Rw1G011120

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000852
Physical Location & Seq
Forward (+)
25103311 .. 25104958
1648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011120.1

Sequence Viewer

Length: 1344 bp
ATGAAGTTTCAAGTGGAGGTTCTCTCAACAGAGACAATCAAACCCTCATCTCCAACCCCTGACCACCTCCGCCACCACAAGCTCTCCTACCTCGACCAAATCCAGCCCCCAATCTTCATGCCCATGGTCCTCTTTTACCCGAAAGAACCCGACCACAACGAACTGGACCTCATTTCCATCCACGAACAGCGTTGCAGCAAAATCAAGAAAGCCTTATCCGACACCTTAGTCAAGTTCTACCCACTAGCGGGAAGAGTCCACGGAACCCAATACGTCGATTGCAACGACGAAGGAGCCTACTACGTCGAAGCCAAGACCGACTGCAAAATCTCTGACATCATCGACAACCCAAACCCTAAGGACTTCCACAAGTTCCTCCCTTTCGAGCTAGACGCAGCTCATGAGCTTCCCGTGTGCTTCCAGGTCACCTTCTTCGCCTGTGGCGGCATGAGCATCGGCACGGGGATGTCCCACAAGGTCGGGGACGCCCTCTCCTACATCACGTTCCTCAATGGCTTGGCCGCTGAGGCTCGTGGGGAGGGCAACGATATTCACCCTCCCGAGTTCGTCTCAGACAAGTACTTCCCCCAAAAGGACTTGTCCGGATTCTACCAACACCGCACCGGGATCATAAAGCAAAACATCTCAACAAAACGCTTGGTCTTCGACGCTCCAATGGTTCAGGCCATCCAAGTCAAATGCATGGACATGTCCAGACGCCCCACTCGTGTTGAAGCCCTATCAGCCTTCATATGGAGCCGCTACATAACATCATGCACCGGCATTCAACAAGATGGCGTCACCACCGACGCGATGTACACTGTTTCTCATGCTGTGAACCTCCGCACGAGGATGGAGCCACCACTTCCCGAGTACACATTCGGAAACGTGATCCAAACCACCATTGCGGTGCCGCACATACCCGTTGATCCGAAAGATGGGACGCTTCATGGGGTGGTTAATCACGTCAGGGAGGCCATTAAGCAAGTAGACAAAGAGTACATCAAGAAATTGCAAGAGGGTGACGGACACTTGAGCTTCTTGAGGAGCCGTGCGGAGGAGGTGAAACGCGGAGAGGTGGTGTCGTTTAACTTCACGAGTCTGTGTAGGTTTCCGATCTACGACGCTGATTTTGGATGGGGGAAGCCCGTTTATGTTGGTTCTGCCAGCTTTACGTTCAAGAACTTGGTTAGTTTCTTTGACACCAACGTAGGGGACGGAGTTGAGGCGTGGATCAATCTGTTGGAGGAAGACATGGAAAAATTCGAGGCGGACGAAGAGCTTCTCAAGTATGTTTCACCCGCCCCGAGTTCAAAGAATGCCAAGGCCTCGATTAATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

447

Amino Acids

50.43

Weight (kDa)

5.82

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 428 5.2e-80 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 910
AccI GTMKAC 1 cut(s) 990
AccII CGCG 2 cut(s) 812, 1071
AccIII TCCGGA 1 cut(s) 602
AclWI GGATC 4 cut(s) 635, 886, 923, 1241
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 1 cut(s) 1262
AcyI GRCGYC 3 cut(s) 486, 718, 798
AfaI GTAC 4 cut(s) 581, 818, 875, 1001
AfiI CCNNNNNNNGG 7 cut(s) 247, 248, 592, 753, 938, 1057, 1212
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 5 cut(s) 11, 734, 788, 1180, 1314
AjiI CACGTC 1 cut(s) 967
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 7 cut(s) 82, 388, 398, 406, 1038, 1170, 1282
AluI AGCT 7 cut(s) 82, 388, 398, 406, 1038, 1170, 1282
Alw26I GTCTC 2 cut(s) 26, 574
AlwI GGATC 4 cut(s) 635, 886, 923, 1241
Ama87I CYCGRG 3 cut(s) 560, 869, 1306
Aor13HI TCCGGA 1 cut(s) 602
AoxI GGCC 4 cut(s) 519, 684, 975, 1326
ApeKI GCWGC 2 cut(s) 195, 395
ApoI RAATTY 1 cut(s) 1262
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
AseI ATTAAT 1 cut(s) 1335
Asp700I GAANNNNTTC 1 cut(s) 1281
AspS9I GGNCC 2 cut(s) 127, 166
AsuC2I CCSGG 1 cut(s) 625
AsuHPI GGTGA 6 cut(s) 418, 545, 793, 1034, 1075, 1290
AvaI CYCGRG 3 cut(s) 560, 869, 1306
AvaII GGWCC 2 cut(s) 127, 166
AxyI CCTNAGG 1 cut(s) 357
BanI GGYRCC 1 cut(s) 910
BauI CACGAG 4 cut(s) 531, 726, 847, 1096
BbsI GAAGAC 2 cut(s) 655, 1257
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 207, 407
BccI CCATC 6 cut(s) 185, 695, 788, 847, 932, 1131
BceAI ACGGC 1 cut(s) 1035
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 1 cut(s) 422
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 26, 574
BfaI CTAG 2 cut(s) 245, 389
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 6 cut(s) 196, 396, 445, 522, 760, 914
BlsI GCNGC 6 cut(s) 197, 397, 446, 523, 761, 915
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 2 cut(s) 422, 625
Bme18I GGWCC 2 cut(s) 127, 166
BmeT110I CYCGRG 3 cut(s) 560, 869, 1306
BmgBI CACGTC 1 cut(s) 967
BmgT120I GGNCC 2 cut(s) 127, 166
BmiI GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
BmrFI CCNGG 2 cut(s) 422, 625
BmsI GCATC 1 cut(s) 462
BpiI GAAGAC 2 cut(s) 655, 1257
BplI GAGNNNNNCTC 4 cut(s) 8, 40, 554, 586
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 3 cut(s) 1054, 1063, 1271
BpuMI CCSGG 1 cut(s) 625
BsaHI GRCGYC 3 cut(s) 486, 718, 798
BsaJI CCNNGG 3 cut(s) 123, 259, 1323
BsaWI WCCGGW 1 cut(s) 602
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 476, 506, 1065, 1095
Bsc4I CCNNNNNNNGG 7 cut(s) 247, 248, 592, 753, 938, 1057, 1212
Bse118I RCCGGY 1 cut(s) 779
Bse1I ACTGG 1 cut(s) 168
Bse21I CCTNAGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 903
BseAI TCCGGA 1 cut(s) 602
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 3 cut(s) 123, 259, 1323
BseGI GGATG 5 cut(s) 177, 471, 687, 858, 1142
BseLI CCNNNNNNNGG 7 cut(s) 247, 248, 592, 753, 938, 1057, 1212
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 2 cut(s) 516, 585
BseNI ACTGG 1 cut(s) 168
BseRI GAGGAG 2 cut(s) 1060, 1073
BseXI GCAGC 2 cut(s) 207, 407
Bsh1236I CGCG 2 cut(s) 812, 1071
BshFI GGCC 4 cut(s) 521, 686, 977, 1328
BshNI GGYRCC 1 cut(s) 910
BsiHKCI CYCGRG 3 cut(s) 560, 869, 1306
BsiSI CCGG 3 cut(s) 603, 624, 780
BslFI GGGAC 4 cut(s) 454, 497, 955, 1229
BslI CCNNNNNNNGG 7 cut(s) 247, 248, 592, 753, 938, 1057, 1212
BsmAI GTCTC 2 cut(s) 26, 574
BsmBI CGTCTC 1 cut(s) 574
BsmFI GGGAC 4 cut(s) 454, 497, 955, 1229
BsmI GAATGC 2 cut(s) 783, 1324
BsnI GGCC 4 cut(s) 521, 686, 977, 1328
BsoBI CYCGRG 3 cut(s) 560, 869, 1306
Bsp13I TCCGGA 1 cut(s) 602
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 5 cut(s) 627, 891, 928, 1116, 1233
Bsp19I CCATGG 1 cut(s) 123
BspANI GGCC 4 cut(s) 521, 686, 977, 1328
BspCNI CTCAG 2 cut(s) 517, 584
BspEI TCCGGA 1 cut(s) 602
BspFNI CGCG 2 cut(s) 812, 1071
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
BspPI GGATC 4 cut(s) 635, 886, 923, 1241
BspQI GCTCTTC 1 cut(s) 1272
BspT107I GGYRCC 1 cut(s) 910
BsrDI GCAATG 1 cut(s) 903
BsrFI RCCGGY 1 cut(s) 779
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 1 cut(s) 168
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 3 cut(s) 123, 259, 1323
BssMI GATC 5 cut(s) 627, 891, 928, 1116, 1233
BssNI GRCGYC 3 cut(s) 486, 718, 798
BssSI CACGAG 4 cut(s) 531, 726, 847, 1096
BssT1I CCWWGG 2 cut(s) 123, 1323
Bst2BI CACGAG 4 cut(s) 531, 726, 847, 1096
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 1 cut(s) 823
Bst6I CTCTTC 2 cut(s) 247, 1272
BstACI GRCGYC 3 cut(s) 486, 718, 798
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 1168
BstDEI CTNAG 4 cut(s) 226, 357, 525, 571
BstDSI CCRYGG 2 cut(s) 123, 259
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 5 cut(s) 177, 471, 687, 858, 1142
BstFNI CGCG 2 cut(s) 812, 1071
BstKTI GATC 5 cut(s) 630, 894, 931, 1119, 1236
BstMAI GTCTC 2 cut(s) 26, 574
BstMBI GATC 5 cut(s) 627, 891, 928, 1116, 1233
BstMWI GCNNNNNNNGC 3 cut(s) 450, 527, 743
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 712
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 420, 623
BstUI CGCG 2 cut(s) 812, 1071
BstV1I GCAGC 2 cut(s) 207, 407
BstV2I GAAGAC 2 cut(s) 655, 1257
Bsu36I CCTNAGG 1 cut(s) 357
BsuRI GGCC 4 cut(s) 521, 686, 977, 1328
BtgI CCRYGG 2 cut(s) 123, 259
BtgZI GCGATG 1 cut(s) 827
BtrI CACGTC 1 cut(s) 967
BtsCI GGATG 5 cut(s) 177, 471, 687, 858, 1142
BtsIMutI CAGTG 1 cut(s) 819
Cac8I GCNNGC 1 cut(s) 1168
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 2 cut(s) 127, 166
CseI GACGC 8 cut(s) 401, 494, 677, 726, 787, 818, 952, 1133
Csp6I GTAC 4 cut(s) 580, 817, 874, 1000
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
CviQI GTAC 4 cut(s) 580, 817, 874, 1000
DdeI CTNAG 4 cut(s) 226, 357, 525, 571
DpnI GATC 5 cut(s) 629, 893, 930, 1118, 1235
DpnII GATC 5 cut(s) 627, 891, 928, 1116, 1233
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 247, 1272
EarI CTCTTC 2 cut(s) 247, 1272
EciI GGCGGA 2 cut(s) 59, 1286
Eco130I CCWWGG 2 cut(s) 123, 1323
Eco147I AGGCCT 1 cut(s) 1328
Eco47I GGWCC 2 cut(s) 127, 166
Eco81I CCTNAGG 1 cut(s) 357
Eco88I CYCGRG 3 cut(s) 560, 869, 1306
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 2 cut(s) 123, 1323
EcoT22I ATGCAT 1 cut(s) 704
ErhI CCWWGG 2 cut(s) 123, 1323
Esp3I CGTCTC 1 cut(s) 574
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 4 cut(s) 454, 497, 955, 1229
FauI CCCGC 2 cut(s) 241, 1309
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 6 cut(s) 196, 396, 445, 522, 760, 914
FokI GGATG 5 cut(s) 164, 478, 674, 865, 1149
Fsp4HI GCNGC 6 cut(s) 196, 396, 445, 522, 760, 914
FspBI CTAG 2 cut(s) 245, 389
GluI GCNGC 6 cut(s) 196, 396, 445, 522, 760, 914
HaeIII GGCC 4 cut(s) 521, 686, 977, 1328
HapII CCGG 3 cut(s) 603, 624, 780
HgaI GACGC 8 cut(s) 401, 494, 677, 726, 787, 818, 952, 1133
Hin1I GRCGYC 3 cut(s) 486, 718, 798
HinfI GANTC 3 cut(s) 255, 606, 1099
HpaII CCGG 3 cut(s) 603, 624, 780
HphI GGTGA 6 cut(s) 418, 545, 793, 1034, 1075, 1290
Hpy166II GTNNAC 5 cut(s) 259, 819, 838, 876, 991
Hpy188I TCNGA 6 cut(s) 220, 334, 574, 884, 933, 1116
Hpy8I GTNNAC 5 cut(s) 259, 819, 838, 876, 991
Hpy99I CGWCG 6 cut(s) 278, 290, 308, 671, 812, 1127
HpyAV CCTTC 3 cut(s) 284, 439, 757
HpyCH4III ACNGT 1 cut(s) 823
HpyCH4IV ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
HpyCH4V TGCA 6 cut(s) 195, 282, 324, 702, 777, 1015
HpyF10VI GCNNNNNNNGC 3 cut(s) 450, 527, 743
HpyF3I CTNAG 4 cut(s) 226, 357, 525, 571
HpySE526I ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
Hsp92I GRCGYC 3 cut(s) 486, 718, 798
Kpn2I TCCGGA 1 cut(s) 602
Kzo9I GATC 5 cut(s) 627, 891, 928, 1116, 1233
LguI GCTCTTC 1 cut(s) 1272
LmnI GCTCC 5 cut(s) 293, 676, 756, 856, 1047
Lsp1109I GCAGC 2 cut(s) 207, 407
LweI GCATC 1 cut(s) 462
MaeI CTAG 2 cut(s) 245, 389
MaeII ACGT 7 cut(s) 273, 303, 503, 888, 966, 1175, 1209
MaeIII GTNAC 3 cut(s) 424, 799, 1022
MalI GATC 5 cut(s) 629, 893, 930, 1118, 1235
MboI GATC 5 cut(s) 627, 891, 928, 1116, 1233
MboII GAAGA 6 cut(s) 106, 264, 424, 655, 1262, 1289
MluCI AATT 3 cut(s) 1010, 1262, 1336
MlyI GAGTC 2 cut(s) 264, 1108
MmeI TCCRAC 3 cut(s) 77, 243, 1224
Mph1103I ATGCAT 1 cut(s) 704
MroI TCCGGA 1 cut(s) 602
MroXI GAANNNNTTC 1 cut(s) 1281
MseI TTAA 4 cut(s) 960, 981, 1089, 1335
MslI CAYNNNNRTG 5 cut(s) 122, 464, 707, 851, 908
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 3 cut(s) 603, 624, 780
MspR9I CCNGG 2 cut(s) 422, 625
Mva1269I GAATGC 2 cut(s) 783, 1324
MvaI CCWGG 1 cut(s) 422
MvnI CGCG 2 cut(s) 812, 1071
MwoI GCNNNNNNNGC 3 cut(s) 450, 527, 743
NciI CCSGG 1 cut(s) 625
NcoI CCATGG 1 cut(s) 123
NdeI CATATG 1 cut(s) 752
NdeII GATC 5 cut(s) 627, 891, 928, 1116, 1233
NlaIV GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
NmuCI GTSAC 3 cut(s) 424, 799, 1022
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 1 cut(s) 712
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 1 cut(s) 1328
PciI ACATGT 1 cut(s) 708
PciSI GCTCTTC 1 cut(s) 1272
PcsI WCGNNNNNNNCGW 3 cut(s) 282, 724, 1272
PctI GAATGC 2 cut(s) 783, 1324
PdmI GAANNNNTTC 1 cut(s) 1281
PfeI GAWTC 1 cut(s) 606
PkrI GCNGC 6 cut(s) 197, 397, 446, 523, 761, 915
PleI GAGTC 2 cut(s) 263, 1107
PpsI GAGTC 2 cut(s) 263, 1107
PscI ACATGT 1 cut(s) 708
PshBI ATTAAT 1 cut(s) 1335
Psp6I CCWGG 1 cut(s) 420
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 6 cut(s) 265, 295, 758, 858, 912, 1049
PspPI GGNCC 2 cut(s) 127, 166
RsaI GTAC 4 cut(s) 581, 818, 875, 1001
RsaNI GTAC 4 cut(s) 580, 817, 874, 1000
RseI CAYNNNNRTG 5 cut(s) 122, 464, 707, 851, 908
SapI GCTCTTC 1 cut(s) 1272
SaqAI TTAA 4 cut(s) 960, 981, 1089, 1335
SatI GCNGC 6 cut(s) 196, 396, 445, 522, 760, 914
Sau3AI GATC 5 cut(s) 627, 891, 928, 1116, 1233
Sau96I GGNCC 2 cut(s) 127, 166
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 2 cut(s) 264, 1108
ScrFI CCNGG 2 cut(s) 422, 625
SfaNI GCATC 1 cut(s) 462
SinI GGWCC 2 cut(s) 127, 166
SmiMI CAYNNNNRTG 5 cut(s) 122, 464, 707, 851, 908
SmlI CTYRAG 3 cut(s) 1033, 1042, 1286
SmoI CTYRAG 3 cut(s) 1033, 1042, 1286
Sse9I AATT 3 cut(s) 1010, 1262, 1336
SseBI AGGCCT 1 cut(s) 1328
SspMI CTAG 2 cut(s) 245, 389
StuI AGGCCT 1 cut(s) 1328
StyD4I CCNGG 2 cut(s) 420, 623
StyI CCWWGG 2 cut(s) 123, 1323
TaaI ACNGT 1 cut(s) 823
TaiI ACGT 7 cut(s) 276, 306, 506, 891, 969, 1178, 1212
TaqI TCGA 8 cut(s) 93, 276, 306, 342, 384, 666, 1266, 1331
TaqII GACCGA 1 cut(s) 332
TasI AATT 3 cut(s) 1010, 1262, 1336
TatI WGTACW 4 cut(s) 579, 816, 873, 999
TauI GCSGC 4 cut(s) 447, 524, 762, 916
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 4 cut(s) 960, 981, 1089, 1335
Tru9I TTAA 4 cut(s) 960, 981, 1089, 1335
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 3 cut(s) 424, 799, 1022
TseI GCWGC 2 cut(s) 195, 395
Tsp45I GTSAC 3 cut(s) 424, 799, 1022
TspDTI ATGAA 4 cut(s) 17, 106, 739, 938
TspGWI ACGGA 3 cut(s) 276, 1041, 1233
TspRI CASTG 1 cut(s) 826
VpaK11BI GGWCC 2 cut(s) 127, 166
VspI ATTAAT 1 cut(s) 1335
XapI RAATTY 1 cut(s) 1262
XceI RCATGY 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 990
XmnI GAANNNNTTC 1 cut(s) 1281
XspI CTAG 2 cut(s) 245, 389
ZrmI AGTACT 1 cut(s) 581
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.