Rh5BG129500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000731
Physical Location & Seq
Forward (+)
12569296 .. 12570478
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG129500.1

Sequence Viewer

Length: 612 bp
ATGTCCTTCTCCCGTTCTCCTCTACCCGGAATTATCTGGCCCGTTCCAGCCTCCATTTCCCGTTTCAGAGGACACAGTTCCAAAGGACCCCGTTTGTTCGTTCTTTTTGGCAGAAGAACAAAGGACTTAATGGTAATATTGGAGTTGTGGGATTTGGAATTTAGACATATGCAAAGAGGTAGAACTGCAAGTCTAAAAGGGCAAATCGAAAACCTCAAGGAACATGGAGTAGAAGACACATGGAGAGCATTTGTTGAGACTATGAAAAGGAGAGCTAATACTTCCTCCGATTCTTACTTGAAACAGGAACTCGAGAAAATGGAGAAATGGTTGGACCATGTAGAGCCTTTGGCAAAAGAAATATATCAGGTGCTGGTTGTTCGTCACGGACAGAAAGTCTCTAAGCCTCAAGGAGAACATCATTTTGAAATTCTTCCCAACAGTTTTGGTGCGATTCTGATTAAGCTCTCAAAGCTTCGGTTGAAAGCTATTGCATACTTGGAGCATGGAAACTTGTTGGATGAATTAAACAAGCGAGAACTTGATTATGTAGAGACTTTGATCAAGGAAGTACACAATTTTGTGGTCGTTCAGCAAGTATTGCTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

23.68

Weight (kDa)

9.39

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 158, 429
AfaI GTAC 1 cut(s) 573
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 3 cut(s) 301, 428, 484
AhdI GACNNNNNGTC 1 cut(s) 395
AluBI AGCT 4 cut(s) 275, 466, 475, 488
AluI AGCT 4 cut(s) 275, 466, 475, 488
Alw26I GTCTC 3 cut(s) 251, 403, 548
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 1 cut(s) 38
ApoI RAATTY 2 cut(s) 158, 429
Asp700I GAANNNNTTC 1 cut(s) 432
AspS9I GGNCC 3 cut(s) 39, 86, 334
AsuC2I CCSGG 1 cut(s) 27
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 2 cut(s) 86, 334
BbsI GAAGAC 1 cut(s) 240
BclI TGATCA 1 cut(s) 561
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 3 cut(s) 251, 403, 548
BfaI CTAG 1 cut(s) 605
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 2 cut(s) 86, 334
BmeRI GACNNNNNGTC 1 cut(s) 395
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 3 cut(s) 39, 86, 334
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 27
BpiI GAAGAC 1 cut(s) 240
BpuEI CTTGAG 2 cut(s) 200, 393
BpuMI CCSGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 9
BshFI GGCC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 3 cut(s) 251, 403, 548
BsnI GGCC 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 311
Bsp143I GATC 1 cut(s) 561
BspANI GGCC 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 88
BssMI GATC 1 cut(s) 561
Bst4CI ACNGT 2 cut(s) 77, 443
BstAPI GCANNNNNTGC 1 cut(s) 601
BstDEI CTNAG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 526
BstKTI GATC 1 cut(s) 564
BstMAI GTCTC 3 cut(s) 251, 403, 548
BstMBI GATC 1 cut(s) 561
BstMWI GCNNNNNNNGC 2 cut(s) 472, 601
BstSCI CCNGG 1 cut(s) 25
BstV2I GAAGAC 1 cut(s) 240
BsuRI GGCC 1 cut(s) 40
BtsCI GGATG 1 cut(s) 526
CaiI CAGNNNCTG 1 cut(s) 373
Cfr13I GGNCC 3 cut(s) 39, 86, 334
Csp6I GTAC 1 cut(s) 572
CviAII CATG 4 cut(s) 224, 240, 338, 506
CviJI RGCY 8 cut(s) 40, 50, 275, 346, 406, 466, 475, 488
CviKI_1 RGCY 8 cut(s) 40, 50, 275, 346, 406, 466, 475, 488
CviQI GTAC 1 cut(s) 572
DdeI CTNAG 1 cut(s) 402
DpnI GATC 1 cut(s) 563
DpnII GATC 1 cut(s) 561
DriI GACNNNNNGTC 1 cut(s) 395
Eam1105I GACNNNNNGTC 1 cut(s) 395
Eco47I GGWCC 2 cut(s) 86, 334
Eco88I CYCGRG 1 cut(s) 311
EcoO109I RGGNCCY 1 cut(s) 86
FaeI CATG 4 cut(s) 227, 243, 341, 509
FatI CATG 4 cut(s) 223, 239, 337, 505
FauNDI CATATG 1 cut(s) 168
FbaI TGATCA 1 cut(s) 561
FokI GGATG 1 cut(s) 533
FspBI CTAG 1 cut(s) 605
HaeIII GGCC 1 cut(s) 40
HapII CCGG 1 cut(s) 27
Hin1II CATG 4 cut(s) 227, 243, 341, 509
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 2 cut(s) 290, 454
HpaII CCGG 1 cut(s) 27
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 3 cut(s) 68, 289, 459
Hpy188III TCNNGA 1 cut(s) 313
Hpy8I GTNNAC 1 cut(s) 574
HpyAV CCTTC 1 cut(s) 16
HpyCH4III ACNGT 2 cut(s) 77, 443
HpyCH4V TGCA 3 cut(s) 172, 188, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 472, 601
HpyF3I CTNAG 1 cut(s) 402
Hsp92II CATG 4 cut(s) 227, 243, 341, 509
Ksp22I TGATCA 1 cut(s) 561
Kzo9I GATC 1 cut(s) 561
LmnI GCTCC 1 cut(s) 502
LpnPI CCDG 6 cut(s) 22, 40, 60, 290, 353, 359
MaeI CTAG 1 cut(s) 605
MaeIII GTNAC 1 cut(s) 383
MalI GATC 1 cut(s) 563
MboI GATC 1 cut(s) 561
MboII GAAGA 3 cut(s) 126, 245, 425
MluCI AATT 5 cut(s) 30, 158, 429, 524, 577
MmeI TCCRAC 2 cut(s) 312, 498
MnlI CCTC 7 cut(s) 30, 61, 62, 170, 224, 295, 417
MroXI GAANNNNTTC 1 cut(s) 432
MseI TTAA 3 cut(s) 128, 462, 527
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 472, 601
NciI CCSGG 1 cut(s) 27
NdeI CATATG 1 cut(s) 168
NdeII GATC 1 cut(s) 561
NlaIII CATG 4 cut(s) 227, 243, 341, 509
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 311
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 2 cut(s) 290, 454
PpuMI RGGWCCY 1 cut(s) 86
Psp5II RGGWCCY 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 3 cut(s) 39, 86, 334
PspPPI RGGWCCY 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 373
RsaI GTAC 1 cut(s) 573
RsaNI GTAC 1 cut(s) 572
SaqAI TTAA 3 cut(s) 128, 462, 527
Sau3AI GATC 1 cut(s) 561
Sau96I GGNCC 3 cut(s) 39, 86, 334
ScrFI CCNGG 1 cut(s) 27
SetI ASST 7 cut(s) 181, 216, 277, 372, 468, 477, 490
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 2 cut(s) 86, 334
SlaI CTCGAG 1 cut(s) 311
SmlI CTYRAG 3 cut(s) 215, 311, 408
SmoI CTYRAG 3 cut(s) 215, 311, 408
Sse9I AATT 5 cut(s) 30, 158, 429, 524, 577
SspI AATATT 1 cut(s) 138
SspMI CTAG 1 cut(s) 605
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 2 cut(s) 77, 443
TaqI TCGA 2 cut(s) 207, 312
TasI AATT 5 cut(s) 30, 158, 429, 524, 577
TatI WGTACW 1 cut(s) 571
TfiI GAWTC 2 cut(s) 290, 454
Tru1I TTAA 3 cut(s) 128, 462, 527
Tru9I TTAA 3 cut(s) 128, 462, 527
TseFI GTSAC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 2 cut(s) 278, 537
TspGWI ACGGA 1 cut(s) 402
VpaK11BI GGWCC 2 cut(s) 86, 334
XapI RAATTY 2 cut(s) 158, 429
XhoI CTCGAG 1 cut(s) 311
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.