FvH4_4g24790

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000852
Physical Location & Seq
Reverse (-)
26799593 .. 26800879
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24790.t1

Sequence Viewer

Length: 1287 bp
ATGAAGGTTGAAGTGATTGAGAGGGATTGCATCAAGCCTTCTTCTCCAACCCCAAACCACCTTCGCAAACACGAGCTTTCATTTCTCGATCAAGTTGCCCCTCCCATTTTCATGCCCATGCTTCTTTTCTACCCTGCTACTAGTTATGGTAACGACGAAACACCTTTGTCCGCTAGCCGGGTTAAGCAGTCCCTATCGGAGACCTTAACACAGTTCTATCCGCTAGCCGGAAGGATTATCGACAACGCCTTCGTCAACTGCAACGACGAAGGTACTCCGTTCGTCGAAGCACGAGTCAAGTGCCTCCTTTCAGACGTTGTATCCCAACCGGACCCGAACCAACTCAACAAGCTACTACCATGTGATTTGGAATGTGTGGTCGACTTTGTTCTTGCAGTCCAAGTCAACATCTTTGATTGTGGCGGCATGGCTATCGGAGTTTGCATTTCGCACAAAGTCGCTGACGCCTTGTCACTAGTCATGTTTCTCAACTCTTGGGCGTCCATTTCCCGAGGAGATAACTGTAACTACAGAGCTCCCATTTTTGACTTGGCTACGTTCTTCCCGCCAAGATCCATATCGGGGTTCAAACCAGGAGTTGGAATAGCAAGAGACAAAATCGTTACAAAACGATTCGTTTTTAGCGCCTCCAACGTCGCTTCTCTCCGAACAAAATACACGACGTCTGAAACTGAGAGACGCCCGACTCGTATCGAGGCCCTGTCAGCTTTTGTATGGAGCCGTTTTTTGGCTTCCACGCAAGGAAAGCCCAACTCGGACATAGTTTACAAAGTTCTCCACGCTGTAAACCTACGTACCAGAATGGAACCGGCTCTTCCGGAGCACCATTTCGGAAATGTGAGCAGGTTCGCGACGGCGACGACGTCGTTTGAGGAGGGCGAGGAGGAGTGTTATGGGTTTGTGGGACAAATGAGAGATGCCATAAAGAGCGTCGACGGGGAGTACATGAGGAAGATTTTAGAAGGCACGGCGCATGTTGATTATTTGAAGCAGAGAGCGGAGGAGTTTCTGAAAGGAGAAGTGGTTTCGTTTAGCTTCACCAGTCTGTGTAGGTTTCCGTTGTATGAGACTGATTTTGGGTGGGGGAAGCCGATATGGGTCGGGTCTGCTAGCCTCACGTTCAAGAATTTGGTTTGTTTCATGGATTCTGGGTCTAGTGGTGGGATCGAAGCGTGGGTTAATTTGAAGGAAGAAGACATGGCTAAGTTTCAAGAGGATGAGCAGTTGCGCGCACTCAGTTATCCAGCCAGAGATGCTAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

429

Amino Acids

47.84

Weight (kDa)

5.77

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 415 2e-90 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 686, 887
Acc36I ACCTGC 1 cut(s) 855
AccB7I CCANNNNNTGG 1 cut(s) 599
AccBSI CCGCTC 1 cut(s) 1019
AccI GTMKAC 2 cut(s) 381, 954
AccII CGCG 2 cut(s) 872, 1251
AccIII TCCGGA 1 cut(s) 838
AciI CCGC 5 cut(s) 171, 221, 423, 566, 1019
AclWI GGATC 2 cut(s) 567, 1193
AcsI RAATTY 1 cut(s) 1147
AcyI GRCGYC 5 cut(s) 465, 500, 683, 700, 884
AfaI GTAC 3 cut(s) 274, 817, 965
AfiI CCNNNNNNNGG 5 cut(s) 177, 227, 582, 599, 748
AgsI TTSAA 6 cut(s) 11, 589, 1009, 1144, 1207, 1232
AhdI GACNNNNNGTC 1 cut(s) 469
AhlI ACTAGT 2 cut(s) 140, 475
AjnI CCWGG 1 cut(s) 592
AluBI AGCT 5 cut(s) 76, 352, 536, 728, 1056
AluI AGCT 5 cut(s) 76, 352, 536, 728, 1056
Alw21I GWGCWC 2 cut(s) 538, 846
Alw26I GTCTC 4 cut(s) 194, 606, 691, 1082
AlwI GGATC 2 cut(s) 567, 1193
Ama87I CYCGRG 1 cut(s) 510
Aor13HI TCCGGA 1 cut(s) 838
AoxI GGCC 1 cut(s) 717
ApoI RAATTY 1 cut(s) 1147
ArsI GACNNNNNNTTYG 4 cut(s) 233, 265, 279, 311
AspLEI GCGC 4 cut(s) 647, 994, 1251, 1253
AspS9I GGNCC 2 cut(s) 331, 718
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 1051
AsuNHI GCTAGC 3 cut(s) 173, 223, 1130
AvaI CYCGRG 1 cut(s) 510
AvaII GGWCC 1 cut(s) 331
BaeI ACNNNNGTAYC 2 cut(s) 799, 832
BanII GRGCYC 1 cut(s) 538
BauI CACGAG 2 cut(s) 71, 291
BbsI GAAGAC 1 cut(s) 1221
Bbv12I GWGCWC 2 cut(s) 538, 846
BceAI ACGGC 3 cut(s) 726, 891, 1005
BcgI CGANNNNNNTGC 6 cut(s) 77, 111, 282, 316, 415, 449
BciT130I CCWGG 1 cut(s) 594
BciVI GTATCC 1 cut(s) 331
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 4 cut(s) 194, 606, 691, 1082
BcuI ACTAGT 2 cut(s) 140, 475
BfaI CTAG 6 cut(s) 141, 174, 224, 476, 1131, 1176
BfmI CTRYAG 1 cut(s) 529
BfoI RGCGCY 1 cut(s) 648
BfuAI ACCTGC 1 cut(s) 855
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 1 cut(s) 424
BlsI GCNGC 1 cut(s) 425
Bme1390I CCNGG 2 cut(s) 179, 594
Bme18I GGWCC 1 cut(s) 331
BmeRI GACNNNNNGTC 1 cut(s) 469
BmeT110I CYCGRG 1 cut(s) 510
BmgT120I GGNCC 2 cut(s) 331, 718
BmiI GGNNCC 3 cut(s) 333, 740, 828
BmrFI CCNGG 2 cut(s) 179, 594
BmsI GCATC 3 cut(s) 39, 928, 1264
BmtI GCTAGC 3 cut(s) 177, 227, 1134
BpiI GAAGAC 1 cut(s) 1221
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 815
BsaBI GATNNNNATC 1 cut(s) 577
BsaHI GRCGYC 5 cut(s) 465, 500, 683, 700, 884
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 1 cut(s) 511
BsaWI WCCGGW 2 cut(s) 328, 838
BsaXI ACNNNNNCTCC 2 cut(s) 1029, 1059
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 227, 582, 599, 748
Bse118I RCCGGY 1 cut(s) 829
Bse1I ACTGG 1 cut(s) 1062
Bse8I GATNNNNATC 1 cut(s) 577
BseAI TCCGGA 1 cut(s) 838
BseBI CCWGG 1 cut(s) 594
BseDI CCNNGG 1 cut(s) 511
BseGI GGATG 1 cut(s) 1243
BseJI GATNNNNATC 1 cut(s) 577
BseLI CCNNNNNNNGG 5 cut(s) 177, 227, 582, 599, 748
BseMII CTCAG 2 cut(s) 684, 1270
BseNI ACTGG 1 cut(s) 1062
BsePI GCGCGC 1 cut(s) 1249
BseRI GAGGAG 5 cut(s) 528, 908, 917, 920, 1037
Bsh1236I CGCG 2 cut(s) 872, 1251
BshFI GGCC 1 cut(s) 719
BsiHKAI GWGCWC 2 cut(s) 538, 846
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 5 cut(s) 178, 228, 329, 830, 839
BslFI GGGAC 2 cut(s) 175, 939
BslI CCNNNNNNNGG 5 cut(s) 177, 227, 582, 599, 748
BsmAI GTCTC 4 cut(s) 194, 606, 691, 1082
BsmBI CGTCTC 1 cut(s) 691
BsmFI GGGAC 2 cut(s) 175, 939
BsnI GGCC 1 cut(s) 719
Bso31I GGTCTC 1 cut(s) 194
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 2 cut(s) 538, 846
Bsp13I TCCGGA 1 cut(s) 838
Bsp143I GATC 3 cut(s) 88, 572, 1185
Bsp68I TCGCGA 1 cut(s) 872
BspACI CCGC 5 cut(s) 171, 221, 423, 566, 1019
BspANI GGCC 1 cut(s) 719
BspCNI CTCAG 2 cut(s) 685, 1269
BspEI TCCGGA 1 cut(s) 838
BspFNI CGCG 2 cut(s) 872, 1251
BspLI GGNNCC 3 cut(s) 333, 740, 828
BspMI ACCTGC 1 cut(s) 855
BspOI GCTAGC 3 cut(s) 177, 227, 1134
BspPI GGATC 2 cut(s) 567, 1193
BspQI GCTCTTC 1 cut(s) 840
BspTNI GGTCTC 1 cut(s) 194
BsrBI CCGCTC 1 cut(s) 1019
BsrFI RCCGGY 1 cut(s) 829
BsrI ACTGG 1 cut(s) 1062
BssAI RCCGGY 1 cut(s) 829
BssECI CCNNGG 1 cut(s) 511
BssHII GCGCGC 1 cut(s) 1249
BssMI GATC 3 cut(s) 88, 572, 1185
BssNI GRCGYC 5 cut(s) 465, 500, 683, 700, 884
BssSI CACGAG 2 cut(s) 71, 291
Bst2BI CACGAG 2 cut(s) 71, 291
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 2 cut(s) 213, 524
Bst6I CTCTTC 1 cut(s) 840
BstACI GRCGYC 5 cut(s) 465, 500, 683, 700, 884
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 4 cut(s) 175, 225, 1132, 1251
BstDEI CTNAG 3 cut(s) 693, 1224, 1256
BstF5I GGATG 1 cut(s) 1243
BstFNI CGCG 2 cut(s) 872, 1251
BstH2I RGCGCY 1 cut(s) 648
BstHHI GCGC 4 cut(s) 647, 994, 1251, 1253
BstKTI GATC 3 cut(s) 91, 575, 1188
BstMAI GTCTC 4 cut(s) 194, 606, 691, 1082
BstMBI GATC 3 cut(s) 88, 572, 1185
BstMWI GCNNNNNNNGC 3 cut(s) 725, 766, 1274
BstNI CCWGG 1 cut(s) 594
BstNSI RCATGY 1 cut(s) 998
BstSCI CCNGG 2 cut(s) 177, 592
BstSFI CTRYAG 1 cut(s) 529
BstSNI TACGTA 1 cut(s) 815
BstUI CGCG 2 cut(s) 872, 1251
BstV2I GAAGAC 1 cut(s) 1221
BstX2I RGATCY 1 cut(s) 572
BstYI RGATCY 1 cut(s) 572
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 1 cut(s) 719
BtsCI GGATG 1 cut(s) 1243
BtuMI TCGCGA 1 cut(s) 872
BveI ACCTGC 1 cut(s) 855
Cac8I GCNNGC 4 cut(s) 175, 225, 1132, 1251
CfoI GCGC 4 cut(s) 647, 994, 1251, 1253
Cfr10I RCCGGY 1 cut(s) 829
Cfr13I GGNCC 2 cut(s) 331, 718
CseI GACGC 4 cut(s) 473, 489, 708, 940
Csp6I GTAC 3 cut(s) 273, 816, 964
CviAII CATG 9 cut(s) 112, 118, 360, 427, 481, 967, 995, 1162, 1219
CviQI GTAC 3 cut(s) 273, 816, 964
DdeI CTNAG 3 cut(s) 693, 1224, 1256
DpnI GATC 3 cut(s) 90, 574, 1187
DpnII GATC 3 cut(s) 88, 572, 1185
DriI GACNNNNNGTC 1 cut(s) 469
Eam1104I CTCTTC 1 cut(s) 840
Eam1105I GACNNNNNGTC 1 cut(s) 469
EarI CTCTTC 1 cut(s) 840
Ecl136II GAGCTC 1 cut(s) 536
Eco105I TACGTA 1 cut(s) 815
Eco24I GRGCYC 1 cut(s) 538
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 331
Eco53kI GAGCTC 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 536
EcoO109I RGGNCCY 1 cut(s) 718
EcoRII CCWGG 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 538
Esp3I CGTCTC 1 cut(s) 691
FaeI CATG 9 cut(s) 115, 121, 363, 430, 484, 970, 998, 1165, 1222
FaqI GGGAC 2 cut(s) 175, 939
FatI CATG 9 cut(s) 111, 117, 359, 426, 480, 966, 994, 1161, 1218
FauI CCCGC 1 cut(s) 573
FblI GTMKAC 2 cut(s) 381, 954
Fnu4HI GCNGC 1 cut(s) 424
FokI GGATG 1 cut(s) 1250
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 1 cut(s) 424
FspBI CTAG 6 cut(s) 141, 174, 224, 476, 1131, 1176
GlaI GCGC 4 cut(s) 646, 993, 1250, 1252
GluI GCNGC 1 cut(s) 424
HaeII RGCGCY 1 cut(s) 648
HaeIII GGCC 1 cut(s) 719
HapII CCGG 5 cut(s) 178, 228, 329, 830, 839
HgaI GACGC 4 cut(s) 473, 489, 708, 940
HhaI GCGC 4 cut(s) 647, 994, 1251, 1253
Hin1I GRCGYC 5 cut(s) 465, 500, 683, 700, 884
Hin1II CATG 9 cut(s) 115, 121, 363, 430, 484, 970, 998, 1165, 1222
Hin6I GCGC 4 cut(s) 645, 992, 1249, 1251
HinP1I GCGC 4 cut(s) 645, 992, 1249, 1251
HincII GTYRAC 4 cut(s) 256, 382, 406, 955
HindII GTYRAC 4 cut(s) 256, 382, 406, 955
HinfI GANTC 4 cut(s) 294, 633, 706, 1166
HpaII CCGG 5 cut(s) 178, 228, 329, 830, 839
HphI GGTGA 1 cut(s) 1051
Hpy166II GTNNAC 6 cut(s) 256, 382, 406, 787, 808, 955
Hpy188I TCNGA 8 cut(s) 199, 313, 437, 668, 688, 778, 854, 1032
Hpy188III TCNNGA 6 cut(s) 86, 510, 839, 871, 1144, 1232
Hpy8I GTNNAC 6 cut(s) 256, 382, 406, 787, 808, 955
HpyAV CCTTC 7 cut(s) 48, 71, 225, 259, 263, 977, 1201
HpyCH4III ACNGT 2 cut(s) 213, 524
HpyCH4IV ACGT 7 cut(s) 315, 557, 654, 683, 814, 884, 1139
HpyCH4V TGCA 4 cut(s) 30, 261, 395, 444
HpyF10VI GCNNNNNNNGC 3 cut(s) 725, 766, 1274
HpyF3I CTNAG 3 cut(s) 693, 1224, 1256
HpySE526I ACGT 7 cut(s) 315, 557, 654, 683, 814, 884, 1139
Hsp92I GRCGYC 5 cut(s) 465, 500, 683, 700, 884
Hsp92II CATG 9 cut(s) 115, 121, 363, 430, 484, 970, 998, 1165, 1222
HspAI GCGC 4 cut(s) 645, 992, 1249, 1251
Kpn2I TCCGGA 1 cut(s) 838
Kzo9I GATC 3 cut(s) 88, 572, 1185
LguI GCTCTTC 1 cut(s) 840
LmnI GCTCC 3 cut(s) 541, 738, 841
LweI GCATC 3 cut(s) 39, 928, 1264
MaeI CTAG 6 cut(s) 141, 174, 224, 476, 1131, 1176
MaeII ACGT 7 cut(s) 315, 557, 654, 683, 814, 884, 1139
MaeIII GTNAC 4 cut(s) 149, 471, 524, 622
MalI GATC 3 cut(s) 90, 574, 1187
MbiI CCGCTC 1 cut(s) 1019
MboI GATC 3 cut(s) 88, 572, 1185
MboII GAAGA 6 cut(s) 33, 553, 827, 985, 1223, 1226
MflI RGATCY 1 cut(s) 572
MhlI GDGCHC 2 cut(s) 538, 846
MluCI AATT 2 cut(s) 1147, 1201
MlyI GAGTC 2 cut(s) 303, 700
MmeI TCCRAC 3 cut(s) 71, 580, 675
MroI TCCGGA 1 cut(s) 838
MseI TTAA 3 cut(s) 183, 206, 1200
MslI CAYNNNNRTG 2 cut(s) 110, 116
MspI CCGG 5 cut(s) 178, 228, 329, 830, 839
MspR9I CCNGG 2 cut(s) 179, 594
MvaI CCWGG 1 cut(s) 594
MvnI CGCG 2 cut(s) 872, 1251
MwoI GCNNNNNNNGC 3 cut(s) 725, 766, 1274
NciI CCSGG 1 cut(s) 179
NdeII GATC 3 cut(s) 88, 572, 1185
NheI GCTAGC 3 cut(s) 173, 223, 1130
NlaIII CATG 9 cut(s) 115, 121, 363, 430, 484, 970, 998, 1165, 1222
NlaIV GGNNCC 3 cut(s) 333, 740, 828
NmuCI GTSAC 1 cut(s) 471
NruI TCGCGA 1 cut(s) 872
NspI RCATGY 1 cut(s) 998
PauI GCGCGC 1 cut(s) 1249
PciSI GCTCTTC 1 cut(s) 840
PcsI WCGNNNNNNNCGW 2 cut(s) 706, 881
PfeI GAWTC 2 cut(s) 633, 1166
PflFI GACNNNGTC 1 cut(s) 883
PflMI CCANNNNNTGG 1 cut(s) 599
PkrI GCNGC 1 cut(s) 425
PleI GAGTC 2 cut(s) 302, 700
PpsI GAGTC 2 cut(s) 302, 700
Ppu21I YACGTR 1 cut(s) 815
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 3 cut(s) 333, 740, 828
PspPI GGNCC 2 cut(s) 331, 718
PsuI RGATCY 1 cut(s) 572
PsyI GACNNNGTC 1 cut(s) 883
PteI GCGCGC 1 cut(s) 1249
RruI TCGCGA 1 cut(s) 872
RsaI GTAC 3 cut(s) 274, 817, 965
RsaNI GTAC 3 cut(s) 273, 816, 964
RseI CAYNNNNRTG 2 cut(s) 110, 116
SacI GAGCTC 1 cut(s) 538
SalI GTCGAC 2 cut(s) 380, 953
SapI GCTCTTC 1 cut(s) 840
SaqAI TTAA 3 cut(s) 183, 206, 1200
SatI GCNGC 1 cut(s) 424
Sau3AI GATC 3 cut(s) 88, 572, 1185
Sau96I GGNCC 2 cut(s) 331, 718
SchI GAGTC 2 cut(s) 303, 700
ScrFI CCNGG 2 cut(s) 179, 594
SduI GDGCHC 2 cut(s) 538, 846
SfaNI GCATC 3 cut(s) 39, 928, 1264
SfcI CTRYAG 1 cut(s) 529
SgrDI CGTCGACG 1 cut(s) 953
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 110, 116
SnaBI TACGTA 1 cut(s) 815
SpeI ACTAGT 2 cut(s) 140, 475
Sse9I AATT 2 cut(s) 1147, 1201
SsiI CCGC 5 cut(s) 171, 221, 423, 566, 1019
SspMI CTAG 6 cut(s) 141, 174, 224, 476, 1131, 1176
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 2 cut(s) 177, 592
TaaI ACNGT 2 cut(s) 213, 524
TaiI ACGT 7 cut(s) 318, 560, 657, 686, 817, 887, 1142
TaqI TCGA 7 cut(s) 87, 240, 285, 381, 714, 954, 1188
TasI AATT 2 cut(s) 1147, 1201
TatI WGTACW 1 cut(s) 963
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 2 cut(s) 633, 1166
Tru1I TTAA 3 cut(s) 183, 206, 1200
Tru9I TTAA 3 cut(s) 183, 206, 1200
TseFI GTSAC 1 cut(s) 471
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 4 cut(s) 17, 69, 100, 1150
TspGWI ACGGA 2 cut(s) 267, 1068
Tth111I GACNNNGTC 1 cut(s) 883
Van91I CCANNNNNTGG 1 cut(s) 599
VpaK11BI GGWCC 1 cut(s) 331
XapI RAATTY 1 cut(s) 1147
XceI RCATGY 1 cut(s) 998
XcmI CCANNNNNNNNNTGG 1 cut(s) 547
XmiI GTMKAC 2 cut(s) 381, 954
XspI CTAG 6 cut(s) 141, 174, 224, 476, 1131, 1176
ZraI GACGTC 2 cut(s) 684, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.