FvH4_4g24800

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000852
Physical Location & Seq
Reverse (-)
26811837 .. 26813180
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24800.t1

Sequence Viewer

Length: 1344 bp
ATGAAGTTTCAAGTGGAGGTACTCTCAACAGAGACAATCAAACCCTCGTCTCCGACCCCTGACCACCTCCGCCACCACAAGCTCTCCTATCTCGATCAAATCCAGCCTCCAATCTTCATGCCCATGGTCCTCTTCTACCCCAAAGAGCACGACCACAACGACCTCGACCTCATTTCCATCCACGAACAGCGTTGCAGCAAAATAAAGAAGGCCTTATCCGACACCCTAGTCAAGTTCTACCCACTAGCCGGAAGAGTCCACGGAACCCAATACGTCGATTGCAACGACGAAGGAGCCTACTACGTCGAAGCCAAGACCGACTGCAAATTATCCGACATCATCGAAAACCCAAACCCTAAAGACTTCAACAAGTTCCTCCCCTTCGAGCTCGACGCGGCTCATGAGCTTCCTGTGTGCTTCCAGGTCACCTTCTTCGCCTGCGGCGGCATGAGCATCGCCATGGGAATGTCCCACAAGGTCGGGGATGCCCTCTCCTACTTCACTTTCCTCAACGGCCTCGCCGCTGAGGCTCGCGGGGAGGGCAACGATGTTTGCGCTCCGGTGTTCGTCTCGGACAAGTACTTCCCCCAGAAGGACTTGTCCGGATTCTACCAACACCGCAGCGGGATCATCAAGCAAAACATCTCCACAAAACGCTTCGTCTTTGACGGTCCAATGGTTCAGGCCATCCGAGCCAAATGCGTTGACATGTCCAGACGTCCTACTCGCGTTGAAGCCTTATCAGCCTTCATATGGTGCCGCTACATAGCATCATGCACCAGCATTCAACAAGAGGGCGTCACCACCGACGCCATGTACACTGTTTCTCACGCCGTGAACCTCCGCACGAGAATGGAGCCTCCACTTCCGGAGCACACATTCGGAAACGTGACCCGAACCGCCATCGCCGTGCCGCCGGTACCCGTTGACCCGAAAGACGGGACGCTTCATGGGGTGGTTAATCACGTAAGGGAGGCCATCAAGCAAGTCGACAAAGAGTACATAAGAAAACTGCAAGAGGGTGACGCACACTTGAACTTCTTGAGGAGCCGCACTGAAGAGGTCAAACGCGGAGAGGTGGTGTCGTTTAGCTTCACGAGCCTGTGTAGGTTTCCAATCTACGACGCTGATTTCGGGTGGGGGAAGCCTGTGTATGTCGGTTCTGCTAGCTTTACGTTCAAGAACTTGGTTAGTTTCTTTGACACCAACGTAGGGGATGGAGTTGAGGCGTGGATCAATCTGTTGGAGGAAGACATGGAAAAGTTCGAGAAGGACGAGGAGCTTCTCAAGTATGTTTCACTGGCCCCGAGTTACAAAAATGCCAAGGTCTCCATAAATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

448

Amino Acids

50.58

Weight (kDa)

6.12

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 428 1.7e-81 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AatII GACGTC 1 cut(s) 721
Acc65I GGTACC 1 cut(s) 919
AccB1I GGYRCC 2 cut(s) 756, 919
AccI GTMKAC 1 cut(s) 990
AccII CGCG 4 cut(s) 395, 534, 729, 1071
AccIII TCCGGA 2 cut(s) 602, 868
AclWI GGATC 2 cut(s) 635, 1241
AcuI CTGAAG 1 cut(s) 1077
AcyI GRCGYC 3 cut(s) 718, 798, 810
AdeI CACNNNGTG 1 cut(s) 835
AfaI GTAC 5 cut(s) 21, 581, 818, 921, 1001
AfiI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1212
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 6 cut(s) 11, 367, 734, 788, 1036, 1180
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 6 cut(s) 82, 388, 406, 1092, 1170, 1282
AluI AGCT 6 cut(s) 82, 388, 406, 1092, 1170, 1282
Alw21I GWGCWC 3 cut(s) 150, 390, 876
Alw26I GTCTC 4 cut(s) 26, 54, 574, 1333
AlwI GGATC 2 cut(s) 635, 1241
Ama87I CYCGRG 1 cut(s) 1306
Aor13HI TCCGGA 2 cut(s) 602, 868
AoxI GGCC 5 cut(s) 210, 514, 684, 975, 1302
ApeKI GCWGC 2 cut(s) 195, 621
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
Asp718I GGTACC 1 cut(s) 919
AspLEI GCGC 1 cut(s) 557
AspS9I GGNCC 3 cut(s) 127, 671, 1303
AsuHPI GGTGA 3 cut(s) 418, 793, 1034
AsuNHI GCTAGC 1 cut(s) 1166
AvaI CYCGRG 1 cut(s) 1306
AvaII GGWCC 2 cut(s) 127, 671
BanI GGYRCC 2 cut(s) 756, 919
BanII GRGCYC 1 cut(s) 390
BauI CACGAG 2 cut(s) 847, 1096
BbsI GAAGAC 1 cut(s) 1257
Bbv12I GWGCWC 3 cut(s) 150, 390, 876
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 207, 633
BccI CCATC 5 cut(s) 185, 695, 911, 986, 1211
BceAI ACGGC 3 cut(s) 529, 818, 893
BcgI CGANNNNNNTGC 4 cut(s) 436, 470, 681, 715
BciT130I CCWGG 1 cut(s) 422
BcoDI GTCTC 4 cut(s) 26, 54, 574, 1333
BfaI CTAG 3 cut(s) 227, 245, 1167
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 9 cut(s) 196, 396, 442, 445, 522, 622, 760, 914, 1051
BlsI GCNGC 9 cut(s) 197, 397, 443, 446, 523, 623, 761, 915, 1052
BmcAI AGTACT 1 cut(s) 581
Bme1390I CCNGG 1 cut(s) 422
Bme18I GGWCC 2 cut(s) 127, 671
BmeT110I CYCGRG 1 cut(s) 1306
BmgT120I GGNCC 3 cut(s) 127, 671, 1303
BmiI GGNNCC 7 cut(s) 265, 295, 758, 858, 921, 1049, 1305
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 3 cut(s) 462, 475, 779
BmtI GCTAGC 1 cut(s) 1170
BpiI GAAGAC 1 cut(s) 1257
BplI GAGNNNNNCTC 2 cut(s) 8, 40
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 1063, 1271
BsaAI YACGTR 1 cut(s) 967
BsaHI GRCGYC 3 cut(s) 718, 798, 810
BsaI GGTCTC 1 cut(s) 1333
BsaJI CCNNGG 4 cut(s) 123, 259, 459, 1323
BsaWI WCCGGW 3 cut(s) 559, 602, 868
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1212
Bse118I RCCGGY 1 cut(s) 916
Bse1I ACTGG 1 cut(s) 1305
BseAI TCCGGA 2 cut(s) 602, 868
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 4 cut(s) 123, 259, 459, 1323
BseGI GGATG 4 cut(s) 177, 490, 687, 1222
BseLI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1212
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 1 cut(s) 1305
BseRI GAGGAG 2 cut(s) 1060, 1292
BseXI GCAGC 2 cut(s) 207, 633
Bsh1236I CGCG 4 cut(s) 395, 534, 729, 1071
BshFI GGCC 5 cut(s) 212, 516, 686, 977, 1304
BshNI GGYRCC 2 cut(s) 756, 919
BsiHKAI GWGCWC 3 cut(s) 150, 390, 876
BsiHKCI CYCGRG 1 cut(s) 1306
BsiSI CCGG 5 cut(s) 249, 560, 603, 869, 917
BslFI GGGAC 2 cut(s) 454, 955
BslI CCNNNNNNNGG 5 cut(s) 248, 592, 753, 938, 1212
BsmAI GTCTC 4 cut(s) 26, 54, 574, 1333
BsmBI CGTCTC 2 cut(s) 54, 574
BsmFI GGGAC 2 cut(s) 454, 955
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 5 cut(s) 212, 516, 686, 977, 1304
Bso31I GGTCTC 1 cut(s) 1333
BsoBI CYCGRG 1 cut(s) 1306
Bsp1286I GDGCHC 3 cut(s) 150, 390, 876
Bsp13I TCCGGA 2 cut(s) 602, 868
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 3 cut(s) 94, 627, 1233
Bsp19I CCATGG 2 cut(s) 123, 459
BspANI GGCC 5 cut(s) 212, 516, 686, 977, 1304
BspCNI CTCAG 1 cut(s) 517
BspEI TCCGGA 2 cut(s) 602, 868
BspFNI CGCG 4 cut(s) 395, 534, 729, 1071
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 7 cut(s) 265, 295, 758, 858, 921, 1049, 1305
BspOI GCTAGC 1 cut(s) 1170
BspPI GGATC 2 cut(s) 635, 1241
BspT107I GGYRCC 2 cut(s) 756, 919
BspTNI GGTCTC 1 cut(s) 1333
BsrFI RCCGGY 1 cut(s) 916
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 1 cut(s) 1305
BssAI RCCGGY 1 cut(s) 916
BssECI CCNNGG 4 cut(s) 123, 259, 459, 1323
BssMI GATC 3 cut(s) 94, 627, 1233
BssNI GRCGYC 3 cut(s) 718, 798, 810
BssSI CACGAG 2 cut(s) 847, 1096
BssT1I CCWWGG 3 cut(s) 123, 459, 1323
Bst2BI CACGAG 2 cut(s) 847, 1096
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 671, 823
Bst6I CTCTTC 3 cut(s) 137, 247, 1053
BstACI GRCGYC 3 cut(s) 718, 798, 810
BstAUI TGTACA 1 cut(s) 816
BstBAI YACGTR 1 cut(s) 967
BstC8I GCNNGC 3 cut(s) 439, 532, 1168
BstDEI CTNAG 1 cut(s) 525
BstDSI CCRYGG 3 cut(s) 123, 259, 459
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 4 cut(s) 177, 490, 687, 1222
BstFNI CGCG 4 cut(s) 395, 534, 729, 1071
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 3 cut(s) 97, 630, 1236
BstMAI GTCTC 4 cut(s) 26, 54, 574, 1333
BstMBI GATC 3 cut(s) 94, 627, 1233
BstMWI GCNNNNNNNGC 6 cut(s) 450, 527, 540, 692, 743, 1098
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 712
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 420
BstUI CGCG 4 cut(s) 395, 534, 729, 1071
BstV1I GCAGC 2 cut(s) 207, 633
BstV2I GAAGAC 1 cut(s) 1257
BsuRI GGCC 5 cut(s) 212, 516, 686, 977, 1304
BtgI CCRYGG 3 cut(s) 123, 259, 459
BtgZI GCGATG 2 cut(s) 439, 889
BtsCI GGATG 4 cut(s) 177, 490, 687, 1222
BtsIMutI CAGTG 3 cut(s) 819, 1053, 1298
Cac8I GCNNGC 3 cut(s) 439, 532, 1168
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 557
Cfr10I RCCGGY 1 cut(s) 916
Cfr13I GGNCC 3 cut(s) 127, 671, 1303
CseI GACGC 6 cut(s) 401, 787, 818, 952, 1034, 1133
Csp6I GTAC 5 cut(s) 20, 580, 817, 920, 1000
CviQI GTAC 5 cut(s) 20, 580, 817, 920, 1000
DdeI CTNAG 1 cut(s) 525
DpnI GATC 3 cut(s) 96, 629, 1235
DpnII GATC 3 cut(s) 94, 627, 1233
DraIII CACNNNGTG 1 cut(s) 835
DrdI GACNNNNNNGTC 1 cut(s) 227
DseDI GACNNNNNNGTC 1 cut(s) 227
Eam1104I CTCTTC 3 cut(s) 137, 247, 1053
EarI CTCTTC 3 cut(s) 137, 247, 1053
EciI GGCGGA 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 388
Eco130I CCWWGG 3 cut(s) 123, 459, 1323
Eco147I AGGCCT 1 cut(s) 212
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 1333
Eco47I GGWCC 2 cut(s) 127, 671
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 1077
Eco88I CYCGRG 1 cut(s) 1306
Eco91I GGTNACC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 3 cut(s) 123, 459, 1323
EcoT38I GRGCYC 1 cut(s) 390
ErhI CCWWGG 3 cut(s) 123, 459, 1323
Esp3I CGTCTC 2 cut(s) 54, 574
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 2 cut(s) 454, 955
FauI CCCGC 2 cut(s) 527, 617
FauNDI CATATG 1 cut(s) 752
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 9 cut(s) 196, 396, 442, 445, 522, 622, 760, 914, 1051
FokI GGATG 4 cut(s) 164, 497, 674, 1229
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 9 cut(s) 196, 396, 442, 445, 522, 622, 760, 914, 1051
FspBI CTAG 3 cut(s) 227, 245, 1167
GlaI GCGC 1 cut(s) 556
GluI GCNGC 9 cut(s) 196, 396, 442, 445, 522, 622, 760, 914, 1051
HaeIII GGCC 5 cut(s) 212, 516, 686, 977, 1304
HapII CCGG 5 cut(s) 249, 560, 603, 869, 917
HgaI GACGC 6 cut(s) 401, 787, 818, 952, 1034, 1133
HhaI GCGC 1 cut(s) 557
Hin1I GRCGYC 3 cut(s) 718, 798, 810
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HincII GTYRAC 3 cut(s) 706, 928, 991
HindII GTYRAC 3 cut(s) 706, 928, 991
HinfI GANTC 2 cut(s) 255, 606
HpaII CCGG 5 cut(s) 249, 560, 603, 869, 917
HphI GGTGA 3 cut(s) 418, 793, 1034
Hpy166II GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy188I TCNGA 6 cut(s) 54, 220, 334, 574, 692, 884
Hpy188III TCNNGA 9 cut(s) 92, 401, 603, 714, 869, 1042, 1096, 1180, 1267
Hpy8I GTNNAC 6 cut(s) 259, 706, 819, 838, 928, 991
Hpy99I CGWCG 6 cut(s) 278, 290, 308, 395, 812, 1127
HpyAV CCTTC 7 cut(s) 202, 284, 391, 439, 586, 757, 1264
HpyCH4III ACNGT 2 cut(s) 671, 823
HpyCH4IV ACGT 7 cut(s) 273, 303, 718, 888, 966, 1175, 1209
HpyCH4V TGCA 5 cut(s) 195, 282, 324, 777, 1015
HpyF10VI GCNNNNNNNGC 6 cut(s) 450, 527, 540, 692, 743, 1098
HpyF3I CTNAG 1 cut(s) 525
HpySE526I ACGT 7 cut(s) 273, 303, 718, 888, 966, 1175, 1209
Hsp92I GRCGYC 3 cut(s) 718, 798, 810
HspAI GCGC 1 cut(s) 555
Kpn2I TCCGGA 2 cut(s) 602, 868
KpnI GGTACC 1 cut(s) 923
Kzo9I GATC 3 cut(s) 94, 627, 1233
LmnI GCTCC 6 cut(s) 293, 562, 856, 871, 1047, 1279
Lsp1109I GCAGC 2 cut(s) 207, 633
LweI GCATC 3 cut(s) 462, 475, 779
MaeI CTAG 3 cut(s) 227, 245, 1167
MaeII ACGT 7 cut(s) 273, 303, 718, 888, 966, 1175, 1209
MaeIII GTNAC 5 cut(s) 424, 799, 889, 1022, 1310
MalI GATC 3 cut(s) 96, 629, 1235
MboI GATC 3 cut(s) 94, 627, 1233
MboII GAAGA 6 cut(s) 106, 124, 264, 424, 1070, 1262
MhlI GDGCHC 3 cut(s) 150, 390, 876
MluCI AATT 2 cut(s) 326, 1336
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 4 cut(s) 77, 243, 357, 1224
MroI TCCGGA 2 cut(s) 602, 868
MseI TTAA 1 cut(s) 960
MslI CAYNNNNRTG 5 cut(s) 122, 458, 464, 851, 908
MspA1I CMGCKG 2 cut(s) 524, 624
MspI CCGG 5 cut(s) 249, 560, 603, 869, 917
MspR9I CCNGG 1 cut(s) 422
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 1 cut(s) 422
MvnI CGCG 4 cut(s) 395, 534, 729, 1071
MwoI GCNNNNNNNGC 6 cut(s) 450, 527, 540, 692, 743, 1098
NcoI CCATGG 2 cut(s) 123, 459
NdeI CATATG 1 cut(s) 752
NdeII GATC 3 cut(s) 94, 627, 1233
NheI GCTAGC 1 cut(s) 1166
NlaIV GGNNCC 7 cut(s) 265, 295, 758, 858, 921, 1049, 1305
NmuCI GTSAC 4 cut(s) 424, 799, 889, 1022
NspI RCATGY 1 cut(s) 712
PagI TCATGA 1 cut(s) 400
PceI AGGCCT 1 cut(s) 212
PciI ACATGT 1 cut(s) 708
PcsI WCGNNNNNNNCGW 3 cut(s) 156, 282, 1272
PctI GAATGC 1 cut(s) 783
PfeI GAWTC 1 cut(s) 606
PkrI GCNGC 9 cut(s) 197, 397, 443, 446, 523, 623, 761, 915, 1052
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Ppu21I YACGTR 1 cut(s) 967
PscI ACATGT 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 420
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 7 cut(s) 265, 295, 758, 858, 921, 1049, 1305
PspPI GGNCC 3 cut(s) 127, 671, 1303
RsaI GTAC 5 cut(s) 21, 581, 818, 921, 1001
RsaNI GTAC 5 cut(s) 20, 580, 817, 920, 1000
RseI CAYNNNNRTG 5 cut(s) 122, 458, 464, 851, 908
SacI GAGCTC 1 cut(s) 390
SalI GTCGAC 1 cut(s) 989
SaqAI TTAA 1 cut(s) 960
SatI GCNGC 9 cut(s) 196, 396, 442, 445, 522, 622, 760, 914, 1051
Sau3AI GATC 3 cut(s) 94, 627, 1233
Sau96I GGNCC 3 cut(s) 127, 671, 1303
ScaI AGTACT 1 cut(s) 581
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 3 cut(s) 150, 390, 876
SfaNI GCATC 3 cut(s) 462, 475, 779
SinI GGWCC 2 cut(s) 127, 671
SmiMI CAYNNNNRTG 5 cut(s) 122, 458, 464, 851, 908
SmlI CTYRAG 2 cut(s) 1042, 1286
SmoI CTYRAG 2 cut(s) 1042, 1286
Sse9I AATT 2 cut(s) 326, 1336
SseBI AGGCCT 1 cut(s) 212
SspMI CTAG 3 cut(s) 227, 245, 1167
SstI GAGCTC 1 cut(s) 390
StuI AGGCCT 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 3 cut(s) 123, 459, 1323
TaaI ACNGT 2 cut(s) 671, 823
TaiI ACGT 7 cut(s) 276, 306, 721, 891, 969, 1178, 1212
TaqI TCGA 9 cut(s) 93, 165, 276, 306, 342, 384, 390, 990, 1266
TaqII GACCGA 1 cut(s) 332
TasI AATT 2 cut(s) 326, 1336
TatI WGTACW 3 cut(s) 579, 816, 999
TauI GCSGC 7 cut(s) 398, 444, 447, 524, 762, 916, 1053
TfiI GAWTC 1 cut(s) 606
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 3 cut(s) 826, 1060, 1305
TseFI GTSAC 4 cut(s) 424, 799, 889, 1022
TseI GCWGC 2 cut(s) 195, 621
Tsp45I GTSAC 4 cut(s) 424, 799, 889, 1022
TspDTI ATGAA 4 cut(s) 17, 106, 739, 938
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 3 cut(s) 826, 1060, 1305
VpaK11BI GGWCC 2 cut(s) 127, 671
XceI RCATGY 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 990
XspI CTAG 3 cut(s) 227, 245, 1167
ZraI GACGTC 1 cut(s) 719
ZrmI AGTACT 1 cut(s) 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.