Prupe.6G274200_v2.0.a1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000731
Physical Location & Seq
Forward (+)
25904997 .. 25907682
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G274200.1

Sequence Viewer

Length: 1431 bp
ATGTCTCAATCAGAAGTGAAAAAGGATCAACAGCTGGAAGACAAAGAGATCCCAACGAAAGCTACGTTGATGGAGGAGATCAGACAGAGAATGGGAGGAGTGAAGAGAACAGTGGACGTATGCATCTATAAAGTTCCAAGTAAACTTCGAAAAGTGAATGAGGATGCCTACAATCCACGTGTCGTCTCAATTGGACCTTACCATCAAGAAAACCCTGACCTTGTAGATGCTATGAAAGAGCATAAATGGCGTTACATGACAAGCTTTCTTCAGCAAACAAATATTCCTCAAGAGTCTAAGGAATGCTTGGAACGCTGTGTTGATGCTATTTATGAGTTATGTGGCGAGGCTCGTCAATGCTACTCCGAAACTATCAAATACAACGAGAATGAGCTCGCAAAAATAATGTTACTTGACGGTTGCTTCATATTGGAACTTTTCGTCAGGTGCCATGCCAATGTAGAAGTAAACGAAGATGGCCAACCTGATCCAGTACGAAAGTCTGCTTGGATGATCACAGCTCTTCAGCATGACCTGGGATTACTTGAGAACCAGATCCCCTTCTTTATTCTGGTTCGTTTGTATGAGATTGTCAAGCCTCGTGCCACGAAGAATTACTCCGTCGCTAGCCTTGCTCTCAAGTTCTTTGACCCTTTGAGCCGAAAGCCAAGACCTGAGGAAAAGGATCAGCTGGGCACAGACTTCAAGCATTTACTTGATCTCTTGCACAAATTCTACTTCCTCACAGCTGTATCAGTACCTAGTGTCGTGCTCAACAATATTACTGAGGAACAAACTAGTTCATGCCCTTCTGAACCTGATTATACATCGAGCAACAAGTGGGGATTCAAGTATTGCACTTCGGAACTTTTGGAGGCTGCAATCGAGTTTCAAATTCCGGCTTGGGACGACAACAAATTGTTGAACATAAATTTCGACAATGGAGTCCTGAGCATTCCCCAATTGATCATTACCGACGCATCGAGTTCAGTGTTGAGGAACCTCATTGCCTTCGAGCAGTACGGTGTTAGCAGTACAAATGGAGTTACATCGTACGCATTCCTTCTGCAGAGTCTCATAAGTAACACATCAGATTTCAAGCTGCTTCTGGACAAAGAGATCATAGCCCATAATCGGTTTAGCGACCCTGAATTTTTGAGTGAATTTGAGACTATTGTAAAGGATGTTGTTCCCAAGGACGACTTCTACTTTGCTAAATTGCGGGACCAAGTGGACAAATACAAGACGCCCTGGTACCATTTTAACAAGCTTAAAGTATTTTTTGTGGTGCAACTTCAAAGTGAAATTATGATCGTTTGGAAGAATCGTTTCACTGCTAAATGGAGTTTCTTATCAGTGCTGAGTGCGCTTGCCCTTCTCATTCTCGCTTTCTTACAGACATATTATACCATGCACGACCCTTCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

477

Amino Acids

55.02

Weight (kDa)

5.47

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 944
Acc65I GGTACC 1 cut(s) 1254
AccB1I GGYRCC 2 cut(s) 447, 1254
AciI CCGC 1 cut(s) 1222
AclWI GGATC 5 cut(s) 33, 43, 482, 550, 693
AcoI YGGCCR 1 cut(s) 478
AcsI RAATTY 5 cut(s) 731, 894, 931, 1151, 1163
AcuI CTGAAG 2 cut(s) 254, 509
AcvI CACGTG 1 cut(s) 179
AcyI GRCGYC 1 cut(s) 1247
AfaI GTAC 6 cut(s) 495, 759, 1022, 1036, 1055, 1256
AfiI CCNNNNNNNGG 1 cut(s) 1134
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 6 cut(s) 706, 850, 893, 925, 1099, 1298
AhlI ACTAGT 1 cut(s) 797
AjnI CCWGG 2 cut(s) 534, 1250
AjuI GAANNNNNNNTTGG 4 cut(s) 490, 522, 1187, 1219
Alw21I GWGCWC 2 cut(s) 396, 774
Alw26I GTCTC 4 cut(s) 9, 190, 1079, 1163
AlwI GGATC 5 cut(s) 33, 43, 482, 550, 693
AoxI GGCC 1 cut(s) 478
ApeKI GCWGC 2 cut(s) 878, 1102
ApoI RAATTY 5 cut(s) 731, 894, 931, 1151, 1163
ArsI GACNNNNNNTTYG 2 cut(s) 1193, 1225
Asp700I GAANNNNTTC 1 cut(s) 1328
Asp718I GGTACC 1 cut(s) 1254
AspLEI GCGC 1 cut(s) 1369
AspS9I GGNCC 2 cut(s) 194, 1225
AsuII TTCGAA 1 cut(s) 148
AsuNHI GCTAGC 1 cut(s) 626
AvaII GGWCC 2 cut(s) 194, 1225
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 1 cut(s) 698
BaeI ACNNNNGTAYC 2 cut(s) 735, 768
BalI TGGCCA 1 cut(s) 480
BanI GGYRCC 2 cut(s) 447, 1254
BanII GRGCYC 1 cut(s) 396
BauI CACGAG 1 cut(s) 600
BbrPI CACGTG 1 cut(s) 179
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 2 cut(s) 396, 774
BbvI GCAGC 2 cut(s) 865, 1089
BccI CCATC 3 cut(s) 64, 210, 470
BciT130I CCWGG 2 cut(s) 536, 1252
BclI TGATCA 2 cut(s) 513, 966
BcoDI GTCTC 4 cut(s) 9, 190, 1079, 1163
BcuI ACTAGT 1 cut(s) 797
BfaI CTAG 3 cut(s) 627, 762, 798
BfmI CTRYAG 1 cut(s) 1067
BisI GCNGC 2 cut(s) 879, 1103
BlsI GCNGC 2 cut(s) 880, 1104
Bme1390I CCNGG 2 cut(s) 536, 1252
Bme18I GGWCC 2 cut(s) 194, 1225
BmgT120I GGNCC 2 cut(s) 194, 1225
BmiI GGNNCC 4 cut(s) 449, 1001, 1226, 1256
BmrFI CCNGG 2 cut(s) 536, 1252
BmsI GCATC 5 cut(s) 132, 154, 217, 313, 989
BmtI GCTAGC 1 cut(s) 630
BpiI GAAGAC 1 cut(s) 45
Bpu10I CCTNAGC 1 cut(s) 950
Bpu14I TTCGAA 1 cut(s) 148
BpuEI CTTGAG 3 cut(s) 273, 566, 623
BsaAI YACGTR 1 cut(s) 179
BsaHI GRCGYC 1 cut(s) 1247
BsaJI CCNNGG 3 cut(s) 535, 1194, 1250
Bsc4I CCNNNNNNNGG 1 cut(s) 1134
Bse1I ACTGG 1 cut(s) 491
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 1 cut(s) 1005
BseBI CCWGG 2 cut(s) 536, 1252
BseDI CCNNGG 3 cut(s) 535, 1194, 1250
BseGI GGATG 3 cut(s) 169, 516, 1189
BseLI CCNNNNNNNGG 1 cut(s) 1134
BseMI GCAATG 1 cut(s) 1005
BseMII CTCAG 4 cut(s) 666, 777, 941, 1352
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 2 cut(s) 89, 111
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 2 cut(s) 865, 1089
BseYI CCCAGC 1 cut(s) 691
BshFI GGCC 1 cut(s) 480
BshNI GGYRCC 2 cut(s) 447, 1254
BsiHKAI GWGCWC 2 cut(s) 396, 774
BsiSI CCGG 1 cut(s) 899
BsiWI CGTACG 1 cut(s) 1053
BslFI GGGAC 2 cut(s) 920, 1238
BslI CCNNNNNNNGG 1 cut(s) 1134
BsmAI GTCTC 4 cut(s) 9, 190, 1079, 1163
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 2 cut(s) 920, 1238
BsmI GAATGC 3 cut(s) 308, 954, 1058
BsnI GGCC 1 cut(s) 480
Bsp119I TTCGAA 1 cut(s) 148
Bsp1286I GDGCHC 3 cut(s) 396, 698, 774
BspACI CCGC 1 cut(s) 1222
BspANI GGCC 1 cut(s) 480
BspCNI CTCAG 4 cut(s) 667, 778, 942, 1353
BspLI GGNNCC 4 cut(s) 449, 1001, 1226, 1256
BspMAI CTGCAG 1 cut(s) 1071
BspOI GCTAGC 1 cut(s) 630
BspPI GGATC 5 cut(s) 33, 43, 482, 550, 693
BspQI GCTCTTC 1 cut(s) 528
BspT104I TTCGAA 1 cut(s) 148
BspT107I GGYRCC 2 cut(s) 447, 1254
BsrDI GCAATG 1 cut(s) 1005
BsrI ACTGG 1 cut(s) 491
BssECI CCNNGG 3 cut(s) 535, 1194, 1250
BssNI GRCGYC 1 cut(s) 1247
BssSI CACGAG 1 cut(s) 600
BssT1I CCWWGG 1 cut(s) 1194
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 536, 1252
Bst4CI ACNGT 3 cut(s) 112, 419, 1025
Bst6I CTCTTC 2 cut(s) 98, 528
BstACI GRCGYC 1 cut(s) 1247
BstBAI YACGTR 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 148
BstC8I GCNNGC 3 cut(s) 396, 628, 1371
BstDEI CTNAG 5 cut(s) 297, 675, 786, 950, 1361
BstF5I GGATG 3 cut(s) 169, 516, 1189
BstHHI GCGC 1 cut(s) 1369
BstMAI GTCTC 4 cut(s) 9, 190, 1079, 1163
BstMWI GCNNNNNNNGC 4 cut(s) 247, 312, 632, 1366
BstNI CCWGG 2 cut(s) 536, 1252
BstSCI CCNGG 2 cut(s) 534, 1250
BstSFI CTRYAG 1 cut(s) 1067
BstSLI GKGCMC 1 cut(s) 698
BstV1I GCAGC 2 cut(s) 865, 1089
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 2 cut(s) 48, 555
BstYI RGATCY 2 cut(s) 48, 555
Bsu36I CCTNAGG 1 cut(s) 675
BsuRI GGCC 1 cut(s) 480
BtsCI GGATG 3 cut(s) 169, 516, 1189
BtsI GCAGTG 1 cut(s) 1332
BtsIMutI CAGTG 4 cut(s) 117, 996, 1332, 1362
Cac8I GCNNGC 3 cut(s) 396, 628, 1371
CfoI GCGC 1 cut(s) 1369
Cfr13I GGNCC 2 cut(s) 194, 1225
CseI GACGC 2 cut(s) 986, 1255
Csp6I GTAC 6 cut(s) 494, 758, 1021, 1035, 1054, 1255
CviAII CATG 5 cut(s) 256, 452, 530, 804, 1411
CviQI GTAC 6 cut(s) 494, 758, 1021, 1035, 1054, 1255
DdeI CTNAG 5 cut(s) 297, 675, 786, 950, 1361
DrdI GACNNNNNNGTC 1 cut(s) 944
DseDI GACNNNNNNGTC 1 cut(s) 944
EaeI YGGCCR 1 cut(s) 478
Eam1104I CTCTTC 2 cut(s) 98, 528
EarI CTCTTC 2 cut(s) 98, 528
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 1194
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 2 cut(s) 194, 1225
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 2 cut(s) 254, 509
Eco72I CACGTG 1 cut(s) 179
Eco81I CCTNAGG 1 cut(s) 675
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 534, 1250
EcoT14I CCWWGG 1 cut(s) 1194
EcoT22I ATGCAT 1 cut(s) 125
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 1194
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 5 cut(s) 259, 455, 533, 807, 1414
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 1187, 1219
FaqI GGGAC 2 cut(s) 920, 1238
FatI CATG 5 cut(s) 255, 451, 529, 803, 1410
FauI CCCGC 1 cut(s) 1215
FbaI TGATCA 2 cut(s) 513, 966
Fnu4HI GCNGC 2 cut(s) 879, 1103
FokI GGATG 3 cut(s) 176, 523, 1196
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 2 cut(s) 879, 1103
FspBI CTAG 3 cut(s) 627, 762, 798
GlaI GCGC 1 cut(s) 1368
GluI GCNGC 2 cut(s) 879, 1103
GsaI CCCAGC 1 cut(s) 695
HaeIII GGCC 1 cut(s) 480
HapII CCGG 1 cut(s) 899
HgaI GACGC 2 cut(s) 986, 1255
HhaI GCGC 1 cut(s) 1369
Hin1I GRCGYC 1 cut(s) 1247
Hin1II CATG 5 cut(s) 259, 455, 533, 807, 1414
Hin6I GCGC 1 cut(s) 1367
HinP1I GCGC 1 cut(s) 1367
HindIII AAGCTT 2 cut(s) 262, 1268
HinfI GANTC 5 cut(s) 293, 846, 945, 1072, 1324
HpaII CCGG 1 cut(s) 899
Hpy166II GTNNAC 4 cut(s) 115, 143, 469, 1234
Hpy188I TCNGA 6 cut(s) 13, 83, 367, 814, 865, 1093
Hpy188III TCNNGA 4 cut(s) 206, 290, 949, 1109
Hpy8I GTNNAC 4 cut(s) 115, 143, 469, 1234
Hpy99I CGWCG 2 cut(s) 626, 980
HpyAV CCTTC 6 cut(s) 571, 819, 1021, 1073, 1385, 1431
HpyCH4III ACNGT 3 cut(s) 112, 419, 1025
HpyCH4IV ACGT 3 cut(s) 65, 117, 178
HpyCH4V TGCA 7 cut(s) 123, 727, 858, 881, 1069, 1291, 1414
HpyF10VI GCNNNNNNNGC 4 cut(s) 247, 312, 632, 1366
HpyF3I CTNAG 5 cut(s) 297, 675, 786, 950, 1361
HpySE526I ACGT 3 cut(s) 65, 117, 178
Hsp92I GRCGYC 1 cut(s) 1247
Hsp92II CATG 5 cut(s) 259, 455, 533, 807, 1414
HspAI GCGC 1 cut(s) 1367
KpnI GGTACC 1 cut(s) 1258
Ksp22I TGATCA 2 cut(s) 513, 966
LguI GCTCTTC 1 cut(s) 528
Lsp1109I GCAGC 2 cut(s) 865, 1089
LweI GCATC 5 cut(s) 132, 154, 217, 313, 989
MaeI CTAG 3 cut(s) 627, 762, 798
MaeII ACGT 3 cut(s) 65, 117, 178
MaeIII GTNAC 4 cut(s) 251, 408, 1045, 1082
MboII GAAGA 7 cut(s) 50, 115, 260, 485, 515, 622, 1333
MfeI CAATTG 2 cut(s) 189, 962
MflI RGATCY 2 cut(s) 48, 555
MhlI GDGCHC 3 cut(s) 396, 698, 774
MlsI TGGCCA 1 cut(s) 480
MluNI TGGCCA 1 cut(s) 480
MlyI GAGTC 3 cut(s) 302, 954, 1081
Mox20I TGGCCA 1 cut(s) 480
Mph1103I ATGCAT 1 cut(s) 125
MroXI GAANNNNTTC 1 cut(s) 1328
MscI TGGCCA 1 cut(s) 480
MseI TTAA 2 cut(s) 1263, 1272
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 1 cut(s) 480
MspA1I CMGCKG 4 cut(s) 34, 691, 749, 1428
MspI CCGG 1 cut(s) 899
MspR9I CCNGG 2 cut(s) 536, 1252
MunI CAATTG 2 cut(s) 189, 962
Mva1269I GAATGC 3 cut(s) 308, 954, 1058
MvaI CCWGG 2 cut(s) 536, 1252
MwoI GCNNNNNNNGC 4 cut(s) 247, 312, 632, 1366
NheI GCTAGC 1 cut(s) 626
NlaIII CATG 5 cut(s) 259, 455, 533, 807, 1414
NlaIV GGNNCC 4 cut(s) 449, 1001, 1226, 1256
NsiI ATGCAT 1 cut(s) 125
NspV TTCGAA 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 528
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PctI GAATGC 3 cut(s) 308, 954, 1058
PdmI GAANNNNTTC 1 cut(s) 1328
PfeI GAWTC 2 cut(s) 846, 1324
Pfl23II CGTACG 1 cut(s) 1053
PkrI GCNGC 2 cut(s) 880, 1104
PleI GAGTC 3 cut(s) 301, 953, 1080
PmaCI CACGTG 1 cut(s) 179
PmlI CACGTG 1 cut(s) 179
PpsI GAGTC 3 cut(s) 301, 953, 1080
Ppu21I YACGTR 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 534, 1250
PspCI CACGTG 1 cut(s) 179
PspFI CCCAGC 1 cut(s) 691
PspGI CCWGG 2 cut(s) 534, 1250
PspLI CGTACG 1 cut(s) 1053
PspN4I GGNNCC 4 cut(s) 449, 1001, 1226, 1256
PspPI GGNCC 2 cut(s) 194, 1225
PstI CTGCAG 1 cut(s) 1071
PsuI RGATCY 2 cut(s) 48, 555
PvuII CAGCTG 4 cut(s) 34, 691, 749, 1428
RsaI GTAC 6 cut(s) 495, 759, 1022, 1036, 1055, 1256
RsaNI GTAC 6 cut(s) 494, 758, 1021, 1035, 1054, 1255
RseI CAYNNNNRTG 1 cut(s) 456
SacI GAGCTC 1 cut(s) 396
SapI GCTCTTC 1 cut(s) 528
SaqAI TTAA 2 cut(s) 1263, 1272
SatI GCNGC 2 cut(s) 879, 1103
Sau96I GGNCC 2 cut(s) 194, 1225
SchI GAGTC 3 cut(s) 302, 954, 1081
ScrFI CCNGG 2 cut(s) 536, 1252
SduI GDGCHC 3 cut(s) 396, 698, 774
SfaNI GCATC 5 cut(s) 132, 154, 217, 313, 989
SfcI CTRYAG 1 cut(s) 1067
SfuI TTCGAA 1 cut(s) 148
SinI GGWCC 2 cut(s) 194, 1225
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 3 cut(s) 288, 545, 638
SmoI CTYRAG 3 cut(s) 288, 545, 638
SpeI ACTAGT 1 cut(s) 797
SsiI CCGC 1 cut(s) 1222
SspI AATATT 2 cut(s) 283, 781
SspMI CTAG 3 cut(s) 627, 762, 798
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 534, 1250
StyI CCWWGG 1 cut(s) 1194
TaaI ACNGT 3 cut(s) 112, 419, 1025
TaiI ACGT 3 cut(s) 68, 120, 181
TaqI TCGA 6 cut(s) 148, 830, 885, 936, 983, 1014
TatI WGTACW 1 cut(s) 1034
TfiI GAWTC 2 cut(s) 846, 1324
Tru1I TTAA 2 cut(s) 1263, 1272
Tru9I TTAA 2 cut(s) 1263, 1272
TscAI CASTG 4 cut(s) 117, 996, 1339, 1362
TseI GCWGC 2 cut(s) 878, 1102
TspDTI ATGAA 3 cut(s) 248, 415, 792
TspGWI ACGGA 1 cut(s) 610
TspRI CASTG 4 cut(s) 117, 996, 1339, 1362
VpaK11BI GGWCC 2 cut(s) 194, 1225
XapI RAATTY 5 cut(s) 731, 894, 931, 1151, 1163
XmnI GAANNNNTTC 1 cut(s) 1328
XspI CTAG 3 cut(s) 627, 762, 798
Zsp2I ATGCAT 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.