Rw0G010540

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00452
OG0000731
Physical Location & Seq
Reverse (-)
48851 .. 54880
6030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010540.1

Sequence Viewer

Length: 1542 bp
ATGAGTGAGGTGAATGAGGAAACTGAGGAGCTAAATGACCCTCCTGAGAGGAGTGAGGTGAAGGAGGAAACTGAGGAGCTAAGTGACCCTGCTGAGAGGAGTGAGGTGAAGGAAGAGGGATGGACAATTAACTTGCATAAGAGAATCAAGGATCAATTGGACAAATTGGATGGTACAGACTGTTTCATCATCCATCGTAAATATAAAGCGGAGTTGTGTATCTATAAAGTTCCGGAGAAACTTCGAAAAGGGAAGGAGGATGCCTACATTCCCCGCGTTGTAGCAATTGGACCTTTCCATCGAGACAAATGTGCACCAATGTCAGAACATAAATTGTATTACATGAAATCCATTCTTAATAATGGTCAAGAAGATACTGACAAATGCTTGACGAAATGCATCCGTGCCATTCAGGAATTGGACGAAGCGATTCGTCATTGCTACAGCGGTAAGGTCGATGATTACGAAAGTGATCAGCTGGCAGAAATGATGTTGGTTGATGGCTGCTTCATGTTGGAACTTTTCCTCAGAACCCACCTTAAATTTGATTATATTTTGGAAAATGTGTACGAATCTGATCCAGTTCTAAAAAGTCATTGGACTATGACGGCTCTCAAGCATGATCTGGCATTGCTTGAAAACCAGATTCCCTTCTTTATTCTCCAAATTCTGTACGATATTCTCCGCCCTTCTGTCAAGGAAAGCTGTGCACCAAAGTCGGCGCCAAAGTCGGTCGCTAGCCTTGCTTTAAATTTCTTCAAGCCTTCTATGAGCCAAAAGAAGATTAAAGAAGAGGCTTCGGTCAGAGAGTCCAAGCATTTGCTTGATCTCTTGCACAAATTCTACTTCCCAGTTCCTGCCGCTCCTGTCTTCTCAACCAATGGCAACGATATAGAATCCAATACCAATCCCAAAAATAAACAAAATGCACGCCCTGCTAAAAATAAATGGGGATTCAATTATTGCGCTTCAAAAATTTTGGAGTCTGGAATCGAGTTCCGAAAGGGGTCGAATACTGAGGAAAGTTTGTTGGACATAACTTTCAATCAGCCAGATGGTATAATCTATATACCAGGACTGCTCATTCACGACTCAACAAGTATCTCACTCTTCAGGAACTTGATTGCTTTTGAGCAATACAGTCTTAGCAGGACGCAAAGCGTCACATCATATGCCTTCCTCATGAGGAGTCTCATCAGTTCCTCACGGGACAGCAGACTGCTTGAGAAAAAAGGGATCATTGAGCACAGTTCTAATGGAAACGAAGAGTATCTGGCTGATAAATTCCGAAGTATACTGGATGGAGTTGACTTGATGGAGTTGGACGACTTCTATTTTCGCGAGCTGTGCGCCACGGTGAACGATGACCACAAGTCCTGGTACAATCTGAAAAGACTTAAAGTATTTTATCAAAAACAGAGACGAATCCTCTGGGCTAATTATCTCTCTAGTGCATGGAAAGTCATGTCAATCCTGGCCGCGGTGACCATTCTCTTTCTCACTTCCTTACAGACATACTATACAATTCACCCTCGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

513

Amino Acids

59.41

Weight (kDa)

6.03

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 74 - 500 3.8e-98 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 721
AccBSI CCGCTC 1 cut(s) 863
AccI GTMKAC 1 cut(s) 1294
AccII CGCG 3 cut(s) 276, 1341, 1481
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 7 cut(s) 209, 274, 447, 685, 861, 1479, 1481
AclWI GGATC 3 cut(s) 159, 572, 1244
AcoI YGGCCR 1 cut(s) 1476
AcsI RAATTY 6 cut(s) 542, 666, 751, 839, 975, 1283
AcuI CTGAAG 1 cut(s) 1096
AcyI GRCGYC 1 cut(s) 722
AfaI GTAC 4 cut(s) 175, 569, 674, 1382
AfiI CCNNNNNNNGG 1 cut(s) 1480
AgsI TTSAA 5 cut(s) 638, 760, 958, 972, 1045
AhdI GACNNNNNGTC 1 cut(s) 1372
AjnI CCWGG 3 cut(s) 1072, 1376, 1473
AluBI AGCT 5 cut(s) 31, 79, 478, 705, 1345
AluI AGCT 5 cut(s) 31, 79, 478, 705, 1345
Alw21I GWGCWC 3 cut(s) 316, 712, 1248
Alw26I GTCTC 3 cut(s) 297, 1196, 1414
Alw44I GTGCAC 2 cut(s) 312, 708
AlwI GGATC 3 cut(s) 159, 572, 1244
AlwNI CAGNNNCTG 1 cut(s) 857
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 1 cut(s) 1476
ApaLI GTGCAC 2 cut(s) 312, 708
ApeKI GCWGC 1 cut(s) 504
ApoI RAATTY 6 cut(s) 542, 666, 751, 839, 975, 1283
Asp700I GAANNNNTTC 1 cut(s) 429
AspLEI GCGC 3 cut(s) 724, 968, 1352
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 6 cut(s) 22, 70, 118, 1369, 1495, 1520
AsuII TTCGAA 1 cut(s) 244
AsuNHI GCTAGC 1 cut(s) 737
AvaII GGWCC 1 cut(s) 290
BaeGI GKGCMC 2 cut(s) 316, 712
BanI GGYRCC 1 cut(s) 721
BbsI GAAGAC 1 cut(s) 862
Bbv12I GWGCWC 3 cut(s) 316, 712, 1248
BbvI GCAGC 1 cut(s) 491
BccI CCATC 8 cut(s) 114, 164, 201, 306, 494, 1049, 1295, 1309
BceAI ACGGC 1 cut(s) 624
BcgI CGANNNNNNTGC 2 cut(s) 699, 733
BciT130I CCWGG 3 cut(s) 1074, 1378, 1475
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 3 cut(s) 297, 1196, 1414
BfaI CTAG 2 cut(s) 738, 1449
BfmI CTRYAG 1 cut(s) 442
BfoI RGCGCY 1 cut(s) 725
BisI GCNGC 3 cut(s) 505, 861, 1479
BlsI GCNGC 3 cut(s) 506, 862, 1480
Bme1390I CCNGG 3 cut(s) 1074, 1378, 1475
Bme18I GGWCC 1 cut(s) 290
BmeRI GACNNNNNGTC 1 cut(s) 1372
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 1 cut(s) 723
BmrFI CCNGG 3 cut(s) 1074, 1378, 1475
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 2 cut(s) 250, 408
BmtI GCTAGC 1 cut(s) 741
BmuI ACTGGG 1 cut(s) 845
BpiI GAAGAC 1 cut(s) 862
Bpu14I TTCGAA 1 cut(s) 244
BpuEI CTTGAG 2 cut(s) 599, 1244
BsaHI GRCGYC 1 cut(s) 722
BsaJI CCNNGG 2 cut(s) 1353, 1479
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 1 cut(s) 1480
Bse1I ACTGG 3 cut(s) 581, 851, 1302
Bse3DI GCAATG 2 cut(s) 436, 629
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 3 cut(s) 1074, 1378, 1475
BseDI CCNNGG 2 cut(s) 1353, 1479
BseGI GGATG 6 cut(s) 125, 175, 189, 265, 399, 1306
BseLI CCNNNNNNNGG 1 cut(s) 1480
BseMI GCAATG 2 cut(s) 436, 629
BseMII CTCAG 6 cut(s) 15, 36, 63, 84, 541, 1008
BseNI ACTGG 3 cut(s) 581, 851, 1302
BseRI GAGGAG 5 cut(s) 41, 64, 89, 112, 1201
BseSI GKGCMC 2 cut(s) 316, 712
BseXI GCAGC 1 cut(s) 491
Bsh1236I CGCG 3 cut(s) 276, 1341, 1481
Bsh1285I CGRYCG 1 cut(s) 735
BshFI GGCC 1 cut(s) 1478
BshNI GGYRCC 1 cut(s) 721
BsiEI CGRYCG 1 cut(s) 735
BsiHKAI GWGCWC 3 cut(s) 316, 712, 1248
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 1 cut(s) 1223
BslI CCNNNNNNNGG 1 cut(s) 1480
BsmAI GTCTC 3 cut(s) 297, 1196, 1414
BsmBI CGTCTC 1 cut(s) 1414
BsmFI GGGAC 1 cut(s) 1223
BsnI GGCC 1 cut(s) 1478
Bsp119I TTCGAA 1 cut(s) 244
Bsp1286I GDGCHC 3 cut(s) 316, 712, 1248
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
Bsp68I TCGCGA 1 cut(s) 1341
BspACI CCGC 7 cut(s) 209, 274, 447, 685, 861, 1479, 1481
BspANI GGCC 1 cut(s) 1478
BspCNI CTCAG 6 cut(s) 16, 37, 64, 85, 540, 1009
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 3 cut(s) 276, 1341, 1481
BspHI TCATGA 1 cut(s) 1182
BspLI GGNNCC 1 cut(s) 723
BspOI GCTAGC 1 cut(s) 741
BspPI GGATC 3 cut(s) 159, 572, 1244
BspT104I TTCGAA 1 cut(s) 244
BspT107I GGYRCC 1 cut(s) 721
BsrBI CCGCTC 1 cut(s) 863
BsrDI GCAATG 2 cut(s) 436, 629
BsrI ACTGG 3 cut(s) 581, 851, 1302
BssECI CCNNGG 2 cut(s) 1353, 1479
BssMI GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
BssNAI GTATAC 1 cut(s) 1295
BssNI GRCGYC 1 cut(s) 722
Bst1107I GTATAC 1 cut(s) 1295
Bst2UI CCWGG 3 cut(s) 1074, 1378, 1475
Bst4CI ACNGT 4 cut(s) 182, 1142, 1250, 1357
Bst6I CTCTTC 4 cut(s) 108, 786, 1115, 1260
BstACI GRCGYC 1 cut(s) 722
BstAPI GCANNNNNTGC 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 480, 739, 931, 1343
BstDEI CTNAG 8 cut(s) 24, 45, 72, 80, 93, 527, 1017, 1145
BstDSI CCRYGG 2 cut(s) 1353, 1479
BstEII GGTNACC 1 cut(s) 1483
BstF5I GGATG 6 cut(s) 125, 175, 189, 265, 399, 1306
BstFNI CGCG 3 cut(s) 276, 1341, 1481
BstH2I RGCGCY 1 cut(s) 725
BstHHI GCGC 3 cut(s) 724, 968, 1352
BstKTI GATC 6 cut(s) 154, 475, 580, 625, 829, 1239
BstMAI GTCTC 3 cut(s) 297, 1196, 1414
BstMBI GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
BstMCI CGRYCG 1 cut(s) 735
BstMWI GCNNNNNNNGC 3 cut(s) 743, 935, 1347
BstNI CCWGG 3 cut(s) 1074, 1378, 1475
BstPI GGTNACC 1 cut(s) 1483
BstSCI CCNGG 3 cut(s) 1072, 1376, 1473
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 2 cut(s) 316, 712
BstUI CGCG 3 cut(s) 276, 1341, 1481
BstV1I GCAGC 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 862
BstZ17I GTATAC 1 cut(s) 1295
BsuRI GGCC 1 cut(s) 1478
BtgI CCRYGG 2 cut(s) 1353, 1479
BtsCI GGATG 6 cut(s) 125, 175, 189, 265, 399, 1306
BtuMI TCGCGA 1 cut(s) 1341
Cac8I GCNNGC 4 cut(s) 480, 739, 931, 1343
CaiI CAGNNNCTG 1 cut(s) 857
CciI TCATGA 1 cut(s) 1182
CfoI GCGC 3 cut(s) 724, 968, 1352
Cfr13I GGNCC 1 cut(s) 290
Cfr42I CCGCGG 1 cut(s) 1482
CseI GACGC 2 cut(s) 1150, 1162
Csp6I GTAC 4 cut(s) 174, 568, 673, 1381
CviAII CATG 6 cut(s) 343, 511, 620, 1183, 1455, 1465
CviQI GTAC 4 cut(s) 174, 568, 673, 1381
DdeI CTNAG 8 cut(s) 24, 45, 72, 80, 93, 527, 1017, 1145
DinI GGCGCC 1 cut(s) 723
DpnI GATC 6 cut(s) 153, 474, 579, 624, 828, 1238
DpnII GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
DraI TTTAAA 1 cut(s) 750
DriI GACNNNNNGTC 1 cut(s) 1372
EaeI YGGCCR 1 cut(s) 1476
Eam1104I CTCTTC 4 cut(s) 108, 786, 1115, 1260
Eam1105I GACNNNNNGTC 1 cut(s) 1372
EarI CTCTTC 4 cut(s) 108, 786, 1115, 1260
EciI GGCGGA 1 cut(s) 674
Eco47I GGWCC 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 1096
Eco91I GGTNACC 1 cut(s) 1483
EcoO65I GGTNACC 1 cut(s) 1483
EcoRII CCWGG 3 cut(s) 1072, 1376, 1473
EcoT22I ATGCAT 1 cut(s) 401
EgeI GGCGCC 1 cut(s) 723
EheI GGCGCC 1 cut(s) 723
Esp3I CGTCTC 1 cut(s) 1414
FaeI CATG 6 cut(s) 346, 514, 623, 1186, 1458, 1468
FaqI GGGAC 1 cut(s) 1223
FatI CATG 6 cut(s) 342, 510, 619, 1182, 1454, 1464
FauI CCCGC 1 cut(s) 281
FauNDI CATATG 1 cut(s) 1171
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 1 cut(s) 1294
Fnu4HI GCNGC 3 cut(s) 505, 861, 1479
FokI GGATG 6 cut(s) 132, 176, 182, 272, 386, 1313
Fsp4HI GCNGC 3 cut(s) 505, 861, 1479
FspBI CTAG 2 cut(s) 738, 1449
GlaI GCGC 3 cut(s) 723, 967, 1351
GluI GCNGC 3 cut(s) 505, 861, 1479
HaeII RGCGCY 1 cut(s) 725
HaeIII GGCC 1 cut(s) 1478
HapII CCGG 1 cut(s) 233
HgaI GACGC 2 cut(s) 1150, 1162
HhaI GCGC 3 cut(s) 724, 968, 1352
Hin1I GRCGYC 1 cut(s) 722
Hin1II CATG 6 cut(s) 346, 514, 623, 1186, 1458, 1468
Hin6I GCGC 3 cut(s) 722, 966, 1350
HinP1I GCGC 3 cut(s) 722, 966, 1350
HincII GTYRAC 1 cut(s) 1309
HindII GTYRAC 1 cut(s) 1309
HpaII CCGG 1 cut(s) 233
HphI GGTGA 6 cut(s) 22, 70, 118, 1369, 1495, 1520
Hpy166II GTNNAC 6 cut(s) 314, 568, 710, 1295, 1309, 1360
Hpy188I TCNGA 7 cut(s) 325, 530, 577, 806, 1001, 1289, 1389
Hpy8I GTNNAC 6 cut(s) 314, 568, 710, 1295, 1309, 1360
HpyAV CCTTC 7 cut(s) 55, 103, 247, 661, 699, 774, 1186
HpyCH4III ACNGT 4 cut(s) 182, 1142, 1250, 1357
HpyCH4V TGCA 7 cut(s) 136, 314, 399, 710, 835, 929, 1454
HpyF10VI GCNNNNNNNGC 3 cut(s) 743, 935, 1347
HpyF3I CTNAG 8 cut(s) 24, 45, 72, 80, 93, 527, 1017, 1145
Hsp92I GRCGYC 1 cut(s) 722
Hsp92II CATG 6 cut(s) 346, 514, 623, 1186, 1458, 1468
HspAI GCGC 3 cut(s) 722, 966, 1350
KasI GGCGCC 1 cut(s) 721
Kpn2I TCCGGA 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 472
KspI CCGCGG 1 cut(s) 1482
Kzo9I GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
LmnI GCTCC 3 cut(s) 28, 76, 868
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 2 cut(s) 250, 408
MaeI CTAG 2 cut(s) 738, 1449
MaeIII GTNAC 3 cut(s) 83, 1162, 1483
MalI GATC 6 cut(s) 153, 474, 579, 624, 828, 1238
MbiI CCGCTC 1 cut(s) 863
MboI GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
MboII GAAGA 8 cut(s) 125, 383, 748, 793, 803, 862, 1102, 1277
MfeI CAATTG 3 cut(s) 155, 285, 1537
MhlI GDGCHC 3 cut(s) 316, 712, 1248
Mly113I GGCGCC 1 cut(s) 722
MlyI GAGTC 4 cut(s) 818, 992, 1085, 1198
MmeI TCCRAC 3 cut(s) 495, 1011, 1302
Mph1103I ATGCAT 1 cut(s) 401
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 429
MseI TTAA 6 cut(s) 129, 357, 540, 749, 786, 1398
MspA1I CMGCKG 3 cut(s) 447, 478, 1481
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 1074, 1378, 1475
MunI CAATTG 3 cut(s) 155, 285, 1537
MvaI CCWGG 3 cut(s) 1074, 1378, 1475
MvnI CGCG 3 cut(s) 276, 1341, 1481
MwoI GCNNNNNNNGC 3 cut(s) 743, 935, 1347
NarI GGCGCC 1 cut(s) 722
NdeI CATATG 1 cut(s) 1171
NdeII GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
NheI GCTAGC 1 cut(s) 737
NlaIII CATG 6 cut(s) 346, 514, 623, 1186, 1458, 1468
NlaIV GGNNCC 1 cut(s) 723
NmuCI GTSAC 3 cut(s) 83, 1162, 1483
NruI TCGCGA 1 cut(s) 1341
NsiI ATGCAT 1 cut(s) 401
NspV TTCGAA 1 cut(s) 244
PagI TCATGA 1 cut(s) 1182
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 8 cut(s) 144, 430, 572, 646, 896, 954, 990, 1425
PkrI GCNGC 3 cut(s) 506, 862, 1480
PleI GAGTC 4 cut(s) 817, 991, 1085, 1197
PluTI GGCGCC 1 cut(s) 725
PpsI GAGTC 4 cut(s) 817, 991, 1085, 1197
Psp6I CCWGG 3 cut(s) 1072, 1376, 1473
PspEI GGTNACC 1 cut(s) 1483
PspGI CCWGG 3 cut(s) 1072, 1376, 1473
PspN4I GGNNCC 1 cut(s) 723
PspPI GGNCC 1 cut(s) 290
PstNI CAGNNNCTG 1 cut(s) 857
PvuII CAGCTG 1 cut(s) 478
RruI TCGCGA 1 cut(s) 1341
RsaI GTAC 4 cut(s) 175, 569, 674, 1382
RsaNI GTAC 4 cut(s) 174, 568, 673, 1381
SacII CCGCGG 1 cut(s) 1482
SaqAI TTAA 6 cut(s) 129, 357, 540, 749, 786, 1398
SatI GCNGC 3 cut(s) 505, 861, 1479
Sau3AI GATC 6 cut(s) 151, 472, 577, 622, 826, 1236
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 4 cut(s) 818, 992, 1085, 1198
ScrFI CCNGG 3 cut(s) 1074, 1378, 1475
SduI GDGCHC 3 cut(s) 316, 712, 1248
SfaNI GCATC 2 cut(s) 250, 408
SfcI CTRYAG 1 cut(s) 442
SfoI GGCGCC 1 cut(s) 723
Sfr303I CCGCGG 1 cut(s) 1482
SfuI TTCGAA 1 cut(s) 244
SgrBI CCGCGG 1 cut(s) 1482
SinI GGWCC 1 cut(s) 290
SmlI CTYRAG 2 cut(s) 614, 1223
SmoI CTYRAG 2 cut(s) 614, 1223
SsiI CCGC 7 cut(s) 209, 274, 447, 685, 861, 1479, 1481
SspDI GGCGCC 1 cut(s) 721
SspMI CTAG 2 cut(s) 738, 1449
StyD4I CCNGG 3 cut(s) 1072, 1376, 1473
TaaI ACNGT 4 cut(s) 182, 1142, 1250, 1357
TaqI TCGA 5 cut(s) 244, 301, 456, 993, 1010
TaqII GACCGA 2 cut(s) 721, 790
TauI GCSGC 2 cut(s) 863, 1481
TfiI GAWTC 8 cut(s) 144, 430, 572, 646, 896, 954, 990, 1425
Tru1I TTAA 6 cut(s) 129, 357, 540, 749, 786, 1398
Tru9I TTAA 6 cut(s) 129, 357, 540, 749, 786, 1398
TseFI GTSAC 3 cut(s) 83, 1162, 1483
TseI GCWGC 1 cut(s) 504
Tsp45I GTSAC 3 cut(s) 83, 1162, 1483
TspDTI ATGAA 3 cut(s) 175, 359, 499
TspGWI ACGGA 1 cut(s) 392
VneI GTGCAC 2 cut(s) 312, 708
VpaK11BI GGWCC 1 cut(s) 290
XapI RAATTY 6 cut(s) 542, 666, 751, 839, 975, 1283
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XmiI GTMKAC 1 cut(s) 1294
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 2 cut(s) 738, 1449
Zsp2I ATGCAT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.