Rh4CG332200

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000852
Physical Location & Seq
Reverse (-)
59336056 .. 59337131
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG332200.1

Sequence Viewer

Length: 411 bp
ATGGTTCAGGCCATCCGAGCCAAATGCATCGACATGTCCAGACGCCCCACTCGTGTTGAAGCCTTATCAGCCTTCATATGGAGCCGCTACATAGCATCATGCACCGGCATTCAACGAGATGGCGTCACCACCAACGCGACGTACACTGTTTCTCATGCTGTGAACCTCCGCACGAGAATGGAGCCACCACTTCCGGAGTACACATTCGGAAACGTGACCCGAACCGCCATTGCGGTGCCGCCCGTACCCGTTGACCCGAAAGACGGGACGCTTCATGGGGTGGTTAATCACGTCAGGGAGGCCATTAAGCAAGTAGACAAAGAGTACATAAAGAAACTGCAAGAGGGTGACGAACACTTGAGCTTCTTGAGGAGCCGTTCGGAGGAGGTGAAACGCGGAGAGCATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

136

Amino Acids

15.36

Weight (kDa)

9.26

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 14 - 118 5.7e-11 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 315
AccII CGCG 2 cut(s) 137, 396
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 6 cut(s) 85, 169, 225, 233, 239, 396
AcyI GRCGYC 2 cut(s) 43, 123
AfaI GTAC 4 cut(s) 143, 200, 246, 326
AfiI CCNNNNNNNGG 3 cut(s) 78, 263, 382
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 2 cut(s) 59, 113
AjiI CACGTC 1 cut(s) 292
AluBI AGCT 1 cut(s) 363
AluI AGCT 1 cut(s) 363
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 2 cut(s) 9, 300
AsuHPI GGTGA 3 cut(s) 118, 359, 400
BanI GGYRCC 1 cut(s) 235
BauI CACGAG 2 cut(s) 51, 172
BccI CCATC 2 cut(s) 20, 113
BceAI ACGGC 1 cut(s) 360
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BisI GCNGC 2 cut(s) 85, 239
BlsI GCNGC 2 cut(s) 86, 240
BmgBI CACGTC 1 cut(s) 292
BmiI GGNNCC 4 cut(s) 83, 183, 237, 374
BmsI GCATC 2 cut(s) 36, 104
BpuEI CTTGAG 2 cut(s) 379, 388
BsaHI GRCGYC 2 cut(s) 43, 123
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 263, 382
Bse118I RCCGGY 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 228
BseAI TCCGGA 1 cut(s) 193
BseGI GGATG 1 cut(s) 12
BseLI CCNNNNNNNGG 3 cut(s) 78, 263, 382
BseMI GCAATG 1 cut(s) 228
BseRI GAGGAG 2 cut(s) 385, 398
Bsh1236I CGCG 2 cut(s) 137, 396
BshFI GGCC 2 cut(s) 11, 302
BshNI GGYRCC 1 cut(s) 235
BsiSI CCGG 2 cut(s) 105, 194
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 3 cut(s) 78, 263, 382
BsmFI GGGAC 1 cut(s) 280
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 2 cut(s) 11, 302
Bsp13I TCCGGA 1 cut(s) 193
BspACI CCGC 6 cut(s) 85, 169, 225, 233, 239, 396
BspANI GGCC 2 cut(s) 11, 302
BspEI TCCGGA 1 cut(s) 193
BspFNI CGCG 2 cut(s) 137, 396
BspLI GGNNCC 4 cut(s) 83, 183, 237, 374
BspT107I GGYRCC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 104
BssNI GRCGYC 2 cut(s) 43, 123
BssSI CACGAG 2 cut(s) 51, 172
Bst2BI CACGAG 2 cut(s) 51, 172
Bst4CI ACNGT 1 cut(s) 148
BstACI GRCGYC 2 cut(s) 43, 123
BstF5I GGATG 1 cut(s) 12
BstFNI CGCG 2 cut(s) 137, 396
BstMWI GCNNNNNNNGC 2 cut(s) 17, 68
BstNSI RCATGY 1 cut(s) 37
BstUI CGCG 2 cut(s) 137, 396
BsuRI GGCC 2 cut(s) 11, 302
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 104
CseI GACGC 3 cut(s) 51, 112, 277
Csp6I GTAC 4 cut(s) 142, 199, 245, 325
CspCI CAANNNNNGTGG 2 cut(s) 37, 72
CviAII CATG 4 cut(s) 34, 99, 155, 275
CviJI RGCY 9 cut(s) 11, 20, 62, 71, 84, 184, 302, 363, 375
CviKI_1 RGCY 9 cut(s) 11, 20, 62, 71, 84, 184, 302, 363, 375
CviQI GTAC 4 cut(s) 142, 199, 245, 325
EcoT22I ATGCAT 1 cut(s) 29
FaeI CATG 4 cut(s) 37, 102, 158, 278
FaiI YATR 9 cut(s) 35, 77, 79, 92, 100, 156, 276, 329, 405
FaqI GGGAC 1 cut(s) 280
FatI CATG 4 cut(s) 33, 98, 154, 274
FauNDI CATATG 1 cut(s) 77
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 2 cut(s) 85, 239
Fsp4HI GCNGC 2 cut(s) 85, 239
GluI GCNGC 2 cut(s) 85, 239
HaeIII GGCC 2 cut(s) 11, 302
HapII CCGG 2 cut(s) 105, 194
HgaI GACGC 3 cut(s) 51, 112, 277
Hin1I GRCGYC 2 cut(s) 43, 123
Hin1II CATG 4 cut(s) 37, 102, 158, 278
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HpaII CCGG 2 cut(s) 105, 194
HphI GGTGA 3 cut(s) 118, 359, 400
Hpy166II GTNNAC 5 cut(s) 144, 163, 201, 253, 316
Hpy188I TCNGA 4 cut(s) 17, 209, 382, 410
Hpy188III TCNNGA 3 cut(s) 39, 194, 367
Hpy8I GTNNAC 5 cut(s) 144, 163, 201, 253, 316
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 3 cut(s) 140, 213, 291
HpyCH4V TGCA 3 cut(s) 27, 102, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 68
HpySE526I ACGT 3 cut(s) 140, 213, 291
Hsp92I GRCGYC 2 cut(s) 43, 123
Hsp92II CATG 4 cut(s) 37, 102, 158, 278
Kpn2I TCCGGA 1 cut(s) 193
LmnI GCTCC 3 cut(s) 81, 181, 372
LpnPI CCDG 4 cut(s) 52, 118, 207, 280
LweI GCATC 2 cut(s) 36, 104
MaeII ACGT 3 cut(s) 140, 213, 291
MaeIII GTNAC 3 cut(s) 124, 214, 347
MnlI CCTC 6 cut(s) 176, 292, 337, 363, 376, 379
Mph1103I ATGCAT 1 cut(s) 29
MroI TCCGGA 1 cut(s) 193
MseI TTAA 2 cut(s) 285, 306
MslI CAYNNNNRTG 3 cut(s) 32, 176, 233
MspI CCGG 2 cut(s) 105, 194
Mva1269I GAATGC 1 cut(s) 108
MvnI CGCG 2 cut(s) 137, 396
MwoI GCNNNNNNNGC 2 cut(s) 17, 68
NdeI CATATG 1 cut(s) 77
NlaIII CATG 4 cut(s) 37, 102, 158, 278
NlaIV GGNNCC 4 cut(s) 83, 183, 237, 374
NmuCI GTSAC 3 cut(s) 124, 214, 347
NsiI ATGCAT 1 cut(s) 29
NspI RCATGY 1 cut(s) 37
PciI ACATGT 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 49
PctI GAATGC 1 cut(s) 108
PkrI GCNGC 2 cut(s) 86, 240
PscI ACATGT 1 cut(s) 33
PspN4I GGNNCC 4 cut(s) 83, 183, 237, 374
RsaI GTAC 4 cut(s) 143, 200, 246, 326
RsaNI GTAC 4 cut(s) 142, 199, 245, 325
RseI CAYNNNNRTG 3 cut(s) 32, 176, 233
SaqAI TTAA 2 cut(s) 285, 306
SatI GCNGC 2 cut(s) 85, 239
SetI ASST 6 cut(s) 143, 168, 216, 294, 365, 390
SfaNI GCATC 2 cut(s) 36, 104
SmiMI CAYNNNNRTG 3 cut(s) 32, 176, 233
SmlI CTYRAG 2 cut(s) 358, 367
SmoI CTYRAG 2 cut(s) 358, 367
SsiI CCGC 6 cut(s) 85, 169, 225, 233, 239, 396
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 3 cut(s) 143, 216, 294
TaqI TCGA 1 cut(s) 30
TatI WGTACW 2 cut(s) 198, 324
TauI GCSGC 2 cut(s) 87, 241
Tru1I TTAA 2 cut(s) 285, 306
Tru9I TTAA 2 cut(s) 285, 306
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 3 cut(s) 124, 214, 347
Tsp45I GTSAC 3 cut(s) 124, 214, 347
TspDTI ATGAA 2 cut(s) 64, 263
TspRI CASTG 1 cut(s) 151
XceI RCATGY 1 cut(s) 37
XmiI GTMKAC 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.