MD13G1109300.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000852
Physical Location & Seq
Forward (+)
7918676 .. 7919983
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1109300.v1.1.491

Sequence Viewer

Length: 1308 bp
ATGAAGGTTGAAGTTGTAGTAATCTCCAAGGAGATTATCAGACCATCTTCTCCAACTCCTGATCACCTTCGCACATACCAACTTTCCTTTCTCGACCAGATATCTCCTCCGGTCTATAATCCTTTGGTCATATTCTATTCCTCATCAGAGCCCGAAACAAAATTCGATATTGTTGAAATATCCAATCAGCTTAAGAAATCCCTATCCAATGCCTTGACTCAATTCTACCCGCTAGCCGGACGTGCCCAAGGCAACTTGTTCATAGACTGCAATGACCAGGGAATACCCTACTTGGAAACTAGAGTTAAGTGCCAACTTTCTGATGTTATTCAAAACCCAATTCCTGTCGAACTTAACAAATTCATTCCATTCCAACTAGATGAAGTTGTTGATATTTCTTTCGGTGTCCAACTCAACATCTTTGAGTGTGGTGGAATAGCTCTCGGTCTGTGCATTTCCCATAAGATTGCGGACGCATTATCGTTTTTCAATTTCATCAAAACTTGGGCAGCCACCGCTAGACAACAAAGTGATCATCTAGTGTGCCCACAATTTGTATCAGCCAAACTCTTCCCACCAAAGAACATATCTGGGTATAGTCCAAGAGTTGGCATCACAAAGGAGAACATTGTAACCAGAATGTTTGTCTTTGAGGCTTCAAAGATAGAGACTCTCAAATCAAAATATGGTGCTGGTGATCACAGCAAGAGCTTAGAAAATCACCAAAAGCCGCCTTCTCGTGTGGAGGCTTTATCCGCTTTCATATGGAGCCGCTTCATGGCTGCCACTCAGGTGAAGTCAGACATTGCACCAAAGAGGATCTACGCCATTGTCCATGCAGTAAACCTTCGTACGAGGATTGATCCGCCATTGCCGGAACACTCATTTGGAAATCTATACAGAATTGCAATGACAATCCCATCTTTGGATATCGGGGAGGAATGCTATGGCCTACTGAGTCAGGTGAGAGACCAAGTGAAGAAAATCGACAAACACTACGTTGAAAACTTGCAGAAGGGCAGTGAGCATTTGGATTTCATCAAACAGACCTCTGATAATGTCTTCAAAGGAGAGATGGTAACACTGAACTTCACCAGCCTGTGTAGGTTTCCACTGTATGAAGCTGATTTTGGATGGGGAAACCCTACTTGGGTTGGCTCGCCAGCCCTAACTTTCAATAATTTGGTTGTTTTCATCGACGCTAAGTCTGGTGGTGGGATTGAAGCATACATTAATATGAAGGAAGAAGACTTGGCTAAAATTGAAGGCGATGGTGAGTTCCTTGCCTTTGTTTCTCCAAATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

436

Amino Acids

48.74

Weight (kDa)

6.03

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 427 1.6e-78 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 608
AciI CCGC 7 cut(s) 230, 470, 516, 731, 756, 772, 866
AclWI GGATC 2 cut(s) 827, 857
AcsI RAATTY 2 cut(s) 161, 359
AfaI GTAC 1 cut(s) 853
AfiI CCNNNNNNNGG 5 cut(s) 236, 608, 778, 925, 1150
AflII CTTAAG 1 cut(s) 191
AhdI GACNNNNNGTC 1 cut(s) 1204
AjiI CACGTC 1 cut(s) 242
AjnI CCWGG 1 cut(s) 276
AjuI GAANNNNNNNTTGG 8 cut(s) 72, 104, 870, 902, 1113, 1132, 1145, 1164
AleI CACNNNNGTG 1 cut(s) 791
AluBI AGCT 4 cut(s) 190, 440, 711, 1124
AluI AGCT 4 cut(s) 190, 440, 711, 1124
Alw26I GTCTC 2 cut(s) 662, 963
AlwI GGATC 2 cut(s) 827, 857
AoxI GGCC 1 cut(s) 949
ApeKI GCWGC 2 cut(s) 509, 782
ApoI RAATTY 2 cut(s) 161, 359
AseI ATTAAT 1 cut(s) 1233
AsuHPI GGTGA 7 cut(s) 56, 707, 713, 805, 976, 1084, 1286
AsuNHI GCTAGC 1 cut(s) 232
BaeGI GKGCMC 2 cut(s) 247, 548
BanII GRGCYC 1 cut(s) 153
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 2 cut(s) 1054, 1254
BbvI GCAGC 2 cut(s) 521, 769
BccI CCATC 5 cut(s) 52, 928, 1069, 1128, 1265
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 3 cut(s) 61, 532, 697
BcoDI GTCTC 2 cut(s) 662, 963
BfaI CTAG 5 cut(s) 233, 300, 377, 519, 539
BfrI CTTAAG 1 cut(s) 191
BisI GCNGC 4 cut(s) 510, 731, 772, 783
BlsI GCNGC 4 cut(s) 511, 732, 773, 784
Bme1390I CCNGG 1 cut(s) 278
BmeRI GACNNNNNGTC 1 cut(s) 1204
BmgBI CACGTC 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 770
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 621
BmtI GCTAGC 1 cut(s) 236
BpiI GAAGAC 2 cut(s) 1054, 1254
BsaI GGTCTC 1 cut(s) 963
BsaJI CCNNGG 3 cut(s) 27, 247, 277
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 1062, 1092
Bsc4I CCNNNNNNNGG 5 cut(s) 236, 608, 778, 925, 1150
Bse3DI GCAATG 4 cut(s) 277, 804, 869, 915
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 27, 247, 277
BseGI GGATG 1 cut(s) 1139
BseLI CCNNNNNNNGG 5 cut(s) 236, 608, 778, 925, 1150
BseMI GCAATG 4 cut(s) 277, 804, 869, 915
BseMII CTCAG 2 cut(s) 803, 947
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 2 cut(s) 247, 548
BseXI GCAGC 2 cut(s) 521, 769
BshFI GGCC 1 cut(s) 951
BsiSI CCGG 3 cut(s) 110, 237, 875
BsiWI CGTACG 1 cut(s) 851
BslI CCNNNNNNNGG 5 cut(s) 236, 608, 778, 925, 1150
BsmAI GTCTC 2 cut(s) 662, 963
BsmI GAATGC 1 cut(s) 947
BsnI GGCC 1 cut(s) 951
Bso31I GGTCTC 1 cut(s) 963
Bsp1286I GDGCHC 3 cut(s) 153, 247, 548
Bsp143I GATC 5 cut(s) 61, 532, 697, 819, 862
BspACI CCGC 7 cut(s) 230, 470, 516, 731, 756, 772, 866
BspANI GGCC 1 cut(s) 951
BspCNI CTCAG 2 cut(s) 802, 948
BspLI GGNNCC 1 cut(s) 770
BspOI GCTAGC 1 cut(s) 236
BspPI GGATC 2 cut(s) 827, 857
BspTI CTTAAG 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 963
BsrDI GCAATG 4 cut(s) 277, 804, 869, 915
BssECI CCNNGG 3 cut(s) 27, 247, 277
BssMI GATC 5 cut(s) 61, 532, 697, 819, 862
BssSI CACGAG 1 cut(s) 738
BssT1I CCWWGG 2 cut(s) 27, 247
Bst2BI CACGAG 1 cut(s) 738
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 1116
Bst6I CTCTTC 1 cut(s) 575
BstAFI CTTAAG 1 cut(s) 191
BstC8I GCNNGC 3 cut(s) 234, 1160, 1164
BstDEI CTNAG 4 cut(s) 712, 789, 956, 1203
BstF5I GGATG 1 cut(s) 1139
BstKTI GATC 5 cut(s) 64, 535, 700, 822, 865
BstMAI GTCTC 2 cut(s) 662, 963
BstMBI GATC 5 cut(s) 61, 532, 697, 819, 862
BstMWI GCNNNNNNNGC 3 cut(s) 242, 515, 755
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstSLI GKGCMC 2 cut(s) 247, 548
BstV1I GCAGC 2 cut(s) 521, 769
BstV2I GAAGAC 2 cut(s) 1054, 1254
BstX2I RGATCY 1 cut(s) 819
BstYI RGATCY 1 cut(s) 819
BsuRI GGCC 1 cut(s) 951
BtgZI GCGATG 1 cut(s) 1284
BtrI CACGTC 1 cut(s) 242
BtsCI GGATG 1 cut(s) 1139
BtsI GCAGTG 1 cut(s) 1027
BtsIMutI CAGTG 3 cut(s) 1027, 1082, 1112
Cac8I GCNNGC 3 cut(s) 234, 1160, 1164
CseI GACGC 2 cut(s) 482, 1208
Csp6I GTAC 1 cut(s) 852
CviAII CATG 2 cut(s) 778, 836
CviQI GTAC 1 cut(s) 852
DdeI CTNAG 4 cut(s) 712, 789, 956, 1203
DpnI GATC 5 cut(s) 63, 534, 699, 821, 864
DpnII GATC 5 cut(s) 61, 532, 697, 819, 862
DriI GACNNNNNGTC 1 cut(s) 1204
Eam1104I CTCTTC 1 cut(s) 575
Eam1105I GACNNNNNGTC 1 cut(s) 1204
EarI CTCTTC 1 cut(s) 575
EciI GGCGGA 1 cut(s) 855
Eco130I CCWWGG 2 cut(s) 27, 247
Eco24I GRGCYC 1 cut(s) 153
Eco31I GGTCTC 1 cut(s) 963
Eco32I GATATC 2 cut(s) 102, 931
EcoRII CCWGG 1 cut(s) 276
EcoRV GATATC 2 cut(s) 102, 931
EcoT14I CCWWGG 2 cut(s) 27, 247
EcoT38I GRGCYC 1 cut(s) 153
ErhI CCWWGG 2 cut(s) 27, 247
FaeI CATG 2 cut(s) 781, 839
FatI CATG 2 cut(s) 777, 835
FauI CCCGC 1 cut(s) 237
FauNDI CATATG 1 cut(s) 764
FbaI TGATCA 3 cut(s) 61, 532, 697
Fnu4HI GCNGC 4 cut(s) 510, 731, 772, 783
FokI GGATG 1 cut(s) 1146
FriOI GRGCYC 1 cut(s) 153
Fsp4HI GCNGC 4 cut(s) 510, 731, 772, 783
FspBI CTAG 5 cut(s) 233, 300, 377, 519, 539
GluI GCNGC 4 cut(s) 510, 731, 772, 783
HaeIII GGCC 1 cut(s) 951
HapII CCGG 3 cut(s) 110, 237, 875
HgaI GACGC 2 cut(s) 482, 1208
Hin1II CATG 2 cut(s) 781, 839
HinfI GANTC 3 cut(s) 217, 670, 958
HpaII CCGG 3 cut(s) 110, 237, 875
HphI GGTGA 7 cut(s) 56, 707, 713, 805, 976, 1084, 1286
Hpy166II GTNNAC 1 cut(s) 844
Hpy188I TCNGA 5 cut(s) 41, 148, 322, 802, 1054
Hpy188III TCNNGA 2 cut(s) 59, 92
Hpy8I GTNNAC 1 cut(s) 844
Hpy99I CGWCG 1 cut(s) 1202
HpyAV CCTTC 6 cut(s) 77, 744, 857, 1009, 1234, 1259
HpyCH4III ACNGT 1 cut(s) 1116
HpyCH4IV ACGT 2 cut(s) 241, 999
HpyCH4V TGCA 6 cut(s) 270, 453, 809, 839, 908, 1012
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 515, 755
HpyF3I CTNAG 4 cut(s) 712, 789, 956, 1203
HpySE526I ACGT 2 cut(s) 241, 999
Hsp92II CATG 2 cut(s) 781, 839
Ksp22I TGATCA 3 cut(s) 61, 532, 697
Kzo9I GATC 5 cut(s) 61, 532, 697, 819, 862
LmnI GCTCC 1 cut(s) 768
Lsp1109I GCAGC 2 cut(s) 521, 769
LweI GCATC 1 cut(s) 621
MaeI CTAG 5 cut(s) 233, 300, 377, 519, 539
MaeII ACGT 2 cut(s) 241, 999
MaeIII GTNAC 2 cut(s) 631, 1078
MalI GATC 5 cut(s) 63, 534, 699, 821, 864
MboI GATC 5 cut(s) 61, 532, 697, 819, 862
MboII GAAGA 6 cut(s) 39, 562, 991, 1054, 1256, 1259
MflI RGATCY 1 cut(s) 819
MhlI GDGCHC 3 cut(s) 153, 247, 548
MluCI AATT 9 cut(s) 161, 221, 339, 359, 490, 551, 903, 1180, 1260
MlyI GAGTC 3 cut(s) 211, 664, 967
MmeI TCCRAC 3 cut(s) 77, 397, 433
MnlI CCTC 8 cut(s) 117, 151, 646, 739, 810, 849, 931, 1060
MseI TTAA 4 cut(s) 192, 306, 354, 1233
MslI CAYNNNNRTG 2 cut(s) 791, 1235
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 3 cut(s) 110, 237, 875
MspR9I CCNGG 1 cut(s) 278
Mva1269I GAATGC 1 cut(s) 947
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 3 cut(s) 242, 515, 755
NdeI CATATG 1 cut(s) 764
NdeII GATC 5 cut(s) 61, 532, 697, 819, 862
NheI GCTAGC 1 cut(s) 232
NlaIII CATG 2 cut(s) 781, 839
NlaIV GGNNCC 1 cut(s) 770
OliI CACNNNNGTG 1 cut(s) 791
PctI GAATGC 1 cut(s) 947
Pfl23II CGTACG 1 cut(s) 851
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 4 cut(s) 511, 732, 773, 784
PleI GAGTC 3 cut(s) 211, 664, 966
PpsI GAGTC 3 cut(s) 211, 664, 966
PshBI ATTAAT 1 cut(s) 1233
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspLI CGTACG 1 cut(s) 851
PspN4I GGNNCC 1 cut(s) 770
PsuI RGATCY 1 cut(s) 819
RsaI GTAC 1 cut(s) 853
RsaNI GTAC 1 cut(s) 852
RseI CAYNNNNRTG 2 cut(s) 791, 1235
SaqAI TTAA 4 cut(s) 192, 306, 354, 1233
SatI GCNGC 4 cut(s) 510, 731, 772, 783
Sau3AI GATC 5 cut(s) 61, 532, 697, 819, 862
SchI GAGTC 3 cut(s) 211, 664, 967
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 3 cut(s) 153, 247, 548
SfaNI GCATC 1 cut(s) 621
SmiMI CAYNNNNRTG 2 cut(s) 791, 1235
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 9 cut(s) 161, 221, 339, 359, 490, 551, 903, 1180, 1260
SsiI CCGC 7 cut(s) 230, 470, 516, 731, 756, 772, 866
SspMI CTAG 5 cut(s) 233, 300, 377, 519, 539
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 2 cut(s) 27, 247
TaaI ACNGT 1 cut(s) 1116
TaiI ACGT 2 cut(s) 244, 1002
TaqI TCGA 5 cut(s) 93, 165, 348, 987, 1197
TaqII GACCGA 1 cut(s) 434
TasI AATT 9 cut(s) 161, 221, 339, 359, 490, 551, 903, 1180, 1260
TauI GCSGC 2 cut(s) 733, 774
Tru1I TTAA 4 cut(s) 192, 306, 354, 1233
Tru9I TTAA 4 cut(s) 192, 306, 354, 1233
TscAI CASTG 3 cut(s) 1027, 1089, 1119
TseI GCWGC 2 cut(s) 509, 782
TspRI CASTG 3 cut(s) 1027, 1089, 1119
Van91I CCANNNNNTGG 1 cut(s) 608
Vha464I CTTAAG 1 cut(s) 191
VspI ATTAAT 1 cut(s) 1233
XapI RAATTY 2 cut(s) 161, 359
XspI CTAG 5 cut(s) 233, 300, 377, 519, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.