Rmu_sc0003254.1_g000006

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003254.1
OG0000731
Physical Location & Seq
Forward (+)
18353 .. 19486
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003254.1_g000006.1.cds

Sequence Viewer

Length: 1134 bp
atgagctttggatcctatatacataaacagccagagagcgagataagctttacccaaaagatttatcgaaagaaacttaagtggaccaccgaaatcgaagaaaagctccacaatgtgcggccgtggaacttgggcatctacagagttccaaatagacttcgtcaagtcaatgaggatgcctacagccctcatgttgtctccatcggtcctttccatcgacacaagcctaacctagtagctatgagagagcacaagtcgcgttatgccctatacttctttaagcaagcacaagaaatagcacaaaaacgagctctaagtttcagctttggatcgcgcgagtcttccgaatgcatgagagagtgggcgactgttatttatgattcggacgcagaggttcgtggaagctacgctgaagatatgagcaacattgatcaggaggaactagcagaaataatgttggtcgatggttgcttcttattagaacttttcatcaggttttacctatacgtgcacgggaagttagaaggccctgaatctgatccaattctagaaagtgcttggatgattgcagatctacggcacgatctggcattgcttgaaaaccaaatccccttatttattatacatgatttgtatgatgctatcagtggtcatcagattcttgtcgacaatgatttgcctccgccacatggccttgctcttcatttctttcaacctgtgagcctcaaggcaagcattgtaacagagtggagacttgagtacaagcatttgatggatatcttgcacaagttctatttccttccgactaaaactacggacttgtctatgaggctcaatatggaattgatccctgcggagcagcgccttagggcagtccaaaagaataaagtatggggattcaattattgtgcctccgaacttttggaatctggagtcgagtttggcattggctccagccaggaccagttgctggacataactttccgtgagggagtaatcagaattccaccactgtttatccacgagacgacgagttcgttgttgaggaacctgattgcttatgagcaatgcagtcttagtagcacgcatgacgttacatcatatgcctgcctcatgaagagtctcatcgtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

377

Amino Acids

43.76

Weight (kDa)

5.9

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 970
AccI GTMKAC 1 cut(s) 666
AccII CGCG 3 cut(s) 259, 334, 336
AciI CCGC 3 cut(s) 118, 683, 854
AclWI GGATC 5 cut(s) 6, 19, 337, 533, 841
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 1 cut(s) 1002
AcuI CTGAAG 1 cut(s) 432
AdeI CACNNNGTG 1 cut(s) 115
AfaI GTAC 1 cut(s) 761
AfiI CCNNNNNNNGG 2 cut(s) 689, 970
AflII CTTAAG 1 cut(s) 77
AgsI TTSAA 3 cut(s) 599, 713, 901
AjnI CCWGG 1 cut(s) 957
AluBI AGCT 7 cut(s) 6, 48, 106, 239, 311, 324, 405
AluI AGCT 7 cut(s) 6, 48, 106, 239, 311, 324, 405
Alw21I GWGCWC 3 cut(s) 252, 313, 513
Alw26I GTCTC 4 cut(s) 202, 745, 1019, 1127
Alw44I GTGCAC 1 cut(s) 509
AlwI GGATC 5 cut(s) 6, 19, 337, 533, 841
AlwNI CAGNNNCTG 1 cut(s) 970
AoxI GGCC 3 cut(s) 119, 526, 691
ApaLI GTGCAC 1 cut(s) 509
ApeKI GCWGC 1 cut(s) 859
ApoI RAATTY 1 cut(s) 1002
ArsI GACNNNNNNTTYG 2 cut(s) 1018, 1050
AspLEI GCGC 2 cut(s) 336, 864
AspS9I GGNCC 4 cut(s) 84, 206, 527, 961
AvaII GGWCC 3 cut(s) 84, 206, 961
AxyI CCTNAGG 1 cut(s) 866
BaeGI GKGCMC 1 cut(s) 513
BamHI GGATCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 313
BauI CACGAG 1 cut(s) 1022
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 3 cut(s) 252, 313, 513
BbvI GCAGC 1 cut(s) 871
BccI CCATC 4 cut(s) 209, 222, 458, 766
BceAI ACGGC 2 cut(s) 106, 593
BciT130I CCWGG 1 cut(s) 959
BclI TGATCA 1 cut(s) 430
BcoDI GTCTC 4 cut(s) 202, 745, 1019, 1127
BfaI CTAG 3 cut(s) 233, 443, 548
BfmI CTRYAG 2 cut(s) 139, 181
BfoI RGCGCY 1 cut(s) 865
BfrI CTTAAG 1 cut(s) 77
BglII AGATCT 1 cut(s) 571
BisI GCNGC 2 cut(s) 119, 860
BlsI GCNGC 2 cut(s) 120, 861
Bme1390I CCNGG 1 cut(s) 959
Bme18I GGWCC 3 cut(s) 84, 206, 961
BmgT120I GGNCC 4 cut(s) 84, 206, 527, 961
BmiI GGNNCC 3 cut(s) 13, 952, 1049
BmrFI CCNGG 1 cut(s) 959
BmsI GCATC 3 cut(s) 144, 166, 628
BpiI GAAGAC 1 cut(s) 333
BpmI CTGGAG 2 cut(s) 937, 951
BpuEI CTTGAG 2 cut(s) 710, 776
BsaAI YACGTR 1 cut(s) 508
BsaBI GATNNNNATC 1 cut(s) 776
BsaJI CCNNGG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 2 cut(s) 689, 970
Bse1I ACTGG 1 cut(s) 964
Bse21I CCTNAGG 1 cut(s) 866
Bse3DI GCAATG 2 cut(s) 590, 1073
Bse8I GATNNNNATC 1 cut(s) 776
BseBI CCWGG 1 cut(s) 959
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 2 cut(s) 181, 567
BseJI GATNNNNATC 1 cut(s) 776
BseLI CCNNNNNNNGG 2 cut(s) 689, 970
BseMI GCAATG 2 cut(s) 590, 1073
BseNI ACTGG 1 cut(s) 964
BseSI GKGCMC 1 cut(s) 513
BseX3I CGGCCG 1 cut(s) 119
BseXI GCAGC 1 cut(s) 871
Bsh1236I CGCG 3 cut(s) 259, 334, 336
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 3 cut(s) 121, 528, 693
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 3 cut(s) 252, 313, 513
BslI CCNNNNNNNGG 2 cut(s) 689, 970
BsmAI GTCTC 4 cut(s) 202, 745, 1019, 1127
BsmBI CGTCTC 1 cut(s) 1019
BsmI GAATGC 1 cut(s) 353
BsnI GGCC 3 cut(s) 121, 528, 693
Bsp1286I GDGCHC 3 cut(s) 252, 313, 513
Bsp143I GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
BspACI CCGC 3 cut(s) 118, 683, 854
BspANI GGCC 3 cut(s) 121, 528, 693
BspFNI CGCG 3 cut(s) 259, 334, 336
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 3 cut(s) 13, 952, 1049
BspPI GGATC 5 cut(s) 6, 19, 337, 533, 841
BspQI GCTCTTC 1 cut(s) 705
BspTI CTTAAG 1 cut(s) 77
BsrDI GCAATG 2 cut(s) 590, 1073
BsrI ACTGG 1 cut(s) 964
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
BssSI CACGAG 1 cut(s) 1022
Bst2BI CACGAG 1 cut(s) 1022
Bst2UI CCWGG 1 cut(s) 959
Bst4CI ACNGT 2 cut(s) 370, 1014
Bst6I CTCTTC 2 cut(s) 705, 1112
BstAFI CTTAAG 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 508
BstC8I GCNNGC 4 cut(s) 285, 733, 1085, 1108
BstDEI CTNAG 3 cut(s) 314, 866, 1076
BstDSI CCRYGG 1 cut(s) 122
BstF5I GGATG 2 cut(s) 181, 567
BstFNI CGCG 3 cut(s) 259, 334, 336
BstH2I RGCGCY 1 cut(s) 865
BstHHI GCGC 2 cut(s) 336, 864
BstKTI GATC 7 cut(s) 14, 332, 433, 541, 574, 586, 849
BstMAI GTCTC 4 cut(s) 202, 745, 1019, 1127
BstMBI GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 2 cut(s) 45, 256
BstNI CCWGG 1 cut(s) 959
BstSCI CCNGG 1 cut(s) 957
BstSFI CTRYAG 2 cut(s) 139, 181
BstSLI GKGCMC 1 cut(s) 513
BstUI CGCG 3 cut(s) 259, 334, 336
BstV1I GCAGC 1 cut(s) 871
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 2 cut(s) 11, 571
BstYI RGATCY 2 cut(s) 11, 571
BstZI CGGCCG 1 cut(s) 119
Bsu36I CCTNAGG 1 cut(s) 866
BsuRI GGCC 3 cut(s) 121, 528, 693
BtgI CCRYGG 1 cut(s) 122
BtsCI GGATG 2 cut(s) 181, 567
BtsIMutI CAGTG 2 cut(s) 652, 1010
Cac8I GCNNGC 4 cut(s) 285, 733, 1085, 1108
CaiI CAGNNNCTG 1 cut(s) 970
CciI TCATGA 1 cut(s) 1113
CfoI GCGC 2 cut(s) 336, 864
Cfr13I GGNCC 4 cut(s) 84, 206, 527, 961
CseI GACGC 1 cut(s) 395
Csp6I GTAC 1 cut(s) 760
CviAII CATG 6 cut(s) 191, 352, 626, 689, 1088, 1114
CviQI GTAC 1 cut(s) 760
DdeI CTNAG 3 cut(s) 314, 866, 1076
DpnI GATC 7 cut(s) 13, 331, 432, 540, 573, 585, 848
DpnII GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
DraIII CACNNNGTG 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 119
EagI CGGCCG 1 cut(s) 119
Eam1104I CTCTTC 2 cut(s) 705, 1112
EarI CTCTTC 2 cut(s) 705, 1112
EciI GGCGGA 1 cut(s) 672
Ecl136II GAGCTC 1 cut(s) 311
EclXI CGGCCG 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 313
Eco32I GATATC 1 cut(s) 778
Eco47I GGWCC 3 cut(s) 84, 206, 961
Eco52I CGGCCG 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 432
Eco81I CCTNAGG 1 cut(s) 866
EcoICRI GAGCTC 1 cut(s) 311
EcoO109I RGGNCCY 1 cut(s) 527
EcoRI GAATTC 1 cut(s) 1002
EcoRII CCWGG 1 cut(s) 957
EcoRV GATATC 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 353
EcoT38I GRGCYC 1 cut(s) 313
Esp3I CGTCTC 1 cut(s) 1019
FaeI CATG 6 cut(s) 194, 355, 629, 692, 1091, 1117
FatI CATG 6 cut(s) 190, 351, 625, 688, 1087, 1113
FauNDI CATATG 1 cut(s) 1102
FbaI TGATCA 1 cut(s) 430
FblI GTMKAC 1 cut(s) 666
Fnu4HI GCNGC 2 cut(s) 119, 860
FokI GGATG 2 cut(s) 188, 574
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 2 cut(s) 119, 860
FspBI CTAG 3 cut(s) 233, 443, 548
GlaI GCGC 2 cut(s) 335, 863
GluI GCNGC 2 cut(s) 119, 860
GsuI CTGGAG 2 cut(s) 937, 951
HaeII RGCGCY 1 cut(s) 865
HaeIII GGCC 3 cut(s) 121, 528, 693
HgaI GACGC 1 cut(s) 395
HhaI GCGC 2 cut(s) 336, 864
Hin1II CATG 6 cut(s) 194, 355, 629, 692, 1091, 1117
Hin6I GCGC 2 cut(s) 334, 862
HinP1I GCGC 2 cut(s) 334, 862
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 46
HinfI GANTC 8 cut(s) 338, 380, 533, 658, 897, 926, 933, 1120
Hpy166II GTNNAC 3 cut(s) 84, 511, 667
Hpy188I TCNGA 7 cut(s) 346, 385, 538, 657, 804, 916, 1001
Hpy188III TCNNGA 4 cut(s) 434, 548, 930, 1114
Hpy8I GTNNAC 3 cut(s) 84, 511, 667
Hpy99I CGWCG 1 cut(s) 1033
HpyAV CCTTC 2 cut(s) 518, 809
HpyCH4III ACNGT 2 cut(s) 370, 1014
HpyCH4IV ACGT 2 cut(s) 507, 1092
HpyCH4V TGCA 5 cut(s) 351, 511, 569, 784, 1071
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 256
HpyF3I CTNAG 3 cut(s) 314, 866, 1076
HpySE526I ACGT 2 cut(s) 507, 1092
Hsp92II CATG 6 cut(s) 194, 355, 629, 692, 1091, 1117
HspAI GCGC 2 cut(s) 334, 862
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 3 cut(s) 111, 856, 956
Lsp1109I GCAGC 1 cut(s) 871
LweI GCATC 3 cut(s) 144, 166, 628
MaeI CTAG 3 cut(s) 233, 443, 548
MaeII ACGT 2 cut(s) 507, 1092
MaeIII GTNAC 2 cut(s) 739, 1093
MalI GATC 7 cut(s) 13, 331, 432, 540, 573, 585, 848
MboI GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
MboII GAAGA 5 cut(s) 110, 333, 425, 692, 1129
MflI RGATCY 2 cut(s) 11, 571
MhlI GDGCHC 3 cut(s) 252, 313, 513
MluCI AATT 4 cut(s) 543, 842, 901, 1002
MlyI GAGTC 3 cut(s) 347, 942, 1129
MmeI TCCRAC 1 cut(s) 827
Mph1103I ATGCAT 1 cut(s) 353
MseI TTAA 2 cut(s) 78, 279
MspCI CTTAAG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 959
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 1 cut(s) 959
MvnI CGCG 3 cut(s) 259, 334, 336
MwoI GCNNNNNNNGC 2 cut(s) 45, 256
NdeI CATATG 1 cut(s) 1102
NdeII GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
NlaIII CATG 6 cut(s) 194, 355, 629, 692, 1091, 1117
NlaIV GGNNCC 3 cut(s) 13, 952, 1049
NsiI ATGCAT 1 cut(s) 353
PagI TCATGA 1 cut(s) 1113
PciSI GCTCTTC 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 1034
PctI GAATGC 1 cut(s) 353
PfeI GAWTC 5 cut(s) 380, 533, 658, 897, 926
PflFI GACNNNGTC 1 cut(s) 159
PflMI CCANNNNNTGG 1 cut(s) 970
PkrI GCNGC 2 cut(s) 120, 861
PleI GAGTC 3 cut(s) 346, 941, 1128
PpsI GAGTC 3 cut(s) 346, 941, 1128
Ppu21I YACGTR 1 cut(s) 508
Psp124BI GAGCTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 957
PspGI CCWGG 1 cut(s) 957
PspN4I GGNNCC 3 cut(s) 13, 952, 1049
PspPI GGNCC 4 cut(s) 84, 206, 527, 961
PstNI CAGNNNCTG 1 cut(s) 970
PsuI RGATCY 2 cut(s) 11, 571
PsyI GACNNNGTC 1 cut(s) 159
RsaI GTAC 1 cut(s) 761
RsaNI GTAC 1 cut(s) 760
SacI GAGCTC 1 cut(s) 313
SalI GTCGAC 1 cut(s) 665
SapI GCTCTTC 1 cut(s) 705
SaqAI TTAA 2 cut(s) 78, 279
SatI GCNGC 2 cut(s) 119, 860
Sau3AI GATC 7 cut(s) 11, 329, 430, 538, 571, 583, 846
Sau96I GGNCC 4 cut(s) 84, 206, 527, 961
SchI GAGTC 3 cut(s) 347, 942, 1129
ScrFI CCNGG 1 cut(s) 959
SduI GDGCHC 3 cut(s) 252, 313, 513
SfaNI GCATC 3 cut(s) 144, 166, 628
SfcI CTRYAG 2 cut(s) 139, 181
SinI GGWCC 3 cut(s) 84, 206, 961
SmlI CTYRAG 3 cut(s) 77, 725, 755
SmoI CTYRAG 3 cut(s) 77, 725, 755
Sse9I AATT 4 cut(s) 543, 842, 901, 1002
SsiI CCGC 3 cut(s) 118, 683, 854
SspMI CTAG 3 cut(s) 233, 443, 548
SstI GAGCTC 1 cut(s) 313
StyD4I CCNGG 1 cut(s) 957
TaaI ACNGT 2 cut(s) 370, 1014
TaiI ACGT 2 cut(s) 510, 1095
TaqI TCGA 6 cut(s) 67, 96, 217, 462, 666, 936
TaqII GACCGA 1 cut(s) 194
TasI AATT 4 cut(s) 543, 842, 901, 1002
TatI WGTACW 1 cut(s) 759
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 5 cut(s) 380, 533, 658, 897, 926
Tru1I TTAA 2 cut(s) 78, 279
Tru9I TTAA 2 cut(s) 78, 279
TscAI CASTG 2 cut(s) 652, 1017
TseI GCWGC 1 cut(s) 859
TspDTI ATGAA 3 cut(s) 478, 692, 1130
TspGWI ACGGA 2 cut(s) 830, 974
TspRI CASTG 2 cut(s) 652, 1017
Tth111I GACNNNGTC 1 cut(s) 159
Van91I CCANNNNNTGG 1 cut(s) 970
Vha464I CTTAAG 1 cut(s) 77
VneI GTGCAC 1 cut(s) 509
VpaK11BI GGWCC 3 cut(s) 84, 206, 961
XapI RAATTY 1 cut(s) 1002
XbaI TCTAGA 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 666
XspI CTAG 3 cut(s) 233, 443, 548
Zsp2I ATGCAT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.