MD13G1109000.v1.1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000852
Physical Location & Seq
Forward (+)
7881901 .. 7883196
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1109000.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGAAGATTGAAGTAATTGAGAAAGCTTGCATTAAGCCTTCTATGCCAACCCCACCCCACCTTCGTAAACACGAGTTTTCTTTTCTCGACCAAATCGCCACTCCCATTTTCATGCCTATGATCCTTTTCTACAATAATGATCATGGCAATGACGCCATAACATACTCAGACAAGGTAAAACAATCCTTATCTAAGACCCTGACACAATATTATCCATTGGCTGGACGTGTCATTGAAAATGCCTACGTCAATTGCAATGACCAAGGTGCCCCTTACGTCCAAGCCCGAGTTAGGTCCCCGCTTTCTGATGTCATATCCCAACCCGACCCAAACCAACTCAACCAACTACTCCCATGCAAACTAGACAATGTCGGTGACTTGGTTCTTGCTGTCCAAGTCAACGTGTTCGATTGTGGTGGCATGGCTATTGGAATTTGCATTTCTCACAAAGTTGCTGATGCATTGTCACTCATCATGTTCCTCAACAGTTGGGCATCGATATCTCGTGGGGATTGCAACATTAAAAATAGTACAGTCAGTCCTCCAATTTTTGATTTGGCCACGCACTTTCCACCTAGAAGCATATCAGGGTTCAAACCAAGCACTGGGATCACTAGAGAAAAAATTGTTACAAAAAGGTTTGTTTTCAGTGCTTCCACGGTTGCTTCTCTTAGGTCAAAATATACAGATAATAAAACCAATGAGAGAAAGCCAACTCGGATTGAGGCCTTGTCAGCATTTATATGGGCCCGGGTTATTGCCTCCACTCAAGGAAAGCCGAACCCTAACACTCTTTACAAAGTGCTTCACGCCGTTAACTTACGGACGAGGATGGATCCACCACTGCCCGAATACCATTTCGGGAACGTGAGCCGGTTTGCGATGTCATCGCCGTCGTTTGATGAGGGAGAGGATATGTGTTACGGGATGGTGGGCCAGATGAGGGAAGCAATAAAGACAATCGATGGTGATTACATGGCCAAAATCAAAGACGGCAACGCGCACTTGAAGTTTTTGAAGCAGCGAGCGGAGGAGTTTATGAAGGGTGAAGTGGCGTCGTTTAGCTTTACCAGCTTGTGCAGGTTCCCACTTTATGAAACGGACTTCGGGTGGGGCAAGCCAGTGTGGGTCGCGTCGGCCAGCCTTACATTTAAGAACCTGGTTGTTTTCATGGATGCTGGGTCGAGTGGTGGGATTGAAGTGTGGGTGAATTTGAAGGAGGAAGACATGGCTAAGTTCCAAGATGATGAGCAACTGCTGGCATGTAGTTATCCAGCCCAAGAGGCTAAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

432

Amino Acids

48.09

Weight (kDa)

7.98

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 418 6.3e-88 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1071
AccB1I GGYRCC 1 cut(s) 266
AccB7I CCANNNNNTGG 2 cut(s) 221, 605
AccBSI CCGCTC 1 cut(s) 1028
AccII CGCG 2 cut(s) 1001, 1133
AciI CCGC 2 cut(s) 299, 1028
AclWI GGATC 4 cut(s) 115, 617, 830, 843
AcoI YGGCCR 3 cut(s) 558, 978, 1137
AcsI RAATTY 2 cut(s) 432, 1210
AcyI GRCGYC 2 cut(s) 153, 1055
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 4 cut(s) 221, 291, 605, 943
AflIII ACRYGT 2 cut(s) 226, 402
AgsI TTSAA 7 cut(s) 11, 236, 595, 1009, 1018, 1199, 1216
AjiI CACGTC 1 cut(s) 227
AjnI CCWGG 1 cut(s) 1158
AluBI AGCT 3 cut(s) 26, 1065, 1074
AluI AGCT 3 cut(s) 26, 1065, 1074
AlwI GGATC 4 cut(s) 115, 617, 830, 843
Ama87I CYCGRG 2 cut(s) 285, 750
AoxI GGCC 6 cut(s) 558, 726, 747, 934, 978, 1137
ApaI GGGCCC 1 cut(s) 751
ApeKI GCWGC 1 cut(s) 1021
ApoI RAATTY 2 cut(s) 432, 1210
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 4 cut(s) 294, 747, 748, 934
AsuC2I CCSGG 2 cut(s) 751, 752
AsuHPI GGTGA 4 cut(s) 386, 980, 1058, 1219
AvaI CYCGRG 2 cut(s) 285, 750
AvaII GGWCC 1 cut(s) 294
BaeGI GKGCMC 2 cut(s) 271, 751
BalI TGGCCA 2 cut(s) 560, 980
BamHI GGATCC 1 cut(s) 835
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 751
BauI CACGAG 2 cut(s) 71, 504
BbsI GAAGAC 1 cut(s) 1230
BbvI GCAGC 1 cut(s) 1033
BccI CCATC 3 cut(s) 826, 922, 959
BceAI ACGGC 3 cut(s) 797, 877, 1009
BciT130I CCWGG 1 cut(s) 1160
BclI TGATCA 1 cut(s) 139
BcnI CCSGG 2 cut(s) 751, 752
BfaI CTAG 3 cut(s) 362, 576, 615
BfuAI ACCTGC 1 cut(s) 1071
BglI GCCNNNNNGGC 1 cut(s) 1283
BisI GCNGC 1 cut(s) 1022
BlsI GCNGC 1 cut(s) 1023
Bme1390I CCNGG 3 cut(s) 751, 752, 1160
Bme18I GGWCC 1 cut(s) 294
BmeT110I CYCGRG 2 cut(s) 285, 750
BmgBI CACGTC 1 cut(s) 227
BmgT120I GGNCC 4 cut(s) 294, 747, 748, 934
BmiI GGNNCC 5 cut(s) 268, 296, 749, 837, 1085
BmrFI CCNGG 3 cut(s) 751, 752, 1160
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 3 cut(s) 448, 503, 1165
BmuI ACTGGG 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 1230
Bpu10I CCTNAGC 1 cut(s) 1287
BpuEI CTTGAG 1 cut(s) 753
BpuMI CCSGG 2 cut(s) 751, 752
Bsa29I ATCGAT 2 cut(s) 497, 963
BsaHI GRCGYC 2 cut(s) 153, 1055
BsaJI CCNNGG 3 cut(s) 262, 657, 750
Bsc4I CCNNNNNNNGG 4 cut(s) 221, 291, 605, 943
Bse118I RCCGGY 1 cut(s) 873
Bse1I ACTGG 2 cut(s) 610, 1121
Bse3DI GCAATG 2 cut(s) 154, 262
BseBI CCWGG 1 cut(s) 1160
BseCI ATCGAT 2 cut(s) 497, 963
BseDI CCNNGG 3 cut(s) 262, 657, 750
BseGI GGATG 3 cut(s) 837, 933, 1180
BseLI CCNNNNNNNGG 4 cut(s) 221, 291, 605, 943
BseMI GCAATG 2 cut(s) 154, 262
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 610, 1121
BseRI GAGGAG 1 cut(s) 1046
BseSI GKGCMC 2 cut(s) 271, 751
BseXI GCAGC 1 cut(s) 1033
BseYI CCCAGC 1 cut(s) 1178
BsgI GTGCAG 1 cut(s) 1099
Bsh1236I CGCG 2 cut(s) 1001, 1133
BshFI GGCC 6 cut(s) 560, 728, 749, 936, 980, 1139
BshNI GGYRCC 1 cut(s) 266
BshVI ATCGAT 2 cut(s) 497, 963
BsiHKCI CYCGRG 2 cut(s) 285, 750
BsiSI CCGG 2 cut(s) 751, 874
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 4 cut(s) 221, 291, 605, 943
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 6 cut(s) 560, 728, 749, 936, 980, 1139
BsoBI CYCGRG 2 cut(s) 285, 750
Bsp120I GGGCCC 1 cut(s) 747
Bsp1286I GDGCHC 2 cut(s) 271, 751
Bsp143I GATC 4 cut(s) 120, 139, 609, 835
BspACI CCGC 2 cut(s) 299, 1028
BspANI GGCC 6 cut(s) 560, 728, 749, 936, 980, 1139
BspCNI CTCAG 1 cut(s) 179
BspDI ATCGAT 2 cut(s) 497, 963
BspFNI CGCG 2 cut(s) 1001, 1133
BspLI GGNNCC 5 cut(s) 268, 296, 749, 837, 1085
BspMI ACCTGC 1 cut(s) 1071
BspPI GGATC 4 cut(s) 115, 617, 830, 843
BspT107I GGYRCC 1 cut(s) 266
BsrBI CCGCTC 1 cut(s) 1028
BsrDI GCAATG 2 cut(s) 154, 262
BsrFI RCCGGY 1 cut(s) 873
BsrI ACTGG 2 cut(s) 610, 1121
BssAI RCCGGY 1 cut(s) 873
BssECI CCNNGG 3 cut(s) 262, 657, 750
BssMI GATC 4 cut(s) 120, 139, 609, 835
BssNI GRCGYC 2 cut(s) 153, 1055
BssSI CACGAG 2 cut(s) 71, 504
BssT1I CCWWGG 1 cut(s) 262
Bst2BI CACGAG 2 cut(s) 71, 504
Bst2UI CCWGG 1 cut(s) 1160
Bst4CI ACNGT 3 cut(s) 488, 535, 661
BstACI GRCGYC 2 cut(s) 153, 1055
BstC8I GCNNGC 5 cut(s) 28, 1026, 1118, 1141, 1260
BstDEI CTNAG 5 cut(s) 166, 192, 671, 1233, 1287
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 3 cut(s) 837, 933, 1180
BstFNI CGCG 2 cut(s) 1001, 1133
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 4 cut(s) 123, 142, 612, 838
BstMBI GATC 4 cut(s) 120, 139, 609, 835
BstMWI GCNNNNNNNGC 4 cut(s) 43, 734, 1071, 1283
BstNI CCWGG 1 cut(s) 1160
BstNSI RCATGY 1 cut(s) 1266
BstSCI CCNGG 3 cut(s) 749, 750, 1158
BstSLI GKGCMC 2 cut(s) 271, 751
BstUI CGCG 2 cut(s) 1001, 1133
BstV1I GCAGC 1 cut(s) 1033
BstV2I GAAGAC 1 cut(s) 1230
BstX2I RGATCY 1 cut(s) 835
BstYI RGATCY 1 cut(s) 835
Bsu15I ATCGAT 2 cut(s) 497, 963
BsuRI GGCC 6 cut(s) 560, 728, 749, 936, 980, 1139
BsuTUI ATCGAT 2 cut(s) 497, 963
BtgI CCRYGG 1 cut(s) 657
BtgZI GCGATG 2 cut(s) 873, 896
BtrI CACGTC 1 cut(s) 227
BtsCI GGATG 3 cut(s) 837, 933, 1180
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 4 cut(s) 603, 655, 842, 1128
BveI ACCTGC 1 cut(s) 1071
Cac8I GCNNGC 5 cut(s) 28, 1026, 1118, 1141, 1260
CfoI GCGC 1 cut(s) 1003
Cfr10I RCCGGY 1 cut(s) 873
Cfr13I GGNCC 4 cut(s) 294, 747, 748, 934
Cfr9I CCCGGG 1 cut(s) 750
ClaI ATCGAT 2 cut(s) 497, 963
CseI GACGC 3 cut(s) 161, 1044, 1122
CsiI ACCWGGT 1 cut(s) 1158
Csp6I GTAC 1 cut(s) 531
CviAII CATG 9 cut(s) 112, 143, 354, 421, 475, 976, 1171, 1228, 1263
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 5 cut(s) 166, 192, 671, 1233, 1287
DpnI GATC 4 cut(s) 122, 141, 611, 837
DpnII GATC 4 cut(s) 120, 139, 609, 835
EaeI YGGCCR 3 cut(s) 558, 978, 1137
Eco130I CCWWGG 1 cut(s) 262
Eco147I AGGCCT 1 cut(s) 728
Eco24I GRGCYC 1 cut(s) 751
Eco32I GATATC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 294
Eco88I CYCGRG 2 cut(s) 285, 750
EcoO109I RGGNCCY 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 1158
EcoRV GATATC 1 cut(s) 501
EcoT14I CCWWGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 751
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 9 cut(s) 115, 146, 357, 424, 478, 979, 1174, 1231, 1266
FaqI GGGAC 1 cut(s) 280
FatI CATG 9 cut(s) 111, 142, 353, 420, 474, 975, 1170, 1227, 1262
FauI CCCGC 1 cut(s) 306
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 1022
FokI GGATG 3 cut(s) 844, 940, 1187
FriOI GRGCYC 1 cut(s) 751
Fsp4HI GCNGC 1 cut(s) 1022
FspBI CTAG 3 cut(s) 362, 576, 615
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 1 cut(s) 1022
GsaI CCCAGC 1 cut(s) 1182
HaeIII GGCC 6 cut(s) 560, 728, 749, 936, 980, 1139
HapII CCGG 2 cut(s) 751, 874
HgaI GACGC 3 cut(s) 161, 1044, 1122
HhaI GCGC 1 cut(s) 1003
Hin1I GRCGYC 2 cut(s) 153, 1055
Hin1II CATG 9 cut(s) 115, 146, 357, 424, 478, 979, 1174, 1231, 1266
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HincII GTYRAC 2 cut(s) 400, 817
HindII GTYRAC 2 cut(s) 400, 817
HindIII AAGCTT 1 cut(s) 24
HpaI GTTAAC 1 cut(s) 817
HpaII CCGG 2 cut(s) 751, 874
HphI GGTGA 4 cut(s) 386, 980, 1058, 1219
Hpy166II GTNNAC 3 cut(s) 68, 400, 817
Hpy188I TCNGA 3 cut(s) 169, 307, 720
Hpy188III TCNNGA 2 cut(s) 86, 862
Hpy8I GTNNAC 3 cut(s) 68, 400, 817
Hpy99I CGWCG 3 cut(s) 898, 1060, 1138
HpyAV CCTTC 4 cut(s) 48, 71, 1036, 1210
HpyCH4III ACNGT 3 cut(s) 488, 535, 661
HpyCH4IV ACGT 5 cut(s) 226, 246, 276, 402, 867
HpyCH4V TGCA 7 cut(s) 30, 255, 357, 438, 461, 516, 1080
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 734, 1071, 1283
HpyF3I CTNAG 5 cut(s) 166, 192, 671, 1233, 1287
HpySE526I ACGT 5 cut(s) 226, 246, 276, 402, 867
Hsp92I GRCGYC 2 cut(s) 153, 1055
Hsp92II CATG 9 cut(s) 115, 146, 357, 424, 478, 979, 1174, 1231, 1266
HspAI GCGC 1 cut(s) 1001
Ksp22I TGATCA 1 cut(s) 139
KspAI GTTAAC 1 cut(s) 817
Kzo9I GATC 4 cut(s) 120, 139, 609, 835
Lsp1109I GCAGC 1 cut(s) 1033
LweI GCATC 3 cut(s) 448, 503, 1165
MabI ACCWGGT 1 cut(s) 1158
MaeI CTAG 3 cut(s) 362, 576, 615
MaeII ACGT 5 cut(s) 226, 246, 276, 402, 867
MaeIII GTNAC 4 cut(s) 374, 465, 628, 920
MalI GATC 4 cut(s) 122, 141, 611, 837
MbiI CCGCTC 1 cut(s) 1028
MboI GATC 4 cut(s) 120, 139, 609, 835
MboII GAAGA 2 cut(s) 16, 1235
MfeI CAATTG 1 cut(s) 250
MflI RGATCY 1 cut(s) 835
MhlI GDGCHC 2 cut(s) 271, 751
MlsI TGGCCA 2 cut(s) 560, 980
MluCI AATT 6 cut(s) 15, 250, 432, 546, 624, 1210
MluNI TGGCCA 2 cut(s) 560, 980
Mox20I TGGCCA 2 cut(s) 560, 980
Mph1103I ATGCAT 1 cut(s) 463
MscI TGGCCA 2 cut(s) 560, 980
MseI TTAA 4 cut(s) 33, 522, 816, 1152
MslI CAYNNNNRTG 4 cut(s) 110, 116, 147, 742
Msp20I TGGCCA 2 cut(s) 560, 980
MspI CCGG 2 cut(s) 751, 874
MspR9I CCNGG 3 cut(s) 751, 752, 1160
MunI CAATTG 1 cut(s) 250
MvaI CCWGG 1 cut(s) 1160
MvnI CGCG 2 cut(s) 1001, 1133
MwoI GCNNNNNNNGC 4 cut(s) 43, 734, 1071, 1283
NciI CCSGG 2 cut(s) 751, 752
NdeII GATC 4 cut(s) 120, 139, 609, 835
NlaIII CATG 9 cut(s) 115, 146, 357, 424, 478, 979, 1174, 1231, 1266
NlaIV GGNNCC 5 cut(s) 268, 296, 749, 837, 1085
NmuCI GTSAC 2 cut(s) 374, 465
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 1 cut(s) 1266
PceI AGGCCT 1 cut(s) 728
PflFI GACNNNGTC 1 cut(s) 368
PflMI CCANNNNNTGG 2 cut(s) 221, 605
PkrI GCNGC 1 cut(s) 1023
PpuMI RGGWCCY 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 1158
PspFI CCCAGC 1 cut(s) 1178
PspGI CCWGG 1 cut(s) 1158
PspN4I GGNNCC 5 cut(s) 268, 296, 749, 837, 1085
PspOMI GGGCCC 1 cut(s) 747
PspPI GGNCC 4 cut(s) 294, 747, 748, 934
PspPPI RGGWCCY 1 cut(s) 294
PsuI RGATCY 1 cut(s) 835
PsyI GACNNNGTC 1 cut(s) 368
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
RseI CAYNNNNRTG 4 cut(s) 110, 116, 147, 742
SaqAI TTAA 4 cut(s) 33, 522, 816, 1152
SatI GCNGC 1 cut(s) 1022
Sau3AI GATC 4 cut(s) 120, 139, 609, 835
Sau96I GGNCC 4 cut(s) 294, 747, 748, 934
ScrFI CCNGG 3 cut(s) 751, 752, 1160
SduI GDGCHC 2 cut(s) 271, 751
SexAI ACCWGGT 1 cut(s) 1158
SfaNI GCATC 3 cut(s) 448, 503, 1165
SinI GGWCC 1 cut(s) 294
SmaI CCCGGG 1 cut(s) 752
SmiMI CAYNNNNRTG 4 cut(s) 110, 116, 147, 742
SmlI CTYRAG 1 cut(s) 768
SmoI CTYRAG 1 cut(s) 768
Sse9I AATT 6 cut(s) 15, 250, 432, 546, 624, 1210
SseBI AGGCCT 1 cut(s) 728
SsiI CCGC 2 cut(s) 299, 1028
SspI AATATT 1 cut(s) 209
SspMI CTAG 3 cut(s) 362, 576, 615
StuI AGGCCT 1 cut(s) 728
StyD4I CCNGG 3 cut(s) 749, 750, 1158
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 3 cut(s) 488, 535, 661
TaiI ACGT 5 cut(s) 229, 249, 279, 405, 870
TaqI TCGA 5 cut(s) 87, 408, 497, 963, 1184
TasI AATT 6 cut(s) 15, 250, 432, 546, 624, 1210
TatI WGTACW 1 cut(s) 530
Tru1I TTAA 4 cut(s) 33, 522, 816, 1152
Tru9I TTAA 4 cut(s) 33, 522, 816, 1152
TscAI CASTG 4 cut(s) 610, 655, 849, 1128
TseFI GTSAC 2 cut(s) 374, 465
TseI GCWGC 1 cut(s) 1021
Tsp45I GTSAC 2 cut(s) 374, 465
TspDTI ATGAA 5 cut(s) 17, 100, 1055, 1110, 1159
TspGWI ACGGA 2 cut(s) 838, 1115
TspMI CCCGGG 1 cut(s) 750
TspRI CASTG 4 cut(s) 610, 655, 849, 1128
Tth111I GACNNNGTC 1 cut(s) 368
Van91I CCANNNNNTGG 2 cut(s) 221, 605
VpaK11BI GGWCC 1 cut(s) 294
XapI RAATTY 2 cut(s) 432, 1210
XceI RCATGY 1 cut(s) 1266
XmaI CCCGGG 1 cut(s) 750
XspI CTAG 3 cut(s) 362, 576, 615
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.