RLG00000023554

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000731
Physical Location & Seq
Forward (+)
25173157 .. 25173840
684 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023554

Sequence Viewer

Length: 684 bp
ATGTCCGGCCGTGGGAACTTAAGCATCTACAGAGTTCCAAATAGACTTCGTCAAGTCGATGAGGATTCCCAGAGCCCTCATGTTGTCGCCATCGGTCCTTTCCATCGACACAAGCCTAACCTAGTAGCTATGAGAGAGCACAAGTTGCGTTATGCGCTATATTTTTTCAAGCGAACACAAGAAATAGCAGATAAACGATCTCCAAGTTTCAGCTTTGGATCGCGAGAGTCTTCTGAATGCATGAGAGAGTGGGCAACTGCTATTTATGATTCGGACGCAGAGGTTCGTGGAAGCTACGCTGAAGATATGAGCAACATTGATCAGGAGGAACTAGCAGAAATAATGTTGGTCGATGGTTGCTTCTTATTAGAACTTTTCATCAGGTTTTACCTATACGTGCACAGGAAGTTAGAAGGCCCTGAATCTGATCCAATTCTAGAAAGTGCTTGGATGATTGCAGATCTACGGCACGATCTGGCATTGCTTGAAAACCAAATCCCCTTATTTATTATACTTGATTTGTATGATGCTATCAGTGGTCATCAGATTCTTGTCGACAATGATTTGCCTCCGCCACATGGCCTTGCTCTTCATTTCTTTCAACCTGTGAGCCTCAAGGCAAGCATTGTAACAGAGTGGAGACATGAGCACAAGCATTTGCTGGATATCTTGCACAAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

228

Amino Acids

26.38

Weight (kDa)

5.68

Isoelectric Point (pI)

58.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 9 - 227 6.3e-46 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 555
AccII CGCG 1 cut(s) 223
AciI CCGC 1 cut(s) 572
AclWI GGATC 2 cut(s) 226, 422
AcoI YGGCCR 1 cut(s) 7
AcuI CTGAAG 1 cut(s) 321
AfiI CCNNNNNNNGG 2 cut(s) 12, 578
AflII CTTAAG 1 cut(s) 19
AgsI TTSAA 3 cut(s) 169, 488, 602
AluBI AGCT 3 cut(s) 128, 213, 294
AluI AGCT 3 cut(s) 128, 213, 294
Alw21I GWGCWC 3 cut(s) 141, 402, 651
Alw26I GTCTC 1 cut(s) 634
Alw44I GTGCAC 1 cut(s) 398
AlwI GGATC 2 cut(s) 226, 422
AoxI GGCC 3 cut(s) 7, 415, 580
ApaLI GTGCAC 1 cut(s) 398
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 95, 416
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 3 cut(s) 141, 402, 651
BccI CCATC 3 cut(s) 98, 111, 347
BceAI ACGGC 1 cut(s) 482
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 634
BfaI CTAG 4 cut(s) 122, 332, 437, 682
BfmI CTRYAG 1 cut(s) 28
BfrI CTTAAG 1 cut(s) 19
BglII AGATCT 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 95
BmgT120I GGNCC 2 cut(s) 95, 416
BmsI GCATC 2 cut(s) 33, 517
BpiI GAAGAC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 599
BsaAI YACGTR 1 cut(s) 397
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 578
Bse3DI GCAATG 1 cut(s) 479
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 2 cut(s) 12, 578
BseMI GCAATG 1 cut(s) 479
BseSI GKGCMC 1 cut(s) 402
BseX3I CGGCCG 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 223
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 9, 417, 582
BsiEI CGRYCG 1 cut(s) 10
BsiHKAI GWGCWC 3 cut(s) 141, 402, 651
BsiSI CCGG 1 cut(s) 6
BslI CCNNNNNNNGG 2 cut(s) 12, 578
BsmAI GTCTC 1 cut(s) 634
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 3 cut(s) 9, 417, 582
Bsp1286I GDGCHC 4 cut(s) 77, 141, 402, 651
Bsp143I GATC 6 cut(s) 197, 218, 319, 427, 460, 472
Bsp68I TCGCGA 1 cut(s) 223
BspACI CCGC 1 cut(s) 572
BspANI GGCC 3 cut(s) 9, 417, 582
BspFNI CGCG 1 cut(s) 223
BspPI GGATC 2 cut(s) 226, 422
BspQI GCTCTTC 1 cut(s) 594
BspTI CTTAAG 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 479
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 6 cut(s) 197, 218, 319, 427, 460, 472
Bst6I CTCTTC 1 cut(s) 594
BstAFI CTTAAG 1 cut(s) 19
BstAPI GCANNNNNTGC 1 cut(s) 145
BstBAI YACGTR 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 622
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 223
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 6 cut(s) 200, 221, 322, 430, 463, 475
BstMAI GTCTC 1 cut(s) 634
BstMBI GATC 6 cut(s) 197, 218, 319, 427, 460, 472
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 145, 154
BstSFI CTRYAG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 402
BstUI CGCG 1 cut(s) 223
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BstZI CGGCCG 1 cut(s) 7
BsuRI GGCC 3 cut(s) 9, 417, 582
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 541
BtuMI TCGCGA 1 cut(s) 223
Cac8I GCNNGC 1 cut(s) 622
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 95, 416
CseI GACGC 1 cut(s) 284
CviAII CATG 4 cut(s) 80, 241, 578, 644
CviJI RGCY 9 cut(s) 9, 75, 115, 128, 213, 294, 417, 582, 612
CviKI_1 RGCY 9 cut(s) 9, 75, 115, 128, 213, 294, 417, 582, 612
DpnI GATC 6 cut(s) 199, 220, 321, 429, 462, 474
DpnII GATC 6 cut(s) 197, 218, 319, 427, 460, 472
EaeI YGGCCR 1 cut(s) 7
EagI CGGCCG 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
EciI GGCGGA 1 cut(s) 561
EclXI CGGCCG 1 cut(s) 7
Eco24I GRGCYC 1 cut(s) 77
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 95
Eco52I CGGCCG 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 416
EcoRV GATATC 1 cut(s) 667
EcoT22I ATGCAT 1 cut(s) 242
EcoT38I GRGCYC 1 cut(s) 77
FaeI CATG 4 cut(s) 83, 244, 581, 647
FatI CATG 4 cut(s) 79, 240, 577, 643
FbaI TGATCA 1 cut(s) 319
FblI GTMKAC 1 cut(s) 555
FokI GGATG 1 cut(s) 463
FriOI GRGCYC 1 cut(s) 77
FspBI CTAG 4 cut(s) 122, 332, 437, 682
GlaI GCGC 1 cut(s) 156
HaeIII GGCC 3 cut(s) 9, 417, 582
HapII CCGG 1 cut(s) 6
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 157
Hin1II CATG 4 cut(s) 83, 244, 581, 647
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 5 cut(s) 65, 227, 269, 422, 547
HpaII CCGG 1 cut(s) 6
Hpy166II GTNNAC 2 cut(s) 400, 556
Hpy188I TCNGA 4 cut(s) 235, 274, 427, 546
Hpy188III TCNNGA 3 cut(s) 222, 323, 437
Hpy8I GTNNAC 2 cut(s) 400, 556
HpyAV CCTTC 1 cut(s) 407
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 4 cut(s) 240, 400, 458, 673
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 154
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 4 cut(s) 83, 244, 581, 647
HspAI GCGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 6 cut(s) 197, 218, 319, 427, 460, 472
LguI GCTCTTC 1 cut(s) 594
LpnPI CCDG 9 cut(s) 19, 83, 308, 367, 388, 432, 461, 618, 647
LweI GCATC 2 cut(s) 33, 517
MaeI CTAG 4 cut(s) 122, 332, 437, 682
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 1 cut(s) 628
MalI GATC 6 cut(s) 199, 220, 321, 429, 462, 474
MboI GATC 6 cut(s) 197, 218, 319, 427, 460, 472
MboII GAAGA 3 cut(s) 222, 314, 581
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 4 cut(s) 77, 141, 402, 651
MluCI AATT 1 cut(s) 432
MlyI GAGTC 1 cut(s) 236
MnlI CCTC 6 cut(s) 55, 87, 274, 319, 579, 623
Mph1103I ATGCAT 1 cut(s) 242
MseI TTAA 1 cut(s) 20
MspCI CTTAAG 1 cut(s) 19
MspI CCGG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 242
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 2 cut(s) 145, 154
NdeII GATC 6 cut(s) 197, 218, 319, 427, 460, 472
NlaIII CATG 4 cut(s) 83, 244, 581, 647
NruI TCGCGA 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 594
PctI GAATGC 1 cut(s) 242
PfeI GAWTC 4 cut(s) 65, 269, 422, 547
PflFI GACNNNGTC 1 cut(s) 48
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 397
PspPI GGNCC 2 cut(s) 95, 416
PsuI RGATCY 1 cut(s) 460
PsyI GACNNNGTC 1 cut(s) 48
RruI TCGCGA 1 cut(s) 223
SalI GTCGAC 1 cut(s) 554
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 1 cut(s) 20
Sau3AI GATC 6 cut(s) 197, 218, 319, 427, 460, 472
Sau96I GGNCC 2 cut(s) 95, 416
SchI GAGTC 1 cut(s) 236
SduI GDGCHC 4 cut(s) 77, 141, 402, 651
SetI ASST 9 cut(s) 123, 130, 215, 285, 296, 386, 393, 399, 607
SfaNI GCATC 2 cut(s) 33, 517
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 1 cut(s) 95
SmlI CTYRAG 2 cut(s) 19, 614
SmoI CTYRAG 2 cut(s) 19, 614
Sse9I AATT 1 cut(s) 432
SsiI CCGC 1 cut(s) 572
SspMI CTAG 4 cut(s) 122, 332, 437, 682
TaiI ACGT 1 cut(s) 399
TaqI TCGA 4 cut(s) 57, 106, 351, 555
TaqII GACCGA 1 cut(s) 83
TasI AATT 1 cut(s) 432
TfiI GAWTC 4 cut(s) 65, 269, 422, 547
Tru1I TTAA 1 cut(s) 20
Tru9I TTAA 1 cut(s) 20
TscAI CASTG 1 cut(s) 541
TspDTI ATGAA 2 cut(s) 367, 581
TspRI CASTG 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 48
Vha464I CTTAAG 1 cut(s) 19
VneI GTGCAC 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 95
XbaI TCTAGA 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 555
XspI CTAG 4 cut(s) 122, 332, 437, 682
Zsp2I ATGCAT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.