Rh5BG129100

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000731
Physical Location & Seq
Forward (+)
12540588 .. 12542855
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG129100.1

Sequence Viewer

Length: 1554 bp
ATGGAGGAAATTAAATATGCTAAGATGAGCGATTCTGGAGATGAAATTCAGGAACCCAGGGCTGATCAACAGAAACGGAAAGATAGGTGGAAGAAAGGGATCATTGATAAGATCAACAGAACAAATTTGACAAAGTCGGTGACTATATATAACCCTCAAACCAAGAAAACAATAAAAGGGGAGATTTGCATCTATAGAGTTCCTGAAAAACTTCGTCGAGTGCAGGAGGATGCCTACCACCCTCGCGTCGTCTCGATTGGACCTTTCCATCGAGACACGCATAACCTTGAAGCCATGAAACAATATAAATGGTCTTACATGGTATCCCTTTTCCAGCAATCAAAAGATCAAGAAGACACTGAAGCTTCTTGGCGGTGTTTAGAAGCGTGCATCAATGTTATCTATGGATTAGACGAAGTGATTCGTCAATGCTATGCTGAGAAGATCAACTGCACAGAGGATGAGCTTGCAGAAATAATGTTGGTCGATGGTTGCTTCATTCTGGAACTTTTCCTCAGGTTTGACCGAAGCTTAAATTATATGAAGCTTGAAGATTTTGAAATTGATCCGGTACTAAAAAGTCCTTGGATGATCACAGCTCTTCAGCACGATCTTGGATTACTCGAAAACCAAATCCCATTTTTCATTCTTGAACTTCTGTATTATACTATCATGCCTCATGTCATCAATGTCATCAAGTGTGAACCACCGAAATCGGTGACTAGCCTTGCTCTTAAATTCTTTCCACCTATGAGCCAAAAGTCGATAGAAAAAGAGCAGGTGGCGGCAGAGTTTAAGCATTTGCTTGATCTTTTGCACCAATTCTACTACCTCCCAGCTGCAGCTACTGGAGCTCGTGACCTGTCTATCGAGTTCAACGATAGAGCGTCTCTGATTTCCGAGAACGAGCATCGTTCTGCTTCTGCTCCATGCAGTCGTGAACTTGATGATCAAGCAACCAATAAAAGTTCGGGATTCAGTTGTTGCGCTTCAGAGCTTCTCGAGTCTGGAATTGAGTTCCAAGTCAGGTCAAGTACCGAGGACCAACTGCTGGAAATAACTTTCAGTGAGGGAGTTATCAGAATTCCCCAACTGTACATTCATGAGACCTTCCGTTCACTCTTCAGGAATCTCATTGCTTTCGAGCAATGCAGTATTAGCAGTACCCATGGCGTTACAGCATACGCCTTCCTTATGGAGAGTCTCATCCGTTCCACGCAAGACATAAATCTGTTCACGAGGAAAAAGATCTTTAGGCCCAACTGGATCGGCAGTCAAGAATATTCTACTGCACTGAGAGACACTCTGAAGAAAGTCGTTACCAAGAACGATTCATATTTTGCTACATTATACAAGGAAGTGAATGACTACCGTAAGTCGTACCAGTGGAGCAGACTTCAAGTCTTTTTGAGGGTGCAATATCAAAGACAAAGAGGGATCCTTTGTACTACTTATTTCTCTACTACATGGAAAGTCATATCACTCATGGTTGCGGTGACCTTTCTCCTTCTCAGTGCCTTACAGACCTACTATACAATTAACCCTCACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

517

Amino Acids

60.32

Weight (kDa)

6.49

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 64 - 504 3e-99 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 769
Acc36I ACCTGC 1 cut(s) 769
AccB7I CCANNNNNTGG 1 cut(s) 1051
AccII CGCG 1 cut(s) 246
AciI CCGC 3 cut(s) 373, 785, 1493
AclWI GGATC 5 cut(s) 107, 560, 1274, 1432, 1445
AcsI RAATTY 4 cut(s) 45, 124, 737, 1083
AcuI CTGAAG 5 cut(s) 381, 587, 975, 1108, 1328
AfaI GTAC 6 cut(s) 573, 1036, 1097, 1165, 1382, 1447
AfiI CCNNNNNNNGG 1 cut(s) 1051
AgsI TTSAA 6 cut(s) 290, 551, 560, 653, 877, 1400
AhdI GACNNNNNGTC 1 cut(s) 1400
AjnI CCWGG 1 cut(s) 56
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
AluBI AGCT 9 cut(s) 365, 466, 531, 547, 599, 839, 845, 854, 997
AluI AGCT 9 cut(s) 365, 466, 531, 547, 599, 839, 845, 854, 997
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 6 cut(s) 256, 267, 894, 1100, 1208, 1293
AlwI GGATC 5 cut(s) 107, 560, 1274, 1432, 1445
AlwNI CAGNNNCTG 1 cut(s) 848
Ama87I CYCGRG 1 cut(s) 1001
AoxI GGCC 1 cut(s) 1256
ApeKI GCWGC 2 cut(s) 839, 842
ApoI RAATTY 4 cut(s) 45, 124, 737, 1083
Asp700I GAANNNNTTC 2 cut(s) 210, 420
AspLEI GCGC 1 cut(s) 989
AspS9I GGNCC 3 cut(s) 260, 1042, 1257
AsuHPI GGTGA 4 cut(s) 151, 730, 1507, 1538
AvaI CYCGRG 1 cut(s) 1001
AvaII GGWCC 2 cut(s) 260, 1042
AxyI CCTNAGG 1 cut(s) 515
BamHI GGATCC 1 cut(s) 1437
BanII GRGCYC 1 cut(s) 856
BauI CACGAG 2 cut(s) 855, 1237
BbsI GAAGAC 1 cut(s) 360
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 2 cut(s) 826, 854
BccI CCATC 2 cut(s) 276, 482
BciT130I CCWGG 1 cut(s) 58
BciVI GTATCC 1 cut(s) 334
BclI TGATCA 3 cut(s) 64, 591, 949
BcoDI GTCTC 6 cut(s) 256, 267, 894, 1100, 1208, 1293
BfaI CTAG 1 cut(s) 723
BfmI CTRYAG 2 cut(s) 193, 840
BfuAI ACCTGC 1 cut(s) 769
BfuI GTATCC 1 cut(s) 334
BglII AGATCT 1 cut(s) 1248
BisI GCNGC 3 cut(s) 786, 840, 843
BlsI GCNGC 3 cut(s) 787, 841, 844
Bme1390I CCNGG 1 cut(s) 58
Bme18I GGWCC 2 cut(s) 260, 1042
BmeRI GACNNNNNGTC 1 cut(s) 1400
BmeT110I CYCGRG 1 cut(s) 1001
BmgT120I GGNCC 3 cut(s) 260, 1042, 1257
BmiI GGNNCC 2 cut(s) 54, 1439
BmrFI CCNGG 1 cut(s) 58
BmsI GCATC 4 cut(s) 198, 220, 399, 919
BpiI GAAGAC 1 cut(s) 360
BplI GAGNNNNNCTC 2 cut(s) 1288, 1320
BpmI CTGGAG 2 cut(s) 57, 870
BsaBI GATNNNNATC 1 cut(s) 188
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 5 cut(s) 56, 57, 584, 1038, 1168
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 1 cut(s) 1051
Bse1I ACTGG 3 cut(s) 853, 1268, 1384
Bse21I CCTNAGG 1 cut(s) 515
Bse3DI GCAATG 2 cut(s) 1134, 1154
Bse8I GATNNNNATC 1 cut(s) 188
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 5 cut(s) 56, 57, 584, 1038, 1168
BseGI GGATG 4 cut(s) 235, 466, 594, 1206
BseJI GATNNNNATC 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 1051
BseMI GCAATG 2 cut(s) 1134, 1154
BseMII CTCAG 4 cut(s) 429, 529, 1286, 1525
BseNI ACTGG 3 cut(s) 853, 1268, 1384
BseXI GCAGC 2 cut(s) 826, 854
BseYI CCCAGC 1 cut(s) 835
BsgI GTGCAG 3 cut(s) 242, 436, 1275
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 1 cut(s) 1258
BsiHKAI GWGCWC 1 cut(s) 856
BsiHKCI CYCGRG 1 cut(s) 1001
BsiSI CCGG 1 cut(s) 569
BslI CCNNNNNNNGG 1 cut(s) 1051
BsmAI GTCTC 6 cut(s) 256, 267, 894, 1100, 1208, 1293
BsmBI CGTCTC 2 cut(s) 256, 894
BsnI GGCC 1 cut(s) 1258
Bso31I GGTCTC 1 cut(s) 1100
BsoBI CYCGRG 1 cut(s) 1001
Bsp1286I GDGCHC 1 cut(s) 856
Bsp1407I TGTACA 1 cut(s) 1095
Bsp19I CCATGG 1 cut(s) 1168
BspACI CCGC 3 cut(s) 373, 785, 1493
BspANI GGCC 1 cut(s) 1258
BspCNI CTCAG 4 cut(s) 430, 528, 1287, 1524
BspFNI CGCG 1 cut(s) 246
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 2 cut(s) 54, 1439
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 769
BspPI GGATC 5 cut(s) 107, 560, 1274, 1432, 1445
BspQI GCTCTTC 1 cut(s) 606
BspTNI GGTCTC 1 cut(s) 1100
BsrDI GCAATG 2 cut(s) 1134, 1154
BsrGI TGTACA 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 853, 1268, 1384
BssECI CCNNGG 5 cut(s) 56, 57, 584, 1038, 1168
BssSI CACGAG 2 cut(s) 855, 1237
BssT1I CCWWGG 2 cut(s) 584, 1168
Bst2BI CACGAG 2 cut(s) 855, 1237
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 2 cut(s) 1095, 1373
Bst6I CTCTTC 2 cut(s) 606, 1127
BstAUI TGTACA 1 cut(s) 1095
BstC8I GCNNGC 2 cut(s) 388, 468
BstDEI CTNAG 5 cut(s) 21, 438, 515, 1295, 1511
BstDSI CCRYGG 1 cut(s) 1168
BstEII GGTNACC 1 cut(s) 1495
BstF5I GGATG 4 cut(s) 235, 466, 594, 1206
BstFNI CGCG 1 cut(s) 246
BstHHI GCGC 1 cut(s) 989
BstMAI GTCTC 6 cut(s) 256, 267, 894, 1100, 1208, 1293
BstMWI GCNNNNNNNGC 2 cut(s) 851, 1158
BstNI CCWGG 1 cut(s) 58
BstPI GGTNACC 1 cut(s) 1495
BstSCI CCNGG 1 cut(s) 56
BstSFI CTRYAG 2 cut(s) 193, 840
BstUI CGCG 1 cut(s) 246
BstV1I GCAGC 2 cut(s) 826, 854
BstV2I GAAGAC 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 1248, 1437
BstYI RGATCY 2 cut(s) 1248, 1437
Bsu36I CCTNAGG 1 cut(s) 515
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 1 cut(s) 1258
BtgI CCRYGG 1 cut(s) 1168
BtsCI GGATG 4 cut(s) 235, 466, 594, 1206
BtsIMutI CAGTG 6 cut(s) 357, 1072, 1292, 1391, 1519, 1549
BveI ACCTGC 1 cut(s) 769
Cac8I GCNNGC 2 cut(s) 388, 468
CaiI CAGNNNCTG 1 cut(s) 848
CciI TCATGA 1 cut(s) 1102
CfoI GCGC 1 cut(s) 989
Cfr13I GGNCC 3 cut(s) 260, 1042, 1257
CseI GACGC 2 cut(s) 235, 876
Csp6I GTAC 6 cut(s) 572, 1035, 1096, 1164, 1381, 1446
CviAII CATG 9 cut(s) 295, 319, 673, 680, 930, 1103, 1169, 1467, 1486
CviQI GTAC 6 cut(s) 572, 1035, 1096, 1164, 1381, 1446
DdeI CTNAG 5 cut(s) 21, 438, 515, 1295, 1511
DriI GACNNNNNGTC 1 cut(s) 1400
Eam1104I CTCTTC 2 cut(s) 606, 1127
Eam1105I GACNNNNNGTC 1 cut(s) 1400
EarI CTCTTC 2 cut(s) 606, 1127
Ecl136II GAGCTC 1 cut(s) 854
Eco130I CCWWGG 2 cut(s) 584, 1168
Eco24I GRGCYC 1 cut(s) 856
Eco31I GGTCTC 1 cut(s) 1100
Eco47I GGWCC 2 cut(s) 260, 1042
Eco53kI GAGCTC 1 cut(s) 854
Eco57I CTGAAG 5 cut(s) 381, 587, 975, 1108, 1328
Eco81I CCTNAGG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 1001
Eco91I GGTNACC 1 cut(s) 1495
EcoICRI GAGCTC 1 cut(s) 854
EcoO65I GGTNACC 1 cut(s) 1495
EcoRI GAATTC 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 2 cut(s) 584, 1168
EcoT38I GRGCYC 1 cut(s) 856
ErhI CCWWGG 2 cut(s) 584, 1168
Esp3I CGTCTC 2 cut(s) 256, 894
FaeI CATG 9 cut(s) 298, 322, 676, 683, 933, 1106, 1172, 1470, 1489
FatI CATG 9 cut(s) 294, 318, 672, 679, 929, 1102, 1168, 1466, 1485
FbaI TGATCA 3 cut(s) 64, 591, 949
Fnu4HI GCNGC 3 cut(s) 786, 840, 843
FokI GGATG 4 cut(s) 242, 473, 601, 1193
FriOI GRGCYC 1 cut(s) 856
Fsp4HI GCNGC 3 cut(s) 786, 840, 843
FspBI CTAG 1 cut(s) 723
GlaI GCGC 1 cut(s) 988
GluI GCNGC 3 cut(s) 786, 840, 843
GsaI CCCAGC 1 cut(s) 839
GsuI CTGGAG 2 cut(s) 57, 870
HaeIII GGCC 1 cut(s) 1258
HapII CCGG 1 cut(s) 569
HgaI GACGC 2 cut(s) 235, 876
HhaI GCGC 1 cut(s) 989
Hin1II CATG 9 cut(s) 298, 322, 676, 683, 933, 1106, 1172, 1470, 1489
Hin6I GCGC 1 cut(s) 987
HinP1I GCGC 1 cut(s) 987
HindIII AAGCTT 3 cut(s) 363, 529, 545
HinfI GANTC 7 cut(s) 32, 421, 975, 1004, 1129, 1201, 1331
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 151, 730, 1507, 1538
Hpy166II GTNNAC 4 cut(s) 704, 941, 1118, 1236
Hpy188I TCNGA 5 cut(s) 894, 901, 994, 1082, 1308
Hpy8I GTNNAC 4 cut(s) 704, 941, 1118, 1236
Hpy99I CGWCG 2 cut(s) 219, 251
HpyAV CCTTC 3 cut(s) 1120, 1198, 1517
HpyCH4III ACNGT 2 cut(s) 1095, 1373
HpyF10VI GCNNNNNNNGC 2 cut(s) 851, 1158
HpyF3I CTNAG 5 cut(s) 21, 438, 515, 1295, 1511
Hsp92II CATG 9 cut(s) 298, 322, 676, 683, 933, 1106, 1172, 1470, 1489
HspAI GCGC 1 cut(s) 987
Ksp22I TGATCA 3 cut(s) 64, 591, 949
LguI GCTCTTC 1 cut(s) 606
LmnI GCTCC 3 cut(s) 851, 931, 1389
Lsp1109I GCAGC 2 cut(s) 826, 854
LweI GCATC 4 cut(s) 198, 220, 399, 919
MaeI CTAG 1 cut(s) 723
MaeIII GTNAC 6 cut(s) 139, 718, 857, 1174, 1318, 1495
MboII GAAGA 7 cut(s) 103, 365, 454, 563, 593, 1114, 1321
MflI RGATCY 2 cut(s) 1248, 1437
MhlI GDGCHC 1 cut(s) 856
MlyI GAGTC 2 cut(s) 1013, 1210
MroXI GAANNNNTTC 2 cut(s) 210, 420
MseI TTAA 5 cut(s) 12, 533, 735, 795, 1539
MspA1I CMGCKG 1 cut(s) 839
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 58
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 2 cut(s) 851, 1158
NcoI CCATGG 1 cut(s) 1168
NlaIII CATG 9 cut(s) 298, 322, 676, 683, 933, 1106, 1172, 1470, 1489
NlaIV GGNNCC 2 cut(s) 54, 1439
NmuCI GTSAC 4 cut(s) 139, 718, 857, 1495
PaeR7I CTCGAG 1 cut(s) 1001
PagI TCATGA 1 cut(s) 1102
PaqCI CACCTGC 1 cut(s) 769
PasI CCCWGGG 1 cut(s) 57
PciSI GCTCTTC 1 cut(s) 606
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PdmI GAANNNNTTC 2 cut(s) 210, 420
PfeI GAWTC 5 cut(s) 32, 421, 975, 1129, 1331
PflFI GACNNNGTC 1 cut(s) 133
PflMI CCANNNNNTGG 1 cut(s) 1051
PkrI GCNGC 3 cut(s) 787, 841, 844
PleI GAGTC 2 cut(s) 1012, 1209
PpsI GAGTC 2 cut(s) 1012, 1209
Psp124BI GAGCTC 1 cut(s) 856
Psp6I CCWGG 1 cut(s) 56
PspEI GGTNACC 1 cut(s) 1495
PspFI CCCAGC 1 cut(s) 835
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 2 cut(s) 54, 1439
PspPI GGNCC 3 cut(s) 260, 1042, 1257
PstI CTGCAG 1 cut(s) 844
PstNI CAGNNNCTG 1 cut(s) 848
PsuI RGATCY 2 cut(s) 1248, 1437
PsyI GACNNNGTC 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 839
RsaI GTAC 6 cut(s) 573, 1036, 1097, 1165, 1382, 1447
RsaNI GTAC 6 cut(s) 572, 1035, 1096, 1164, 1381, 1446
SacI GAGCTC 1 cut(s) 856
SapI GCTCTTC 1 cut(s) 606
SaqAI TTAA 5 cut(s) 12, 533, 735, 795, 1539
SatI GCNGC 3 cut(s) 786, 840, 843
Sau96I GGNCC 3 cut(s) 260, 1042, 1257
SchI GAGTC 2 cut(s) 1013, 1210
ScrFI CCNGG 1 cut(s) 58
SduI GDGCHC 1 cut(s) 856
SfaNI GCATC 4 cut(s) 198, 220, 399, 919
SfcI CTRYAG 2 cut(s) 193, 840
Sfr274I CTCGAG 1 cut(s) 1001
SinI GGWCC 2 cut(s) 260, 1042
SlaI CTCGAG 1 cut(s) 1001
SmlI CTYRAG 1 cut(s) 1001
SmoI CTYRAG 1 cut(s) 1001
SsiI CCGC 3 cut(s) 373, 785, 1493
SspI AATATT 1 cut(s) 1283
SspMI CTAG 1 cut(s) 723
SstI GAGCTC 1 cut(s) 856
StyD4I CCNGG 1 cut(s) 56
StyI CCWWGG 2 cut(s) 584, 1168
TaaI ACNGT 2 cut(s) 1095, 1373
TaqI TCGA 9 cut(s) 217, 254, 271, 486, 624, 764, 870, 1002, 1143
TaqII GACCGA 1 cut(s) 540
TatI WGTACW 2 cut(s) 1095, 1445
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 5 cut(s) 32, 421, 975, 1129, 1331
Tru1I TTAA 5 cut(s) 12, 533, 735, 795, 1539
Tru9I TTAA 5 cut(s) 12, 533, 735, 795, 1539
TscAI CASTG 5 cut(s) 364, 1072, 1299, 1391, 1519
TseFI GTSAC 4 cut(s) 139, 718, 857, 1495
TseI GCWGC 2 cut(s) 839, 842
Tsp45I GTSAC 4 cut(s) 139, 718, 857, 1495
TspDTI ATGAA 7 cut(s) 57, 311, 487, 557, 634, 1091, 1323
TspGWI ACGGA 3 cut(s) 91, 1103, 1199
TspRI CASTG 5 cut(s) 364, 1072, 1299, 1391, 1519
Tth111I GACNNNGTC 1 cut(s) 133
Van91I CCANNNNNTGG 1 cut(s) 1051
VpaK11BI GGWCC 2 cut(s) 260, 1042
XapI RAATTY 4 cut(s) 45, 124, 737, 1083
XhoI CTCGAG 1 cut(s) 1001
XmnI GAANNNNTTC 2 cut(s) 210, 420
XspI CTAG 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.