYC 1 cut(s) 562
Eco31I GGTCTC 1 cut(s) 806
Eco47I GGWCC 1 cut(s) 748
Eco53kI GAGCTC 1 cut(s) 560
Eco57I CTGAAG 5 cut(s) 87, 293, 681, 814, 1034
Eco81I CCTNAGG 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 707
Eco91I GGTNACC 1 cut(s) 1201
EcoICRI GAGCTC 1 cut(s) 560
EcoO65I GGTNACC 1 cut(s) 1201
EcoRI GAATTC 1 cut(s) 789
EcoRII CCWGG 1 cut(s) 1191
EcoT14I CCWWGG 2 cut(s) 290, 874
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 2 cut(s) 290, 874
Esp3I CGTCTC 1 cut(s) 600
FaeI CATG 6 cut(s) 28, 382, 389, 812, 878, 1176
FatI CATG 6 cut(s) 24, 378, 385, 808, 874, 1172
FbaI TGATCA 2 cut(s) 297, 655
Fnu4HI GCNGC 3 cut(s) 492, 546, 549
FokI GGATG 3 cut(s) 179, 307, 899
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 3 cut(s) 492, 546, 549
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 694
GluI GCNGC 3 cut(s) 492, 546, 549
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 1 cut(s) 576
HgaI GACGC 1 cut(s) 582
HhaI GCGC 1 cut(s) 695
Hin1II CATG 6 cut(s) 28, 382, 389, 812, 878, 1176
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HindIII AAGCTT 3 cut(s) 69, 235, 251
HinfI GANTC 6 cut(s) 127, 681, 710, 835, 907, 1037
HphI GGTGA 3 cut(s) 436, 1213, 1244
Hpy166II GTNNAC 4 cut(s) 410, 647, 824, 942
Hpy188I TCNGA 5 cut(s) 600, 607, 700, 788, 1014
Hpy8I GTNNAC 4 cut(s) 410, 647, 824, 942
HpyAV CCTTC 2 cut(s) 904, 1223
HpyCH4III ACNGT 2 cut(s) 801, 1079
HpyCH4IV ACGT 1 cut(s) 1232
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 864
HpyF3I CTNAG 5 cut(s) 144, 221, 606, 1001, 1217
HpySE526I ACGT 1 cut(s) 1232
Hsp92II CATG 6 cut(s) 28, 382, 389, 812, 878, 1176
HspAI GCGC 1 cut(s) 693
Ksp22I TGATCA 2 cut(s) 297, 655
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 557, 1095
Lsp1109I GCAGC 2 cut(s) 532, 560
LweI GCATC 3 cut(s) 105, 532, 625
MaeI CTAG 1 cut(s) 429
MaeII ACGT 1 cut(s) 1232
MaeIII GTNAC 5 cut(s) 424, 563, 880, 1024, 1201
MboII GAAGA 6 cut(s) 71, 160, 269, 299, 820, 1027
MflI RGATCY 2 cut(s) 954, 1143
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 7 cut(s) 241, 267, 443, 527, 717, 789, 1242
MlyI GAGTC 2 cut(s) 719, 916
MroXI GAANNNNTTC 1 cut(s) 126
MseI TTAA 4 cut(s) 239, 441, 501, 1245
MspA1I CMGCKG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 1193
Mva1269I GAATGC 1 cut(s) 887
MvaI CCWGG 1 cut(s) 1193
MwoI GCNNNNNNNGC 2 cut(s) 557, 864
NcoI CCATGG 1 cut(s) 874
NlaIII CATG 6 cut(s) 28, 382, 389, 812, 878, 1176
NlaIV GGNNCC 1 cut(s) 1145
NmuCI GTSAC 3 cut(s) 424, 563, 1201
PaeR7I CTCGAG 1 cut(s) 707
PagI TCATGA 1 cut(s) 808
PaqCI CACCTGC 1 cut(s) 475
PciSI GCTCTTC 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PctI GAATGC 1 cut(s) 887
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 4 cut(s) 127, 681, 835, 1037
PflMI CCANNNNNTGG 1 cut(s) 757
PkrI GCNGC 3 cut(s) 493, 547, 550
PleI GAGTC 2 cut(s) 718, 915
PpsI GAGTC 2 cut(s) 718, 915
Psp124BI GAGCTC 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 1191
PspEI GGTNACC 1 cut(s) 1201
PspFI CCCAGC 1 cut(s) 541
PspGI CCWGG 1 cut(s) 1191
PspN4I GGNNCC 1 cut(s) 1145
PspPI GGNCC 1 cut(s) 748
PspXI VCTCGAGB 1 cut(s) 707
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 2 cut(s) 954, 1143
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 7 cut(s) 279, 742, 803, 871, 1088, 1153, 1235
RsaNI GTAC 7 cut(s) 278, 741, 802, 870, 1087, 1152, 1234
SacI GAGCTC 1 cut(s) 562
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 4 cut(s) 239, 441, 501, 1245
SatI GCNGC 3 cut(s) 492, 546, 549
Sau96I GGNCC 1 cut(s) 748
ScaI AGTACT 1 cut(s) 279
SchI GAGTC 2 cut(s) 719, 916
ScrFI CCNGG 1 cut(s) 1193
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 3 cut(s) 105, 532, 625
SfcI CTRYAG 1 cut(s) 546
Sfr274I CTCGAG 1 cut(s) 707
SinI GGWCC 1 cut(s) 748
SlaI CTCGAG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 707
SmoI CTYRAG 1 cut(s) 707
Sse9I AATT 7 cut(s) 241, 267, 443, 527, 717, 789, 1242
SsiI CCGC 3 cut(s) 79, 491, 1199
SspI AATATT 1 cut(s) 989
SspMI CTAG 1 cut(s) 429
SstI GAGCTC 1 cut(s) 562
StyD4I CCNGG 1 cut(s) 1191
StyI CCWWGG 2 cut(s) 290, 874
TaaI ACNGT 2 cut(s) 801, 1079
TaiI ACGT 1 cut(s) 1235
TaqI TCGA 6 cut(s) 192, 330, 470, 576, 708, 849
TaqII GACCGA 1 cut(s) 246
TasI AATT 7 cut(s) 241, 267, 443, 527, 717, 789, 1242
TatI WGTACW 3 cut(s) 277, 801, 1151
TauI GCSGC 1 cut(s) 494
TfiI GAWTC 4 cut(s) 127, 681, 835, 1037
Tru1I TTAA 4 cut(s) 239, 441, 501, 1245
Tru9I TTAA 4 cut(s) 239, 441, 501, 1245
TscAI CASTG 5 cut(s) 70, 778, 1005, 1097, 1225
TseFI GTSAC 3 cut(s) 424, 563, 1201
TseI GCWGC 2 cut(s) 545, 548
Tsp45I GTSAC 3 cut(s) 424, 563, 1201
TspDTI ATGAA 6 cut(s) 17, 193, 263, 340, 797, 1029
TspGWI ACGGA 1 cut(s) 905
TspRI CASTG 5 cut(s) 70, 778, 1005, 1097, 1225
Van91I CCANNNNNTGG 1 cut(s) 757
VpaK11BI GGWCC 1 cut(s) 748
XapI RAATTY 2 cut(s) 443, 789
XhoI CTCGAG 1 cut(s) 707
XmnI GAANNNNTTC 1 cut(s) 126
XspI CTAG 1 cut(s) 429
ZrmI AGTACT 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.