Rh3CG257600

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000731
Physical Location & Seq
Reverse (-)
25122231 .. 25123370
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG257600.1

Sequence Viewer

Length: 1140 bp
ATGAGAGAATGGGCCACTGCTATTTATGATTCGGACGCAGAGGTTCGTGGAAGCTACGCTGAAGATATCAGCAACATTGATCAGGAGGAACTAGCAGAAATAATGTTGGTCGATGGTTGCTTCTTATTGGAACTTTTCATCAGGTTTTACCTATACGTGCGCGGGAAGTTAGAAGGCCCTGAATCTGATCCAATTCTAGAAAGTGCTTGGATGATTGCAGATCTACGGCACGATCTGGCATTGCTTGAGAACCAAATCCCCTTTTGTATTATACTTGATTTGTATGAGGCTATTAGTGGTCATCAGATACTTGTCGACAATGAGTTGCCTCCACCACATGGTCTTGCTCTTCACTTCTTTCAACCTGTGAGCCTCAAGGAAAGCATAGTCACAGAGTGGATACCCGAGTACAAGCATTTGCTGGATATCTTGCACAAGTTCTATTTCCTCCCAACTAAAACTACCGACTTGTCTATGAGGCTCAATATGGAATTGATCCCTGAGGAGCAGCGTCTTAGGGCAGTCCAAAAGTATAAAGTATGGGGACTCAATTATTGTGCCTCCGAACTTTTGGAGTCTGGAGTCGAGTTTGACATTGGCTCCAGCCAGGACCAGTTGCTGGACATAACTTTCCGTGAGGGAGTAATCAGAATCCCACCACTGTTTATTCACGAGACAACGAGTTCATTGCTGAGGAACCTGATTGCTTATGAGCAATGCAGTCTTAGTAGTACGCATGGCGTTACATCACATGCCTGCCTCATGAAGAGTCTCATTGGTTCTTCACGGGATAGCAATCTGCTTCGGGAGAAGGGGATAATCAAATATAACCATTGGATTGGAGATGAAGAGGAATATTTGACTCAAATATTCAAAGGTATACTAGATGAAGTTGTTGTCAAGGAATTCCATTTTAGAACTTTGTGCGATGAAGTGAATGAGTACTGTACATCCTGGTTTCATCTGAGAAAACTCAAAGTATTTCTGAGGGTGCGGATTCAAAGATATATGAGGCTTCTTTTCAGCACTTATTTTTCTTCTCCTTGGAGTTTCATATCATTCATGGCAGCGCTCACTATTCTCATTCTCACATTTTTGCAGACATACTATTCCATGCACACAGCTCGTACTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

379

Amino Acids

44.19

Weight (kDa)

5.16

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 3 - 363 1.7e-77 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 338, 619
AccI GTMKAC 2 cut(s) 315, 880
AccII CGCG 1 cut(s) 162
AciI CCGC 2 cut(s) 162, 994
AclWI GGATC 2 cut(s) 182, 490
AcsI RAATTY 1 cut(s) 905
AcuI CTGAAG 1 cut(s) 81
AdeI CACNNNGTG 1 cut(s) 396
AfaI GTAC 5 cut(s) 410, 733, 944, 949, 1129
AfeI AGCGCT 1 cut(s) 1071
AfiI CCNNNNNNNGG 2 cut(s) 338, 619
AgsI TTSAA 3 cut(s) 362, 874, 1001
AjnI CCWGG 2 cut(s) 606, 953
AluBI AGCT 2 cut(s) 54, 1124
AluI AGCT 2 cut(s) 54, 1124
Alw26I GTCTC 2 cut(s) 668, 776
AlwI GGATC 2 cut(s) 182, 490
AlwNI CAGNNNCTG 1 cut(s) 619
Ama87I CYCGRG 1 cut(s) 404
Aor51HI AGCGCT 1 cut(s) 1071
AoxI GGCC 2 cut(s) 12, 175
ApeKI GCWGC 2 cut(s) 508, 1067
ApoI RAATTY 1 cut(s) 905
AseI ATTAAT 1 cut(s) 1134
AspLEI GCGC 2 cut(s) 162, 1072
AspS9I GGNCC 3 cut(s) 12, 176, 610
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 1 cut(s) 610
AxyI CCTNAGG 1 cut(s) 501
BaeI ACNNNNGTAYC 2 cut(s) 392, 425
BauI CACGAG 1 cut(s) 671
BbvCI CCTCAGC 1 cut(s) 692
BbvI GCAGC 2 cut(s) 520, 1079
BccI CCATC 1 cut(s) 107
BceAI ACGGC 1 cut(s) 242
BcgI CGANNNNNNTGC 4 cut(s) 670, 704, 1106, 1140
BciT130I CCWGG 2 cut(s) 608, 955
BciVI GTATCC 1 cut(s) 393
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 668, 776
BfaI CTAG 3 cut(s) 92, 197, 884
BfoI RGCGCY 1 cut(s) 1073
BfuI GTATCC 1 cut(s) 393
BglII AGATCT 1 cut(s) 220
BisI GCNGC 2 cut(s) 509, 1068
BlsI GCNGC 2 cut(s) 510, 1069
BmcAI AGTACT 1 cut(s) 944
Bme1390I CCNGG 2 cut(s) 608, 955
Bme18I GGWCC 1 cut(s) 610
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 12, 176, 610
BmiI GGNNCC 2 cut(s) 601, 698
BmrFI CCNGG 2 cut(s) 608, 955
BpmI CTGGAG 2 cut(s) 586, 600
Bpu10I CCTNAGC 1 cut(s) 692
BpuEI CTTGAG 2 cut(s) 266, 359
BsaAI YACGTR 1 cut(s) 157
BsaBI GATNNNNATC 1 cut(s) 795
BsaJI CCNNGG 1 cut(s) 1043
BsaXI ACNNNNNCTCC 2 cut(s) 584, 614
Bsc4I CCNNNNNNNGG 2 cut(s) 338, 619
Bse1I ACTGG 1 cut(s) 613
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 3 cut(s) 239, 686, 722
Bse8I GATNNNNATC 1 cut(s) 795
BseBI CCWGG 2 cut(s) 608, 955
BseDI CCNNGG 1 cut(s) 1043
BseGI GGATG 2 cut(s) 216, 950
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 2 cut(s) 338, 619
BseMI GCAATG 3 cut(s) 239, 686, 722
BseMII CTCAG 4 cut(s) 492, 683, 956, 977
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 2 cut(s) 520, 1079
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 2 cut(s) 14, 177
BsiHKCI CYCGRG 1 cut(s) 404
BslFI GGGAC 1 cut(s) 558
BslI CCNNNNNNNGG 2 cut(s) 338, 619
BsmAI GTCTC 2 cut(s) 668, 776
BsmFI GGGAC 1 cut(s) 558
BsnI GGCC 2 cut(s) 14, 177
BsoBI CYCGRG 1 cut(s) 404
Bsp1407I TGTACA 1 cut(s) 947
Bsp143I GATC 5 cut(s) 79, 187, 220, 232, 495
BspACI CCGC 2 cut(s) 162, 994
BspANI GGCC 2 cut(s) 14, 177
BspCNI CTCAG 4 cut(s) 493, 684, 957, 978
BspFNI CGCG 1 cut(s) 162
BspHI TCATGA 1 cut(s) 762
BspLI GGNNCC 2 cut(s) 601, 698
BspPI GGATC 2 cut(s) 182, 490
BspQI GCTCTTC 1 cut(s) 354
BsrDI GCAATG 3 cut(s) 239, 686, 722
BsrGI TGTACA 1 cut(s) 947
BsrI ACTGG 1 cut(s) 613
BssECI CCNNGG 1 cut(s) 1043
BssMI GATC 5 cut(s) 79, 187, 220, 232, 495
BssNAI GTATAC 1 cut(s) 881
BssSI CACGAG 1 cut(s) 671
BssT1I CCWWGG 1 cut(s) 1043
Bst1107I GTATAC 1 cut(s) 881
Bst2BI CACGAG 1 cut(s) 671
Bst2UI CCWGG 2 cut(s) 608, 955
Bst4CI ACNGT 2 cut(s) 663, 947
Bst6I CTCTTC 3 cut(s) 354, 761, 843
BstAUI TGTACA 1 cut(s) 947
BstBAI YACGTR 1 cut(s) 157
BstC8I GCNNGC 1 cut(s) 757
BstDEI CTNAG 6 cut(s) 501, 515, 692, 725, 965, 986
BstF5I GGATG 2 cut(s) 216, 950
BstFNI CGCG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 1073
BstHHI GCGC 2 cut(s) 162, 1072
BstKTI GATC 5 cut(s) 82, 190, 223, 235, 498
BstMAI GTCTC 2 cut(s) 668, 776
BstMBI GATC 5 cut(s) 79, 187, 220, 232, 495
BstNI CCWGG 2 cut(s) 608, 955
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 2 cut(s) 606, 953
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 2 cut(s) 520, 1079
BstX2I RGATCY 1 cut(s) 220
BstXI CCANNNNNNTGG 1 cut(s) 839
BstYI RGATCY 1 cut(s) 220
BstZ17I GTATAC 1 cut(s) 881
Bsu36I CCTNAGG 1 cut(s) 501
BsuI GTATCC 1 cut(s) 393
BsuRI GGCC 2 cut(s) 14, 177
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 2 cut(s) 216, 950
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 15, 659
Cac8I GCNNGC 1 cut(s) 757
CaiI CAGNNNCTG 1 cut(s) 619
CciI TCATGA 1 cut(s) 762
CfoI GCGC 2 cut(s) 162, 1072
Cfr13I GGNCC 3 cut(s) 12, 176, 610
CseI GACGC 2 cut(s) 44, 500
Csp6I GTAC 5 cut(s) 409, 732, 943, 948, 1128
CviAII CATG 6 cut(s) 338, 737, 752, 763, 1063, 1114
CviQI GTAC 5 cut(s) 409, 732, 943, 948, 1128
DdeI CTNAG 6 cut(s) 501, 515, 692, 725, 965, 986
DpnI GATC 5 cut(s) 81, 189, 222, 234, 497
DpnII GATC 5 cut(s) 79, 187, 220, 232, 495
DraIII CACNNNGTG 1 cut(s) 396
Eam1104I CTCTTC 3 cut(s) 354, 761, 843
EarI CTCTTC 3 cut(s) 354, 761, 843
Eco130I CCWWGG 1 cut(s) 1043
Eco32I GATATC 2 cut(s) 67, 427
Eco47I GGWCC 1 cut(s) 610
Eco47III AGCGCT 1 cut(s) 1071
Eco57I CTGAAG 1 cut(s) 81
Eco81I CCTNAGG 1 cut(s) 501
Eco88I CYCGRG 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 2 cut(s) 606, 953
EcoRV GATATC 2 cut(s) 67, 427
EcoT14I CCWWGG 1 cut(s) 1043
ErhI CCWWGG 1 cut(s) 1043
FaeI CATG 6 cut(s) 341, 740, 755, 766, 1066, 1117
FaqI GGGAC 1 cut(s) 558
FatI CATG 6 cut(s) 337, 736, 751, 762, 1062, 1113
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 2 cut(s) 315, 880
Fnu4HI GCNGC 2 cut(s) 509, 1068
FokI GGATG 2 cut(s) 223, 937
Fsp4HI GCNGC 2 cut(s) 509, 1068
FspBI CTAG 3 cut(s) 92, 197, 884
GlaI GCGC 2 cut(s) 161, 1071
GluI GCNGC 2 cut(s) 509, 1068
GsuI CTGGAG 2 cut(s) 586, 600
HaeII RGCGCY 1 cut(s) 1073
HaeIII GGCC 2 cut(s) 14, 177
HgaI GACGC 2 cut(s) 44, 500
HhaI GCGC 2 cut(s) 162, 1072
Hin1II CATG 6 cut(s) 341, 740, 755, 766, 1066, 1117
Hin6I GCGC 2 cut(s) 160, 1070
HinP1I GCGC 2 cut(s) 160, 1070
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 9 cut(s) 29, 182, 546, 575, 582, 651, 769, 862, 997
Hpy166II GTNNAC 2 cut(s) 316, 881
Hpy188I TCNGA 7 cut(s) 34, 187, 306, 565, 650, 966, 987
Hpy188III TCNNGA 6 cut(s) 83, 197, 579, 671, 763, 806
Hpy8I GTNNAC 2 cut(s) 316, 881
HpyAV CCTTC 2 cut(s) 167, 805
HpyCH4III ACNGT 2 cut(s) 663, 947
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 5 cut(s) 218, 433, 720, 1099, 1117
HpyF3I CTNAG 6 cut(s) 501, 515, 692, 725, 965, 986
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 6 cut(s) 341, 740, 755, 766, 1066, 1117
HspAI GCGC 2 cut(s) 160, 1070
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 5 cut(s) 79, 187, 220, 232, 495
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 2 cut(s) 505, 605
Lsp1109I GCAGC 2 cut(s) 520, 1079
MaeI CTAG 3 cut(s) 92, 197, 884
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 388, 742
MalI GATC 5 cut(s) 81, 189, 222, 234, 497
MboI GATC 5 cut(s) 79, 187, 220, 232, 495
MboII GAAGA 6 cut(s) 74, 341, 774, 778, 860, 1029
MflI RGATCY 1 cut(s) 220
MluCI AATT 5 cut(s) 192, 491, 550, 905, 1135
MlyI GAGTC 5 cut(s) 540, 584, 591, 778, 856
MseI TTAA 2 cut(s) 1134, 1138
MspR9I CCNGG 2 cut(s) 608, 955
MvaI CCWGG 2 cut(s) 608, 955
MvnI CGCG 1 cut(s) 162
NdeII GATC 5 cut(s) 79, 187, 220, 232, 495
NlaIII CATG 6 cut(s) 341, 740, 755, 766, 1066, 1117
NlaIV GGNNCC 2 cut(s) 601, 698
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 755
PacI TTAATTAA 1 cut(s) 1138
PagI TCATGA 1 cut(s) 762
PciSI GCTCTTC 1 cut(s) 354
PfeI GAWTC 4 cut(s) 29, 182, 651, 997
PflMI CCANNNNNTGG 2 cut(s) 338, 619
PkrI GCNGC 2 cut(s) 510, 1069
PleI GAGTC 5 cut(s) 540, 583, 590, 777, 856
PpsI GAGTC 5 cut(s) 540, 583, 590, 777, 856
Ppu21I YACGTR 1 cut(s) 157
PshBI ATTAAT 1 cut(s) 1134
Psp6I CCWGG 2 cut(s) 606, 953
PspGI CCWGG 2 cut(s) 606, 953
PspN4I GGNNCC 2 cut(s) 601, 698
PspPI GGNCC 3 cut(s) 12, 176, 610
PstNI CAGNNNCTG 1 cut(s) 619
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 5 cut(s) 410, 733, 944, 949, 1129
RsaNI GTAC 5 cut(s) 409, 732, 943, 948, 1128
SalI GTCGAC 1 cut(s) 314
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 2 cut(s) 1134, 1138
SatI GCNGC 2 cut(s) 509, 1068
Sau3AI GATC 5 cut(s) 79, 187, 220, 232, 495
Sau96I GGNCC 3 cut(s) 12, 176, 610
ScaI AGTACT 1 cut(s) 944
SchI GAGTC 5 cut(s) 540, 584, 591, 778, 856
ScrFI CCNGG 2 cut(s) 608, 955
SetI ASST 9 cut(s) 45, 56, 146, 153, 159, 367, 702, 880, 1126
SinI GGWCC 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 245, 374
SmoI CTYRAG 2 cut(s) 245, 374
Sse9I AATT 5 cut(s) 192, 491, 550, 905, 1135
SsiI CCGC 2 cut(s) 162, 994
SspI AATATT 2 cut(s) 857, 870
SspMI CTAG 3 cut(s) 92, 197, 884
StyD4I CCNGG 2 cut(s) 606, 953
StyI CCWWGG 1 cut(s) 1043
TaaI ACNGT 2 cut(s) 663, 947
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 111, 315, 585
TasI AATT 5 cut(s) 192, 491, 550, 905, 1135
TatI WGTACW 3 cut(s) 408, 942, 947
TfiI GAWTC 4 cut(s) 29, 182, 651, 997
Tru1I TTAA 2 cut(s) 1134, 1138
Tru9I TTAA 2 cut(s) 1134, 1138
TscAI CASTG 2 cut(s) 22, 666
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 2 cut(s) 508, 1067
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 9 cut(s) 127, 675, 779, 861, 903, 945, 950, 1042, 1051
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 22, 666
Van91I CCANNNNNTGG 2 cut(s) 338, 619
VpaK11BI GGWCC 1 cut(s) 610
VspI ATTAAT 1 cut(s) 1134
XapI RAATTY 1 cut(s) 905
XbaI TCTAGA 1 cut(s) 196
XceI RCATGY 1 cut(s) 755
XmiI GTMKAC 2 cut(s) 315, 880
XspI CTAG 3 cut(s) 92, 197, 884
ZrmI AGTACT 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.