Rh5AG131300

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000731
Physical Location & Seq
Forward (+)
13211489 .. 13213704
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG131300.1

Sequence Viewer

Length: 1557 bp
ATGATGGAGGAAATTAAATATGCTAAGATGAGCGATTCTGGAGATGAAATTCAGGAACCCAGGGGAGATCAACAGAAACGGAAAGATAGGTGGAAGAAAGGGATCATTGATAAGATCAACAGAACAAATTTGACAAAGTCGGTGACTATATATAACCCTCAAACCAAGAAAACAATAAAAGGGGAGATTTGCATCTATAGAGTTCCTGAAAAACTTCGTCAAGTGCAGGAGGATGCCTACCACCCTCGCGTTGTCTCGATTGGACCTTTCCATCGAGACACGCATAACCTTGAAGCCATGAAACAATATAAATGGTCTTACATGGTATCCCTTTTCCAGCAATCAAAAGATCAAGAAGACACTGAAGCTTCTTGGCGGTGTTTAGAAGCGTGCATCAATGTTATCTATGGATTAGACGAAGTGATTCGTCAATGCTATGCTGAGAAGATCAACTGCACAGAGGATGAGCTTGCAGAAATAATGTTGGTCGATGGTTGCTTCATTCTGGAACTTTTCCTAAGGTTTGACCGAAGCTTAAATTATATGAAGCGTGAAGATTTTGAAATTGATCCAGTACTGAAAAGTCCTTGGATGATCACAGCTCTTCAGCACGATCTTGGATTACTCGAAAACCAAATCCCATTTTTCATTCTTGAACTTCTGTATTATACTATCATGCCTCATGTCATCAATGTCATCAAGTGTGAACCACCGAAATCGGTGACTAGCCTTGCTCTTAAATTCTTTCCACCTATGAGCCAAAAGTCGATAGAAAAAGAGCAGGTGGCGGTAGAGTTTAAGCATTTGCTTGATCTTTTGCACCGATTCTACTACCTCCCAGCTGCAGCCACTGGAGCTCGTGACCTGTCTATCGAGTTCAACGATAGAGCGTCTCTGATTTCCGAGAACGAGCATCGTTCTGCTTCTCCACCATGCAGTCGTGAACTTGATGATCAAGCAACCAATAAAAGTTCGGGATTCAGTTGTTGCGCTTCAGAACTTCTCGAGTCTGGAACTGAGTTCCAAGTCAGGTCAAGTACCGAGGACCAACTGCTGGAAATAACTTTCAGTGAGGGAGTTATCAGAATTCCCCAATTGTACATTCATGAGACCTCCCGTTCACTCATCAGAAATCTCATTGCTTTCGAGCAATGCAGTATTCGCAGTACCCATGGCGTTACAGCATACGCCTTCCTTATGGAGAGTCTCATCCGTTCCACGCAAGACATAAATCTGCTCACGAGGAAAAAGATCTTAAGGCACAACTGGATCGGCAATCAAGAATATTCTACTGCACTGAGAGACACTCTGAAGAAAGTCGTTACCAAGAACGATTCATATTTTGCTACATTATACAAGGAAGTCAATGACTACCGTAAGTCGTACCAGTGGAGCAGACTTCAAGTCTTTTTGAGGGTGCAATATCAAAGACAAAGAGGGATCCTTTGTACCACTTATTTCTCTACTACATGGAAGGTCGTATCATTCCTGGTTGCGGTGACCTTTCTCCTTCTCAGTGCCATACAGACGTACTATACAATTAATCCTCACCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

518

Amino Acids

60.5

Weight (kDa)

6.9

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 65 - 505 1.8e-99 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 772
Acc36I ACCTGC 1 cut(s) 772
AccB7I CCANNNNNTGG 1 cut(s) 1054
AccII CGCG 1 cut(s) 249
AciI CCGC 3 cut(s) 376, 788, 1496
AclWI GGATC 5 cut(s) 110, 563, 1277, 1435, 1448
AcsI RAATTY 4 cut(s) 48, 127, 740, 1086
AcuI CTGAAG 4 cut(s) 384, 590, 978, 1331
AfaI GTAC 7 cut(s) 576, 1039, 1100, 1168, 1385, 1450, 1532
AfiI CCNNNNNNNGG 2 cut(s) 1054, 1495
AflII CTTAAG 1 cut(s) 1255
AgsI TTSAA 5 cut(s) 293, 563, 656, 880, 1403
AhdI GACNNNNNGTC 1 cut(s) 1403
AjnI CCWGG 2 cut(s) 59, 1488
AjuI GAANNNNNNNTTGG 2 cut(s) 1086, 1118
AluBI AGCT 6 cut(s) 368, 469, 534, 602, 842, 857
AluI AGCT 6 cut(s) 368, 469, 534, 602, 842, 857
Alw21I GWGCWC 1 cut(s) 859
Alw26I GTCTC 6 cut(s) 259, 270, 897, 1103, 1211, 1296
AlwI GGATC 5 cut(s) 110, 563, 1277, 1435, 1448
AlwNI CAGNNNCTG 1 cut(s) 851
Ama87I CYCGRG 1 cut(s) 1004
ApeKI GCWGC 2 cut(s) 842, 845
ApoI RAATTY 4 cut(s) 48, 127, 740, 1086
AseI ATTAAT 1 cut(s) 1542
Asp700I GAANNNNTTC 2 cut(s) 213, 423
AspLEI GCGC 1 cut(s) 992
AspS9I GGNCC 2 cut(s) 263, 1045
AsuHPI GGTGA 4 cut(s) 154, 733, 1510, 1541
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 2 cut(s) 263, 1045
AxyI CCTNAGG 1 cut(s) 518
BamHI GGATCC 1 cut(s) 1440
BanII GRGCYC 1 cut(s) 859
BauI CACGAG 2 cut(s) 858, 1240
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 859
BbvI GCAGC 2 cut(s) 829, 857
BccI CCATC 2 cut(s) 279, 485
BciT130I CCWGG 2 cut(s) 61, 1490
BciVI GTATCC 1 cut(s) 337
BclI TGATCA 2 cut(s) 594, 952
BcoDI GTCTC 6 cut(s) 259, 270, 897, 1103, 1211, 1296
BfaI CTAG 1 cut(s) 726
BfmI CTRYAG 2 cut(s) 196, 843
BfrI CTTAAG 1 cut(s) 1255
BfuAI ACCTGC 1 cut(s) 772
BfuI GTATCC 1 cut(s) 337
BglII AGATCT 1 cut(s) 1251
BisI GCNGC 2 cut(s) 843, 846
BlsI GCNGC 2 cut(s) 844, 847
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 2 cut(s) 61, 1490
Bme18I GGWCC 2 cut(s) 263, 1045
BmeRI GACNNNNNGTC 1 cut(s) 1403
BmeT110I CYCGRG 1 cut(s) 1004
BmgT120I GGNCC 2 cut(s) 263, 1045
BmiI GGNNCC 2 cut(s) 57, 1442
BmrFI CCNGG 2 cut(s) 61, 1490
BmsI GCATC 4 cut(s) 201, 223, 402, 922
BpiI GAAGAC 1 cut(s) 363
BplI GAGNNNNNCTC 2 cut(s) 1291, 1323
BpmI CTGGAG 2 cut(s) 60, 873
BsaBI GATNNNNATC 1 cut(s) 191
BsaI GGTCTC 1 cut(s) 1103
BsaJI CCNNGG 5 cut(s) 59, 60, 587, 1041, 1171
Bsc4I CCNNNNNNNGG 2 cut(s) 1054, 1495
Bse1I ACTGG 4 cut(s) 572, 856, 1271, 1387
Bse21I CCTNAGG 1 cut(s) 518
Bse3DI GCAATG 2 cut(s) 1137, 1157
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 2 cut(s) 61, 1490
BseDI CCNNGG 5 cut(s) 59, 60, 587, 1041, 1171
BseGI GGATG 4 cut(s) 238, 469, 597, 1209
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 2 cut(s) 1054, 1495
BseMI GCAATG 2 cut(s) 1137, 1157
BseMII CTCAG 4 cut(s) 432, 1008, 1289, 1528
BseNI ACTGG 4 cut(s) 572, 856, 1271, 1387
BseXI GCAGC 2 cut(s) 829, 857
BseYI CCCAGC 1 cut(s) 838
BsgI GTGCAG 3 cut(s) 245, 439, 1278
Bsh1236I CGCG 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 859
BsiHKCI CYCGRG 1 cut(s) 1004
BslI CCNNNNNNNGG 2 cut(s) 1054, 1495
BsmAI GTCTC 6 cut(s) 259, 270, 897, 1103, 1211, 1296
BsmBI CGTCTC 1 cut(s) 897
Bso31I GGTCTC 1 cut(s) 1103
BsoBI CYCGRG 1 cut(s) 1004
Bsp1286I GDGCHC 1 cut(s) 859
Bsp1407I TGTACA 1 cut(s) 1098
Bsp19I CCATGG 1 cut(s) 1171
BspACI CCGC 3 cut(s) 376, 788, 1496
BspCNI CTCAG 4 cut(s) 433, 1009, 1290, 1527
BspFNI CGCG 1 cut(s) 249
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 2 cut(s) 57, 1442
BspMAI CTGCAG 1 cut(s) 847
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 5 cut(s) 110, 563, 1277, 1435, 1448
BspQI GCTCTTC 1 cut(s) 609
BspTI CTTAAG 1 cut(s) 1255
BspTNI GGTCTC 1 cut(s) 1103
BsrDI GCAATG 2 cut(s) 1137, 1157
BsrGI TGTACA 1 cut(s) 1098
BsrI ACTGG 4 cut(s) 572, 856, 1271, 1387
BssECI CCNNGG 5 cut(s) 59, 60, 587, 1041, 1171
BssSI CACGAG 2 cut(s) 858, 1240
BssT1I CCWWGG 2 cut(s) 587, 1171
Bst2BI CACGAG 2 cut(s) 858, 1240
Bst2UI CCWGG 2 cut(s) 61, 1490
Bst4CI ACNGT 1 cut(s) 1376
Bst6I CTCTTC 1 cut(s) 609
BstAFI CTTAAG 1 cut(s) 1255
BstAUI TGTACA 1 cut(s) 1098
BstC8I GCNNGC 2 cut(s) 391, 471
BstDEI CTNAG 6 cut(s) 24, 441, 518, 1017, 1298, 1514
BstDSI CCRYGG 1 cut(s) 1171
BstEII GGTNACC 1 cut(s) 1498
BstF5I GGATG 4 cut(s) 238, 469, 597, 1209
BstFNI CGCG 1 cut(s) 249
BstHHI GCGC 1 cut(s) 992
BstMAI GTCTC 6 cut(s) 259, 270, 897, 1103, 1211, 1296
BstMWI GCNNNNNNNGC 2 cut(s) 854, 1161
BstNI CCWGG 2 cut(s) 61, 1490
BstPI GGTNACC 1 cut(s) 1498
BstSCI CCNGG 2 cut(s) 59, 1488
BstSFI CTRYAG 2 cut(s) 196, 843
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 2 cut(s) 829, 857
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 2 cut(s) 1251, 1440
BstYI RGATCY 2 cut(s) 1251, 1440
Bsu36I CCTNAGG 1 cut(s) 518
BsuI GTATCC 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 1171
BtsCI GGATG 4 cut(s) 238, 469, 597, 1209
BtsIMutI CAGTG 7 cut(s) 360, 849, 1075, 1295, 1394, 1522, 1552
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 2 cut(s) 391, 471
CaiI CAGNNNCTG 1 cut(s) 851
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 1 cut(s) 992
Cfr13I GGNCC 2 cut(s) 263, 1045
CseI GACGC 1 cut(s) 879
Csp6I GTAC 7 cut(s) 575, 1038, 1099, 1167, 1384, 1449, 1531
CviAII CATG 8 cut(s) 298, 322, 676, 683, 933, 1106, 1172, 1470
CviQI GTAC 7 cut(s) 575, 1038, 1099, 1167, 1384, 1449, 1531
DdeI CTNAG 6 cut(s) 24, 441, 518, 1017, 1298, 1514
DriI GACNNNNNGTC 1 cut(s) 1403
Eam1104I CTCTTC 1 cut(s) 609
Eam1105I GACNNNNNGTC 1 cut(s) 1403
EarI CTCTTC 1 cut(s) 609
Ecl136II GAGCTC 1 cut(s) 857
Eco130I CCWWGG 2 cut(s) 587, 1171
Eco24I GRGCYC 1 cut(s) 859
Eco31I GGTCTC 1 cut(s) 1103
Eco47I GGWCC 2 cut(s) 263, 1045
Eco53kI GAGCTC 1 cut(s) 857
Eco57I CTGAAG 4 cut(s) 384, 590, 978, 1331
Eco81I CCTNAGG 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 1004
Eco91I GGTNACC 1 cut(s) 1498
EcoICRI GAGCTC 1 cut(s) 857
EcoO65I GGTNACC 1 cut(s) 1498
EcoRI GAATTC 1 cut(s) 1086
EcoRII CCWGG 2 cut(s) 59, 1488
EcoT14I CCWWGG 2 cut(s) 587, 1171
EcoT38I GRGCYC 1 cut(s) 859
ErhI CCWWGG 2 cut(s) 587, 1171
Esp3I CGTCTC 1 cut(s) 897
FaeI CATG 8 cut(s) 301, 325, 679, 686, 936, 1109, 1175, 1473
FatI CATG 8 cut(s) 297, 321, 675, 682, 932, 1105, 1171, 1469
FbaI TGATCA 2 cut(s) 594, 952
Fnu4HI GCNGC 2 cut(s) 843, 846
FokI GGATG 4 cut(s) 245, 476, 604, 1196
FriOI GRGCYC 1 cut(s) 859
Fsp4HI GCNGC 2 cut(s) 843, 846
FspBI CTAG 1 cut(s) 726
GlaI GCGC 1 cut(s) 991
GluI GCNGC 2 cut(s) 843, 846
GsaI CCCAGC 1 cut(s) 842
GsuI CTGGAG 2 cut(s) 60, 873
HgaI GACGC 1 cut(s) 879
HhaI GCGC 1 cut(s) 992
Hin1II CATG 8 cut(s) 301, 325, 679, 686, 936, 1109, 1175, 1473
Hin6I GCGC 1 cut(s) 990
HinP1I GCGC 1 cut(s) 990
HindIII AAGCTT 2 cut(s) 366, 532
HinfI GANTC 7 cut(s) 35, 424, 825, 978, 1007, 1204, 1334
HphI GGTGA 4 cut(s) 154, 733, 1510, 1541
Hpy166II GTNNAC 3 cut(s) 707, 944, 1121
Hpy188I TCNGA 6 cut(s) 897, 904, 997, 1085, 1130, 1311
Hpy8I GTNNAC 3 cut(s) 707, 944, 1121
HpyAV CCTTC 3 cut(s) 1201, 1468, 1520
HpyCH4III ACNGT 1 cut(s) 1376
HpyCH4IV ACGT 1 cut(s) 1529
HpyF10VI GCNNNNNNNGC 2 cut(s) 854, 1161
HpyF3I CTNAG 6 cut(s) 24, 441, 518, 1017, 1298, 1514
HpySE526I ACGT 1 cut(s) 1529
Hsp92II CATG 8 cut(s) 301, 325, 679, 686, 936, 1109, 1175, 1473
HspAI GCGC 1 cut(s) 990
Ksp22I TGATCA 2 cut(s) 594, 952
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 2 cut(s) 854, 1392
Lsp1109I GCAGC 2 cut(s) 829, 857
LweI GCATC 4 cut(s) 201, 223, 402, 922
MaeI CTAG 1 cut(s) 726
MaeII ACGT 1 cut(s) 1529
MaeIII GTNAC 6 cut(s) 142, 721, 860, 1177, 1321, 1498
MboII GAAGA 6 cut(s) 106, 368, 457, 566, 596, 1324
MfeI CAATTG 1 cut(s) 1094
MflI RGATCY 2 cut(s) 1251, 1440
MhlI GDGCHC 1 cut(s) 859
MluCI AATT 9 cut(s) 12, 48, 127, 538, 564, 740, 1086, 1094, 1539
MlyI GAGTC 2 cut(s) 1016, 1213
MroXI GAANNNNTTC 2 cut(s) 213, 423
MseI TTAA 6 cut(s) 15, 536, 738, 798, 1256, 1542
MspA1I CMGCKG 1 cut(s) 842
MspCI CTTAAG 1 cut(s) 1255
MspR9I CCNGG 2 cut(s) 61, 1490
MunI CAATTG 1 cut(s) 1094
MvaI CCWGG 2 cut(s) 61, 1490
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 854, 1161
NcoI CCATGG 1 cut(s) 1171
NlaIII CATG 8 cut(s) 301, 325, 679, 686, 936, 1109, 1175, 1473
NlaIV GGNNCC 2 cut(s) 57, 1442
NmuCI GTSAC 4 cut(s) 142, 721, 860, 1498
PaeR7I CTCGAG 1 cut(s) 1004
PagI TCATGA 1 cut(s) 1105
PaqCI CACCTGC 1 cut(s) 772
PasI CCCWGGG 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 609
PcsI WCGNNNNNNNCGW 1 cut(s) 879
PdmI GAANNNNTTC 2 cut(s) 213, 423
PfeI GAWTC 5 cut(s) 35, 424, 825, 978, 1334
PflFI GACNNNGTC 1 cut(s) 136
PflMI CCANNNNNTGG 1 cut(s) 1054
PkrI GCNGC 2 cut(s) 844, 847
PleI GAGTC 2 cut(s) 1015, 1212
PpsI GAGTC 2 cut(s) 1015, 1212
PshBI ATTAAT 1 cut(s) 1542
Psp124BI GAGCTC 1 cut(s) 859
Psp6I CCWGG 2 cut(s) 59, 1488
PspEI GGTNACC 1 cut(s) 1498
PspFI CCCAGC 1 cut(s) 838
PspGI CCWGG 2 cut(s) 59, 1488
PspN4I GGNNCC 2 cut(s) 57, 1442
PspPI GGNCC 2 cut(s) 263, 1045
PstI CTGCAG 1 cut(s) 847
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 2 cut(s) 1251, 1440
PsyI GACNNNGTC 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 7 cut(s) 576, 1039, 1100, 1168, 1385, 1450, 1532
RsaNI GTAC 7 cut(s) 575, 1038, 1099, 1167, 1384, 1449, 1531
SacI GAGCTC 1 cut(s) 859
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 6 cut(s) 15, 536, 738, 798, 1256, 1542
SatI GCNGC 2 cut(s) 843, 846
Sau96I GGNCC 2 cut(s) 263, 1045
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 2 cut(s) 1016, 1213
ScrFI CCNGG 2 cut(s) 61, 1490
SduI GDGCHC 1 cut(s) 859
SfaNI GCATC 4 cut(s) 201, 223, 402, 922
SfcI CTRYAG 2 cut(s) 196, 843
Sfr274I CTCGAG 1 cut(s) 1004
SinI GGWCC 2 cut(s) 263, 1045
SlaI CTCGAG 1 cut(s) 1004
SmlI CTYRAG 2 cut(s) 1004, 1255
SmoI CTYRAG 2 cut(s) 1004, 1255
Sse9I AATT 9 cut(s) 12, 48, 127, 538, 564, 740, 1086, 1094, 1539
SsiI CCGC 3 cut(s) 376, 788, 1496
SspI AATATT 1 cut(s) 1286
SspMI CTAG 1 cut(s) 726
SstI GAGCTC 1 cut(s) 859
StyD4I CCNGG 2 cut(s) 59, 1488
StyI CCWWGG 2 cut(s) 587, 1171
TaaI ACNGT 1 cut(s) 1376
TaiI ACGT 1 cut(s) 1532
TaqI TCGA 8 cut(s) 257, 274, 489, 627, 767, 873, 1005, 1146
TaqII GACCGA 1 cut(s) 543
TasI AATT 9 cut(s) 12, 48, 127, 538, 564, 740, 1086, 1094, 1539
TatI WGTACW 2 cut(s) 574, 1098
TfiI GAWTC 5 cut(s) 35, 424, 825, 978, 1334
Tru1I TTAA 6 cut(s) 15, 536, 738, 798, 1256, 1542
Tru9I TTAA 6 cut(s) 15, 536, 738, 798, 1256, 1542
TscAI CASTG 6 cut(s) 367, 856, 1075, 1302, 1394, 1522
TseFI GTSAC 4 cut(s) 142, 721, 860, 1498
TseI GCWGC 2 cut(s) 842, 845
Tsp45I GTSAC 4 cut(s) 142, 721, 860, 1498
TspDTI ATGAA 7 cut(s) 60, 314, 490, 560, 637, 1094, 1326
TspGWI ACGGA 2 cut(s) 94, 1202
TspRI CASTG 6 cut(s) 367, 856, 1075, 1302, 1394, 1522
Tth111I GACNNNGTC 1 cut(s) 136
Van91I CCANNNNNTGG 1 cut(s) 1054
Vha464I CTTAAG 1 cut(s) 1255
VpaK11BI GGWCC 2 cut(s) 263, 1045
VspI ATTAAT 1 cut(s) 1542
XapI RAATTY 4 cut(s) 48, 127, 740, 1086
XhoI CTCGAG 1 cut(s) 1004
XmnI GAANNNNTTC 2 cut(s) 213, 423
XspI CTAG 1 cut(s) 726
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.