Rh4BG316800

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000852
Physical Location & Seq
Reverse (-)
48424393 .. 48425792
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG316800.1

Sequence Viewer

Length: 564 bp
ATGTTTCTCAACTCTTGGGCTTCCATTTCCCGAGGGGATAATTGTGATTACACAGCTCCCACTTTTGACTTGGCTACGTTCTTCCCGCCGAGATCCATATCCGGGTTCAAACCAGGGACTGGAATCGCAAGAGACAAAATCGTTACTAAACGATTCGTCTTCAGCGCCTCCACCGTCGCTTCTCTCCGAACAAAATACACGACATCTGCAACTGACAGACGCCCGACTCGTATCGAGGCCCTGTCAGCTTTTGTATGGAACCGTTTCTTGGCTTCCACGCAAGGAAAGCCCAACTCGGACACAGTTTACAAAGTTCTCCACGCAGTCAACCTACGAACCAGAATGGACCCGGCGCTTCCGGAGAGTCATTTCGGAAACGTGAGCCGGTTCGCGACTGCGACGACGTCGTTTGAGGAGGGGGAGGAGGAGTGTTATGGGTTTGTGGGACAAATGAGAGATGCCATAAAGACCGTCGACGGGGAGTACATGAGGAAGATTCTAGAAGGCACCGCGCATGTGAATTATTTGAAGCAGCGAGCGGAGGAGTTTCTGAAAGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

187

Amino Acids

21.05

Weight (kDa)

9.12

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 172 4.3e-20 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
AccB1I GGYRCC 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 539
AccI GTMKAC 1 cut(s) 474
AccII CGCG 2 cut(s) 392, 512
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 3 cut(s) 86, 510, 539
AclWI GGATC 1 cut(s) 87
AcuI CTGAAG 1 cut(s) 145
AcyI GRCGYC 2 cut(s) 220, 404
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 4 cut(s) 102, 119, 268, 477
AgsI TTSAA 2 cut(s) 109, 529
AjnI CCWGG 1 cut(s) 112
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 2 cut(s) 56, 248
AluI AGCT 2 cut(s) 56, 248
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 1 cut(s) 87
AlwNI CAGNNNCTG 1 cut(s) 119
Ama87I CYCGRG 1 cut(s) 30
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 1 cut(s) 532
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 3 cut(s) 167, 355, 514
AspS9I GGNCC 2 cut(s) 238, 346
AsuC2I CCSGG 2 cut(s) 103, 350
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 151
BbvI GCAGC 1 cut(s) 544
BciT130I CCWGG 1 cut(s) 114
BcnI CCSGG 2 cut(s) 103, 350
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 500
BfoI RGCGCY 2 cut(s) 168, 356
BisI GCNGC 1 cut(s) 533
BlsI GCNGC 1 cut(s) 534
Bme1390I CCNGG 3 cut(s) 103, 114, 350
Bme18I GGWCC 1 cut(s) 346
BmeT110I CYCGRG 1 cut(s) 30
BmgT120I GGNCC 2 cut(s) 238, 346
BmiI GGNNCC 3 cut(s) 260, 348, 508
BmrFI CCNGG 3 cut(s) 103, 114, 350
BmsI GCATC 1 cut(s) 448
BpiI GAAGAC 1 cut(s) 151
BpuMI CCSGG 2 cut(s) 103, 350
BsaBI GATNNNNATC 1 cut(s) 97
BsaHI GRCGYC 2 cut(s) 220, 404
BsaJI CCNNGG 2 cut(s) 31, 113
BsaWI WCCGGW 1 cut(s) 358
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 4 cut(s) 102, 119, 268, 477
Bse118I RCCGGY 1 cut(s) 384
Bse1I ACTGG 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 97
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 31, 113
BseJI GATNNNNATC 1 cut(s) 97
BseLI CCNNNNNNNGG 4 cut(s) 102, 119, 268, 477
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 4 cut(s) 428, 437, 440, 557
BseXI GCAGC 1 cut(s) 544
Bsh1236I CGCG 2 cut(s) 392, 512
BshFI GGCC 1 cut(s) 239
BshNI GGYRCC 1 cut(s) 506
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 4 cut(s) 102, 350, 359, 385
BslFI GGGAC 2 cut(s) 130, 459
BslI CCNNNNNNNGG 4 cut(s) 102, 119, 268, 477
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 2 cut(s) 130, 459
BsnI GGCC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 30
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 1 cut(s) 92
Bsp68I TCGCGA 1 cut(s) 392
BspACI CCGC 3 cut(s) 86, 510, 539
BspANI GGCC 1 cut(s) 239
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 2 cut(s) 392, 512
BspLI GGNNCC 3 cut(s) 260, 348, 508
BspPI GGATC 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 506
BsrBI CCGCTC 1 cut(s) 539
BsrFI RCCGGY 1 cut(s) 384
BsrI ACTGG 1 cut(s) 124
BssAI RCCGGY 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 31, 113
BssMI GATC 1 cut(s) 92
BssNI GRCGYC 2 cut(s) 220, 404
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 4 cut(s) 175, 263, 304, 472
BstACI GRCGYC 2 cut(s) 220, 404
BstC8I GCNNGC 1 cut(s) 537
BstFNI CGCG 2 cut(s) 392, 512
BstH2I RGCGCY 2 cut(s) 168, 356
BstHHI GCGC 3 cut(s) 167, 355, 514
BstKTI GATC 1 cut(s) 95
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 1 cut(s) 92
BstMWI GCNNNNNNNGC 2 cut(s) 245, 286
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 1 cut(s) 518
BstSCI CCNGG 3 cut(s) 101, 112, 348
BstUI CGCG 2 cut(s) 392, 512
BstV1I GCAGC 1 cut(s) 544
BstV2I GAAGAC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 1 cut(s) 239
BtuMI TCGCGA 1 cut(s) 392
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 119
CfoI GCGC 3 cut(s) 167, 355, 514
Cfr10I RCCGGY 1 cut(s) 384
Cfr13I GGNCC 2 cut(s) 238, 346
CseI GACGC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 484
CviAII CATG 2 cut(s) 487, 515
CviJI RGCY 8 cut(s) 20, 56, 74, 239, 248, 272, 289, 384
CviKI_1 RGCY 8 cut(s) 20, 56, 74, 239, 248, 272, 289, 384
CviQI GTAC 1 cut(s) 484
DpnI GATC 1 cut(s) 94
DpnII GATC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 30
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 2 cut(s) 490, 518
FaiI YATR 6 cut(s) 98, 256, 435, 464, 488, 516
FaqI GGGAC 2 cut(s) 130, 459
FatI CATG 2 cut(s) 486, 514
FauI CCCGC 1 cut(s) 93
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 1 cut(s) 533
Fsp4HI GCNGC 1 cut(s) 533
FspBI CTAG 1 cut(s) 500
GlaI GCGC 3 cut(s) 166, 354, 513
GluI GCNGC 1 cut(s) 533
HaeII RGCGCY 2 cut(s) 168, 356
HaeIII GGCC 1 cut(s) 239
HapII CCGG 4 cut(s) 102, 350, 359, 385
HgaI GACGC 1 cut(s) 228
HhaI GCGC 3 cut(s) 167, 355, 514
Hin1I GRCGYC 2 cut(s) 220, 404
Hin1II CATG 2 cut(s) 490, 518
Hin6I GCGC 3 cut(s) 165, 353, 512
HinP1I GCGC 3 cut(s) 165, 353, 512
HincII GTYRAC 2 cut(s) 328, 475
HindII GTYRAC 2 cut(s) 328, 475
HinfI GANTC 5 cut(s) 123, 153, 226, 364, 496
HpaII CCGG 4 cut(s) 102, 350, 359, 385
Hpy166II GTNNAC 3 cut(s) 307, 328, 475
Hpy188I TCNGA 4 cut(s) 188, 298, 374, 552
Hpy188III TCNNGA 4 cut(s) 30, 359, 391, 500
Hpy8I GTNNAC 3 cut(s) 307, 328, 475
Hpy99I CGWCG 6 cut(s) 179, 403, 406, 409, 476, 479
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 4 cut(s) 175, 263, 304, 472
HpyCH4IV ACGT 3 cut(s) 77, 378, 404
HpyCH4V TGCA 1 cut(s) 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 286
HpySE526I ACGT 3 cut(s) 77, 378, 404
Hsp92I GRCGYC 2 cut(s) 220, 404
Hsp92II CATG 2 cut(s) 490, 518
HspAI GCGC 3 cut(s) 165, 353, 512
Kpn2I TCCGGA 1 cut(s) 358
Kzo9I GATC 1 cut(s) 92
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 9 cut(s) 99, 105, 115, 126, 254, 352, 363, 372, 398
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 1 cut(s) 448
MaeI CTAG 1 cut(s) 500
MaeII ACGT 3 cut(s) 77, 378, 404
MaeIII GTNAC 1 cut(s) 142
MalI GATC 1 cut(s) 94
MbiI CCGCTC 1 cut(s) 539
MboI GATC 1 cut(s) 92
MboII GAAGA 3 cut(s) 73, 151, 505
MflI RGATCY 1 cut(s) 92
MluCI AATT 2 cut(s) 40, 520
MlyI GAGTC 2 cut(s) 220, 373
MnlI CCTC 9 cut(s) 26, 178, 229, 406, 409, 415, 418, 483, 535
MroI TCCGGA 1 cut(s) 358
MroXI GAANNNNTTC 1 cut(s) 263
MspI CCGG 4 cut(s) 102, 350, 359, 385
MspR9I CCNGG 3 cut(s) 103, 114, 350
MvaI CCWGG 1 cut(s) 114
MvnI CGCG 2 cut(s) 392, 512
MwoI GCNNNNNNNGC 2 cut(s) 245, 286
NciI CCSGG 2 cut(s) 103, 350
NdeII GATC 1 cut(s) 92
NlaIII CATG 2 cut(s) 490, 518
NlaIV GGNNCC 3 cut(s) 260, 348, 508
NmeAIII GCCGAG 1 cut(s) 114
NruI TCGCGA 1 cut(s) 392
NspI RCATGY 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 123, 153, 496
PflFI GACNNNGTC 1 cut(s) 403
PflMI CCANNNNNTGG 1 cut(s) 119
PkrI GCNGC 1 cut(s) 534
PleI GAGTC 2 cut(s) 220, 372
PpsI GAGTC 2 cut(s) 220, 372
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 3 cut(s) 260, 348, 508
PspPI GGNCC 2 cut(s) 238, 346
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 92
PsyI GACNNNGTC 1 cut(s) 403
RruI TCGCGA 1 cut(s) 392
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
SalI GTCGAC 1 cut(s) 473
SatI GCNGC 1 cut(s) 533
Sau3AI GATC 1 cut(s) 92
Sau96I GGNCC 2 cut(s) 238, 346
SchI GAGTC 2 cut(s) 220, 373
ScrFI CCNGG 3 cut(s) 103, 114, 350
SetI ASST 6 cut(s) 58, 80, 250, 333, 381, 407
SfaNI GCATC 1 cut(s) 448
SgrDI CGTCGACG 1 cut(s) 473
SinI GGWCC 1 cut(s) 346
Sse9I AATT 2 cut(s) 40, 520
SsiI CCGC 3 cut(s) 86, 510, 539
SspMI CTAG 1 cut(s) 500
StyD4I CCNGG 3 cut(s) 101, 112, 348
TaaI ACNGT 4 cut(s) 175, 263, 304, 472
TaiI ACGT 3 cut(s) 80, 381, 407
TaqI TCGA 2 cut(s) 234, 474
TasI AATT 2 cut(s) 40, 520
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 3 cut(s) 123, 153, 496
TseI GCWGC 1 cut(s) 532
Tth111I GACNNNGTC 1 cut(s) 403
Van91I CCANNNNNTGG 1 cut(s) 119
VpaK11BI GGWCC 1 cut(s) 346
XbaI TCTAGA 1 cut(s) 499
XceI RCATGY 1 cut(s) 518
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 474
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 1 cut(s) 500
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.