RLG00000023557

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000731
Physical Location & Seq
Forward (+)
25184803 .. 25187025
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023557

Sequence Viewer

Length: 1308 bp
ATGTATCGAAAGAAACTTAAGTGGACCACGGACATCGAAGAAAAGCTCCACAATGTGCGGCCGTGGAACTTGAGCATCTACAGAGTTCCAATAGACTTCGTCAAGTCAATGAGGACGCCTACAGCCCTCATGTTGTCTCCATCGCGAGCACAAGAAATAGCACAAAAACGAGCTCTACGAGGTTTCAGCTTTGAATCGCGCGAGTCTTCCGAATGCATGAGAGAGTGGGCGACTGCTATTTATGATTCGGACGCAGAGGTTCGTGGAAGCTACGCTGAAGATATGAGCAACATTGATCAGGAGGAACTAGCAGAAATAATGTTGGTCGATGGTTGCTTCTTATTGGAACTTTTCATCAAGTTTTACCTGTACGTGCGCGGGAAGTTAGAAGGCCCTGAATCTGATCCAATTCTAGAAAGTGCTTGGATGATTGCAGATCTACGGCACGATCTGGCATTGCTTGAGAACCAAATCCCCTTTTGTATTATACTTGATTTGTATGATGCTATTAGTGGTCATCAGATACTTGTCGACAATGAGTTGCCTCCACCACATGGTCTTGCTCTTCACTTCTTTCAACCTGTGAGCCTCAAGGAAAGCATAGTAACAGAGTGGAGACCCGAGTACAAGCATTTGCTGGATATCTTGCACAAGTTCTATTTCCTCCCAACTAAAACTACCGACTTGTCTATGAGGCTCAATATGGAATTGATCCCTGAGGAGCAGCGTCTTAGGGCAGTCCAAAAGAATAAAGTATGGGGATTCAATTATTGTGCCTCCGAACTTTTGGAGTCCGGAGTCGAGTTTGGAATTGGCTCCAGCCACAACCAGTTGCTGGAAATAACTTTCAGTGAAGGAGTAATCAGAATTCCACCACTGTTTATTCACGGGACGACGAGTTCATTGTTGAGGAACCTTATTGCTTATGAGCAATGCAGTCTTAGTAGTACGCATGGCGTTACATCATATGCCTGCCTCATGAAGAGTCTCATCGATTCCTCGCGGGATAGCAATCTGCTTCAGGAGAGGGGGATAACAAAAAATCACCACTGGATTGGAGGTGAAGATGAATATTTGACTCAAATATTCAAAGGTATACTAGATGAAGTGGTTGTCAAGGAATTCCATTTTAGAACCTTGTGCGATGAAGTGAATGAATACTGTACATCCTGGTTTCATCTGAGAAAACTCAAGGAAATATCGCCGATAGATTGGACACACACGGCGATAAGCTTCATCTCTGCAGCGGTCCGCCGTTTGGTTAAACCCTTAAACCTCAAAATGCCGCCATCTGGTATCGCCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

436

Amino Acids

50.21

Weight (kDa)

5.65

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 52 - 396 1.7e-70 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 554, 835
AccI GTMKAC 2 cut(s) 531, 1096
AccII CGCG 5 cut(s) 145, 199, 201, 378, 1003
AccIII TCCGGA 1 cut(s) 794
AciI CCGC 6 cut(s) 58, 378, 1003, 1247, 1252, 1286
AclWI GGATC 2 cut(s) 398, 706
AcoI YGGCCR 1 cut(s) 59
AcsI RAATTY 2 cut(s) 867, 1121
AcuI CTGAAG 2 cut(s) 297, 1004
AcyI GRCGYC 1 cut(s) 116
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 4 cut(s) 371, 626, 949, 1165
AfiI CCNNNNNNNGG 4 cut(s) 554, 835, 1258, 1292
AflII CTTAAG 1 cut(s) 17
AgsI TTSAA 4 cut(s) 194, 578, 766, 1090
AjnI CCWGG 1 cut(s) 1169
AloI GAACNNNNNNTCC 2 cut(s) 883, 915
AluBI AGCT 5 cut(s) 46, 173, 189, 270, 1233
AluI AGCT 5 cut(s) 46, 173, 189, 270, 1233
Alw21I GWGCWC 2 cut(s) 151, 175
Alw26I GTCTC 3 cut(s) 141, 610, 992
AlwI GGATC 2 cut(s) 398, 706
AlwNI CAGNNNCTG 1 cut(s) 835
Ama87I CYCGRG 1 cut(s) 620
Aor13HI TCCGGA 1 cut(s) 794
AoxI GGCC 2 cut(s) 59, 391
ApeKI GCWGC 2 cut(s) 724, 1244
ApoI RAATTY 2 cut(s) 867, 1121
AspLEI GCGC 2 cut(s) 201, 378
AspS9I GGNCC 3 cut(s) 24, 392, 1249
AsuHPI GGTGA 2 cut(s) 1037, 1073
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 2 cut(s) 24, 1249
AxyI CCTNAGG 1 cut(s) 717
BanII GRGCYC 1 cut(s) 175
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 151, 175
BbvI GCAGC 2 cut(s) 736, 1256
BccI CCATC 3 cut(s) 148, 323, 1297
BceAI ACGGC 4 cut(s) 46, 458, 1239, 1239
BciT130I CCWGG 1 cut(s) 1171
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 3 cut(s) 141, 610, 992
BfaI CTAG 3 cut(s) 308, 413, 1100
BfmI CTRYAG 3 cut(s) 79, 120, 1242
BfrI CTTAAG 1 cut(s) 17
BglII AGATCT 1 cut(s) 436
BisI GCNGC 4 cut(s) 59, 725, 1245, 1286
BlsI GCNGC 4 cut(s) 60, 726, 1246, 1287
Bme1390I CCNGG 1 cut(s) 1171
Bme18I GGWCC 2 cut(s) 24, 1249
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 3 cut(s) 24, 392, 1249
BmiI GGNNCC 2 cut(s) 817, 914
BmrFI CCNGG 1 cut(s) 1171
BmsI GCATC 2 cut(s) 84, 493
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 802
BpuEI CTTGAG 4 cut(s) 91, 482, 575, 1175
Bsa29I ATCGAT 1 cut(s) 993
BsaAI YACGTR 1 cut(s) 373
BsaBI GATNNNNATC 1 cut(s) 1011
BsaHI GRCGYC 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 2 cut(s) 27, 62
BsaWI WCCGGW 1 cut(s) 794
Bsc4I CCNNNNNNNGG 4 cut(s) 554, 835, 1258, 1292
Bse1I ACTGG 2 cut(s) 829, 1055
Bse21I CCTNAGG 1 cut(s) 717
Bse3DI GCAATG 2 cut(s) 455, 938
Bse8I GATNNNNATC 1 cut(s) 1011
BseAI TCCGGA 1 cut(s) 794
BseBI CCWGG 1 cut(s) 1171
BseCI ATCGAT 1 cut(s) 993
BseDI CCNNGG 2 cut(s) 27, 62
BseGI GGATG 2 cut(s) 432, 1166
BseJI GATNNNNATC 1 cut(s) 1011
BseLI CCNNNNNNNGG 4 cut(s) 554, 835, 1258, 1292
BseMI GCAATG 2 cut(s) 455, 938
BseMII CTCAG 2 cut(s) 708, 1172
BseNI ACTGG 2 cut(s) 829, 1055
BseRI GAGGAG 1 cut(s) 734
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 736, 1256
Bsh1236I CGCG 5 cut(s) 145, 199, 201, 378, 1003
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 61, 393
BshVI ATCGAT 1 cut(s) 993
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 151, 175
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 1 cut(s) 795
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 4 cut(s) 554, 835, 1258, 1292
BsmAI GTCTC 3 cut(s) 141, 610, 992
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 2 cut(s) 61, 393
Bso31I GGTCTC 1 cut(s) 610
BsoBI CYCGRG 1 cut(s) 620
Bsp1286I GDGCHC 2 cut(s) 151, 175
Bsp13I TCCGGA 1 cut(s) 794
Bsp1407I TGTACA 1 cut(s) 1163
Bsp143I GATC 5 cut(s) 295, 403, 436, 448, 711
Bsp68I TCGCGA 1 cut(s) 145
BspACI CCGC 6 cut(s) 58, 378, 1003, 1247, 1252, 1286
BspANI GGCC 2 cut(s) 61, 393
BspCNI CTCAG 2 cut(s) 709, 1173
BspDI ATCGAT 1 cut(s) 993
BspEI TCCGGA 1 cut(s) 794
BspFNI CGCG 5 cut(s) 145, 199, 201, 378, 1003
BspHI TCATGA 1 cut(s) 978
BspLI GGNNCC 2 cut(s) 817, 914
BspMAI CTGCAG 1 cut(s) 1246
BspPI GGATC 2 cut(s) 398, 706
BspQI GCTCTTC 1 cut(s) 570
BspTI CTTAAG 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 610
BsrDI GCAATG 2 cut(s) 455, 938
BsrGI TGTACA 1 cut(s) 1163
BsrI ACTGG 2 cut(s) 829, 1055
BssECI CCNNGG 2 cut(s) 27, 62
BssMI GATC 5 cut(s) 295, 403, 436, 448, 711
BssNAI GTATAC 1 cut(s) 1097
BssNI GRCGYC 1 cut(s) 116
Bst1107I GTATAC 1 cut(s) 1097
Bst2UI CCWGG 1 cut(s) 1171
Bst4CI ACNGT 2 cut(s) 879, 1163
Bst6I CTCTTC 2 cut(s) 570, 977
BstACI GRCGYC 1 cut(s) 116
BstAFI CTTAAG 1 cut(s) 17
BstAUI TGTACA 1 cut(s) 1163
BstBAI YACGTR 1 cut(s) 373
BstC8I GCNNGC 2 cut(s) 147, 973
BstDEI CTNAG 4 cut(s) 717, 731, 941, 1181
BstDSI CCRYGG 2 cut(s) 27, 62
BstF5I GGATG 2 cut(s) 432, 1166
BstFNI CGCG 5 cut(s) 145, 199, 201, 378, 1003
BstHHI GCGC 2 cut(s) 201, 378
BstKTI GATC 5 cut(s) 298, 406, 439, 451, 714
BstMAI GTCTC 3 cut(s) 141, 610, 992
BstMBI GATC 5 cut(s) 295, 403, 436, 448, 711
BstMCI CGRYCG 1 cut(s) 62
BstNI CCWGG 1 cut(s) 1171
BstSCI CCNGG 1 cut(s) 1169
BstSFI CTRYAG 3 cut(s) 79, 120, 1242
BstUI CGCG 5 cut(s) 145, 199, 201, 378, 1003
BstV1I GCAGC 2 cut(s) 736, 1256
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 436
BstXI CCANNNNNNTGG 1 cut(s) 1055
BstYI RGATCY 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 1097
BstZI CGGCCG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 993
Bsu36I CCTNAGG 1 cut(s) 717
BsuRI GGCC 2 cut(s) 61, 393
BsuTUI ATCGAT 1 cut(s) 993
BtgI CCRYGG 2 cut(s) 27, 62
BtgZI GCGATG 2 cut(s) 126, 1158
BtsCI GGATG 2 cut(s) 432, 1166
BtsIMutI CAGTG 3 cut(s) 856, 875, 1048
BtuMI TCGCGA 1 cut(s) 145
Cac8I GCNNGC 2 cut(s) 147, 973
CaiI CAGNNNCTG 1 cut(s) 835
CciI TCATGA 1 cut(s) 978
CfoI GCGC 2 cut(s) 201, 378
Cfr13I GGNCC 3 cut(s) 24, 392, 1249
ClaI ATCGAT 1 cut(s) 993
CpoI CGGWCCG 1 cut(s) 1249
CseI GACGC 3 cut(s) 124, 260, 716
Csp6I GTAC 4 cut(s) 370, 625, 948, 1164
CspI CGGWCCG 1 cut(s) 1249
CviAII CATG 5 cut(s) 130, 217, 554, 953, 979
CviQI GTAC 4 cut(s) 370, 625, 948, 1164
DdeI CTNAG 4 cut(s) 717, 731, 941, 1181
DpnI GATC 5 cut(s) 297, 405, 438, 450, 713
DpnII GATC 5 cut(s) 295, 403, 436, 448, 711
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 59
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 2 cut(s) 570, 977
EarI CTCTTC 2 cut(s) 570, 977
EciI GGCGGA 1 cut(s) 1241
Ecl136II GAGCTC 1 cut(s) 173
EclXI CGGCCG 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 610
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 2 cut(s) 24, 1249
Eco52I CGGCCG 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 173
Eco57I CTGAAG 2 cut(s) 297, 1004
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 620
EcoICRI GAGCTC 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 867, 1121
EcoRII CCWGG 1 cut(s) 1169
EcoRV GATATC 1 cut(s) 643
EcoT22I ATGCAT 1 cut(s) 218
EcoT38I GRGCYC 1 cut(s) 175
FaeI CATG 5 cut(s) 133, 220, 557, 956, 982
FaqI GGGAC 1 cut(s) 904
FatI CATG 5 cut(s) 129, 216, 553, 952, 978
FauI CCCGC 2 cut(s) 371, 996
FauNDI CATATG 1 cut(s) 967
FbaI TGATCA 1 cut(s) 295
FblI GTMKAC 2 cut(s) 531, 1096
Fnu4HI GCNGC 4 cut(s) 59, 725, 1245, 1286
FokI GGATG 2 cut(s) 439, 1153
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 4 cut(s) 59, 725, 1245, 1286
FspBI CTAG 3 cut(s) 308, 413, 1100
GlaI GCGC 2 cut(s) 200, 377
GluI GCNGC 4 cut(s) 59, 725, 1245, 1286
GsuI CTGGAG 1 cut(s) 802
HaeIII GGCC 2 cut(s) 61, 393
HapII CCGG 1 cut(s) 795
HgaI GACGC 3 cut(s) 124, 260, 716
HhaI GCGC 2 cut(s) 201, 378
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 5 cut(s) 133, 220, 557, 956, 982
Hin6I GCGC 2 cut(s) 199, 376
HinP1I GCGC 2 cut(s) 199, 376
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 1 cut(s) 1231
HpaII CCGG 1 cut(s) 795
HphI GGTGA 2 cut(s) 1037, 1073
Hpy166II GTNNAC 3 cut(s) 24, 532, 1097
Hpy188I TCNGA 7 cut(s) 211, 250, 403, 522, 781, 866, 1182
Hpy188III TCNNGA 6 cut(s) 144, 299, 413, 795, 979, 1022
Hpy8I GTNNAC 3 cut(s) 24, 532, 1097
Hpy99I CGWCG 1 cut(s) 898
HpyAV CCTTC 2 cut(s) 383, 848
HpyCH4III ACNGT 2 cut(s) 879, 1163
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 5 cut(s) 216, 434, 649, 936, 1244
HpyF3I CTNAG 4 cut(s) 717, 731, 941, 1181
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 5 cut(s) 133, 220, 557, 956, 982
HspAI GCGC 2 cut(s) 199, 376
Kpn2I TCCGGA 1 cut(s) 794
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 5 cut(s) 295, 403, 436, 448, 711
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 3 cut(s) 51, 721, 821
Lsp1109I GCAGC 2 cut(s) 736, 1256
LweI GCATC 2 cut(s) 84, 493
MaeI CTAG 3 cut(s) 308, 413, 1100
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 604, 958
MalI GATC 5 cut(s) 297, 405, 438, 450, 713
MboI GATC 5 cut(s) 295, 403, 436, 448, 711
MboII GAAGA 6 cut(s) 50, 198, 290, 557, 994, 1076
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 151, 175
MluCI AATT 6 cut(s) 408, 707, 766, 810, 867, 1121
MlyI GAGTC 5 cut(s) 212, 800, 807, 994, 1072
Mph1103I ATGCAT 1 cut(s) 218
MroI TCCGGA 1 cut(s) 794
MseI TTAA 4 cut(s) 18, 1263, 1271, 1306
MspA1I CMGCKG 1 cut(s) 1247
MspCI CTTAAG 1 cut(s) 17
MspI CCGG 1 cut(s) 795
MspR9I CCNGG 1 cut(s) 1171
Mva1269I GAATGC 1 cut(s) 218
MvaI CCWGG 1 cut(s) 1171
MvnI CGCG 5 cut(s) 145, 199, 201, 378, 1003
NdeI CATATG 1 cut(s) 967
NdeII GATC 5 cut(s) 295, 403, 436, 448, 711
NlaIII CATG 5 cut(s) 133, 220, 557, 956, 982
NlaIV GGNNCC 2 cut(s) 817, 914
NruI TCGCGA 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 218
PagI TCATGA 1 cut(s) 978
PciSI GCTCTTC 1 cut(s) 570
PcsI WCGNNNNNNNCGW 1 cut(s) 175
PctI GAATGC 1 cut(s) 218
PfeI GAWTC 5 cut(s) 194, 245, 398, 762, 995
PflFI GACNNNGTC 1 cut(s) 98
PflMI CCANNNNNTGG 2 cut(s) 554, 835
PkrI GCNGC 4 cut(s) 60, 726, 1246, 1287
PleI GAGTC 5 cut(s) 211, 799, 806, 993, 1072
PpsI GAGTC 5 cut(s) 211, 799, 806, 993, 1072
Ppu21I YACGTR 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 1169
PspGI CCWGG 1 cut(s) 1169
PspN4I GGNNCC 2 cut(s) 817, 914
PspPI GGNCC 3 cut(s) 24, 392, 1249
PstI CTGCAG 1 cut(s) 1246
PstNI CAGNNNCTG 1 cut(s) 835
PsuI RGATCY 1 cut(s) 436
PsyI GACNNNGTC 1 cut(s) 98
RruI TCGCGA 1 cut(s) 145
RsaI GTAC 4 cut(s) 371, 626, 949, 1165
RsaNI GTAC 4 cut(s) 370, 625, 948, 1164
Rsr2I CGGWCCG 1 cut(s) 1249
RsrII CGGWCCG 1 cut(s) 1249
SacI GAGCTC 1 cut(s) 175
SalI GTCGAC 1 cut(s) 530
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 4 cut(s) 18, 1263, 1271, 1306
SatI GCNGC 4 cut(s) 59, 725, 1245, 1286
Sau3AI GATC 5 cut(s) 295, 403, 436, 448, 711
Sau96I GGNCC 3 cut(s) 24, 392, 1249
SchI GAGTC 5 cut(s) 212, 800, 807, 994, 1072
ScrFI CCNGG 1 cut(s) 1171
SduI GDGCHC 2 cut(s) 151, 175
SfaNI GCATC 2 cut(s) 84, 493
SfcI CTRYAG 3 cut(s) 79, 120, 1242
SinI GGWCC 2 cut(s) 24, 1249
SmlI CTYRAG 5 cut(s) 17, 70, 461, 590, 1190
SmoI CTYRAG 5 cut(s) 17, 70, 461, 590, 1190
Sse9I AATT 6 cut(s) 408, 707, 766, 810, 867, 1121
SsiI CCGC 6 cut(s) 58, 378, 1003, 1247, 1252, 1286
SspI AATATT 2 cut(s) 1073, 1086
SspMI CTAG 3 cut(s) 308, 413, 1100
SstI GAGCTC 1 cut(s) 175
StyD4I CCNGG 1 cut(s) 1169
TaaI ACNGT 2 cut(s) 879, 1163
TaiI ACGT 1 cut(s) 375
TaqI TCGA 6 cut(s) 7, 36, 327, 531, 801, 993
TasI AATT 6 cut(s) 408, 707, 766, 810, 867, 1121
TatI WGTACW 2 cut(s) 624, 1163
TauI GCSGC 2 cut(s) 61, 1288
TfiI GAWTC 5 cut(s) 194, 245, 398, 762, 995
Tru1I TTAA 4 cut(s) 18, 1263, 1271, 1306
Tru9I TTAA 4 cut(s) 18, 1263, 1271, 1306
TscAI CASTG 3 cut(s) 856, 882, 1055
TseI GCWGC 2 cut(s) 724, 1244
TspDTI ATGAA 9 cut(s) 343, 891, 995, 1083, 1119, 1161, 1166, 1170, 1225
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 3 cut(s) 856, 882, 1055
Tth111I GACNNNGTC 1 cut(s) 98
Van91I CCANNNNNTGG 2 cut(s) 554, 835
Vha464I CTTAAG 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 24, 1249
XapI RAATTY 2 cut(s) 867, 1121
XbaI TCTAGA 1 cut(s) 412
XmiI GTMKAC 2 cut(s) 531, 1096
XspI CTAG 3 cut(s) 308, 413, 1100
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.