RLG00000023552

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000731
Physical Location & Seq
Forward (+)
25160254 .. 25161920
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023552

Sequence Viewer

Length: 1431 bp
ATGTATCGAAAGAAACTTAAGTGGACCACCGAAATCGAAGAAAAGCTCCACAATGTGCGGCCGTGGAACTTGGGCATCTACAGAGTTCCAAATAGACTTCGTCAAGTCAATGAGGATGCCTACAGCCCTCATGTTGTCTGCATCGGTCCTTTCCATCGACACAAGCCGAACCTAGTAGCTATGAGAGAGCACAAGTCGCGTTATGCGCTATACTTCTTTAAGCAAGCACAAGAAATAGCACAAAAACGAGCTCTAAGTTTCAGCTTTGGATCGCGAGAGTCTTCTGAATGCATGAGAGAGTGGGCGACTGCTATTTATGATTCGGACGCAGAGGTTCGTGGAAGCTACGCTGAAGATATGAGCAACATTGATCAGGAGGAACTAGCAGAAATAATGTTGGTCGATGGTTGCTTCTTATTAGAACTTTTCATCAGGTTTTACCTATACGTGCACGGGAAGTTAGAAGGCCCTGAATCTGATCCAATTCTAGAAAGTGCTTGGATGATTGCAGATCTACGGCACGATCTGGCATTGCTTGAAAACCAAATCCCCTTATTTATTATACTTGATTTGTATGATGCTATCAGTGGTCATCAGATTCTTGTCGACAATGATTTGCCTCCGCCACATGGTCTTGCTCTTCATTTCTTTCAACCTGTGAGCCTCAAGGCAAGCATTGTAACAGAGTGGAGACATGAGCACAAGCATTTACTGGATATCTTGCACAAGTTCTATTTCCTTCCGACTAAAACTACGGACTTGTCTATGAGGCTCAATATGGAATTGATCCCTGAGGAGCAGCGTCTTAGGGCAGTCCAAAAGAATAAAGTATGGGGATTCAATTATTGTGCCTCCGAACTTTTGGAATCTGGAGTCGAGTTTGGTATTGGCTCCAGCCAGGACCAGTTGCTGGACATAACTTTCCGTGAGGGAGTAATCAGAATTCCACCACTGTTTATCCATGAGACGACGAGTTCATTGTTGAGGAACCTGATTGCTTATGAGCAATGCAGTCTTAGTAGTACGCATGGCGTTACATCATATGCCTGCCTCATGAAGAGTCTCATCGATTCCTCGCGGGATAGCAATCTGCTTCAGGAGAAGGGGATAATCAAATATGACCATTGGATTGGAGGTGAAGAGGAATATTTGACTCAAATATTCAAAGGTATACTAGATGAAGTTGTTGTCAAGGAATTCCATTTTAGAACCTTGTGCGATGAAGTGAATGAGTACTGTGCATCCTGGTTTCATCTGAGAAAACTCAAAGTATTTCTGAGGGTGCGGATTCAAAGATATATGAGGCTTCTTTTCAGCACTTATTTTTCTTCTCCGTGGAGTTTCATATCATTCATGGCGGCGCTCACTATTCTCATTCTCACATTTTTTCAGACATACTATTCCATGCACACAGCTCGTACTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

477

Amino Acids

55.73

Weight (kDa)

6.14

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 26 - 460 1e-99 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 910
AccI GTMKAC 2 cut(s) 606, 1171
AccII CGCG 3 cut(s) 199, 274, 1078
AciI CCGC 5 cut(s) 58, 623, 1078, 1285, 1358
AclWI GGATC 3 cut(s) 277, 473, 781
AcoI YGGCCR 1 cut(s) 59
AcsI RAATTY 2 cut(s) 942, 1196
AcuI CTGAAG 2 cut(s) 372, 1079
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 3 cut(s) 1024, 1235, 1420
AfiI CCNNNNNNNGG 2 cut(s) 629, 910
AflII CTTAAG 1 cut(s) 17
AgsI TTSAA 5 cut(s) 539, 653, 841, 1165, 1292
AjnI CCWGG 2 cut(s) 897, 1244
AluBI AGCT 6 cut(s) 46, 179, 251, 264, 345, 1415
AluI AGCT 6 cut(s) 46, 179, 251, 264, 345, 1415
Alw21I GWGCWC 4 cut(s) 192, 253, 453, 702
Alw26I GTCTC 3 cut(s) 685, 959, 1067
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 3 cut(s) 277, 473, 781
AlwNI CAGNNNCTG 1 cut(s) 910
AoxI GGCC 2 cut(s) 59, 466
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 1 cut(s) 799
ApoI RAATTY 2 cut(s) 942, 1196
AseI ATTAAT 1 cut(s) 1425
AspLEI GCGC 2 cut(s) 208, 1363
AspS9I GGNCC 4 cut(s) 24, 146, 467, 901
AsuHPI GGTGA 1 cut(s) 1148
AvaII GGWCC 3 cut(s) 24, 146, 901
AxyI CCTNAGG 1 cut(s) 792
BaeGI GKGCMC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 4 cut(s) 192, 253, 453, 702
BbvI GCAGC 1 cut(s) 811
BccI CCATC 2 cut(s) 162, 398
BceAI ACGGC 2 cut(s) 46, 533
BcgI CGANNNNNNTGC 2 cut(s) 1397, 1431
BciT130I CCWGG 2 cut(s) 899, 1246
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 3 cut(s) 685, 959, 1067
BfaI CTAG 4 cut(s) 173, 383, 488, 1175
BfmI CTRYAG 2 cut(s) 79, 121
BfoI RGCGCY 1 cut(s) 1364
BfrI CTTAAG 1 cut(s) 17
BglII AGATCT 1 cut(s) 511
BisI GCNGC 3 cut(s) 59, 800, 1359
BlsI GCNGC 3 cut(s) 60, 801, 1360
BmcAI AGTACT 1 cut(s) 1235
Bme1390I CCNGG 2 cut(s) 899, 1246
Bme18I GGWCC 3 cut(s) 24, 146, 901
BmgT120I GGNCC 4 cut(s) 24, 146, 467, 901
BmiI GGNNCC 2 cut(s) 892, 989
BmrFI CCNGG 2 cut(s) 899, 1246
BmsI GCATC 5 cut(s) 84, 106, 150, 568, 1250
BpiI GAAGAC 1 cut(s) 273
BpmI CTGGAG 2 cut(s) 877, 891
BpuEI CTTGAG 1 cut(s) 650
Bsa29I ATCGAT 1 cut(s) 1068
BsaAI YACGTR 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 1086
BsaJI CCNNGG 2 cut(s) 62, 1334
Bsc4I CCNNNNNNNGG 2 cut(s) 629, 910
Bse1I ACTGG 2 cut(s) 717, 904
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 2 cut(s) 530, 1013
Bse8I GATNNNNATC 1 cut(s) 1086
BseBI CCWGG 2 cut(s) 899, 1246
BseCI ATCGAT 1 cut(s) 1068
BseDI CCNNGG 2 cut(s) 62, 1334
BseGI GGATG 3 cut(s) 121, 507, 1241
BseJI GATNNNNATC 1 cut(s) 1086
BseLI CCNNNNNNNGG 2 cut(s) 629, 910
BseMI GCAATG 2 cut(s) 530, 1013
BseMII CTCAG 3 cut(s) 783, 1247, 1268
BseNI ACTGG 2 cut(s) 717, 904
BseRI GAGGAG 1 cut(s) 809
BseSI GKGCMC 1 cut(s) 453
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 1 cut(s) 811
Bsh1236I CGCG 3 cut(s) 199, 274, 1078
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 2 cut(s) 61, 468
BshVI ATCGAT 1 cut(s) 1068
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 4 cut(s) 192, 253, 453, 702
BslI CCNNNNNNNGG 2 cut(s) 629, 910
BsmAI GTCTC 3 cut(s) 685, 959, 1067
BsmBI CGTCTC 1 cut(s) 959
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 2 cut(s) 61, 468
Bsp1286I GDGCHC 4 cut(s) 192, 253, 453, 702
Bsp143I GATC 6 cut(s) 269, 370, 478, 511, 523, 786
Bsp68I TCGCGA 1 cut(s) 274
BspACI CCGC 5 cut(s) 58, 623, 1078, 1285, 1358
BspANI GGCC 2 cut(s) 61, 468
BspCNI CTCAG 3 cut(s) 784, 1248, 1269
BspDI ATCGAT 1 cut(s) 1068
BspFNI CGCG 3 cut(s) 199, 274, 1078
BspHI TCATGA 1 cut(s) 1053
BspLI GGNNCC 2 cut(s) 892, 989
BspPI GGATC 3 cut(s) 277, 473, 781
BspQI GCTCTTC 1 cut(s) 645
BspTI CTTAAG 1 cut(s) 17
BsrDI GCAATG 2 cut(s) 530, 1013
BsrI ACTGG 2 cut(s) 717, 904
BssECI CCNNGG 2 cut(s) 62, 1334
BssMI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
BssNAI GTATAC 1 cut(s) 1172
Bst1107I GTATAC 1 cut(s) 1172
Bst2UI CCWGG 2 cut(s) 899, 1246
Bst4CI ACNGT 2 cut(s) 954, 1238
Bst6I CTCTTC 3 cut(s) 645, 1052, 1134
BstAFI CTTAAG 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 3 cut(s) 225, 673, 1048
BstDEI CTNAG 6 cut(s) 254, 792, 806, 1016, 1256, 1277
BstDSI CCRYGG 2 cut(s) 62, 1334
BstF5I GGATG 3 cut(s) 121, 507, 1241
BstFNI CGCG 3 cut(s) 199, 274, 1078
BstH2I RGCGCY 1 cut(s) 1364
BstHHI GCGC 2 cut(s) 208, 1363
BstKTI GATC 6 cut(s) 272, 373, 481, 514, 526, 789
BstMAI GTCTC 3 cut(s) 685, 959, 1067
BstMBI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 196, 205
BstNI CCWGG 2 cut(s) 899, 1246
BstSCI CCNGG 2 cut(s) 897, 1244
BstSFI CTRYAG 2 cut(s) 79, 121
BstSLI GKGCMC 1 cut(s) 453
BstUI CGCG 3 cut(s) 199, 274, 1078
BstV1I GCAGC 1 cut(s) 811
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 511
BstXI CCANNNNNNTGG 1 cut(s) 1130
BstYI RGATCY 1 cut(s) 511
BstZ17I GTATAC 1 cut(s) 1172
BstZI CGGCCG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 1068
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 2 cut(s) 61, 468
BsuTUI ATCGAT 1 cut(s) 1068
BtgI CCRYGG 2 cut(s) 62, 1334
BtgZI GCGATG 1 cut(s) 1233
BtsCI GGATG 3 cut(s) 121, 507, 1241
BtsIMutI CAGTG 2 cut(s) 592, 950
BtuMI TCGCGA 1 cut(s) 274
Cac8I GCNNGC 3 cut(s) 225, 673, 1048
CaiI CAGNNNCTG 1 cut(s) 910
CciI TCATGA 1 cut(s) 1053
CfoI GCGC 2 cut(s) 208, 1363
Cfr13I GGNCC 4 cut(s) 24, 146, 467, 901
ClaI ATCGAT 1 cut(s) 1068
CseI GACGC 2 cut(s) 335, 791
Csp6I GTAC 3 cut(s) 1023, 1234, 1419
CviAII CATG 9 cut(s) 131, 292, 629, 695, 962, 1028, 1054, 1354, 1405
CviQI GTAC 3 cut(s) 1023, 1234, 1419
DdeI CTNAG 6 cut(s) 254, 792, 806, 1016, 1256, 1277
DpnI GATC 6 cut(s) 271, 372, 480, 513, 525, 788
DpnII GATC 6 cut(s) 269, 370, 478, 511, 523, 786
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 59
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 3 cut(s) 645, 1052, 1134
EarI CTCTTC 3 cut(s) 645, 1052, 1134
EciI GGCGGA 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 251
EclXI CGGCCG 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 253
Eco32I GATATC 1 cut(s) 718
Eco47I GGWCC 3 cut(s) 24, 146, 901
Eco52I CGGCCG 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 2 cut(s) 372, 1079
Eco81I CCTNAGG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 467
EcoRI GAATTC 2 cut(s) 942, 1196
EcoRII CCWGG 2 cut(s) 897, 1244
EcoRV GATATC 1 cut(s) 718
EcoT22I ATGCAT 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 959
FaeI CATG 9 cut(s) 134, 295, 632, 698, 965, 1031, 1057, 1357, 1408
FatI CATG 9 cut(s) 130, 291, 628, 694, 961, 1027, 1053, 1353, 1404
FauI CCCGC 1 cut(s) 1071
FauNDI CATATG 1 cut(s) 1042
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 2 cut(s) 606, 1171
Fnu4HI GCNGC 3 cut(s) 59, 800, 1359
FokI GGATG 3 cut(s) 128, 514, 1228
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 3 cut(s) 59, 800, 1359
FspBI CTAG 4 cut(s) 173, 383, 488, 1175
GlaI GCGC 2 cut(s) 207, 1362
GluI GCNGC 3 cut(s) 59, 800, 1359
GsuI CTGGAG 2 cut(s) 877, 891
HaeII RGCGCY 1 cut(s) 1364
HaeIII GGCC 2 cut(s) 61, 468
HgaI GACGC 2 cut(s) 335, 791
HhaI GCGC 2 cut(s) 208, 1363
Hin1II CATG 9 cut(s) 134, 295, 632, 698, 965, 1031, 1057, 1357, 1408
Hin6I GCGC 2 cut(s) 206, 1361
HinP1I GCGC 2 cut(s) 206, 1361
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HphI GGTGA 1 cut(s) 1148
Hpy166II GTNNAC 4 cut(s) 24, 451, 607, 1172
Hpy188III TCNNGA 6 cut(s) 273, 374, 488, 870, 1054, 1097
Hpy8I GTNNAC 4 cut(s) 24, 451, 607, 1172
Hpy99I CGWCG 1 cut(s) 973
HpyAV CCTTC 3 cut(s) 458, 749, 1096
HpyCH4III ACNGT 2 cut(s) 954, 1238
HpyCH4IV ACGT 1 cut(s) 447
HpyCH4V TGCA 8 cut(s) 141, 291, 451, 509, 724, 1011, 1241, 1408
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 205
HpyF3I CTNAG 6 cut(s) 254, 792, 806, 1016, 1256, 1277
HpySE526I ACGT 1 cut(s) 447
Hsp92II CATG 9 cut(s) 134, 295, 632, 698, 965, 1031, 1057, 1357, 1408
HspAI GCGC 2 cut(s) 206, 1361
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 6 cut(s) 269, 370, 478, 511, 523, 786
LguI GCTCTTC 1 cut(s) 645
LmnI GCTCC 3 cut(s) 51, 796, 896
Lsp1109I GCAGC 1 cut(s) 811
LweI GCATC 5 cut(s) 84, 106, 150, 568, 1250
MaeI CTAG 4 cut(s) 173, 383, 488, 1175
MaeII ACGT 1 cut(s) 447
MaeIII GTNAC 2 cut(s) 679, 1033
MalI GATC 6 cut(s) 271, 372, 480, 513, 525, 788
MboI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
MboII GAAGA 7 cut(s) 50, 273, 365, 632, 1069, 1151, 1320
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 4 cut(s) 192, 253, 453, 702
MluCI AATT 6 cut(s) 483, 782, 841, 942, 1196, 1426
MlyI GAGTC 4 cut(s) 287, 882, 1069, 1147
MmeI TCCRAC 1 cut(s) 767
Mph1103I ATGCAT 1 cut(s) 293
MseI TTAA 4 cut(s) 18, 219, 1425, 1429
MspCI CTTAAG 1 cut(s) 17
MspR9I CCNGG 2 cut(s) 899, 1246
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 2 cut(s) 899, 1246
MvnI CGCG 3 cut(s) 199, 274, 1078
MwoI GCNNNNNNNGC 2 cut(s) 196, 205
NdeI CATATG 1 cut(s) 1042
NdeII GATC 6 cut(s) 269, 370, 478, 511, 523, 786
NlaIII CATG 9 cut(s) 134, 295, 632, 698, 965, 1031, 1057, 1357, 1408
NlaIV GGNNCC 2 cut(s) 892, 989
NruI TCGCGA 1 cut(s) 274
NsiI ATGCAT 1 cut(s) 293
PacI TTAATTAA 1 cut(s) 1429
PagI TCATGA 1 cut(s) 1053
PciSI GCTCTTC 1 cut(s) 645
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 7 cut(s) 320, 473, 598, 837, 866, 1070, 1288
PflFI GACNNNGTC 1 cut(s) 99
PflMI CCANNNNNTGG 1 cut(s) 910
PkrI GCNGC 3 cut(s) 60, 801, 1360
PleI GAGTC 4 cut(s) 286, 881, 1068, 1147
PpsI GAGTC 4 cut(s) 286, 881, 1068, 1147
Ppu21I YACGTR 1 cut(s) 448
PshBI ATTAAT 1 cut(s) 1425
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 897, 1244
PspGI CCWGG 2 cut(s) 897, 1244
PspN4I GGNNCC 2 cut(s) 892, 989
PspPI GGNCC 4 cut(s) 24, 146, 467, 901
PstNI CAGNNNCTG 1 cut(s) 910
PsuI RGATCY 1 cut(s) 511
PsyI GACNNNGTC 1 cut(s) 99
RruI TCGCGA 1 cut(s) 274
RsaI GTAC 3 cut(s) 1024, 1235, 1420
RsaNI GTAC 3 cut(s) 1023, 1234, 1419
SacI GAGCTC 1 cut(s) 253
SalI GTCGAC 1 cut(s) 605
SapI GCTCTTC 1 cut(s) 645
SaqAI TTAA 4 cut(s) 18, 219, 1425, 1429
SatI GCNGC 3 cut(s) 59, 800, 1359
Sau3AI GATC 6 cut(s) 269, 370, 478, 511, 523, 786
Sau96I GGNCC 4 cut(s) 24, 146, 467, 901
ScaI AGTACT 1 cut(s) 1235
SchI GAGTC 4 cut(s) 287, 882, 1069, 1147
ScrFI CCNGG 2 cut(s) 899, 1246
SduI GDGCHC 4 cut(s) 192, 253, 453, 702
SfaNI GCATC 5 cut(s) 84, 106, 150, 568, 1250
SfcI CTRYAG 2 cut(s) 79, 121
SinI GGWCC 3 cut(s) 24, 146, 901
SmlI CTYRAG 2 cut(s) 17, 665
SmoI CTYRAG 2 cut(s) 17, 665
Sse9I AATT 6 cut(s) 483, 782, 841, 942, 1196, 1426
SsiI CCGC 5 cut(s) 58, 623, 1078, 1285, 1358
SspI AATATT 2 cut(s) 1148, 1161
SspMI CTAG 4 cut(s) 173, 383, 488, 1175
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 2 cut(s) 897, 1244
TaaI ACNGT 2 cut(s) 954, 1238
TaiI ACGT 1 cut(s) 450
TaqI TCGA 7 cut(s) 7, 36, 157, 402, 606, 876, 1068
TaqII GACCGA 1 cut(s) 134
TasI AATT 6 cut(s) 483, 782, 841, 942, 1196, 1426
TatI WGTACW 1 cut(s) 1233
TauI GCSGC 2 cut(s) 61, 1361
TfiI GAWTC 7 cut(s) 320, 473, 598, 837, 866, 1070, 1288
Tru1I TTAA 4 cut(s) 18, 219, 1425, 1429
Tru9I TTAA 4 cut(s) 18, 219, 1425, 1429
TscAI CASTG 2 cut(s) 592, 957
TseI GCWGC 1 cut(s) 799
TspDTI ATGAA 9 cut(s) 418, 632, 966, 1070, 1194, 1236, 1241, 1333, 1342
TspGWI ACGGA 3 cut(s) 770, 914, 1323
TspRI CASTG 2 cut(s) 592, 957
Tth111I GACNNNGTC 1 cut(s) 99
Van91I CCANNNNNTGG 1 cut(s) 910
Vha464I CTTAAG 1 cut(s) 17
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 3 cut(s) 24, 146, 901
VspI ATTAAT 1 cut(s) 1425
XapI RAATTY 2 cut(s) 942, 1196
XbaI TCTAGA 1 cut(s) 487
XmiI GTMKAC 2 cut(s) 606, 1171
XspI CTAG 4 cut(s) 173, 383, 488, 1175
ZrmI AGTACT 1 cut(s) 1235
Zsp2I ATGCAT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.