Prupe.6G274100_v2.0.a1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000731
Physical Location & Seq
Forward (+)
25901741 .. 25903697
1957 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G274100.1

Sequence Viewer

Length: 1530 bp
ATGATGAATCAATCAGAAGAGGAAACACATCAGCTGGAAGGCGAGGAAGACGAAGAAGAGACATTGAAGACTAAGTGGGTATTGGAGATTAAAGAGAGACTCGGAACAGTTAAGGAAGCAGAAGAATCATGCATCTATCAAGTCCCAAGCAAACTTCGAAAGGTGAGAGAGGATGCCTACAATCCTCGCGTCGTCTCAATTGGACCTTTTCATCGAAAAAACACTGACCTTGTAGCTGCTATGAGAGAGCACAAATGGCGTTACATGTTAAGCTTTCTGCAGCAAACGGATGATCCTGACGAGTCTCATGAATGCTTGGAACATTGCATGGATGCTATTTATAATTTAGACAAGGAGGTTCGTCAATGCTACGCCGAAAACATCGACTATGAGAAGAATGAGCTAGCAAGAATAATGTTACTTGATGGCTGCTTCATCTTAGAACTCTTCCTCAGGTACTACGCCAAGAAAGTGGAAAAAGAAGACGGCCCTGATCCAGTACTAAAAAGTGCTTGGATGATCGCGGCTCTTCAGCATGATTTGGCTTTGCTTGAAAACCAAATCCCTTTCTTTATTCTCAAACGTTTGTATGACATTGTCCAGCCTCGTGTCGTGATCAACGGCCATGAAGCACCACACTCGGTTGCTGGCCTTGCTCTCATGTTCTTTCAACCTTTGAGCCGAAAGCCGATAGCTAAAGATCAACATGAGCTGGGGACAGACTTCAAGCACTTGCTTGATCTCTTGCACAAATTCTACTTCCTCAGAGCTGGAGTGCCTGGCCATGAGGTGTCAATTAAGGTCAAGGTCAATCATGTGGAATTGGTTTCCAAGGTTAAATCACAACAATCTGCATGCCTTTTTTTCAGAAAAATATTTAAGAGAAGAAAGCAACAAACTGCTTCATGCCTTCCTGAACCTGATGAGCCAGCAACAAACAACAAGTGGGGATTCAACTATTGCGCTTCGGAACTTTTAGAGTCTGGGATCCAGTTTCAGATTGGTTCTTGGGAGGACAATTTATTGAACATAACATTCAGCAATGGAGTCATCAGCGTTCCCCAGTTGATCATTGACGAGGCTACCAGTTCAGTGTTCAGGAACCTCCTTGCCTTCGAGCAATACAGCCTTAGCTCTACACATGGCGTGACGTCGTATGCCTTCCTCATGAAAAGTCTCATCCGTTCCTCAGCGGACACCAAGCTGCTCCGAGAAAAAGGGATCATAAGGCATAATAGGATTGGAGACCATGAGTATTTGAGTCAGTTCAAGGATATTCTTGATGAAGTTGTTGTCAAGGACGACTTCTATTTTGATACATTGCGAGACCAAGTGAACGAGTATTGCACGTCCTGGTGCAGCTTGAGCAAGCTTCAAGTATTTCTGAGGGTGCGATTTCTAAGAGAAATAAGGGTCCTTTTCAGCACCTACTTCTCTTCCACATGGAGTTTCACATCTTTCCTGGCTGCGTTTGCCCTTCTCATTCTCACATCCATGCAAACATACTACACCATTCACCCTCGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

510

Amino Acids

59.32

Weight (kDa)

6.05

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 342
AatII GACGTC 1 cut(s) 1154
AccII CGCG 2 cut(s) 189, 524
AciI CCGC 2 cut(s) 524, 1193
AclI AACGTT 1 cut(s) 583
AclWI GGATC 5 cut(s) 287, 488, 982, 995, 1229
AcoI YGGCCR 2 cut(s) 622, 781
AcsI RAATTY 1 cut(s) 752
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 1 cut(s) 1151
AfaI GTAC 2 cut(s) 458, 501
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 8 cut(s) 67, 554, 671, 727, 955, 1027, 1270, 1376
AjiI CACGTC 1 cut(s) 1350
AjnI CCWGG 3 cut(s) 778, 1352, 1461
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 7 cut(s) 53, 91, 199, 309, 1181, 1239, 1320
AlwI GGATC 5 cut(s) 287, 488, 982, 995, 1229
AoxI GGCC 4 cut(s) 487, 622, 649, 781
ApeKI GCWGC 6 cut(s) 236, 280, 429, 1204, 1359, 1466
ApoI RAATTY 1 cut(s) 752
ArsI GACNNNNNNTTYG 2 cut(s) 1295, 1327
AspLEI GCGC 1 cut(s) 965
AspS9I GGNCC 3 cut(s) 203, 488, 1414
AsuHPI GGTGA 2 cut(s) 175, 1508
AsuII TTCGAA 1 cut(s) 157
AsuNHI GCTAGC 1 cut(s) 403
AvaII GGWCC 2 cut(s) 203, 1414
AxyI CCTNAGG 1 cut(s) 452
BalI TGGCCA 1 cut(s) 783
BamHI GGATCC 1 cut(s) 987
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 3 cut(s) 54, 74, 489
Bbv12I GWGCWC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 1189
BbvI GCAGC 6 cut(s) 223, 292, 416, 1191, 1371, 1453
BccI CCATC 1 cut(s) 419
BceAI ACGGC 2 cut(s) 502, 637
BcgI CGANNNNNNTGC 2 cut(s) 621, 655
BciT130I CCWGG 3 cut(s) 780, 1354, 1463
BclI TGATCA 2 cut(s) 615, 1068
BcoDI GTCTC 7 cut(s) 53, 91, 199, 309, 1181, 1239, 1320
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 278
BisI GCNGC 7 cut(s) 237, 281, 430, 525, 1205, 1360, 1467
BlsI GCNGC 7 cut(s) 238, 282, 431, 526, 1206, 1361, 1468
BmcAI AGTACT 1 cut(s) 501
Bme1390I CCNGG 3 cut(s) 780, 1354, 1463
Bme18I GGWCC 2 cut(s) 203, 1414
BmgBI CACGTC 1 cut(s) 1350
BmgT120I GGNCC 3 cut(s) 203, 488, 1414
BmiI GGNNCC 3 cut(s) 989, 1103, 1415
BmrFI CCNGG 3 cut(s) 780, 1354, 1463
BmrI ACTGGG 1 cut(s) 1057
BmsI GCATC 3 cut(s) 141, 163, 322
BmtI GCTAGC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 1057
BpiI GAAGAC 3 cut(s) 54, 74, 489
BpmI CTGGAG 1 cut(s) 792
Bpu10I CCTNAGC 2 cut(s) 1130, 1189
Bpu14I TTCGAA 1 cut(s) 157
BpuEI CTTGAG 1 cut(s) 1384
BsaHI GRCGYC 1 cut(s) 1151
BsaI GGTCTC 2 cut(s) 1239, 1320
BsaJI CCNNGG 1 cut(s) 831
Bse1I ACTGG 4 cut(s) 497, 991, 1063, 1086
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 3 cut(s) 322, 1048, 1319
BseBI CCWGG 3 cut(s) 780, 1354, 1463
BseDI CCNNGG 1 cut(s) 831
BseGI GGATG 6 cut(s) 178, 295, 337, 522, 1179, 1490
BseMI GCAATG 3 cut(s) 322, 1048, 1319
BseMII CTCAG 4 cut(s) 466, 778, 1203, 1376
BseNI ACTGG 4 cut(s) 497, 991, 1063, 1086
BseXI GCAGC 6 cut(s) 223, 292, 416, 1191, 1371, 1453
BseYI CCCAGC 1 cut(s) 712
BsgI GTGCAG 1 cut(s) 1378
Bsh1236I CGCG 2 cut(s) 189, 524
BshFI GGCC 4 cut(s) 489, 624, 651, 783
BsiHKAI GWGCWC 1 cut(s) 252
BslFI GGGAC 2 cut(s) 128, 730
BsmAI GTCTC 7 cut(s) 53, 91, 199, 309, 1181, 1239, 1320
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 2 cut(s) 128, 730
BsmI GAATGC 1 cut(s) 317
BsnI GGCC 4 cut(s) 489, 624, 651, 783
Bso31I GGTCTC 2 cut(s) 1239, 1320
Bsp119I TTCGAA 1 cut(s) 157
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
BspACI CCGC 2 cut(s) 524, 1193
BspANI GGCC 4 cut(s) 489, 624, 651, 783
BspCNI CTCAG 4 cut(s) 465, 777, 1202, 1377
BspFNI CGCG 2 cut(s) 189, 524
BspHI TCATGA 2 cut(s) 307, 1167
BspLI GGNNCC 3 cut(s) 989, 1103, 1415
BspMAI CTGCAG 1 cut(s) 282
BspOI GCTAGC 1 cut(s) 407
BspPI GGATC 5 cut(s) 287, 488, 982, 995, 1229
BspQI GCTCTTC 1 cut(s) 534
BspT104I TTCGAA 1 cut(s) 157
BspTNI GGTCTC 2 cut(s) 1239, 1320
BsrDI GCAATG 3 cut(s) 322, 1048, 1319
BsrI ACTGG 4 cut(s) 497, 991, 1063, 1086
BssECI CCNNGG 1 cut(s) 831
BssMI GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
BssNI GRCGYC 1 cut(s) 1151
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 831
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 3 cut(s) 780, 1354, 1463
Bst4CI ACNGT 1 cut(s) 109
Bst6I CTCTTC 5 cut(s) 12, 51, 452, 534, 1441
BstACI GRCGYC 1 cut(s) 1151
BstBI TTCGAA 1 cut(s) 157
BstC8I GCNNGC 5 cut(s) 405, 649, 856, 930, 1370
BstDEI CTNAG 8 cut(s) 72, 439, 452, 764, 1130, 1189, 1385, 1400
BstF5I GGATG 6 cut(s) 178, 295, 337, 522, 1179, 1490
BstFNI CGCG 2 cut(s) 189, 524
BstHHI GCGC 1 cut(s) 965
BstKTI GATC 9 cut(s) 295, 496, 522, 618, 703, 742, 990, 1071, 1224
BstMAI GTCTC 7 cut(s) 53, 91, 199, 309, 1181, 1239, 1320
BstMBI GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
BstMWI GCNNNNNNNGC 4 cut(s) 256, 653, 1365, 1472
BstNI CCWGG 3 cut(s) 780, 1354, 1463
BstNSI RCATGY 2 cut(s) 268, 858
BstSCI CCNGG 3 cut(s) 778, 1352, 1461
BstSFI CTRYAG 1 cut(s) 278
BstUI CGCG 2 cut(s) 189, 524
BstV1I GCAGC 6 cut(s) 223, 292, 416, 1191, 1371, 1453
BstV2I GAAGAC 3 cut(s) 54, 74, 489
BstX2I RGATCY 1 cut(s) 987
BstXI CCANNNNNNTGG 1 cut(s) 472
BstYI RGATCY 1 cut(s) 987
Bsu36I CCTNAGG 1 cut(s) 452
BsuRI GGCC 4 cut(s) 489, 624, 651, 783
BtrI CACGTC 1 cut(s) 1350
BtsCI GGATG 6 cut(s) 178, 295, 337, 522, 1179, 1490
BtsIMutI CAGTG 2 cut(s) 222, 1098
Cac8I GCNNGC 5 cut(s) 405, 649, 856, 930, 1370
CciI TCATGA 2 cut(s) 307, 1167
CfoI GCGC 1 cut(s) 965
Cfr13I GGNCC 3 cut(s) 203, 488, 1414
CseI GACGC 1 cut(s) 178
Csp6I GTAC 2 cut(s) 457, 500
CviQI GTAC 2 cut(s) 457, 500
DdeI CTNAG 8 cut(s) 72, 439, 452, 764, 1130, 1189, 1385, 1400
DpnI GATC 9 cut(s) 294, 495, 521, 617, 702, 741, 989, 1070, 1223
DpnII GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
EaeI YGGCCR 2 cut(s) 622, 781
Eam1104I CTCTTC 5 cut(s) 12, 51, 452, 534, 1441
EarI CTCTTC 5 cut(s) 12, 51, 452, 534, 1441
Eco130I CCWWGG 1 cut(s) 831
Eco31I GGTCTC 2 cut(s) 1239, 1320
Eco47I GGWCC 2 cut(s) 203, 1414
Eco57I CTGAAG 1 cut(s) 515
Eco81I CCTNAGG 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 1414
EcoRII CCWGG 3 cut(s) 778, 1352, 1461
EcoT14I CCWWGG 1 cut(s) 831
EcoT22I ATGCAT 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 831
Esp3I CGTCTC 1 cut(s) 199
FalI AAGNNNNNCTT 2 cut(s) 1289, 1321
FaqI GGGAC 2 cut(s) 128, 730
FbaI TGATCA 2 cut(s) 615, 1068
Fnu4HI GCNGC 7 cut(s) 237, 281, 430, 525, 1205, 1360, 1467
FokI GGATG 6 cut(s) 185, 302, 344, 529, 1166, 1477
Fsp4HI GCNGC 7 cut(s) 237, 281, 430, 525, 1205, 1360, 1467
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 964
GluI GCNGC 7 cut(s) 237, 281, 430, 525, 1205, 1360, 1467
GsaI CCCAGC 1 cut(s) 716
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 4 cut(s) 489, 624, 651, 783
HgaI GACGC 1 cut(s) 178
HhaI GCGC 1 cut(s) 965
Hin1I GRCGYC 1 cut(s) 1151
Hin6I GCGC 1 cut(s) 963
HinP1I GCGC 1 cut(s) 963
HindIII AAGCTT 2 cut(s) 271, 1370
HinfI GANTC 8 cut(s) 7, 99, 125, 302, 951, 980, 1047, 1261
HphI GGTGA 2 cut(s) 175, 1508
Hpy166II GTNNAC 1 cut(s) 1336
Hpy188I TCNGA 8 cut(s) 16, 104, 767, 869, 970, 999, 1211, 1386
Hpy188III TCNNGA 7 cut(s) 296, 308, 613, 914, 1099, 1168, 1280
Hpy8I GTNNAC 1 cut(s) 1336
Hpy99I CGWCG 2 cut(s) 194, 1156
HpyAV CCTTC 5 cut(s) 32, 920, 1123, 1171, 1487
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4IV ACGT 3 cut(s) 583, 1151, 1349
HpyCH4V TGCA 8 cut(s) 132, 280, 327, 748, 854, 1347, 1359, 1498
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 653, 1365, 1472
HpyF3I CTNAG 8 cut(s) 72, 439, 452, 764, 1130, 1189, 1385, 1400
HpySE526I ACGT 3 cut(s) 583, 1151, 1349
Hsp92I GRCGYC 1 cut(s) 1151
HspAI GCGC 1 cut(s) 963
Ksp22I TGATCA 2 cut(s) 615, 1068
Kzo9I GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 1 cut(s) 1212
Lsp1109I GCAGC 6 cut(s) 223, 292, 416, 1191, 1371, 1453
LweI GCATC 3 cut(s) 141, 163, 322
MaeI CTAG 1 cut(s) 404
MaeII ACGT 3 cut(s) 583, 1151, 1349
MaeIII GTNAC 3 cut(s) 260, 417, 1147
MalI GATC 9 cut(s) 294, 495, 521, 617, 702, 741, 989, 1070, 1223
MboI GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
MfeI CAATTG 1 cut(s) 198
MflI RGATCY 1 cut(s) 987
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 783
MluCI AATT 6 cut(s) 198, 343, 752, 795, 821, 1018
MluNI TGGCCA 1 cut(s) 783
MlyI GAGTC 5 cut(s) 93, 311, 989, 1056, 1270
Mox20I TGGCCA 1 cut(s) 783
Mph1103I ATGCAT 1 cut(s) 134
MscI TGGCCA 1 cut(s) 783
MseI TTAA 6 cut(s) 90, 111, 269, 798, 837, 879
MslI CAYNNNNRTG 1 cut(s) 1493
Msp20I TGGCCA 1 cut(s) 783
MspA1I CMGCKG 2 cut(s) 34, 1193
MspR9I CCNGG 3 cut(s) 780, 1354, 1463
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 317
MvaI CCWGG 3 cut(s) 780, 1354, 1463
MvnI CGCG 2 cut(s) 189, 524
MwoI GCNNNNNNNGC 4 cut(s) 256, 653, 1365, 1472
NdeII GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
NheI GCTAGC 1 cut(s) 403
NlaIV GGNNCC 3 cut(s) 989, 1103, 1415
NmuCI GTSAC 1 cut(s) 1147
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 2 cut(s) 268, 858
NspV TTCGAA 1 cut(s) 157
PaeI GCATGC 1 cut(s) 858
PagI TCATGA 2 cut(s) 307, 1167
PciI ACATGT 1 cut(s) 264
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 1 cut(s) 317
PfeI GAWTC 3 cut(s) 7, 125, 951
PflFI GACNNNGTC 1 cut(s) 596
PkrI GCNGC 7 cut(s) 238, 282, 431, 526, 1206, 1361, 1468
PleI GAGTC 5 cut(s) 93, 310, 988, 1055, 1269
PpsI GAGTC 5 cut(s) 93, 310, 988, 1055, 1269
PpuMI RGGWCCY 1 cut(s) 1414
PscI ACATGT 1 cut(s) 264
PsiI TTATAA 1 cut(s) 342
Psp1406I AACGTT 1 cut(s) 583
Psp5II RGGWCCY 1 cut(s) 1414
Psp6I CCWGG 3 cut(s) 778, 1352, 1461
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 3 cut(s) 778, 1352, 1461
PspN4I GGNNCC 3 cut(s) 989, 1103, 1415
PspPI GGNCC 3 cut(s) 203, 488, 1414
PspPPI RGGWCCY 1 cut(s) 1414
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 987
PsyI GACNNNGTC 1 cut(s) 596
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 2 cut(s) 458, 501
RsaNI GTAC 2 cut(s) 457, 500
RseI CAYNNNNRTG 1 cut(s) 1493
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 6 cut(s) 90, 111, 269, 798, 837, 879
SatI GCNGC 7 cut(s) 237, 281, 430, 525, 1205, 1360, 1467
Sau3AI GATC 9 cut(s) 292, 493, 519, 615, 700, 739, 987, 1068, 1221
Sau96I GGNCC 3 cut(s) 203, 488, 1414
ScaI AGTACT 1 cut(s) 501
SchI GAGTC 5 cut(s) 93, 311, 989, 1056, 1270
ScrFI CCNGG 3 cut(s) 780, 1354, 1463
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 3 cut(s) 141, 163, 322
SfcI CTRYAG 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 157
SinI GGWCC 2 cut(s) 203, 1414
SmiMI CAYNNNNRTG 1 cut(s) 1493
SmlI CTYRAG 1 cut(s) 1363
SmoI CTYRAG 1 cut(s) 1363
SphI GCATGC 1 cut(s) 858
Sse9I AATT 6 cut(s) 198, 343, 752, 795, 821, 1018
SsiI CCGC 2 cut(s) 524, 1193
SspI AATATT 1 cut(s) 876
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 778, 1352, 1461
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 1 cut(s) 109
TaiI ACGT 3 cut(s) 586, 1154, 1352
TaqI TCGA 4 cut(s) 157, 214, 384, 1116
TasI AATT 6 cut(s) 198, 343, 752, 795, 821, 1018
TatI WGTACW 1 cut(s) 499
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 3 cut(s) 7, 125, 951
Tru1I TTAA 6 cut(s) 90, 111, 269, 798, 837, 879
Tru9I TTAA 6 cut(s) 90, 111, 269, 798, 837, 879
TscAI CASTG 2 cut(s) 229, 1098
TseFI GTSAC 1 cut(s) 1147
TseI GCWGC 6 cut(s) 236, 280, 429, 1204, 1359, 1466
Tsp45I GTSAC 1 cut(s) 1147
TspDTI ATGAA 8 cut(s) 20, 200, 324, 424, 642, 894, 1184, 1299
TspGWI ACGGA 2 cut(s) 302, 1172
TspRI CASTG 2 cut(s) 229, 1098
Tth111I GACNNNGTC 1 cut(s) 596
VpaK11BI GGWCC 2 cut(s) 203, 1414
XapI RAATTY 1 cut(s) 752
XceI RCATGY 2 cut(s) 268, 858
XcmI CCANNNNNNNNNTGG 1 cut(s) 998
XspI CTAG 1 cut(s) 404
ZraI GACGTC 1 cut(s) 1152
ZrmI AGTACT 1 cut(s) 501
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.