FvH4_4g24680

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000852
Physical Location & Seq
Reverse (-)
26746059 .. 26747793
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24680.t1

Sequence Viewer

Length: 1380 bp
ATGAAGCTTGAAGTTGAACTTGTCTCTAGAGAGCTTATCAGACCTTCTTCTCCAACCCCTGACCACCTCCGCAGATACCAACTCTCCTTTCTCGACCAGATTTCTCCCCCAGTCTATAGTCCTTTGGTTATATTTTTTTCATCAGCCGAGGCTGATAAAAAATTCGACATTGGTGATATAACCAATCGGCTAAAGAAATCCCTCTCTGATGTCTTAGTCCACTTCTACCCACTCGCTGGACGAGCAAATGACAACATGTTCATAGACTGCAATGATGAGGGCACACCCTTCTTGGAAACTCGAGTCAAGTGCCAACTATCTGATGTGATCCAAAATCCAGTTCCTAGTGAACTTAACAAGTTAGTTCCATATCAGCTACACGAAGTTGCTGATCTAGCCCTTGGTGTCCAGCTAAATGTCTTTGAGTGTGGTGGAATTGCAATTGGTGCTTGCATTTCTCACAAGATTGCAGATGCTTTATCATTTTTCATGTTCATCAAAACATGGGCGGGCACTGCTAGAAGAGATCATGGTCACCAGATAGATGATCATACAGTTATGTCTCCACGGTTTGTATCTGCCACACTCTTCCCACCAAGGAACATATCCGGGTTTAATCCAAGTGTTGGGATCACAAAGGAGAACATTGTTACAAAGAGGTTTGTGTTTGATGCTTCAAAGATAGATTTGATTAAATCAAAATATGGTGATAGTGAAGATGAACATGAGCATGAGCATGATAGCCGAGAAAGTCACCAAAGACCGCCATCTCGTGTGGAGGCTCTATCTGCTTTCATATGGAGTCGCTATGTGGCTGCAACTAAAGCAGGGACTGAAGCATCTGAAAGGGTCTATGCCATTGTTCATGCTGTGAACCTTAGGACAAGGATTGATCCACCCCTGCCAGAATGCTCTTTTGGAAATCTCTACCGAATTGCAGTCACGGTTCCACGTCTGGATACAGGGGAGGAGTGCTATGGCCTTGTAAGGCAGGTAAGAGAGCAAGTCAGTCATATCGACAAGGATTATGTGAAGAAATTGCAGGAGGGTAATGAACACTTGAGTTTTATCAAACAAAGCTCTGAGAATTTCACCAAGGGAGAGATGGTCACACTGAGCTTCACTAGTTTGTGCAGATTTCCTCTGTATGAAGCTGATTTTGGATGGGGAAAGCCTACTTGGGTCGGCTCGCCTGCCCTGACTTTCAACAACTTGGTTGTTTTCATGGACACTAAATCTGGGGGTGGAATAGAAGCATATATTAACATGAAGGAAGGAGATTTGGCTAAGATTGAAGCTGACAAAGAGTTCACTGCATTTGTTTCTAGTCGAAGCAATGACGAATGTCTGCCACTGCAGCTCTTGGTTCTCCCCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

460

Amino Acids

51.56

Weight (kDa)

5.74

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 437 1.3e-80 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 982
AccB7I CCANNNNNTGG 2 cut(s) 236, 626
AciI CCGC 3 cut(s) 70, 509, 764
AclWI GGATC 3 cut(s) 322, 638, 887
AcsI RAATTY 2 cut(s) 161, 1087
AcuI CTGAAG 1 cut(s) 855
AfiI CCNNNNNNNGG 2 cut(s) 236, 626
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 5 cut(s) 11, 17, 678, 1207, 1295
AhlI ACTAGT 1 cut(s) 1124
AjiI CACGTC 1 cut(s) 953
AluBI AGCT 9 cut(s) 7, 34, 376, 412, 1080, 1119, 1154, 1298, 1360
AluI AGCT 9 cut(s) 7, 34, 376, 412, 1080, 1119, 1154, 1298, 1360
Alw26I GTCTC 2 cut(s) 28, 567
AlwI GGATC 3 cut(s) 322, 638, 887
AlwNI CAGNNNCTG 1 cut(s) 833
Ama87I CYCGRG 1 cut(s) 300
AoxI GGCC 1 cut(s) 979
ApeKI GCWGC 2 cut(s) 815, 1357
ApoI RAATTY 2 cut(s) 161, 1087
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 5 cut(s) 185, 527, 719, 746, 1084
AvaI CYCGRG 1 cut(s) 300
AxyI CCTNAGG 1 cut(s) 878
BaeGI GKGCMC 2 cut(s) 284, 515
BauI CACGAG 1 cut(s) 771
BbvI GCAGC 2 cut(s) 802, 1369
BccI CCATC 3 cut(s) 775, 1099, 1158
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BciVI GTATCC 1 cut(s) 952
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 2 cut(s) 28, 567
BcuI ACTAGT 1 cut(s) 1124
BfaI CTAG 6 cut(s) 27, 345, 395, 519, 1125, 1326
BfmI CTRYAG 2 cut(s) 115, 1355
BfuAI ACCTGC 1 cut(s) 982
BfuI GTATCC 1 cut(s) 952
BisI GCNGC 2 cut(s) 816, 1358
BlsI GCNGC 2 cut(s) 817, 1359
Bme1390I CCNGG 1 cut(s) 610
BmeT110I CYCGRG 1 cut(s) 300
BmgBI CACGTC 1 cut(s) 953
BmiI GGNNCC 1 cut(s) 948
BmrFI CCNGG 1 cut(s) 610
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 3 cut(s) 463, 661, 848
BmuI ACTGGG 1 cut(s) 104
BoxI GACNNNNGTC 1 cut(s) 1344
BpuEI CTTGAG 1 cut(s) 1081
BpuMI CCSGG 1 cut(s) 610
BsaJI CCNNGG 5 cut(s) 147, 400, 566, 596, 1095
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1092, 1122
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 626
Bse1I ACTGG 2 cut(s) 110, 338
Bse21I CCTNAGG 1 cut(s) 878
Bse3DI GCAATG 2 cut(s) 277, 1342
BseDI CCNNGG 5 cut(s) 147, 400, 566, 596, 1095
BseGI GGATG 1 cut(s) 1169
BseLI CCNNNNNNNGG 2 cut(s) 236, 626
BseMI GCAATG 2 cut(s) 277, 1342
BseMII CTCAG 2 cut(s) 1074, 1106
BseNI ACTGG 2 cut(s) 110, 338
BseRI GAGGAG 1 cut(s) 983
BseSI GKGCMC 2 cut(s) 284, 515
BseXI GCAGC 2 cut(s) 802, 1369
BsgI GTGCAG 1 cut(s) 1153
BshFI GGCC 1 cut(s) 981
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 1 cut(s) 609
BslFI GGGAC 1 cut(s) 844
BslI CCNNNNNNNGG 2 cut(s) 236, 626
BsmAI GTCTC 2 cut(s) 28, 567
BsmFI GGGAC 1 cut(s) 844
BsmI GAATGC 1 cut(s) 914
BsnI GGCC 1 cut(s) 981
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 284, 515
Bsp143I GATC 6 cut(s) 327, 391, 526, 547, 630, 892
BspACI CCGC 3 cut(s) 70, 509, 764
BspANI GGCC 1 cut(s) 981
BspCNI CTCAG 2 cut(s) 1075, 1107
BspLI GGNNCC 1 cut(s) 948
BspMAI CTGCAG 1 cut(s) 1359
BspMI ACCTGC 1 cut(s) 982
BspPI GGATC 3 cut(s) 322, 638, 887
BsrDI GCAATG 2 cut(s) 277, 1342
BsrI ACTGG 2 cut(s) 110, 338
BssECI CCNNGG 5 cut(s) 147, 400, 566, 596, 1095
BssMI GATC 6 cut(s) 327, 391, 526, 547, 630, 892
BssSI CACGAG 1 cut(s) 771
BssT1I CCWWGG 3 cut(s) 400, 596, 1095
Bst2BI CACGAG 1 cut(s) 771
Bst4CI ACNGT 3 cut(s) 556, 570, 946
Bst6I CTCTTC 2 cut(s) 517, 593
BstAPI GCANNNNNTGC 1 cut(s) 446
BstC8I GCNNGC 4 cut(s) 451, 511, 1190, 1194
BstDEI CTNAG 5 cut(s) 214, 878, 1083, 1115, 1287
BstDSI CCRYGG 1 cut(s) 566
BstEII GGTNACC 1 cut(s) 533
BstF5I GGATG 1 cut(s) 1169
BstKTI GATC 6 cut(s) 330, 394, 529, 550, 633, 895
BstMAI GTCTC 2 cut(s) 28, 567
BstMBI GATC 6 cut(s) 327, 391, 526, 547, 630, 892
BstMWI GCNNNNNNNGC 7 cut(s) 242, 395, 446, 515, 788, 824, 1357
BstNSI RCATGY 1 cut(s) 259
BstPAI GACNNNNGTC 1 cut(s) 1344
BstPI GGTNACC 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 608
BstSFI CTRYAG 2 cut(s) 115, 1355
BstSLI GKGCMC 2 cut(s) 284, 515
BstV1I GCAGC 2 cut(s) 802, 1369
Bsu36I CCTNAGG 1 cut(s) 878
BsuI GTATCC 1 cut(s) 952
BsuRI GGCC 1 cut(s) 981
BtgI CCRYGG 1 cut(s) 566
BtrI CACGTC 1 cut(s) 953
BtsCI GGATG 1 cut(s) 1169
BtsI GCAGTG 3 cut(s) 513, 1311, 1352
BtsIMutI CAGTG 4 cut(s) 513, 1112, 1311, 1352
BveI ACCTGC 1 cut(s) 982
Cac8I GCNNGC 4 cut(s) 451, 511, 1190, 1194
CaiI CAGNNNCTG 1 cut(s) 833
DdeI CTNAG 5 cut(s) 214, 878, 1083, 1115, 1287
DpnI GATC 6 cut(s) 329, 393, 528, 549, 632, 894
DpnII GATC 6 cut(s) 327, 391, 526, 547, 630, 892
Eam1104I CTCTTC 2 cut(s) 517, 593
EarI CTCTTC 2 cut(s) 517, 593
Eco130I CCWWGG 3 cut(s) 400, 596, 1095
Eco57I CTGAAG 1 cut(s) 855
Eco81I CCTNAGG 1 cut(s) 878
Eco88I CYCGRG 1 cut(s) 300
Eco91I GGTNACC 1 cut(s) 533
EcoO65I GGTNACC 1 cut(s) 533
EcoT14I CCWWGG 3 cut(s) 400, 596, 1095
ErhI CCWWGG 3 cut(s) 400, 596, 1095
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 1 cut(s) 844
FauI CCCGC 1 cut(s) 502
FauNDI CATATG 1 cut(s) 797
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 816, 1358
FokI GGATG 1 cut(s) 1176
Fsp4HI GCNGC 2 cut(s) 816, 1358
FspBI CTAG 6 cut(s) 27, 345, 395, 519, 1125, 1326
GluI GCNGC 2 cut(s) 816, 1358
HaeIII GGCC 1 cut(s) 981
HapII CCGG 1 cut(s) 609
HindIII AAGCTT 1 cut(s) 5
HinfI GANTC 2 cut(s) 303, 802
HpaII CCGG 1 cut(s) 609
HphI GGTGA 5 cut(s) 185, 527, 719, 746, 1084
Hpy166II GTNNAC 4 cut(s) 220, 350, 874, 1311
Hpy188I TCNGA 5 cut(s) 41, 208, 322, 844, 1084
Hpy188III TCNNGA 3 cut(s) 27, 92, 956
Hpy8I GTNNAC 4 cut(s) 220, 350, 874, 1311
HpyAV CCTTC 4 cut(s) 54, 298, 1264, 1268
HpyCH4III ACNGT 3 cut(s) 556, 570, 946
HpyCH4IV ACGT 1 cut(s) 952
HpyF10VI GCNNNNNNNGC 7 cut(s) 242, 395, 446, 515, 788, 824, 1357
HpyF3I CTNAG 5 cut(s) 214, 878, 1083, 1115, 1287
HpySE526I ACGT 1 cut(s) 952
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 6 cut(s) 327, 391, 526, 547, 630, 892
Lsp1109I GCAGC 2 cut(s) 802, 1369
LweI GCATC 3 cut(s) 463, 661, 848
MaeI CTAG 6 cut(s) 27, 345, 395, 519, 1125, 1326
MaeII ACGT 1 cut(s) 952
MaeIII GTNAC 5 cut(s) 533, 649, 752, 940, 1108
MalI GATC 6 cut(s) 329, 393, 528, 549, 632, 894
MboI GATC 6 cut(s) 327, 391, 526, 547, 630, 892
MboII GAAGA 5 cut(s) 39, 534, 580, 728, 1045
MfeI CAATTG 1 cut(s) 441
MhlI GDGCHC 2 cut(s) 284, 515
MluCI AATT 6 cut(s) 161, 435, 441, 933, 1037, 1087
MlyI GAGTC 2 cut(s) 312, 811
MmeI TCCRAC 1 cut(s) 77
MnlI CCTC 9 cut(s) 77, 142, 212, 271, 651, 772, 961, 1039, 1152
MseI TTAA 4 cut(s) 354, 615, 693, 1263
MslI CAYNNNNRTG 2 cut(s) 729, 735
MspI CCGG 1 cut(s) 609
MspR9I CCNGG 1 cut(s) 610
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 914
MwoI GCNNNNNNNGC 7 cut(s) 242, 395, 446, 515, 788, 824, 1357
NciI CCSGG 1 cut(s) 610
NdeI CATATG 1 cut(s) 797
NdeII GATC 6 cut(s) 327, 391, 526, 547, 630, 892
NlaIV GGNNCC 1 cut(s) 948
NmeAIII GCCGAG 2 cut(s) 172, 770
NmuCI GTSAC 4 cut(s) 533, 752, 940, 1108
NspI RCATGY 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 300
PciI ACATGT 1 cut(s) 255
PctI GAATGC 1 cut(s) 914
PflMI CCANNNNNTGG 2 cut(s) 236, 626
PkrI GCNGC 2 cut(s) 817, 1359
PleI GAGTC 2 cut(s) 311, 810
PpsI GAGTC 2 cut(s) 311, 810
PscI ACATGT 1 cut(s) 255
PshAI GACNNNNGTC 1 cut(s) 1344
PspEI GGTNACC 1 cut(s) 533
PspN4I GGNNCC 1 cut(s) 948
PspXI VCTCGAGB 1 cut(s) 300
PstI CTGCAG 1 cut(s) 1359
PstNI CAGNNNCTG 1 cut(s) 833
RseI CAYNNNNRTG 2 cut(s) 729, 735
SaqAI TTAA 4 cut(s) 354, 615, 693, 1263
SatI GCNGC 2 cut(s) 816, 1358
Sau3AI GATC 6 cut(s) 327, 391, 526, 547, 630, 892
SchI GAGTC 2 cut(s) 312, 811
ScrFI CCNGG 1 cut(s) 610
SduI GDGCHC 2 cut(s) 284, 515
SfaNI GCATC 3 cut(s) 463, 661, 848
SfcI CTRYAG 2 cut(s) 115, 1355
Sfr274I CTCGAG 1 cut(s) 300
SlaI CTCGAG 1 cut(s) 300
SmiMI CAYNNNNRTG 2 cut(s) 729, 735
SmlI CTYRAG 2 cut(s) 300, 1060
SmoI CTYRAG 2 cut(s) 300, 1060
SpeI ACTAGT 1 cut(s) 1124
Sse9I AATT 6 cut(s) 161, 435, 441, 933, 1037, 1087
SsiI CCGC 3 cut(s) 70, 509, 764
SspMI CTAG 6 cut(s) 27, 345, 395, 519, 1125, 1326
StyD4I CCNGG 1 cut(s) 608
StyI CCWWGG 3 cut(s) 400, 596, 1095
TaaI ACNGT 3 cut(s) 556, 570, 946
TaiI ACGT 1 cut(s) 955
TaqI TCGA 5 cut(s) 93, 165, 301, 1017, 1330
TasI AATT 6 cut(s) 161, 435, 441, 933, 1037, 1087
Tru1I TTAA 4 cut(s) 354, 615, 693, 1263
Tru9I TTAA 4 cut(s) 354, 615, 693, 1263
TscAI CASTG 4 cut(s) 520, 1119, 1318, 1359
TseFI GTSAC 4 cut(s) 533, 752, 940, 1108
TseI GCWGC 2 cut(s) 815, 1357
Tsp45I GTSAC 4 cut(s) 533, 752, 940, 1108
TspRI CASTG 4 cut(s) 520, 1119, 1318, 1359
Van91I CCANNNNNTGG 2 cut(s) 236, 626
XapI RAATTY 2 cut(s) 161, 1087
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 1102
XhoI CTCGAG 1 cut(s) 300
XspI CTAG 6 cut(s) 27, 345, 395, 519, 1125, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.