Rorug05G0103900

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000852
Physical Location & Seq
Reverse (-)
9143346 .. 9147837
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0103900.1

Sequence Viewer

Length: 1119 bp
ATGGAAGGCGAAGAGGGGCCCGCCGACGCAGCCATCGTCGCCGTCGCCGTCGCCACCGCCGACTCCACTCCACCGTCTCCGCCCAACGACTCCGATGTCTCGCCCGTTGGATCCCCCGATCACGGCGCGTCCTCCGAGATTCTACGGTTGCCGTCCGACGACGACCCCGATCAATCGGCCGGCGGTGCTCTCACCGAGGATCTTAAACAAAAGATTATCAAGCAGGTGGAATATTACTTTAGTGATGAAAATTTGCCTAATGACAAATACATGTTGAGTTTGATTAAAAAGAACAAAGAAGGTTTTGTTCCTATTTCGGTTATTGCGTCCTTCAGAAAAATGAAAAAGCTTATTCGAAACAACTCGTTAATAGTGGCTGCTTTGAAGGACTCTTCCCTCCTTGTTGTGAGTTCGAATGGGAAGAAGGTGAAACGACTTCATCCTCTACCATTACCTGAAACAAGGGATCCAAAGTTCACTGTTTTGGTAGAGAATCTACCAACTGATCATTCAGTGGAGAACCTTAAGAAAATGTTTAGCGAAGCTGGAAATATAAAGGATATATGCATACTTGACCCAAGTGCCATAGAGGCATCTGCAAAAGGCGGCAAGGTTGAGAAGCCAATCAGTAATAAGTTGCATGCTCTTGTAGAGTATGATACCGTGGAGGCTACTGAGAAAGCTGTTGCTATTTTAAACAATGAGGAAGATTGGAGAAATGGCATGCGGGTGAAGCTTCTTAAGCAGATGGGTAAGTACGGGCAGAGTGGACAGAGAAGACAATCCCGCAGGGGCTTTGATTTGGAGAAGAGTAGCATTAGTCGAACGTCTGAAACAGGGATCGAGGAGACTCATAATGCGAACGAACATCACAATGGTACACCTGACGAAGAGGATGGAGGAGACCAAGTGCCCAAGGAGAAAACTGGGCACAGGGGTCGAAGTCGAGGGCAATCTGGTAGGCAGAAGTATCGTGGCCTCAATGGTTTTGGCCACGGAACTACTTCATCTAGTCATCCAATTGAACCTTCAAAGCCACCACCTGGCCCTAGAATGCCTGATGGAACTAGGGGATTCACAATGGGACGGGGCCGGACTCCAGTGCCGAATCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

372

Amino Acids

40.47

Weight (kDa)

7.18

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
La PF05383 72 - 130 2.1e-26 La domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 214
AasI GACNNNNNNGTC 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 1043
AccII CGCG 1 cut(s) 128
AciI CCGC 7 cut(s) 21, 57, 80, 183, 606, 727, 787
AclWI GGATC 6 cut(s) 105, 118, 207, 461, 474, 848
AcoI YGGCCR 2 cut(s) 177, 991
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 316
AfaI GTAC 2 cut(s) 758, 880
AfiI CCNNNNNNNGG 2 cut(s) 122, 1043
AflII CTTAAG 2 cut(s) 524, 740
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 3 cut(s) 385, 1025, 1032
AjnI CCWGG 1 cut(s) 1042
AjuI GAANNNNNNNTTGG 4 cut(s) 493, 525, 1012, 1044
AloI GAACNNNNNNTCC 2 cut(s) 458, 490
AluBI AGCT 4 cut(s) 349, 545, 683, 736
AluI AGCT 4 cut(s) 349, 545, 683, 736
Alw21I GWGCWC 1 cut(s) 190
Alw26I GTCTC 4 cut(s) 81, 103, 842, 897
AlwI GGATC 6 cut(s) 105, 118, 207, 461, 474, 848
AoxI GGCC 6 cut(s) 17, 177, 976, 991, 1045, 1090
ApaI GGGCCC 1 cut(s) 21
ApeKI GCWGC 2 cut(s) 29, 377
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 1 cut(s) 1003
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 4 cut(s) 17, 18, 1046, 1090
AsuHPI GGTGA 3 cut(s) 184, 439, 742
AsuII TTCGAA 2 cut(s) 355, 413
BaeGI GKGCMC 3 cut(s) 21, 915, 933
BalI TGGCCA 1 cut(s) 993
BamHI GGATCC 2 cut(s) 110, 466
BanII GRGCYC 1 cut(s) 21
BbsI GAAGAC 1 cut(s) 784
Bbv12I GWGCWC 1 cut(s) 190
BbvI GCAGC 2 cut(s) 41, 364
BccI CCATC 4 cut(s) 41, 742, 890, 1055
BceAI ACGGC 4 cut(s) 26, 32, 136, 139
BcgI CGANNNNNNTGC 4 cut(s) 920, 953, 954, 987
BciT130I CCWGG 1 cut(s) 1044
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 4 cut(s) 81, 103, 842, 897
BfaI CTAG 3 cut(s) 1011, 1050, 1068
BfrI CTTAAG 2 cut(s) 524, 740
BfuAI ACCTGC 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 590
BisI GCNGC 3 cut(s) 30, 378, 607
BlsI GCNGC 3 cut(s) 31, 379, 608
Bme1390I CCNGG 1 cut(s) 1044
BmgT120I GGNCC 4 cut(s) 17, 18, 1046, 1090
BmiI GGNNCC 5 cut(s) 18, 19, 112, 468, 1091
BmrFI CCNGG 1 cut(s) 1044
BmrI ACTGGG 1 cut(s) 936
BmsI GCATC 1 cut(s) 602
BmuI ACTGGG 1 cut(s) 936
BpiI GAAGAC 1 cut(s) 784
BpmI CTGGAG 1 cut(s) 1083
Bpu14I TTCGAA 2 cut(s) 355, 413
BsaI GGTCTC 1 cut(s) 897
BsaJI CCNNGG 4 cut(s) 195, 663, 915, 994
BsaXI ACNNNNNCTCC 2 cut(s) 894, 924
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 1043
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 2 cut(s) 931, 1100
BseBI CCWGG 1 cut(s) 1044
BseDI CCNNGG 4 cut(s) 195, 663, 915, 994
BseGI GGATG 3 cut(s) 439, 901, 1015
BseLI CCNNNNNNNGG 2 cut(s) 122, 1043
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 2 cut(s) 931, 1100
BseRI GAGGAG 2 cut(s) 860, 915
BseSI GKGCMC 3 cut(s) 21, 915, 933
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 2 cut(s) 41, 364
Bsh1236I CGCG 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 6 cut(s) 19, 179, 978, 993, 1047, 1092
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 190
BsiSI CCGG 2 cut(s) 180, 1093
BslFI GGGAC 1 cut(s) 1098
BslI CCNNNNNNNGG 2 cut(s) 122, 1043
BsmAI GTCTC 4 cut(s) 81, 103, 842, 897
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 1 cut(s) 1098
BsmI GAATGC 1 cut(s) 1059
BsnI GGCC 6 cut(s) 19, 179, 978, 993, 1047, 1092
Bso31I GGTCTC 1 cut(s) 897
Bsp119I TTCGAA 2 cut(s) 355, 413
Bsp120I GGGCCC 1 cut(s) 17
Bsp1286I GDGCHC 4 cut(s) 21, 190, 915, 933
Bsp143I GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
BspACI CCGC 7 cut(s) 21, 57, 80, 183, 606, 727, 787
BspANI GGCC 6 cut(s) 19, 179, 978, 993, 1047, 1092
BspCNI CTCAG 1 cut(s) 667
BspFNI CGCG 1 cut(s) 128
BspLI GGNNCC 5 cut(s) 18, 19, 112, 468, 1091
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 6 cut(s) 105, 118, 207, 461, 474, 848
BspT104I TTCGAA 2 cut(s) 355, 413
BspTI CTTAAG 2 cut(s) 524, 740
BspTNI GGTCTC 1 cut(s) 897
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 2 cut(s) 931, 1100
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 4 cut(s) 195, 663, 915, 994
BssMI GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
BssT1I CCWWGG 1 cut(s) 915
Bst2UI CCWGG 1 cut(s) 1044
Bst4CI ACNGT 4 cut(s) 75, 147, 481, 664
Bst6I CTCTTC 4 cut(s) 6, 397, 803, 885
BstAFI CTTAAG 2 cut(s) 524, 740
BstBI TTCGAA 2 cut(s) 355, 413
BstC8I GCNNGC 4 cut(s) 21, 181, 642, 725
BstDEI CTNAG 1 cut(s) 675
BstDSI CCRYGG 2 cut(s) 663, 994
BstF5I GGATG 3 cut(s) 439, 901, 1015
BstFNI CGCG 1 cut(s) 128
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 7 cut(s) 113, 121, 172, 202, 469, 508, 843
BstMAI GTCTC 4 cut(s) 81, 103, 842, 897
BstMBI GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 6 cut(s) 29, 38, 185, 590, 733, 742
BstNI CCWGG 1 cut(s) 1044
BstNSI RCATGY 3 cut(s) 274, 644, 727
BstSCI CCNGG 1 cut(s) 1042
BstSLI GKGCMC 3 cut(s) 21, 915, 933
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 2 cut(s) 41, 364
BstV2I GAAGAC 1 cut(s) 784
BstX2I RGATCY 3 cut(s) 110, 199, 466
BstYI RGATCY 3 cut(s) 110, 199, 466
BstZI CGGCCG 1 cut(s) 177
BsuRI GGCC 6 cut(s) 19, 179, 978, 993, 1047, 1092
BtgI CCRYGG 2 cut(s) 663, 994
BtsCI GGATG 3 cut(s) 439, 901, 1015
BtsIMutI CAGTG 3 cut(s) 477, 519, 1107
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 21, 181, 642, 725
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 4 cut(s) 17, 18, 1046, 1090
CseI GACGC 3 cut(s) 35, 117, 315
Csp6I GTAC 2 cut(s) 757, 879
CviAII CATG 3 cut(s) 271, 641, 724
CviQI GTAC 2 cut(s) 757, 879
DdeI CTNAG 1 cut(s) 675
DpnI GATC 7 cut(s) 112, 120, 171, 201, 468, 507, 842
DpnII GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
DraI TTTAAA 1 cut(s) 696
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 2 cut(s) 177, 991
EagI CGGCCG 1 cut(s) 177
Eam1104I CTCTTC 4 cut(s) 6, 397, 803, 885
EarI CTCTTC 4 cut(s) 6, 397, 803, 885
EciI GGCGGA 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 915
Eco24I GRGCYC 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 897
Eco52I CGGCCG 1 cut(s) 177
Eco57I CTGAAG 1 cut(s) 316
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 1042
EcoT14I CCWWGG 1 cut(s) 915
EcoT22I ATGCAT 1 cut(s) 569
EcoT38I GRGCYC 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 915
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 3 cut(s) 274, 644, 727
FaqI GGGAC 1 cut(s) 1098
FatI CATG 3 cut(s) 270, 640, 723
FauI CCCGC 3 cut(s) 28, 720, 794
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 30, 378, 607
FokI GGATG 3 cut(s) 426, 908, 1002
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 3 cut(s) 30, 378, 607
FspBI CTAG 3 cut(s) 1011, 1050, 1068
GlaI GCGC 1 cut(s) 127
GluI GCNGC 3 cut(s) 30, 378, 607
GsuI CTGGAG 1 cut(s) 1083
HaeIII GGCC 6 cut(s) 19, 179, 978, 993, 1047, 1092
HapII CCGG 2 cut(s) 180, 1093
HgaI GACGC 3 cut(s) 35, 117, 315
HhaI GCGC 1 cut(s) 128
Hin1II CATG 3 cut(s) 274, 644, 727
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HindIII AAGCTT 2 cut(s) 347, 734
HinfI GANTC 9 cut(s) 62, 89, 139, 389, 493, 850, 1074, 1096, 1108
HpaII CCGG 2 cut(s) 180, 1093
HphI GGTGA 3 cut(s) 184, 439, 742
Hpy166II GTNNAC 3 cut(s) 477, 770, 881
Hpy188I TCNGA 6 cut(s) 94, 136, 157, 335, 832, 1113
Hpy8I GTNNAC 3 cut(s) 477, 770, 881
Hpy99I CGWCG 6 cut(s) 29, 41, 47, 53, 161, 164
HpyAV CCTTC 5 cut(s) 293, 340, 379, 418, 1038
HpyCH4III ACNGT 4 cut(s) 75, 147, 481, 664
HpyCH4IV ACGT 1 cut(s) 827
HpyCH4V TGCA 3 cut(s) 567, 599, 640
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 38, 185, 590, 733, 742
HpyF3I CTNAG 1 cut(s) 675
HpySE526I ACGT 1 cut(s) 827
Hsp92II CATG 3 cut(s) 274, 644, 727
HspAI GCGC 1 cut(s) 126
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
Lsp1109I GCAGC 2 cut(s) 41, 364
LweI GCATC 1 cut(s) 602
MaeI CTAG 3 cut(s) 1011, 1050, 1068
MaeII ACGT 1 cut(s) 827
MalI GATC 7 cut(s) 112, 120, 171, 201, 468, 507, 842
MboI GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
MboII GAAGA 7 cut(s) 23, 384, 433, 719, 789, 820, 902
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 3 cut(s) 110, 199, 466
MhlI GDGCHC 4 cut(s) 21, 190, 915, 933
MlsI TGGCCA 1 cut(s) 993
MluCI AATT 2 cut(s) 250, 1020
MluNI TGGCCA 1 cut(s) 993
MlyI GAGTC 5 cut(s) 56, 83, 383, 844, 1090
MmeI TCCRAC 2 cut(s) 88, 180
Mox20I TGGCCA 1 cut(s) 993
Mph1103I ATGCAT 1 cut(s) 569
MroNI GCCGGC 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 1003
MscI TGGCCA 1 cut(s) 993
MseI TTAA 7 cut(s) 204, 285, 368, 525, 695, 741, 1117
MslI CAYNNNNRTG 2 cut(s) 728, 873
Msp20I TGGCCA 1 cut(s) 993
MspCI CTTAAG 2 cut(s) 524, 740
MspI CCGG 2 cut(s) 180, 1093
MspR9I CCNGG 1 cut(s) 1044
MunI CAATTG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 1059
MvaI CCWGG 1 cut(s) 1044
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 6 cut(s) 29, 38, 185, 590, 733, 742
NaeI GCCGGC 1 cut(s) 181
NdeII GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 3 cut(s) 274, 644, 727
NlaIV GGNNCC 5 cut(s) 18, 19, 112, 468, 1091
NsiI ATGCAT 1 cut(s) 569
NspI RCATGY 3 cut(s) 274, 644, 727
NspV TTCGAA 2 cut(s) 355, 413
PaeI GCATGC 2 cut(s) 644, 727
PaqCI CACCTGC 1 cut(s) 214
PciI ACATGT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 5 cut(s) 33, 45, 57, 165, 323
PctI GAATGC 1 cut(s) 1059
PdiI GCCGGC 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 1003
PfeI GAWTC 4 cut(s) 139, 493, 1074, 1108
PflMI CCANNNNNTGG 1 cut(s) 1043
PkrI GCNGC 3 cut(s) 31, 379, 608
PleI GAGTC 5 cut(s) 56, 83, 383, 844, 1090
PpsI GAGTC 5 cut(s) 56, 83, 383, 844, 1090
PscI ACATGT 1 cut(s) 270
Psp6I CCWGG 1 cut(s) 1042
PspGI CCWGG 1 cut(s) 1042
PspN4I GGNNCC 5 cut(s) 18, 19, 112, 468, 1091
PspOMI GGGCCC 1 cut(s) 17
PspPI GGNCC 4 cut(s) 17, 18, 1046, 1090
PsuI RGATCY 3 cut(s) 110, 199, 466
RsaI GTAC 2 cut(s) 758, 880
RsaNI GTAC 2 cut(s) 757, 879
RseI CAYNNNNRTG 2 cut(s) 728, 873
SaqAI TTAA 7 cut(s) 204, 285, 368, 525, 695, 741, 1117
SatI GCNGC 3 cut(s) 30, 378, 607
Sau3AI GATC 7 cut(s) 110, 118, 169, 199, 466, 505, 840
Sau96I GGNCC 4 cut(s) 17, 18, 1046, 1090
SchI GAGTC 5 cut(s) 56, 83, 383, 844, 1090
ScrFI CCNGG 1 cut(s) 1044
SduI GDGCHC 4 cut(s) 21, 190, 915, 933
SfaNI GCATC 1 cut(s) 602
SfuI TTCGAA 2 cut(s) 355, 413
SmiMI CAYNNNNRTG 2 cut(s) 728, 873
SmlI CTYRAG 2 cut(s) 524, 740
SmoI CTYRAG 2 cut(s) 524, 740
SphI GCATGC 2 cut(s) 644, 727
Sse9I AATT 2 cut(s) 250, 1020
SsiI CCGC 7 cut(s) 21, 57, 80, 183, 606, 727, 787
SspI AATATT 1 cut(s) 233
SspMI CTAG 3 cut(s) 1011, 1050, 1068
StyD4I CCNGG 1 cut(s) 1042
StyI CCWWGG 1 cut(s) 915
TaaI ACNGT 4 cut(s) 75, 147, 481, 664
TaiI ACGT 1 cut(s) 830
TaqI TCGA 6 cut(s) 355, 413, 823, 843, 940, 946
TasI AATT 2 cut(s) 250, 1020
TauI GCSGC 1 cut(s) 609
TfiI GAWTC 4 cut(s) 139, 493, 1074, 1108
Tru1I TTAA 7 cut(s) 204, 285, 368, 525, 695, 741, 1117
Tru9I TTAA 7 cut(s) 204, 285, 368, 525, 695, 741, 1117
TscAI CASTG 3 cut(s) 484, 519, 1107
TseI GCWGC 2 cut(s) 29, 377
TspDTI ATGAA 4 cut(s) 261, 356, 428, 996
TspGWI ACGGA 1 cut(s) 1011
TspRI CASTG 3 cut(s) 484, 519, 1107
Van91I CCANNNNNTGG 1 cut(s) 1043
Vha464I CTTAAG 2 cut(s) 524, 740
XapI RAATTY 1 cut(s) 250
XceI RCATGY 3 cut(s) 274, 644, 727
XmnI GAANNNNTTC 1 cut(s) 1003
XspI CTAG 3 cut(s) 1011, 1050, 1068
Zsp2I ATGCAT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.