Rw5G018010

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000852
Physical Location & Seq
Reverse (-)
23198181 .. 23199512
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018010.1

Sequence Viewer

Length: 1332 bp
ATGAACTTTCAAGTGGAGGTTCTCTCAACAGAAACAATCAAACCCTCATCTCCAACCCCTGACCACCTCCGCCACCACAAGCTCTCCTACCTCGACCAAATTCAGCCCCCAATCTTCATGCCCATGATCCTCTTCTACCCGAGAGAACCCGACCACAACGACCTGGACCTCCTTTCCATCCACGAACAGCGTTGCAGCAAAATCAAGAAGGCCTTATCCGACACCTTAGTCAAGTTCTACCCACTAGCCGGAAGAGTCCACGGAACCCAATACGTCGATTGCAACGACGAAGGAGCCTACTACGTCGAAGCCAAGACCAACTGCAAAATCTCTGACATCATCGACAACCCAAACCCTAACGACTTCAACAAGTTCCTTCCTTTCGAGCTAGACGCAGCTCATGAGCTTCCCGTGTGCTTCCAGGTCACCTTCTTCGCCTGCGGCGGCATGAGCATCGCCATGGGGATGTCCCACAAGGTCGGGGATGCCCTCTCCTACTTCACTTTTCTCAATGGCCTGGCCGCTGAGGCTCGTGGGGAGGGCAACGATATTCACCCTCCCGATTTCATCTCAGACAAGTACTTCCCCCAAAAGGACTTATCCGGATTCTTCCAACACCGTAGCGGGATCATAAAGCAAAACATCTCAACAAAACGCTTCGTCTTCGACGCTCCAATGGTTCAGGCCATCCGAGCCAAATGCGTCGACATGTCCAGACGCCCTACTCGCGTTGAAGCTCTATCAGCCTTCATATGGAGCCGCTACATAGCATCATGCACCGGCATGCAACAACATGGTGTCACCACCAACGCGATGTACACTGTTTCTCATGCTGTGAACCTCCGCACGAGAATGGAGCCACCACTTCCGGAGAACACATTCGGAAACGTGACCCGAACCGCCATTGCTGTGCCGCCTTTACCCGTTGACCCGAAAGACGGGACGCTTCATGGAGTGGTTAATCACGTTAGGGAGGCCATCAAGCAAGTAGACAAAGAGTACATAAAGAAACTGCAAGAGGGTGACGGACACTTGAGCTTCTTGAGGAGCCTTACGGAGGAGGTGAAACGCGGAGAGGTGGTGTCGTTTAGCTTCACGAGTCTGTGTAGGTTTCCGATCTACGACGCTGATTTTGGATGGGGAAAGCCTGTTTATGTTGGTTCTGCCAGCTTTACATTCAAGAACTTGGTTAGTTTCTTTGACACTAACGTAGGGGACGGAGTTGAGGCGTGGATCAATCTGTTGGAGGAAGACATGAAAAAATTCGAGGCGGACGAGGAGCTTCTCAAATATGTTTTGCCCGCCCCGAGTTCGAAGAATGCCAACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

443

Amino Acids

49.91

Weight (kDa)

6.11

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 428 2.2e-79 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccI GTMKAC 2 cut(s) 705, 990
AccII CGCG 3 cut(s) 729, 812, 1071
AccIII TCCGGA 2 cut(s) 602, 868
AclWI GGATC 3 cut(s) 121, 635, 1241
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 2 cut(s) 99, 1262
AcyI GRCGYC 1 cut(s) 718
AfaI GTAC 3 cut(s) 581, 818, 1001
AfiI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 4 cut(s) 11, 367, 734, 1180
AjnI CCWGG 3 cut(s) 162, 420, 516
AluBI AGCT 9 cut(s) 82, 388, 398, 406, 737, 1038, 1092, 1170, 1282
AluI AGCT 9 cut(s) 82, 388, 398, 406, 737, 1038, 1092, 1170, 1282
AlwI GGATC 3 cut(s) 121, 635, 1241
Ama87I CYCGRG 2 cut(s) 139, 1306
Aor13HI TCCGGA 2 cut(s) 602, 868
AoxI GGCC 5 cut(s) 210, 514, 519, 684, 975
ApeKI GCWGC 2 cut(s) 195, 395
ApoI RAATTY 2 cut(s) 99, 1262
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
Asp700I GAANNNNTTC 2 cut(s) 878, 1262
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 5 cut(s) 418, 545, 793, 1034, 1075
AsuII TTCGAA 1 cut(s) 1313
AvaI CYCGRG 2 cut(s) 139, 1306
AvaII GGWCC 1 cut(s) 166
BauI CACGAG 3 cut(s) 531, 847, 1096
BbsI GAAGAC 2 cut(s) 655, 1257
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 207, 407
BccI CCATC 4 cut(s) 185, 695, 986, 1131
BcgI CGANNNNNNTGC 4 cut(s) 436, 470, 681, 715
BciT130I CCWGG 3 cut(s) 164, 422, 518
BfaI CTAG 2 cut(s) 245, 389
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
BlsI GCNGC 7 cut(s) 197, 397, 443, 446, 523, 761, 915
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 3 cut(s) 164, 422, 518
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 2 cut(s) 139, 1306
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
BmrFI CCNGG 3 cut(s) 164, 422, 518
BmsI GCATC 3 cut(s) 462, 475, 779
BpiI GAAGAC 2 cut(s) 655, 1257
BplI GAGNNNNNCTC 2 cut(s) 8, 40
Bpu10I CCTNAGC 1 cut(s) 525
Bpu14I TTCGAA 1 cut(s) 1313
BpuEI CTTGAG 2 cut(s) 1054, 1063
BsaHI GRCGYC 1 cut(s) 718
BsaJI CCNNGG 2 cut(s) 259, 459
BsaWI WCCGGW 2 cut(s) 602, 868
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
Bse118I RCCGGY 1 cut(s) 779
Bse3DI GCAATG 1 cut(s) 903
BseAI TCCGGA 2 cut(s) 602, 868
BseBI CCWGG 3 cut(s) 164, 422, 518
BseDI CCNNGG 2 cut(s) 259, 459
BseGI GGATG 5 cut(s) 177, 471, 490, 687, 1142
BseLI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 2 cut(s) 516, 585
BseRI GAGGAG 3 cut(s) 1060, 1073, 1292
BseXI GCAGC 2 cut(s) 207, 407
Bsh1236I CGCG 3 cut(s) 729, 812, 1071
BshFI GGCC 5 cut(s) 212, 516, 521, 686, 977
BsiHKCI CYCGRG 2 cut(s) 139, 1306
BsiSI CCGG 4 cut(s) 249, 603, 780, 869
BslFI GGGAC 3 cut(s) 454, 955, 1229
BslI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1057
BsmFI GGGAC 3 cut(s) 454, 955, 1229
BsmI GAATGC 1 cut(s) 1324
BsnI GGCC 5 cut(s) 212, 516, 521, 686, 977
BsoBI CYCGRG 2 cut(s) 139, 1306
Bsp119I TTCGAA 1 cut(s) 1313
Bsp13I TCCGGA 2 cut(s) 602, 868
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 4 cut(s) 126, 627, 1116, 1233
Bsp19I CCATGG 1 cut(s) 459
BspANI GGCC 5 cut(s) 212, 516, 521, 686, 977
BspCNI CTCAG 2 cut(s) 517, 584
BspEI TCCGGA 2 cut(s) 602, 868
BspFNI CGCG 3 cut(s) 729, 812, 1071
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
BspPI GGATC 3 cut(s) 121, 635, 1241
BspT104I TTCGAA 1 cut(s) 1313
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 779
BsrGI TGTACA 1 cut(s) 816
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 2 cut(s) 259, 459
BssMI GATC 4 cut(s) 126, 627, 1116, 1233
BssNI GRCGYC 1 cut(s) 718
BssSI CACGAG 3 cut(s) 531, 847, 1096
BssT1I CCWWGG 1 cut(s) 459
Bst2BI CACGAG 3 cut(s) 531, 847, 1096
Bst2UI CCWGG 3 cut(s) 164, 422, 518
Bst4CI ACNGT 2 cut(s) 620, 823
Bst6I CTCTTC 2 cut(s) 137, 247
BstACI GRCGYC 1 cut(s) 718
BstAUI TGTACA 1 cut(s) 816
BstBI TTCGAA 1 cut(s) 1313
BstC8I GCNNGC 4 cut(s) 439, 785, 1168, 1302
BstDEI CTNAG 3 cut(s) 226, 525, 571
BstDSI CCRYGG 2 cut(s) 259, 459
BstEII GGTNACC 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 1055
BstF5I GGATG 5 cut(s) 177, 471, 490, 687, 1142
BstFNI CGCG 3 cut(s) 729, 812, 1071
BstKTI GATC 4 cut(s) 129, 630, 1119, 1236
BstMBI GATC 4 cut(s) 126, 627, 1116, 1233
BstMWI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
BstNI CCWGG 3 cut(s) 164, 422, 518
BstNSI RCATGY 2 cut(s) 712, 787
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 162, 420, 516
BstUI CGCG 3 cut(s) 729, 812, 1071
BstV1I GCAGC 2 cut(s) 207, 407
BstV2I GAAGAC 2 cut(s) 655, 1257
BsuRI GGCC 5 cut(s) 212, 516, 521, 686, 977
BtgI CCRYGG 2 cut(s) 259, 459
BtgZI GCGATG 2 cut(s) 439, 827
BtsCI GGATG 5 cut(s) 177, 471, 490, 687, 1142
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 4 cut(s) 439, 785, 1168, 1302
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 1 cut(s) 166
CseI GACGC 6 cut(s) 401, 677, 691, 726, 952, 1133
Csp6I GTAC 3 cut(s) 580, 817, 1000
CviQI GTAC 3 cut(s) 580, 817, 1000
DdeI CTNAG 3 cut(s) 226, 525, 571
DpnI GATC 4 cut(s) 128, 629, 1118, 1235
DpnII GATC 4 cut(s) 126, 627, 1116, 1233
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 137, 247
EarI CTCTTC 2 cut(s) 137, 247
EciI GGCGGA 2 cut(s) 59, 1286
Eco130I CCWWGG 1 cut(s) 459
Eco147I AGGCCT 1 cut(s) 212
Eco47I GGWCC 1 cut(s) 166
Eco88I CYCGRG 2 cut(s) 139, 1306
Eco91I GGTNACC 1 cut(s) 424
EcoNI CCTNNNNNAGG 1 cut(s) 1055
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 162, 420, 516
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 3 cut(s) 454, 955, 1229
FauI CCCGC 2 cut(s) 617, 1309
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 2 cut(s) 705, 990
Fnu4HI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
FokI GGATG 5 cut(s) 164, 478, 497, 674, 1149
Fsp4HI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
FspBI CTAG 2 cut(s) 245, 389
GluI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
HaeIII GGCC 5 cut(s) 212, 516, 521, 686, 977
HapII CCGG 4 cut(s) 249, 603, 780, 869
HgaI GACGC 6 cut(s) 401, 677, 691, 726, 952, 1133
Hin1I GRCGYC 1 cut(s) 718
HincII GTYRAC 2 cut(s) 706, 928
HindII GTYRAC 2 cut(s) 706, 928
HinfI GANTC 3 cut(s) 255, 606, 1099
HpaII CCGG 4 cut(s) 249, 603, 780, 869
HphI GGTGA 5 cut(s) 418, 545, 793, 1034, 1075
Hpy166II GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy188I TCNGA 6 cut(s) 220, 334, 574, 692, 884, 1116
Hpy188III TCNNGA 9 cut(s) 205, 401, 560, 603, 714, 869, 1042, 1096, 1180
Hpy8I GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy99I CGWCG 6 cut(s) 278, 290, 308, 671, 707, 1127
HpyAV CCTTC 5 cut(s) 202, 284, 386, 439, 757
HpyCH4III ACNGT 2 cut(s) 620, 823
HpyCH4IV ACGT 5 cut(s) 273, 303, 888, 966, 1209
HpyCH4V TGCA 6 cut(s) 195, 282, 324, 777, 787, 1015
HpyF10VI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
HpyF3I CTNAG 3 cut(s) 226, 525, 571
HpySE526I ACGT 5 cut(s) 273, 303, 888, 966, 1209
Hsp92I GRCGYC 1 cut(s) 718
Kpn2I TCCGGA 2 cut(s) 602, 868
Kzo9I GATC 4 cut(s) 126, 627, 1116, 1233
LmnI GCTCC 6 cut(s) 293, 676, 756, 856, 1047, 1279
Lsp1109I GCAGC 2 cut(s) 207, 407
LweI GCATC 3 cut(s) 462, 475, 779
MaeI CTAG 2 cut(s) 245, 389
MaeII ACGT 5 cut(s) 273, 303, 888, 966, 1209
MaeIII GTNAC 4 cut(s) 424, 799, 889, 1022
MalI GATC 4 cut(s) 128, 629, 1118, 1235
MboI GATC 4 cut(s) 126, 627, 1116, 1233
MboII GAAGA 8 cut(s) 106, 124, 264, 424, 601, 655, 1262, 1327
MluCI AATT 2 cut(s) 99, 1262
MlyI GAGTC 2 cut(s) 264, 1108
MmeI TCCRAC 4 cut(s) 77, 243, 637, 1224
MroI TCCGGA 2 cut(s) 602, 868
MroXI GAANNNNTTC 2 cut(s) 878, 1262
MseI TTAA 1 cut(s) 960
MslI CAYNNNNRTG 6 cut(s) 122, 458, 464, 782, 851, 908
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 4 cut(s) 249, 603, 780, 869
MspR9I CCNGG 3 cut(s) 164, 422, 518
Mva1269I GAATGC 1 cut(s) 1324
MvaI CCWGG 3 cut(s) 164, 422, 518
MvnI CGCG 3 cut(s) 729, 812, 1071
MwoI GCNNNNNNNGC 5 cut(s) 450, 527, 692, 726, 743
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 752
NdeII GATC 4 cut(s) 126, 627, 1116, 1233
NlaIV GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
NmuCI GTSAC 4 cut(s) 424, 799, 889, 1022
NspI RCATGY 2 cut(s) 712, 787
NspV TTCGAA 1 cut(s) 1313
PaeI GCATGC 1 cut(s) 787
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 1 cut(s) 212
PciI ACATGT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 1272
PctI GAATGC 1 cut(s) 1324
PdmI GAANNNNTTC 2 cut(s) 878, 1262
PfeI GAWTC 1 cut(s) 606
PkrI GCNGC 7 cut(s) 197, 397, 443, 446, 523, 761, 915
PleI GAGTC 2 cut(s) 263, 1107
PpsI GAGTC 2 cut(s) 263, 1107
PscI ACATGT 1 cut(s) 708
Psp6I CCWGG 3 cut(s) 162, 420, 516
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 3 cut(s) 162, 420, 516
PspN4I GGNNCC 5 cut(s) 265, 295, 758, 858, 1049
PspPI GGNCC 1 cut(s) 166
RsaI GTAC 3 cut(s) 581, 818, 1001
RsaNI GTAC 3 cut(s) 580, 817, 1000
RseI CAYNNNNRTG 6 cut(s) 122, 458, 464, 782, 851, 908
SalI GTCGAC 1 cut(s) 704
SaqAI TTAA 1 cut(s) 960
SatI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
Sau3AI GATC 4 cut(s) 126, 627, 1116, 1233
Sau96I GGNCC 1 cut(s) 166
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 2 cut(s) 264, 1108
ScrFI CCNGG 3 cut(s) 164, 422, 518
SfaNI GCATC 3 cut(s) 462, 475, 779
SfuI TTCGAA 1 cut(s) 1313
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 6 cut(s) 122, 458, 464, 782, 851, 908
SmlI CTYRAG 2 cut(s) 1033, 1042
SmoI CTYRAG 2 cut(s) 1033, 1042
SphI GCATGC 1 cut(s) 787
Sse9I AATT 2 cut(s) 99, 1262
SseBI AGGCCT 1 cut(s) 212
SspMI CTAG 2 cut(s) 245, 389
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 3 cut(s) 162, 420, 516
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 2 cut(s) 620, 823
TaiI ACGT 5 cut(s) 276, 306, 891, 969, 1212
TaqI TCGA 9 cut(s) 93, 276, 306, 342, 384, 666, 705, 1266, 1313
TasI AATT 2 cut(s) 99, 1262
TatI WGTACW 3 cut(s) 579, 816, 999
TauI GCSGC 5 cut(s) 444, 447, 524, 762, 916
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 4 cut(s) 424, 799, 889, 1022
TseI GCWGC 2 cut(s) 195, 395
Tsp45I GTSAC 4 cut(s) 424, 799, 889, 1022
TspDTI ATGAA 6 cut(s) 17, 106, 556, 739, 938, 1271
TspGWI ACGGA 4 cut(s) 276, 1041, 1070, 1233
TspRI CASTG 1 cut(s) 826
VpaK11BI GGWCC 1 cut(s) 166
XagI CCTNNNNNAGG 1 cut(s) 1055
XapI RAATTY 2 cut(s) 99, 1262
XceI RCATGY 2 cut(s) 712, 787
XmiI GTMKAC 2 cut(s) 705, 990
XmnI GAANNNNTTC 2 cut(s) 878, 1262
XspI CTAG 2 cut(s) 245, 389
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.