Rroxscaffold_5G00373260

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000852
Physical Location & Seq
Reverse (-)
54547024 .. 54550208
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373260.1

Sequence Viewer

Length: 1287 bp
ATGAACCGGAATCTCAATGTGGGCGAACGGGTGGACGACAAGTCGTTTGAGCACGATGTTGATTGTTTGATCAAGATGAAGGTTGAGGTGATTGAGAGGGATTGCATCAAGCCTTCTTCTTCAACTCCAAACCACCTTCGCAAACATGAGCTTTCATTTCTCGATCAAGTCGCCCCTCCCATTTTCATGCCCATGCTCCTATTCTACCCTGCTACTAATTATGGTCACGGTAATGACGAAAACGCTTTGTCTGCTAGCCGGGTTAAGCAGTCCCTATCCGAGACTTTGACGCAGTTCTATCCGCTAGCCGGACGGATCATCGACAACGCCATTGTCGATTGTAACGATGAAGGTACTCCTTTCGTCGAAGCACGAGTCAACTGCCTACTTTCAGATGTTGTATCCCAACCGGACCCAAACCAACTCAACAAGCTACTCCCATGTGAGTTGGAATGTGTTGTCGACTTTGTTCTTGCAGTCCAAGCCAACATCTTTGACTGTGGAGGCATGGCTATCGGAGTGTGCATTTCGCACAAAATCGCCGACGCCTTGTCACTAGTCATGTTTCTCAACTCTTGGGCTTCCATCTCCCGAGGGGATAATTGTGATTACACAGCTCCCACTTTTGACTTGGCTACGTTCTTCCCGCCGAGATCCATATCCGGGTTCAAACCAGGGACTGGAATCGCAAGAGACAAAATCGTTACTAAACGATTCGTCTTCAGCGCCTCCACCGTCGCTTCTCTCCGAACAAAATACACGACATCTGCAACTGATAGACGCCCGACTCGTATCGAGGCCCTGTCAGCTTTTGTATGGAGCCGTTTCTTGGCTTCCACGCAAGGAAAGCCCAACTCGGACACAGTTTACAAAGTTCTCCACGCAGTCAACCTACGAACCAGAATGGACCCGGCGCTTCCGGAGAGTCATTTCGGAAACGTGAGCCGGTTCGCGACTGCGACGACGTCGTTTGAGGAGGGCGAGGAGGAGTGTTATGGATTTGTGGGACAAATGAGAGATGCCATAAAGACCGTCGACGGGGAGTACATGAGGAAGATTCTAGAAGGCACCGCGCATGTGAATTATTTGAAGCAGCGAGCGGAGGAGTTTCTGAAAGGTGAAGTGGTGTCATTTAGCTTCACCAGTCTCTGTAGGTTTCCATTGTATGAGACTGACTTCGGGTGGGGAAGCCGATATGGGTCGGCTCTGCTAGCCTCACCTTCAAGAATCTGGTTTGTTTCATGGATTCTGGGTCCGGTGGTGGGATCGAAGCGTGGGTTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

428

Amino Acids

47.62

Weight (kDa)

6.11

Isoelectric Point (pI)

33.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 26 - 397 8.1e-76 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 968
AccB1I GGYRCC 1 cut(s) 1067
AccB7I CCANNNNNTGG 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 1100
AccI GTMKAC 2 cut(s) 462, 1035
AccII CGCG 2 cut(s) 953, 1073
AccIII TCCGGA 1 cut(s) 919
AciI CCGC 4 cut(s) 302, 647, 1071, 1100
AclWI GGATC 3 cut(s) 323, 648, 1273
AcuI CTGAAG 1 cut(s) 706
AcyI GRCGYC 3 cut(s) 546, 781, 965
AfaI GTAC 2 cut(s) 355, 1046
AfiI CCNNNNNNNGG 6 cut(s) 308, 663, 680, 829, 1038, 1262
AgsI TTSAA 4 cut(s) 123, 670, 1090, 1224
AhdI GACNNNNNGTC 2 cut(s) 40, 550
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 1 cut(s) 673
AluBI AGCT 5 cut(s) 151, 433, 617, 809, 1137
AluI AGCT 5 cut(s) 151, 433, 617, 809, 1137
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 4 cut(s) 275, 687, 1151, 1163
AlwI GGATC 3 cut(s) 323, 648, 1273
AlwNI CAGNNNCTG 2 cut(s) 680, 1149
Ama87I CYCGRG 1 cut(s) 591
Aor13HI TCCGGA 1 cut(s) 919
AoxI GGCC 1 cut(s) 798
ApeKI GCWGC 1 cut(s) 1093
ArsI GACNNNNNNTTYG 4 cut(s) 29, 61, 360, 392
AspLEI GCGC 3 cut(s) 728, 916, 1075
AspS9I GGNCC 4 cut(s) 412, 799, 907, 1253
AsuC2I CCSGG 3 cut(s) 260, 664, 911
AsuHPI GGTGA 4 cut(s) 100, 1130, 1132, 1209
AsuNHI GCTAGC 3 cut(s) 254, 304, 1210
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 3 cut(s) 412, 907, 1253
BanI GGYRCC 1 cut(s) 1067
BauI CACGAG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 712
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 1105
BccI CCATC 1 cut(s) 593
BceAI ACGGC 1 cut(s) 807
BcgI CGANNNNNNTGC 4 cut(s) 363, 397, 496, 530
BciT130I CCWGG 1 cut(s) 675
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 3 cut(s) 260, 664, 911
BcoDI GTCTC 4 cut(s) 275, 687, 1151, 1163
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 5 cut(s) 255, 305, 557, 1061, 1211
BfmI CTRYAG 1 cut(s) 1150
BfoI RGCGCY 2 cut(s) 729, 917
BfuI GTATCC 1 cut(s) 412
BisI GCNGC 1 cut(s) 1094
BlsI GCNGC 1 cut(s) 1095
Bme1390I CCNGG 4 cut(s) 260, 664, 675, 911
Bme18I GGWCC 3 cut(s) 412, 907, 1253
BmeRI GACNNNNNGTC 2 cut(s) 40, 550
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 4 cut(s) 412, 799, 907, 1253
BmiI GGNNCC 5 cut(s) 414, 821, 909, 1069, 1254
BmrFI CCNGG 4 cut(s) 260, 664, 675, 911
BmsI GCATC 2 cut(s) 114, 1009
BmtI GCTAGC 3 cut(s) 258, 308, 1214
BpiI GAAGAC 1 cut(s) 712
BpuMI CCSGG 3 cut(s) 260, 664, 911
BsaBI GATNNNNATC 1 cut(s) 658
BsaHI GRCGYC 3 cut(s) 546, 781, 965
BsaJI CCNNGG 2 cut(s) 592, 674
BsaWI WCCGGW 4 cut(s) 6, 409, 919, 1255
Bsc4I CCNNNNNNNGG 6 cut(s) 308, 663, 680, 829, 1038, 1262
Bse118I RCCGGY 1 cut(s) 945
Bse1I ACTGG 2 cut(s) 685, 1143
Bse8I GATNNNNATC 1 cut(s) 658
BseAI TCCGGA 1 cut(s) 919
BseBI CCWGG 1 cut(s) 675
BseDI CCNNGG 2 cut(s) 592, 674
BseJI GATNNNNATC 1 cut(s) 658
BseLI CCNNNNNNNGG 6 cut(s) 308, 663, 680, 829, 1038, 1262
BseNI ACTGG 2 cut(s) 685, 1143
BseRI GAGGAG 4 cut(s) 989, 998, 1001, 1118
BseXI GCAGC 1 cut(s) 1105
Bsh1236I CGCG 2 cut(s) 953, 1073
BshFI GGCC 1 cut(s) 800
BshNI GGYRCC 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 54
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 9 cut(s) 7, 259, 309, 410, 663, 911, 920, 946, 1256
BslFI GGGAC 3 cut(s) 256, 691, 1020
BslI CCNNNNNNNGG 6 cut(s) 308, 663, 680, 829, 1038, 1262
BsmAI GTCTC 4 cut(s) 275, 687, 1151, 1163
BsmFI GGGAC 3 cut(s) 256, 691, 1020
BsnI GGCC 1 cut(s) 800
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 1 cut(s) 54
Bsp13I TCCGGA 1 cut(s) 919
Bsp143I GATC 5 cut(s) 69, 163, 315, 653, 1265
Bsp68I TCGCGA 1 cut(s) 953
BspACI CCGC 4 cut(s) 302, 647, 1071, 1100
BspANI GGCC 1 cut(s) 800
BspEI TCCGGA 1 cut(s) 919
BspFNI CGCG 2 cut(s) 953, 1073
BspLI GGNNCC 5 cut(s) 414, 821, 909, 1069, 1254
BspOI GCTAGC 3 cut(s) 258, 308, 1214
BspPI GGATC 3 cut(s) 323, 648, 1273
BspT107I GGYRCC 1 cut(s) 1067
BsrBI CCGCTC 1 cut(s) 1100
BsrFI RCCGGY 1 cut(s) 945
BsrI ACTGG 2 cut(s) 685, 1143
BssAI RCCGGY 1 cut(s) 945
BssECI CCNNGG 2 cut(s) 592, 674
BssMI GATC 5 cut(s) 69, 163, 315, 653, 1265
BssNI GRCGYC 3 cut(s) 546, 781, 965
BssSI CACGAG 1 cut(s) 372
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 5 cut(s) 230, 500, 736, 865, 1033
BstACI GRCGYC 3 cut(s) 546, 781, 965
BstC8I GCNNGC 4 cut(s) 256, 306, 1098, 1212
BstFNI CGCG 2 cut(s) 953, 1073
BstH2I RGCGCY 2 cut(s) 729, 917
BstHHI GCGC 3 cut(s) 728, 916, 1075
BstKTI GATC 5 cut(s) 72, 166, 318, 656, 1268
BstMAI GTCTC 4 cut(s) 275, 687, 1151, 1163
BstMBI GATC 5 cut(s) 69, 163, 315, 653, 1265
BstMWI GCNNNNNNNGC 5 cut(s) 251, 482, 806, 847, 1211
BstNI CCWGG 1 cut(s) 675
BstNSI RCATGY 1 cut(s) 1079
BstSCI CCNGG 4 cut(s) 258, 662, 673, 909
BstSFI CTRYAG 1 cut(s) 1150
BstUI CGCG 2 cut(s) 953, 1073
BstV1I GCAGC 1 cut(s) 1105
BstV2I GAAGAC 1 cut(s) 712
BstX2I RGATCY 1 cut(s) 653
BstYI RGATCY 1 cut(s) 653
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 1 cut(s) 800
BtuMI TCGCGA 1 cut(s) 953
Cac8I GCNNGC 4 cut(s) 256, 306, 1098, 1212
CaiI CAGNNNCTG 2 cut(s) 680, 1149
CfoI GCGC 3 cut(s) 728, 916, 1075
Cfr10I RCCGGY 1 cut(s) 945
Cfr13I GGNCC 4 cut(s) 412, 799, 907, 1253
CseI GACGC 3 cut(s) 298, 554, 789
Csp6I GTAC 2 cut(s) 354, 1045
CviAII CATG 9 cut(s) 146, 187, 193, 441, 508, 562, 1048, 1076, 1242
CviQI GTAC 2 cut(s) 354, 1045
DpnI GATC 5 cut(s) 71, 165, 317, 655, 1267
DpnII GATC 5 cut(s) 69, 163, 315, 653, 1265
DriI GACNNNNNGTC 2 cut(s) 40, 550
Eam1105I GACNNNNNGTC 2 cut(s) 40, 550
Eco47I GGWCC 3 cut(s) 412, 907, 1253
Eco57I CTGAAG 1 cut(s) 706
Eco88I CYCGRG 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 799
EcoRII CCWGG 1 cut(s) 673
FaeI CATG 9 cut(s) 149, 190, 196, 444, 511, 565, 1051, 1079, 1245
FaqI GGGAC 3 cut(s) 256, 691, 1020
FatI CATG 9 cut(s) 145, 186, 192, 440, 507, 561, 1047, 1075, 1241
FauI CCCGC 1 cut(s) 654
FbaI TGATCA 1 cut(s) 69
FblI GTMKAC 2 cut(s) 462, 1035
Fnu4HI GCNGC 1 cut(s) 1094
Fsp4HI GCNGC 1 cut(s) 1094
FspBI CTAG 5 cut(s) 255, 305, 557, 1061, 1211
GlaI GCGC 3 cut(s) 727, 915, 1074
GluI GCNGC 1 cut(s) 1094
HaeII RGCGCY 2 cut(s) 729, 917
HaeIII GGCC 1 cut(s) 800
HapII CCGG 9 cut(s) 7, 259, 309, 410, 663, 911, 920, 946, 1256
HgaI GACGC 3 cut(s) 298, 554, 789
HhaI GCGC 3 cut(s) 728, 916, 1075
Hin1I GRCGYC 3 cut(s) 546, 781, 965
Hin1II CATG 9 cut(s) 149, 190, 196, 444, 511, 565, 1051, 1079, 1245
Hin6I GCGC 3 cut(s) 726, 914, 1073
HinP1I GCGC 3 cut(s) 726, 914, 1073
HincII GTYRAC 4 cut(s) 379, 463, 889, 1036
HindII GTYRAC 4 cut(s) 379, 463, 889, 1036
HinfI GANTC 9 cut(s) 10, 375, 684, 714, 787, 925, 1057, 1227, 1246
HpaII CCGG 9 cut(s) 7, 259, 309, 410, 663, 911, 920, 946, 1256
HphI GGTGA 4 cut(s) 100, 1130, 1132, 1209
Hpy166II GTNNAC 6 cut(s) 34, 379, 463, 868, 889, 1036
Hpy188I TCNGA 7 cut(s) 280, 394, 518, 749, 859, 935, 1113
Hpy188III TCNNGA 7 cut(s) 73, 161, 591, 920, 952, 1061, 1224
Hpy8I GTNNAC 6 cut(s) 34, 379, 463, 868, 889, 1036
Hpy99I CGWCG 8 cut(s) 368, 548, 740, 964, 967, 970, 1037, 1040
HpyAV CCTTC 6 cut(s) 73, 123, 146, 344, 1058, 1230
HpyCH4III ACNGT 5 cut(s) 230, 500, 736, 865, 1033
HpyCH4IV ACGT 3 cut(s) 638, 939, 965
HpyCH4V TGCA 4 cut(s) 105, 476, 525, 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 251, 482, 806, 847, 1211
HpySE526I ACGT 3 cut(s) 638, 939, 965
Hsp92I GRCGYC 3 cut(s) 546, 781, 965
Hsp92II CATG 9 cut(s) 149, 190, 196, 444, 511, 565, 1051, 1079, 1245
HspAI GCGC 3 cut(s) 726, 914, 1073
Kpn2I TCCGGA 1 cut(s) 919
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 5 cut(s) 69, 163, 315, 653, 1265
LmnI GCTCC 3 cut(s) 201, 622, 819
Lsp1109I GCAGC 1 cut(s) 1105
LweI GCATC 2 cut(s) 114, 1009
MaeI CTAG 5 cut(s) 255, 305, 557, 1061, 1211
MaeII ACGT 3 cut(s) 638, 939, 965
MaeIII GTNAC 4 cut(s) 224, 341, 552, 703
MalI GATC 5 cut(s) 71, 165, 317, 655, 1267
MbiI CCGCTC 1 cut(s) 1100
MboI GATC 5 cut(s) 69, 163, 315, 653, 1265
MboII GAAGA 5 cut(s) 108, 111, 634, 712, 1066
MflI RGATCY 1 cut(s) 653
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 4 cut(s) 217, 601, 1081, 1281
MlyI GAGTC 3 cut(s) 384, 781, 934
MmeI TCCRAC 1 cut(s) 429
MroI TCCGGA 1 cut(s) 919
MseI TTAA 2 cut(s) 264, 1280
MslI CAYNNNNRTG 3 cut(s) 185, 191, 231
MspI CCGG 9 cut(s) 7, 259, 309, 410, 663, 911, 920, 946, 1256
MspR9I CCNGG 4 cut(s) 260, 664, 675, 911
MvaI CCWGG 1 cut(s) 675
MvnI CGCG 2 cut(s) 953, 1073
MwoI GCNNNNNNNGC 5 cut(s) 251, 482, 806, 847, 1211
NciI CCSGG 3 cut(s) 260, 664, 911
NdeII GATC 5 cut(s) 69, 163, 315, 653, 1265
NheI GCTAGC 3 cut(s) 254, 304, 1210
NlaIII CATG 9 cut(s) 149, 190, 196, 444, 511, 565, 1051, 1079, 1245
NlaIV GGNNCC 5 cut(s) 414, 821, 909, 1069, 1254
NmeAIII GCCGAG 1 cut(s) 675
NmuCI GTSAC 2 cut(s) 224, 552
NruI TCGCGA 1 cut(s) 953
NspI RCATGY 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 4 cut(s) 234, 333, 342, 787
PfeI GAWTC 6 cut(s) 10, 684, 714, 1057, 1227, 1246
PflFI GACNNNGTC 1 cut(s) 964
PflMI CCANNNNNTGG 1 cut(s) 680
PkrI GCNGC 1 cut(s) 1095
PleI GAGTC 3 cut(s) 383, 781, 933
PpsI GAGTC 3 cut(s) 383, 781, 933
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 5 cut(s) 414, 821, 909, 1069, 1254
PspPI GGNCC 4 cut(s) 412, 799, 907, 1253
PstNI CAGNNNCTG 2 cut(s) 680, 1149
PsuI RGATCY 1 cut(s) 653
PsyI GACNNNGTC 1 cut(s) 964
RruI TCGCGA 1 cut(s) 953
RsaI GTAC 2 cut(s) 355, 1046
RsaNI GTAC 2 cut(s) 354, 1045
RseI CAYNNNNRTG 3 cut(s) 185, 191, 231
SalI GTCGAC 2 cut(s) 461, 1034
SaqAI TTAA 2 cut(s) 264, 1280
SatI GCNGC 1 cut(s) 1094
Sau3AI GATC 5 cut(s) 69, 163, 315, 653, 1265
Sau96I GGNCC 4 cut(s) 412, 799, 907, 1253
SchI GAGTC 3 cut(s) 384, 781, 934
ScrFI CCNGG 4 cut(s) 260, 664, 675, 911
SduI GDGCHC 1 cut(s) 54
SfaNI GCATC 2 cut(s) 114, 1009
SfcI CTRYAG 1 cut(s) 1150
SgrDI CGTCGACG 1 cut(s) 1034
SinI GGWCC 3 cut(s) 412, 907, 1253
SmiMI CAYNNNNRTG 3 cut(s) 185, 191, 231
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 4 cut(s) 217, 601, 1081, 1281
SsiI CCGC 4 cut(s) 302, 647, 1071, 1100
SspMI CTAG 5 cut(s) 255, 305, 557, 1061, 1211
StyD4I CCNGG 4 cut(s) 258, 662, 673, 909
TaaI ACNGT 5 cut(s) 230, 500, 736, 865, 1033
TaiI ACGT 3 cut(s) 641, 942, 968
TaqI TCGA 8 cut(s) 162, 321, 336, 366, 462, 795, 1035, 1268
TasI AATT 4 cut(s) 217, 601, 1081, 1281
TatI WGTACW 1 cut(s) 1044
TfiI GAWTC 6 cut(s) 10, 684, 714, 1057, 1227, 1246
Tru1I TTAA 2 cut(s) 264, 1280
Tru9I TTAA 2 cut(s) 264, 1280
TseFI GTSAC 2 cut(s) 224, 552
TseI GCWGC 1 cut(s) 1093
Tsp45I GTSAC 2 cut(s) 224, 552
TspDTI ATGAA 6 cut(s) 17, 92, 144, 175, 363, 1230
TspGWI ACGGA 1 cut(s) 328
Tth111I GACNNNGTC 1 cut(s) 964
Van91I CCANNNNNTGG 1 cut(s) 680
VpaK11BI GGWCC 3 cut(s) 412, 907, 1253
XbaI TCTAGA 1 cut(s) 1060
XceI RCATGY 1 cut(s) 1079
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XmiI GTMKAC 2 cut(s) 462, 1035
XspI CTAG 5 cut(s) 255, 305, 557, 1061, 1211
ZraI GACGTC 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.