Prupe.1G236500_v2.0.a1

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000852
Physical Location & Seq
Reverse (-)
25058367 .. 25059677
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G236500.1

Sequence Viewer

Length: 1311 bp
ATGAAGGTTGAAGTTGAAGTAATCTCCAAAGAGATTATCAAACCATCTTCACCAACTCCTGATCACCTTCGCACATACCACCTCTCCTTTCTTGACCAGATATCTCCCCCGGTCCATAATCCTTTGGTCATATTCTATTCCTCAGAGCCCGATACAAAATTCAATATTGTTGACATATCCAATCAGCTCAAGAAATCCCTCTCCGATGTCTTAACTCTATTCTACCCACTAGCCGGACGAGCCCATGGCAACTTGTTCATTGATTGCAATGATGAGGGCATACCATACTTGGAAACTCGAGTTAATTGTCAACTCTCTCACGTCATCCAAAACCCAATTCCAAGTGAACTCAACAAATTCATTCCATTTCAAGTACATGAAGTTGCTGATCTTTCCCTTGGTGTCCAACTCAACATCTTTGACTGTGGTGGCATAGCTCTTGGTGCTTGCGTTTCCCACAAGATTGCAGATGCTTTGTCACTTTTCATGTTCATCAAAAGTTGGGCAGCCATTTCCAGACAAGAAGATGAACATATAGTGTGCCCCCAATTTGTATCAGCCACACTCTTCCCACCAAGAAACATATCCGGGTTTAATCCAAGGACTGGTATCACAAAGCAGAATGTTGTAACAAAGAGATTTATCTTTGATGCTTCAAAGATAGAGGCTCTTAAAGCAAAATATAGTATTGATAATTACAGCCAAAGCTTAGAAAGCTACCAAAAACCCCCTTCTCGTGTGGAAGCTTTGTCAGCCTTCATATGGAGCCGCGTTGTGGCAAGCACTCAAGTGAAGTCAGAACCGGCACCCGAGAGGCTGTATGCCATTGTTCATGCAGTGAACCTACGTACAAGAATTGACCCTCCTCTGCCTGAACACTCATTTGGAAATCTCTACAGAATTGCAATGACAATCCCATCGCTGCAAACAGGGGAGGAGTGCTATGGCCTCGTGAGGCAGGTGAGGGACCAAGTGAGACAAATCGACAAACACTACGTCAGCAAACTGCAGCAGGGCAGTGAGCACTTGGATTTCATCAAACAGAGCTCTGAGAACTGCTTCAAGGGAGAGATGGTGACGCTGAGCTTCACTAGTCTGTGTAGATTTCCTCTGTACGAAGCTGATTTTGGGTGGGGAAAACCCACTTGGGTTGGCTCACCTCCCCTGACTTTCAATAACTTGGTTGTTTTCATGGACGCCAAGTCTGGTGGTGGAATAGAAGCATATATTAACATGAAGGAAGAGGACTTGGCTAAACTTGAAGGTGACAATGAGTTCCTTGCATGTGTTTCTCCACATGGGTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

437

Amino Acids

48.94

Weight (kDa)

6.13

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 949
Acc36I ACCTGC 1 cut(s) 949
AccB1I GGYRCC 1 cut(s) 805
AccB7I CCANNNNNTGG 1 cut(s) 605
AccII CGCG 1 cut(s) 771
AciI CCGC 1 cut(s) 769
AcsI RAATTY 2 cut(s) 158, 356
AcyI GRCGYC 1 cut(s) 1197
AfaI GTAC 3 cut(s) 375, 850, 1115
AfiI CCNNNNNNNGG 4 cut(s) 233, 605, 762, 775
AgsI TTSAA 9 cut(s) 11, 17, 163, 371, 657, 1063, 1174, 1262, 1306
AhdI GACNNNNNGTC 1 cut(s) 1201
AhlI ACTAGT 1 cut(s) 1091
AjiI CACGTC 1 cut(s) 322
AjuI GAANNNNNNNTTGG 8 cut(s) 321, 353, 867, 899, 1110, 1129, 1142, 1161
AleI CACNNNNGTG 1 cut(s) 788
AluBI AGCT 8 cut(s) 187, 437, 708, 717, 746, 1047, 1086, 1121
AluI AGCT 8 cut(s) 187, 437, 708, 717, 746, 1047, 1086, 1121
Alw21I GWGCWC 2 cut(s) 1026, 1049
Alw26I GTCTC 1 cut(s) 970
Ama87I CYCGRG 2 cut(s) 297, 809
AoxI GGCC 1 cut(s) 946
ApeKI GCWGC 3 cut(s) 506, 922, 1009
ApoI RAATTY 2 cut(s) 158, 356
ArsI GACNNNNNNTTYG 2 cut(s) 981, 1013
Asp700I GAANNNNTTC 1 cut(s) 1058
AspS9I GGNCC 2 cut(s) 112, 967
AsuC2I CCSGG 2 cut(s) 110, 589
AsuHPI GGTGA 6 cut(s) 42, 56, 973, 1087, 1149, 1277
AvaI CYCGRG 2 cut(s) 297, 809
AvaII GGWCC 2 cut(s) 112, 967
BaeGI GKGCMC 1 cut(s) 545
BanI GGYRCC 1 cut(s) 805
BanII GRGCYC 3 cut(s) 150, 244, 1049
BauI CACGAG 2 cut(s) 735, 950
Bbv12I GWGCWC 2 cut(s) 1026, 1049
BbvI GCAGC 3 cut(s) 518, 909, 1021
BccI CCATC 3 cut(s) 52, 925, 1066
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 2 cut(s) 110, 589
BcoDI GTCTC 1 cut(s) 970
BcuI ACTAGT 1 cut(s) 1091
BfaI CTAG 2 cut(s) 230, 1092
BfmI CTRYAG 2 cut(s) 895, 1007
BfuAI ACCTGC 1 cut(s) 949
BisI GCNGC 4 cut(s) 507, 769, 923, 1010
BlpI GCTNAGC 1 cut(s) 1082
BlsI GCNGC 4 cut(s) 508, 770, 924, 1011
Bme1390I CCNGG 2 cut(s) 110, 589
Bme18I GGWCC 2 cut(s) 112, 967
BmeRI GACNNNNNGTC 1 cut(s) 1201
BmeT110I CYCGRG 2 cut(s) 297, 809
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 2 cut(s) 112, 967
BmiI GGNNCC 3 cut(s) 767, 807, 968
BmrFI CCNGG 2 cut(s) 110, 589
BmsI GCATC 2 cut(s) 460, 640
Bpu1102I GCTNAGC 1 cut(s) 1082
BpuEI CTTGAG 2 cut(s) 173, 771
BpuMI CCSGG 2 cut(s) 110, 589
BsaAI YACGTR 1 cut(s) 848
BsaHI GRCGYC 1 cut(s) 1197
BsaJI CCNNGG 4 cut(s) 108, 244, 397, 599
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1059, 1089
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 605, 762, 775
Bse118I RCCGGY 1 cut(s) 802
Bse1I ACTGG 1 cut(s) 610
Bse3DI GCAATG 2 cut(s) 274, 912
BseDI CCNNGG 4 cut(s) 108, 244, 397, 599
BseGI GGATG 1 cut(s) 324
BseLI CCNNNNNNNGG 4 cut(s) 233, 605, 762, 775
BseMI GCAATG 2 cut(s) 274, 912
BseMII CTCAG 3 cut(s) 156, 1041, 1073
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 2 cut(s) 855, 950
BseSI GKGCMC 1 cut(s) 545
BseXI GCAGC 3 cut(s) 518, 909, 1021
Bsh1236I CGCG 1 cut(s) 771
BshFI GGCC 1 cut(s) 948
BshNI GGYRCC 1 cut(s) 805
BsiHKAI GWGCWC 2 cut(s) 1026, 1049
BsiHKCI CYCGRG 2 cut(s) 297, 809
BsiSI CCGG 4 cut(s) 110, 234, 588, 803
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 4 cut(s) 233, 605, 762, 775
BsmAI GTCTC 1 cut(s) 970
BsmFI GGGAC 1 cut(s) 980
BsnI GGCC 1 cut(s) 948
BsoBI CYCGRG 2 cut(s) 297, 809
Bsp1286I GDGCHC 5 cut(s) 150, 244, 545, 1026, 1049
Bsp143I GATC 2 cut(s) 61, 388
Bsp1720I GCTNAGC 1 cut(s) 1082
Bsp19I CCATGG 1 cut(s) 244
BspACI CCGC 1 cut(s) 769
BspANI GGCC 1 cut(s) 948
BspCNI CTCAG 3 cut(s) 155, 1042, 1074
BspFNI CGCG 1 cut(s) 771
BspLI GGNNCC 3 cut(s) 767, 807, 968
BspMAI CTGCAG 1 cut(s) 1011
BspMI ACCTGC 1 cut(s) 949
BspT107I GGYRCC 1 cut(s) 805
BsrDI GCAATG 2 cut(s) 274, 912
BsrFI RCCGGY 1 cut(s) 802
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 4 cut(s) 108, 244, 397, 599
BssMI GATC 2 cut(s) 61, 388
BssNI GRCGYC 1 cut(s) 1197
BssSI CACGAG 2 cut(s) 735, 950
BssT1I CCWWGG 3 cut(s) 244, 397, 599
Bst2BI CACGAG 2 cut(s) 735, 950
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 2 cut(s) 572, 1236
BstACI GRCGYC 1 cut(s) 1197
BstBAI YACGTR 1 cut(s) 848
BstC8I GCNNGC 2 cut(s) 448, 781
BstDEI CTNAG 4 cut(s) 142, 709, 1050, 1082
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 771
BstKTI GATC 2 cut(s) 64, 391
BstMAI GTCTC 1 cut(s) 970
BstMBI GATC 2 cut(s) 61, 388
BstMWI GCNNNNNNNGC 5 cut(s) 239, 443, 674, 714, 752
BstNSI RCATGY 1 cut(s) 1287
BstSCI CCNGG 2 cut(s) 108, 587
BstSFI CTRYAG 2 cut(s) 895, 1007
BstSLI GKGCMC 1 cut(s) 545
BstSNI TACGTA 1 cut(s) 848
BstUI CGCG 1 cut(s) 771
BstV1I GCAGC 3 cut(s) 518, 909, 1021
BsuRI GGCC 1 cut(s) 948
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 903
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 1 cut(s) 324
BtsI GCAGTG 2 cut(s) 843, 1024
BtsIMutI CAGTG 2 cut(s) 843, 1024
BveI ACCTGC 1 cut(s) 949
Cac8I GCNNGC 2 cut(s) 448, 781
Cfr10I RCCGGY 1 cut(s) 802
Cfr13I GGNCC 2 cut(s) 112, 967
CseI GACGC 2 cut(s) 1087, 1205
Csp6I GTAC 3 cut(s) 374, 849, 1114
CspCI CAANNNNNGTGG 4 cut(s) 1132, 1167, 1189, 1224
CviAII CATG 8 cut(s) 245, 377, 487, 833, 1192, 1234, 1284, 1298
CviQI GTAC 3 cut(s) 374, 849, 1114
DdeI CTNAG 4 cut(s) 142, 709, 1050, 1082
DpnI GATC 2 cut(s) 63, 390
DpnII GATC 2 cut(s) 61, 388
DriI GACNNNNNGTC 1 cut(s) 1201
Eam1104I CTCTTC 2 cut(s) 572, 1236
Eam1105I GACNNNNNGTC 1 cut(s) 1201
EarI CTCTTC 2 cut(s) 572, 1236
Ecl136II GAGCTC 1 cut(s) 1047
Eco105I TACGTA 1 cut(s) 848
Eco130I CCWWGG 3 cut(s) 244, 397, 599
Eco24I GRGCYC 3 cut(s) 150, 244, 1049
Eco32I GATATC 1 cut(s) 102
Eco47I GGWCC 2 cut(s) 112, 967
Eco53kI GAGCTC 1 cut(s) 1047
Eco88I CYCGRG 2 cut(s) 297, 809
EcoICRI GAGCTC 1 cut(s) 1047
EcoRV GATATC 1 cut(s) 102
EcoT14I CCWWGG 3 cut(s) 244, 397, 599
EcoT38I GRGCYC 3 cut(s) 150, 244, 1049
ErhI CCWWGG 3 cut(s) 244, 397, 599
FaeI CATG 8 cut(s) 248, 380, 490, 836, 1195, 1237, 1287, 1301
FaqI GGGAC 1 cut(s) 980
FatI CATG 8 cut(s) 244, 376, 486, 832, 1191, 1233, 1283, 1297
FauNDI CATATG 1 cut(s) 761
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 4 cut(s) 507, 769, 923, 1010
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 3 cut(s) 150, 244, 1049
Fsp4HI GCNGC 4 cut(s) 507, 769, 923, 1010
FspBI CTAG 2 cut(s) 230, 1092
GluI GCNGC 4 cut(s) 507, 769, 923, 1010
HaeIII GGCC 1 cut(s) 948
HapII CCGG 4 cut(s) 110, 234, 588, 803
HgaI GACGC 2 cut(s) 1087, 1205
Hin1I GRCGYC 1 cut(s) 1197
Hin1II CATG 8 cut(s) 248, 380, 490, 836, 1195, 1237, 1287, 1301
HincII GTYRAC 2 cut(s) 172, 311
HindII GTYRAC 2 cut(s) 172, 311
HindIII AAGCTT 2 cut(s) 706, 744
HpaII CCGG 4 cut(s) 110, 234, 588, 803
HphI GGTGA 6 cut(s) 42, 56, 973, 1087, 1149, 1277
Hpy166II GTNNAC 4 cut(s) 172, 311, 347, 841
Hpy188I TCNGA 4 cut(s) 145, 205, 799, 1051
Hpy188III TCNNGA 5 cut(s) 59, 92, 190, 516, 952
Hpy8I GTNNAC 4 cut(s) 172, 311, 347, 841
HpyAV CCTTC 5 cut(s) 77, 741, 766, 1231, 1256
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 3 cut(s) 321, 847, 996
HpyCH4V TGCA 7 cut(s) 267, 467, 836, 905, 925, 1009, 1283
HpyF10VI GCNNNNNNNGC 5 cut(s) 239, 443, 674, 714, 752
HpyF3I CTNAG 4 cut(s) 142, 709, 1050, 1082
HpySE526I ACGT 3 cut(s) 321, 847, 996
Hsp92I GRCGYC 1 cut(s) 1197
Hsp92II CATG 8 cut(s) 248, 380, 490, 836, 1195, 1237, 1287, 1301
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 2 cut(s) 61, 388
LmnI GCTCC 1 cut(s) 765
Lsp1109I GCAGC 3 cut(s) 518, 909, 1021
LweI GCATC 2 cut(s) 460, 640
MaeI CTAG 2 cut(s) 230, 1092
MaeII ACGT 3 cut(s) 321, 847, 996
MaeIII GTNAC 4 cut(s) 477, 628, 1075, 1265
MalI GATC 2 cut(s) 63, 390
MboI GATC 2 cut(s) 61, 388
MboII GAAGA 4 cut(s) 39, 536, 559, 1253
MhlI GDGCHC 5 cut(s) 150, 244, 545, 1026, 1049
MluCI AATT 8 cut(s) 158, 304, 336, 356, 548, 694, 855, 900
MmeI TCCRAC 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 1058
MseI TTAA 5 cut(s) 212, 303, 594, 672, 1230
MslI CAYNNNNRTG 1 cut(s) 788
MspI CCGG 4 cut(s) 110, 234, 588, 803
MspR9I CCNGG 2 cut(s) 110, 589
MvnI CGCG 1 cut(s) 771
MwoI GCNNNNNNNGC 5 cut(s) 239, 443, 674, 714, 752
NciI CCSGG 2 cut(s) 110, 589
NcoI CCATGG 1 cut(s) 244
NdeI CATATG 1 cut(s) 761
NdeII GATC 2 cut(s) 61, 388
NlaIII CATG 8 cut(s) 248, 380, 490, 836, 1195, 1237, 1287, 1301
NlaIV GGNNCC 3 cut(s) 767, 807, 968
NmuCI GTSAC 3 cut(s) 477, 1075, 1265
NspI RCATGY 1 cut(s) 1287
OliI CACNNNNGTG 1 cut(s) 788
PaeR7I CTCGAG 1 cut(s) 297
PaqCI CACCTGC 1 cut(s) 949
PdmI GAANNNNTTC 1 cut(s) 1058
PflMI CCANNNNNTGG 1 cut(s) 605
PkrI GCNGC 4 cut(s) 508, 770, 924, 1011
Ppu21I YACGTR 1 cut(s) 848
Psp124BI GAGCTC 1 cut(s) 1049
PspN4I GGNNCC 3 cut(s) 767, 807, 968
PspPI GGNCC 2 cut(s) 112, 967
PspXI VCTCGAGB 1 cut(s) 297
PstI CTGCAG 1 cut(s) 1011
RsaI GTAC 3 cut(s) 375, 850, 1115
RsaNI GTAC 3 cut(s) 374, 849, 1114
RseI CAYNNNNRTG 1 cut(s) 788
SacI GAGCTC 1 cut(s) 1049
SaqAI TTAA 5 cut(s) 212, 303, 594, 672, 1230
SatI GCNGC 4 cut(s) 507, 769, 923, 1010
Sau3AI GATC 2 cut(s) 61, 388
Sau96I GGNCC 2 cut(s) 112, 967
ScrFI CCNGG 2 cut(s) 110, 589
SduI GDGCHC 5 cut(s) 150, 244, 545, 1026, 1049
SfaNI GCATC 2 cut(s) 460, 640
SfcI CTRYAG 2 cut(s) 895, 1007
Sfr274I CTCGAG 1 cut(s) 297
SinI GGWCC 2 cut(s) 112, 967
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 3 cut(s) 188, 297, 786
SmoI CTYRAG 3 cut(s) 188, 297, 786
SnaBI TACGTA 1 cut(s) 848
SpeI ACTAGT 1 cut(s) 1091
Sse9I AATT 8 cut(s) 158, 304, 336, 356, 548, 694, 855, 900
SsiI CCGC 1 cut(s) 769
SspI AATATT 1 cut(s) 166
SspMI CTAG 2 cut(s) 230, 1092
SstI GAGCTC 1 cut(s) 1049
StyD4I CCNGG 2 cut(s) 108, 587
StyI CCWWGG 3 cut(s) 244, 397, 599
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 3 cut(s) 324, 850, 999
TaqI TCGA 2 cut(s) 298, 984
TasI AATT 8 cut(s) 158, 304, 336, 356, 548, 694, 855, 900
TatI WGTACW 1 cut(s) 373
TauI GCSGC 1 cut(s) 771
Tru1I TTAA 5 cut(s) 212, 303, 594, 672, 1230
Tru9I TTAA 5 cut(s) 212, 303, 594, 672, 1230
TscAI CASTG 2 cut(s) 843, 1024
TseFI GTSAC 3 cut(s) 477, 1075, 1265
TseI GCWGC 3 cut(s) 506, 922, 1009
Tsp45I GTSAC 3 cut(s) 477, 1075, 1265
TspRI CASTG 2 cut(s) 843, 1024
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 2 cut(s) 112, 967
XapI RAATTY 2 cut(s) 158, 356
XceI RCATGY 1 cut(s) 1287
XhoI CTCGAG 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 1058
XspI CTAG 2 cut(s) 230, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.