Rmu_sc0001775.1_g000014

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001775.1
OG0000731
Physical Location & Seq
Reverse (-)
43100 .. 44530
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001775.1_g000014.1.cds

Sequence Viewer

Length: 1431 bp
atgtatcgaaagaaacttaagtggaccaccgaaatcgaagaaaagctccacaatgtgcggccgtggaacttgggcatctacagagttccaaatagacttcgtcaagtcaatgaggatgcctacagccctcatgttgtctccatcggtcctttccatcgacacaagcctaacctagtagccatgagagagcacaagttgcgttatgcgctatatttctttaagcaagcacaagaaatagcacaaaaacgagctctaagtttcagctttggatcgcgcgagtcttccgaatgcatgagagagtgggcgactgctatttatgattcggacgcagaggttcgtggaagctacgctgaagatatgagcaacattgatcaggaggaactagcagaaataatgttggtcgatggttgcttcttattagaacttttcatcaggttttacctatacgtgcacgggaagttagaaggccctgaatctgatccaattctagaaagtgcttggatgattgcagatctacggcacgatctggcattgcttgaaaaccaaatccccttatttattatacatgatttgtatgatgctatcagtggtcatcagattcttgtcgacaatgatttgcctccgccacatggccttgctcttcatttctttcaacctgtgagcctcaaggcaagcattgtaacagagtggagacttgagtacaagcatttgatggatatcttgcacaagttctatttccttccgactaaaactacggacttgtctatgaggctcaatatggaattgatccctgcggagcagcgccttagggcagtccaaaagaataaagtatggggattcaattattgtgcctccgaacttttggaatctggagtcgagtttggcattggctccagccaggaccagttgctggacataactttccgtgagggagtaatcagaattccaccactgtttatccacgagacgacgagttcgttgttgaggaacctgattgcttatgagcaatgcagtcttagtagcacgcatgacgttacatcatatgcctgcctcatgaagagtctcatcgattcctcgcgggatagcaatctgcttcaggagaaggggataacaaaatatcaccactggattggaggtgaagatgaatatttgactcaaatattcagaagcatactagatgaagttgttgtcaaggaattccattttagaaccttgtgcgatgaagtgaatgaatactgtacgtcctggtttcatctgagaaaactcaaagtatttctgagggtgcggattcaaagatataggaagctccttttcagcacttatttttcttctccatggagtttcatatcattcatggcagcgctcaccattctccttctcacatctttgcagacatactattccatacacacagctcgtaatatcaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

476

Amino Acids

55.77

Weight (kDa)

6.29

Isoelectric Point (pI)

47.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 910
AccI GTMKAC 1 cut(s) 606
AccII CGCG 3 cut(s) 274, 276, 1078
AciI CCGC 5 cut(s) 58, 623, 794, 1078, 1285
AclWI GGATC 3 cut(s) 277, 473, 781
AcoI YGGCCR 1 cut(s) 59
AcsI RAATTY 2 cut(s) 942, 1196
AcuI CTGAAG 2 cut(s) 372, 1079
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 2 cut(s) 701, 1240
AfeI AGCGCT 1 cut(s) 1362
AfiI CCNNNNNNNGG 2 cut(s) 629, 910
AflII CTTAAG 1 cut(s) 17
AgsI TTSAA 4 cut(s) 539, 653, 841, 1292
AjnI CCWGG 2 cut(s) 897, 1244
AluBI AGCT 6 cut(s) 46, 251, 264, 345, 1306, 1415
AluI AGCT 6 cut(s) 46, 251, 264, 345, 1306, 1415
Alw21I GWGCWC 3 cut(s) 192, 253, 453
Alw26I GTCTC 4 cut(s) 142, 685, 959, 1067
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 3 cut(s) 277, 473, 781
AlwNI CAGNNNCTG 1 cut(s) 910
Aor51HI AGCGCT 1 cut(s) 1362
AoxI GGCC 3 cut(s) 59, 466, 631
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 2 cut(s) 799, 1358
ApoI RAATTY 2 cut(s) 942, 1196
ArsI GACNNNNNNTTYG 2 cut(s) 958, 990
AspLEI GCGC 4 cut(s) 208, 276, 804, 1363
AspS9I GGNCC 4 cut(s) 24, 146, 467, 901
AsuHPI GGTGA 3 cut(s) 1112, 1148, 1357
AvaII GGWCC 3 cut(s) 24, 146, 901
AxyI CCTNAGG 1 cut(s) 806
BaeGI GKGCMC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 253
BauI CACGAG 1 cut(s) 962
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 3 cut(s) 192, 253, 453
BbvI GCAGC 2 cut(s) 811, 1370
BccI CCATC 4 cut(s) 149, 162, 398, 706
BceAI ACGGC 2 cut(s) 46, 533
BciT130I CCWGG 2 cut(s) 899, 1246
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 4 cut(s) 142, 685, 959, 1067
BfaI CTAG 4 cut(s) 173, 383, 488, 1175
BfmI CTRYAG 2 cut(s) 79, 121
BfoI RGCGCY 2 cut(s) 805, 1364
BfrI CTTAAG 1 cut(s) 17
BglII AGATCT 1 cut(s) 511
BisI GCNGC 3 cut(s) 59, 800, 1359
BlsI GCNGC 3 cut(s) 60, 801, 1360
Bme1390I CCNGG 2 cut(s) 899, 1246
Bme18I GGWCC 3 cut(s) 24, 146, 901
BmgT120I GGNCC 4 cut(s) 24, 146, 467, 901
BmiI GGNNCC 2 cut(s) 892, 989
BmrFI CCNGG 2 cut(s) 899, 1246
BmsI GCATC 3 cut(s) 84, 106, 568
BpiI GAAGAC 1 cut(s) 273
BpmI CTGGAG 2 cut(s) 877, 891
BpuEI CTTGAG 2 cut(s) 650, 716
Bsa29I ATCGAT 1 cut(s) 1068
BsaAI YACGTR 1 cut(s) 448
BsaBI GATNNNNATC 2 cut(s) 716, 1086
BsaJI CCNNGG 2 cut(s) 62, 1334
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 910
Bse1I ACTGG 2 cut(s) 904, 1130
Bse21I CCTNAGG 1 cut(s) 806
Bse3DI GCAATG 2 cut(s) 530, 1013
Bse8I GATNNNNATC 2 cut(s) 716, 1086
BseBI CCWGG 2 cut(s) 899, 1246
BseCI ATCGAT 1 cut(s) 1068
BseDI CCNNGG 2 cut(s) 62, 1334
BseGI GGATG 2 cut(s) 121, 507
BseJI GATNNNNATC 2 cut(s) 716, 1086
BseLI CCNNNNNNNGG 2 cut(s) 629, 910
BseMI GCAATG 2 cut(s) 530, 1013
BseMII CTCAG 2 cut(s) 1247, 1268
BseNI ACTGG 2 cut(s) 904, 1130
BseSI GKGCMC 1 cut(s) 453
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 811, 1370
Bsh1236I CGCG 3 cut(s) 274, 276, 1078
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 3 cut(s) 61, 468, 633
BshVI ATCGAT 1 cut(s) 1068
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 3 cut(s) 192, 253, 453
BslI CCNNNNNNNGG 2 cut(s) 629, 910
BsmAI GTCTC 4 cut(s) 142, 685, 959, 1067
BsmBI CGTCTC 1 cut(s) 959
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 3 cut(s) 61, 468, 633
Bsp1286I GDGCHC 3 cut(s) 192, 253, 453
Bsp143I GATC 6 cut(s) 269, 370, 478, 511, 523, 786
Bsp19I CCATGG 1 cut(s) 1334
BspACI CCGC 5 cut(s) 58, 623, 794, 1078, 1285
BspANI GGCC 3 cut(s) 61, 468, 633
BspCNI CTCAG 2 cut(s) 1248, 1269
BspDI ATCGAT 1 cut(s) 1068
BspFNI CGCG 3 cut(s) 274, 276, 1078
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 2 cut(s) 892, 989
BspPI GGATC 3 cut(s) 277, 473, 781
BspQI GCTCTTC 1 cut(s) 645
BspTI CTTAAG 1 cut(s) 17
BsrDI GCAATG 2 cut(s) 530, 1013
BsrI ACTGG 2 cut(s) 904, 1130
BssECI CCNNGG 2 cut(s) 62, 1334
BssMI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
BssSI CACGAG 1 cut(s) 962
BssT1I CCWWGG 1 cut(s) 1334
Bst2BI CACGAG 1 cut(s) 962
Bst2UI CCWGG 2 cut(s) 899, 1246
Bst4CI ACNGT 2 cut(s) 954, 1238
Bst6I CTCTTC 2 cut(s) 645, 1052
BstAFI CTTAAG 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 196
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 4 cut(s) 225, 673, 1025, 1048
BstDEI CTNAG 5 cut(s) 254, 806, 1016, 1256, 1277
BstDSI CCRYGG 2 cut(s) 62, 1334
BstF5I GGATG 2 cut(s) 121, 507
BstFNI CGCG 3 cut(s) 274, 276, 1078
BstH2I RGCGCY 2 cut(s) 805, 1364
BstHHI GCGC 4 cut(s) 208, 276, 804, 1363
BstKTI GATC 6 cut(s) 272, 373, 481, 514, 526, 789
BstMAI GTCTC 4 cut(s) 142, 685, 959, 1067
BstMBI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 196, 205
BstNI CCWGG 2 cut(s) 899, 1246
BstSCI CCNGG 2 cut(s) 897, 1244
BstSFI CTRYAG 2 cut(s) 79, 121
BstSLI GKGCMC 1 cut(s) 453
BstUI CGCG 3 cut(s) 274, 276, 1078
BstV1I GCAGC 2 cut(s) 811, 1370
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 511
BstXI CCANNNNNNTGG 1 cut(s) 1130
BstYI RGATCY 1 cut(s) 511
BstZI CGGCCG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 1068
Bsu36I CCTNAGG 1 cut(s) 806
BsuRI GGCC 3 cut(s) 61, 468, 633
BsuTUI ATCGAT 1 cut(s) 1068
BtgI CCRYGG 2 cut(s) 62, 1334
BtgZI GCGATG 1 cut(s) 1233
BtsCI GGATG 2 cut(s) 121, 507
BtsIMutI CAGTG 3 cut(s) 592, 950, 1123
Cac8I GCNNGC 4 cut(s) 225, 673, 1025, 1048
CaiI CAGNNNCTG 1 cut(s) 910
CciI TCATGA 1 cut(s) 1053
CfoI GCGC 4 cut(s) 208, 276, 804, 1363
Cfr13I GGNCC 4 cut(s) 24, 146, 467, 901
ClaI ATCGAT 1 cut(s) 1068
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 700, 1239
CviAII CATG 9 cut(s) 131, 181, 292, 566, 629, 1028, 1054, 1335, 1354
CviQI GTAC 2 cut(s) 700, 1239
DdeI CTNAG 5 cut(s) 254, 806, 1016, 1256, 1277
DpnI GATC 6 cut(s) 271, 372, 480, 513, 525, 788
DpnII GATC 6 cut(s) 269, 370, 478, 511, 523, 786
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 59
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 2 cut(s) 645, 1052
EarI CTCTTC 2 cut(s) 645, 1052
EciI GGCGGA 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 251
EclXI CGGCCG 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 1334
Eco24I GRGCYC 1 cut(s) 253
Eco32I GATATC 1 cut(s) 718
Eco47I GGWCC 3 cut(s) 24, 146, 901
Eco47III AGCGCT 1 cut(s) 1362
Eco52I CGGCCG 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 2 cut(s) 372, 1079
Eco81I CCTNAGG 1 cut(s) 806
EcoICRI GAGCTC 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 467
EcoRI GAATTC 2 cut(s) 942, 1196
EcoRII CCWGG 2 cut(s) 897, 1244
EcoRV GATATC 1 cut(s) 718
EcoT14I CCWWGG 1 cut(s) 1334
EcoT22I ATGCAT 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 1334
Esp3I CGTCTC 1 cut(s) 959
FaeI CATG 9 cut(s) 134, 184, 295, 569, 632, 1031, 1057, 1338, 1357
FatI CATG 9 cut(s) 130, 180, 291, 565, 628, 1027, 1053, 1334, 1353
FauI CCCGC 1 cut(s) 1071
FauNDI CATATG 1 cut(s) 1042
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 3 cut(s) 59, 800, 1359
FokI GGATG 2 cut(s) 128, 514
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 3 cut(s) 59, 800, 1359
FspBI CTAG 4 cut(s) 173, 383, 488, 1175
GlaI GCGC 4 cut(s) 207, 275, 803, 1362
GluI GCNGC 3 cut(s) 59, 800, 1359
GsuI CTGGAG 2 cut(s) 877, 891
HaeII RGCGCY 2 cut(s) 805, 1364
HaeIII GGCC 3 cut(s) 61, 468, 633
HgaI GACGC 1 cut(s) 335
HhaI GCGC 4 cut(s) 208, 276, 804, 1363
Hin1II CATG 9 cut(s) 134, 184, 295, 569, 632, 1031, 1057, 1338, 1357
Hin6I GCGC 4 cut(s) 206, 274, 802, 1361
HinP1I GCGC 4 cut(s) 206, 274, 802, 1361
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HphI GGTGA 3 cut(s) 1112, 1148, 1357
Hpy166II GTNNAC 3 cut(s) 24, 451, 607
Hpy188III TCNNGA 5 cut(s) 374, 488, 870, 1054, 1097
Hpy8I GTNNAC 3 cut(s) 24, 451, 607
Hpy99I CGWCG 1 cut(s) 973
HpyAV CCTTC 4 cut(s) 458, 749, 1096, 1385
HpyCH4III ACNGT 2 cut(s) 954, 1238
HpyCH4IV ACGT 3 cut(s) 447, 1032, 1241
HpyCH4V TGCA 6 cut(s) 291, 451, 509, 724, 1011, 1390
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 205
HpyF3I CTNAG 5 cut(s) 254, 806, 1016, 1256, 1277
HpySE526I ACGT 3 cut(s) 447, 1032, 1241
Hsp92II CATG 9 cut(s) 134, 184, 295, 569, 632, 1031, 1057, 1338, 1357
HspAI GCGC 4 cut(s) 206, 274, 802, 1361
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 6 cut(s) 269, 370, 478, 511, 523, 786
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 4 cut(s) 51, 796, 896, 1311
Lsp1109I GCAGC 2 cut(s) 811, 1370
LweI GCATC 3 cut(s) 84, 106, 568
MaeI CTAG 4 cut(s) 173, 383, 488, 1175
MaeII ACGT 3 cut(s) 447, 1032, 1241
MaeIII GTNAC 2 cut(s) 679, 1033
MalI GATC 6 cut(s) 271, 372, 480, 513, 525, 788
MboI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
MboII GAAGA 7 cut(s) 50, 273, 365, 632, 1069, 1151, 1320
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 3 cut(s) 192, 253, 453
MluCI AATT 6 cut(s) 483, 782, 841, 942, 1196, 1426
MlyI GAGTC 4 cut(s) 287, 882, 1069, 1147
MmeI TCCRAC 1 cut(s) 767
Mph1103I ATGCAT 1 cut(s) 293
MseI TTAA 3 cut(s) 18, 219, 1429
MspCI CTTAAG 1 cut(s) 17
MspR9I CCNGG 2 cut(s) 899, 1246
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 2 cut(s) 899, 1246
MvnI CGCG 3 cut(s) 274, 276, 1078
MwoI GCNNNNNNNGC 2 cut(s) 196, 205
NcoI CCATGG 1 cut(s) 1334
NdeI CATATG 1 cut(s) 1042
NdeII GATC 6 cut(s) 269, 370, 478, 511, 523, 786
NlaIII CATG 9 cut(s) 134, 184, 295, 569, 632, 1031, 1057, 1338, 1357
NlaIV GGNNCC 2 cut(s) 892, 989
NsiI ATGCAT 1 cut(s) 293
PagI TCATGA 1 cut(s) 1053
PciSI GCTCTTC 1 cut(s) 645
PcsI WCGNNNNNNNCGW 1 cut(s) 974
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 7 cut(s) 320, 473, 598, 837, 866, 1070, 1288
PflFI GACNNNGTC 1 cut(s) 99
PflMI CCANNNNNTGG 1 cut(s) 910
PkrI GCNGC 3 cut(s) 60, 801, 1360
PleI GAGTC 4 cut(s) 286, 881, 1068, 1147
PpsI GAGTC 4 cut(s) 286, 881, 1068, 1147
Ppu21I YACGTR 1 cut(s) 448
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 897, 1244
PspGI CCWGG 2 cut(s) 897, 1244
PspN4I GGNNCC 2 cut(s) 892, 989
PspPI GGNCC 4 cut(s) 24, 146, 467, 901
PstNI CAGNNNCTG 1 cut(s) 910
PsuI RGATCY 1 cut(s) 511
PsyI GACNNNGTC 1 cut(s) 99
RsaI GTAC 2 cut(s) 701, 1240
RsaNI GTAC 2 cut(s) 700, 1239
SacI GAGCTC 1 cut(s) 253
SalI GTCGAC 1 cut(s) 605
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 3 cut(s) 18, 219, 1429
SatI GCNGC 3 cut(s) 59, 800, 1359
Sau3AI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
Sau96I GGNCC 4 cut(s) 24, 146, 467, 901
SchI GAGTC 4 cut(s) 287, 882, 1069, 1147
ScrFI CCNGG 2 cut(s) 899, 1246
SduI GDGCHC 3 cut(s) 192, 253, 453
SfaNI GCATC 3 cut(s) 84, 106, 568
SfcI CTRYAG 2 cut(s) 79, 121
SinI GGWCC 3 cut(s) 24, 146, 901
SmlI CTYRAG 3 cut(s) 17, 665, 695
SmoI CTYRAG 3 cut(s) 17, 665, 695
Sse9I AATT 6 cut(s) 483, 782, 841, 942, 1196, 1426
SsiI CCGC 5 cut(s) 58, 623, 794, 1078, 1285
SspI AATATT 2 cut(s) 1148, 1161
SspMI CTAG 4 cut(s) 173, 383, 488, 1175
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 2 cut(s) 897, 1244
StyI CCWWGG 1 cut(s) 1334
TaaI ACNGT 2 cut(s) 954, 1238
TaiI ACGT 3 cut(s) 450, 1035, 1244
TaqI TCGA 7 cut(s) 7, 36, 157, 402, 606, 876, 1068
TaqII GACCGA 1 cut(s) 134
TasI AATT 6 cut(s) 483, 782, 841, 942, 1196, 1426
TatI WGTACW 1 cut(s) 699
TauI GCSGC 1 cut(s) 61
TfiI GAWTC 7 cut(s) 320, 473, 598, 837, 866, 1070, 1288
Tru1I TTAA 3 cut(s) 18, 219, 1429
Tru9I TTAA 3 cut(s) 18, 219, 1429
TscAI CASTG 3 cut(s) 592, 957, 1130
TseI GCWGC 2 cut(s) 799, 1358
TspGWI ACGGA 2 cut(s) 770, 914
TspRI CASTG 3 cut(s) 592, 957, 1130
Tth111I GACNNNGTC 1 cut(s) 99
Van91I CCANNNNNTGG 1 cut(s) 910
Vha464I CTTAAG 1 cut(s) 17
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 3 cut(s) 24, 146, 901
XapI RAATTY 2 cut(s) 942, 1196
XbaI TCTAGA 1 cut(s) 487
XmiI GTMKAC 1 cut(s) 606
XspI CTAG 4 cut(s) 173, 383, 488, 1175
Zsp2I ATGCAT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.