Rh5AG131400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000731
Physical Location & Seq
Forward (+)
13215587 .. 13217378
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG131400.1

Sequence Viewer

Length: 753 bp
ATGTTTGGTCTGTGTAATCAATTTGGTTGGGTTGGTTGGTGGGTTGCTCACGCATCAGAGTTTGTGTGGGTGCTGCTCATTGCTAGGTCTAATTACGGGTTCGTTTTGGATTTGGAATTTAGGCGAATGGAAAAAGACTTGGAAGAGAAGCTGAAACAGCTCATTGACCAGGGGATTAAAGTTGAGATCGTCAAAGATGGCAACCAGCCAAGAGGCACGTACACTATAGAAGTACATCCCCACACTTTGGAGGTGATGGAAGTTTGGGGCAAGCAGATGAAGATTTTAGGGATGCTGAGTGATCTATACATTCTTGCTGAAGTCAAGTTGACTTTGAATGAGTCCGGTTTGTTGGCATCCTTGTACCAGCACGGCCCAATTGCCACCTGGAGAGCAGTAGCTCAGAGAATGAAAGGGAAAGCTATTGATTCTGCTCATGAGCTAAAGATAACAGAGACATGGCTAGATAGGATTGGACCATTGGCCAAAGAAGTCTCTACTGTTCTCATCGTTCATCAGAAACAAAAGAAGACAGCCTCACAACCTGAAGAAGGTAGCTTAGAGGTCCTCCCCCATGACTTTGGATCCATTTTGATCAAACTTTCCCAACTTCGTTTGAAATGGAAGGTCATTGCTTCTTTGGAGAGTGTAACACTAATGGATGACTCTGAGATGGAAGACATGAATGAGATAGAATCGATAATCAGAGATGTACACAAGGTTGTGGTTGTCCAACAACTGTTGCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

28.6

Weight (kDa)

5.93

Isoelectric Point (pI)

28.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 247
AclWI GGATC 2 cut(s) 579, 592
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 1 cut(s) 116
AcuI CTGAAG 2 cut(s) 339, 567
AfaI GTAC 4 cut(s) 221, 234, 365, 714
AfiI CCNNNNNNNGG 2 cut(s) 247, 551
AgsI TTSAA 2 cut(s) 337, 619
AjnI CCWGG 2 cut(s) 168, 386
AluBI AGCT 6 cut(s) 151, 160, 401, 422, 442, 558
AluI AGCT 6 cut(s) 151, 160, 401, 422, 442, 558
Alw26I GTCTC 2 cut(s) 449, 499
AlwI GGATC 2 cut(s) 579, 592
AoxI GGCC 2 cut(s) 373, 483
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 1 cut(s) 116
AspS9I GGNCC 3 cut(s) 374, 476, 565
AsuHPI GGTGA 1 cut(s) 265
AvaII GGWCC 2 cut(s) 476, 565
BalI TGGCCA 1 cut(s) 485
BamHI GGATCC 1 cut(s) 584
BbsI GAAGAC 2 cut(s) 536, 684
BbvI GCAGC 1 cut(s) 60
BccI CCATC 3 cut(s) 191, 250, 667
BceAI ACGGC 1 cut(s) 388
BciT130I CCWGG 2 cut(s) 170, 388
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 449, 499
BfaI CTAG 2 cut(s) 84, 464
BfmI CTRYAG 1 cut(s) 225
BisI GCNGC 1 cut(s) 74
BlsI GCNGC 1 cut(s) 75
Bme1390I CCNGG 2 cut(s) 170, 388
Bme18I GGWCC 2 cut(s) 476, 565
BmgT120I GGNCC 3 cut(s) 374, 476, 565
BmiI GGNNCC 1 cut(s) 586
BmrFI CCNGG 2 cut(s) 170, 388
BmsI GCATC 3 cut(s) 62, 282, 365
BpiI GAAGAC 2 cut(s) 536, 684
BpmI CTGGAG 1 cut(s) 409
Bsa29I ATCGAT 1 cut(s) 698
BsaAI YACGTR 1 cut(s) 219
BsaJI CCNNGG 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 551
Bse3DI GCAATG 2 cut(s) 78, 630
BseBI CCWGG 2 cut(s) 170, 388
BseCI ATCGAT 1 cut(s) 698
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 4 cut(s) 235, 297, 356, 667
BseLI CCNNNNNNNGG 2 cut(s) 247, 551
BseMI GCAATG 2 cut(s) 78, 630
BseMII CTCAG 3 cut(s) 287, 416, 660
BseXI GCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 375, 485
BshVI ATCGAT 1 cut(s) 698
BsiSI CCGG 1 cut(s) 345
BslI CCNNNNNNNGG 2 cut(s) 247, 551
BsmAI GTCTC 2 cut(s) 449, 499
BsnI GGCC 2 cut(s) 375, 485
Bsp1407I TGTACA 1 cut(s) 712
Bsp143I GATC 4 cut(s) 186, 301, 584, 594
BspANI GGCC 2 cut(s) 375, 485
BspCNI CTCAG 3 cut(s) 288, 415, 661
BspDI ATCGAT 1 cut(s) 698
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 1 cut(s) 586
BspPI GGATC 2 cut(s) 579, 592
BsrDI GCAATG 2 cut(s) 78, 630
BsrGI TGTACA 1 cut(s) 712
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 4 cut(s) 186, 301, 584, 594
Bst2UI CCWGG 2 cut(s) 170, 388
Bst4CI ACNGT 2 cut(s) 502, 741
Bst6I CTCTTC 1 cut(s) 138
BstAUI TGTACA 1 cut(s) 712
BstBAI YACGTR 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 272
BstDEI CTNAG 4 cut(s) 296, 402, 559, 669
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 4 cut(s) 235, 297, 356, 667
BstKTI GATC 4 cut(s) 189, 304, 587, 597
BstMAI GTCTC 2 cut(s) 449, 499
BstMBI GATC 4 cut(s) 186, 301, 584, 594
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 2 cut(s) 170, 388
BstSCI CCNGG 2 cut(s) 168, 386
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 60
BstV2I GAAGAC 2 cut(s) 536, 684
BstX2I RGATCY 1 cut(s) 584
BstXI CCANNNNNNTGG 1 cut(s) 581
BstYI RGATCY 1 cut(s) 584
Bsu15I ATCGAT 1 cut(s) 698
BsuRI GGCC 2 cut(s) 375, 485
BsuTUI ATCGAT 1 cut(s) 698
BtsCI GGATG 4 cut(s) 235, 297, 356, 667
Cac8I GCNNGC 1 cut(s) 272
CciI TCATGA 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 374, 476, 565
ClaI ATCGAT 1 cut(s) 698
Csp6I GTAC 4 cut(s) 220, 233, 364, 713
CviAII CATG 4 cut(s) 437, 459, 575, 682
CviQI GTAC 4 cut(s) 220, 233, 364, 713
DdeI CTNAG 4 cut(s) 296, 402, 559, 669
DpnI GATC 4 cut(s) 188, 303, 586, 596
DpnII GATC 4 cut(s) 186, 301, 584, 594
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 476, 565
Eco57I CTGAAG 2 cut(s) 339, 567
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 565
EcoRII CCWGG 2 cut(s) 168, 386
FaeI CATG 4 cut(s) 440, 462, 578, 685
FaiI YATR 6 cut(s) 227, 307, 438, 460, 576, 683
FatI CATG 4 cut(s) 436, 458, 574, 681
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 1 cut(s) 74
FokI GGATG 4 cut(s) 222, 304, 343, 674
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 2 cut(s) 84, 464
GluI GCNGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 409
HaeIII GGCC 2 cut(s) 375, 485
HapII CCGG 1 cut(s) 345
Hin1II CATG 4 cut(s) 440, 462, 578, 685
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 4 cut(s) 341, 428, 665, 695
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 3 cut(s) 222, 330, 715
Hpy188I TCNGA 5 cut(s) 58, 405, 519, 670, 707
Hpy188III TCNNGA 1 cut(s) 437
Hpy8I GTNNAC 3 cut(s) 222, 330, 715
HpyAV CCTTC 2 cut(s) 545, 619
HpyCH4III ACNGT 2 cut(s) 502, 741
HpyCH4IV ACGT 1 cut(s) 218
HpyCH4V TGCA 1 cut(s) 745
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 4 cut(s) 296, 402, 559, 669
HpySE526I ACGT 1 cut(s) 218
Hsp92II CATG 4 cut(s) 440, 462, 578, 685
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 4 cut(s) 186, 301, 584, 594
LpnPI CCDG 8 cut(s) 155, 182, 218, 358, 373, 380, 400, 558
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 3 cut(s) 62, 282, 365
MaeI CTAG 2 cut(s) 84, 464
MaeII ACGT 1 cut(s) 218
MaeIII GTNAC 1 cut(s) 649
MalI GATC 4 cut(s) 188, 303, 586, 596
MboI GATC 4 cut(s) 186, 301, 584, 594
MboII GAAGA 5 cut(s) 155, 292, 541, 560, 689
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 1 cut(s) 584
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 4 cut(s) 20, 91, 116, 378
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 350, 659
MnlI CCTC 5 cut(s) 206, 244, 547, 556, 578
Mox20I TGGCCA 1 cut(s) 485
MscI TGGCCA 1 cut(s) 485
MseI TTAA 1 cut(s) 177
Msp20I TGGCCA 1 cut(s) 485
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 170, 388
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 2 cut(s) 170, 388
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 4 cut(s) 186, 301, 584, 594
NlaIII CATG 4 cut(s) 440, 462, 578, 685
NlaIV GGNNCC 1 cut(s) 586
PagI TCATGA 1 cut(s) 436
PfeI GAWTC 2 cut(s) 428, 695
PflMI CCANNNNNTGG 1 cut(s) 247
PkrI GCNGC 1 cut(s) 75
PleI GAGTC 2 cut(s) 349, 659
PpsI GAGTC 2 cut(s) 349, 659
Ppu21I YACGTR 1 cut(s) 219
PpuMI RGGWCCY 1 cut(s) 565
Psp5II RGGWCCY 1 cut(s) 565
Psp6I CCWGG 2 cut(s) 168, 386
PspGI CCWGG 2 cut(s) 168, 386
PspN4I GGNNCC 1 cut(s) 586
PspPI GGNCC 3 cut(s) 374, 476, 565
PspPPI RGGWCCY 1 cut(s) 565
PsuI RGATCY 1 cut(s) 584
RsaI GTAC 4 cut(s) 221, 234, 365, 714
RsaNI GTAC 4 cut(s) 220, 233, 364, 713
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 4 cut(s) 186, 301, 584, 594
Sau96I GGNCC 3 cut(s) 374, 476, 565
SchI GAGTC 2 cut(s) 350, 659
ScrFI CCNGG 2 cut(s) 170, 388
SfaNI GCATC 3 cut(s) 62, 282, 365
SfcI CTRYAG 1 cut(s) 225
SinI GGWCC 2 cut(s) 476, 565
Sse9I AATT 4 cut(s) 20, 91, 116, 378
SspMI CTAG 2 cut(s) 84, 464
StyD4I CCNGG 2 cut(s) 168, 386
TaaI ACNGT 2 cut(s) 502, 741
TaiI ACGT 1 cut(s) 221
TaqI TCGA 1 cut(s) 698
TasI AATT 4 cut(s) 20, 91, 116, 378
TatI WGTACW 2 cut(s) 232, 712
TfiI GAWTC 2 cut(s) 428, 695
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TseI GCWGC 1 cut(s) 73
TspDTI ATGAA 4 cut(s) 293, 425, 503, 698
Van91I CCANNNNNTGG 1 cut(s) 247
VpaK11BI GGWCC 2 cut(s) 476, 565
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 1 cut(s) 116
XcmI CCANNNNNNNNNTGG 1 cut(s) 384
XspI CTAG 2 cut(s) 84, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.