RLG00000003409

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000731
Physical Location & Seq
Reverse (-)
48146878 .. 48150994
4117 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003409

Sequence Viewer

Length: 1554 bp
ATGGTGTCTGAAATTAGTGAGTCCAAGGAGAACACACAGGTAACCCAGGAAAAGCTGGATGCTACGTGGAAAGCTAATTTGAAGGAGGTGATTAGTGGTAAACTGGCAACAGTGAATGATAAACAGTCCTCGATCATCACCTTACGCAATAGAAGAATAACAGCGGAGTTGTGCATCTACAGAGTTCCGGAGAAACTGCGAAAAGTGAGGGAGGATGCCTACAAACCCCTTGTTGTCTCAATTGGACCTTTCCATAGAGACAAAGTTAGCCTTGCACCAATGTTACAACACAAATTATGTTACATGGTGTCCTTTCTTAATCAGCGTCCTGAAGTTGTAAATGATGCTGAAAAATGCCTGGATATATGCATCGATAGTATTCATGGTTTTGAAAATATGATTCGTCAATGCTACATTGAAGAGATCAATTGCACAGATGATGTGCTTGCAGAAATGATGTTAGTTGATGGCTGCTTCATACTGGAACTTTTCCTCAGGTTTCACAAAACCCTAAATTACATGAAGCTGGAAGAGAAAAAATCTGATCCGGTATTAAAAAGTGCTTGGAATATGACAGCTCTCAAGCATGATCTAGCATTGCTTGAAAACCAGATCCCCTTCTTTATTCTTGAGATTCTGTATGATGCTATCAGGCCTCGTATCATGAAAAAGTGTAAAGCACCAGAGTCGGTCAGTAGTCTTGCTCTAAGTTTCTTCAAGCCTATGAGCAAAAAGGTGAAGAAGGAAGAGCCCATGAGTAAAAAAGAGCCTATGGGCACAGAATGCAAGCATTTACTCGATCTCTTGCACAAATTCTACTTCCTCCCAGCTCCCTCCATGCCGGACTTCTCAAAGGATGTTAATGATTTGGAATCAAATAATGATCCGGAAAATAAAAATTGTTCCACTGCTCATCCAGAATCCTGTCTGGAAAAGAAAGATTCCCGCCCTGCTGAAGAAAAATGGGGATTCAACTATTGCGCTTCGAAACTTTTGGAGTCTGGAATCGAGTTCCGAGTGTCGTCAAGTACTGAGGAGGAAAGTTTGTTGAACATAACTTTCAATCCGAAGAGTGGGAAAATCCATATACCCAAGCTGGTCATTCATGACGCAACAAGTTTTTCACTCTTCAGGAACTTGATTGCTTTTGAGCAATGCAGTCTTAACAGTACGAACAGCGTCACATCATATGCCTTTCTCATGAGGAGTCTCATCAGTTCCTCACTGGACAGCAAATTGCTTGAGAAAAGAGGGATCATTGAGCACAGTTCTAATGGAGACGAAGAGTATTTGGCTAATAAATTCAAAAGTATACTAGATGGAGTTGATTTGATGGACGACTTTCGTTTTCGGGAGAAGTGCGACGAAGTGAATGAATACCACAGGTCCTGGTACAATCTGAAAAGACTTAAAGTATTTTTTCAAAAACAGAGAAGGATCCTGTGGGCCAATTATCTCTCTAGTGCATGGAAAGTTATGTCAATCCTGGCCGCCATGATCATTCTCATTTTCACTTCCTTACAGACATACTATACAATTCACCCTCGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

518

Amino Acids

59.82

Weight (kDa)

7.84

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 59 - 504 6.8e-101 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1312
AccIII TCCGGA 2 cut(s) 187, 886
AciI CCGC 3 cut(s) 164, 946, 1491
AclWI GGATC 6 cut(s) 539, 607, 878, 1262, 1432, 1445
AcoI YGGCCR 1 cut(s) 1488
AcsI RAATTY 2 cut(s) 812, 1301
AcuI CTGAAG 3 cut(s) 351, 975, 1114
AfaI GTAC 3 cut(s) 1030, 1171, 1394
AfiI CCNNNNNNNGG 1 cut(s) 1073
AjnI CCWGG 4 cut(s) 45, 357, 1388, 1485
AluBI AGCT 6 cut(s) 55, 74, 526, 578, 830, 1096
AluI AGCT 6 cut(s) 55, 74, 526, 578, 830, 1096
Alw21I GWGCWC 1 cut(s) 1266
Alw26I GTCTC 4 cut(s) 241, 252, 1214, 1272
AlwI GGATC 6 cut(s) 539, 607, 878, 1262, 1432, 1445
AlwNI CAGNNNCTG 1 cut(s) 1389
Aor13HI TCCGGA 2 cut(s) 187, 886
AoxI GGCC 3 cut(s) 653, 1446, 1488
ApeKI GCWGC 1 cut(s) 471
ApoI RAATTY 2 cut(s) 812, 1301
AspLEI GCGC 1 cut(s) 983
AspS9I GGNCC 3 cut(s) 245, 1386, 1446
AsuHPI GGTGA 4 cut(s) 100, 130, 748, 1532
AsuII TTCGAA 1 cut(s) 986
AvaII GGWCC 2 cut(s) 245, 1386
AxyI CCTNAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 779
BamHI GGATCC 1 cut(s) 1437
BanII GRGCYC 1 cut(s) 753
Bbv12I GWGCWC 1 cut(s) 1266
BbvI GCAGC 1 cut(s) 458
BccI CCATC 3 cut(s) 461, 1313, 1327
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 4 cut(s) 47, 359, 1390, 1487
BclI TGATCA 1 cut(s) 1497
BcoDI GTCTC 4 cut(s) 241, 252, 1214, 1272
BfaI CTAG 3 cut(s) 593, 1316, 1461
BfmI CTRYAG 1 cut(s) 178
BisI GCNGC 2 cut(s) 472, 1491
BlsI GCNGC 2 cut(s) 473, 1492
BmcAI AGTACT 1 cut(s) 1030
Bme1390I CCNGG 4 cut(s) 47, 359, 1390, 1487
Bme18I GGWCC 2 cut(s) 245, 1386
BmgT120I GGNCC 3 cut(s) 245, 1386, 1446
BmiI GGNNCC 1 cut(s) 1439
BmrFI CCNGG 4 cut(s) 47, 359, 1390, 1487
BmsI GCATC 6 cut(s) 49, 183, 205, 334, 378, 634
Bpu14I TTCGAA 1 cut(s) 986
BpuEI CTTGAG 3 cut(s) 566, 650, 1262
Bsa29I ATCGAT 1 cut(s) 372
BsaAI YACGTR 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 24, 45
BsaWI WCCGGW 3 cut(s) 187, 547, 886
Bsc4I CCNNNNNNNGG 1 cut(s) 1073
Bse1I ACTGG 3 cut(s) 108, 486, 1230
Bse21I CCTNAGG 1 cut(s) 494
Bse3DI GCAATG 2 cut(s) 596, 1160
BseAI TCCGGA 2 cut(s) 187, 886
BseBI CCWGG 4 cut(s) 47, 359, 1390, 1487
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 2 cut(s) 24, 45
BseGI GGATG 4 cut(s) 64, 220, 862, 913
BseLI CCNNNNNNNGG 1 cut(s) 1073
BseMI GCAATG 2 cut(s) 596, 1160
BseMII CTCAG 2 cut(s) 508, 1023
BseNI ACTGG 3 cut(s) 108, 486, 1230
BseRI GAGGAG 2 cut(s) 1049, 1219
BseSI GKGCMC 1 cut(s) 779
BseXI GCAGC 1 cut(s) 458
BseYI CCCAGC 1 cut(s) 826
BshFI GGCC 3 cut(s) 655, 1448, 1490
BshVI ATCGAT 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 1266
BsiSI CCGG 4 cut(s) 188, 548, 842, 887
BslI CCNNNNNNNGG 1 cut(s) 1073
BsmAI GTCTC 4 cut(s) 241, 252, 1214, 1272
BsmBI CGTCTC 1 cut(s) 1272
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 3 cut(s) 655, 1448, 1490
Bsp119I TTCGAA 1 cut(s) 986
Bsp1286I GDGCHC 3 cut(s) 753, 779, 1266
Bsp13I TCCGGA 2 cut(s) 187, 886
BspACI CCGC 3 cut(s) 164, 946, 1491
BspANI GGCC 3 cut(s) 655, 1448, 1490
BspCNI CTCAG 2 cut(s) 507, 1024
BspDI ATCGAT 1 cut(s) 372
BspEI TCCGGA 2 cut(s) 187, 886
BspHI TCATGA 3 cut(s) 663, 1105, 1200
BspLI GGNNCC 1 cut(s) 1439
BspPI GGATC 6 cut(s) 539, 607, 878, 1262, 1432, 1445
BspQI GCTCTTC 1 cut(s) 741
BspT104I TTCGAA 1 cut(s) 986
BsrDI GCAATG 2 cut(s) 596, 1160
BsrI ACTGG 3 cut(s) 108, 486, 1230
BssECI CCNNGG 2 cut(s) 24, 45
BssNAI GTATAC 1 cut(s) 1313
BssT1I CCWWGG 1 cut(s) 24
Bst1107I GTATAC 1 cut(s) 1313
Bst2UI CCWGG 4 cut(s) 47, 359, 1390, 1487
Bst4CI ACNGT 4 cut(s) 112, 126, 1169, 1268
Bst6I CTCTTC 6 cut(s) 414, 525, 741, 1064, 1133, 1278
BstAPI GCANNNNNTGC 1 cut(s) 783
BstBAI YACGTR 1 cut(s) 66
BstBI TTCGAA 1 cut(s) 986
BstC8I GCNNGC 2 cut(s) 447, 788
BstDEI CTNAG 3 cut(s) 494, 707, 1032
BstEII GGTNACC 1 cut(s) 40
BstF5I GGATG 4 cut(s) 64, 220, 862, 913
BstHHI GCGC 1 cut(s) 983
BstMAI GTCTC 4 cut(s) 241, 252, 1214, 1272
BstMWI GCNNNNNNNGC 1 cut(s) 783
BstNI CCWGG 4 cut(s) 47, 359, 1390, 1487
BstPI GGTNACC 1 cut(s) 40
BstSCI CCNGG 4 cut(s) 45, 357, 1388, 1485
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 779
BstV1I GCAGC 1 cut(s) 458
BstX2I RGATCY 2 cut(s) 612, 1437
BstYI RGATCY 2 cut(s) 612, 1437
BstZ17I GTATAC 1 cut(s) 1313
Bsu15I ATCGAT 1 cut(s) 372
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 3 cut(s) 655, 1448, 1490
BsuTUI ATCGAT 1 cut(s) 372
BtsCI GGATG 4 cut(s) 64, 220, 862, 913
BtsI GCAGTG 1 cut(s) 906
BtsIMutI CAGTG 3 cut(s) 117, 906, 1223
Cac8I GCNNGC 2 cut(s) 447, 788
CaiI CAGNNNCTG 1 cut(s) 1389
CciI TCATGA 3 cut(s) 663, 1105, 1200
CfoI GCGC 1 cut(s) 983
Cfr13I GGNCC 3 cut(s) 245, 1386, 1446
ClaI ATCGAT 1 cut(s) 372
CseI GACGC 3 cut(s) 314, 1118, 1168
Csp6I GTAC 3 cut(s) 1029, 1170, 1393
CviQI GTAC 3 cut(s) 1029, 1170, 1393
DdeI CTNAG 3 cut(s) 494, 707, 1032
EaeI YGGCCR 1 cut(s) 1488
Eam1104I CTCTTC 6 cut(s) 414, 525, 741, 1064, 1133, 1278
EarI CTCTTC 6 cut(s) 414, 525, 741, 1064, 1133, 1278
Eco130I CCWWGG 1 cut(s) 24
Eco147I AGGCCT 1 cut(s) 655
Eco24I GRGCYC 1 cut(s) 753
Eco47I GGWCC 2 cut(s) 245, 1386
Eco57I CTGAAG 3 cut(s) 351, 975, 1114
Eco81I CCTNAGG 1 cut(s) 494
Eco91I GGTNACC 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 1386
EcoO65I GGTNACC 1 cut(s) 40
EcoRII CCWGG 4 cut(s) 45, 357, 1388, 1485
EcoT14I CCWWGG 1 cut(s) 24
EcoT22I ATGCAT 1 cut(s) 371
EcoT38I GRGCYC 1 cut(s) 753
ErhI CCWWGG 1 cut(s) 24
Esp3I CGTCTC 1 cut(s) 1272
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FauI CCCGC 1 cut(s) 953
FauNDI CATATG 1 cut(s) 1189
FbaI TGATCA 1 cut(s) 1497
FblI GTMKAC 1 cut(s) 1312
Fnu4HI GCNGC 2 cut(s) 472, 1491
FokI GGATG 4 cut(s) 71, 227, 869, 900
FriOI GRGCYC 1 cut(s) 753
Fsp4HI GCNGC 2 cut(s) 472, 1491
FspBI CTAG 3 cut(s) 593, 1316, 1461
GlaI GCGC 1 cut(s) 982
GluI GCNGC 2 cut(s) 472, 1491
GsaI CCCAGC 1 cut(s) 830
HaeIII GGCC 3 cut(s) 655, 1448, 1490
HapII CCGG 4 cut(s) 188, 548, 842, 887
HgaI GACGC 3 cut(s) 314, 1118, 1168
HhaI GCGC 1 cut(s) 983
Hin6I GCGC 1 cut(s) 981
HinP1I GCGC 1 cut(s) 981
HpaII CCGG 4 cut(s) 188, 548, 842, 887
HphI GGTGA 4 cut(s) 100, 130, 748, 1532
Hpy166II GTNNAC 2 cut(s) 101, 1313
Hpy188I TCNGA 5 cut(s) 10, 544, 1016, 1068, 1401
Hpy8I GTNNAC 2 cut(s) 101, 1313
Hpy99I CGWCG 1 cut(s) 1367
HpyAV CCTTC 4 cut(s) 76, 628, 736, 1428
HpyCH4III ACNGT 4 cut(s) 112, 126, 1169, 1268
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 9 cut(s) 174, 275, 369, 432, 449, 786, 808, 1158, 1466
HpyF10VI GCNNNNNNNGC 1 cut(s) 783
HpyF3I CTNAG 3 cut(s) 494, 707, 1032
HpySE526I ACGT 1 cut(s) 65
HspAI GCGC 1 cut(s) 981
Kpn2I TCCGGA 2 cut(s) 187, 886
Ksp22I TGATCA 1 cut(s) 1497
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 1 cut(s) 835
Lsp1109I GCAGC 1 cut(s) 458
LweI GCATC 6 cut(s) 49, 183, 205, 334, 378, 634
MaeI CTAG 3 cut(s) 593, 1316, 1461
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 4 cut(s) 40, 282, 299, 1180
MfeI CAATTG 2 cut(s) 240, 427
MflI RGATCY 2 cut(s) 612, 1437
MhlI GDGCHC 3 cut(s) 753, 779, 1266
MlyI GAGTC 4 cut(s) 29, 695, 1007, 1216
Mph1103I ATGCAT 1 cut(s) 371
MroI TCCGGA 2 cut(s) 187, 886
MseI TTAA 5 cut(s) 318, 554, 861, 1164, 1410
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 4 cut(s) 188, 548, 842, 887
MspR9I CCNGG 4 cut(s) 47, 359, 1390, 1487
MunI CAATTG 2 cut(s) 240, 427
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 4 cut(s) 47, 359, 1390, 1487
MwoI GCNNNNNNNGC 1 cut(s) 783
NdeI CATATG 1 cut(s) 1189
NlaIV GGNNCC 1 cut(s) 1439
NmuCI GTSAC 1 cut(s) 1180
NsiI ATGCAT 1 cut(s) 371
NspV TTCGAA 1 cut(s) 986
PagI TCATGA 3 cut(s) 663, 1105, 1200
PceI AGGCCT 1 cut(s) 655
PciSI GCTCTTC 1 cut(s) 741
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 7 cut(s) 400, 634, 872, 920, 941, 969, 1005
PkrI GCNGC 2 cut(s) 473, 1492
PleI GAGTC 4 cut(s) 28, 694, 1006, 1215
PpsI GAGTC 4 cut(s) 28, 694, 1006, 1215
Ppu21I YACGTR 1 cut(s) 66
PpuMI RGGWCCY 1 cut(s) 1386
Psp5II RGGWCCY 1 cut(s) 1386
Psp6I CCWGG 4 cut(s) 45, 357, 1388, 1485
PspEI GGTNACC 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 826
PspGI CCWGG 4 cut(s) 45, 357, 1388, 1485
PspN4I GGNNCC 1 cut(s) 1439
PspPI GGNCC 3 cut(s) 245, 1386, 1446
PspPPI RGGWCCY 1 cut(s) 1386
PstNI CAGNNNCTG 1 cut(s) 1389
PsuI RGATCY 2 cut(s) 612, 1437
RsaI GTAC 3 cut(s) 1030, 1171, 1394
RsaNI GTAC 3 cut(s) 1029, 1170, 1393
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 5 cut(s) 318, 554, 861, 1164, 1410
SatI GCNGC 2 cut(s) 472, 1491
Sau96I GGNCC 3 cut(s) 245, 1386, 1446
ScaI AGTACT 1 cut(s) 1030
SchI GAGTC 4 cut(s) 29, 695, 1007, 1216
ScrFI CCNGG 4 cut(s) 47, 359, 1390, 1487
SduI GDGCHC 3 cut(s) 753, 779, 1266
SfaNI GCATC 6 cut(s) 49, 183, 205, 334, 378, 634
SfcI CTRYAG 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 986
SinI GGWCC 2 cut(s) 245, 1386
SmlI CTYRAG 3 cut(s) 581, 629, 1241
SmoI CTYRAG 3 cut(s) 581, 629, 1241
SseBI AGGCCT 1 cut(s) 655
SsiI CCGC 3 cut(s) 164, 946, 1491
SspMI CTAG 3 cut(s) 593, 1316, 1461
StuI AGGCCT 1 cut(s) 655
StyD4I CCNGG 4 cut(s) 45, 357, 1388, 1485
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 4 cut(s) 112, 126, 1169, 1268
TaiI ACGT 1 cut(s) 68
TaqI TCGA 5 cut(s) 131, 372, 798, 986, 1008
TaqII GACCGA 1 cut(s) 679
TatI WGTACW 1 cut(s) 1028
TauI GCSGC 1 cut(s) 1493
TfiI GAWTC 7 cut(s) 400, 634, 872, 920, 941, 969, 1005
Tru1I TTAA 5 cut(s) 318, 554, 861, 1164, 1410
Tru9I TTAA 5 cut(s) 318, 554, 861, 1164, 1410
TscAI CASTG 3 cut(s) 117, 913, 1230
TseFI GTSAC 1 cut(s) 1180
TseI GCWGC 1 cut(s) 471
Tsp45I GTSAC 1 cut(s) 1180
TspDTI ATGAA 6 cut(s) 371, 466, 536, 680, 1094, 1389
TspRI CASTG 3 cut(s) 117, 913, 1230
VpaK11BI GGWCC 2 cut(s) 245, 1386
XapI RAATTY 2 cut(s) 812, 1301
XmiI GTMKAC 1 cut(s) 1312
XspI CTAG 3 cut(s) 593, 1316, 1461
ZrmI AGTACT 1 cut(s) 1030
Zsp2I ATGCAT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.