Rh3BG260900

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000731
Physical Location & Seq
Reverse (-)
25286121 .. 25288122
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG260900.1

Sequence Viewer

Length: 1545 bp
ATGGCGGACGGTATTGATCAAGAGCTTGTGGACTTCGAAAGTCTGCACGAAACCGTCAGCATTGGATCCTTTATACATAAACAGCCAGAGAGCGAGATAAGCTTTACCCAAAAGATGTATCGAAAGAAACTTAAGTGGACCACCGAAATCGAAGAAAAGCTCCACAATGTGCGGCCGTGGAACTTGGGCATCTACAGAGTTCCAAATAGACTTCGTCAAGTCAATGAGGATGCCTACAGCCCTCATGTTGTCTCCATCGGTCCTTTCCATCGACACAAGCCTAACCTAGTAGCCATGAGAGAGCACAAGTTGCGTTATGCGCTATATTTCTTTAAGCAAGCACAAGAAATAGCACAAAAACGAGCTCTAAGTTTCAGCTTTGGATCGCGCGAGTCTTCCGAATGCATGAGAGAGTGGGCGACTGCTATTTATGATTCGGACGCAGAGGTTCGTGGAAGCTACGCTGAAGATATGAGCAACATTGATCAGGAGGAACTAGCAGAAATAATGTTGGTCGATGGTTGCTTCATATTAGAACTTTTCATCAGGTTTTACCTATACGTGCACGGGAAGTTAGAAGGCCCTGAATCTGATCCAATTCTAGAAAGTGCTTGGATGATTGCAGATCTACGGCACGATCTGGCATTGCTTGAAAACCAAATCCCCTTATTTATTATACATGATTTGTATGATGCTATCAGTGGTCATCAGATTCTTGTCGACAATGATTTGCCTCCGCCACATGGTCTTGCTCTTCATTTCTTTCAACCTGTGAGCCTCAAGGCAAGCATTGTAACAGAGTGGAGACATGAGCACAAGCATTTGCTGGATATCTTGCACAAGTTCTATTTCCTTCCGACTAAAACTACGGACTTGTCTATGAGGCTCAATATGGAATTGATCCCTGAGGAACAGCGTCTTAGGGCAGTCCAAAAGAATAAAGTATGGGGATTCAATTATTGTGCCTCCGAACTTTTGGAATCTGGAGTCGAGTTTGGCATTGGCTCCAGCCAGGACCAGTTGCTGGACATAACTTTCCGTGAGGGAGTAATCAGAATTCCACCACTGTTTATCCATGAGACGACGAGTTCATTGTTGAGGAACCTGATTGCTTATGAGCAATGCAGTCTTAGTAGTACGCATGGCGTTACATCATATGCCTGCCTCATGAAGAGTCTCATCGATTCCTCGCGGGATAGCAATCTGCTTCAGGAGAAGGGGATAACAAAATATCACCACTGGATTGGAGGTGAAGATGAATATTTGACTCAAATATTCAGAAGTATACTAGATGAAGTTGTTGTCAAGGAATTCCATTTTAGAACCTTGTGCGATGAAGTGAATGAATACTGTACGTCCTGGTTTCATCTGAGAAAACTCAAAGTATTTCTGAGGGTGCGGATTCAAAGATATAGGAGGCTCCTTTTCAGCACTTATTTTTCTTCTCCATGGAGTTTCATATCATTCATGGCAGCGCTCACCATTCTCATTCTCACATCTTTGCAGACATACTATTCCATGCACACAGCTCGTACTATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

514

Amino Acids

60.06

Weight (kDa)

6.0

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 64 - 498 4e-100 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1024
AccI GTMKAC 2 cut(s) 720, 1285
AccII CGCG 3 cut(s) 388, 390, 1192
AciI CCGC 5 cut(s) 5, 172, 737, 1192, 1399
AclWI GGATC 5 cut(s) 60, 73, 391, 587, 895
AcoI YGGCCR 1 cut(s) 173
AcsI RAATTY 2 cut(s) 1056, 1310
AcuI CTGAAG 2 cut(s) 486, 1193
AdeI CACNNNGTG 1 cut(s) 169
AfaI GTAC 3 cut(s) 1138, 1354, 1534
AfeI AGCGCT 1 cut(s) 1476
AfiI CCNNNNNNNGG 2 cut(s) 743, 1024
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 4 cut(s) 653, 767, 955, 1406
AjnI CCWGG 2 cut(s) 1011, 1358
AluBI AGCT 7 cut(s) 25, 102, 160, 365, 378, 459, 1529
AluI AGCT 7 cut(s) 25, 102, 160, 365, 378, 459, 1529
Alw21I GWGCWC 4 cut(s) 306, 367, 567, 816
Alw26I GTCTC 4 cut(s) 256, 799, 1073, 1181
Alw44I GTGCAC 1 cut(s) 563
AlwI GGATC 5 cut(s) 60, 73, 391, 587, 895
AlwNI CAGNNNCTG 1 cut(s) 1024
Aor51HI AGCGCT 1 cut(s) 1476
AoxI GGCC 2 cut(s) 173, 580
ApaLI GTGCAC 1 cut(s) 563
ApeKI GCWGC 1 cut(s) 1472
ApoI RAATTY 2 cut(s) 1056, 1310
AspLEI GCGC 3 cut(s) 322, 390, 1477
AspS9I GGNCC 4 cut(s) 138, 260, 581, 1015
AsuHPI GGTGA 3 cut(s) 1226, 1262, 1471
AsuII TTCGAA 1 cut(s) 36
AvaII GGWCC 3 cut(s) 138, 260, 1015
AxyI CCTNAGG 1 cut(s) 906
BaeGI GKGCMC 1 cut(s) 567
BamHI GGATCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 4 cut(s) 306, 367, 567, 816
BbvI GCAGC 1 cut(s) 1484
BccI CCATC 3 cut(s) 263, 276, 512
BceAI ACGGC 2 cut(s) 160, 647
BcgI CGANNNNNNTGC 2 cut(s) 1511, 1545
BciT130I CCWGG 2 cut(s) 1013, 1360
BclI TGATCA 2 cut(s) 16, 484
BcoDI GTCTC 4 cut(s) 256, 799, 1073, 1181
BfaI CTAG 4 cut(s) 287, 497, 602, 1289
BfmI CTRYAG 2 cut(s) 193, 235
BfoI RGCGCY 1 cut(s) 1478
BfrI CTTAAG 1 cut(s) 131
BglII AGATCT 1 cut(s) 625
BisI GCNGC 2 cut(s) 173, 1473
BlsI GCNGC 2 cut(s) 174, 1474
Bme1390I CCNGG 2 cut(s) 1013, 1360
Bme18I GGWCC 3 cut(s) 138, 260, 1015
BmgT120I GGNCC 4 cut(s) 138, 260, 581, 1015
BmiI GGNNCC 4 cut(s) 67, 1006, 1103, 1421
BmrFI CCNGG 2 cut(s) 1013, 1360
BmsI GCATC 3 cut(s) 198, 220, 682
BpiI GAAGAC 1 cut(s) 387
BpmI CTGGAG 2 cut(s) 991, 1005
Bpu14I TTCGAA 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 764
Bsa29I ATCGAT 1 cut(s) 1182
BsaAI YACGTR 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 1200
BsaJI CCNNGG 2 cut(s) 176, 1448
Bsc4I CCNNNNNNNGG 2 cut(s) 743, 1024
Bse1I ACTGG 2 cut(s) 1018, 1244
Bse21I CCTNAGG 1 cut(s) 906
Bse3DI GCAATG 2 cut(s) 644, 1127
Bse8I GATNNNNATC 1 cut(s) 1200
BseBI CCWGG 2 cut(s) 1013, 1360
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 2 cut(s) 176, 1448
BseGI GGATG 2 cut(s) 235, 621
BseJI GATNNNNATC 1 cut(s) 1200
BseLI CCNNNNNNNGG 2 cut(s) 743, 1024
BseMI GCAATG 2 cut(s) 644, 1127
BseMII CTCAG 3 cut(s) 897, 1361, 1382
BseNI ACTGG 2 cut(s) 1018, 1244
BseSI GKGCMC 1 cut(s) 567
BseX3I CGGCCG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 1484
BsgI GTGCAG 1 cut(s) 29
Bsh1236I CGCG 3 cut(s) 388, 390, 1192
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 175, 582
BshVI ATCGAT 1 cut(s) 1182
BsiEI CGRYCG 1 cut(s) 176
BsiHKAI GWGCWC 4 cut(s) 306, 367, 567, 816
BslI CCNNNNNNNGG 2 cut(s) 743, 1024
BsmAI GTCTC 4 cut(s) 256, 799, 1073, 1181
BsmBI CGTCTC 1 cut(s) 1073
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 2 cut(s) 175, 582
Bsp119I TTCGAA 1 cut(s) 36
Bsp1286I GDGCHC 4 cut(s) 306, 367, 567, 816
Bsp143I GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
Bsp19I CCATGG 1 cut(s) 1448
BspACI CCGC 5 cut(s) 5, 172, 737, 1192, 1399
BspANI GGCC 2 cut(s) 175, 582
BspCNI CTCAG 3 cut(s) 898, 1362, 1383
BspDI ATCGAT 1 cut(s) 1182
BspFNI CGCG 3 cut(s) 388, 390, 1192
BspHI TCATGA 1 cut(s) 1167
BspLI GGNNCC 4 cut(s) 67, 1006, 1103, 1421
BspPI GGATC 5 cut(s) 60, 73, 391, 587, 895
BspQI GCTCTTC 1 cut(s) 759
BspT104I TTCGAA 1 cut(s) 36
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 644, 1127
BsrI ACTGG 2 cut(s) 1018, 1244
BssECI CCNNGG 2 cut(s) 176, 1448
BssMI GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
BssNAI GTATAC 1 cut(s) 1286
BssT1I CCWWGG 1 cut(s) 1448
Bst1107I GTATAC 1 cut(s) 1286
Bst2UI CCWGG 2 cut(s) 1013, 1360
Bst4CI ACNGT 4 cut(s) 11, 55, 1068, 1352
Bst6I CTCTTC 2 cut(s) 759, 1166
BstAFI CTTAAG 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 310
BstBAI YACGTR 1 cut(s) 562
BstBI TTCGAA 1 cut(s) 36
BstC8I GCNNGC 3 cut(s) 339, 787, 1162
BstDEI CTNAG 6 cut(s) 368, 906, 920, 1130, 1370, 1391
BstDSI CCRYGG 2 cut(s) 176, 1448
BstF5I GGATG 2 cut(s) 235, 621
BstFNI CGCG 3 cut(s) 388, 390, 1192
BstH2I RGCGCY 1 cut(s) 1478
BstHHI GCGC 3 cut(s) 322, 390, 1477
BstKTI GATC 8 cut(s) 19, 68, 386, 487, 595, 628, 640, 903
BstMAI GTCTC 4 cut(s) 256, 799, 1073, 1181
BstMBI GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 3 cut(s) 99, 310, 319
BstNI CCWGG 2 cut(s) 1013, 1360
BstSCI CCNGG 2 cut(s) 1011, 1358
BstSFI CTRYAG 2 cut(s) 193, 235
BstSLI GKGCMC 1 cut(s) 567
BstUI CGCG 3 cut(s) 388, 390, 1192
BstV1I GCAGC 1 cut(s) 1484
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 65, 625
BstXI CCANNNNNNTGG 1 cut(s) 1244
BstYI RGATCY 2 cut(s) 65, 625
BstZ17I GTATAC 1 cut(s) 1286
BstZI CGGCCG 1 cut(s) 173
Bsu15I ATCGAT 1 cut(s) 1182
Bsu36I CCTNAGG 1 cut(s) 906
BsuRI GGCC 2 cut(s) 175, 582
BsuTUI ATCGAT 1 cut(s) 1182
BtgI CCRYGG 2 cut(s) 176, 1448
BtgZI GCGATG 1 cut(s) 1347
BtsCI GGATG 2 cut(s) 235, 621
BtsIMutI CAGTG 3 cut(s) 706, 1064, 1237
Cac8I GCNNGC 3 cut(s) 339, 787, 1162
CaiI CAGNNNCTG 1 cut(s) 1024
CciI TCATGA 1 cut(s) 1167
CfoI GCGC 3 cut(s) 322, 390, 1477
Cfr13I GGNCC 4 cut(s) 138, 260, 581, 1015
ClaI ATCGAT 1 cut(s) 1182
CseI GACGC 2 cut(s) 449, 905
Csp6I GTAC 3 cut(s) 1137, 1353, 1533
CviQI GTAC 3 cut(s) 1137, 1353, 1533
DdeI CTNAG 6 cut(s) 368, 906, 920, 1130, 1370, 1391
DpnI GATC 8 cut(s) 18, 67, 385, 486, 594, 627, 639, 902
DpnII GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
DraIII CACNNNGTG 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 173
EagI CGGCCG 1 cut(s) 173
Eam1104I CTCTTC 2 cut(s) 759, 1166
EarI CTCTTC 2 cut(s) 759, 1166
EciI GGCGGA 2 cut(s) 20, 726
Ecl136II GAGCTC 1 cut(s) 365
EclXI CGGCCG 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 1448
Eco24I GRGCYC 1 cut(s) 367
Eco32I GATATC 1 cut(s) 832
Eco47I GGWCC 3 cut(s) 138, 260, 1015
Eco47III AGCGCT 1 cut(s) 1476
Eco52I CGGCCG 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 365
Eco57I CTGAAG 2 cut(s) 486, 1193
Eco81I CCTNAGG 1 cut(s) 906
EcoICRI GAGCTC 1 cut(s) 365
EcoO109I RGGNCCY 1 cut(s) 581
EcoRI GAATTC 2 cut(s) 1056, 1310
EcoRII CCWGG 2 cut(s) 1011, 1358
EcoRV GATATC 1 cut(s) 832
EcoT14I CCWWGG 1 cut(s) 1448
EcoT22I ATGCAT 1 cut(s) 407
EcoT38I GRGCYC 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 1448
Esp3I CGTCTC 1 cut(s) 1073
FauI CCCGC 1 cut(s) 1185
FauNDI CATATG 1 cut(s) 1156
FbaI TGATCA 2 cut(s) 16, 484
FblI GTMKAC 2 cut(s) 720, 1285
Fnu4HI GCNGC 2 cut(s) 173, 1473
FokI GGATG 2 cut(s) 242, 628
FriOI GRGCYC 1 cut(s) 367
Fsp4HI GCNGC 2 cut(s) 173, 1473
FspBI CTAG 4 cut(s) 287, 497, 602, 1289
GlaI GCGC 3 cut(s) 321, 389, 1476
GluI GCNGC 2 cut(s) 173, 1473
GsuI CTGGAG 2 cut(s) 991, 1005
HaeII RGCGCY 1 cut(s) 1478
HaeIII GGCC 2 cut(s) 175, 582
HgaI GACGC 2 cut(s) 449, 905
HhaI GCGC 3 cut(s) 322, 390, 1477
Hin6I GCGC 3 cut(s) 320, 388, 1475
HinP1I GCGC 3 cut(s) 320, 388, 1475
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HindIII AAGCTT 1 cut(s) 100
HphI GGTGA 3 cut(s) 1226, 1262, 1471
Hpy166II GTNNAC 5 cut(s) 31, 138, 565, 721, 1286
Hpy188III TCNNGA 6 cut(s) 20, 488, 602, 984, 1168, 1211
Hpy8I GTNNAC 5 cut(s) 31, 138, 565, 721, 1286
Hpy99I CGWCG 1 cut(s) 1087
HpyAV CCTTC 3 cut(s) 572, 863, 1210
HpyCH4III ACNGT 4 cut(s) 11, 55, 1068, 1352
HpyCH4IV ACGT 2 cut(s) 561, 1355
HpyCH4V TGCA 8 cut(s) 46, 405, 565, 623, 838, 1125, 1504, 1522
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 310, 319
HpyF3I CTNAG 6 cut(s) 368, 906, 920, 1130, 1370, 1391
HpySE526I ACGT 2 cut(s) 561, 1355
HspAI GCGC 3 cut(s) 320, 388, 1475
Ksp22I TGATCA 2 cut(s) 16, 484
Kzo9I GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 3 cut(s) 165, 1010, 1425
Lsp1109I GCAGC 1 cut(s) 1484
LweI GCATC 3 cut(s) 198, 220, 682
MaeI CTAG 4 cut(s) 287, 497, 602, 1289
MaeII ACGT 2 cut(s) 561, 1355
MaeIII GTNAC 2 cut(s) 793, 1147
MalI GATC 8 cut(s) 18, 67, 385, 486, 594, 627, 639, 902
MboI GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
MboII GAAGA 7 cut(s) 164, 387, 479, 746, 1183, 1265, 1434
MflI RGATCY 2 cut(s) 65, 625
MhlI GDGCHC 4 cut(s) 306, 367, 567, 816
MluCI AATT 6 cut(s) 597, 896, 955, 1056, 1310, 1540
MlyI GAGTC 4 cut(s) 401, 996, 1183, 1261
MmeI TCCRAC 1 cut(s) 881
Mph1103I ATGCAT 1 cut(s) 407
MseI TTAA 3 cut(s) 132, 333, 1543
MspCI CTTAAG 1 cut(s) 131
MspR9I CCNGG 2 cut(s) 1013, 1360
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 2 cut(s) 1013, 1360
MvnI CGCG 3 cut(s) 388, 390, 1192
MwoI GCNNNNNNNGC 3 cut(s) 99, 310, 319
NcoI CCATGG 1 cut(s) 1448
NdeI CATATG 1 cut(s) 1156
NdeII GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
NlaIV GGNNCC 4 cut(s) 67, 1006, 1103, 1421
NsiI ATGCAT 1 cut(s) 407
NspV TTCGAA 1 cut(s) 36
PagI TCATGA 1 cut(s) 1167
PciSI GCTCTTC 1 cut(s) 759
PctI GAATGC 1 cut(s) 407
PfeI GAWTC 7 cut(s) 434, 587, 712, 951, 980, 1184, 1402
PflFI GACNNNGTC 1 cut(s) 213
PflMI CCANNNNNTGG 1 cut(s) 1024
PkrI GCNGC 2 cut(s) 174, 1474
PleI GAGTC 4 cut(s) 400, 995, 1182, 1261
PpsI GAGTC 4 cut(s) 400, 995, 1182, 1261
Ppu21I YACGTR 1 cut(s) 562
Psp124BI GAGCTC 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 1011, 1358
PspGI CCWGG 2 cut(s) 1011, 1358
PspN4I GGNNCC 4 cut(s) 67, 1006, 1103, 1421
PspPI GGNCC 4 cut(s) 138, 260, 581, 1015
PstNI CAGNNNCTG 1 cut(s) 1024
PsuI RGATCY 2 cut(s) 65, 625
PsyI GACNNNGTC 1 cut(s) 213
RsaI GTAC 3 cut(s) 1138, 1354, 1534
RsaNI GTAC 3 cut(s) 1137, 1353, 1533
SacI GAGCTC 1 cut(s) 367
SalI GTCGAC 1 cut(s) 719
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 3 cut(s) 132, 333, 1543
SatI GCNGC 2 cut(s) 173, 1473
Sau3AI GATC 8 cut(s) 16, 65, 383, 484, 592, 625, 637, 900
Sau96I GGNCC 4 cut(s) 138, 260, 581, 1015
SchI GAGTC 4 cut(s) 401, 996, 1183, 1261
ScrFI CCNGG 2 cut(s) 1013, 1360
SduI GDGCHC 4 cut(s) 306, 367, 567, 816
SfaNI GCATC 3 cut(s) 198, 220, 682
SfcI CTRYAG 2 cut(s) 193, 235
SfuI TTCGAA 1 cut(s) 36
SinI GGWCC 3 cut(s) 138, 260, 1015
SmlI CTYRAG 2 cut(s) 131, 779
SmoI CTYRAG 2 cut(s) 131, 779
Sse9I AATT 6 cut(s) 597, 896, 955, 1056, 1310, 1540
SsiI CCGC 5 cut(s) 5, 172, 737, 1192, 1399
SspI AATATT 2 cut(s) 1262, 1275
SspMI CTAG 4 cut(s) 287, 497, 602, 1289
SstI GAGCTC 1 cut(s) 367
StyD4I CCNGG 2 cut(s) 1011, 1358
StyI CCWWGG 1 cut(s) 1448
TaaI ACNGT 4 cut(s) 11, 55, 1068, 1352
TaiI ACGT 2 cut(s) 564, 1358
TaqI TCGA 8 cut(s) 36, 121, 150, 271, 516, 720, 990, 1182
TaqII GACCGA 1 cut(s) 248
TasI AATT 6 cut(s) 597, 896, 955, 1056, 1310, 1540
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 7 cut(s) 434, 587, 712, 951, 980, 1184, 1402
Tru1I TTAA 3 cut(s) 132, 333, 1543
Tru9I TTAA 3 cut(s) 132, 333, 1543
TscAI CASTG 3 cut(s) 706, 1071, 1244
TseI GCWGC 1 cut(s) 1472
TspGWI ACGGA 2 cut(s) 884, 1028
TspRI CASTG 3 cut(s) 706, 1071, 1244
Tth111I GACNNNGTC 1 cut(s) 213
Van91I CCANNNNNTGG 1 cut(s) 1024
Vha464I CTTAAG 1 cut(s) 131
VneI GTGCAC 1 cut(s) 563
VpaK11BI GGWCC 3 cut(s) 138, 260, 1015
XapI RAATTY 2 cut(s) 1056, 1310
XbaI TCTAGA 1 cut(s) 601
XmiI GTMKAC 2 cut(s) 720, 1285
XspI CTAG 4 cut(s) 287, 497, 602, 1289
Zsp2I ATGCAT 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.