Rmu_sc0004487.1_g000007

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004487.1
OG0000852
Physical Location & Seq
Reverse (-)
21834 .. 22481
648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004487.1_g000007.1.cds

Sequence Viewer

Length: 648 bp
atggttcaggccatccgagccaaatgcatggacatgtccagacgccccactcgtgttgaagccctttcagccttcatatggagccgctacatagcatcatgcaccgacattcaacaagatagcgtcaccaccgacgcgatgtacactgtttctcatactatgaacctccgcacgagaatggagccaccacttcccgagtacgcattcgggaacgtgacccgaaccgccattgcagtgccgcccatacccgttgacccgaaagatgggacgcttcatggggtggttaattacgtcaaggaggccattaagcaagtagacaaagagttcataaagaaattgcaagagggtgacggacacttgagcttcttgaggagccgtacggaggaggtgaaacgcggagaggtggtgtcgtttagcttcacaagtttgtgtaggtttccgatctacgacgctgatttgggatgggggaagcctattaatgttggttctgccagctttacgttcaagaacttggttagtttctttgacaccaacgtaggggatggagttgaggcgtggatcaatcttttggaggaagacatggaaaaattcgaggcagacgaggagcttctgaagtatgtttcacccgccccgagttcaaagaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

215

Amino Acids

24.2

Weight (kDa)

5.33

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 315
AccII CGCG 2 cut(s) 137, 396
AciI CCGC 6 cut(s) 85, 169, 225, 239, 396, 627
AclWI GGATC 1 cut(s) 566
AcsI RAATTY 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 43
AfaI GTAC 3 cut(s) 143, 200, 379
AfiI CCNNNNNNNGG 4 cut(s) 78, 263, 382, 537
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 4 cut(s) 59, 113, 505, 639
AluBI AGCT 4 cut(s) 363, 417, 495, 607
AluI AGCT 4 cut(s) 363, 417, 495, 607
AlwI GGATC 1 cut(s) 566
Ama87I CYCGRG 2 cut(s) 194, 631
AoxI GGCC 2 cut(s) 9, 300
ApoI RAATTY 1 cut(s) 587
AseI ATTAAT 1 cut(s) 477
AsuHPI GGTGA 4 cut(s) 118, 359, 400, 615
AvaI CYCGRG 2 cut(s) 194, 631
BauI CACGAG 2 cut(s) 51, 172
BbsI GAAGAC 1 cut(s) 582
BccI CCATC 4 cut(s) 20, 257, 456, 536
BceAI ACGGC 1 cut(s) 360
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BfaI CTAG 1 cut(s) 646
BisI GCNGC 2 cut(s) 85, 239
BlsI GCNGC 2 cut(s) 86, 240
BmeT110I CYCGRG 2 cut(s) 194, 631
BmiI GGNNCC 3 cut(s) 83, 183, 374
BmsI GCATC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 379, 388
BsaHI GRCGYC 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 263, 382, 537
Bse3DI GCAATG 1 cut(s) 228
BseGI GGATG 3 cut(s) 12, 467, 547
BseLI CCNNNNNNNGG 4 cut(s) 78, 263, 382, 537
BseMI GCAATG 1 cut(s) 228
BseRI GAGGAG 3 cut(s) 385, 398, 617
Bsh1236I CGCG 2 cut(s) 137, 396
BshFI GGCC 2 cut(s) 11, 302
BsiHKCI CYCGRG 2 cut(s) 194, 631
BsiWI CGTACG 1 cut(s) 377
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 4 cut(s) 78, 263, 382, 537
BsmFI GGGAC 1 cut(s) 280
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 2 cut(s) 11, 302
BsoBI CYCGRG 2 cut(s) 194, 631
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 2 cut(s) 441, 558
BspACI CCGC 6 cut(s) 85, 169, 225, 239, 396, 627
BspANI GGCC 2 cut(s) 11, 302
BspFNI CGCG 2 cut(s) 137, 396
BspLI GGNNCC 3 cut(s) 83, 183, 374
BspPI GGATC 1 cut(s) 566
BsrDI GCAATG 1 cut(s) 228
BsrGI TGTACA 1 cut(s) 141
BssMI GATC 2 cut(s) 441, 558
BssNI GRCGYC 1 cut(s) 43
BssSI CACGAG 2 cut(s) 51, 172
Bst2BI CACGAG 2 cut(s) 51, 172
Bst4CI ACNGT 1 cut(s) 148
BstACI GRCGYC 1 cut(s) 43
BstAUI TGTACA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 493
BstF5I GGATG 3 cut(s) 12, 467, 547
BstFNI CGCG 2 cut(s) 137, 396
BstKTI GATC 2 cut(s) 444, 561
BstMBI GATC 2 cut(s) 441, 558
BstMWI GCNNNNNNNGC 2 cut(s) 17, 68
BstNSI RCATGY 1 cut(s) 37
BstUI CGCG 2 cut(s) 137, 396
BstV2I GAAGAC 1 cut(s) 582
BstXI CCANNNNNNTGG 1 cut(s) 28
BsuRI GGCC 2 cut(s) 11, 302
BtgZI GCGATG 1 cut(s) 152
BtsCI GGATG 3 cut(s) 12, 467, 547
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 2 cut(s) 144, 240
Cac8I GCNNGC 1 cut(s) 493
CseI GACGC 5 cut(s) 51, 112, 143, 277, 458
Csp6I GTAC 3 cut(s) 142, 199, 378
CspCI CAANNNNNGTGG 2 cut(s) 37, 72
CviAII CATG 5 cut(s) 28, 34, 99, 275, 580
CviQI GTAC 3 cut(s) 142, 199, 378
DpnI GATC 2 cut(s) 443, 560
DpnII GATC 2 cut(s) 441, 558
Eco57I CTGAAG 1 cut(s) 632
Eco88I CYCGRG 2 cut(s) 194, 631
EcoT22I ATGCAT 1 cut(s) 29
FaeI CATG 5 cut(s) 31, 37, 102, 278, 583
FaqI GGGAC 1 cut(s) 280
FatI CATG 5 cut(s) 27, 33, 98, 274, 579
FauI CCCGC 1 cut(s) 634
FauNDI CATATG 1 cut(s) 77
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 2 cut(s) 85, 239
FokI GGATG 2 cut(s) 474, 554
Fsp4HI GCNGC 2 cut(s) 85, 239
FspBI CTAG 1 cut(s) 646
GluI GCNGC 2 cut(s) 85, 239
HaeIII GGCC 2 cut(s) 11, 302
HgaI GACGC 5 cut(s) 51, 112, 143, 277, 458
Hin1I GRCGYC 1 cut(s) 43
Hin1II CATG 5 cut(s) 31, 37, 102, 278, 583
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HphI GGTGA 4 cut(s) 118, 359, 400, 615
Hpy166II GTNNAC 3 cut(s) 144, 253, 316
Hpy188I TCNGA 3 cut(s) 17, 441, 612
Hpy188III TCNNGA 5 cut(s) 39, 194, 208, 367, 505
Hpy8I GTNNAC 3 cut(s) 144, 253, 316
Hpy99I CGWCG 2 cut(s) 137, 452
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 4 cut(s) 213, 291, 500, 534
HpyCH4V TGCA 4 cut(s) 27, 102, 233, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 68
HpySE526I ACGT 4 cut(s) 213, 291, 500, 534
Hsp92I GRCGYC 1 cut(s) 43
Hsp92II CATG 5 cut(s) 31, 37, 102, 278, 583
Kzo9I GATC 2 cut(s) 441, 558
LmnI GCTCC 4 cut(s) 81, 181, 372, 604
LpnPI CCDG 2 cut(s) 52, 505
LweI GCATC 1 cut(s) 104
MaeI CTAG 1 cut(s) 646
MaeII ACGT 4 cut(s) 213, 291, 500, 534
MaeIII GTNAC 3 cut(s) 124, 214, 347
MalI GATC 2 cut(s) 443, 560
MboI GATC 2 cut(s) 441, 558
MboII GAAGA 1 cut(s) 587
MluCI AATT 3 cut(s) 286, 335, 587
Mph1103I ATGCAT 1 cut(s) 29
MseI TTAA 3 cut(s) 285, 306, 477
MslI CAYNNNNRTG 3 cut(s) 32, 176, 233
Mva1269I GAATGC 1 cut(s) 203
MvnI CGCG 2 cut(s) 137, 396
MwoI GCNNNNNNNGC 2 cut(s) 17, 68
NdeI CATATG 1 cut(s) 77
NdeII GATC 2 cut(s) 441, 558
NlaIII CATG 5 cut(s) 31, 37, 102, 278, 583
NlaIV GGNNCC 3 cut(s) 83, 183, 374
NmuCI GTSAC 3 cut(s) 124, 214, 347
NsiI ATGCAT 1 cut(s) 29
NspI RCATGY 1 cut(s) 37
PciI ACATGT 1 cut(s) 33
PcsI WCGNNNNNNNCGW 2 cut(s) 49, 597
PctI GAATGC 1 cut(s) 203
Pfl23II CGTACG 1 cut(s) 377
PkrI GCNGC 2 cut(s) 86, 240
PscI ACATGT 1 cut(s) 33
PshBI ATTAAT 1 cut(s) 477
PspLI CGTACG 1 cut(s) 377
PspN4I GGNNCC 3 cut(s) 83, 183, 374
RsaI GTAC 3 cut(s) 143, 200, 379
RsaNI GTAC 3 cut(s) 142, 199, 378
RseI CAYNNNNRTG 3 cut(s) 32, 176, 233
SaqAI TTAA 3 cut(s) 285, 306, 477
SatI GCNGC 2 cut(s) 85, 239
Sau3AI GATC 2 cut(s) 441, 558
SfaNI GCATC 1 cut(s) 104
SmiMI CAYNNNNRTG 3 cut(s) 32, 176, 233
SmlI CTYRAG 2 cut(s) 358, 367
SmoI CTYRAG 2 cut(s) 358, 367
Sse9I AATT 3 cut(s) 286, 335, 587
SsiI CCGC 6 cut(s) 85, 169, 225, 239, 396, 627
SspMI CTAG 1 cut(s) 646
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 4 cut(s) 216, 294, 503, 537
TaqI TCGA 1 cut(s) 591
TasI AATT 3 cut(s) 286, 335, 587
TatI WGTACW 1 cut(s) 141
TauI GCSGC 2 cut(s) 87, 241
Tru1I TTAA 3 cut(s) 285, 306, 477
Tru9I TTAA 3 cut(s) 285, 306, 477
TscAI CASTG 2 cut(s) 151, 240
TseFI GTSAC 3 cut(s) 124, 214, 347
Tsp45I GTSAC 3 cut(s) 124, 214, 347
TspDTI ATGAA 4 cut(s) 64, 176, 263, 316
TspGWI ACGGA 2 cut(s) 366, 395
TspRI CASTG 2 cut(s) 151, 240
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 1 cut(s) 587
XceI RCATGY 1 cut(s) 37
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 646
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.