Rw4G026860

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000852
Physical Location & Seq
Reverse (-)
53132487 .. 53133823
1337 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026860.1

Sequence Viewer

Length: 1296 bp
ATGAAGGTTGAGGTTGAGGTTGTCACCAGAGATCTTATCAGACCATCTTCTCCAACCCCTGACCACCTCCACAGATATCAACTCTCCTTTCTTGACCAGATATCTCCCCCAGTCTTTAGTCCTTTGGTTCTATTTTATTCATCATCCGAGGCTGATACAAATTTTGACATTGCTGATATATCGAATCGGCTAAAGAAATCCCTCTCCGATGTCTTGGTCCAATTCTACCCACTTGCTGGACGAGCAAATGACAACATGTTCATAGACTGCAACGACAAGGGCATACCCTTCTTGGAAACTCGAGTTAACTGCCAACTATCTGATGTTATCCAAAACCCAGTTCCCAGTGAACTCAACAAGTTGGTTCCATTTCAACTAGACGAAGTTGCTGATCTAGCCCTTGGTGTCCAACTAAATGTCTTTGACTGTGGTGGAGTTGCAATTGGTGCTTGCATTTCTCACAAGATTGCAGATGCTTTATCGTTTTTCATGTTCATCAAAACTTGGGCAGCCACTGCTAGAAAAGATCATTATCATCAGACAGATGATCATGCAGTTATGTCTCCACAGTTTGTATCAGCCACACTGTTCCCACCAAGGAACATATCCGGGTTTAATCCACGTGTTGGGATCGAAAAAGAGAATATCGTAACAAAGAGGTTTGTGTTTGCTGCTTCCAAGATAGATTCTCTTAAATCAAAATATGGTTTACCAAAGACCGCCATCTCGTGTGGAGGCTCTATCGCTTTCATATGGAGTCGCTATGTGGCTGCAACCGAAGTTGGGTCTGAAGCACCTGAGAGGGTCTATGCCATTGTTCATGCTGTGAACCTCAGGACAAGGTTTGATCCACCACTGTCAGAATGTTCTTTCGGGAATCTCTACCGAATTGCAGTTACGGTTCCACGTCTGGATACAGGGGAGGAGTGCAATGGTCTTGTGAGGCAGGTCAGAGAGCAAGTGAGTCATATTGACAAGGATTATGTGAAGAAATTGCAGGAGGGCAGTGAACACTTGAGTTTCATCAAACAAAGCTCTGATAATTTCTACAAGGGAGAGATGGTTACACTGAGCTTCACAAGTCTGTGCAGATTTCCTCTGTATGAAGCTGATTTTGGATGGGGAAAGCCTACTTGGGTCGGCTCTCCTGCCCTGACTTTCAATAATTTGGTTGTTTTTATAGACACTAAATCTGGGCGTGGAATAGAAGCATACATTAACATGAAGGAAGGAGATTTGGCTAAAATTGAATCTGACAAAGAGTTCATTTCATTTGTTTCAAGTGAGTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

431

Amino Acids

48.1

Weight (kDa)

5.79

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 422 5.3e-73 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 937
AccB7I CCANNNNNTGG 2 cut(s) 236, 626
AciI CCGC 1 cut(s) 720
AclWI GGATC 2 cut(s) 638, 842
AcsI RAATTY 1 cut(s) 160
AcuI CTGAAG 1 cut(s) 810
AcvI CACGTG 1 cut(s) 623
AfiI CCNNNNNNNGG 3 cut(s) 236, 626, 783
AflIII ACRYGT 2 cut(s) 255, 622
AgsI TTSAA 4 cut(s) 374, 1162, 1250, 1281
AjiI CACGTC 1 cut(s) 908
AjuI GAANNNNNNNTTGG 6 cut(s) 324, 356, 1098, 1117, 1130, 1149
AluBI AGCT 3 cut(s) 1035, 1074, 1109
AluI AGCT 3 cut(s) 1035, 1074, 1109
Alw26I GTCTC 1 cut(s) 567
AlwI GGATC 2 cut(s) 638, 842
AlwNI CAGNNNCTG 1 cut(s) 515
Ama87I CYCGRG 1 cut(s) 300
ApeKI GCWGC 3 cut(s) 509, 671, 770
ApoI RAATTY 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 217
AsuC2I CCSGG 1 cut(s) 610
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 1 cut(s) 217
AxyI CCTNAGG 1 cut(s) 833
BauI CACGAG 1 cut(s) 727
BbrPI CACGTG 1 cut(s) 623
BbvI GCAGC 3 cut(s) 521, 658, 757
BccI CCATC 4 cut(s) 52, 731, 1054, 1113
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BciVI GTATCC 1 cut(s) 907
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 1 cut(s) 610
BcoDI GTCTC 1 cut(s) 567
BfaI CTAG 3 cut(s) 377, 395, 519
BfuAI ACCTGC 1 cut(s) 937
BfuI GTATCC 1 cut(s) 907
BglII AGATCT 1 cut(s) 31
BisI GCNGC 3 cut(s) 510, 672, 771
BlsI GCNGC 3 cut(s) 511, 673, 772
Bme1390I CCNGG 1 cut(s) 610
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 300
BmgBI CACGTC 1 cut(s) 908
BmgT120I GGNCC 1 cut(s) 217
BmiI GGNNCC 2 cut(s) 366, 903
BmrFI CCNGG 1 cut(s) 610
BmrI ACTGGG 3 cut(s) 104, 332, 339
BmsI GCATC 1 cut(s) 463
BmuI ACTGGG 3 cut(s) 104, 332, 339
BpuEI CTTGAG 1 cut(s) 1036
BpuMI CCSGG 1 cut(s) 610
BsaAI YACGTR 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 531
BsaJI CCNNGG 3 cut(s) 147, 400, 596
BsaXI ACNNNNNCTCC 2 cut(s) 1047, 1077
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 626, 783
Bse1I ACTGG 3 cut(s) 110, 338, 345
Bse21I CCTNAGG 1 cut(s) 833
Bse3DI GCAATG 2 cut(s) 168, 937
Bse8I GATNNNNATC 1 cut(s) 531
BseDI CCNNGG 3 cut(s) 147, 400, 596
BseGI GGATG 2 cut(s) 143, 1124
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 3 cut(s) 236, 626, 783
BseMI GCAATG 2 cut(s) 168, 937
BseMII CTCAG 3 cut(s) 789, 847, 1061
BseNI ACTGG 3 cut(s) 110, 338, 345
BseRI GAGGAG 1 cut(s) 938
BseXI GCAGC 3 cut(s) 521, 658, 757
BsgI GTGCAG 1 cut(s) 1108
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 1 cut(s) 609
BslI CCNNNNNNNGG 3 cut(s) 236, 626, 783
BsmAI GTCTC 1 cut(s) 567
BsoBI CYCGRG 1 cut(s) 300
Bsp143I GATC 6 cut(s) 31, 391, 526, 547, 630, 847
BspACI CCGC 1 cut(s) 720
BspCNI CTCAG 3 cut(s) 790, 846, 1062
BspLI GGNNCC 2 cut(s) 366, 903
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 2 cut(s) 638, 842
BsrDI GCAATG 2 cut(s) 168, 937
BsrI ACTGG 3 cut(s) 110, 338, 345
BssECI CCNNGG 3 cut(s) 147, 400, 596
BssMI GATC 6 cut(s) 31, 391, 526, 547, 630, 847
BssSI CACGAG 1 cut(s) 727
BssT1I CCWWGG 2 cut(s) 400, 596
Bst2BI CACGAG 1 cut(s) 727
Bst4CI ACNGT 5 cut(s) 428, 570, 588, 858, 901
BstAPI GCANNNNNTGC 2 cut(s) 446, 515
BstBAI YACGTR 1 cut(s) 623
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 3 cut(s) 798, 833, 1070
BstF5I GGATG 2 cut(s) 143, 1124
BstKTI GATC 6 cut(s) 34, 394, 529, 550, 633, 850
BstMAI GTCTC 1 cut(s) 567
BstMBI GATC 6 cut(s) 31, 391, 526, 547, 630, 847
BstMWI GCNNNNNNNGC 4 cut(s) 242, 395, 446, 515
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 608
BstV1I GCAGC 3 cut(s) 521, 658, 757
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
Bsu36I CCTNAGG 1 cut(s) 833
BsuI GTATCC 1 cut(s) 907
BtrI CACGTC 1 cut(s) 908
BtsCI GGATG 2 cut(s) 143, 1124
BtsI GCAGTG 2 cut(s) 513, 1012
BtsIMutI CAGTG 6 cut(s) 352, 513, 584, 854, 1012, 1067
BveI ACCTGC 1 cut(s) 937
Cac8I GCNNGC 1 cut(s) 451
CaiI CAGNNNCTG 1 cut(s) 515
Cfr13I GGNCC 1 cut(s) 217
CviAII CATG 5 cut(s) 256, 490, 551, 821, 1222
DdeI CTNAG 3 cut(s) 798, 833, 1070
DpnI GATC 6 cut(s) 33, 393, 528, 549, 632, 849
DpnII GATC 6 cut(s) 31, 391, 526, 547, 630, 847
Eco130I CCWWGG 2 cut(s) 400, 596
Eco32I GATATC 2 cut(s) 77, 102
Eco47I GGWCC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 810
Eco72I CACGTG 1 cut(s) 623
Eco81I CCTNAGG 1 cut(s) 833
Eco88I CYCGRG 1 cut(s) 300
EcoRV GATATC 2 cut(s) 77, 102
EcoT14I CCWWGG 2 cut(s) 400, 596
ErhI CCWWGG 2 cut(s) 400, 596
FaeI CATG 5 cut(s) 259, 493, 554, 824, 1225
FatI CATG 5 cut(s) 255, 489, 550, 820, 1221
FauNDI CATATG 1 cut(s) 752
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 3 cut(s) 510, 672, 771
FokI GGATG 2 cut(s) 130, 1131
Fsp4HI GCNGC 3 cut(s) 510, 672, 771
FspBI CTAG 3 cut(s) 377, 395, 519
GluI GCNGC 3 cut(s) 510, 672, 771
HapII CCGG 1 cut(s) 609
Hin1II CATG 5 cut(s) 259, 493, 554, 824, 1225
HincII GTYRAC 1 cut(s) 307
HindII GTYRAC 1 cut(s) 307
HinfI GANTC 7 cut(s) 184, 686, 757, 877, 964, 1250, 1286
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 1 cut(s) 609
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 5 cut(s) 307, 350, 710, 829, 1010
Hpy188III TCNNGA 4 cut(s) 92, 835, 874, 911
Hpy8I GTNNAC 5 cut(s) 307, 350, 710, 829, 1010
HpyAV CCTTC 3 cut(s) 298, 1219, 1223
HpyCH4III ACNGT 5 cut(s) 428, 570, 588, 858, 901
HpyCH4IV ACGT 2 cut(s) 622, 907
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 395, 446, 515
HpyF3I CTNAG 3 cut(s) 798, 833, 1070
HpySE526I ACGT 2 cut(s) 622, 907
Hsp92II CATG 5 cut(s) 259, 493, 554, 824, 1225
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 6 cut(s) 31, 391, 526, 547, 630, 847
Lsp1109I GCAGC 3 cut(s) 521, 658, 757
LweI GCATC 1 cut(s) 463
MaeI CTAG 3 cut(s) 377, 395, 519
MaeII ACGT 2 cut(s) 622, 907
MaeIII GTNAC 4 cut(s) 22, 649, 895, 1063
MalI GATC 6 cut(s) 33, 393, 528, 549, 632, 849
MboI GATC 6 cut(s) 31, 391, 526, 547, 630, 847
MboII GAAGA 2 cut(s) 39, 1000
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 31
MluCI AATT 8 cut(s) 160, 221, 441, 888, 992, 1042, 1165, 1245
MlyI GAGTC 3 cut(s) 766, 973, 1295
MmeI TCCRAC 2 cut(s) 77, 433
MseI TTAA 4 cut(s) 306, 615, 693, 1218
MslI CAYNNNNRTG 1 cut(s) 1220
MspI CCGG 1 cut(s) 609
MspR9I CCNGG 1 cut(s) 610
MunI CAATTG 1 cut(s) 441
MwoI GCNNNNNNNGC 4 cut(s) 242, 395, 446, 515
NciI CCSGG 1 cut(s) 610
NdeI CATATG 1 cut(s) 752
NdeII GATC 6 cut(s) 31, 391, 526, 547, 630, 847
NlaIII CATG 5 cut(s) 259, 493, 554, 824, 1225
NlaIV GGNNCC 2 cut(s) 366, 903
NmuCI GTSAC 1 cut(s) 22
NspI RCATGY 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 300
PciI ACATGT 1 cut(s) 255
PfeI GAWTC 4 cut(s) 184, 686, 877, 1250
PflMI CCANNNNNTGG 2 cut(s) 236, 626
PkrI GCNGC 3 cut(s) 511, 673, 772
PleI GAGTC 3 cut(s) 765, 972, 1294
PmaCI CACGTG 1 cut(s) 623
PmlI CACGTG 1 cut(s) 623
PpsI GAGTC 3 cut(s) 765, 972, 1294
Ppu21I YACGTR 1 cut(s) 623
PscI ACATGT 1 cut(s) 255
PspCI CACGTG 1 cut(s) 623
PspN4I GGNNCC 2 cut(s) 366, 903
PspPI GGNCC 1 cut(s) 217
PspXI VCTCGAGB 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 1220
SaqAI TTAA 4 cut(s) 306, 615, 693, 1218
SatI GCNGC 3 cut(s) 510, 672, 771
Sau3AI GATC 6 cut(s) 31, 391, 526, 547, 630, 847
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 3 cut(s) 766, 973, 1295
ScrFI CCNGG 1 cut(s) 610
SfaNI GCATC 1 cut(s) 463
Sfr274I CTCGAG 1 cut(s) 300
SinI GGWCC 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 1220
SmlI CTYRAG 2 cut(s) 300, 1015
SmoI CTYRAG 2 cut(s) 300, 1015
Sse9I AATT 8 cut(s) 160, 221, 441, 888, 992, 1042, 1165, 1245
SsiI CCGC 1 cut(s) 720
SspMI CTAG 3 cut(s) 377, 395, 519
StyD4I CCNGG 1 cut(s) 608
StyI CCWWGG 2 cut(s) 400, 596
TaaI ACNGT 5 cut(s) 428, 570, 588, 858, 901
TaiI ACGT 2 cut(s) 625, 910
TaqI TCGA 3 cut(s) 182, 301, 633
TasI AATT 8 cut(s) 160, 221, 441, 888, 992, 1042, 1165, 1245
TfiI GAWTC 4 cut(s) 184, 686, 877, 1250
Tru1I TTAA 4 cut(s) 306, 615, 693, 1218
Tru9I TTAA 4 cut(s) 306, 615, 693, 1218
TscAI CASTG 6 cut(s) 352, 520, 591, 861, 1012, 1074
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 3 cut(s) 509, 671, 770
Tsp45I GTSAC 1 cut(s) 22
TspRI CASTG 6 cut(s) 352, 520, 591, 861, 1012, 1074
Van91I CCANNNNNTGG 2 cut(s) 236, 626
VpaK11BI GGWCC 1 cut(s) 217
XapI RAATTY 1 cut(s) 160
XceI RCATGY 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 300
XspI CTAG 3 cut(s) 377, 395, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.