RLG00000006888

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000852
Physical Location & Seq
Forward (+)
11542281 .. 11543950
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006888

Sequence Viewer

Length: 1338 bp
ATGATCATGAAGGTTGAGGTTATCTCCAAAGAGTCTATCAGACCTTCTTCTCCAACCCCTGACCACCTCCACAGATACCAACTCTCCTTTCTTGACCAGATATCTCCCCCAGTCTTTAGTCCTTTGGTTCTATTTTATTCATCATCCGAGGCTGATACAAATTTCGACATTGCTGATATATCGAATCGGCTAAAGAAATCCCTCTCCGATGTCTTGGTCCAGTTCTACCCACTCGCTGGACGAGCAAATGACAACATGTTCATAGACTGCAACGACAAGGGCATACCCTTCTTGGAAACTCGAGTTAACTGCCAACTATCTGATGTTATCCAAAACCCAGTTCCCAGTGAACTCAACAAGTTGGTTCCATTTCAACTAGACGAAGTTGCTGATCTAGCCCTTGGTGTCCAACTAAATGTCTTTGACTGTGGTGGAGTTGCAATTGGTGCTTGCATTTCTCACAAGATTGCAGATGCTTTATCGTTCTTCATGTTCATCAAAACTTGGGCAGCCACTGCTAGAAAAGATAATTATCATCAGACAGATGATCATACAGTCATCTCTCCACAGTTTGTGTCAGCCACACTGTTCCCACCAAGGAACATATCCGGGTTTAATCCACGTGTTGGCATCACAAAAGAGAATATCGTAACAAAGAGGTTTGTGTTTGATGCTTCCAAGATAGATTCTCTTAAATCAAAATATGGAGTTAATGATAATGATCATGAGCATGAGCAGAGCCTAGAAAGTTACCAAAGACCGCCATCTCGTGTGGAGGCTCTATCCGCTTTCATATGGGGTCGCTATGTGGCTGCAACCAAAGTTGGGTCTGAAGCACCTGAGAGGGTCTATGCCATTGTTCATGCTGTGAACCTCAGGACAAGGTTTGATCCACCACTGCCAGAATGCTCTTTCGGAAATCTCTACCGAATTGCAGTTACGGTTCCACGCCTGGATACAGGGGAGGAGTGCTACGGTCTCGTGAGGCAGGTCAGAGAGCAAGTGAGTCATATTGACAAGGATTATGTGAAGAAATTGCAGGAGGGCAGTGAACACTTGAGTTTCATTAAACAAAGCTCTGAGAATTTCTACAAGGGAGAGATGGTTACACTGAGCTTCACTAGTTTGTGCAGATTTCCTCTGTATGAAGCTGATTTTGGATGGGGAAAGCCTACTTGGGTCGGCTCTCCTGCCCTGACTTTCAATAATTTGGTTGTTTTTATAGACACTAAATCTGGGGGTGGAATAGAAGCATACATTAACATGAAGGAAGGAGATTTGGCTAAAATTGAATCTGACGAAGAGTTCATTTCATTTGTTTCAAATGAGTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

446

Amino Acids

49.94

Weight (kDa)

5.4

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 436 2e-81 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 979
AccB7I CCANNNNNTGG 2 cut(s) 236, 626
AciI CCGC 2 cut(s) 761, 786
AclWI GGATC 1 cut(s) 884
AcsI RAATTY 2 cut(s) 160, 1084
AcuI CTGAAG 1 cut(s) 852
AcvI CACGTG 1 cut(s) 623
AfiI CCNNNNNNNGG 3 cut(s) 236, 626, 825
AflIII ACRYGT 2 cut(s) 255, 622
AgsI TTSAA 4 cut(s) 374, 1204, 1292, 1323
AhlI ACTAGT 1 cut(s) 1121
AjnI CCWGG 1 cut(s) 951
AjuI GAANNNNNNNTTGG 8 cut(s) 72, 104, 324, 356, 1140, 1159, 1172, 1191
AluBI AGCT 3 cut(s) 1077, 1116, 1151
AluI AGCT 3 cut(s) 1077, 1116, 1151
Alw26I GTCTC 1 cut(s) 983
AlwI GGATC 1 cut(s) 884
AlwNI CAGNNNCTG 1 cut(s) 515
Ama87I CYCGRG 1 cut(s) 300
ApeKI GCWGC 2 cut(s) 509, 812
ApoI RAATTY 2 cut(s) 160, 1084
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 1 cut(s) 217
AxyI CCTNAGG 1 cut(s) 875
BauI CACGAG 2 cut(s) 768, 980
BbrPI CACGTG 1 cut(s) 623
BbvI GCAGC 2 cut(s) 521, 799
BccI CCATC 3 cut(s) 772, 1096, 1155
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BciT130I CCWGG 1 cut(s) 953
BciVI GTATCC 1 cut(s) 949
BclI TGATCA 3 cut(s) 3, 547, 721
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 1 cut(s) 983
BcuI ACTAGT 1 cut(s) 1121
BfaI CTAG 5 cut(s) 377, 395, 519, 743, 1122
BfuAI ACCTGC 1 cut(s) 979
BfuI GTATCC 1 cut(s) 949
BisI GCNGC 2 cut(s) 510, 813
BlsI GCNGC 2 cut(s) 511, 814
Bme1390I CCNGG 2 cut(s) 610, 953
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 2 cut(s) 366, 945
BmrFI CCNGG 2 cut(s) 610, 953
BmrI ACTGGG 3 cut(s) 104, 332, 339
BmsI GCATC 3 cut(s) 463, 639, 661
BmuI ACTGGG 3 cut(s) 104, 332, 339
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpuEI CTTGAG 1 cut(s) 1078
BpuMI CCSGG 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 623
BsaBI GATNNNNATC 2 cut(s) 531, 720
BsaI GGTCTC 1 cut(s) 983
BsaJI CCNNGG 3 cut(s) 147, 400, 596
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1089, 1119
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 626, 825
Bse1I ACTGG 4 cut(s) 110, 220, 338, 345
Bse21I CCTNAGG 1 cut(s) 875
Bse3DI GCAATG 1 cut(s) 168
Bse8I GATNNNNATC 2 cut(s) 531, 720
BseBI CCWGG 1 cut(s) 953
BseDI CCNNGG 3 cut(s) 147, 400, 596
BseGI GGATG 2 cut(s) 143, 1166
BseJI GATNNNNATC 2 cut(s) 531, 720
BseLI CCNNNNNNNGG 3 cut(s) 236, 626, 825
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 4 cut(s) 831, 889, 1071, 1103
BseNI ACTGG 4 cut(s) 110, 220, 338, 345
BseRI GAGGAG 1 cut(s) 980
BseXI GCAGC 2 cut(s) 521, 799
BsgI GTGCAG 1 cut(s) 1150
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 1 cut(s) 609
BslI CCNNNNNNNGG 3 cut(s) 236, 626, 825
BsmAI GTCTC 1 cut(s) 983
BsmI GAATGC 1 cut(s) 911
Bso31I GGTCTC 1 cut(s) 983
BsoBI CYCGRG 1 cut(s) 300
Bsp143I GATC 5 cut(s) 3, 391, 547, 721, 889
BspACI CCGC 2 cut(s) 761, 786
BspCNI CTCAG 4 cut(s) 832, 888, 1072, 1104
BspHI TCATGA 2 cut(s) 6, 724
BspLI GGNNCC 2 cut(s) 366, 945
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 1 cut(s) 884
BspTNI GGTCTC 1 cut(s) 983
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 4 cut(s) 110, 220, 338, 345
BssECI CCNNGG 3 cut(s) 147, 400, 596
BssMI GATC 5 cut(s) 3, 391, 547, 721, 889
BssSI CACGAG 2 cut(s) 768, 980
BssT1I CCWWGG 2 cut(s) 400, 596
Bst2BI CACGAG 2 cut(s) 768, 980
Bst2UI CCWGG 1 cut(s) 953
Bst4CI ACNGT 6 cut(s) 428, 556, 570, 588, 943, 977
Bst6I CTCTTC 1 cut(s) 1296
BstAPI GCANNNNNTGC 2 cut(s) 446, 515
BstBAI YACGTR 1 cut(s) 623
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 4 cut(s) 840, 875, 1080, 1112
BstF5I GGATG 2 cut(s) 143, 1166
BstKTI GATC 5 cut(s) 6, 394, 550, 724, 892
BstMAI GTCTC 1 cut(s) 983
BstMBI GATC 5 cut(s) 3, 391, 547, 721, 889
BstMWI GCNNNNNNNGC 5 cut(s) 242, 395, 446, 515, 785
BstNI CCWGG 1 cut(s) 953
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 608, 951
BstV1I GCAGC 2 cut(s) 521, 799
Bsu36I CCTNAGG 1 cut(s) 875
BsuI GTATCC 1 cut(s) 949
BtsCI GGATG 2 cut(s) 143, 1166
BtsI GCAGTG 3 cut(s) 513, 896, 1054
BtsIMutI CAGTG 6 cut(s) 352, 513, 584, 896, 1054, 1109
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 1 cut(s) 451
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 2 cut(s) 6, 724
Cfr13I GGNCC 1 cut(s) 217
CviAII CATG 7 cut(s) 7, 256, 490, 725, 731, 863, 1264
DdeI CTNAG 4 cut(s) 840, 875, 1080, 1112
DpnI GATC 5 cut(s) 5, 393, 549, 723, 891
DpnII GATC 5 cut(s) 3, 391, 547, 721, 889
Eam1104I CTCTTC 1 cut(s) 1296
EarI CTCTTC 1 cut(s) 1296
Eco130I CCWWGG 2 cut(s) 400, 596
Eco31I GGTCTC 1 cut(s) 983
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 852
Eco72I CACGTG 1 cut(s) 623
Eco81I CCTNAGG 1 cut(s) 875
Eco88I CYCGRG 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 951
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 2 cut(s) 400, 596
ErhI CCWWGG 2 cut(s) 400, 596
FaeI CATG 7 cut(s) 10, 259, 493, 728, 734, 866, 1267
FatI CATG 7 cut(s) 6, 255, 489, 724, 730, 862, 1263
FauNDI CATATG 1 cut(s) 794
FbaI TGATCA 3 cut(s) 3, 547, 721
Fnu4HI GCNGC 2 cut(s) 510, 813
FokI GGATG 2 cut(s) 130, 1173
Fsp4HI GCNGC 2 cut(s) 510, 813
FspBI CTAG 5 cut(s) 377, 395, 519, 743, 1122
GluI GCNGC 2 cut(s) 510, 813
HapII CCGG 1 cut(s) 609
Hin1II CATG 7 cut(s) 10, 259, 493, 728, 734, 866, 1267
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 6 cut(s) 32, 184, 686, 1006, 1292, 1328
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 1 cut(s) 609
Hpy166II GTNNAC 4 cut(s) 307, 350, 871, 1052
Hpy188III TCNNGA 5 cut(s) 7, 92, 725, 877, 982
Hpy8I GTNNAC 4 cut(s) 307, 350, 871, 1052
HpyAV CCTTC 5 cut(s) 4, 54, 298, 1261, 1265
HpyCH4III ACNGT 6 cut(s) 428, 556, 570, 588, 943, 977
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 8 cut(s) 270, 440, 453, 470, 815, 935, 1039, 1131
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 395, 446, 515, 785
HpyF3I CTNAG 4 cut(s) 840, 875, 1080, 1112
HpySE526I ACGT 1 cut(s) 622
Hsp92II CATG 7 cut(s) 10, 259, 493, 728, 734, 866, 1267
Ksp22I TGATCA 3 cut(s) 3, 547, 721
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 5 cut(s) 3, 391, 547, 721, 889
Lsp1109I GCAGC 2 cut(s) 521, 799
LweI GCATC 3 cut(s) 463, 639, 661
MaeI CTAG 5 cut(s) 377, 395, 519, 743, 1122
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 4 cut(s) 649, 749, 937, 1105
MalI GATC 5 cut(s) 5, 393, 549, 723, 891
MboI GATC 5 cut(s) 3, 391, 547, 721, 889
MboII GAAGA 4 cut(s) 39, 478, 1042, 1313
MfeI CAATTG 1 cut(s) 441
MluCI AATT 8 cut(s) 160, 441, 529, 930, 1034, 1084, 1207, 1287
MlyI GAGTC 3 cut(s) 41, 1015, 1337
MmeI TCCRAC 2 cut(s) 77, 433
MseI TTAA 6 cut(s) 306, 615, 693, 711, 1068, 1260
MslI CAYNNNNRTG 2 cut(s) 729, 1262
MspI CCGG 1 cut(s) 609
MspR9I CCNGG 2 cut(s) 610, 953
MunI CAATTG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 911
MvaI CCWGG 1 cut(s) 953
MwoI GCNNNNNNNGC 5 cut(s) 242, 395, 446, 515, 785
NciI CCSGG 1 cut(s) 610
NdeI CATATG 1 cut(s) 794
NdeII GATC 5 cut(s) 3, 391, 547, 721, 889
NlaIII CATG 7 cut(s) 10, 259, 493, 728, 734, 866, 1267
NlaIV GGNNCC 2 cut(s) 366, 945
NspI RCATGY 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 300
PagI TCATGA 2 cut(s) 6, 724
PciI ACATGT 1 cut(s) 255
PctI GAATGC 1 cut(s) 911
PfeI GAWTC 3 cut(s) 184, 686, 1292
PflMI CCANNNNNTGG 2 cut(s) 236, 626
PkrI GCNGC 2 cut(s) 511, 814
PleI GAGTC 3 cut(s) 40, 1014, 1336
PmaCI CACGTG 1 cut(s) 623
PmlI CACGTG 1 cut(s) 623
PpsI GAGTC 3 cut(s) 40, 1014, 1336
Ppu21I YACGTR 1 cut(s) 623
PscI ACATGT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 951
PspCI CACGTG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 951
PspN4I GGNNCC 2 cut(s) 366, 945
PspPI GGNCC 1 cut(s) 217
PspXI VCTCGAGB 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 515
RseI CAYNNNNRTG 2 cut(s) 729, 1262
SaqAI TTAA 6 cut(s) 306, 615, 693, 711, 1068, 1260
SatI GCNGC 2 cut(s) 510, 813
Sau3AI GATC 5 cut(s) 3, 391, 547, 721, 889
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 3 cut(s) 41, 1015, 1337
ScrFI CCNGG 2 cut(s) 610, 953
SfaNI GCATC 3 cut(s) 463, 639, 661
Sfr274I CTCGAG 1 cut(s) 300
SinI GGWCC 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 300
SmiMI CAYNNNNRTG 2 cut(s) 729, 1262
SmlI CTYRAG 2 cut(s) 300, 1057
SmoI CTYRAG 2 cut(s) 300, 1057
SpeI ACTAGT 1 cut(s) 1121
Sse9I AATT 8 cut(s) 160, 441, 529, 930, 1034, 1084, 1207, 1287
SsiI CCGC 2 cut(s) 761, 786
SspMI CTAG 5 cut(s) 377, 395, 519, 743, 1122
StyD4I CCNGG 2 cut(s) 608, 951
StyI CCWWGG 2 cut(s) 400, 596
TaaI ACNGT 6 cut(s) 428, 556, 570, 588, 943, 977
TaiI ACGT 1 cut(s) 625
TaqI TCGA 3 cut(s) 165, 182, 301
TasI AATT 8 cut(s) 160, 441, 529, 930, 1034, 1084, 1207, 1287
TfiI GAWTC 3 cut(s) 184, 686, 1292
Tru1I TTAA 6 cut(s) 306, 615, 693, 711, 1068, 1260
Tru9I TTAA 6 cut(s) 306, 615, 693, 711, 1068, 1260
TscAI CASTG 6 cut(s) 352, 520, 591, 903, 1054, 1116
TseI GCWGC 2 cut(s) 509, 812
TspRI CASTG 6 cut(s) 352, 520, 591, 903, 1054, 1116
Van91I CCANNNNNTGG 2 cut(s) 236, 626
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 2 cut(s) 160, 1084
XceI RCATGY 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 300
XspI CTAG 5 cut(s) 377, 395, 519, 743, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.