pycom13g09450

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000852
Physical Location & Seq
Forward (+)
6328669 .. 6331032
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g09450.8

Sequence Viewer

Length: 1674 bp
ATGAAAGATAGTCTACAAAATAAAGTACAGCTTAACACTATCACTATCACCTTTTCCACTGGCTCCAACTTGACACCTTATCACCTTATCACTATCACTATCACTATCAACTTATCCACTTGCGCCAAAAGCATTACAACGTTAACTGCATTTAGCTTGAGGAAAACAAGAGGGCCTGCTGGTTTGGCACCACTCGAGCCGACGAGCACTATAAATTTGACACTCCCTAACCAAATAATCCACAACACCATCTGCTGCAGTAGCTGCATACAACTCTTTCACATATCAAAGAAGATAGAAGTAATTGAGAAAGCTTGCGTTAAGCCTTCTATGCCAACCCCACCCCACCTTCGTAAACACGAGTTTTCTTTTCTCGACCAAATCGCAACTCCCATTTTCATGTTAAAACAATCCTTATCCAAGACCCTGACACAATATTATCCGCTAGCCGGACGTGCCATTGAAAATGCCTACGTCGATTGCAATGACCAAGGTGCCCCTTACGTCCAAGCCCGAGTTAGGTCCCCACTTTCTGATGTCATATCCCAACCCGACCCAAACCAGCTCAACCAACTACTCCCATGCGAACTAGACAACGTCGGTGACTTGGTTCTTGCTGTCCAAGTCAACGTGTTCGATTGTGGTGGCATGGCTATTGGAGTTTGCATTTCTCACAAAGTTGCCGATGCATTGTCATTCATCATGTTCCTCAACAGTTGGGCCTCGATAACTCGTGGAGATTGCAACATTAAAAATAGTACAGTCAGTCCTCCAATTTTTGATTTGGCCACGCACTTTCCACCTAGAAGCATATCAGGGTTCAAACCAAGCACTGGGATCACTAGAGAAAAAATTGTTACAAAAAGGTTTGTTTTCAGTGCTTCCACGGTTGCTTCTCTTAGGTCAAAATATACAGATAATAAAAACAATGAGAGAAAGCCAACTCGGATTGAGGCCTTGTCAGCATTTATATGGGCCCGGGTTATTGCCTCCACTCAAGGAAAGCCGAACCCTAACACTCTTTACAAAGTGCTTCAAGCCGTTACCTCACGGACGAGGATCGATCCACCACTGCCCGAATACCATTTCGGGAACGTGAGCCGGTTTGCGATGTCTTCGCCGTCGTTTGATGAGGGAGAGGATATGTGTTACGGGATGGTGGGCCAGATGAGGGAAGCAATAAAGACAATCGACGGTGATTACAGTGCCAAAATCAAAGACGGCGACGCGCACTTGAAGTTTTTGAAGCAGCGAGCGGAGGAGTTTATGAAGGGTGAAGTGGCGTCGTTTAGCTTTACCAGCTTGTGCAGTTTCCCACTTTATGAAACGGACTTCGGGTGGAGCAACCCTGTGTGGGTCGCGTCGGCCAGCCTTCCATTTAAGAACCTGGTTGTTTTCATGGATGCTGGGTCGAGTGGTGGGATTGAAGTGTGGGTGAATTTGAAGGAGGAAGACATGGCTAAGTTCCAAGATGATGAGCAACTGTTGGCATGTACTTATCCAGCCCAAGAGGCTAAGTTTCTTACTAATTCGTCTTGTATAATACCAAGGTTGAATAATTTACTTTGTCCTTATTTACAGTCTAATACTTATCTTACGGTGATATGTAGGTACATGTTATTTTTGTTGTGTGGAAAAGATGCTTCATTCAAAAGGAAACGCCACGCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

558

Amino Acids

61.88

Weight (kDa)

8.7

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 187, 494
AccB7I CCANNNNNTGG 1 cut(s) 833
AccBSI CCGCTC 1 cut(s) 1256
AccI GTMKAC 1 cut(s) 13
AccII CGCG 2 cut(s) 1229, 1361
AciI CCGC 2 cut(s) 443, 1256
AclI AACGTT 1 cut(s) 140
AclWI GGATC 3 cut(s) 845, 1058, 1067
AcoI YGGCCR 2 cut(s) 786, 1365
AcsI RAATTY 2 cut(s) 214, 1438
AcyI GRCGYC 1 cut(s) 1283
AfaI GTAC 4 cut(s) 27, 760, 1495, 1613
AfiI CCNNNNNNNGG 5 cut(s) 449, 519, 833, 1171, 1354
AflIII ACRYGT 2 cut(s) 630, 1614
AgsI TTSAA 9 cut(s) 464, 823, 1037, 1237, 1246, 1427, 1444, 1555, 1651
AjiI CACGTC 1 cut(s) 455
AjnI CCWGG 1 cut(s) 1386
AluBI AGCT 7 cut(s) 31, 156, 264, 314, 565, 1293, 1302
AluI AGCT 7 cut(s) 31, 156, 264, 314, 565, 1293, 1302
Alw21I GWGCWC 1 cut(s) 209
AlwI GGATC 3 cut(s) 845, 1058, 1067
AlwNI CAGNNNCTG 1 cut(s) 264
Ama87I CYCGRG 3 cut(s) 194, 513, 978
AoxI GGCC 7 cut(s) 173, 720, 786, 954, 975, 1162, 1365
ApaI GGGCCC 1 cut(s) 979
ApeKI GCWGC 3 cut(s) 255, 264, 1249
ApoI RAATTY 2 cut(s) 214, 1438
AspLEI GCGC 2 cut(s) 125, 1231
AspS9I GGNCC 6 cut(s) 173, 522, 720, 975, 976, 1162
AsuC2I CCSGG 2 cut(s) 979, 980
AsuHPI GGTGA 7 cut(s) 40, 74, 614, 1208, 1286, 1447, 1612
AsuNHI GCTAGC 1 cut(s) 445
AvaI CYCGRG 3 cut(s) 194, 513, 978
AvaII GGWCC 1 cut(s) 522
BaeGI GKGCMC 2 cut(s) 499, 979
BalI TGGCCA 1 cut(s) 788
BanI GGYRCC 2 cut(s) 187, 494
BanII GRGCYC 1 cut(s) 979
BauI CACGAG 2 cut(s) 359, 732
BbsI GAAGAC 2 cut(s) 1107, 1458
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 3 cut(s) 242, 251, 1261
BccI CCATC 2 cut(s) 257, 1150
BceAI ACGGC 3 cut(s) 1025, 1105, 1237
BciT130I CCWGG 1 cut(s) 1388
BcnI CCSGG 2 cut(s) 979, 980
BfaI CTAG 4 cut(s) 446, 590, 804, 843
BfmI CTRYAG 1 cut(s) 256
BglI GCCNNNNNGGC 1 cut(s) 1511
BisI GCNGC 3 cut(s) 256, 265, 1250
BlsI GCNGC 3 cut(s) 257, 266, 1251
Bme1390I CCNGG 3 cut(s) 979, 980, 1388
Bme18I GGWCC 1 cut(s) 522
BmeT110I CYCGRG 3 cut(s) 194, 513, 978
BmgBI CACGTC 1 cut(s) 455
BmgT120I GGNCC 6 cut(s) 173, 522, 720, 975, 976, 1162
BmiI GGNNCC 5 cut(s) 64, 189, 496, 524, 977
BmrFI CCNGG 3 cut(s) 979, 980, 1388
BmrI ACTGGG 1 cut(s) 843
BmsI GCATC 3 cut(s) 676, 1393, 1630
BmtI GCTAGC 1 cut(s) 449
BmuI ACTGGG 1 cut(s) 843
BpiI GAAGAC 2 cut(s) 1107, 1458
BpuEI CTTGAG 2 cut(s) 178, 981
BpuMI CCSGG 2 cut(s) 979, 980
Bsa29I ATCGAT 1 cut(s) 1062
BsaHI GRCGYC 1 cut(s) 1283
BsaJI CCNNGG 4 cut(s) 490, 885, 978, 1547
Bsc4I CCNNNNNNNGG 5 cut(s) 449, 519, 833, 1171, 1354
Bse118I RCCGGY 1 cut(s) 1101
Bse1I ACTGG 2 cut(s) 64, 838
Bse3DI GCAATG 1 cut(s) 490
BseBI CCWGG 1 cut(s) 1388
BseCI ATCGAT 1 cut(s) 1062
BseDI CCNNGG 4 cut(s) 490, 885, 978, 1547
BseGI GGATG 2 cut(s) 1161, 1408
BseLI CCNNNNNNNGG 5 cut(s) 449, 519, 833, 1171, 1354
BseMI GCAATG 1 cut(s) 490
BseNI ACTGG 2 cut(s) 64, 838
BseRI GAGGAG 1 cut(s) 1274
BseSI GKGCMC 2 cut(s) 499, 979
BseXI GCAGC 3 cut(s) 242, 251, 1261
BseYI CCCAGC 1 cut(s) 1406
BsgI GTGCAG 1 cut(s) 1327
Bsh1236I CGCG 2 cut(s) 1229, 1361
BshFI GGCC 7 cut(s) 175, 722, 788, 956, 977, 1164, 1367
BshNI GGYRCC 2 cut(s) 187, 494
BshVI ATCGAT 1 cut(s) 1062
BsiHKAI GWGCWC 1 cut(s) 209
BsiHKCI CYCGRG 3 cut(s) 194, 513, 978
BsiSI CCGG 3 cut(s) 450, 979, 1102
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 5 cut(s) 449, 519, 833, 1171, 1354
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 7 cut(s) 175, 722, 788, 956, 977, 1164, 1367
BsoBI CYCGRG 3 cut(s) 194, 513, 978
Bsp120I GGGCCC 1 cut(s) 975
Bsp1286I GDGCHC 3 cut(s) 209, 499, 979
Bsp143I GATC 3 cut(s) 837, 1059, 1063
BspACI CCGC 2 cut(s) 443, 1256
BspANI GGCC 7 cut(s) 175, 722, 788, 956, 977, 1164, 1367
BspDI ATCGAT 1 cut(s) 1062
BspFNI CGCG 2 cut(s) 1229, 1361
BspLI GGNNCC 5 cut(s) 64, 189, 496, 524, 977
BspMAI CTGCAG 1 cut(s) 260
BspOI GCTAGC 1 cut(s) 449
BspPI GGATC 3 cut(s) 845, 1058, 1067
BspT107I GGYRCC 2 cut(s) 187, 494
BsrBI CCGCTC 1 cut(s) 1256
BsrDI GCAATG 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 1101
BsrI ACTGG 2 cut(s) 64, 838
BssAI RCCGGY 1 cut(s) 1101
BssECI CCNNGG 4 cut(s) 490, 885, 978, 1547
BssMI GATC 3 cut(s) 837, 1059, 1063
BssNI GRCGYC 1 cut(s) 1283
BssSI CACGAG 2 cut(s) 359, 732
BssT1I CCWWGG 2 cut(s) 490, 1547
Bst2BI CACGAG 2 cut(s) 359, 732
Bst2UI CCWGG 1 cut(s) 1388
Bst4CI ACNGT 8 cut(s) 716, 763, 889, 1196, 1205, 1485, 1581, 1600
BstACI GRCGYC 1 cut(s) 1283
BstAPI GCANNNNNTGC 1 cut(s) 264
BstC8I GCNNGC 5 cut(s) 177, 316, 447, 1254, 1369
BstDEI CTNAG 3 cut(s) 899, 1461, 1515
BstDSI CCRYGG 1 cut(s) 885
BstF5I GGATG 2 cut(s) 1161, 1408
BstFNI CGCG 2 cut(s) 1229, 1361
BstHHI GCGC 2 cut(s) 125, 1231
BstKTI GATC 3 cut(s) 840, 1062, 1066
BstMBI GATC 3 cut(s) 837, 1059, 1063
BstMWI GCNNNNNNNGC 9 cut(s) 129, 185, 261, 264, 331, 455, 962, 1299, 1511
BstNI CCWGG 1 cut(s) 1388
BstNSI RCATGY 2 cut(s) 1494, 1618
BstSCI CCNGG 3 cut(s) 977, 978, 1386
BstSFI CTRYAG 1 cut(s) 256
BstSLI GKGCMC 2 cut(s) 499, 979
BstUI CGCG 2 cut(s) 1229, 1361
BstV1I GCAGC 3 cut(s) 242, 251, 1261
BstV2I GAAGAC 2 cut(s) 1107, 1458
Bsu15I ATCGAT 1 cut(s) 1062
BsuRI GGCC 7 cut(s) 175, 722, 788, 956, 977, 1164, 1367
BsuTUI ATCGAT 1 cut(s) 1062
BtgI CCRYGG 1 cut(s) 885
BtgZI GCGATG 1 cut(s) 1124
BtrI CACGTC 1 cut(s) 455
BtsCI GGATG 2 cut(s) 1161, 1408
BtsI GCAGTG 1 cut(s) 1070
BtsIMutI CAGTG 5 cut(s) 57, 831, 883, 1070, 1210
Cac8I GCNNGC 5 cut(s) 177, 316, 447, 1254, 1369
CaiI CAGNNNCTG 1 cut(s) 264
CfoI GCGC 2 cut(s) 125, 1231
Cfr10I RCCGGY 1 cut(s) 1101
Cfr13I GGNCC 6 cut(s) 173, 522, 720, 975, 976, 1162
Cfr9I CCCGGG 1 cut(s) 978
ClaI ATCGAT 1 cut(s) 1062
CseI GACGC 3 cut(s) 1235, 1272, 1350
CsiI ACCWGGT 1 cut(s) 1386
Csp6I GTAC 4 cut(s) 26, 759, 1494, 1612
CviAII CATG 8 cut(s) 400, 582, 649, 703, 1399, 1456, 1491, 1615
CviQI GTAC 4 cut(s) 26, 759, 1494, 1612
DdeI CTNAG 3 cut(s) 899, 1461, 1515
DpnI GATC 3 cut(s) 839, 1061, 1065
DpnII GATC 3 cut(s) 837, 1059, 1063
EaeI YGGCCR 2 cut(s) 786, 1365
Eco130I CCWWGG 2 cut(s) 490, 1547
Eco147I AGGCCT 1 cut(s) 956
Eco24I GRGCYC 1 cut(s) 979
Eco47I GGWCC 1 cut(s) 522
Eco88I CYCGRG 3 cut(s) 194, 513, 978
EcoO109I RGGNCCY 2 cut(s) 173, 522
EcoRII CCWGG 1 cut(s) 1386
EcoT14I CCWWGG 2 cut(s) 490, 1547
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 1 cut(s) 979
ErhI CCWWGG 2 cut(s) 490, 1547
FaeI CATG 8 cut(s) 403, 585, 652, 706, 1402, 1459, 1494, 1618
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FaqI GGGAC 1 cut(s) 508
FatI CATG 8 cut(s) 399, 581, 648, 702, 1398, 1455, 1490, 1614
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 256, 265, 1250
FokI GGATG 2 cut(s) 1168, 1415
FriOI GRGCYC 1 cut(s) 979
Fsp4HI GCNGC 3 cut(s) 256, 265, 1250
FspBI CTAG 4 cut(s) 446, 590, 804, 843
GlaI GCGC 2 cut(s) 124, 1230
GluI GCNGC 3 cut(s) 256, 265, 1250
GsaI CCCAGC 1 cut(s) 1410
HaeIII GGCC 7 cut(s) 175, 722, 788, 956, 977, 1164, 1367
HapII CCGG 3 cut(s) 450, 979, 1102
HgaI GACGC 3 cut(s) 1235, 1272, 1350
HhaI GCGC 2 cut(s) 125, 1231
Hin1I GRCGYC 1 cut(s) 1283
Hin1II CATG 8 cut(s) 403, 585, 652, 706, 1402, 1459, 1494, 1618
Hin6I GCGC 2 cut(s) 123, 1229
HinP1I GCGC 2 cut(s) 123, 1229
HincII GTYRAC 2 cut(s) 144, 628
HindII GTYRAC 2 cut(s) 144, 628
HindIII AAGCTT 1 cut(s) 312
HpaI GTTAAC 1 cut(s) 144
HpaII CCGG 3 cut(s) 450, 979, 1102
HphI GGTGA 7 cut(s) 40, 74, 614, 1208, 1286, 1447, 1612
Hpy166II GTNNAC 4 cut(s) 14, 144, 356, 628
Hpy188I TCNGA 2 cut(s) 535, 948
Hpy188III TCNNGA 2 cut(s) 374, 1090
Hpy8I GTNNAC 4 cut(s) 14, 144, 356, 628
Hpy99I CGWCG 8 cut(s) 205, 479, 602, 1126, 1196, 1229, 1288, 1366
HpyAV CCTTC 5 cut(s) 336, 359, 1264, 1382, 1438
HpyCH4III ACNGT 8 cut(s) 716, 763, 889, 1196, 1205, 1485, 1581, 1600
HpyCH4IV ACGT 7 cut(s) 140, 454, 474, 504, 597, 630, 1095
HpyCH4V TGCA 8 cut(s) 149, 258, 267, 483, 666, 689, 744, 1308
HpyF10VI GCNNNNNNNGC 9 cut(s) 129, 185, 261, 264, 331, 455, 962, 1299, 1511
HpyF3I CTNAG 3 cut(s) 899, 1461, 1515
HpySE526I ACGT 7 cut(s) 140, 454, 474, 504, 597, 630, 1095
Hsp92I GRCGYC 1 cut(s) 1283
Hsp92II CATG 8 cut(s) 403, 585, 652, 706, 1402, 1459, 1494, 1618
HspAI GCGC 2 cut(s) 123, 1229
KspAI GTTAAC 1 cut(s) 144
Kzo9I GATC 3 cut(s) 837, 1059, 1063
LmnI GCTCC 2 cut(s) 68, 1341
Lsp1109I GCAGC 3 cut(s) 242, 251, 1261
LweI GCATC 3 cut(s) 676, 1393, 1630
MabI ACCWGGT 1 cut(s) 1386
MaeI CTAG 4 cut(s) 446, 590, 804, 843
MaeII ACGT 7 cut(s) 140, 454, 474, 504, 597, 630, 1095
MaeIII GTNAC 4 cut(s) 602, 856, 1042, 1148
MalI GATC 3 cut(s) 839, 1061, 1065
MbiI CCGCTC 1 cut(s) 1256
MboI GATC 3 cut(s) 837, 1059, 1063
MboII GAAGA 3 cut(s) 304, 1107, 1463
MhlI GDGCHC 3 cut(s) 209, 499, 979
MlsI TGGCCA 1 cut(s) 788
MluCI AATT 8 cut(s) 214, 303, 774, 852, 1438, 1528, 1558, 1669
MluNI TGGCCA 1 cut(s) 788
MmeI TCCRAC 1 cut(s) 90
Mox20I TGGCCA 1 cut(s) 788
Mph1103I ATGCAT 1 cut(s) 691
MscI TGGCCA 1 cut(s) 788
MseI TTAA 7 cut(s) 33, 143, 321, 404, 750, 1380, 1672
MslI CAYNNNNRTG 2 cut(s) 398, 970
Msp20I TGGCCA 1 cut(s) 788
MspI CCGG 3 cut(s) 450, 979, 1102
MspR9I CCNGG 3 cut(s) 979, 980, 1388
MvaI CCWGG 1 cut(s) 1388
MvnI CGCG 2 cut(s) 1229, 1361
MwoI GCNNNNNNNGC 9 cut(s) 129, 185, 261, 264, 331, 455, 962, 1299, 1511
NciI CCSGG 2 cut(s) 979, 980
NdeII GATC 3 cut(s) 837, 1059, 1063
NheI GCTAGC 1 cut(s) 445
NlaIII CATG 8 cut(s) 403, 585, 652, 706, 1402, 1459, 1494, 1618
NlaIV GGNNCC 5 cut(s) 64, 189, 496, 524, 977
NmuCI GTSAC 1 cut(s) 602
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 2 cut(s) 1494, 1618
PaeR7I CTCGAG 1 cut(s) 194
PceI AGGCCT 1 cut(s) 956
PciI ACATGT 1 cut(s) 1614
PflFI GACNNNGTC 1 cut(s) 596
PflMI CCANNNNNTGG 1 cut(s) 833
PkrI GCNGC 3 cut(s) 257, 266, 1251
PpuMI RGGWCCY 1 cut(s) 522
PscI ACATGT 1 cut(s) 1614
Psp1406I AACGTT 1 cut(s) 140
Psp5II RGGWCCY 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 1386
PspFI CCCAGC 1 cut(s) 1406
PspGI CCWGG 1 cut(s) 1386
PspN4I GGNNCC 5 cut(s) 64, 189, 496, 524, 977
PspOMI GGGCCC 1 cut(s) 975
PspPI GGNCC 6 cut(s) 173, 522, 720, 975, 976, 1162
PspPPI RGGWCCY 1 cut(s) 522
PspXI VCTCGAGB 1 cut(s) 194
PstI CTGCAG 1 cut(s) 260
PstNI CAGNNNCTG 1 cut(s) 264
PsyI GACNNNGTC 1 cut(s) 596
RsaI GTAC 4 cut(s) 27, 760, 1495, 1613
RsaNI GTAC 4 cut(s) 26, 759, 1494, 1612
RseI CAYNNNNRTG 2 cut(s) 398, 970
SaqAI TTAA 7 cut(s) 33, 143, 321, 404, 750, 1380, 1672
SatI GCNGC 3 cut(s) 256, 265, 1250
Sau3AI GATC 3 cut(s) 837, 1059, 1063
Sau96I GGNCC 6 cut(s) 173, 522, 720, 975, 976, 1162
ScrFI CCNGG 3 cut(s) 979, 980, 1388
SduI GDGCHC 3 cut(s) 209, 499, 979
SexAI ACCWGGT 1 cut(s) 1386
SfaNI GCATC 3 cut(s) 676, 1393, 1630
SfcI CTRYAG 1 cut(s) 256
Sfr274I CTCGAG 1 cut(s) 194
SinI GGWCC 1 cut(s) 522
SlaI CTCGAG 1 cut(s) 194
SmaI CCCGGG 1 cut(s) 980
SmiMI CAYNNNNRTG 2 cut(s) 398, 970
SmlI CTYRAG 3 cut(s) 157, 194, 996
SmoI CTYRAG 3 cut(s) 157, 194, 996
Sse9I AATT 8 cut(s) 214, 303, 774, 852, 1438, 1528, 1558, 1669
SseBI AGGCCT 1 cut(s) 956
SsiI CCGC 2 cut(s) 443, 1256
SspI AATATT 1 cut(s) 437
SspMI CTAG 4 cut(s) 446, 590, 804, 843
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 3 cut(s) 977, 978, 1386
StyI CCWWGG 2 cut(s) 490, 1547
TaaI ACNGT 8 cut(s) 716, 763, 889, 1196, 1205, 1485, 1581, 1600
TaiI ACGT 7 cut(s) 143, 457, 477, 507, 600, 633, 1098
TaqI TCGA 8 cut(s) 195, 375, 477, 636, 725, 1062, 1191, 1412
TasI AATT 8 cut(s) 214, 303, 774, 852, 1438, 1528, 1558, 1669
TatI WGTACW 3 cut(s) 25, 758, 1493
Tru1I TTAA 7 cut(s) 33, 143, 321, 404, 750, 1380, 1672
Tru9I TTAA 7 cut(s) 33, 143, 321, 404, 750, 1380, 1672
TscAI CASTG 5 cut(s) 64, 838, 883, 1077, 1210
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 3 cut(s) 255, 264, 1249
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 7 cut(s) 17, 388, 688, 1283, 1338, 1387, 1635
TspGWI ACGGA 2 cut(s) 1066, 1343
TspMI CCCGGG 1 cut(s) 978
TspRI CASTG 5 cut(s) 64, 838, 883, 1077, 1210
Tth111I GACNNNGTC 1 cut(s) 596
Van91I CCANNNNNTGG 1 cut(s) 833
VpaK11BI GGWCC 1 cut(s) 522
XapI RAATTY 2 cut(s) 214, 1438
XceI RCATGY 2 cut(s) 1494, 1618
XhoI CTCGAG 1 cut(s) 194
XmaI CCCGGG 1 cut(s) 978
XmiI GTMKAC 1 cut(s) 13
XspI CTAG 4 cut(s) 446, 590, 804, 843
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.