Rroxscaffold_4G00317340

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000852
Physical Location & Seq
Reverse (-)
42570917 .. 42572293
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00317340.1

Sequence Viewer

Length: 1323 bp
ATGAAGTTTCAAGTGGAGGTTCTCTCAACTGAGACAGTCAAACCCTCATCTCCAACCCCTGACCACCTCCGCCACCACAAGCTCTCCTACCTCGACCAAATCCAGCCCCCAATCTTCATGCCCATGGTCCTCTTCTACCCGAAAAAACCCGACCACAACGACCTGGACCTCATTTCCATCCATGAACAGCGTTGCAGCAAAATCAAGAAGGCATTGTCCGACATCTTAGTCAAGTTCTACCCACTAGCCGGAAGAGTCCACAGAACCCAATATGTCGATTGCAACGACGAAGGAGCGTACTACGTCGAGGCCAAGACCGACTGCAAAATCTCTGACATCATCGACAACCCAAACTCTAAGGACTTCAACAAGTTCCTTCCTTTCGAGCTAGACGCAGCTCATGAGCTTCCTGTGTGCTTGCAGGTCACCTTCTTCGCCTGCGGCGGCATGAGCATCGCCATGGGGATATCCCACAAGGTCGGGGACGCCCTCTCCTACGTCACGTTTCTCAATGGCCTGGCCGCTGAGGCTCGTGGGAAGGGCAACGATATTCACCCTCCCGAGTTCGTCTCAGACAAGTACTTCCCCCAAAAAGACTTGTCCGGATTCTTCCAACACCGCACCGGGATCATAAAGCAAAACATCTCAACAAAACGCTTCGTCTTCGACGCTCCAATGGTTCAGGCCATCCGAGCCAAATGCGAGGACACGTCCAGACGCCCCACTCGTGTTGAAGCCCTTTCAGCCTTCATATGGAGCCGCTACATAGCATCATGCACCGGCATTCAACAAGATGGCGTCACCACCGACGCGATGTACACTGTTTCTCATGCTGTGAACCTCCGCACGAGAATGGAGCCACCACTTCCCGAGTACGCATTCGGAAACGTGACCCGAACTGCCATAGCGGTGCTGCCCACACCCGTTGACCCGAAAGATGTGACGCTTCATGGGGTGGTTAATCACGTCAGGGAAGCCATTAAGCAAGTAGACAAAGAGTTCATAAAGAAATTGCAAGAGGGTGACGGACACTTGAGCTTCTTGAGGAGCCGTACGGAGGAGGTGAAACGCGGAAAGGTGGTGTCGTTTAGCTTCACCAGTCTGTGTAGGTTTCCGATCTACGACGCTGATTTTGGATGGGGGAAGCCTGTTTATGTTGGTTCTGCCAGCTTTACGTTCAAGAACTTGGTTTGTTTCTTTGACACCAACGTAGGGGATGGAGTTGAGGCGTGGATCAATCTTTTGGAGGAAGACATGGAAAAATTCGAGGCAGACGAGGAGCTTCTGAAGTATGTTTCGCCCGCCCCGAGTTCAAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

440

Amino Acids

49.62

Weight (kDa)

6.4

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 428 2.2e-79 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
Acc36I ACCTGC 1 cut(s) 412
AccI GTMKAC 1 cut(s) 990
AccII CGCG 2 cut(s) 812, 1071
AccIII TCCGGA 1 cut(s) 602
AclWI GGATC 2 cut(s) 635, 1241
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 1 cut(s) 1262
AcuI CTGAAG 1 cut(s) 1307
AcyI GRCGYC 3 cut(s) 486, 718, 798
AfaI GTAC 5 cut(s) 299, 581, 818, 875, 1054
AfiI CCNNNNNNNGG 4 cut(s) 248, 753, 1057, 1212
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 6 cut(s) 11, 367, 734, 788, 1180, 1314
AjiI CACGTC 2 cut(s) 711, 967
AjnI CCWGG 2 cut(s) 162, 516
AluBI AGCT 8 cut(s) 82, 388, 398, 406, 1038, 1092, 1170, 1282
AluI AGCT 8 cut(s) 82, 388, 398, 406, 1038, 1092, 1170, 1282
Alw26I GTCTC 2 cut(s) 26, 574
AlwI GGATC 2 cut(s) 635, 1241
Ama87I CYCGRG 3 cut(s) 560, 869, 1306
Aor13HI TCCGGA 1 cut(s) 602
AoxI GGCC 4 cut(s) 309, 514, 519, 684
ApeKI GCWGC 3 cut(s) 195, 395, 913
ApoI RAATTY 1 cut(s) 1262
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
AspS9I GGNCC 2 cut(s) 127, 166
AsuC2I CCSGG 1 cut(s) 625
AsuHPI GGTGA 6 cut(s) 418, 545, 793, 1034, 1075, 1087
AvaI CYCGRG 3 cut(s) 560, 869, 1306
AvaII GGWCC 2 cut(s) 127, 166
BauI CACGAG 3 cut(s) 531, 726, 847
BbsI GAAGAC 2 cut(s) 655, 1257
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 3 cut(s) 207, 407, 900
BccI CCATC 5 cut(s) 185, 695, 788, 1131, 1211
BceAI ACGGC 1 cut(s) 1035
BcgI CGANNNNNNTGC 4 cut(s) 436, 470, 681, 715
BciT130I CCWGG 2 cut(s) 164, 518
BcnI CCSGG 1 cut(s) 625
BcoDI GTCTC 2 cut(s) 26, 574
BfaI CTAG 3 cut(s) 245, 389, 1321
BfuAI ACCTGC 1 cut(s) 412
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
BlsI GCNGC 7 cut(s) 197, 397, 443, 446, 523, 761, 915
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 3 cut(s) 164, 518, 625
Bme18I GGWCC 2 cut(s) 127, 166
BmeT110I CYCGRG 3 cut(s) 560, 869, 1306
BmgBI CACGTC 2 cut(s) 711, 967
BmgT120I GGNCC 2 cut(s) 127, 166
BmiI GGNNCC 3 cut(s) 758, 858, 1049
BmrFI CCNGG 3 cut(s) 164, 518, 625
BmsI GCATC 2 cut(s) 462, 779
BpiI GAAGAC 2 cut(s) 655, 1257
BplI GAGNNNNNCTC 4 cut(s) 8, 40, 554, 586
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 1054, 1063
BpuMI CCSGG 1 cut(s) 625
BsaHI GRCGYC 3 cut(s) 486, 718, 798
BsaJI CCNNGG 2 cut(s) 123, 459
BsaWI WCCGGW 1 cut(s) 602
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 476, 506
Bsc4I CCNNNNNNNGG 4 cut(s) 248, 753, 1057, 1212
Bse118I RCCGGY 1 cut(s) 779
Bse1I ACTGG 1 cut(s) 1098
BseAI TCCGGA 1 cut(s) 602
BseBI CCWGG 2 cut(s) 164, 518
BseDI CCNNGG 2 cut(s) 123, 459
BseGI GGATG 4 cut(s) 177, 687, 1142, 1222
BseLI CCNNNNNNNGG 4 cut(s) 248, 753, 1057, 1212
BseMII CTCAG 3 cut(s) 21, 516, 585
BseNI ACTGG 1 cut(s) 1098
BseRI GAGGAG 3 cut(s) 1060, 1073, 1292
BseXI GCAGC 3 cut(s) 207, 407, 900
Bsh1236I CGCG 2 cut(s) 812, 1071
BshFI GGCC 4 cut(s) 311, 516, 521, 686
BsiHKCI CYCGRG 3 cut(s) 560, 869, 1306
BsiSI CCGG 4 cut(s) 249, 603, 624, 780
BsiWI CGTACG 1 cut(s) 1052
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 4 cut(s) 248, 753, 1057, 1212
BsmAI GTCTC 2 cut(s) 26, 574
BsmBI CGTCTC 1 cut(s) 574
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 2 cut(s) 783, 878
BsnI GGCC 4 cut(s) 311, 516, 521, 686
BsoBI CYCGRG 3 cut(s) 560, 869, 1306
Bsp13I TCCGGA 1 cut(s) 602
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 3 cut(s) 627, 1116, 1233
Bsp19I CCATGG 2 cut(s) 123, 459
BspANI GGCC 4 cut(s) 311, 516, 521, 686
BspCNI CTCAG 3 cut(s) 22, 517, 584
BspEI TCCGGA 1 cut(s) 602
BspFNI CGCG 2 cut(s) 812, 1071
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 758, 858, 1049
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 2 cut(s) 635, 1241
BsrFI RCCGGY 1 cut(s) 779
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 1 cut(s) 1098
BssAI RCCGGY 1 cut(s) 779
BssECI CCNNGG 2 cut(s) 123, 459
BssMI GATC 3 cut(s) 627, 1116, 1233
BssNI GRCGYC 3 cut(s) 486, 718, 798
BssSI CACGAG 3 cut(s) 531, 726, 847
BssT1I CCWWGG 2 cut(s) 123, 459
Bst2BI CACGAG 3 cut(s) 531, 726, 847
Bst2UI CCWGG 2 cut(s) 164, 518
Bst4CI ACNGT 2 cut(s) 37, 823
Bst6I CTCTTC 2 cut(s) 137, 247
BstACI GRCGYC 3 cut(s) 486, 718, 798
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 4 cut(s) 419, 439, 1168, 1302
BstDEI CTNAG 5 cut(s) 30, 226, 357, 525, 571
BstDSI CCRYGG 2 cut(s) 123, 459
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 4 cut(s) 177, 687, 1142, 1222
BstFNI CGCG 2 cut(s) 812, 1071
BstKTI GATC 3 cut(s) 630, 1119, 1236
BstMAI GTCTC 2 cut(s) 26, 574
BstMBI GATC 3 cut(s) 627, 1116, 1233
BstMWI GCNNNNNNNGC 4 cut(s) 450, 527, 692, 743
BstNI CCWGG 2 cut(s) 164, 518
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 3 cut(s) 162, 516, 623
BstUI CGCG 2 cut(s) 812, 1071
BstV1I GCAGC 3 cut(s) 207, 407, 900
BstV2I GAAGAC 2 cut(s) 655, 1257
BsuRI GGCC 4 cut(s) 311, 516, 521, 686
BtgI CCRYGG 2 cut(s) 123, 459
BtgZI GCGATG 2 cut(s) 439, 827
BtrI CACGTC 2 cut(s) 711, 967
BtsCI GGATG 4 cut(s) 177, 687, 1142, 1222
BtsIMutI CAGTG 1 cut(s) 819
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 4 cut(s) 419, 439, 1168, 1302
CciI TCATGA 1 cut(s) 400
Cfr10I RCCGGY 1 cut(s) 779
Cfr13I GGNCC 2 cut(s) 127, 166
CseI GACGC 8 cut(s) 401, 494, 677, 726, 787, 818, 952, 1133
Csp6I GTAC 5 cut(s) 298, 580, 817, 874, 1053
CspCI CAANNNNNGTGG 4 cut(s) 712, 747, 907, 942
CviQI GTAC 5 cut(s) 298, 580, 817, 874, 1053
DdeI CTNAG 5 cut(s) 30, 226, 357, 525, 571
DpnI GATC 3 cut(s) 629, 1118, 1235
DpnII GATC 3 cut(s) 627, 1116, 1233
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 137, 247
EarI CTCTTC 2 cut(s) 137, 247
EciI GGCGGA 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 123, 459
Eco32I GATATC 1 cut(s) 468
Eco47I GGWCC 2 cut(s) 127, 166
Eco57I CTGAAG 1 cut(s) 1307
Eco88I CYCGRG 3 cut(s) 560, 869, 1306
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 2 cut(s) 162, 516
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 2 cut(s) 123, 459
ErhI CCWWGG 2 cut(s) 123, 459
Esp3I CGTCTC 1 cut(s) 574
FaqI GGGAC 1 cut(s) 497
FauI CCCGC 1 cut(s) 1309
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
FokI GGATG 4 cut(s) 164, 674, 1149, 1229
Fsp4HI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
FspBI CTAG 3 cut(s) 245, 389, 1321
GluI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
HaeIII GGCC 4 cut(s) 311, 516, 521, 686
HapII CCGG 4 cut(s) 249, 603, 624, 780
HgaI GACGC 8 cut(s) 401, 494, 677, 726, 787, 818, 952, 1133
Hin1I GRCGYC 3 cut(s) 486, 718, 798
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 2 cut(s) 255, 606
HpaII CCGG 4 cut(s) 249, 603, 624, 780
HphI GGTGA 6 cut(s) 418, 545, 793, 1034, 1075, 1087
Hpy166II GTNNAC 5 cut(s) 259, 819, 838, 928, 991
Hpy188I TCNGA 7 cut(s) 220, 334, 574, 692, 884, 1116, 1287
Hpy188III TCNNGA 8 cut(s) 205, 401, 560, 603, 714, 869, 1042, 1180
Hpy8I GTNNAC 5 cut(s) 259, 819, 838, 928, 991
Hpy99I CGWCG 5 cut(s) 290, 308, 671, 812, 1127
HpyAV CCTTC 6 cut(s) 202, 284, 386, 439, 532, 757
HpyCH4III ACNGT 2 cut(s) 37, 823
HpyCH4IV ACGT 8 cut(s) 303, 498, 503, 710, 888, 966, 1175, 1209
HpyCH4V TGCA 6 cut(s) 195, 282, 324, 421, 777, 1015
HpyF10VI GCNNNNNNNGC 4 cut(s) 450, 527, 692, 743
HpyF3I CTNAG 5 cut(s) 30, 226, 357, 525, 571
HpySE526I ACGT 8 cut(s) 303, 498, 503, 710, 888, 966, 1175, 1209
Hsp92I GRCGYC 3 cut(s) 486, 718, 798
Kpn2I TCCGGA 1 cut(s) 602
Kzo9I GATC 3 cut(s) 627, 1116, 1233
LmnI GCTCC 6 cut(s) 293, 676, 756, 856, 1047, 1279
Lsp1109I GCAGC 3 cut(s) 207, 407, 900
LweI GCATC 2 cut(s) 462, 779
MaeI CTAG 3 cut(s) 245, 389, 1321
MaeII ACGT 8 cut(s) 303, 498, 503, 710, 888, 966, 1175, 1209
MaeIII GTNAC 6 cut(s) 424, 499, 799, 889, 940, 1022
MalI GATC 3 cut(s) 629, 1118, 1235
MboI GATC 3 cut(s) 627, 1116, 1233
MboII GAAGA 7 cut(s) 106, 124, 264, 424, 601, 655, 1262
MluCI AATT 2 cut(s) 1010, 1262
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 3 cut(s) 77, 243, 637
MroI TCCGGA 1 cut(s) 602
MseI TTAA 2 cut(s) 960, 981
MslI CAYNNNNRTG 4 cut(s) 122, 458, 851, 908
MspA1I CMGCKG 1 cut(s) 524
MspI CCGG 4 cut(s) 249, 603, 624, 780
MspR9I CCNGG 3 cut(s) 164, 518, 625
Mva1269I GAATGC 2 cut(s) 783, 878
MvaI CCWGG 2 cut(s) 164, 518
MvnI CGCG 2 cut(s) 812, 1071
MwoI GCNNNNNNNGC 4 cut(s) 450, 527, 692, 743
NciI CCSGG 1 cut(s) 625
NcoI CCATGG 2 cut(s) 123, 459
NdeI CATATG 1 cut(s) 752
NdeII GATC 3 cut(s) 627, 1116, 1233
NlaIV GGNNCC 3 cut(s) 758, 858, 1049
NmuCI GTSAC 6 cut(s) 424, 499, 799, 889, 940, 1022
PagI TCATGA 1 cut(s) 400
PcsI WCGNNNNNNNCGW 4 cut(s) 282, 724, 1272, 1304
PctI GAATGC 2 cut(s) 783, 878
PfeI GAWTC 1 cut(s) 606
Pfl23II CGTACG 1 cut(s) 1052
PkrI GCNGC 7 cut(s) 197, 397, 443, 446, 523, 761, 915
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 162, 516
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 2 cut(s) 162, 516
PspLI CGTACG 1 cut(s) 1052
PspN4I GGNNCC 3 cut(s) 758, 858, 1049
PspPI GGNCC 2 cut(s) 127, 166
RsaI GTAC 5 cut(s) 299, 581, 818, 875, 1054
RsaNI GTAC 5 cut(s) 298, 580, 817, 874, 1053
RseI CAYNNNNRTG 4 cut(s) 122, 458, 851, 908
SaqAI TTAA 2 cut(s) 960, 981
SatI GCNGC 7 cut(s) 196, 396, 442, 445, 522, 760, 914
Sau3AI GATC 3 cut(s) 627, 1116, 1233
Sau96I GGNCC 2 cut(s) 127, 166
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 3 cut(s) 164, 518, 625
SfaNI GCATC 2 cut(s) 462, 779
SinI GGWCC 2 cut(s) 127, 166
SmiMI CAYNNNNRTG 4 cut(s) 122, 458, 851, 908
SmlI CTYRAG 2 cut(s) 1033, 1042
SmoI CTYRAG 2 cut(s) 1033, 1042
Sse9I AATT 2 cut(s) 1010, 1262
SspMI CTAG 3 cut(s) 245, 389, 1321
StyD4I CCNGG 3 cut(s) 162, 516, 623
StyI CCWWGG 2 cut(s) 123, 459
TaaI ACNGT 2 cut(s) 37, 823
TaiI ACGT 8 cut(s) 306, 501, 506, 713, 891, 969, 1178, 1212
TaqI TCGA 7 cut(s) 93, 276, 306, 342, 384, 666, 1266
TaqII GACCGA 1 cut(s) 332
TasI AATT 2 cut(s) 1010, 1262
TatI WGTACW 2 cut(s) 579, 816
TauI GCSGC 4 cut(s) 444, 447, 524, 762
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 2 cut(s) 960, 981
Tru9I TTAA 2 cut(s) 960, 981
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 6 cut(s) 424, 499, 799, 889, 940, 1022
TseI GCWGC 3 cut(s) 195, 395, 913
Tsp45I GTSAC 6 cut(s) 424, 499, 799, 889, 940, 1022
TspDTI ATGAA 6 cut(s) 17, 106, 198, 739, 938, 991
TspGWI ACGGA 2 cut(s) 1041, 1070
TspRI CASTG 1 cut(s) 826
VpaK11BI GGWCC 2 cut(s) 127, 166
XapI RAATTY 1 cut(s) 1262
XmiI GTMKAC 1 cut(s) 990
XspI CTAG 3 cut(s) 245, 389, 1321
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.